##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937325_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60834 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.804665154354474 33.0 31.0 34.0 30.0 34.0 2 31.89793536509189 33.0 31.0 34.0 30.0 34.0 3 31.87188085610021 33.0 31.0 34.0 30.0 34.0 4 35.519380609527566 37.0 35.0 37.0 33.0 37.0 5 35.53328730644048 37.0 35.0 37.0 33.0 37.0 6 35.6465463392182 37.0 35.0 37.0 35.0 37.0 7 35.595686622612355 37.0 35.0 37.0 33.0 37.0 8 35.612617943912944 37.0 35.0 37.0 33.0 37.0 9 37.20636486175494 39.0 37.0 39.0 34.0 39.0 10-11 37.17847749613703 39.0 37.0 39.0 34.0 39.0 12-13 37.089579840220935 39.0 37.0 39.0 34.0 39.0 14-15 38.183491139823126 40.0 38.0 41.0 33.5 41.0 16-17 38.129360226189306 40.0 38.0 41.0 33.5 41.0 18-19 38.144327185455495 40.0 38.0 41.0 33.5 41.0 20-21 38.12799585757964 40.0 38.0 41.0 34.0 41.0 22-23 38.04310911661242 40.0 38.0 41.0 34.0 41.0 24-25 37.90511884801262 40.0 37.5 41.0 33.5 41.0 26-27 37.67044744715126 40.0 37.0 41.0 33.0 41.0 28-29 37.491772692901996 39.5 36.5 41.0 33.0 41.0 30-31 37.32438274649045 39.0 36.0 41.0 33.0 41.0 32-33 36.993457605944045 39.0 35.0 41.0 33.0 41.0 34-35 36.75793963901765 39.0 35.0 41.0 32.5 41.0 36-37 36.59548114541211 39.0 35.0 41.0 31.5 41.0 38-39 36.4631538284512 39.0 35.0 41.0 31.0 41.0 40-41 36.17843640069698 39.0 35.0 41.0 30.5 41.0 42-43 35.812243153499686 39.0 35.0 41.0 30.0 41.0 44-45 35.391458723740016 39.0 35.0 41.0 28.0 41.0 46-47 34.77782161291383 38.5 35.0 41.0 24.0 41.0 48-49 34.22784133872506 38.5 35.0 40.0 17.5 41.0 50-51 33.182151428477496 37.5 34.0 39.5 8.5 40.5 52-53 33.19821974553703 38.0 33.5 39.5 3.5 40.5 54-55 33.43839793536509 38.0 34.0 40.0 2.0 41.0 56-57 33.14191899266857 38.0 34.0 40.0 2.0 41.0 58-59 32.86889732715258 38.0 34.0 40.0 2.0 41.0 60-61 32.52974487950817 38.0 33.0 40.0 2.0 41.0 62-63 32.145642239537096 37.5 33.0 40.0 2.0 41.0 64-65 31.73364401486011 37.0 32.0 40.0 2.0 41.0 66-67 31.282917447480028 36.5 31.5 39.5 2.0 41.0 68-69 30.758531413354376 36.0 31.0 39.0 2.0 41.0 70-71 30.305306243219253 35.5 30.5 39.0 2.0 40.0 72-73 29.817988296018676 35.0 29.5 38.5 2.0 40.0 74-75 29.35186737679587 35.0 29.0 37.5 2.0 40.0 76-77 28.88397113456291 35.0 29.0 37.0 2.0 39.0 78-79 28.414751619160338 35.0 28.0 36.5 2.0 39.0 80-81 27.950397803859683 34.0 26.5 36.0 2.0 38.5 82-83 27.494345267449123 34.0 26.0 35.5 2.0 37.0 84-85 27.006756090344215 34.0 25.0 35.0 2.0 37.0 86-87 26.56204589538745 34.0 24.5 35.0 2.0 36.5 88-89 26.171836473024953 34.0 22.5 35.0 2.0 36.0 90-91 25.7222030443502 33.5 19.5 35.0 2.0 36.0 92-93 25.281709241542558 33.0 15.0 35.0 2.0 35.0 94-95 24.787298221389356 33.0 4.5 35.0 2.0 35.0 96-97 24.244008284840714 32.5 2.0 35.0 2.0 35.0 98-99 23.623771246342507 32.0 2.0 35.0 2.0 35.0 100-101 22.069418417332415 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.4197799939879161 1101 2 -0.470638730289064 1101 3 0.030503148121866275 1101 4 -0.14830134632212122 1101 5 -0.13326287152995064 1101 6 -0.09436028150084752 1101 7 0.019699895526962052 1101 8 -0.4938212418459713 1101 9 0.15895835073921916 1101 10-11 0.10021678979621385 1101 12-13 -0.23605345285660206 1101 14-15 -0.4752184038902314 1101 16-17 -0.6005769479349823 1101 18-19 -0.7084873018341895 1101 20-21 -0.12613006831040252 1101 22-23 0.12867733097465361 1101 24-25 0.08424309773630512 1101 26-27 0.2561268840294062 1101 28-29 0.08619717705821728 1101 30-31 -0.014939237210676026 1101 32-33 0.04341183958410255 1101 34-35 0.5658316210619674 1101 36-37 0.9247401428789814 1101 38-39 0.6547170556130126 1101 40-41 1.0638932454481136 1101 42-43 0.8683554338946706 1101 44-45 0.0479368920089982 1101 46-47 0.4267588123542154 1101 48-49 -0.06304515207100536 1101 50-51 0.2221036092388431 1101 52-53 0.011658737192568935 1101 54-55 0.8777782897807995 1101 56-57 0.8538600491356263 1101 58-59 0.9110803641468337 1101 60-61 0.958809202763419 1101 62-63 1.1443910185089905 1101 64-65 1.4025247656196775 1101 66-67 0.7510762486472196 1101 68-69 1.5962618220851503 1101 70-71 1.685040492965289 1101 72-73 1.7711230821647241 1101 74-75 1.743751841790246 1101 76-77 1.4862864267640106 1101 78-79 1.8578736086533496 1101 80-81 2.5060769276437007 1101 82-83 2.689637810282246 1101 84-85 2.71308689326424 1101 86-87 2.884256417381227 1101 88-89 3.276404375836094 1101 90-91 3.368601402491983 1101 92-93 3.144314942831606 1101 94-95 2.3541926010901904 1101 96-97 2.898721948733961 1101 98-99 2.437130123831583 1101 100-101 2.0985692509132328 1102 1 0.18395773170102814 1102 2 -0.40823771666854114 1102 3 0.13598303408955914 1102 4 0.1155567468042662 1102 5 -0.066902402730463 1102 6 -0.29835141232124585 1102 7 -0.051728675901607346 1102 8 0.012830642854687824 1102 9 0.2984895512460284 1102 10-11 0.5180976893844331 1102 12-13 0.3806712858193606 1102 14-15 0.8173853338354604 1102 16-17 1.158688177183798 1102 18-19 0.8954088020619153 1102 20-21 0.3323748414140155 1102 22-23 0.17405268732562007 1102 24-25 -0.35280156491811 1102 26-27 0.48316204399139195 1102 28-29 -0.048186731715937015 1102 30-31 0.10495306560846274 1102 32-33 0.24819486144029668 1102 34-35 0.21051011330143155 1102 36-37 -0.05181988246153679 1102 38-39 0.7137129377796256 1102 40-41 1.3507953677160884 1102 42-43 1.753056099083139 1102 44-45 1.6215827583377873 1102 46-47 1.6785009726329676 1102 48-49 1.7581458520468232 1102 50-51 2.239921157544188 1102 52-53 1.9258969380161375 1102 54-55 1.8309775295654092 1102 56-57 2.1081362607289194 1102 58-59 2.1343936362405955 1102 60-61 2.058745851480559 1102 62-63 2.606142681480165 1102 64-65 1.5161611292560373 1102 66-67 1.5547664608740241 1102 68-69 1.3467369567066285 1102 70-71 1.2609828432978851 1102 72-73 -0.21786963243774338 1102 74-75 0.021468028043017284 1102 76-77 -0.642475055656007 1102 78-79 -0.5585818870704351 1102 80-81 -0.38709697162775925 1102 82-83 -0.2984046350772722 1102 84-85 -0.3263492803182757 1102 86-87 -0.48864507137391655 1102 88-89 -0.3602760169418602 1102 90-91 0.029276093654480917 1102 92-93 0.07787528492853468 1102 94-95 -0.588632866125522 1102 96-97 0.1112655027409275 1102 98-99 0.5038073490453918 1102 100-101 0.5050469195860252 1103 1 0.5280940497765378 1103 2 0.8102721396629029 1103 3 0.530236291690624 1103 4 0.2365056275259505 1103 5 0.3084734743159814 1103 6 -0.3384449639416971 1103 7 0.11950420081072366 1103 8 -0.07788101768657185 1103 9 0.7376809979550174 1103 10-11 0.33291559783749136 1103 12-13 0.3138486997852752 1103 14-15 -0.8203527216272803 1103 16-17 -0.7335604028362468 1103 18-19 0.08718962397971808 1103 20-21 0.016638122402987676 1103 22-23 0.2673537230757006 1103 24-25 0.6372585754093194 1103 26-27 -0.21638690355287338 1103 28-29 -0.18893180355021144 1103 30-31 -0.3788424572782816 1103 32-33 -0.22691372526201548 1103 34-35 -0.7436328870326818 1103 36-37 0.20778586428086498 1103 38-39 0.27293445465232224 1103 40-41 0.5334716122867462 1103 42-43 0.47607647997187286 1103 44-45 -0.038791448143996377 1103 46-47 -0.2816560604442131 1103 48-49 -0.9037318626207309 1103 50-51 -0.45144202321090177 1103 52-53 0.08735701152097874 1103 54-55 0.6499718496455955 1103 56-57 0.36097622063536505 1103 58-59 -0.08411622009084851 1103 60-61 -0.3378461966317019 1103 62-63 0.48561144192119343 1103 64-65 0.6017775676121992 1103 66-67 0.4165272360160195 1103 68-69 0.125971838096536 1103 70-71 -0.3106008519911221 1103 72-73 0.5050672202184536 1103 74-75 1.0681603100343295 1103 76-77 1.0855570191852877 1103 78-79 0.5088253965914404 1103 80-81 -0.02416680123016235 1103 82-83 -0.1950802114220771 1103 84-85 0.849895290346609 1103 86-87 0.8385350154954452 1103 88-89 -0.11035954517440416 1103 90-91 0.6872279813925353 1103 92-93 0.4807319504814913 1103 94-95 -0.6985560201515817 1103 96-97 -1.377207601168191 1103 98-99 -0.9049798210180882 1103 100-101 -0.2584846540858763 1104 1 0.5352500505943674 1104 2 0.13781251995322918 1104 3 0.7126120839621457 1104 4 0.5192698884132909 1104 5 0.7490786675280035 1104 6 0.7631702476714679 1104 7 0.6721519211133185 1104 8 0.5645233985882214 1104 9 0.6980163077076185 1104 10-11 0.9877919012519243 1104 12-13 0.702112858444039 1104 14-15 1.0324381186916654 1104 16-17 1.0483860293862008 1104 18-19 0.5073491005693782 1104 20-21 0.13961910788653142 1104 22-23 -0.2062713024814471 1104 24-25 -0.6220953399454174 1104 26-27 -0.06825768662382359 1104 28-29 -0.4281466880319229 1104 30-31 0.11696617081413052 1104 32-33 0.26603250250327193 1104 34-35 0.4654245654310287 1104 36-37 0.38975274221993317 1104 38-39 0.4580704186678659 1104 40-41 0.21983431929963615 1104 42-43 0.7849090295527432 1104 44-45 1.1811022341295612 1104 46-47 1.8254794946569888 1104 48-49 1.9268753751629575 1104 50-51 1.6784905059242448 1104 52-53 1.6693443351766746 1104 54-55 2.0818876132931123 1104 56-57 2.2762287252356472 1104 58-59 2.4092389220068853 1104 60-61 2.7141881521722198 1104 62-63 3.193237694221338 1104 64-65 3.59451933821126 1104 66-67 3.5674166246890913 1104 68-69 3.311516716444526 1104 70-71 2.5961666802108816 1104 72-73 2.1770156970125676 1104 74-75 2.0863569978282364 1104 76-77 1.3089264732846537 1104 78-79 1.6459139265444165 1104 80-81 2.0057861736216047 1104 82-83 1.809622303401067 1104 84-85 1.787229168899053 1104 86-87 1.6627018525430834 1104 88-89 2.424398173083624 1104 90-91 2.7514275315867707 1104 92-93 3.3367941054600223 1104 94-95 3.0649892671107395 1104 96-97 2.4307048911646625 1104 98-99 2.2291440800246427 1104 100-101 2.4618179425201347 1105 1 0.8475564431598421 1105 2 0.8885869496913337 1105 3 0.21697705157690095 1105 4 0.8053864752902271 1105 5 0.724860137536453 1105 6 0.11490215417668281 1105 7 0.9577052863625468 1105 8 0.7171564596977689 1105 9 0.9038738126358083 1105 10-11 0.7496899581373029 1105 12-13 0.8555907519951518 1105 14-15 1.172539498584655 1105 16-17 1.441937141748916 1105 18-19 1.3199371039487104 1105 20-21 0.9593881870191652 1105 22-23 0.8218897789040867 1105 24-25 1.154446479260443 1105 26-27 1.1813902998588617 1105 28-29 1.2275840920300354 1105 30-31 0.949407736570258 1105 32-33 1.224494907459622 1105 34-35 1.3951626673741444 1105 36-37 1.6506651612568248 1105 38-39 1.4996080137115158 1105 40-41 1.5577672959259345 1105 42-43 1.0604652109101025 1105 44-45 0.11421966583894516 1105 46-47 -0.14466975907435398 1105 48-49 -1.0577768354819383 1105 50-51 -0.7404055796854436 1105 52-53 -0.8061555240179175 1105 54-55 -0.835037196619588 1105 56-57 0.10134840983153737 1105 58-59 0.32813500761657366 1105 60-61 -0.0588335318604436 1105 62-63 -0.5451458597071337 1105 64-65 -0.7244049084798085 1105 66-67 -0.5419891764936757 1105 68-69 -0.3280214418207521 1105 70-71 -0.4581842068631836 1105 72-73 -0.040810267504472364 1105 74-75 0.06622182463758364 1105 76-77 0.5708245301700394 1105 78-79 -0.21539274763187066 1105 80-81 0.0297230927993013 1105 82-83 0.2649869894050063 1105 84-85 0.315390250284576 1105 86-87 0.0818795489666293 1105 88-89 0.6744685757870883 1105 90-91 1.2093267149154094 1105 92-93 1.6886668173721588 1105 94-95 1.5503455047902648 1105 96-97 1.6655683099199443 1105 98-99 1.9212026550739267 1105 100-101 1.402538866203603 1106 1 0.48151870731078716 1106 2 0.8527378930875606 1106 3 0.5603732779919959 1106 4 0.7539713809506026 1106 5 0.38004881678173774 1106 6 0.46018517304460715 1106 7 1.0982713240983912 1106 8 1.0902696672449324 1106 9 0.8722700390509033 1106 10-11 0.9409635430429759 1106 12-13 0.4907322614291161 1106 14-15 0.8484828948110703 1106 16-17 0.901371104013073 1106 18-19 0.3329088020619153 1106 20-21 0.6433504511701145 1106 22-23 0.7853331751304964 1106 24-25 0.02921063020384196 1106 26-27 0.567003507406028 1106 28-29 1.2323010731621125 1106 30-31 0.9512945290230945 1106 32-33 1.9018533980256578 1106 34-35 1.7260588937892365 1106 36-37 1.646655727294565 1106 38-39 1.8097495231454772 1106 40-41 1.8690880506429153 1106 42-43 1.3899463429855743 1106 44-45 2.318229099801208 1106 46-47 2.230330240925646 1106 48-49 1.9294873154614578 1106 50-51 1.7768114014466363 1106 52-53 2.3051652306990604 1106 54-55 2.8727458222483406 1106 56-57 2.8739899192654974 1106 58-59 2.348418026484495 1106 60-61 3.135270241724463 1106 62-63 2.954618291236258 1106 64-65 3.3786611292560345 1106 66-67 3.0940957291666997 1106 68-69 2.7790540298773614 1106 70-71 2.7429950384198385 1106 72-73 3.01414256268421 1106 74-75 3.5607972963356964 1106 76-77 2.9995981150757025 1106 78-79 2.9530034787832236 1106 80-81 2.7705249795917517 1106 82-83 3.1857417063861426 1106 84-85 2.9913336465109914 1106 86-87 2.9811720017968177 1106 88-89 3.7973459342776508 1106 90-91 3.3911663375569177 1106 92-93 2.867204553221214 1106 94-95 3.291854938752529 1106 96-97 2.6844362344482455 1106 98-99 1.0306366173380788 1106 100-101 1.839803017147002 1107 1 -0.9002994745073991 1107 2 -0.4836257432760789 1107 3 -0.15780854018981927 1107 4 0.21533501731423854 1107 5 -0.2631330014000852 1107 6 0.042003354862785613 1107 7 -0.23354685771978723 1107 8 0.006178758154028685 1107 9 0.0972700390509047 1107 10-11 -0.29426372968430314 1107 12-13 -0.11267682947998026 1107 14-15 0.15302834935652498 1107 16-17 0.17864383128580386 1107 18-19 -0.7182275615744445 1107 20-21 -0.1293768215571589 1107 22-23 0.17737862967594964 1107 24-25 0.15080153929474704 1107 26-27 0.45904896195148126 1107 28-29 0.4368465277075657 1107 30-31 0.5467490744776384 1107 32-33 0.45412612529838725 1107 34-35 0.9067407119710467 1107 36-37 0.7364284545672888 1107 38-39 -0.05956865867270267 1107 40-41 1.1304516870065484 1107 42-43 1.8797190702583038 1107 44-45 1.1193654634375676 1107 46-47 1.605330240925646 1107 48-49 1.2567600427341858 1107 50-51 1.8779477650829932 1107 52-53 2.10743795797179 1107 54-55 1.9897912767937882 1107 56-57 2.5194444647200456 1107 58-59 1.2097816628481368 1107 60-61 0.7932247871790068 1107 62-63 0.5307546548726236 1107 64-65 1.0161611292560373 1107 66-67 1.7770502746212493 1107 68-69 1.9631449389682665 1107 70-71 1.6054950384198357 1107 72-73 1.8766425626842071 1107 74-75 1.6755700236084259 1107 76-77 2.6064162968938795 1107 78-79 2.898458024237769 1107 80-81 2.56938861595539 1107 82-83 1.8130144336588678 1107 84-85 1.8186063737837195 1107 86-87 0.8777629108877214 1107 88-89 0.22120957064128532 1107 90-91 0.9627572466478291 1107 92-93 0.8115227350393965 1107 94-95 1.0214003932979807 1107 96-97 0.8889816889936988 1107 98-99 1.351091162792624 1107 100-101 2.3648030171470005 1108 1 -0.0574643435366724 1108 2 -0.7116688865734595 1108 3 -0.29725384065207194 1108 4 -0.11594387328668176 1108 5 0.4025064439003785 1108 6 0.30933771541748456 1108 7 0.2787797986746625 1108 8 0.07628661639747492 1108 9 0.11845647972887008 1108 10-11 -0.027892389160420805 1108 12-13 0.5884017529545389 1108 14-15 0.6031439117602275 1108 16-17 0.7140829684198522 1108 18-19 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68-69 0.16451914615643304 2311 70-71 0.06770434074541853 2311 72-73 -0.48091557685067343 2311 74-75 6.228777310504086E-4 2311 76-77 0.5350632313547692 2311 78-79 -0.14408954447259248 2311 80-81 -0.9419750204082469 2311 82-83 -0.12356061919525274 2311 84-85 0.46633364651099285 2311 86-87 0.2826254901689076 2311 88-89 -0.1651540657223478 2311 90-91 -0.6847057554663358 2311 92-93 -1.1057605630578529 2311 94-95 -0.5523311077591018 2311 96-97 -0.5364939981098935 2311 98-99 -2.418200591964247 2311 100-101 -1.925894657271602 2312 1 -0.5934812926892157 2312 2 -1.5660121069124422 2312 3 -0.3708767220080027 2312 4 0.6102213809506054 2312 5 -0.8199511832182651 2312 6 -0.26481482695539427 2312 7 -0.7392286759016073 2312 8 -0.9909803327550648 2312 9 -0.2464799609490953 2312 10-11 -0.38091145695702977 2312 12-13 -0.44364273857088676 2312 14-15 -0.9108921051889283 2312 16-17 -0.23612889598692988 2312 18-19 -0.4483411979380847 2312 20-21 0.26835045117011447 2312 22-23 -0.2771668248695036 2312 24-25 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90-91 1.147416337556919 2312 92-93 2.0828295532212167 2312 94-95 2.2074799387525275 2312 96-97 1.9031862344482455 2312 98-99 1.0400116173380773 2312 100-101 0.7398030171470005 2313 1 -1.7184812926892157 2313 2 -0.4354974010300907 2313 3 -0.7513914278903542 2313 4 -0.36220508963762654 2313 5 -0.9449511832182651 2313 6 -1.2574618857789233 2313 7 -1.5517286759016073 2313 8 -1.4376715092256518 2313 9 -1.5056711374196823 2313 10-11 -1.6024188098982037 2313 12-13 -1.2570618562179448 2313 14-15 -1.7647523993065803 2313 16-17 -1.2986288959869299 2313 18-19 -2.5604735508792587 2313 20-21 -3.2095907253004725 2313 22-23 -2.2036374131048007 2313 24-25 -1.8097599580314494 2313 26-27 -2.194025904358682 2313 28-29 -0.7088753974261195 2313 30-31 -0.2192937062710243 2313 32-33 -0.9569701313861074 2313 34-35 -1.1173234591519403 2313 36-37 -0.5643736844701479 2313 38-39 -0.9806916533251169 2313 40-41 -1.6750295964159108 2313 42-43 -0.9872595393673649 2313 44-45 -0.49427090019879216 2313 46-47 -0.3358462296625859 2313 48-49 -3.007277390420896 2313 50-51 -4.765100363259251 2313 52-53 -4.038952416359763 2313 54-55 -3.560342413045781 2313 56-57 -3.231892433675675 2313 58-59 -2.9118760911625614 2313 60-61 -4.369876817099069 2313 62-63 -5.229940532293149 2313 64-65 -5.660309458979256 2313 66-67 -5.4154630943627105 2313 68-69 -4.931240087769698 2313 70-71 -4.901857902756635 2313 72-73 -3.616004496139322 2313 74-75 -4.095820350723123 2313 76-77 -4.0754018849243 2313 78-79 -3.095525932981481 2313 80-81 -4.397857373349424 2313 82-83 -4.002493587731504 2313 84-85 -4.151313412312536 2313 86-87 -4.163680939379656 2313 88-89 -3.532801124545877 2313 90-91 -4.181627780090139 2313 92-93 -3.421766035014077 2313 94-95 -3.1904980024239435 2313 96-97 -3.3302696479046965 2313 98-99 -4.201716323838394 2313 100-101 -4.708726394617706 2314 1 -1.0774556516635734 2314 2 -0.7703390299893655 2314 3 -2.0703959527772327 2314 4 -2.113336311357088 2314 5 -2.0859768242439074 2314 6 -2.1526353397759053 2314 7 -1.6542927784657095 2314 8 -1.5078072558319846 2314 9 -2.075806884026015 2314 10-11 -2.0183313287519056 2314 12-13 -2.5217677385708868 2314 14-15 -2.2406196692914904 2314 16-17 -3.022987870345908 2314 18-19 -2.1430527363996177 2314 20-21 -2.0938290360093745 2314 22-23 -2.9246027223054014 2314 24-25 -2.3320073185141084 2314 26-27 -3.094855466952943 2314 28-29 -2.6074425165814787 2314 30-31 -1.9025516248230616 2314 32-33 -1.8468645506922883 2314 34-35 -2.8793898241594817 2314 36-37 -3.7728955547567224 2314 38-39 -3.2110838101878514 2314 40-41 -3.784758103203238 2314 42-43 -2.6199895544503207 2314 44-45 -2.5006811566090477 2314 46-47 -2.131849246253843 2314 48-49 -2.3647434537693073 2314 50-51 -2.0626116754764467 2314 52-53 -1.635860410326579 2314 54-55 -2.102574690572176 2314 56-57 -1.3657536704780924 2314 58-59 -1.9394024863360144 2314 60-61 -1.989409245455029 2314 62-63 -2.5572406831227106 2314 64-65 -2.9646081015131927 2314 66-67 -1.8068658092948375 2314 68-69 -1.69594597012264 2314 70-71 -0.8977100897852921 2314 72-73 -0.9919471809055374 2314 74-75 -1.8529847549463518 2314 76-77 -1.7612993208217382 2314 78-79 -1.6181503673706246 2314 80-81 -2.2881288665620936 2314 82-83 -3.1963095756651434 2314 84-85 -2.482384302206956 2314 86-87 -1.8133792802544662 2314 88-89 -2.6907950913633734 2314 90-91 -1.465564431673851 2314 92-93 -1.7196544211377542 2314 94-95 -1.333786086888498 2314 96-97 -0.6488970988850902 2314 98-99 -1.043722357020897 2314 100-101 -0.391607239263255 2315 1 0.2083479756034663 2315 2 0.3966403321119465 2315 3 0.06281230238224111 2315 4 0.2862884541213404 2315 5 0.140414670440272 2315 6 0.7992095632885068 2315 7 0.5702225436105905 2315 8 0.43965991114737335 2315 9 0.3106846731972439 2315 10-11 0.8900489088966239 2315 12-13 1.014817627282774 2315 14-15 0.45762923627448515 2315 16-17 0.5855174454764906 2315 18-19 0.9319941679155761 2315 20-21 1.0396919145847505 2315 22-23 1.0216136629353727 2315 24-25 1.1167106302038405 2315 26-27 0.7209669220401764 2315 28-29 1.0066913170645506 2315 30-31 0.653733553413339 2315 32-33 1.0408777882695617 2315 34-35 1.6434369425697213 2315 36-37 0.9603752394896858 2315 38-39 0.7563958646088906 2315 40-41 1.320307562838039 2315 42-43 1.1311048795709482 2315 44-45 1.0606071485816955 2315 46-47 1.7455741433646708 2315 48-49 0.8069263398517066 2315 50-51 2.0508967673002942 2315 52-53 1.2734579136258901 2315 54-55 2.032197041760533 2315 56-57 1.6995996753630536 2315 58-59 0.5978082703869418 2315 60-61 -0.13637609973895337 2315 62-63 -0.9365402453491001 2315 64-65 -1.0753022853781111 2315 66-67 -0.7684042708332974 2315 68-69 -1.403263043293375 2315 70-71 -0.17194398597040816 2315 72-73 0.3126181724403061 2315 74-75 0.0031753451161868895 2315 76-77 -0.9351579824852756 2315 78-79 -0.7232160334119015 2315 80-81 -1.3444140447984907 2315 82-83 -1.377672927760198 2315 84-85 -0.8507395242207139 2315 86-87 -0.09230360795928405 2315 88-89 -0.4212516266979591 2315 90-91 -0.5621873209796675 2315 92-93 -0.19651495897390703 2315 94-95 0.27722079241106456 2315 96-97 0.4283386734726342 2315 98-99 0.3940512514844201 2315 100-101 1.2794371634884634 2316 1 -1.1351479593558835 2316 2 -0.8194843291346636 2316 3 -2.0062933886746706 2316 4 -1.3099175079382803 2316 5 -2.889395627662708 2316 6 -1.47314816028873 2316 7 -0.9406175647904931 2316 8 -0.8902858883106219 2316 9 -0.791618849837981 2316 10-11 -1.132647568068144 2316 12-13 -1.2717677385708868 2316 14-15 -1.5376282163000425 2316 16-17 -1.5764066737647084 2316 18-19 -0.3684800868269704 2316 20-21 0.0739060067256716 2316 22-23 -0.4299446026472822 2316 24-25 -0.24440048090727373 2316 26-27 -0.21771871481619343 2316 28-29 -1.1565878157267733 2316 30-31 0.292961195689756 2316 32-33 -0.5231466019743394 2316 34-35 -1.0225522173218806 2316 36-37 -0.7825109393721092 2316 38-39 0.05280507870103435 2316 40-41 0.02186582842068674 2316 42-43 -0.9864425459033086 2316 44-45 -1.7442709001987922 2316 46-47 -2.089114203518797 2316 48-49 -1.3455126845385408 2316 50-51 -0.1384663763311451 2316 52-53 -1.4948347693009367 2316 54-55 -1.7425319555294436 2316 56-57 -1.5676767474011655 2316 58-59 -2.1210264179599463 2316 60-61 -1.5994519804977614 2316 62-63 -1.0967705976526254 2316 64-65 -1.719949981855077 2316 66-67 -2.1434042708332974 2316 68-69 -1.9598348590115293 2316 70-71 -1.630616072691275 2316 72-73 -1.6511352150935714 2316 74-75 -1.3155915925531865 2316 76-77 -1.2420685515909682 2316 78-79 -0.7719965212167779 2316 80-81 -1.3308639092971326 2316 82-83 -1.178147182502748 2316 84-85 -1.8253330201556714 2316 86-87 -1.0868835537587422 2316 88-89 -1.429042954611237 2316 90-91 -1.6546669957764166 2316 92-93 -1.7869621134454476 2316 94-95 -2.6970339501363583 2316 96-97 -3.5655637655517545 2316 98-99 -3.5554744937730334 2316 100-101 -2.5935303161863352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 357.0 3 42.0 4 43.0 5 41.0 6 41.0 7 58.0 8 75.0 9 92.0 10 102.0 11 164.0 12 251.0 13 417.0 14 505.0 15 724.0 16 896.0 17 1320.0 18 1121.0 19 722.0 20 699.0 21 637.0 22 715.0 23 731.0 24 703.0 25 783.0 26 738.0 27 818.0 28 862.0 29 1054.0 30 1205.0 31 1485.0 32 1912.0 33 2404.0 34 3318.0 35 5303.0 36 6853.0 37 10943.0 38 10855.0 39 1845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.455723755812805 22.37063421391115 16.104350120378616 19.06929190989743 2 14.758584310040717 23.286022781596692 40.36562649390898 21.58976641445362 3 18.26319173136838 28.795146959431616 32.693734236684634 20.247927072515374 4 11.35966502917807 20.27298803204642 39.360720055388875 29.006626883386634 5 10.485280058022616 39.984505324234334 35.58170968911746 13.948504928625589 6 25.754905029405467 39.79869199215854 18.287399304811952 16.15900367362404 7 21.725092250922508 38.155640484976274 23.059435951502373 17.059831312598842 8 23.220757825370676 34.77594728171334 21.373970345963755 20.629324546952223 9 24.40736042699702 18.15725746668204 23.62980412829679 33.80557797802415 10-11 21.909006285370655 29.12688540525401 27.898643249610767 21.065465059764566 12-13 24.559712680604292 28.4922321619796 28.344783274848023 18.60327188256808 14-15 21.631950450116545 29.64097749005461 29.41283058651051 19.314241473318344 16-17 17.354343940230148 32.78198337712831 30.21276946268091 19.650903219960625 18-19 16.667215761209764 30.87503706388166 34.45293710671103 18.004810068197543 20-21 18.216936141472214 29.656779975125403 34.39366109596488 17.732622787437506 22-23 17.513631047490406 29.60778822870509 34.48284382361177 18.39573690019273 24-25 17.701512942784902 30.379924064602736 34.356237491661105 17.56232550095125 26-27 17.484164833988157 30.11440855963824 35.1034124885715 17.298014117802104 28-29 16.8123151938457 30.96589271153355 35.189562724959025 17.032229369661728 30-31 17.10555578435814 31.075326386886864 34.433507680902764 17.38561014785223 32-33 16.664195578399394 31.323514875951368 34.44120457316069 17.571084972488553 34-35 17.34228298468127 31.25020589688684 33.97875144127821 17.428759677153682 36-37 17.34503945049334 30.749971173961026 34.45205817918266 17.45293119636298 38-39 17.35678756135323 31.230029317785025 33.89333596863985 17.519847152221892 40-41 16.969462379760177 31.498715245750432 34.400942153116354 17.13088022137304 42-43 16.86980153174669 31.89327184386066 33.07584616651569 18.161080457876967 44-45 17.342778304023724 31.64779045344096 32.80260285820189 18.206828384333427 46-47 17.912500617700836 31.5991039219885 32.24151279052529 18.246882669785368 48-49 17.862908539799687 31.48309831312599 32.20545598313126 18.44853716394307 50-51 17.60233918128655 31.81039453092826 32.18433407462318 18.402932213162014 52-53 17.708745222090418 32.03917885857388 31.56303545538421 18.689040463951496 54-55 17.521663207143092 31.239497874864092 31.898454746136867 19.340384171855952 56-57 18.078212106489676 30.965340623687617 31.498118396890618 19.45832887293209 58-59 17.856995842423743 30.62939941546948 32.11295434898942 19.40065039311736 60-61 17.81362184237394 30.44330270394888 32.65899285314363 19.084082600533545 62-63 17.406699825422443 30.548601732599888 32.93257353667776 19.11212490529991 64-65 17.236183387128648 30.78519201633621 32.52173769843884 19.456886898096304 66-67 17.359401121661573 29.963681882942016 32.5001852965156 20.176731698880808 68-69 17.349736900615135 29.709231947429533 32.934772762831756 20.00625838912358 70-71 17.590968602554284 29.84363034510017 32.55272020618726 20.01268084615828 72-73 17.487769926372486 28.67355174515327 33.324274019535174 20.51440430893907 74-75 17.140927547213323 29.322088340183004 32.70629318793908 20.830690924664587 76-77 17.459663803256547 28.60884390159533 33.00691007025375 20.924582224894372 78-79 17.187023875213097 28.47648303863354 33.073635143259516 21.262857942893852 80-81 17.608695652173914 28.24769433465086 32.462944664031625 21.68066534914361 82-83 17.331828962972722 28.17647252602451 33.149953880616685 21.341744630386085 84-85 17.62303518349266 28.038106530313133 32.5799306704872 21.758927615707005 86-87 17.49388541640932 28.098261564180483 32.11288715402162 22.294965865388576 88-89 17.752283377669432 27.061216758221395 31.890694360942508 23.29580550316667 90-91 17.663141944197385 26.330829792140197 32.985802286128404 23.02022597753401 92-93 17.77045669810155 26.314705761232137 33.32784252357616 22.586995017090146 94-95 17.277586079107955 26.303436576106204 32.79200191058149 23.62697543420435 96-97 16.87817572408568 26.18320170634692 33.11153019459931 23.82709237496809 98-99 17.046895847403185 26.27369912204121 33.01652143833698 23.662883592218616 100-101 16.916565801282843 25.894820047921353 33.28392988003195 23.90468427076386 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 383.0 1 262.5 2 153.0 3 158.5 4 133.0 5 137.5 6 210.5 7 361.0 8 491.5 9 494.5 10 379.5 11 285.0 12 263.5 13 247.0 14 236.5 15 219.0 16 215.0 17 220.5 18 239.5 19 254.5 20 289.0 21 343.0 22 359.5 23 405.5 24 444.5 25 485.5 26 637.5 27 825.5 28 954.0 29 1092.5 30 1255.5 31 1427.0 32 1660.0 33 1849.0 34 2044.0 35 2216.5 36 2285.5 37 2291.5 38 2225.0 39 2160.5 40 2073.0 41 2002.5 42 1937.0 43 1870.5 44 1786.5 45 1784.0 46 1819.5 47 1945.0 48 2670.5 49 2658.0 50 1818.0 51 1370.5 52 1083.0 53 955.5 54 868.0 55 745.5 56 612.0 57 526.5 58 454.0 59 358.0 60 288.0 61 228.0 62 160.0 63 97.0 64 75.0 65 56.0 66 31.5 67 29.5 68 31.0 69 23.0 70 16.0 71 10.5 72 8.5 73 7.5 74 6.5 75 5.5 76 5.0 77 4.0 78 4.0 79 3.0 80 1.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31561297958378537 2 0.28109280994180885 3 0.28109280994180885 4 0.28273662754380774 5 0.27616135713581225 6 0.21534010586185354 7 0.21369628825985468 8 0.22027155866785023 9 0.21534010586185354 10-11 0.22602492027484633 12-13 0.22191537626984909 14-15 0.2095867442548575 16-17 0.22109346746884964 18-19 0.2104086530558569 20-21 0.21287437945885523 22-23 0.20876483545385804 24-25 0.20465529144886083 26-27 0.21451819706085415 28-29 0.21123056185685637 30-31 0.216162014662853 32-33 0.21698392346385245 34-35 0.2038333826478614 36-37 0.20547720024986027 38-39 0.19725811223986586 40-41 0.20054574744386364 42-43 0.195614294637867 44-45 0.216162014662853 46-47 0.20547720024986027 48-49 0.21369628825985468 50-51 0.21205247065785582 52-53 0.22684682907584572 54-55 0.21698392346385245 56-57 0.188217115428872 58-59 0.16684748660288654 60-61 0.17753230101587927 62-63 0.19068284183187034 64-65 0.18081993621987705 66-67 0.1980800210408653 68-69 0.1898609330308709 70-71 0.18492948022487424 72-73 0.2021895650458625 74-75 0.20629910905085974 76-77 0.20629910905085974 78-79 0.20136765624486308 80-81 0.18739520662787257 82-83 0.20054574744386364 84-85 0.1799980274188776 86-87 0.19479238583686753 88-89 0.20301147384686197 90-91 0.19725811223986586 92-93 0.2079429266528586 94-95 0.19643620343886642 96-97 0.19643620343886642 98-99 0.20547720024986027 100-101 0.1799980274188776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 60834.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.09077160798238 #Duplication Level Percentage of deduplicated Percentage of total 1 84.84098593619836 56.920472104415296 2 9.371784191698927 12.57520465529145 3 2.665751947861028 5.365420652924351 4 1.0976625667663058 2.9457211427819967 5 0.6541872886754545 2.194496498668508 6 0.330768853824668 1.3314922576190946 7 0.24991424511197138 1.173685767827202 8 0.15190865879355123 0.8153335305914456 9 0.08820502768657813 0.5325969030476378 >10 0.4581761160386142 5.3917217345563335 >50 0.053903072475131084 2.47065785580432 >100 0.026951536237565542 3.1774994246638393 >500 0.009800558631842015 5.105697471808528 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 1.5008054706249794 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 882 1.4498471249630143 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 808 1.3282046224150967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 503 0.8268402538054378 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 317 0.5210901798336456 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 267 0.43889929973370156 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 207 0.3402702436137686 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 207 0.3402702436137686 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 201 0.3304073380017753 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 135 0.22191537626984909 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 134 0.22027155866785023 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.21698392346385245 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 127 0.20876483545385804 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.17095703060788375 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 102 0.16766939540388598 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 98 0.16109412499589046 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 84 0.13808067856790612 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 83 0.13643686096590724 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 83 0.13643686096590724 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 81 0.13314922576190946 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 81 0.13314922576190946 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 78 0.12821777295591283 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 78 0.12821777295591283 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.111779596935924 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 67 0.11013577933392511 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 64 0.10520432652792845 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 62 0.1019166913239307 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 62 0.1019166913239307 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 62 0.1019166913239307 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 61 0.10027287372193182 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0016438176019988823 0.0 22-23 0.0 0.0 0.0 0.008219088009994411 0.0 24-25 0.0 0.0 0.0 0.01479435841798994 0.0 26-27 0.0 0.0 0.0 0.023835355228983793 0.0 28-29 0.0 0.0 0.0 0.049314528059966466 0.0 30-31 0.0 0.0 0.0 0.10931387053292566 0.0 32-33 0.0 0.0 0.0 0.17424466581188153 0.0 34-35 0.0 0.0 0.0 0.25314791070782783 0.0 36-37 0.0 0.0 0.0 0.2909557155538022 0.0 38-39 0.0 0.0 0.0 0.31643488838478484 0.0 40-41 0.0 0.0 0.0 0.34355787881776634 0.0 42-43 0.0 0.0 0.0 0.3788999572607423 0.0 44-45 0.0 0.0 0.0 0.41177630930071996 0.0 46-47 0.0 0.0 0.0 0.46191274616168587 0.0 48-49 0.0 0.0 0.0 0.5120491830226518 0.0 50-51 0.0 0.0 0.0 0.5613637110826182 0.0 52-53 0.0 0.0 0.0 0.689581484038531 0.0 54-55 0.0 0.0 0.0 0.8375250682184305 0.0 56-57 0.0 0.0 0.0 1.0405365420652926 0.0 58-59 0.0 0.0 0.0 1.2936844527731204 0.0 60-61 0.0 0.0 0.0 1.6569681428148733 0.0 62-63 0.0 0.0 0.0 1.8813492454877205 0.0 64-65 0.0 0.0 0.0 2.1328533385935495 0.0 66-67 0.0 0.0 0.0 2.6309300719992113 0.0 68-69 0.0 0.0 0.0 3.2169510471118126 0.0 70-71 0.0 0.0 0.0 3.6361245356215273 0.0 72-73 0.0 0.0 0.0 4.061873294539238 0.0 74-75 0.0 0.0 0.0 4.928165170792649 0.0 76-77 0.0 0.0 0.0 5.477200249860276 0.0 78-79 0.0 0.0 0.0 5.842949666305026 0.0 80-81 0.0 0.0 0.0 6.215274353157774 0.0 82-83 0.0 0.0 0.0 6.950060821251274 0.0 84-85 0.0 0.0 0.0 7.727586546996745 0.0 86-87 0.0 0.0 0.0 8.63004241049413 0.0 88-89 0.0 0.0 0.0 10.097149620278135 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACT 20 0.0020541744 71.34089 4 TCTAAGC 20 0.0020541744 71.34089 3 ATGGGGA 70 2.0252177E-4 33.94387 5 CATGGGG 130 7.916242E-9 32.926563 4 TATTCAT 70 0.008278496 27.177483 2 TACATGG 335 0.0 25.554947 2 GTACATG 340 0.0 25.179138 1 ACATGGG 365 0.0 23.45454 3 AGTACTT 535 0.0 22.631754 12-13 GAGTACT 560 0.0 22.469305 12-13 GTACTTT 555 0.0 22.243963 14-15 TACTTTT 605 0.0 20.013203 14-15 CATGGGC 120 0.0046779783 19.816916 4 ACTTTTT 630 0.0 19.595873 16-17 GTATCAA 1200 0.0 18.627901 1 GACGATC 195 1.4551915E-11 18.262424 94-95 AGTACAT 255 5.2709092E-6 16.786093 2 ATCAACG 1285 0.0 16.65546 3 CCGACGA 200 4.1836756E-10 16.618807 92-93 AACGCAG 1290 0.0 16.577236 6 >>END_MODULE