##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937328_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 453473 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37975579582467 33.0 31.0 34.0 31.0 34.0 2 32.547095416926695 34.0 31.0 34.0 31.0 34.0 3 32.55219164095767 34.0 31.0 34.0 31.0 34.0 4 36.19905043960721 37.0 35.0 37.0 35.0 37.0 5 36.16392155652046 37.0 35.0 37.0 35.0 37.0 6 36.247368641572926 37.0 36.0 37.0 35.0 37.0 7 36.20081460197189 37.0 36.0 37.0 35.0 37.0 8 36.19522661768176 37.0 36.0 37.0 35.0 37.0 9 37.9131723388162 39.0 38.0 39.0 35.0 39.0 10-11 37.840668573432154 39.0 38.0 39.0 35.0 39.0 12-13 37.74730358808573 39.0 37.0 39.0 35.0 39.0 14-15 38.83410699203701 40.0 38.0 41.0 35.0 41.0 16-17 38.83500340703857 40.0 38.0 41.0 35.5 41.0 18-19 38.966521711325704 40.0 38.0 41.0 35.5 41.0 20-21 38.95231799908704 40.0 38.0 41.0 35.0 41.0 22-23 38.80825760298849 40.0 38.0 41.0 35.0 41.0 24-25 38.73450348752847 40.0 38.0 41.0 35.0 41.0 26-27 38.619669748805336 40.0 38.0 41.0 35.0 41.0 28-29 38.46080031225674 40.0 38.0 41.0 34.0 41.0 30-31 38.344151691500926 40.0 37.5 41.0 35.0 41.0 32-33 38.10219020757576 40.0 36.5 41.0 34.0 41.0 34-35 37.913232981897494 40.0 36.0 41.0 34.0 41.0 36-37 37.69646373654 40.0 35.5 41.0 33.0 41.0 38-39 37.515922667942746 40.0 35.0 41.0 33.0 41.0 40-41 37.25569107752832 40.0 35.0 41.0 33.0 41.0 42-43 36.931195462574394 40.0 35.0 41.0 33.0 41.0 44-45 36.43742405832321 40.0 35.0 41.0 31.5 41.0 46-47 35.753614878945385 39.0 35.0 41.0 29.0 41.0 48-49 35.1715118650945 39.0 35.0 41.0 25.0 41.0 50-51 34.84010845188136 39.0 35.0 41.0 23.5 41.0 52-53 34.554956965464314 39.0 35.0 40.5 22.5 41.0 54-55 34.333780621999544 38.5 35.0 40.0 21.0 41.0 56-57 34.08663691112812 38.0 34.5 40.0 19.5 41.0 58-59 33.82101580468959 38.0 34.0 40.0 17.5 41.0 60-61 33.50001764162364 38.0 34.0 40.0 13.0 41.0 62-63 33.111646117850455 37.0 33.5 40.0 7.5 41.0 64-65 32.702974598267154 36.5 33.0 39.5 4.5 41.0 66-67 32.28014457310579 36.0 33.0 39.0 2.0 40.5 68-69 31.8437084457068 35.5 32.0 39.0 2.0 40.0 70-71 31.42657115197597 35.0 32.0 38.5 2.0 40.0 72-73 30.949165661461652 35.0 31.0 37.5 2.0 40.0 74-75 30.455063476767084 35.0 31.0 37.0 2.0 39.0 76-77 29.198353595473158 33.5 29.5 35.5 2.0 38.5 78-79 29.696892648514904 34.0 30.0 36.0 2.0 38.5 80-81 29.474848557687007 34.0 30.0 36.0 2.0 37.5 82-83 29.132304459140897 34.0 30.0 35.0 2.0 37.0 84-85 28.74492417409636 34.0 29.5 35.0 2.0 36.5 86-87 28.303958559826054 34.0 29.0 35.0 2.0 36.0 88-89 27.81895945293325 34.0 28.5 35.0 2.0 36.0 90-91 27.31683804768972 34.0 27.0 35.0 2.0 35.0 92-93 27.00515576451079 34.0 26.0 35.0 2.0 35.0 94-95 26.601677497888517 33.0 25.0 35.0 2.0 35.0 96-97 26.135777653796367 33.0 24.5 35.0 2.0 35.0 98-99 25.457053672434743 33.0 21.0 35.0 2.0 35.0 100-101 23.217915950894543 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.09584681948513207 1101 2 -0.05064459123820342 1101 3 0.07225916475319849 1101 4 0.03001061381550585 1101 5 -0.09155203763585718 1101 6 -0.11633157075740996 1101 7 -0.09943331626779184 1101 8 -0.004365543347262246 1101 9 -0.028757096947742866 1101 10-11 -0.09797934325293767 1101 12-13 -0.1346520901396815 1101 14-15 -0.3851560975652788 1101 16-17 -0.2367868601396026 1101 18-19 -0.25313671297084284 1101 20-21 -0.25392525516119946 1101 22-23 -0.14399055908865677 1101 24-25 -0.22651875166867796 1101 26-27 -0.2485473940436549 1101 28-29 -0.5734656433613878 1101 30-31 -0.3197792545490117 1101 32-33 -0.03599068079392964 1101 34-35 0.04010306948482878 1101 36-37 -0.0825970699891414 1101 38-39 -0.020000277066898775 1101 40-41 -0.12457681779435603 1101 42-43 -0.27404375706332473 1101 44-45 -0.26893965925094676 1101 46-47 0.16608308905879454 1101 48-49 0.5487432310348268 1101 50-51 0.5074100181268406 1101 52-53 0.2872751994577314 1101 54-55 0.35867817652837886 1101 56-57 0.35571419897010514 1101 58-59 0.24495403090255508 1101 60-61 0.19601635944216866 1101 62-63 0.06841453200482306 1101 64-65 0.12356792251895143 1101 66-67 0.12551194365317286 1101 68-69 0.17981943076929952 1101 70-71 -0.17991904671659498 1101 72-73 0.2132558139962839 1101 74-75 0.3079621741913421 1101 76-77 0.48410647040466515 1101 78-79 0.24658089608563571 1101 80-81 -0.004155379149189287 1101 82-83 0.06805495704385933 1101 84-85 0.22486558019772218 1101 86-87 0.027130622733178456 1101 88-89 0.11486745872324278 1101 90-91 0.09557878295865052 1101 92-93 0.06682953536041225 1101 94-95 0.10589191811383003 1101 96-97 0.04944634834807715 1101 98-99 0.26373904673137716 1101 100-101 0.6431122026846019 1102 1 -0.007521723384087409 1102 2 -0.11144909425851779 1102 3 -0.01036027511501203 1102 4 -0.06894600342776869 1102 5 -0.05882825949198178 1102 6 -0.18293508530985747 1102 7 -0.09979300874445585 1102 8 -0.08343748184259425 1102 9 -0.12814754175828114 1102 10-11 -0.1647599582996122 1102 12-13 -0.3072234794861899 1102 14-15 -0.17439003496231464 1102 16-17 -0.2918582494861184 1102 18-19 -0.1717569765622784 1102 20-21 -0.1943755571930481 1102 22-23 -0.22876128945658536 1102 24-25 -0.3348973897796128 1102 26-27 -0.6513288328135616 1102 28-29 -0.39260622545913293 1102 30-31 -0.22745910078734255 1102 32-33 -0.178382223902112 1102 34-35 -0.3339414115695405 1102 36-37 -0.20129970590348023 1102 38-39 -0.13243300633653377 1102 40-41 -0.42568335266530966 1102 42-43 -0.27903826557513156 1102 44-45 -0.12469062026137578 1102 46-47 -0.3079160872179756 1102 48-49 -0.18067741696078343 1102 50-51 -0.20189256287261514 1102 52-53 -0.2502742239360103 1102 54-55 -0.15080287783735713 1102 56-57 -0.2747168828530704 1102 58-59 -0.09557727058014365 1102 60-61 -0.129271943687975 1102 62-63 0.06855593782579206 1102 64-65 0.0890800037929722 1102 66-67 -0.021902938279829698 1102 68-69 -0.11302241437073235 1102 70-71 -0.06057939817184277 1102 72-73 0.12735246418848334 1102 74-75 0.3075612955532314 1102 76-77 0.33415452092635434 1102 78-79 0.3019254869697612 1102 80-81 0.13502227049386306 1102 82-83 0.09702942162375905 1102 84-85 -0.2146552874574148 1102 86-87 -0.056514352555122827 1102 88-89 0.4610453829407035 1102 90-91 0.46083688290373814 1102 92-93 0.4442883491989633 1102 94-95 0.3409907649013064 1102 96-97 0.6735292698197952 1102 98-99 0.5375364108170437 1102 100-101 1.0371360906582439 1103 1 0.12010833441937763 1103 2 0.08850273618463689 1103 3 0.10630639155165511 1103 4 0.09637191564737435 1103 5 0.03363802181822706 1103 6 0.03404950043195498 1103 7 0.16040930339427462 1103 8 -0.03794615236282084 1103 9 0.06185245824171659 1103 10-11 -0.05889772323217812 1103 12-13 -0.05848070491972379 1103 14-15 -0.06339774209141069 1103 16-17 0.033026143577465916 1103 18-19 0.11772760917953207 1103 20-21 0.030749683654725857 1103 22-23 0.08738996295189594 1103 24-25 7.336314150023782E-4 1103 26-27 -0.1346910678809934 1103 28-29 0.1411991695312338 1103 30-31 0.05773357744001828 1103 32-33 0.1617044813001982 1103 34-35 0.02398729748633599 1103 36-37 -0.003048260816775894 1103 38-39 -0.011315472617845046 1103 40-41 -0.12937314071925954 1103 42-43 0.023861541746633463 1103 44-45 -0.052768654943456283 1103 46-47 -0.08017523943377824 1103 48-49 0.00665398959027641 1103 50-51 0.023463891848003016 1103 52-53 -0.06728289445624114 1103 54-55 -0.07404950596837523 1103 56-57 -0.0028768057817742942 1103 58-59 0.10566068703064246 1103 60-61 0.08585329715980805 1103 62-63 0.2857187509279129 1103 64-65 0.17394994598950575 1103 66-67 0.21703251451399552 1103 68-69 0.2199929998874559 1103 70-71 0.29080306810946865 1103 72-73 0.42748733894763546 1103 74-75 0.5271566712757725 1103 76-77 0.7391400700593032 1103 78-79 0.6405237528657146 1103 80-81 0.5377053148098554 1103 82-83 0.56622499002453 1103 84-85 0.5494728435637803 1103 86-87 0.6306388266356286 1103 88-89 0.9779817991256756 1103 90-91 0.8957790794355311 1103 92-93 1.0676072316652423 1103 94-95 1.1367807456334873 1103 96-97 1.1403019095115106 1103 98-99 1.33076377112533 1103 100-101 1.6673239519299194 1104 1 -0.13384310452618564 1104 2 -0.12881297208056708 1104 3 -0.09422481694767981 1104 4 -0.17638292241846898 1104 5 -0.14232095537511924 1104 6 -0.1889045408211416 1104 7 -0.12779433676570306 1104 8 -0.1152170303153639 1104 9 -0.20109574892959614 1104 10-11 -0.19563977237664432 1104 12-13 -0.1209917397783542 1104 14-15 -0.3096689194244675 1104 16-17 -0.3795010117703086 1104 18-19 -0.2652529924985316 1104 20-21 -0.2082135386007593 1104 22-23 -0.37792795612325136 1104 24-25 -0.3866935385179886 1104 26-27 -0.5543899217909853 1104 28-29 -0.32299799172739085 1104 30-31 -0.2602545855150993 1104 32-33 -0.2900223301438132 1104 34-35 -0.44774619244603286 1104 36-37 -0.46073197681981526 1104 38-39 -0.4909057819009419 1104 40-41 -0.4501919848034177 1104 42-43 -0.4084074554821697 1104 44-45 -0.2853911514698808 1104 46-47 -0.5480455692896911 1104 48-49 -0.023539302750954505 1104 50-51 -0.18626175277965018 1104 52-53 -0.16281406457346037 1104 54-55 -0.25253594556643577 1104 56-57 -0.29942471817843597 1104 58-59 -0.5205473901020596 1104 60-61 -0.5900289157995289 1104 62-63 -0.583432773993593 1104 64-65 -0.6511158793411553 1104 66-67 -0.6428823539504833 1104 68-69 -0.7466279920599739 1104 70-71 -0.5682520409341265 1104 72-73 -0.32684408295627065 1104 74-75 -0.2958384388425195 1104 76-77 -0.30288067163672494 1104 78-79 -0.383695362963838 1104 80-81 -0.3724843696123763 1104 82-83 0.10046567660383943 1104 84-85 -0.11614599130867731 1104 86-87 -0.23443267924834643 1104 88-89 -0.26341676845371964 1104 90-91 -0.41174611842428277 1104 92-93 -0.40004432012374735 1104 94-95 -0.09851122713056526 1104 96-97 -0.45305107546573 1104 98-99 -0.06359904735028366 1104 100-101 0.11963609065824343 1105 1 -0.019804728489852153 1105 2 0.018000212816609462 1105 3 0.05670755858302101 1105 4 0.14783007242927226 1105 5 0.03009223649634407 1105 6 -0.006396062698620142 1105 7 -0.11299140407632535 1105 8 0.05331671217637535 1105 9 0.06185245824171659 1105 10-11 -0.04679314575403026 1105 12-13 0.03714377069615438 1105 14-15 -0.23168762796012743 1105 16-17 -0.08249646976328506 1105 18-19 0.05248262956463634 1105 20-21 0.0219337061183964 1105 22-23 0.10347248151351351 1105 24-25 0.005717125172978399 1105 26-27 0.06363469745631534 1105 28-29 -0.08414160109735036 1105 30-31 -0.019118473507980127 1105 32-33 -0.18454925526608434 1105 34-35 -0.12870071135071726 1105 36-37 0.06112370766326336 1105 38-39 0.1613233758662389 1105 40-41 0.07217587417641624 1105 42-43 -0.11540223348176681 1105 44-45 -0.09500608342695216 1105 46-47 -0.11460645920922019 1105 48-49 0.027218279611062712 1105 50-51 -0.3374578436895348 1105 52-53 -0.3100827578528609 1105 54-55 -0.07420550365792877 1105 56-57 -0.2847351177181352 1105 58-59 -0.2354514500112188 1105 60-61 -0.07905312530722597 1105 62-63 0.05223026313579027 1105 64-65 0.07170400087539974 1105 66-67 0.24966343662899249 1105 68-69 0.10908918300344439 1105 70-71 0.24398478855317407 1105 72-73 0.2753685108697397 1105 74-75 0.5258180424533023 1105 76-77 0.6780184888329934 1105 78-79 0.526828842184937 1105 80-81 0.385337733659437 1105 82-83 0.25630185050778564 1105 84-85 0.3973013135637373 1105 86-87 0.2424954942720099 1105 88-89 0.5909578555884067 1105 90-91 0.8143124481845554 1105 92-93 0.9240640603586989 1105 94-95 1.0248966729975884 1105 96-97 0.7641893719350392 1105 98-99 0.7345933035960392 1105 100-101 0.6694172722774994 1106 1 -0.16516878220761555 1106 2 0.020413650839515185 1106 3 -0.14614458884049952 1106 4 -0.1508087485258045 1106 5 -0.14049492615865233 1106 6 -0.11264096766279863 1106 7 -0.15998908717582339 1106 8 -0.038045324979847805 1106 9 -0.00432401234651536 1106 10-11 -0.13005407594666707 1106 12-13 -0.08634112654501536 1106 14-15 0.12918839641023538 1106 16-17 0.19510253482760476 1106 18-19 -0.07920795695443417 1106 20-21 -0.018199086604816728 1106 22-23 -0.10709462278992277 1106 24-25 -0.013426801544312639 1106 26-27 -0.07946608771551666 1106 28-29 -0.19544936271402946 1106 30-31 -0.1686845909834247 1106 32-33 -0.3140194788040702 1106 34-35 -0.24698062725580883 1106 36-37 -0.4621820588446539 1106 38-39 -0.4117957514345747 1106 40-41 -0.4339676663907923 1106 42-43 -0.48477355969277625 1106 44-45 -0.4789063065358903 1106 46-47 -0.7458572636885634 1106 48-49 -0.7696480051960819 1106 50-51 -0.7099317785588894 1106 52-53 -0.7536565768771837 1106 54-55 -0.720116603327547 1106 56-57 -0.7680502161864027 1106 58-59 -0.620920407835051 1106 60-61 -0.47902684564876097 1106 62-63 -0.3220322974683256 1106 64-65 -0.32998862365801074 1106 66-67 -0.831363722593558 1106 68-69 -0.651698884958968 1106 70-71 -0.6922950844463536 1106 72-73 -0.7056377318899472 1106 74-75 -0.43866419464285045 1106 76-77 -0.5138846947599163 1106 78-79 -0.4224862777361196 1106 80-81 -0.8177718471531961 1106 82-83 -0.6887548921017306 1106 84-85 -0.5525474443201617 1106 86-87 -0.31573003882963135 1106 88-89 -0.017435009216161035 1106 90-91 -0.019506254351163932 1106 92-93 -0.28590772923241303 1106 94-95 -0.05489158803986527 1106 96-97 -0.08098053410177286 1106 98-99 -0.05089496173197361 1106 100-101 0.3941458945798111 1107 1 -0.13085505671742226 1107 2 -0.17687813716280942 1107 3 -0.28560779986748486 1107 4 -0.024061514978924947 1107 5 -0.0881021868847256 1107 6 -0.05915620742206329 1107 7 0.0063621067670993625 1107 8 0.0851103729428857 1107 9 -0.0841871457186798 1107 10-11 -0.08060979328310935 1107 12-13 -0.12411291843009309 1107 14-15 0.04882778681986366 1107 16-17 -0.07897046070723235 1107 18-19 0.09094104323970242 1107 20-21 0.06331421178384034 1107 22-23 0.115883925064864 1107 24-25 0.14653000295966478 1107 26-27 -0.19136183611388446 1107 28-29 -0.0030352683634191635 1107 30-31 0.017771922314970823 1107 32-33 0.0342745417744581 1107 34-35 0.006999182489863642 1107 36-37 0.06610128419553263 1107 38-39 -0.03690495353125556 1107 40-41 0.027022917961758708 1107 42-43 -0.1760349652451012 1107 44-45 -0.2802434255419044 1107 46-47 -0.539574503059562 1107 48-49 -0.027789628181906778 1107 50-51 0.21195232161583277 1107 52-53 0.1923247859649777 1107 54-55 0.20652385483591473 1107 56-57 0.1329398828235 1107 58-59 -0.03411687454054402 1107 60-61 -0.27555907240083855 1107 62-63 0.14133646587859516 1107 64-65 0.3325040962022143 1107 66-67 0.33833633564756127 1107 68-69 0.5425716450352063 1107 70-71 0.7371846282307999 1107 72-73 0.5010818371257777 1107 74-75 0.5196570051241842 1107 76-77 0.26711656713097653 1107 78-79 -0.052934249003833145 1107 80-81 0.030971115378353176 1107 82-83 -0.13576760807920962 1107 84-85 -0.11835990791946216 1107 86-87 -0.313024253545219 1107 88-89 -0.3616278843860279 1107 90-91 -0.11112681346589781 1107 92-93 0.3509467650405469 1107 94-95 0.29579274509932674 1107 96-97 0.0347008869815113 1107 98-99 0.0906552226982349 1107 100-101 0.953794506499829 1108 1 0.054144943282580016 1108 2 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-0.8325719541240844 2316 86-87 -1.509153241444011 2316 88-89 -1.1081212837259606 2316 90-91 -1.0088853393184856 2316 92-93 -1.3165449841343744 2316 94-95 -1.6753981239875806 2316 96-97 -1.6779985079579802 2316 98-99 -1.9377413669607328 2316 100-101 -1.946336131563978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 12.0 6 20.0 7 50.0 8 108.0 9 228.0 10 428.0 11 738.0 12 1385.0 13 2052.0 14 2601.0 15 3897.0 16 5978.0 17 9710.0 18 8590.0 19 4081.0 20 2965.0 21 2992.0 22 2980.0 23 3217.0 24 3405.0 25 3872.0 26 4425.0 27 5098.0 28 6155.0 29 7478.0 30 9020.0 31 11150.0 32 14253.0 33 19244.0 34 27329.0 35 42334.0 36 61059.0 37 94440.0 38 83359.0 39 8814.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.96413990952223 20.159329140461217 16.5885468387951 18.28798411122145 2 15.584610329611687 23.242398114110433 40.0464856783094 21.12650587796848 3 18.40925479576513 29.18475851925032 32.12980706679339 20.276179618191158 4 11.75108551115502 19.32044465712402 38.87000990136127 30.058459930359692 5 11.519844043919985 39.985798117147894 33.67941234196546 14.814945496966663 6 27.11760126843274 39.78384600626719 17.386922705431193 15.711630019868878 7 24.93445034213724 37.597828316129075 21.182738553342755 16.28498278839093 8 26.38834065093181 33.955935634536125 19.806691908889835 19.84903180564223 9 28.05393044348837 16.81599566016058 20.824172552720892 34.30590134363016 10-11 23.779748253475407 29.0064215651683 26.30041105073742 20.91341913061887 12-13 26.889941812319645 27.2134639918932 27.530590709157533 18.366003486629626 14-15 23.826005076377204 27.07669475360165 28.81516650384918 20.282133666171966 16-17 18.917664337237277 30.842409581165803 29.46956048099887 20.77036560059805 18-19 18.775649266880276 27.351904082492233 35.439485923086934 18.43296072754056 20-21 20.41532792470555 26.19792137569381 35.67070145300823 17.716049246592412 22-23 20.07982887511853 26.209341301519395 35.26705184466448 18.443777978697597 24-25 19.633382583191832 26.555891238670693 35.81548944803405 17.995236730103425 26-27 19.470263809593117 26.484163265126103 35.88785170385758 18.157721221423202 28-29 18.958583952921927 26.132543259100316 36.79402460418185 18.114848183795907 30-31 18.919821863115985 27.140955936591382 36.184633433413126 17.754588766879507 32-33 18.74113784062116 27.549159486893377 35.449960637127234 18.259742035358222 34-35 19.085700802473355 26.7042359743579 35.81811927060707 18.391943952561675 36-37 19.566655567145123 26.80501374944043 35.232417365532235 18.39591331788221 38-39 19.41037088328594 26.95174889711922 35.627266542660344 18.010613676934494 40-41 18.8690604171146 27.236381478479956 35.76622617689055 18.1283319275149 42-43 18.899966586791088 27.78997537567805 34.78149636038627 18.52856167714459 44-45 19.208572965916307 27.906353644767485 33.88456178110225 19.00051160821396 46-47 19.3327937936768 28.32748587016206 32.88078893340949 19.458931402751652 48-49 19.738661397701737 28.609862108659172 32.38516956908129 19.266306924557803 50-51 20.45005987126025 28.881873893263766 31.473759187426815 19.19430704804917 52-53 19.84969336652899 29.205046386444177 31.58633479832316 19.35892544870367 54-55 19.40424236944647 29.215741620780065 31.67564551803525 19.704370491738207 56-57 20.037907438811132 29.78755074723302 30.601932198829918 19.572609615125927 58-59 19.8978770511144 28.832918387643808 31.31046390854582 19.958740652695973 60-61 19.979690080776585 28.98353374952864 31.553808054724314 19.48296811497046 62-63 20.662310655761203 28.91638531952288 31.52624301777614 18.895061006939773 64-65 20.35733108696659 28.5868177377705 31.964306584956546 19.09154459030637 66-67 19.932608997669103 29.234155065461447 31.534512528860592 19.298723408008854 68-69 19.947053077029945 29.576512824357792 31.2322894637608 19.244144634851466 70-71 19.8963334090453 29.586105457215755 30.949031144081346 19.5685299896576 72-73 19.87582502155586 29.28752097699312 30.567420772570802 20.26923322888022 74-75 19.929190933087526 29.777296553488302 30.37876565969749 19.914746853726683 76-77 19.97616175604722 28.97669762036549 31.386212630079406 19.660927993507883 78-79 19.554857731331303 28.674695075561278 31.490849510334684 20.279597682772735 80-81 19.418067708700868 30.250048514572015 30.529117564039236 19.802766212687885 82-83 19.45921629032916 29.80462939787837 30.178412535297145 20.557741776495327 84-85 19.296582034372612 29.0220002800617 30.21446772288887 21.466949962676818 86-87 19.673607910504042 29.363379958674496 30.11844145075892 20.844570680062542 88-89 20.011224483045297 28.382615944058408 30.531586224538177 21.074573348358115 90-91 19.423141175705194 28.457442409499855 30.979614655570707 21.139801759224238 92-93 19.436778123880146 28.811974529316096 30.626016484273784 21.12523086252998 94-95 19.121645610653776 28.55859113993556 30.61758913981648 21.70217410959418 96-97 19.533357002511725 28.49265557155553 29.891856847044917 22.082130578887828 98-99 19.70717045374356 28.61720238515278 29.777692999788304 21.897934161315362 100-101 19.41392991595119 28.89296402728554 30.121055870073903 21.572050186689367 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1815.0 1 1226.0 2 710.0 3 829.5 4 711.0 5 644.0 6 1139.5 7 2498.0 8 3941.5 9 4395.5 10 3567.5 11 2568.0 12 2370.5 13 2366.5 14 2384.5 15 2293.5 16 2157.5 17 2110.5 18 2113.0 19 2105.5 20 2165.0 21 2344.5 22 2518.5 23 2700.0 24 2866.5 25 3136.0 26 3539.5 27 3970.0 28 4260.5 29 5032.0 30 6172.0 31 6975.5 32 8351.0 33 9976.0 34 11232.5 35 12387.0 36 14338.5 37 15767.5 38 15907.5 39 15514.5 40 14912.0 41 14512.0 42 13675.5 43 13856.0 44 14754.0 45 14982.0 46 14426.0 47 14468.5 48 19936.0 49 19470.5 50 13708.5 51 11798.5 52 9930.0 53 9229.5 54 8748.0 55 8102.5 56 7418.5 57 6757.0 58 6021.0 59 5502.5 60 4935.5 61 4082.0 62 3218.5 63 2428.5 64 1711.5 65 1274.0 66 935.0 67 702.0 68 499.0 69 289.5 70 203.0 71 175.0 72 155.5 73 128.0 74 99.0 75 78.0 76 56.0 77 30.5 78 21.0 79 19.0 80 12.0 81 6.0 82 3.5 83 2.0 84 1.0 85 2.0 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07122805547408556 2 0.0 3 0.0 4 0.0 5 0.0026462435470248503 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.205202955854042E-4 12-13 0.005843787833013211 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.615608867562126E-4 24-25 6.615608867562126E-4 26-27 4.410405911708084E-4 28-29 0.0029770239904029568 30-31 3.307804433781063E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.307804433781063E-4 40-41 1.102601477927021E-4 42-43 0.01312095758733155 44-45 2.205202955854042E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.205202955854042E-4 82-83 3.307804433781063E-4 84-85 3.307804433781063E-4 86-87 0.0 88-89 0.0 90-91 0.007607950197696445 92-93 0.002315463103646744 94-95 0.0 96-97 0.0 98-99 2.205202955854042E-4 100-101 0.010143933596928594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 453473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.326624530837414 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61147048900753 34.272127330797375 2 12.632276722351618 11.451569279273386 3 4.454800452032682 6.0576320234727055 4 2.147570453024268 3.8936847831100554 5 1.1912285507783704 2.6997184625772395 6 0.7591201693520735 2.0645012934004288 7 0.534487366768063 1.695855573298038 8 0.36232570233984146 1.3138400854263963 9 0.297643362358166 1.214205203673412 >10 1.763274710116701 15.41755056118629 >50 0.1586451296536098 4.910195251233216 >100 0.07687433751730542 6.121542461556395 >500 0.005386100080830316 1.5296088262289418 >1k 0.0034275182332556557 2.6315397348515037 >5k 0.0014689363856809953 4.726429129914618 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8838 1.9489583723838022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7316 1.6133264825028173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5142 1.1339153599001486 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2768 0.6104001781803988 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1981 0.43685070555468575 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1945 0.42891197491361116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1408 0.31049257618424914 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1318 0.29064574958156275 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1273 0.2807223362802196 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1164 0.2566856240614105 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.16539022168905315 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 749 0.16516970139346776 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 714 0.1574514910479786 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 669 0.1475280777466354 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 0.14488183419961057 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 657 0.14488183419961057 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 628 0.13848674562763386 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 538 0.11863991902494747 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 512 0.11290639133972695 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 510 0.11246535074855614 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 508 0.11202431015738534 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 491 0.10827546513243345 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.205202955854042E-4 0.0 10-11 0.0 0.0 0.0 2.205202955854042E-4 0.0 12-13 0.0 0.0 0.0 2.205202955854042E-4 0.0 14-15 0.0 0.0 0.0 2.205202955854042E-4 0.0 16-17 0.0 0.0 0.0 3.307804433781063E-4 0.0 18-19 0.0 0.0 0.0 5.513007389635105E-4 0.0 20-21 0.0 0.0 0.0 8.820811823416168E-4 0.0 22-23 0.0 0.0 0.0 0.0019846826602686377 0.0 24-25 0.0 0.0 0.0 0.0029770239904029568 0.0 26-27 0.0 0.0 0.0 0.0036385848771591693 0.0 28-29 0.0 0.0 0.0 0.005733527685220509 0.0 30-31 0.0 0.0 0.0 0.013341477882916954 0.0 32-33 0.0 0.0 0.0 0.021721249115162315 0.0 34-35 0.0 0.0 0.0 0.03517298714587197 0.0 36-37 0.0 0.0 0.0 0.050940188280228366 0.0 38-39 0.0 0.0 0.0 0.07266143739539069 0.0 40-41 0.0 0.0 0.0 0.09680840976199245 0.0 42-43 0.0 0.0 0.0 0.11533211459116641 0.0 44-45 0.0 0.0 0.0 0.12823255188291255 0.0 46-47 0.0 0.0 0.0 0.15105640247600188 0.0 48-49 0.0 0.0 0.0 0.1727776515911642 0.0 50-51 0.0 0.0 0.0 0.19163213686371625 0.0 52-53 0.0 0.0 0.0 0.23154631036467443 0.0 54-55 0.0 0.0 0.0 0.29891526066601537 0.0 56-57 0.0 0.0 0.0 0.40079563722647216 0.0 58-59 0.0 0.0 0.0 0.5379592610805936 0.0 60-61 0.0 0.0 0.0 0.7042315639519883 0.0 62-63 0.0 0.0 0.0 0.8094197449462261 0.0 64-65 0.0 0.0 0.0 0.9328008503262598 0.0 66-67 0.0 0.0 0.0 1.1392078469941982 0.0 68-69 0.0 0.0 0.0 1.4162915983972586 0.0 70-71 0.0 0.0 0.0 1.7187351837926403 0.0 72-73 0.0 0.0 0.0 2.0618647637235292 0.0 74-75 0.0 0.0 0.0 2.413925415625627 0.0 76-77 0.0 0.0 0.0 2.7451468995949044 0.0 78-79 0.0 0.0 0.0 3.052882972084336 0.0 80-81 0.0 0.0 0.0 3.2837677215622536 0.0 82-83 0.0 0.0 0.0 3.618517530260897 0.0 84-85 0.0 0.0 0.0 4.204439955631317 0.0 86-87 0.0 0.0 0.0 4.928408086038198 0.0 88-89 0.0 0.0 0.0 5.673325644525693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTA 160 0.0 41.560234 5 CTGGTCG 305 0.0 35.821667 9 GTTAGCC 35 2.0536501E-4 33.92672 64-65 GGGCGCG 85 1.6294112E-5 33.53128 7 GGTATAT 95 3.4665794E-5 30.024849 1 GGGTATG 80 4.4879326E-4 29.689154 7 GTATGGT 130 3.0081355E-7 29.25498 1 TAAGGTG 100 4.9484268E-5 28.498446 5 CCCTGGT 405 0.0 28.149717 7 CATGGGT 320 0.0 26.717293 4 TGGGGCG 125 7.3811243E-6 26.598547 5 GCTACGG 45 8.9489366E-4 26.387447 72-73 ATAACCC 415 0.0 26.323864 3 GAATAAC 435 0.0 25.135746 1 GTTCTAG 95 0.0012195077 25.020706 1 GTAGGAT 95 0.0012257992 24.998636 3 CTAGATC 230 1.3460522E-10 24.781256 4 ACCCTGG 520 0.0 24.662117 6 TAGATCA 235 1.7644197E-10 24.253996 5 TACCTGG 275 1.8189894E-12 24.180498 2 >>END_MODULE