FastQCFastQC Report
Thu 26 May 2016
SRR937328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453473
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88381.9489583723838022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73161.6133264825028173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51421.1339153599001486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27680.6104001781803988No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19810.43685070555468575No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19450.42891197491361116No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.31049257618424914No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.29064574958156275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.2807223362802196No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11640.2566856240614105No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.16539022168905315No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG7490.16516970139346776No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7140.1574514910479786No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.1475280777466354No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.14488183419961057No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6570.14488183419961057No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6280.13848674562763386No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG5380.11863991902494747No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5120.11290639133972695No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG5100.11246535074855614No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5080.11202431015738534No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4910.10827546513243345No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA1600.041.5602345
CTGGTCG3050.035.8216679
GTTAGCC352.0536501E-433.9267264-65
GGGCGCG851.6294112E-533.531287
GGTATAT953.4665794E-530.0248491
GGGTATG804.4879326E-429.6891547
GTATGGT1303.0081355E-729.254981
TAAGGTG1004.9484268E-528.4984465
CCCTGGT4050.028.1497177
CATGGGT3200.026.7172934
TGGGGCG1257.3811243E-626.5985475
GCTACGG458.9489366E-426.38744772-73
ATAACCC4150.026.3238643
GAATAAC4350.025.1357461
GTTCTAG950.001219507725.0207061
GTAGGAT950.001225799224.9986363
CTAGATC2301.3460522E-1024.7812564
ACCCTGG5200.024.6621176
TAGATCA2351.7644197E-1024.2539965
TACCTGG2751.8189894E-1224.1804982
Produced by FastQC (version 0.11.5)