##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937328_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 453473 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00106731823063 33.0 31.0 34.0 31.0 34.0 2 32.085301660738345 33.0 31.0 34.0 30.0 34.0 3 32.094695825330284 34.0 31.0 34.0 30.0 34.0 4 35.72563967424742 37.0 35.0 37.0 35.0 37.0 5 35.750615802925424 37.0 35.0 37.0 35.0 37.0 6 35.819973846292946 37.0 35.0 37.0 35.0 37.0 7 35.71929310014047 37.0 35.0 37.0 35.0 37.0 8 35.74020283456788 37.0 35.0 37.0 35.0 37.0 9 37.38126635984943 39.0 37.0 39.0 35.0 39.0 10-11 37.29577064125097 39.0 37.0 39.0 35.0 39.0 12-13 37.15919470398458 39.0 37.0 39.0 34.0 39.0 14-15 38.281373973753674 40.0 38.0 41.0 34.0 41.0 16-17 38.25548048064604 40.0 38.0 41.0 34.0 41.0 18-19 38.33882392115959 40.0 38.0 41.0 34.0 41.0 20-21 38.29428212925576 40.0 38.0 41.0 34.0 41.0 22-23 38.15363097692696 40.0 38.0 41.0 34.0 41.0 24-25 38.08464561285898 40.0 38.0 41.0 34.0 41.0 26-27 38.05871132349666 40.0 38.0 41.0 34.0 41.0 28-29 37.95363119744725 40.0 38.0 41.0 34.0 41.0 30-31 37.65400255362502 40.0 37.0 41.0 33.0 41.0 32-33 37.32363558580114 39.0 36.0 41.0 33.0 41.0 34-35 37.27443309745013 39.5 36.0 41.0 33.0 41.0 36-37 37.11267263982641 39.5 35.0 41.0 33.0 41.0 38-39 36.94903665708874 39.0 35.0 41.0 32.5 41.0 40-41 36.688075144495926 39.0 35.0 41.0 31.5 41.0 42-43 36.35833114650707 39.0 35.0 41.0 31.0 41.0 44-45 35.98586464905297 39.0 35.0 41.0 30.0 41.0 46-47 35.43579772114327 39.0 35.0 41.0 27.0 41.0 48-49 34.95077766482238 39.0 35.0 40.0 24.5 41.0 50-51 33.930573595340846 38.0 34.0 39.5 22.5 40.5 52-53 34.01530190331067 38.0 34.0 39.5 23.0 40.5 54-55 34.36602840742448 38.0 35.0 40.0 22.5 41.0 56-57 34.16546519858956 38.0 34.5 40.0 21.0 41.0 58-59 33.918630216131945 38.0 34.0 40.0 19.5 41.0 60-61 33.605984259261305 38.0 34.0 40.0 17.0 41.0 62-63 33.26597063110704 37.0 33.5 40.0 13.0 41.0 64-65 32.808835366162924 36.5 33.0 40.0 8.0 41.0 66-67 32.44690643103338 36.0 33.0 39.0 4.5 41.0 68-69 31.978322854943954 35.5 32.0 39.0 2.0 40.5 70-71 31.49970670800687 35.0 31.5 38.5 2.0 40.0 72-73 31.014699882903724 35.0 31.0 38.0 2.0 40.0 74-75 30.515901718514662 35.0 31.0 37.0 2.0 39.0 76-77 30.005784247353205 35.0 30.0 36.5 2.0 39.0 78-79 29.506393985970497 34.5 29.5 36.0 2.0 39.0 80-81 29.01947304470167 34.0 29.0 36.0 2.0 37.5 82-83 28.542698242232724 34.0 29.0 35.0 2.0 37.0 84-85 28.006868104606006 34.0 27.5 35.0 2.0 36.5 86-87 27.506717048203534 34.0 26.5 35.0 2.0 36.0 88-89 27.01759531438476 33.5 25.5 35.0 2.0 36.0 90-91 26.496225795141058 33.0 25.0 35.0 2.0 35.0 92-93 26.00930264866927 33.0 24.0 35.0 2.0 35.0 94-95 25.514884017350536 32.5 21.5 35.0 2.0 35.0 96-97 24.796536949278128 32.0 12.5 35.0 2.0 35.0 98-99 23.915948689337625 32.0 2.0 34.0 2.0 35.0 100-101 21.51312206018881 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.16197142274972265 1101 2 -0.31822581228320246 1101 3 -0.21558596312728895 1101 4 -0.043840109087504686 1101 5 -0.18850580234115455 1101 6 -0.4194486421436423 1101 7 -0.16981912692345702 1101 8 -0.3472119872942656 1101 9 -0.4173696292911657 1101 10-11 -0.5282316335336148 1101 12-13 -0.35542432672045265 1101 14-15 -0.5559909531220342 1101 16-17 -0.6147834962374574 1101 18-19 -0.19036380110231477 1101 20-21 -0.11267433066394261 1101 22-23 -0.033881808891145226 1101 24-25 -0.18596826861821825 1101 26-27 -0.24333543827034276 1101 28-29 -0.4048352478079167 1101 30-31 -0.23489944937319507 1101 32-33 -0.451125448801335 1101 34-35 -0.3317513228826101 1101 36-37 -0.37546235864226674 1101 38-39 -0.5636825306283413 1101 40-41 -0.7029526282417251 1101 42-43 -0.6647237971415052 1101 44-45 -0.3899937015628865 1101 46-47 -0.21770174149103383 1101 48-49 -0.14719270752807745 1101 50-51 -0.36656567081849545 1101 52-53 -0.20916481941097942 1101 54-55 -0.3244023320341469 1101 56-57 -0.11440018081518133 1101 58-59 0.045738663857456174 1101 60-61 -0.5023347320076326 1101 62-63 -0.45587014058131814 1101 64-65 -0.29933775222238523 1101 66-67 -0.12837638549712693 1101 68-69 0.07893776555556187 1101 70-71 -0.2927559401601627 1101 72-73 -0.08394902403653859 1101 74-75 -0.30972201898239504 1101 76-77 -0.15818224066846298 1101 78-79 0.024549399050002307 1101 80-81 7.241868522100958E-5 1101 82-83 -0.24799724583630578 1101 84-85 -0.06200654869665101 1101 86-87 -0.25569649735106026 1101 88-89 -0.2911619191668713 1101 90-91 -0.04786276304192327 1101 92-93 -0.17971486297194872 1101 94-95 -0.3081171373990266 1101 96-97 0.04947227324414882 1101 98-99 0.0725693406441259 1101 100-101 0.3557866354450283 1102 1 -0.08360514048722933 1102 2 -0.17746798691252508 1102 3 -0.14022627065062565 1102 4 -0.15208832435384068 1102 5 -0.001701848469650713 1102 6 -0.1546820303918608 1102 7 -0.09282297096518732 1102 8 -0.1705288461630161 1102 9 -0.13424771825327042 1102 10-11 -0.05890022222114766 1102 12-13 -0.08815085060842165 1102 14-15 -0.13630671369314484 1102 16-17 -0.08106026724239968 1102 18-19 -0.21973365283212587 1102 20-21 -0.3766789984289076 1102 22-23 -0.23549493354792617 1102 24-25 -0.06766651134199009 1102 26-27 -0.4181583377761058 1102 28-29 -0.313855017165416 1102 30-31 -0.2672388233435399 1102 32-33 -0.284022208823302 1102 34-35 -0.030872684771679815 1102 36-37 0.0077638906712920175 1102 38-39 -0.05349582002977371 1102 40-41 -0.1282024909545214 1102 42-43 -0.09871199044189183 1102 44-45 0.39120481573529986 1102 46-47 0.3919097357192882 1102 48-49 0.2805530365685769 1102 50-51 0.3501655208344445 1102 52-53 0.2906608258388914 1102 54-55 0.22320063336947982 1102 56-57 0.21574122500579307 1102 58-59 0.033204342056251335 1102 60-61 0.384973065905605 1102 62-63 0.4677858176833709 1102 64-65 0.12222732191270325 1102 66-67 0.11353602526619255 1102 68-69 -0.04072176217645662 1102 70-71 0.03945850245378679 1102 72-73 0.043225605288007785 1102 74-75 -0.10854272189288139 1102 76-77 -0.03793649657181675 1102 78-79 0.0872649399615888 1102 80-81 0.32633957958582727 1102 82-83 0.2896112110555116 1102 84-85 0.25309778957902296 1102 86-87 0.311421844219506 1102 88-89 -0.02775170499882762 1102 90-91 0.05827672075818313 1102 92-93 0.2508933193454581 1102 94-95 -0.07092191499375744 1102 96-97 0.09686518922437415 1102 98-99 0.195772251132869 1102 100-101 0.0018684586191071162 1103 1 0.0609131639443703 1103 2 0.06438172407013099 1103 3 0.08333827655553705 1103 4 0.101630365434211 1103 5 0.07030200509489504 1103 6 0.006281360744942788 1103 7 0.014710747724599571 1103 8 0.13224572031096926 1103 9 0.0940085437891156 1103 10-11 0.028387831728757362 1103 12-13 -0.040935050993788025 1103 14-15 -0.2597267522287936 1103 16-17 0.02493202562850172 1103 18-19 0.050145923275771054 1103 20-21 0.0040483618793771825 1103 22-23 0.024837436394271606 1103 24-25 -0.04921275411656012 1103 26-27 -0.42490689268939974 1103 28-29 -0.07267004606715233 1103 30-31 0.004842101511954411 1103 32-33 -0.2002168138329381 1103 34-35 -0.030101971862237065 1103 36-37 -0.2915521016215763 1103 38-39 -0.5408753961376789 1103 40-41 -0.38384796301617996 1103 42-43 -0.47798463013360504 1103 44-45 -0.6650331418754902 1103 46-47 -0.2550025956872659 1103 48-49 -0.2619806821212123 1103 50-51 -0.32860615546614014 1103 52-53 -0.21582761346747503 1103 54-55 0.10139909194365515 1103 56-57 -0.009812724897862779 1103 58-59 0.17130646151674966 1103 60-61 0.07001641850676066 1103 62-63 0.06629737837701555 1103 64-65 0.3069479384830345 1103 66-67 0.4552942140908556 1103 68-69 0.43731291990446763 1103 70-71 0.38476197066187723 1103 72-73 0.341269921280297 1103 74-75 0.4178493782997954 1103 76-77 0.44186600824514244 1103 78-79 0.18746725210031912 1103 80-81 0.243646901358467 1103 82-83 0.0903530222308504 1103 84-85 0.3823945140491567 1103 86-87 0.46088716213858305 1103 88-89 0.6772001254443296 1103 90-91 0.6769279731666629 1103 92-93 0.6235089262818754 1103 94-95 0.48183820061317917 1103 96-97 0.6507235707272656 1103 98-99 0.8171643513255482 1103 100-101 1.5681786705651568 1104 1 -0.07572333437833123 1104 2 0.012093766075523149 1104 3 -0.14738430517918033 1104 4 -0.14589974533657823 1104 5 0.13666468539489074 1104 6 0.14808025380468592 1104 7 -0.24160119141671998 1104 8 -0.044127783746020555 1104 9 0.005134751866251008 1104 10-11 0.03049884816397963 1104 12-13 0.012028432260102306 1104 14-15 -0.031771521130060876 1104 16-17 -0.1416578768041532 1104 18-19 -0.13922568470464114 1104 20-21 -0.12670223880075326 1104 22-23 -0.2580214939728904 1104 24-25 -0.13872560828754388 1104 26-27 -0.1315945927561799 1104 28-29 -0.13327732792238578 1104 30-31 -0.13839420182959117 1104 32-33 -0.2490753296732322 1104 34-35 -0.4205805200970474 1104 36-37 -0.012840358996697887 1104 38-39 0.12492383468470081 1104 40-41 0.4608944545966054 1104 42-43 0.3175595899033965 1104 44-45 0.3197406723090026 1104 46-47 0.0024243439530167166 1104 48-49 0.18888636990190832 1104 50-51 -0.3046518762439163 1104 52-53 -0.3786253627401308 1104 54-55 -0.375345183363585 1104 56-57 -0.14572955852673886 1104 58-59 -0.1799762688335278 1104 60-61 -0.2816770004954634 1104 62-63 -0.0038708888239327166 1104 64-65 0.240673537052146 1104 66-67 -0.1687913319715264 1104 68-69 0.06910559506125935 1104 70-71 0.4820049831974771 1104 72-73 0.4679865614633023 1104 74-75 0.3319386858096429 1104 76-77 0.28945062162208046 1104 78-79 0.5393698536402063 1104 80-81 0.6542645463852992 1104 82-83 0.23103883389747892 1104 84-85 0.571338161424972 1104 86-87 0.451604447141154 1104 88-89 0.12655108384578995 1104 90-91 0.12993674731861304 1104 92-93 0.1329816327584723 1104 94-95 -0.029716735710888287 1104 96-97 -0.3638021414529149 1104 98-99 0.05628685936660105 1104 100-101 -0.09194960246987094 1105 1 0.10278800320350712 1105 2 -0.009940634614935107 1105 3 -0.06636047925748301 1105 4 0.026700880606036037 1105 5 0.19766357822619085 1105 6 0.05601089448049379 1105 7 0.04451473873575651 1105 8 0.05431433399744634 1105 9 0.27908926205307694 1105 10-11 0.058147553125316165 1105 12-13 0.1536762828707836 1105 14-15 0.031092994549013042 1105 16-17 -0.08208871363189729 1105 18-19 0.1499654718943475 1105 20-21 0.11210838304446469 1105 22-23 0.39416407447541246 1105 24-25 0.077444721334885 1105 26-27 0.018914966381444742 1105 28-29 0.039591372331308605 1105 30-31 0.2855620519299933 1105 32-33 0.355624034794495 1105 34-35 0.2554876361619449 1105 36-37 0.28157497746924776 1105 38-39 0.30387106545526876 1105 40-41 0.3239346352307422 1105 42-43 0.4965961787776507 1105 44-45 0.5097770258009504 1105 46-47 0.4933119968425572 1105 48-49 0.394575647801247 1105 50-51 0.24309586364990565 1105 52-53 0.36939350271708093 1105 54-55 0.44388991126881905 1105 56-57 0.35300417175999144 1105 58-59 0.4967893165274404 1105 60-61 0.8784723365110025 1105 62-63 0.917382827165504 1105 64-65 1.0524461402934548 1105 66-67 1.0392125387599904 1105 68-69 0.8939244814413421 1105 70-71 0.8069001508855891 1105 72-73 1.149472870058247 1105 74-75 1.017297905386478 1105 76-77 0.9962775807440138 1105 78-79 1.0800038166939032 1105 80-81 0.909120396507781 1105 82-83 1.245676127175134 1105 84-85 1.158965769155973 1105 86-87 1.3237504364879236 1105 88-89 1.2799288201652814 1105 90-91 1.5639076470893336 1105 92-93 1.8761577248888557 1105 94-95 1.6104730521834867 1105 96-97 1.534737180471641 1105 98-99 1.2211351249476046 1105 100-101 1.5228276125213682 1106 1 -0.10527180715389406 1106 2 0.03243397387178959 1106 3 0.11457765091800098 1106 4 -0.11591185376560986 1106 5 -0.2569959661167118 1106 6 -0.19076046176440542 1106 7 -0.4802739513573471 1106 8 -0.4119994343983109 1106 9 -0.5340516398218966 1106 10-11 -0.9506159084956565 1106 12-13 -0.42967045845156093 1106 14-15 -0.40900279212451807 1106 16-17 -0.2199818358698451 1106 18-19 -0.6213022802831105 1106 20-21 -0.4744240964681268 1106 22-23 -0.479122384528317 1106 24-25 -0.5628135701655239 1106 26-27 -0.4685995142466979 1106 28-29 -0.012139330890903466 1106 30-31 -0.39169960765725875 1106 32-33 -0.2697084833331047 1106 34-35 -0.00440209653638135 1106 36-37 -0.3342459132502711 1106 38-39 0.12689633683297075 1106 40-41 0.05498378355527933 1106 42-43 -0.19420218652032872 1106 44-45 0.46718520789216456 1106 46-47 0.16171365728791187 1106 48-49 0.24158244833327558 1106 50-51 0.041243952206990286 1106 52-53 -0.010221527102288519 1106 54-55 -0.04366211172855827 1106 56-57 0.12260397010383173 1106 58-59 -0.1748838932378618 1106 60-61 -0.21747791448655818 1106 62-63 0.323374052977492 1106 64-65 0.3350704591676106 1106 66-67 0.3314772017367815 1106 68-69 0.12520961037256484 1106 70-71 0.4850467377479042 1106 72-73 0.07244129156251589 1106 74-75 0.6250847290875079 1106 76-77 0.768583111271326 1106 78-79 0.896480626236098 1106 80-81 0.5864866384093581 1106 82-83 0.3894151326241406 1106 84-85 0.061578181735882254 1106 86-87 0.4028924324548022 1106 88-89 0.36156202049136255 1106 90-91 0.29126691683661576 1106 92-93 0.2861874369925168 1106 94-95 0.6343231830454599 1106 96-97 0.49755146373418313 1106 98-99 0.20944872172110607 1106 100-101 0.47804492920734276 1107 1 0.12162588261508489 1107 2 -0.07766600671450874 1107 3 0.07713346532297294 1107 4 -0.08472858838024422 1107 5 -0.034078086093416005 1107 6 -0.03841140332915671 1107 7 0.030015312863191923 1107 8 -0.017294522730672668 1107 9 0.02897010352890561 1107 10-11 0.03950736853793302 1107 12-13 -0.014933028826241923 1107 14-15 -0.04360044306608302 1107 16-17 0.012157554539776072 1107 18-19 0.24766733726688273 1107 20-21 0.2111345329242269 1107 22-23 -0.09593222727729511 1107 24-25 0.2854440497141084 1107 26-27 0.20736146420409085 1107 28-29 0.07550141847814729 1107 30-31 0.23612751329012838 1107 32-33 0.5305487482724089 1107 34-35 0.1825761601128093 1107 36-37 -0.18452983870164275 1107 38-39 -0.23633410385815523 1107 40-41 0.005345363830954852 1107 42-43 0.029844115168671692 1107 44-45 -0.04629518426470014 1107 46-47 0.05764405915163451 1107 48-49 0.0069556768326037854 1107 50-51 0.06690644492685038 1107 52-53 -0.024842474491144628 1107 54-55 0.349586771983347 1107 56-57 0.21842274315761046 1107 58-59 -0.011432621640118157 1107 60-61 0.6119120097999868 1107 62-63 0.80059934903651 1107 64-65 0.7334154407245848 1107 66-67 0.5418611077744444 1107 68-69 0.4809779077905354 1107 70-71 0.5303413407376141 1107 72-73 0.5468394666741467 1107 74-75 0.5863665190312126 1107 76-77 0.4538869357714219 1107 78-79 0.8147731907866707 1107 80-81 1.158608556483518 1107 82-83 1.0945452044548531 1107 84-85 0.9561010899090547 1107 86-87 1.019944946529737 1107 88-89 1.2294760177734432 1107 90-91 1.4244813412202326 1107 92-93 1.0296474447579982 1107 94-95 1.360686995897332 1107 96-97 1.2701077634818034 1107 98-99 1.4555659805058063 1107 100-101 1.2093107028435277 1108 1 -0.03193847382056347 1108 2 0.06503201308747464 1108 3 0.22894039601604277 1108 4 0.05957834231282533 1108 5 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-3.2213204996706573 2316 76-77 -3.120714274349595 2316 78-79 -2.875373948927301 2316 80-81 -2.0311604204141744 2316 82-83 -2.1099721222778207 2316 84-85 -1.4900966548654253 2316 86-87 -1.8638559335582698 2316 88-89 -1.7873350383321664 2316 90-91 -2.0661677236862594 2316 92-93 -2.1296622362100983 2316 94-95 -2.308283026104867 2316 96-97 -1.4056348107756236 2316 98-99 -0.34283885997824015 2316 100-101 0.0993684586191037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1720.0 3 318.0 4 220.0 5 221.0 6 250.0 7 330.0 8 360.0 9 544.0 10 620.0 11 985.0 12 1573.0 13 2344.0 14 2777.0 15 3725.0 16 5313.0 17 7952.0 18 6479.0 19 3577.0 20 3291.0 21 3371.0 22 3658.0 23 3970.0 24 4410.0 25 4848.0 26 5567.0 27 6152.0 28 7222.0 29 8686.0 30 10513.0 31 13295.0 32 16325.0 33 21831.0 34 30207.0 35 45322.0 36 61850.0 37 85187.0 38 69721.0 39 8739.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06427822949689 20.196044620710694 15.92409350364544 19.815583646146976 2 14.371215135039561 22.19378508597445 41.130486672855056 22.30451310613093 3 17.782477635161413 29.17661150595806 31.463261553693293 21.577649305187226 4 11.160543595251829 18.127429724467312 39.07494215073298 31.63708452954788 5 11.368332651911112 40.08066565742511 33.91272638872007 14.63827530194371 6 26.653594568963424 39.07547699524497 18.616907218318033 15.654021217473577 7 25.30169488527968 36.6276049438391 21.43089258357816 16.63980758730306 8 25.803414137082708 33.591209801663524 20.772833671888296 19.832542389365468 9 27.71929049013649 16.462618113499474 21.166602199259547 34.65148919710449 10-11 23.267481033476578 28.283662263250044 27.212540010964332 21.23631669230905 12-13 25.854074767532726 26.763606782418588 28.0453959155613 19.33692253448739 14-15 23.05760090352625 26.8949352358661 28.5901520501183 21.457311810489347 16-17 18.979434166009952 30.759555370360754 29.13303409880929 21.12797636482001 18-19 18.863877232786695 28.221716561660443 33.90387758642983 19.010528619123033 20-21 20.40972392590579 27.03906058335599 33.600986626176095 18.95022886456213 22-23 19.838309007487823 27.03108286110841 33.518171450798725 19.612436680605036 24-25 19.50733048394012 27.448319756726058 33.848957113243486 19.195392646090333 26-27 19.28881727773744 27.45679847317952 33.801347571575235 19.4530366775078 28-29 18.7683465959328 27.168213969938108 34.756410256410255 19.30702917771883 30-31 18.806056258793543 28.143433287318615 34.094031386531356 18.956479067356483 32-33 18.539865407760992 28.503537218434428 33.34556430266344 19.611033071141136 34-35 18.836675932731684 27.968742228387317 33.63404753878208 19.560534300098926 36-37 19.259309191006242 27.918345877456936 33.23870448726378 19.58364044427304 38-39 19.2181758910861 27.935266068833677 33.57169299452447 19.27486504555576 40-41 18.60161110313049 28.184801706133904 33.942230129175556 19.271357061560053 42-43 18.55084411429607 28.657782571363217 32.92106533998296 19.870307974357758 44-45 18.694149491743104 29.24303599079976 32.07030822572641 19.992506291730727 46-47 18.911695460423783 29.052845663251208 31.44410916205193 20.591349714273083 48-49 19.194105345070763 29.348700761812964 31.006738264410554 20.450455628705715 50-51 19.75312735926745 29.595093055311455 30.52116943395267 20.130610151468424 52-53 19.18523341982402 29.88284510502151 30.72969468087812 20.20222679427636 54-55 18.975949560176947 29.747469276497156 30.868305106103584 20.408276057222317 56-57 19.38528453299062 30.29143400825912 29.74752994400728 20.575751514742983 58-59 19.22412307257163 29.459657139826668 30.53888444489621 20.777335342705495 60-61 19.55228811246604 29.463004272498566 30.66133681585608 20.323370799179308 62-63 20.028683593227754 29.44079147270805 30.697411294662047 19.833113639402153 64-65 19.80307518254546 29.197440772850356 31.07554051023238 19.923943534371798 66-67 19.255373451272682 29.740757354452224 30.717889112560187 20.285980081714907 68-69 19.048776553312134 30.08874490921104 30.646494288814296 20.215984248662526 70-71 19.21591721422234 29.898473399677155 30.367822619582956 20.517786766517546 72-73 19.027141221863246 29.765403670981744 29.9710290328426 21.23642607431241 74-75 18.98492626665989 30.029899957790896 30.15243895701522 20.832734818533996 76-77 19.134367910214376 29.329514173217035 30.82170511796188 20.714412798606713 78-79 18.794671075023096 29.255714140480997 31.089410662270094 20.860204122225806 80-81 18.714882868309306 30.42208513304317 30.16882742343429 20.69420457521323 82-83 18.84429633869173 30.014286361441627 29.763805453567116 21.377611846299523 84-85 18.683959590423775 29.262910855184288 29.853497054803523 22.19963249958841 86-87 19.155709617856 29.519444226512835 29.941335266356013 21.38351088927515 88-89 19.510914965370013 28.52255700577682 30.25726080557262 21.709267223280545 90-91 18.85867733433527 28.512291209671623 30.911522076659132 21.717509379333972 92-93 18.833451932129076 29.005250759105955 30.539042576231033 21.622254732533932 94-95 18.402012759854415 28.68591620572227 30.62254565062219 22.289525383801127 96-97 18.54005032327789 28.509672920794433 30.150538870146278 22.79973788578141 98-99 18.609701193171833 28.56860268383543 30.075953227929304 22.74574289506343 100-101 18.84599831619699 28.183812952024873 30.945956994522117 22.02423173725602 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1155.0 1 804.5 2 524.5 3 655.0 4 628.0 5 635.5 6 1139.5 7 2349.0 8 3614.0 9 3949.0 10 3214.0 11 2366.5 12 2158.5 13 2147.5 14 2119.5 15 2045.5 16 1932.5 17 1916.0 18 1879.5 19 1877.5 20 2000.5 21 2174.0 22 2325.0 23 2603.0 24 2873.5 25 3142.0 26 3664.0 27 4112.5 28 4419.5 29 5179.0 30 6284.5 31 7264.5 32 8600.5 33 10382.0 34 11864.5 35 12774.5 36 14571.0 37 16297.5 38 16494.0 39 15863.5 40 15022.5 41 14535.0 42 13896.0 43 14183.5 44 14780.5 45 14918.0 46 14662.0 47 14833.5 48 19559.0 49 19154.0 50 13756.0 51 11559.5 52 9837.5 53 9271.5 54 8961.0 55 8224.0 56 7416.5 57 6657.5 58 6020.0 59 5431.5 60 4780.0 61 4150.0 62 3417.5 63 2461.0 64 1658.5 65 1273.0 66 953.5 67 742.5 68 549.5 69 355.5 70 228.0 71 168.5 72 155.0 73 145.5 74 109.0 75 74.5 76 52.0 77 33.5 78 20.0 79 13.5 80 10.5 81 5.0 82 1.0 83 1.5 84 2.5 85 2.5 86 1.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24852637312475054 2 0.22338705942801443 3 0.2154483287869399 4 0.22140237676774582 5 0.21787405203837934 6 0.24477752809979866 7 0.24521856869096947 8 0.23375151332052846 9 0.2311052697735036 10-11 0.24191076425718838 12-13 0.2420210244049811 14-15 0.22570252253166118 16-17 0.24180050410939571 18-19 0.22923084726102766 20-21 0.2253717420882831 22-23 0.22162289706333121 24-25 0.2158893693781107 26-27 0.22702564430517364 28-29 0.23661827716313868 30-31 0.23849269967561462 32-33 0.2358464561285898 34-35 0.24378518676966432 36-37 0.22129211661995313 38-39 0.22173315721112394 40-41 0.21787405203837934 42-43 0.22415888046256335 44-45 0.24113894322263948 46-47 0.24654169046448188 48-49 0.24940845430709216 50-51 0.2501802753416411 52-53 0.26649877721496096 54-55 0.24962897460267755 56-57 0.22129211661995313 58-59 0.20442231400766972 60-61 0.2045325741554624 62-63 0.20938402065834127 64-65 0.20166581031285216 66-67 0.20376075312091346 68-69 0.20728907785027995 70-71 0.205414655337804 72-73 0.20971480110171942 74-75 0.21258156494432964 76-77 0.22437940075814877 78-79 0.2202997752898188 80-81 0.21379442657004938 82-83 0.20806089888482887 84-85 0.2109276627274391 86-87 0.21754327159500125 88-89 0.21500728819576911 90-91 0.22393836016697796 92-93 0.21324312583108584 94-95 0.21169948376198802 96-97 0.22018951514202612 98-99 0.22570252253166118 100-101 0.2042017937120843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 453473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.420811839236464 #Duplication Level Percentage of deduplicated Percentage of total 1 76.71779634458741 37.1473798152213 2 12.268798094960493 11.881303280993295 3 4.2630149311403 6.192559315458002 4 2.0066351047236086 3.8865160334331366 5 1.1258278679289973 2.725674967817936 6 0.6641469115944661 1.9295119583955394 7 0.47620013707704745 1.6140598064658422 8 0.33585924117134547 1.3010061696981168 9 0.26718250829631623 1.1643474564856047 >10 1.6659958435277173 15.507920001308717 >50 0.13204688722248512 4.4194507375716805 >100 0.0687554481741011 5.655662275865918 >500 0.0036426727509457544 1.208211027278389 >1k 0.003187338657077535 2.7676410630338713 >5k 9.106681877364386E-4 2.5987560909726954 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6618 1.459403316184205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5169 1.1398694078809544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3802 0.8384181638157068 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1866 0.41149087156236425 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1800 0.39693653205372753 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1641 0.3618738050556483 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1333 0.2939535540153438 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1066 0.23507463509404086 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1045 0.2304437088867474 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 927 0.20442231400766972 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.16539022168905315 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 743 0.16384657961995533 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 681 0.15017432129366026 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 680 0.14995380099807484 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 641 0.1413535094702441 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.11930147991170367 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.11400899281765398 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 495 0.10915754631477509 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 493 0.10871650572360426 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.102601477927021E-4 0.0 18-19 0.0 0.0 0.0 3.307804433781063E-4 0.0 20-21 0.0 0.0 0.0 6.615608867562126E-4 0.0 22-23 0.0 0.0 0.0 0.0017641623646832336 0.0 24-25 0.0 0.0 0.0 0.0027565036948175524 0.0 26-27 0.0 0.0 0.0 0.003418064581573765 0.0 28-29 0.0 0.0 0.0 0.0052924870940497006 0.0 30-31 0.0 0.0 0.0 0.012790177143953444 0.0 32-33 0.0 0.0 0.0 0.021280208523991505 0.0 34-35 0.0 0.0 0.0 0.03462168640690846 0.0 36-37 0.0 0.0 0.0 0.05038888754126486 0.0 38-39 0.0 0.0 0.0 0.07166909606525637 0.0 40-41 0.0 0.0 0.0 0.09570580828406541 0.0 42-43 0.0 0.0 0.0 0.11356795222648317 0.0 44-45 0.0 0.0 0.0 0.12613760907485122 0.0 46-47 0.0 0.0 0.0 0.14829989878118433 0.0 48-49 0.0 0.0 0.0 0.16969036745296853 0.0 50-51 0.0 0.0 0.0 0.1878832918387644 0.0 52-53 0.0 0.0 0.0 0.22724616460075903 0.0 54-55 0.0 0.0 0.0 0.2945048547543073 0.0 56-57 0.0 0.0 0.0 0.39539288998462974 0.0 58-59 0.0 0.0 0.0 0.5305718311784825 0.0 60-61 0.0 0.0 0.0 0.6949697115374014 0.0 62-63 0.0 0.0 0.0 0.7992758113492975 0.0 64-65 0.0 0.0 0.0 0.9203414536256844 0.0 66-67 0.0 0.0 0.0 1.1227790849730854 0.0 68-69 0.0 0.0 0.0 1.3972165928291211 0.0 70-71 0.0 0.0 0.0 1.6958010730517583 0.0 72-73 0.0 0.0 0.0 2.0348510275143172 0.0 74-75 0.0 0.0 0.0 2.383162834391463 0.0 76-77 0.0 0.0 0.0 2.7095328718578617 0.0 78-79 0.0 0.0 0.0 3.012196977548829 0.0 80-81 0.0 0.0 0.0 3.238009760228283 0.0 82-83 0.0 0.0 0.0 3.567467081832876 0.0 84-85 0.0 0.0 0.0 4.146112337448977 0.0 86-87 0.0 0.0 0.0 4.863905899579468 0.0 88-89 0.0 0.0 0.0 5.595923020775217 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGTC 70 4.294796E-6 40.727505 7 CGACCGT 35 4.3159343E-6 40.705 18-19 CATTCCG 75 6.908076E-6 38.003933 9 TCGACCG 40 1.07903925E-5 35.61688 18-19 GGGATCG 55 0.0025738776 34.55667 7 TAGACTC 70 2.0487927E-4 33.935837 5 CCGGAAC 60 0.003940037 31.68045 3 CGATCCG 30 0.0039549232 31.659449 70-71 TCTAGAT 125 2.1408414E-7 30.41323 3 TAGTCAA 100 4.9430706E-5 28.50295 9 GTTCGAC 50 4.9596638E-5 28.493502 16-17 AGATAGT 170 3.616151E-9 27.950249 6 GTACATG 4000 0.0 27.571138 1 CGTCTCA 70 0.0083775325 27.14867 8 CTAGTAC 90 8.916436E-4 26.400375 3 TACATGG 4160 0.0 26.16211 2 ACATGGG 4045 0.0 25.84561 3 ATGGGAG 780 0.0 25.582401 5 ATATAGG 225 1.0186341E-10 25.347164 2 CATGGGG 845 0.0 25.30687 4 >>END_MODULE