##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937329_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415747 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28951501754673 33.0 31.0 34.0 31.0 34.0 2 32.47526019430086 34.0 31.0 34.0 31.0 34.0 3 32.502909221233104 34.0 31.0 34.0 31.0 34.0 4 36.13554397265645 37.0 35.0 37.0 35.0 37.0 5 36.070324019175125 37.0 35.0 37.0 35.0 37.0 6 36.18945175792008 37.0 36.0 37.0 35.0 37.0 7 36.153275910589855 37.0 36.0 37.0 35.0 37.0 8 36.148292110345956 37.0 36.0 37.0 35.0 37.0 9 37.849761994674644 39.0 38.0 39.0 35.0 39.0 10-11 37.7834764893072 39.0 37.0 39.0 35.0 39.0 12-13 37.70608928025939 39.0 37.0 39.0 35.0 39.0 14-15 38.78774591277869 40.0 38.0 41.0 35.0 41.0 16-17 38.795261300743 40.0 38.0 41.0 35.5 41.0 18-19 38.938819762980856 40.0 38.0 41.0 35.5 41.0 20-21 38.90662109407885 40.0 38.0 41.0 35.0 41.0 22-23 38.780129501836456 40.0 38.0 41.0 35.0 41.0 24-25 38.644739468955876 40.0 38.0 41.0 35.0 41.0 26-27 38.526162545971474 40.0 38.0 41.0 34.5 41.0 28-29 38.37938818560326 40.0 38.0 41.0 34.0 41.0 30-31 38.25032171007848 40.0 37.0 41.0 34.0 41.0 32-33 37.988995711334056 40.0 36.5 41.0 34.0 41.0 34-35 37.80347543097124 40.0 36.0 41.0 33.5 41.0 36-37 37.57990316225974 40.0 35.0 41.0 33.0 41.0 38-39 37.39597038583562 40.0 35.0 41.0 33.0 41.0 40-41 37.14761381320851 40.0 35.0 41.0 33.0 41.0 42-43 36.816658929589394 39.5 35.0 41.0 32.5 41.0 44-45 36.308426759543664 39.0 35.0 41.0 31.0 41.0 46-47 35.61813194081978 39.0 35.0 41.0 28.5 41.0 48-49 35.052477829064316 39.0 35.0 41.0 24.5 41.0 50-51 34.72917663867689 39.0 35.0 41.0 23.0 41.0 52-53 34.461193947280435 39.0 35.0 40.0 21.5 41.0 54-55 34.23283030304488 38.0 35.0 40.0 20.0 41.0 56-57 33.985598212374356 38.0 34.0 40.0 18.5 41.0 58-59 33.74933793869829 38.0 34.0 40.0 15.5 41.0 60-61 33.424612805384044 37.5 34.0 40.0 10.0 41.0 62-63 33.032582315687186 37.0 33.0 40.0 7.0 41.0 64-65 32.65995184571386 36.5 33.0 39.5 2.0 41.0 66-67 32.22651275896158 36.0 33.0 39.0 2.0 40.5 68-69 31.830493064291502 35.5 32.0 39.0 2.0 40.0 70-71 31.397572321628296 35.0 32.0 38.5 2.0 40.0 72-73 30.936692267172102 35.0 31.0 37.5 2.0 40.0 74-75 30.477859130673224 35.0 31.0 37.0 2.0 39.0 76-77 29.152539886036458 33.5 29.5 35.5 2.0 38.5 78-79 29.69782584119669 34.0 30.0 36.0 2.0 38.5 80-81 29.52744818363091 34.5 30.5 36.0 2.0 37.5 82-83 29.184232237394376 34.0 30.0 35.0 2.0 37.0 84-85 28.79797088132927 34.0 29.5 35.0 2.0 36.5 86-87 28.39128845187097 34.0 29.0 35.0 2.0 36.0 88-89 27.964951039935343 34.0 29.0 35.0 2.0 36.0 90-91 27.51368500554424 34.0 27.5 35.0 2.0 35.0 92-93 27.153032974381055 34.0 27.0 35.0 2.0 35.0 94-95 26.75271018191352 33.5 25.5 35.0 2.0 35.0 96-97 26.264242435904528 33.0 25.0 35.0 2.0 35.0 98-99 25.826863453013495 33.0 23.5 35.0 2.0 35.0 100-101 24.123470524140885 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18672666027602958 1101 2 0.1616682925300097 1101 3 0.10807564227339128 1101 4 0.15736839075413656 1101 5 0.03725518920041537 1101 6 0.0628969280155971 1101 7 0.0750925487563876 1101 8 -0.017541249846715345 1101 9 0.1380805436266641 1101 10-11 0.02343322067534359 1101 12-13 -0.05580398160340394 1101 14-15 -0.023264621596673862 1101 16-17 0.024565725553529205 1101 18-19 0.017775020484641857 1101 20-21 0.14494255103848275 1101 22-23 0.15462339397298308 1101 24-25 0.08165686786373527 1101 26-27 0.09934800920120068 1101 28-29 0.14996517732554082 1101 30-31 0.09391713500061627 1101 32-33 0.02801285739454329 1101 34-35 -0.04610611310108226 1101 36-37 -0.06435778532712533 1101 38-39 0.016228828259002626 1101 40-41 -0.08467537673599423 1101 42-43 -0.05129050825502901 1101 44-45 0.04181714946194859 1101 46-47 0.13417507646256865 1101 48-49 0.16512414195572234 1101 50-51 0.16255222239686162 1101 52-53 0.10554927140204029 1101 54-55 0.40016007014772725 1101 56-57 0.5780581427175591 1101 58-59 0.38143791233920865 1101 60-61 0.3491837250254122 1101 62-63 0.32596752180197797 1101 64-65 0.38891538629522415 1101 66-67 0.3356141517205451 1101 68-69 0.20581321698187338 1101 70-71 -0.13940557386088415 1101 72-73 -0.11035436292407752 1101 74-75 0.07857039114662001 1101 76-77 0.2577595184576644 1101 78-79 0.4856037938188713 1101 80-81 0.5673415415657388 1101 82-83 0.26436782349752974 1101 84-85 0.42372242143697747 1101 86-87 0.4373572282296756 1101 88-89 0.582226762284737 1101 90-91 0.3526478907230839 1101 92-93 0.33499559133215584 1101 94-95 0.3676158999354584 1101 96-97 0.1874249439580673 1101 98-99 0.2891056869145565 1101 100-101 0.7939100555572978 1102 1 -0.05682346497464863 1102 2 0.025890846216519492 1102 3 -0.17380261834794197 1102 4 0.05409545846087127 1102 5 -0.02892070414738157 1102 6 -0.02760114861926155 1102 7 -0.0861346938017249 1102 8 0.08320882687378628 1102 9 0.027713641208009676 1102 10-11 -0.013340878483226959 1102 12-13 -0.062177135850866705 1102 14-15 -0.15212651755654605 1102 16-17 -0.20456094534763736 1102 18-19 -0.018328220332087142 1102 20-21 0.09934737836383079 1102 22-23 0.002834557341266475 1102 24-25 -0.17921781722537133 1102 26-27 -0.04565017091713486 1102 28-29 0.020229024484024194 1102 30-31 -0.04527704994217885 1102 32-33 -0.2994926914603582 1102 34-35 -0.07815208830786702 1102 36-37 -0.1791798294467597 1102 38-39 -0.3318913410256812 1102 40-41 -0.38726995705700773 1102 42-43 -0.3409746338910935 1102 44-45 -0.4748668833958334 1102 46-47 -0.4854767908787636 1102 48-49 -0.655112585397454 1102 50-51 -0.861564778152669 1102 52-53 -0.7409062414120697 1102 54-55 -0.9455799793816055 1102 56-57 -0.9130619052416833 1102 58-59 -0.9231442642392693 1102 60-61 -0.9139800857354459 1102 62-63 -0.9940872173839956 1102 64-65 -0.9987961303442034 1102 66-67 -0.6400993489467446 1102 68-69 -0.7996999539656784 1102 70-71 -0.3906348860883462 1102 72-73 -0.5680474666430584 1102 74-75 -0.6772086252608638 1102 76-77 -0.7334122712353839 1102 78-79 -0.47163077108667295 1102 80-81 -0.3944284539380831 1102 82-83 -0.3540333212437261 1102 84-85 -0.3176260038301173 1102 86-87 -0.30557734880841636 1102 88-89 -0.32503599294474483 1102 90-91 -0.3077693260712131 1102 92-93 -0.29655152219277525 1102 94-95 -0.14973007027557728 1102 96-97 -0.5905940969999754 1102 98-99 -0.3872194367303443 1102 100-101 0.07070692107874521 1103 1 0.09454397389279023 1103 2 -0.013539655713984189 1103 3 0.05410922206390012 1103 4 0.09330877892418954 1103 5 -0.04771092293760404 1103 6 0.030056509038395518 1103 7 0.017895228978197508 1103 8 0.10215992082488157 1103 9 0.0725575923019619 1103 10-11 0.012881772739426367 1103 12-13 0.0417160430423138 1103 14-15 0.030016339586317997 1103 16-17 -0.06211403415072425 1103 18-19 0.1888990962702266 1103 20-21 0.03799361388506384 1103 22-23 -0.06201824188653404 1103 24-25 0.12240702189946973 1103 26-27 0.022613181721219178 1103 28-29 -0.016828568823569867 1103 30-31 0.026457184292048908 1103 32-33 -0.03457956404722751 1103 34-35 -0.21658516424094643 1103 36-37 -0.09371892211085253 1103 38-39 -0.13095424321357996 1103 40-41 -0.10649453878158965 1103 42-43 -0.2062215772630296 1103 44-45 -0.5768134728424243 1103 46-47 -0.572687215589184 1103 48-49 -0.5005316651915379 1103 50-51 -0.704526489864385 1103 52-53 -0.7430740342048665 1103 54-55 -0.8141691042207313 1103 56-57 -1.024379472809251 1103 58-59 -1.0470664006614072 1103 60-61 -0.8379947253750828 1103 62-63 -0.7215043666761431 1103 64-65 -0.5585853838834609 1103 66-67 -0.7425647607871575 1103 68-69 -0.5647216721123982 1103 70-71 -0.6984493970278614 1103 72-73 -0.4712408385864322 1103 74-75 -0.5694463987486387 1103 76-77 -0.44813234845545935 1103 78-79 -0.4179467299026314 1103 80-81 -0.6245523278119549 1103 82-83 -0.73406871377912 1103 84-85 -0.32108079790991084 1103 86-87 -0.11764700775307446 1103 88-89 0.00902127861252211 1103 90-91 0.001754483452593547 1103 92-93 -0.10165528667154078 1103 94-95 -0.20508319125369567 1103 96-97 -0.41727122055209875 1103 98-99 -0.022354571865481176 1103 100-101 -0.011685292563470284 1104 1 0.08428681998714183 1104 2 -0.03984476012233529 1104 3 0.09391007279862862 1104 4 -1.5119787395434514E-4 1104 5 0.06048706623486311 1104 6 -0.0640656874039891 1104 7 -0.16103458540695215 1104 8 -0.07960728721842969 1104 9 -0.09350485936084141 1104 10-11 0.02283923600315063 1104 12-13 -0.04608364759960892 1104 14-15 -0.08616695507726035 1104 16-17 0.05466380807665416 1104 18-19 0.04943418907765107 1104 20-21 -0.18195273182259086 1104 22-23 0.02495246577007748 1104 24-25 0.06787299251045198 1104 26-27 0.04790078806306752 1104 28-29 -0.03402211097360919 1104 30-31 0.12495873108246514 1104 32-33 0.04775802295973364 1104 34-35 0.07812674604521419 1104 36-37 0.1881328760330021 1104 38-39 -0.19444273895990705 1104 40-41 0.02191806756025727 1104 42-43 0.08900995792645006 1104 44-45 0.2050214072813219 1104 46-47 0.21742299322751535 1104 48-49 0.407978098922932 1104 50-51 0.224674012842506 1104 52-53 0.2671517005205857 1104 54-55 0.34238058642119285 1104 56-57 0.3494169309030468 1104 58-59 0.4123980231591631 1104 60-61 0.5904251315468088 1104 62-63 0.4066979728443485 1104 64-65 0.46943569113587813 1104 66-67 0.6008057921904175 1104 68-69 0.4021069241753068 1104 70-71 0.23454315904329448 1104 72-73 -0.13565982543871158 1104 74-75 -0.1753677057865346 1104 76-77 0.05827716430556151 1104 78-79 0.10764298394114036 1104 80-81 0.09809365053297725 1104 82-83 0.10165247724949822 1104 84-85 0.031759012608265635 1104 86-87 0.035671341048161054 1104 88-89 0.11376799168290219 1104 90-91 9.8108824764509E-4 1104 92-93 0.08572145346767002 1104 94-95 0.07654625576872931 1104 96-97 0.5149721089142574 1104 98-99 0.10873591537349725 1104 100-101 0.9966625419299575 1105 1 0.1283566582585678 1105 2 0.18767715844531097 1105 3 0.09286290937953368 1105 4 0.06924904441091684 1105 5 0.14904423930428834 1105 6 0.1625048866195158 1105 7 0.1168812171787863 1105 8 0.04146670548564657 1105 9 0.10122647135800378 1105 10-11 0.16811776094001374 1105 12-13 0.03412017283582003 1105 14-15 0.18879215079575573 1105 16-17 0.35855336997906306 1105 18-19 0.3196605269486881 1105 20-21 0.4329512097552737 1105 22-23 0.4676185575234939 1105 24-25 0.2380781133448977 1105 26-27 0.22586723776841922 1105 28-29 0.3575998972531238 1105 30-31 0.3047388952065049 1105 32-33 0.36700598167548293 1105 34-35 0.36730126643752214 1105 36-37 0.6536723020779363 1105 38-39 0.5675431756654774 1105 40-41 0.5140438093010076 1105 42-43 0.24146463217649483 1105 44-45 0.296305996184131 1105 46-47 0.3567744363282159 1105 48-49 0.4796951047199727 1105 50-51 0.6206763124954975 1105 52-53 0.8040590778895336 1105 54-55 0.44064284268192466 1105 56-57 0.44488675137954203 1105 58-59 0.3246518884241354 1105 60-61 0.5297136079582572 1105 62-63 0.4281545566276961 1105 64-65 0.527163141190286 1105 66-67 0.5380307406877769 1105 68-69 0.5514748618109024 1105 70-71 0.6574226531196352 1105 72-73 0.33729529710678463 1105 74-75 0.356088262018325 1105 76-77 0.6259458226359875 1105 78-79 0.617488373342205 1105 80-81 0.6011666987367157 1105 82-83 0.6597734691117374 1105 84-85 0.6832794528275521 1105 86-87 0.40937179755666264 1105 88-89 0.5460788007364172 1105 90-91 0.7318429790278174 1105 92-93 0.41315850388892983 1105 94-95 0.41513251671090146 1105 96-97 0.2821664638136845 1105 98-99 0.6546365785769943 1105 100-101 0.9204865363450878 1106 1 0.029030443757484647 1106 2 -0.1586964822822452 1106 3 0.0054776107477891856 1106 4 -0.10330099967359985 1106 5 -0.13270477287610305 1106 6 -0.33980818960858983 1106 7 -0.015753295007037593 1106 8 0.024638395609628105 1106 9 0.11595396376567635 1106 10-11 -0.11826828876119322 1106 12-13 -0.10960622018031785 1106 14-15 -0.09756668624394393 1106 16-17 -0.18266292713965981 1106 18-19 -0.11777756424637431 1106 20-21 -0.11254836028467707 1106 22-23 0.03755509759686504 1106 24-25 0.0018504413336657421 1106 26-27 -0.3307731958925544 1106 28-29 -0.17947463278421338 1106 30-31 -0.11147332001299048 1106 32-33 -0.24495779282951702 1106 34-35 -0.6101122245115462 1106 36-37 -0.6749804534761665 1106 38-39 -0.7897372997941403 1106 40-41 -1.0246525953621486 1106 42-43 -1.3172494062913245 1106 44-45 -1.3284586142938366 1106 46-47 -0.9138772524895558 1106 48-49 -1.0230370464953467 1106 50-51 -1.6873601307007888 1106 52-53 -1.6118048152626727 1106 54-55 -1.7619935199648822 1106 56-57 -1.6642088086026021 1106 58-59 -1.795421259209995 1106 60-61 -1.5557722469002968 1106 62-63 -1.213797570858187 1106 64-65 -1.6142665031946493 1106 66-67 -1.8114723561131107 1106 68-69 -1.580577299418671 1106 70-71 -1.479680178085669 1106 72-73 -1.058687025548302 1106 74-75 -1.3332302240868898 1106 76-77 -1.5726249683816604 1106 78-79 -1.3120580460157925 1106 80-81 -1.3215003597922745 1106 82-83 -1.4867286153781336 1106 84-85 -1.6295409762616977 1106 86-87 -1.6308811713447113 1106 88-89 -1.566701353613798 1106 90-91 -1.7257977920701606 1106 92-93 -2.043502611394789 1106 94-95 -2.3764892490642744 1106 96-97 -2.037946958559477 1106 98-99 -1.634900697326735 1106 100-101 -1.3003768669792137 1107 1 -0.008637029691442422 1107 2 0.2300535342501746 1107 3 0.1630249926732219 1107 4 0.15771290078799183 1107 5 0.06949337813766476 1107 6 0.2411676201495041 1107 7 0.10904666266995378 1107 8 0.19866529716896508 1107 9 0.09529504749909279 1107 10-11 0.09755919209425912 1107 12-13 0.23678772763013 1107 14-15 0.2749088127045951 1107 16-17 0.3105786181790222 1107 18-19 0.24003036867166117 1107 20-21 0.4486678970391864 1107 22-23 0.15880389072995627 1107 24-25 0.23696795379910895 1107 26-27 0.22992724982157853 1107 28-29 0.3874949078789385 1107 30-31 -0.12914317413088838 1107 32-33 -0.11921218053468863 1107 34-35 -0.39691670545958146 1107 36-37 -0.49496220168074245 1107 38-39 -0.47511866973687233 1107 40-41 -0.5379909545521997 1107 42-43 -0.7028053586250351 1107 44-45 -0.9361772721255761 1107 46-47 -1.5929829145139252 1107 48-49 -1.7340106078438637 1107 50-51 -1.498903730007747 1107 52-53 -1.6483943747066547 1107 54-55 -1.6053653407798691 1107 56-57 -1.744966390371971 1107 58-59 -1.7020395189409783 1107 60-61 -1.463117933260385 1107 62-63 -1.4955299043105548 1107 64-65 -1.5435540218762895 1107 66-67 -1.175595674765649 1107 68-69 -1.2561979265926837 1107 70-71 -1.656054683766211 1107 72-73 -1.6908868959340992 1107 74-75 -1.7517716675658406 1107 76-77 -1.583033782147215 1107 78-79 -1.7933499557090826 1107 80-81 -1.876725266880058 1107 82-83 -1.8012282119870022 1107 84-85 -1.600953109737869 1107 86-87 -1.5478553410864073 1107 88-89 -1.4360952088426728 1107 90-91 -1.510790319414788 1107 92-93 -1.7411378135532622 1107 94-95 -1.5897046070243057 1107 96-97 -1.3422824212689441 1107 98-99 -1.1943975826181692 1107 100-101 -0.7809281241046904 1108 1 -0.19945785760904755 1108 2 -0.17658910626343527 1108 3 -0.07423326877859182 1108 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-1.6104250842418892 2316 52-53 -1.7365102791847846 2316 54-55 -1.571423120284983 2316 56-57 -1.9796254567449907 2316 58-59 -2.1067551556814905 2316 60-61 -1.922093620957412 2316 62-63 -1.875307077519519 2316 64-65 -1.7694036569758254 2316 66-67 -1.4816586362891648 2316 68-69 -1.915437033558181 2316 70-71 -1.371453915100151 2316 72-73 -1.3958141317591206 2316 74-75 -1.6033068405157067 2316 76-77 -1.2564154809855808 2316 78-79 -1.9194025531958019 2316 80-81 -1.7432019262055292 2316 82-83 -1.9202634928955824 2316 84-85 -1.541424673411921 2316 86-87 -1.6864095579538763 2316 88-89 -1.590627315764987 2316 90-91 -1.7633057575112616 2316 92-93 -1.640347555747848 2316 94-95 -1.4193025687637366 2316 96-97 -1.5043068631223804 2316 98-99 -1.7412300738735134 2316 100-101 -1.4983569793104579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 10.0 6 12.0 7 52.0 8 130.0 9 213.0 10 419.0 11 791.0 12 1292.0 13 1982.0 14 2468.0 15 3755.0 16 5722.0 17 8958.0 18 7736.0 19 3855.0 20 2757.0 21 2656.0 22 2724.0 23 2830.0 24 3164.0 25 3467.0 26 3998.0 27 4599.0 28 5487.0 29 6849.0 30 8234.0 31 10205.0 32 12856.0 33 17181.0 34 24504.0 35 38706.0 36 54824.0 37 86508.0 38 78256.0 39 8545.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01386241552591 20.309401413196248 16.50854848957431 18.168187681703536 2 15.596264074064276 23.36204470507304 40.15663372195109 20.885057498911596 3 18.41552675064402 29.676461886676268 32.085378848193734 19.822632514485974 4 11.767974272814957 19.5198041116352 38.80003944706757 29.912182168482275 5 11.417257481313255 40.31982178127308 33.46364535243186 14.799275384981799 6 26.94354980312546 39.69529545613077 17.59603797501846 15.76511676572531 7 24.876667781126503 37.73520915364392 21.120056188018193 16.268066877211382 8 26.42472465225245 33.92568076257916 19.837785961173502 19.81180862399488 9 28.275850457128975 16.94612348375334 20.750360195022456 34.02766586409523 10-11 23.701794601043424 29.104719937846816 26.288584162964497 20.90490129814527 12-13 26.91880267867452 27.336984759265675 27.62070392179502 18.123508640264788 14-15 23.817249432948405 27.178789023131856 28.897382302217455 20.106579241702285 16-17 18.845836530389874 30.927583361996597 29.55499378227624 20.671586325337284 18-19 18.585461831354163 27.478731055185 35.68732907272933 18.248478040731502 20-21 20.331475633017195 26.265853992933202 35.96466120020108 17.438009173848517 22-23 19.946385340034563 26.25699197463827 35.611415184085416 18.18520750124175 24-25 19.41601502837062 26.593096282113883 36.16586529788549 17.825023391630005 26-27 19.31640378359201 26.637882824103865 36.12500526167038 17.920708130633745 28-29 18.850469650137708 26.142614886887078 37.12701600779343 17.879899455181786 30-31 18.711740553750236 27.243131038828906 36.41613769912952 17.62899070829134 32-33 18.54962272728366 27.65828737188723 35.68865199267824 18.10343790815087 34-35 18.917153942181184 26.77277286426601 36.10176381308825 18.20830938046456 36-37 19.428642900610228 26.92250334939278 35.43934171503342 18.20951203496357 38-39 19.092861764486575 27.0782471070146 35.97488376344267 17.85400736505615 40-41 18.689250914618746 27.3368178243018 36.104770449335774 17.869160811743683 42-43 18.7536235370533 27.9383427354848 34.97828884852713 18.32974487893477 44-45 19.086126899292115 28.07657060664298 34.14853264124576 18.688769852819142 46-47 19.203746509295318 28.42113112060941 33.15886825401025 19.216254116085025 48-49 19.707297948030895 28.704356255126314 32.5409443724188 19.047401424423988 50-51 20.28938272555184 29.02895270440917 31.735887450781362 18.945777119257627 52-53 19.56321993904947 29.484518228634244 31.821997512910492 19.130264319405793 54-55 19.257505165401074 29.473093010893642 31.778100623696627 19.49130120000866 56-57 19.88781638833233 29.8215020192569 30.83750454002073 19.453177052390036 58-59 19.723774314667335 29.047593849143833 31.53311990224824 19.695511933940594 60-61 19.81890428553904 29.149578950659897 31.680565343826895 19.350951419974166 62-63 20.490226026886543 29.077900742518885 31.74598974857305 18.685883482021516 64-65 20.18607470408686 28.809107461990102 32.18098988086505 18.82382795305799 66-67 19.7789761561719 29.37796304002194 31.66228499544194 19.180775808364224 68-69 19.816619241990924 29.784339995237485 31.370881810331763 19.028158952439824 70-71 19.754802800741796 29.83725679319394 31.06925606198 19.33868434408426 72-73 19.7485964988238 29.45139580416889 30.739441870757627 20.060565826249682 74-75 19.665926633264945 29.88464138045494 30.71495404657159 19.734477939708526 76-77 19.87398586159371 28.93887388243331 31.66493083533976 19.52220942063322 78-79 19.379334066150808 28.812234363687534 31.790608230486328 20.01782333967533 80-81 19.25570118365256 30.414891749068545 30.791322607258742 19.538084460020155 82-83 19.29485707001038 30.006458277960917 30.369420715317425 20.329263936711282 84-85 19.16287426878079 29.240229564347292 30.37434094057882 21.222555226293103 86-87 19.517519068087083 29.51711016555742 30.364380260110114 20.600990506245385 88-89 19.75023271364556 28.390102634534948 30.885971516330844 20.97369313548865 90-91 19.20520083473157 28.445823635893458 31.367143570221494 20.98183195915348 92-93 19.382309963499043 28.87231880310529 30.769647077217265 20.975724156178405 94-95 18.911501466035833 28.65817432236432 30.926741503847293 21.503582707752553 96-97 19.429725289659334 28.53309825446726 30.09510591778173 21.942070538091677 98-99 19.304020493331247 28.82740622256431 30.184127289564515 21.68444599453992 100-101 19.21000656744137 29.20863534341945 30.380911599352395 21.20044648978679 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1895.0 1 1250.0 2 771.5 3 937.0 4 762.0 5 737.5 6 1270.5 7 2580.0 8 3905.5 9 4235.0 10 3388.5 11 2439.0 12 2266.5 13 2242.5 14 2203.0 15 2117.5 16 2069.5 17 2014.5 18 2008.5 19 2073.0 20 2078.0 21 2190.5 22 2382.0 23 2595.5 24 2783.0 25 2960.0 26 3326.5 27 3703.0 28 3967.5 29 4679.0 30 5685.0 31 6419.0 32 7639.0 33 9251.0 34 10451.5 35 11382.5 36 13065.5 37 14307.0 38 14551.0 39 14346.5 40 13691.5 41 13233.0 42 12505.5 43 12610.0 44 13387.5 45 13481.0 46 12861.0 47 13154.0 48 18222.0 49 17671.5 50 12417.0 51 10584.5 52 8832.0 53 8231.5 54 7778.5 55 7198.5 56 6537.0 57 5955.5 58 5455.0 59 4973.5 60 4443.0 61 3713.0 62 2879.5 63 2144.5 64 1515.0 65 1142.5 66 879.5 67 657.0 68 447.0 69 278.0 70 194.5 71 147.5 72 127.5 73 118.0 74 89.5 75 70.0 76 61.0 77 31.5 78 17.0 79 11.0 80 5.5 81 5.0 82 4.5 83 2.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05652476145348013 2 0.0 3 0.0 4 0.0 5 0.01876141018455936 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004570087096238819 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.013272495051077E-4 24-25 0.0 26-27 0.0010823890491091937 28-29 0.002886370797624517 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01491291578772667 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.4053089980204307E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.607963497030646E-4 84-85 7.215926994061292E-4 86-87 0.0 88-89 0.0 90-91 0.010703625041190917 92-93 0.0010823890491091937 94-95 0.0 96-97 0.0 98-99 4.8106179960408614E-4 100-101 0.014431853988122585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 415747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.38125418772792 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52202707651143 35.49187588795875 2 12.2627035318082 11.3751913907509 3 4.254667599471152 5.9201045824608505 4 2.0310963522694063 3.7681918477749723 5 1.0918689222345634 2.5321125010920906 6 0.742333758280997 2.065822244097738 7 0.5210908461802266 1.6918192894108322 8 0.3451831118916594 1.2808020523166386 9 0.2876914339090933 1.2009140571392625 >10 1.7140426105384217 15.28200239643755 >50 0.14563508451568785 4.614933411480629 >100 0.0722974169560006 6.036082011952688 >500 0.004161002414733848 1.2086155980929207 >1k 0.004161002414733848 3.9059271753937086 >5k 0.001040250603683462 3.62560555364042 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8173 1.965859044082098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6856 1.6490798490428074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4934 1.1867794596232806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2797 0.6727649267463145 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1766 0.4247775690504081 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1690 0.4064972206654528 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1357 0.32640043103137245 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1313 0.31581707144008253 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1216 0.2924855741592844 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1118 0.26891354597868417 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 0.19915958503609169 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.1710174697592526 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 635 0.15273712137429735 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 625 0.1503318123762769 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 571 0.1373431437869666 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 569 0.1368620819873625 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 537 0.12916509319369712 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 534 0.128443500494291 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 481 0.11569536280478271 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 478 0.11497377010537659 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 444 0.10679571951210712 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 441 0.106074126812701 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 438 0.10535253411329487 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4053089980204307E-4 0.0 18-19 0.0 0.0 0.0 2.4053089980204307E-4 0.0 20-21 0.0 0.0 0.0 2.4053089980204307E-4 0.0 22-23 0.0 0.0 0.0 7.215926994061292E-4 0.0 24-25 0.0 0.0 0.0 0.002044512648317366 0.0 26-27 0.0 0.0 0.0 0.004089025296634732 0.0 28-29 0.0 0.0 0.0 0.0078172542435664 0.0 30-31 0.0 0.0 0.0 0.014672384887924626 0.0 32-33 0.0 0.0 0.0 0.023331497280798176 0.0 34-35 0.0 0.0 0.0 0.031870344223770705 0.0 36-37 0.0 0.0 0.0 0.04546034006258614 0.0 38-39 0.0 0.0 0.0 0.06289883029823426 0.0 40-41 0.0 0.0 0.0 0.08117917868318954 0.0 42-43 0.0 0.0 0.0 0.10174457061626421 0.0 44-45 0.0 0.0 0.0 0.11292925745705923 0.0 46-47 0.0 0.0 0.0 0.12976642044320225 0.0 48-49 0.0 0.0 0.0 0.14504013258063198 0.0 50-51 0.0 0.0 0.0 0.15826933206974436 0.0 52-53 0.0 0.0 0.0 0.18737357094579155 0.0 54-55 0.0 0.0 0.0 0.2418538197509543 0.0 56-57 0.0 0.0 0.0 0.33301503077592864 0.0 58-59 0.0 0.0 0.0 0.44955225173001845 0.0 60-61 0.0 0.0 0.0 0.6179238815914486 0.0 62-63 0.0 0.0 0.0 0.7130538524631567 0.0 64-65 0.0 0.0 0.0 0.8161213430283321 0.0 66-67 0.0 0.0 0.0 1.0123945572667994 0.0 68-69 0.0 0.0 0.0 1.2601413840629037 0.0 70-71 0.0 0.0 0.0 1.5645332377623893 0.0 72-73 0.0 0.0 0.0 1.9268930383141671 0.0 74-75 0.0 0.0 0.0 2.2744601885281193 0.0 76-77 0.0 0.0 0.0 2.575364344180475 0.0 78-79 0.0 0.0 0.0 2.8345363887171766 0.0 80-81 0.0 0.0 0.0 3.0411524316471317 0.0 82-83 0.0 0.0 0.0 3.3662299427295928 0.0 84-85 0.0 0.0 0.0 3.9673166613348982 0.0 86-87 0.0 0.0 0.0 4.711759796222221 0.0 88-89 0.0 0.0 0.0 5.501783536622032 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 60 8.280751E-5 39.584034 5 GGACAAT 190 0.0 32.496666 6 TAGGACA 245 0.0 31.020958 4 TTGCGGG 40 4.495283E-4 29.684454 50-51 CTAGGAC 480 0.0 28.698425 3 TAGTACA 70 0.008384789 27.14334 4 TGGGATA 245 0.0 27.140074 6 GACAATG 235 5.456968E-12 26.273901 7 GGTAAGG 95 0.0012216778 25.012478 1 GGTATCA 5940 0.0 24.961948 1 GTATCAA 7940 0.0 24.71951 1 GAGTACT 5040 0.0 24.689928 12-13 ATGGGAT 980 0.0 24.235123 5 TACTGGC 100 0.0016528628 23.747564 7 AGTACTT 5200 0.0 23.382217 12-13 GTACTTT 5325 0.0 23.3239 14-15 CAATGGG 230 3.614332E-9 22.715061 9 GTACATG 3940 0.0 22.193813 1 GTGAGCA 130 2.9391758E-4 21.92083 9 ATGGGAG 805 0.0 21.832685 5 >>END_MODULE