FastQCFastQC Report
Thu 26 May 2016
SRR937328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453473
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66181.459403316184205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51691.1398694078809544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.8384181638157068No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18660.41149087156236425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18000.39693653205372753No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16410.3618738050556483No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13330.2939535540153438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.23507463509404086No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.2304437088867474No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9270.20442231400766972No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.16539022168905315No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7430.16384657961995533No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6810.15017432129366026No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6800.14995380099807484No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6410.1413535094702441No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.11930147991170367No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.11400899281765398No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.10915754631477509No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG4930.10871650572360426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC704.294796E-640.7275057
CGACCGT354.3159343E-640.70518-19
CATTCCG756.908076E-638.0039339
TCGACCG401.07903925E-535.6168818-19
GGGATCG550.002573877634.556677
TAGACTC702.0487927E-433.9358375
CCGGAAC600.00394003731.680453
CGATCCG300.003954923231.65944970-71
TCTAGAT1252.1408414E-730.413233
TAGTCAA1004.9430706E-528.502959
GTTCGAC504.9596638E-528.49350216-17
AGATAGT1703.616151E-927.9502496
GTACATG40000.027.5711381
CGTCTCA700.008377532527.148678
CTAGTAC908.916436E-426.4003753
TACATGG41600.026.162112
ACATGGG40450.025.845613
ATGGGAG7800.025.5824015
ATATAGG2251.0186341E-1025.3471642
CATGGGG8450.025.306874
Produced by FastQC (version 0.11.5)