##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937327_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51545 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.904627024929674 33.0 31.0 34.0 31.0 34.0 2 32.024696866815404 33.0 31.0 34.0 30.0 34.0 3 31.97701037928024 34.0 31.0 34.0 30.0 34.0 4 35.63870404500921 37.0 35.0 37.0 35.0 37.0 5 35.636065573770495 37.0 35.0 37.0 35.0 37.0 6 35.72061305655253 37.0 35.0 37.0 35.0 37.0 7 35.68346105344844 37.0 35.0 37.0 35.0 37.0 8 35.68462508487729 37.0 35.0 37.0 35.0 37.0 9 37.27876612668542 39.0 37.0 39.0 35.0 39.0 10-11 37.20453002231061 39.0 37.0 39.0 35.0 39.0 12-13 37.125181879910755 39.0 37.0 39.0 34.0 39.0 14-15 38.207110291977884 40.0 38.0 41.0 34.0 41.0 16-17 38.12979920457852 40.0 38.0 41.0 33.5 41.0 18-19 38.1205742555049 40.0 38.0 41.0 34.0 41.0 20-21 38.077136482685034 40.0 38.0 41.0 34.0 41.0 22-23 38.10076632069066 40.0 38.0 41.0 34.0 41.0 24-25 38.050043651178584 40.0 38.0 41.0 34.0 41.0 26-27 37.92275681443399 40.0 37.5 41.0 34.0 41.0 28-29 37.74464060529634 40.0 37.0 41.0 34.0 41.0 30-31 37.393597827141335 39.5 36.5 41.0 33.0 41.0 32-33 36.96772722863517 39.0 35.0 41.0 33.0 41.0 34-35 36.819439324861776 39.0 35.0 41.0 33.0 41.0 36-37 36.59953438742846 39.0 35.0 41.0 32.0 41.0 38-39 36.36249878746726 39.0 35.0 41.0 31.0 41.0 40-41 35.99793384421379 39.0 35.0 41.0 30.5 41.0 42-43 35.54139101755747 38.0 35.0 40.0 30.0 41.0 44-45 35.068221941992434 38.0 35.0 40.0 26.5 41.0 46-47 34.36454554272965 38.0 35.0 40.0 21.5 41.0 48-49 33.73098263653118 38.0 34.5 40.0 7.0 41.0 50-51 32.654641575322536 37.5 33.0 39.5 2.0 40.5 52-53 32.65786206227568 37.5 33.0 39.5 2.0 40.5 54-55 32.82461926472014 38.0 33.0 40.0 2.0 41.0 56-57 32.46060723639538 38.0 33.0 40.0 2.0 41.0 58-59 32.12814045979242 38.0 33.0 40.0 2.0 41.0 60-61 31.71228053157435 37.5 32.5 40.0 2.0 41.0 62-63 31.248666213987775 37.0 31.0 40.0 2.0 41.0 64-65 30.80874963624018 36.0 31.0 39.5 2.0 41.0 66-67 30.329061984673586 36.0 30.5 39.0 2.0 41.0 68-69 29.776389562518187 35.0 29.0 39.0 2.0 40.0 70-71 29.221224173052672 35.0 28.0 38.5 2.0 40.0 72-73 28.695081967213113 35.0 27.0 38.0 2.0 40.0 74-75 28.12689882626831 35.0 26.0 37.0 2.0 39.0 76-77 27.605490348239403 34.0 26.0 36.5 2.0 39.0 78-79 27.079260840042682 34.0 24.5 36.0 2.0 39.0 80-81 26.504985934620237 34.0 21.5 35.5 2.0 37.5 82-83 25.957784460180427 33.5 19.0 35.0 2.0 37.0 84-85 25.424105150839072 33.0 14.5 35.0 2.0 36.5 86-87 24.85880298768067 32.5 7.0 35.0 2.0 36.0 88-89 24.338442137937726 32.0 2.0 35.0 2.0 36.0 90-91 23.759103695799787 31.5 2.0 35.0 2.0 35.0 92-93 23.17323697739839 31.0 2.0 34.0 2.0 35.0 94-95 22.48688524590164 31.0 2.0 34.0 2.0 35.0 96-97 21.738597342128237 30.5 2.0 34.0 2.0 35.0 98-99 20.75958870889514 29.5 2.0 34.0 2.0 35.0 100-101 18.102279561548162 24.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9565913111098006 1101 2 0.3588809076505761 1101 3 0.6302308207698673 1101 4 0.6720514850917141 1101 5 0.39489803633208 1101 6 0.8784411276481663 1101 7 0.6999705053292544 1101 8 0.3165532069730119 1101 9 0.9838451478885943 1101 10-11 0.647953422385207 1101 12-13 0.9362168881854842 1101 14-15 0.6781947417735097 1101 16-17 1.2848068248458375 1101 18-19 1.403822402047858 1101 20-21 0.8139841267285561 1101 22-23 0.6026890598148569 1101 24-25 0.7125724289999766 1101 26-27 0.3078659550709517 1101 28-29 0.9051663087100863 1101 30-31 0.8501298057533688 1101 32-33 1.0544687478343064 1101 34-35 1.199997007297263 1101 36-37 1.1864801340883844 1101 38-39 1.4899825280821872 1101 40-41 1.0213814690608558 1101 42-43 1.2461625813661428 1101 44-45 1.9276204546405111 1101 46-47 2.846496411834238 1101 48-49 2.595994759799872 1101 50-51 2.4617909004605565 1101 52-53 2.2132887980461184 1101 54-55 2.300086821548369 1101 56-57 2.3125197146976006 1101 58-59 2.678173269706381 1101 60-61 2.6021537389326816 1101 62-63 3.0439401393749783 1101 64-65 2.9615342291435347 1101 66-67 4.305928481062278 1101 68-69 4.177533751253346 1101 70-71 4.274433804145843 1101 72-73 4.584214232505289 1101 74-75 4.138302271507353 1101 76-77 3.572304724668797 1101 78-79 4.316965275200143 1101 80-81 4.0370153978179495 1101 82-83 3.134927495775745 1101 84-85 3.1440975084050535 1101 86-87 3.6019342115833233 1101 88-89 3.3427875355842502 1101 90-91 3.7352916274037646 1101 92-93 3.3546309901507847 1101 94-95 3.767919008294836 1101 96-97 3.4334383203721117 1101 98-99 3.263853024561115 1101 100-101 4.591553275123019 1102 1 0.6597163111098006 1102 2 0.8432559076505761 1102 3 0.5052308207698673 1102 4 0.4376764850917141 1102 5 0.48864803633208 1102 6 0.3940661276481663 1102 7 -0.19065449467074558 1102 8 0.3634282069730119 1102 9 0.8900951478885943 1102 10-11 0.41357842238520703 1102 12-13 0.3580918881854842 1102 14-15 1.2641322417735097 1102 16-17 1.3394943248458375 1102 18-19 1.411634902047858 1102 20-21 1.3061716267285561 1102 22-23 1.2120640598148569 1102 24-25 1.2750724289999766 1102 26-27 1.5422409550709517 1102 28-29 1.4911038087100863 1102 30-31 1.9048173057533688 1102 32-33 1.2966562478343064 1102 34-35 1.887497007297263 1102 36-37 2.5849176340883844 1102 38-39 1.5524825280821872 1102 40-41 1.8104439690608558 1102 42-43 1.9258500813661428 1102 44-45 1.6151204546405111 1102 46-47 1.471496411834238 1102 48-49 1.580369759799872 1102 50-51 1.0946034004605565 1102 52-53 1.2523512980461184 1102 54-55 1.6125868215483692 1102 56-57 2.0156447146976006 1102 58-59 2.467235769706381 1102 60-61 2.7505912389326816 1102 62-63 3.0908151393749783 1102 64-65 3.1724717291435347 1102 66-67 3.3996784810622778 1102 68-69 3.263471251253346 1102 70-71 3.5088088041458434 1102 72-73 4.357651732505289 1102 74-75 4.435177271507353 1102 76-77 4.095742224668797 1102 78-79 4.160715275200143 1102 80-81 3.9979528978179495 1102 82-83 3.869302495775745 1102 84-85 4.0972225084050535 1102 86-87 4.187871711583323 1102 88-89 3.3349750355842502 1102 90-91 3.2040416274037646 1102 92-93 3.7999434901507847 1102 94-95 3.744481508294836 1102 96-97 3.9412508203721117 1102 98-99 4.404478024561115 1102 100-101 2.185303275123019 1103 1 0.4824533800753166 1103 2 0.7624369421333377 1103 3 0.4201015104250416 1103 4 0.6348747609537853 1103 5 0.4353075190906992 1103 6 0.5853376793723015 1103 7 0.7166730915361512 1103 8 0.5121351035247343 1103 9 0.6519485961644591 1103 10-11 0.5695590258334846 1103 12-13 0.7487168881854842 1103 14-15 0.9836904314286841 1103 16-17 0.7721451869148055 1103 18-19 0.6955788675650965 1103 20-21 0.517647919832001 1103 22-23 0.4240791460217537 1103 24-25 0.643068118655151 1103 26-27 0.782812075760603 1103 28-29 0.8916964811238799 1103 30-31 0.7760457540292265 1103 32-33 0.9151907305929257 1103 34-35 0.6994582141938182 1103 36-37 -0.15619227970471883 1103 38-39 0.06487476946149684 1103 40-41 -0.2776487033529378 1103 42-43 -0.6953783669097149 1103 44-45 -0.40481489018707606 1103 46-47 -0.13922557092438126 1103 48-49 -0.40508282640702475 1103 50-51 -2.2154720305739275 1103 52-53 -2.4984568916090524 1103 54-55 -2.1907536956930116 1103 56-57 -1.395185026681709 1103 58-59 -0.9042620751212063 1103 60-61 0.04692744582923325 1103 62-63 -0.2723314123491605 1103 64-65 -0.8242955122357749 1103 66-67 -1.9847503982480674 1103 68-69 -1.0153541797811378 1103 70-71 -1.3168916268886406 1103 72-73 -0.806410767494711 1103 74-75 -1.0580878146995438 1103 76-77 -0.6017254477449967 1103 78-79 -0.2762459316964083 1103 80-81 -0.723491067699289 1103 82-83 -0.0032729352587388405 1103 84-85 -0.4760533536639109 1103 86-87 -1.1154688056580575 1103 88-89 -2.0543029816571305 1103 90-91 -2.2374993208721 1103 92-93 -1.6119638374354217 1103 94-95 -1.6270163365327512 1103 96-97 -1.1371435761796107 1103 98-99 -0.616265509921643 1103 100-101 0.5182774130540544 1104 1 0.6325172370357244 1104 2 0.6245059076505761 1104 3 0.4392585985476458 1104 4 0.6610561147213403 1104 5 0.8561248881839347 1104 6 0.10066334987038772 1104 7 0.3718455053292544 1104 8 0.5740763551211572 1104 9 0.8032895923330372 1104 10-11 0.1465066631259475 1104 12-13 0.34130948077807943 1104 14-15 0.25284751955128826 1104 16-17 -0.05576030478379579 1104 18-19 0.35202842056637706 1104 20-21 0.23412301561744187 1104 22-23 1.153325633888933 1104 24-25 0.34741039196293855 1104 26-27 0.7259793809968755 1104 28-29 0.8779672346360101 1104 30-31 0.9088682316792926 1104 32-33 1.3823043959824588 1104 34-35 1.4274275628528201 1104 36-37 1.3600912451994986 1104 38-39 2.2104686391933015 1104 40-41 1.967562024616413 1104 42-43 2.4866139702550285 1104 44-45 2.5763473064923588 1104 46-47 1.7825496525749784 1104 48-49 2.4860410560961697 1104 50-51 3.19385108564574 1104 52-53 3.649052686935004 1104 54-55 3.0448784882150406 1104 56-57 2.042554436919822 1104 58-59 1.8269001215582286 1104 60-61 2.3026745722660102 1104 62-63 2.7586392134490545 1104 64-65 2.23381432173613 1104 66-67 1.402861351432648 1104 68-69 1.4124874549570485 1104 70-71 2.036875933775473 1104 72-73 2.443589232505289 1104 74-75 2.860813845581429 1104 76-77 3.6157075024465755 1104 78-79 3.245784719644586 1104 80-81 3.0089482681883233 1104 82-83 2.683827958738707 1104 84-85 2.944444730627275 1104 86-87 3.064607822694434 1104 88-89 3.060958831880548 1104 90-91 3.1852337570333944 1104 92-93 3.1613439531137466 1104 94-95 2.6134051194059467 1104 96-97 3.2083225796313712 1104 98-99 3.4753692282648174 1104 100-101 1.9295162380859807 1105 1 0.7338640383825243 1105 2 0.7972331803778481 1105 3 0.9796626389516874 1105 4 0.8586992123644421 1105 5 0.5770003090593505 1105 6 0.9602593094663447 1105 7 0.6627545962383437 1105 8 0.6986554797002853 1105 9 1.1315724206158677 1105 10-11 1.0148568314761164 1105 12-13 0.8532623427309431 1105 14-15 1.1750697417735125 1105 16-17 1.0738693248458375 1105 18-19 0.75623717477513 1105 20-21 0.9588704903649159 1105 22-23 1.0883424689057648 1105 24-25 1.1253565199090687 1105 26-27 1.590962545980041 1105 28-29 1.6338594905282662 1105 30-31 0.9965786693897343 1105 32-33 1.2796107932888532 1105 34-35 1.7718720072972616 1105 36-37 2.158071043179291 1105 38-39 1.9902666189912779 1105 40-41 2.2756428326972156 1105 42-43 2.2295432631843255 1105 44-45 2.7199500000950536 1105 46-47 3.3463543663796926 1105 48-49 2.58519930525442 1105 50-51 2.59957499136965 1105 52-53 3.0465274344097573 1105 54-55 3.260030003366552 1105 56-57 3.2203322146975992 1105 58-59 3.7795937242518356 1105 60-61 4.873886693478138 1105 62-63 5.111837866647708 1105 64-65 5.323039910961722 1105 66-67 4.532490981062278 1105 68-69 4.37242011488971 1105 70-71 4.439064485964025 1105 72-73 4.248134687050744 1105 74-75 4.032194316961899 1105 76-77 4.256111542850615 1105 78-79 4.337703911563782 1105 80-81 3.315850625090679 1105 82-83 3.607228632139382 1105 84-85 4.081313417495963 1105 86-87 4.435314893401504 1105 88-89 5.2146625355842495 1105 90-91 4.982876854676494 1105 92-93 5.152926444696238 1105 94-95 5.743203099203928 1105 96-97 5.249063320372109 1105 98-99 5.444392797288387 1105 100-101 5.67564418421393 1106 1 0.07022767474616387 1106 2 0.010869544014212806 1106 3 0.0023899116789571906 1106 4 0.1905173941826206 1106 5 0.25427303633208 1106 6 -0.8033770541700136 1106 7 -0.2872454037616521 1106 8 -0.02861724757244133 1106 9 -0.11842757938413229 1106 10-11 -0.912415895796606 1106 12-13 0.06689870636730433 1106 14-15 -0.5022029854992169 1106 16-17 0.23296023393674403 1106 18-19 -0.31421737067941535 1106 20-21 0.0043250358194626415 1106 22-23 0.6701606507239504 1106 24-25 -0.6496434800909299 1106 26-27 -1.1544919994745015 1106 28-29 -0.27523141856264033 1106 30-31 0.5056695784806351 1106 32-33 0.8250653387433999 1106 34-35 -0.17358253815728375 1106 36-37 -0.09192895682070912 1106 38-39 -0.9915515628269063 1106 40-41 -0.1789026218482377 1106 42-43 -0.4022749186338572 1106 44-45 0.8381318182768709 1106 46-47 1.4963543663796912 1106 48-49 1.314744759799872 1106 50-51 1.7700295368241967 1106 52-53 1.3738001616824853 1106 54-55 2.2191209124574627 1106 56-57 2.3657867601521474 1106 58-59 0.3727755424336543 1106 60-61 -0.007931488340044979 1106 62-63 1.445928775738615 1106 64-65 0.9366762745980814 1106 66-67 1.2484000719713677 1106 68-69 1.1860564785260728 1106 70-71 -0.13820824130870335 1106 72-73 -0.9200471311310743 1106 74-75 0.11628522605280622 1106 76-77 0.1220206337597034 1106 78-79 0.34679482065469003 1106 80-81 0.19539607963612937 1106 82-83 1.2435922685030185 1106 84-85 0.7676770538596003 1106 86-87 0.5784967115833233 1106 88-89 1.7532988992206135 1106 90-91 2.135149581949218 1106 92-93 1.4597446265144178 1106 94-95 1.2182030992039259 1106 96-97 -0.19866395235516165 1106 98-99 -1.012425384529795 1106 100-101 -1.0675376339678877 1107 1 0.4310811070281666 1107 2 0.5530773362220067 1107 3 0.6308685758719079 1107 4 0.24571219937742939 1107 5 0.11651793429126656 1107 6 0.05191051540327152 1107 7 0.4330699951251731 1107 8 0.07898943146280857 1107 9 0.6751716785008384 1107 10-11 0.7322965346301018 1107 12-13 0.5395332147161014 1107 14-15 0.6383350478959571 1107 16-17 0.5636652432131868 1107 18-19 0.49167316735398003 1107 20-21 0.4625810655040681 1107 22-23 0.31394543736588076 1107 24-25 1.073222939204058 1107 26-27 0.789849373438301 1107 28-29 0.7275515127917203 1107 30-31 -0.6037923881241838 1107 32-33 0.8872174723241031 1107 34-35 1.3820760889299137 1107 36-37 1.0244903381700183 1107 38-39 1.1809901811434074 1107 40-41 0.7997615711016692 1107 42-43 1.3987454895294107 1107 44-45 2.790821985252755 1107 46-47 2.281604830201587 1107 48-49 2.164234555718245 1107 50-51 1.804584267807499 1107 52-53 2.1122046143726543 1107 54-55 1.6333138623646946 1107 56-57 2.0856383371465768 1107 58-59 2.500558473788015 1107 60-61 2.974443279749007 1107 62-63 2.102932486313751 1107 64-65 1.4575482597557787 1107 66-67 1.8794297565724811 1107 68-69 0.6985796696206918 1107 70-71 1.1572463041458434 1107 72-73 1.7139973957705976 1107 74-75 2.103066302119597 1107 76-77 1.3583378879341055 1107 78-79 0.5972586425470823 1107 80-81 0.9293942243485631 1107 82-83 1.1341303018981925 1107 84-85 1.2171204675887282 1107 86-87 1.8820681401547539 1107 88-89 1.5554788621148603 1107 90-91 1.26988984168948 1107 92-93 1.269809561579354 1107 94-95 1.3645389062540225 1107 96-97 0.938859238739461 1107 98-99 2.0369716470100947 1107 100-101 2.823696132265873 1108 1 0.36043746495595386 1108 2 0.560403343548014 1108 3 0.7255833848724293 1108 4 0.2750162286814586 1108 5 0.6581191901782333 1108 6 0.4810052302122685 1108 7 0.3205634540472033 1108 8 0.24644102748582952 1108 9 -0.14115485211140566 1108 10-11 0.38012489674418504 1108 12-13 0.5243579138265062 1108 14-15 0.6417364084401811 1108 16-17 0.20207445305096172 1108 18-19 -0.24982343128547768 1108 20-21 0.44138797288240283 1108 22-23 -0.16433818377488763 1108 24-25 0.7370115315640788 1108 26-27 -0.5204593654418659 1108 28-29 -0.201804845136067 1108 30-31 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0.12921141709373885 2311 54-55 -0.20512151178496296 2311 56-57 -1.8463344519690637 2311 58-59 -0.4297136350555242 2311 60-61 -0.661611142019698 2311 62-63 0.029803234613073215 2311 64-65 -0.5730639851421806 2311 66-67 -0.33655663798534263 2311 68-69 -0.48169243922284366 2311 70-71 -0.9737060768065362 2311 72-73 -0.8540298151137584 2311 74-75 -1.141831657064074 2311 76-77 -0.1501655134264439 2311 78-79 0.4561021799620484 2311 80-81 -0.07570781646776581 2311 82-83 -0.4815159566052074 2311 84-85 -1.1706346344520888 2311 86-87 0.0011157592023707252 2311 88-89 0.6456149165366298 2311 90-91 1.174651746451385 2311 92-93 0.9483809901507847 2311 94-95 0.7403892463900732 2311 96-97 0.9490633203721117 2311 98-99 0.9502369531325421 2311 100-101 0.1570294655992086 2312 1 -0.5362658317473432 2312 2 -0.246922663777994 2312 3 -0.8670906078015612 2312 4 -0.8971449434797165 2312 5 -0.3528698208107741 2312 6 -0.5929874437804017 2312 7 0.5147026481864003 2312 8 -0.8312146501698479 2312 9 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32-33 -1.144631630953569 2313 34-35 -1.5826734472481903 2313 36-37 -1.023747138638889 2313 38-39 -3.506703077978422 2313 40-41 -3.9970844400300543 2313 42-43 -3.121971888330826 2313 44-45 -2.55580757566252 2313 46-47 -3.96576684574152 2313 48-49 -4.060255240200128 2313 50-51 -3.3095159177212636 2313 52-53 -3.3989271110447916 2313 54-55 -3.0914851481485996 2313 56-57 -3.9675465731811883 2313 58-59 -2.820406275748166 2313 60-61 -2.977628458037014 2313 62-63 -2.845737890928053 2313 64-65 -3.1315055435837387 2313 66-67 -3.63417568560439 2313 68-69 -4.215458672989076 2313 70-71 -3.3124506655511254 2313 72-73 -3.018531979615922 2313 74-75 -4.1678056830381 2313 76-77 -3.6923733056342343 2313 78-79 -2.592599118739251 2313 80-81 -2.626573617333566 2313 82-83 -2.483680458769708 2313 84-85 -1.9065653703828254 2313 86-87 -1.9252911672045556 2313 88-89 -1.8489738280521166 2313 90-91 -2.5201534483538133 2313 92-93 -2.4076796159098244 2313 94-95 -1.5961908401900118 2313 96-97 -1.6266942553854662 2313 98-99 -0.5616678087722207 2313 100-101 -0.13950733093758672 2314 1 7.089581686230417E-4 2314 2 -0.0813764452906014 2314 3 0.7791278795933962 2314 4 0.35495589685641704 2314 5 0.621920095155609 2314 6 0.4078528923540503 2314 7 0.6365513876821964 2314 8 0.7494576187377149 2314 9 0.4470804420062393 2314 10-11 0.18517768709109106 2314 12-13 0.01342277053842622 2314 14-15 0.3868344476558576 2314 16-17 0.7209281483752434 2314 18-19 0.745274607930206 2314 20-21 0.5952341267285561 2314 22-23 0.12336920687368291 2314 24-25 0.5903297819411506 2314 26-27 0.07946521977683574 2314 28-29 -0.4450175148193267 2314 30-31 -0.1788224001289862 2314 32-33 0.6165091890107774 2314 34-35 0.371872007297263 2314 36-37 0.6019213105589785 2314 38-39 0.9081810574939553 2314 40-41 1.3531829396490878 2314 42-43 1.5830191990132008 2314 44-45 -0.9078574865359528 2314 46-47 -0.634661676401052 2314 48-49 -0.707314063729541 2314 50-51 -0.4913340995394435 2314 52-53 0.3483990921637634 2314 54-55 0.5399765274307171 2314 56-57 0.7909204499917166 2314 58-59 0.9603423873534389 2314 60-61 0.7781647683444461 2314 62-63 0.5080945511396813 2314 64-65 -0.005837094385878316 2314 66-67 -0.34986196011419324 2314 68-69 0.20878375125334614 2314 70-71 -0.3280478135012146 2314 72-73 -1.012293120435885 2314 74-75 -0.683848463786763 2314 76-77 -0.9267761576841451 2314 78-79 -0.37513031303515376 2314 80-81 -0.037892690417344 2314 82-83 -1.1761938277536679 2314 84-85 -1.9983657268890624 2314 86-87 -1.8994444648872637 2314 88-89 -0.7500433467686918 2314 90-91 0.36442765681552913 2314 92-93 0.8160280489743137 2314 94-95 1.237588125941894 2314 96-97 1.0814162615485827 2314 98-99 0.477547877502289 2314 100-101 2.5547885692406638 2315 1 -0.23090868889019944 2315 2 -0.18799409234942388 2315 3 -0.27601917923013275 2315 4 0.3439264850917141 2315 5 -0.43322696366792 2315 6 0.3784411276481663 2315 7 -1.0031544946707456 2315 8 0.019678206973011925 2315 9 -0.29740485211140566 2315 10-11 -4.8407761479296596E-4 2315 12-13 -0.7044081118145158 2315 14-15 0.1938197417735097 2315 16-17 0.5894943248458375 2315 18-19 -0.04149009795214198 2315 20-21 0.7202341267285561 2315 22-23 0.032376559814856876 2315 24-25 -0.06867757100002336 2315 26-27 -0.2155715449290483 2315 28-29 -0.1104586912899137 2315 30-31 -0.6733076942466312 2315 32-33 -0.39084375216569356 2315 34-35 -1.143752992702737 2315 36-37 -2.1572698659116156 2315 38-39 -2.1506424719178128 2315 40-41 -0.8379935309391442 2315 42-43 -1.4960249186338572 2315 44-45 -1.4630045453594889 2315 46-47 -1.130066088165762 2315 48-49 -1.872755240200128 2315 50-51 -1.3038340995394435 2315 52-53 -1.5210862019538816 2315 54-55 -1.8874131784516308 2315 56-57 -1.1640427853023994 2315 58-59 -1.118701730293619 2315 60-61 -1.3900337610673184 2315 62-63 -1.1748098606250217 2315 64-65 -0.5462782708564653 2315 66-67 -2.1862590189377222 2315 68-69 -1.1818412487466539 2315 70-71 -0.6708786958541566 2315 72-73 -1.134535767494711 2315 74-75 -0.760135228492647 2315 76-77 -0.6151952753312031 2315 78-79 -2.6986597247998567 2315 80-81 -1.6739221021820505 2315 82-83 -2.451010004224255 2315 84-85 -1.6840274915949465 2315 86-87 -1.0152532884166767 2315 88-89 -0.46971246441574976 2315 90-91 0.13372912740376464 2315 92-93 -0.9578690098492153 2315 94-95 0.43979400829483595 2315 96-97 0.9646883203721117 2315 98-99 -0.587709475438885 2315 100-101 -0.6428217248769812 2316 1 -0.35590868889019944 2316 2 -0.15674409234942388 2316 3 0.25523082076986725 2316 4 -0.5935735149082859 2316 5 -0.55822696366792 2316 6 0.1284411276481663 2316 7 -0.03440449467074558 2316 8 -0.4178217930269881 2316 9 -1.5786548521114057 2316 10-11 -0.42235907761479297 2316 12-13 0.10809188818548421 2316 14-15 -0.5718052582264903 2316 16-17 -1.1761306751541625 2316 18-19 -1.088365097952142 2316 20-21 -0.045390873271443866 2316 22-23 -1.1394984401851431 2316 24-25 -1.2249275710000234 2316 26-27 -0.8093215449290483 2316 28-29 -0.5323336912899137 2316 30-31 0.18606730575336883 2316 32-33 -0.7033437521656936 2316 34-35 -0.675002992702737 2316 36-37 -1.3760198659116156 2316 38-39 -2.6975174719178128 2316 40-41 -3.447368530939144 2316 42-43 -3.3397749186338572 2316 44-45 -3.681754545359489 2316 46-47 -4.395691088165762 2316 48-49 -4.122755240200128 2316 50-51 -3.0382090995394435 2316 52-53 -3.2554612019538816 2316 54-55 -3.121788178451631 2316 56-57 -2.6171677853023994 2316 58-59 -3.274951730293619 2316 60-61 -3.9837837610673184 2316 62-63 -3.9248098606250217 2316 64-65 -2.6244032708564653 2316 66-67 -1.4987590189377222 2316 68-69 -2.931841248746654 2316 70-71 -2.5771286958541566 2316 72-73 -2.931410767494711 2316 74-75 -3.150760228492647 2316 76-77 -2.521445275331203 2316 78-79 -2.0892847247998567 2316 80-81 -2.2051721021820505 2316 82-83 -1.3260100042242549 2316 84-85 -1.6371524915949465 2316 86-87 -0.7340032884166767 2316 88-89 -1.6728374644157498 2316 90-91 -0.24127087259623536 2316 92-93 -0.1453690098492153 2316 94-95 -0.10708099170516405 2316 96-97 -1.3321866796278883 2316 98-99 -0.43145947543888497 2316 100-101 -0.0959467248769812 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 320.0 3 46.0 4 43.0 5 36.0 6 45.0 7 48.0 8 73.0 9 65.0 10 85.0 11 119.0 12 276.0 13 374.0 14 442.0 15 769.0 16 942.0 17 1157.0 18 1055.0 19 693.0 20 667.0 21 666.0 22 678.0 23 665.0 24 681.0 25 760.0 26 727.0 27 809.0 28 916.0 29 1012.0 30 1182.0 31 1496.0 32 1869.0 33 2418.0 34 3279.0 35 4818.0 36 6309.0 37 8559.0 38 6687.0 39 759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40638070226632 22.27020717829005 16.38362027040171 18.939791849041924 2 14.904126638403797 23.55217611139201 39.807864338220995 21.735832911983195 3 18.45636278780336 28.511978842563785 32.98848786558805 20.043170504044806 4 11.057524016957721 20.014779666290693 39.48699000427832 29.440706312473257 5 10.283536229629341 39.68923806930886 35.98265333903777 14.044572362024036 6 25.762112139357647 39.872851699199 18.142934909401976 16.22210125204137 7 21.624249023000797 37.666478719888005 23.284661598584567 17.424610658526625 8 22.932747457857797 34.55757976396477 21.838119495265683 20.671553282911752 9 24.145909895195317 18.26012561006436 23.673413832662504 33.920550662077815 10-11 21.62143766954797 28.96341166976188 28.203058912753896 21.212091747936253 12-13 24.41862726035388 28.35310130274159 28.842115496791752 18.386155940112776 14-15 21.721674038788706 29.27429154717348 29.728284644922958 19.27574976911486 16-17 17.094698368878433 32.708944922915414 30.599568404067114 19.596788304139043 18-19 16.381094923852935 30.580311585822166 35.39405012974643 17.644543360578467 20-21 17.82614189760588 29.40015746959962 35.11960885329082 17.654091779503677 22-23 17.271711133790156 29.504786897992908 35.18199931962871 18.041502648588228 24-25 17.22559197480462 30.269450600723204 35.10925774719079 17.39569967728139 26-27 17.33264645561377 30.128983971773216 35.43803034573925 17.100339226873768 28-29 16.492339995334007 31.17952406874562 35.46446068901159 16.86367524690878 30-31 16.880073890428275 30.845364833989596 35.00364590928978 17.270915366292353 32-33 16.356707465269338 31.12003344254008 35.08355774185081 17.439701350339774 34-35 16.88489691172611 30.943007397470666 34.762279704101175 17.40981598670205 36-37 17.02324134646228 30.770726207023923 34.89409683408342 17.31193561243038 38-39 16.931510044610317 31.38175350613756 34.47920615019778 17.207530299054337 40-41 16.657595490329477 31.354844980075807 34.89843522208184 17.08912430751288 42-43 16.539336327054297 31.702338601504636 33.9563772088412 17.80194786259987 44-45 16.797239502332815 31.826399688958006 33.32134525660964 18.055015552099533 46-47 17.622871140835215 31.70542032817482 32.729022474531455 17.942686056458513 48-49 17.365438267344537 31.474370813932012 32.41535515354181 18.744835765181637 50-51 17.509163919921438 31.644449630040157 32.533131095100586 18.313255354937823 52-53 17.734517060648198 31.785606627835744 31.86728770213635 18.61258860937971 54-55 17.17979876537209 30.967773295095512 32.473630486560054 19.378797452972343 56-57 17.94944065935134 30.818649223920925 31.79543002653345 19.436480090194287 58-59 17.677759203825367 30.04801150721145 32.65462815379232 19.619601135170857 60-61 17.572282423830117 30.083094416638325 33.27761310073375 19.067010058797802 62-63 17.154242394790554 29.857128972689278 33.46194965497133 19.52667897754884 64-65 16.974750714327367 30.1339242327055 33.163255389041154 19.728069663925982 66-67 16.745555803940245 29.643200793103112 33.02555230495592 20.58569109800072 68-69 16.974108774248727 29.31811997045446 33.29510554756443 20.41266570773238 70-71 16.960667113741724 29.44669601811626 33.200182717633226 20.39245415050879 72-73 17.218890627581715 28.21250498119296 33.68452768571345 20.884076705511873 74-75 16.896521561652623 28.72846021517917 33.27599109737489 21.099027125793317 76-77 17.263166080211512 27.797974299655902 33.61651665079026 21.32234296934233 78-79 17.072506877411957 27.3725856152732 33.81450915206128 21.740398355253564 80-81 17.34152362809323 27.460490251346148 32.97435997123029 22.22362614933033 82-83 16.970551073962483 27.42637768490621 33.64369715229857 21.959374088832735 84-85 17.449273108917048 27.051426572339267 33.10561299852289 22.39368732022079 86-87 17.15099327450142 27.381137503401625 32.76736772538195 22.700501496715003 88-89 17.380866759323155 26.18647280078531 32.5613039294774 23.871356510414135 90-91 17.150021381642887 25.382925786261325 33.482680869260975 23.984371962834818 92-93 17.21389553080228 25.188079547442698 34.241169495149784 23.356855426605236 94-95 16.875139710956255 25.032315751620647 33.52479808729626 24.567746450126833 96-97 16.595550285275507 25.47554017670558 33.505049425075086 24.423860112943828 98-99 16.82542768273717 25.83106531881804 32.97433903576983 24.36916796267496 100-101 16.976364484528066 24.847418752915566 33.831247084434764 24.344969678121597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 259.0 1 180.0 2 118.0 3 140.5 4 121.0 5 108.0 6 160.5 7 283.5 8 401.0 9 431.0 10 323.5 11 213.5 12 204.5 13 202.0 14 201.5 15 203.0 16 192.0 17 198.5 18 221.0 19 244.0 20 262.5 21 288.0 22 323.5 23 351.5 24 396.0 25 451.5 26 560.0 27 686.0 28 837.5 29 968.0 30 1123.0 31 1305.0 32 1426.5 33 1630.0 34 1821.0 35 1877.5 36 1899.5 37 1935.0 38 1927.0 39 1849.0 40 1788.5 41 1714.0 42 1568.0 43 1545.0 44 1579.5 45 1518.0 46 1462.0 47 1612.5 48 2158.5 49 2143.5 50 1578.0 51 1197.0 52 911.5 53 803.5 54 703.5 55 589.0 56 503.0 57 431.0 58 359.5 59 304.5 60 241.5 61 179.0 62 142.5 63 92.0 64 50.0 65 38.5 66 32.0 67 25.5 68 20.0 69 15.0 70 11.0 71 8.0 72 6.5 73 4.5 74 1.5 75 1.0 76 3.5 77 3.5 78 1.0 79 0.5 80 1.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27160733339800175 2 0.23862644291395865 3 0.2347463381511301 4 0.23862644291395865 5 0.23862644291395865 6 0.2114657095741585 7 0.2172858667184014 8 0.2172858667184014 9 0.22504607624405862 10-11 0.2357163643418372 12-13 0.22310602386264428 14-15 0.21825589290910855 16-17 0.2095256571927442 18-19 0.19109515956930836 20-21 0.20661557862062274 22-23 0.19885536909496557 24-25 0.2075856048113299 26-27 0.20273547385779417 28-29 0.2114657095741585 30-31 0.22795615481618006 32-33 0.22019594529052283 34-35 0.21049568338345134 36-37 0.20661557862062274 38-39 0.19303521195072268 40-41 0.1940052381414298 42-43 0.201765447667087 44-45 0.2037055000485013 46-47 0.2114657095741585 48-49 0.21243573576486566 50-51 0.23377631196042295 52-53 0.24347657386749444 54-55 0.21825589290910855 56-57 0.19497526433213697 58-59 0.19206518576001552 60-61 0.18915510718789408 62-63 0.1940052381414298 64-65 0.1901251333786012 66-67 0.19691531671355128 68-69 0.19206518576001552 70-71 0.19303521195072268 72-73 0.19691531671355128 74-75 0.19303521195072268 76-77 0.2056455524299156 78-79 0.21049568338345134 80-81 0.1978853429042584 82-83 0.1940052381414298 84-85 0.18042487147152975 86-87 0.19206518576001552 88-89 0.19497526433213697 90-91 0.19206518576001552 92-93 0.201765447667087 94-95 0.19303521195072268 96-97 0.20079542147637985 98-99 0.2037055000485013 100-101 0.18818508099718692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 51545.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.5478707925114 #Duplication Level Percentage of deduplicated Percentage of total 1 85.74137491863131 58.77388689494616 2 8.884046075906376 12.179648850518964 3 2.6349305182124363 5.418566301290135 4 1.044349474995047 2.863517314967504 5 0.46132510684063055 1.5811426908526531 6 0.28302153793903717 1.164031428848579 7 0.20943593807488753 1.0049471335726063 8 0.14717119972829934 0.807061790668348 9 0.08773667676110151 0.5412746144145891 >10 0.4160416607703847 5.129498496459404 >50 0.053774092208417064 2.411485110097973 >100 0.028302153793903717 3.7249005723154522 >500 0.008490646138171114 4.400038801047628 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 827 1.6044233194296247 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 734 1.423998447958095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 707 1.371617033659909 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.8439227859152197 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 297 0.5761955572800466 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 188 0.36472984770588807 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 186 0.3608497429430595 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.3375691143660879 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 165 0.32010864293335917 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 148 0.28712775244931615 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 116 0.22504607624405862 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 109 0.2114657095741585 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 102 0.1978853429042584 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 96 0.18624502861577263 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.18042487147152975 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 82 0.15908429527597245 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 82 0.15908429527597245 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 76 0.14744398098748665 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 75 0.14550392860607236 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 67 0.129983509554758 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 61 0.1183431952662722 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 60 0.11640314288485788 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.11446309050344358 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 58 0.11252303812202931 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.11252303812202931 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 58 0.11252303812202931 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 56 0.1086429333592007 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 55 0.1067028809777864 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 54 0.1047628285963721 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.700261907071491E-4 0.0 24-25 0.0 0.0 0.0 0.00970026190707149 0.0 26-27 0.0 0.0 0.0 0.02037055000485013 0.0 28-29 0.0 0.0 0.0 0.04462120477252886 0.0 30-31 0.0 0.0 0.0 0.1008827238335435 0.0 32-33 0.0 0.0 0.0 0.175574740517994 0.0 34-35 0.0 0.0 0.0 0.2395964691046658 0.0 36-37 0.0 0.0 0.0 0.27936754292365895 0.0 38-39 0.0 0.0 0.0 0.3094383548355806 0.0 40-41 0.0 0.0 0.0 0.3346590357939664 0.0 42-43 0.0 0.0 0.0 0.3676399262780095 0.0 44-45 0.0 0.0 0.0 0.4074110000970026 0.0 46-47 0.0 0.0 0.0 0.4433019691531671 0.0 48-49 0.0 0.0 0.0 0.48307304297216025 0.0 50-51 0.0 0.0 0.0 0.5306043263168105 0.0 52-53 0.0 0.0 0.0 0.650887573964497 0.0 54-55 0.0 0.0 0.0 0.8555631002037055 0.0 56-57 0.0 0.0 0.0 1.1126200407411 0.0 58-59 0.0 0.0 0.0 1.4036278979532448 0.0 60-61 0.0 0.0 0.0 1.841109709962169 0.0 62-63 0.0 0.0 0.0 2.1020467552623923 0.0 64-65 0.0 0.0 0.0 2.3998447958094866 0.0 66-67 0.0 0.0 0.0 2.893588126879426 0.0 68-69 0.0 0.0 0.0 3.5250751770297795 0.0 70-71 0.0 0.0 0.0 3.997477931904162 0.0 72-73 0.0 0.0 0.0 4.537782520128044 0.0 74-75 0.0 0.0 0.0 5.477737898923271 0.0 76-77 0.0 0.0 0.0 6.216897856242118 0.0 78-79 0.0 0.0 0.0 6.793093413522165 0.0 80-81 0.0 0.0 0.0 7.274226404112911 0.0 82-83 0.0 0.0 0.0 8.028906780483073 0.0 84-85 0.0 0.0 0.0 8.928121059268602 0.0 86-87 0.0 0.0 0.0 10.022310602386264 0.0 88-89 0.0 0.0 0.0 11.67717528373266 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTTAT 20 0.0020616024 71.26023 1 CTTATAT 30 1.2885573E-4 63.342422 3 AGCTGGT 25 0.004991238 57.00818 7 GGTGATA 55 0.0025550392 34.55041 1 GTTTACT 30 0.0039316025 31.671211 78-79 GTTTTGC 30 0.003950396 31.640406 46-47 TGTTTAC 45 8.9098245E-4 26.379833 76-77 GAGTACT 480 0.0 23.753408 12-13 GTACTTT 500 0.0 22.80327 14-15 AGTACTT 455 0.0 22.448277 12-13 ACTTTTT 565 0.0 20.580261 16-17 TACTTTT 555 0.0 18.403542 14-15 GGTATCA 925 0.0 17.461964 1 GTATCAA 1120 0.0 17.390888 1 GTACATG 370 2.5393092E-9 16.691584 1 TACATGG 380 3.61797E-9 16.252333 2 ACATGGG 385 4.30191E-9 16.041262 3 AACGCAG 1210 0.0 15.704734 6 TCAACGC 1235 0.0 15.401819 4 CAACGCA 1245 0.0 15.2781105 5 >>END_MODULE