##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937325_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60834 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.220189367787754 33.0 31.0 34.0 31.0 34.0 2 32.416576256698555 34.0 31.0 34.0 31.0 34.0 3 32.45185258243745 34.0 31.0 34.0 31.0 34.0 4 36.08773054541868 37.0 35.0 37.0 35.0 37.0 5 36.00918894039518 37.0 35.0 37.0 35.0 37.0 6 36.12300687115758 37.0 35.0 37.0 35.0 37.0 7 36.08011966992142 37.0 35.0 37.0 35.0 37.0 8 36.08488674096722 37.0 35.0 37.0 35.0 37.0 9 37.761449189597926 39.0 37.0 39.0 35.0 39.0 10-11 37.72062497945228 39.0 37.0 39.0 35.0 39.0 12-13 37.65494624716442 39.0 37.0 39.0 35.0 39.0 14-15 38.78352565999277 40.0 38.0 41.0 35.0 41.0 16-17 38.747715093533216 40.0 38.0 41.0 35.0 41.0 18-19 38.80052273399744 40.0 38.0 41.0 35.0 41.0 20-21 38.77011210836046 40.0 38.0 41.0 35.0 41.0 22-23 38.66092152414768 40.0 38.0 41.0 35.0 41.0 24-25 38.51983265936812 40.0 38.0 41.0 34.5 41.0 26-27 38.35474405759937 40.0 37.5 41.0 34.0 41.0 28-29 38.209677154222966 40.0 37.0 41.0 34.0 41.0 30-31 38.083933326758064 40.0 37.0 41.0 34.0 41.0 32-33 37.802643258704016 40.0 35.5 41.0 33.5 41.0 34-35 37.60330571719762 40.0 35.0 41.0 33.0 41.0 36-37 37.336686721241406 40.0 35.0 41.0 33.0 41.0 38-39 37.115790511884796 40.0 35.0 41.0 33.0 41.0 40-41 36.8402127099977 39.0 35.0 41.0 33.0 41.0 42-43 36.45107998816451 39.0 35.0 41.0 31.5 41.0 44-45 35.95208271690173 39.0 35.0 41.0 30.5 41.0 46-47 35.249646579215565 39.0 35.0 41.0 26.5 41.0 48-49 34.69604168721439 39.0 35.0 41.0 22.0 41.0 50-51 34.30471446888254 39.0 35.0 41.0 17.0 41.0 52-53 33.99938356839925 39.0 35.0 40.5 7.0 41.0 54-55 33.69672387151921 38.0 34.5 40.0 2.0 41.0 56-57 33.384965644212116 38.0 34.0 40.0 2.0 41.0 58-59 33.070314297925506 38.0 34.0 40.0 2.0 41.0 60-61 32.67984186474669 38.0 33.0 40.0 2.0 41.0 62-63 32.248200019725815 37.0 33.0 40.0 2.0 41.0 64-65 31.853930367886377 37.0 32.5 40.0 2.0 41.0 66-67 31.38263142321728 36.0 32.0 39.0 2.0 41.0 68-69 30.945573199197817 36.0 31.0 39.0 2.0 40.5 70-71 30.464477101620805 35.0 31.0 39.0 2.0 40.0 72-73 29.981794720057863 35.0 30.5 38.0 2.0 40.0 74-75 29.48441660913305 35.0 29.5 37.0 2.0 39.5 76-77 28.19479238583687 33.5 28.0 36.0 2.0 38.5 78-79 28.650039451622447 34.0 29.0 36.0 2.0 39.0 80-81 28.42223098924943 34.0 29.0 36.0 2.0 38.5 82-83 28.033829766249138 34.0 28.5 35.5 2.0 37.0 84-85 27.607842653779137 34.0 27.5 35.0 2.0 37.0 86-87 27.22340303119966 34.0 26.5 35.0 2.0 36.0 88-89 26.7921885787553 34.0 25.5 35.0 2.0 36.0 90-91 26.358048130979387 34.0 24.5 35.0 2.0 35.5 92-93 26.01186014399842 33.5 23.5 35.0 2.0 35.0 94-95 25.55763224512608 33.0 19.5 35.0 2.0 35.0 96-97 25.111738501495875 33.0 12.5 35.0 2.0 35.0 98-99 24.751060262353292 33.0 2.0 35.0 2.0 35.0 100-101 23.211583982641287 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1491320230241655 1101 2 -0.6517018130544976 1101 3 -0.14912945160546087 1101 4 -0.17714484161916033 1101 5 -0.516700360198783 1101 6 -0.629112842300799 1101 7 -0.1391862328381137 1101 8 -0.34869733618762666 1101 9 -0.15869064127732457 1101 10-11 -0.3599047341601178 1101 12-13 -0.4131971239356247 1101 14-15 -0.17308332287284145 1101 16-17 -0.6429239755244396 1101 18-19 -0.3015388112886228 1101 20-21 -0.29172627949014895 1101 22-23 -0.9080103368352681 1101 24-25 -0.5183920727032714 1101 26-27 -1.4714338214536937 1101 28-29 -1.467104538102916 1101 30-31 -0.6338569720278784 1101 32-33 -0.9155518877410174 1101 34-35 -0.8248609685836783 1101 36-37 -0.5891224107194333 1101 38-39 -1.1421556360993748 1101 40-41 -1.0891898706500243 1101 42-43 -0.5221514922588923 1101 44-45 -0.5803604769046302 1101 46-47 -0.15212222369041228 1101 48-49 -0.16063429881125302 1101 50-51 0.030292922972449787 1101 52-53 0.8921923966495484 1101 54-55 1.0195526512531288 1101 56-57 0.7733721894591525 1101 58-59 0.6291922880231979 1101 60-61 1.4819195123429978 1101 62-63 1.5838464010186364 1101 64-65 0.5121981887959528 1101 66-67 0.49306745502954286 1101 68-69 0.7385673685764793 1101 70-71 1.2569159917615842 1101 72-73 0.7803954689096884 1101 74-75 -0.06389513758377419 1101 76-77 0.6785982758555207 1101 78-79 1.031054599173082 1101 80-81 1.2121129348086583 1101 82-83 1.0392715938455943 1101 84-85 0.9971272008114589 1101 86-87 1.3055631884812975 1101 88-89 1.9405169447674098 1101 90-91 2.022001794088691 1101 92-93 2.1897057416085595 1101 94-95 2.8281519107635518 1101 96-97 2.071891641231346 1101 98-99 1.5735452301383184 1101 100-101 1.3645674318805128 1102 1 0.13091852920091895 1102 2 -0.08708350453375147 1102 3 -0.22673477311322188 1102 4 0.09906675166560319 1102 5 0.1621086356833885 1102 6 0.25463755364472007 1102 7 -0.48967720528030867 1102 8 -0.23529853986200067 1102 9 0.06256371412337103 1102 10-11 -0.16414927011197733 1102 12-13 -0.20952274952954042 1102 14-15 0.29048968948065834 1102 16-17 -0.24563224286684715 1102 18-19 -0.11979981857402322 1102 20-21 -0.1727050568103934 1102 22-23 -0.07589123642349449 1102 24-25 0.04297948257072193 1102 26-27 -0.21589058420313734 1102 28-29 0.2552584647478824 1102 30-31 0.010742331108005487 1102 32-33 0.03867681039012183 1102 34-35 0.33003291801752965 1102 36-37 0.2574091065437898 1102 38-39 0.6828364449902651 1102 40-41 0.6469520362235954 1102 42-43 0.37521942950227327 1102 44-45 0.04349128109347333 1102 46-47 0.4504276654448418 1102 48-49 0.7410920236467717 1102 50-51 0.8845057832828687 1102 52-53 0.845945960159213 1102 54-55 0.7046967754216453 1102 56-57 1.0719942990568754 1102 58-59 0.5595850659769539 1102 60-61 0.12810259755047326 1102 62-63 -0.09044881595950827 1102 64-65 -0.30170741779258137 1102 66-67 -0.6835242459524018 1102 68-69 -0.03495179518658631 1102 70-71 -0.008367505229227135 1102 72-73 -0.7307701946950012 1102 74-75 -0.9972179123699618 1102 76-77 -0.9868277615217345 1102 78-79 -0.05898341159663545 1102 80-81 -0.694602301174875 1102 82-83 -0.6659865626320745 1102 84-85 -0.7142285166418212 1102 86-87 -0.8457671884588329 1102 88-89 -0.926682928530024 1102 90-91 -1.0088819563072526 1102 92-93 -1.228967049015452 1102 94-95 -0.9141667461892489 1102 96-97 -1.5940095307673872 1102 98-99 -1.1913423213345986 1102 100-101 -1.5386634835455233 1103 1 0.16549921747355256 1103 2 0.14228502202822568 1103 3 -0.051993710634107515 1103 4 -0.17056238298190607 1103 5 0.09795895309066793 1103 6 -0.46437347208197366 1103 7 -0.25162174253389935 1103 8 -0.06155981617339279 1103 9 0.10549722565026798 1103 10-11 -0.07828224705817632 1103 12-13 -0.11814286312793598 1103 14-15 -0.0992530435631096 1103 16-17 0.15622208389559944 1103 18-19 0.14473409388838832 1103 20-21 -0.10279192617223742 1103 22-23 0.42778400580838394 1103 24-25 0.18673157077653002 1103 26-27 -0.4490178812295298 1103 28-29 0.382388434677722 1103 30-31 0.06085927063356422 1103 32-33 0.19270287119867646 1103 34-35 0.3332069908541513 1103 36-37 0.6296109107536196 1103 38-39 0.9534679184282879 1103 40-41 0.32052370344710823 1103 42-43 -0.11074782743056488 1103 44-45 0.14121931316831393 1103 46-47 0.6392849992236549 1103 48-49 0.8838417730620804 1103 50-51 0.5078769827482859 1103 52-53 0.4364905642353847 1103 54-55 0.16961491775375492 1103 56-57 -0.0675813775218117 1103 58-59 0.3794146683825659 1103 60-61 0.3532947124853223 1103 62-63 0.06650073298803605 1103 64-65 0.023050350333047476 1103 66-67 -0.10851589312914811 1103 68-69 0.035044863684110084 1103 70-71 0.40626664111223576 1103 72-73 0.43837447620376224 1103 74-75 0.14878943811450185 1103 76-77 0.028624961498646684 1103 78-79 0.3325134143305277 1103 80-81 -0.10322241477327054 1103 82-83 -0.1653183367717368 1103 84-85 0.04596192772837426 1103 86-87 0.12374500666311761 1103 88-89 -0.056903443063937686 1103 90-91 -0.5461355480212511 1103 92-93 0.5262117014021896 1103 94-95 0.8267286764636061 1103 96-97 0.7346740642877414 1103 98-99 0.8595263722838453 1103 100-101 1.2830872682085719 1104 1 0.20863967954674933 1104 2 0.4886354616111319 1104 3 0.5632179025329407 1104 4 0.2960088703405219 1104 5 0.3739761274926323 1104 6 0.23752797956390737 1104 7 0.28589614746815073 1104 8 0.43343098325412655 1104 9 0.26988078729410603 1104 10-11 0.3474998016026234 1104 12-13 0.16288351184650907 1104 14-15 0.3862304976350046 1104 16-17 0.23534700722416346 1104 18-19 -0.17922828599302676 1104 20-21 0.13335610081610838 1104 22-23 0.5327727606642441 1104 24-25 0.2716835233424746 1104 26-27 0.6717140754255482 1104 28-29 0.34681288775480823 1104 30-31 0.3405566740275603 1104 32-33 0.37558980638575434 1104 34-35 0.42286145824323285 1104 36-37 0.599964621651182 1104 38-39 -0.19885267040834975 1104 40-41 0.4074179990921749 1104 42-43 0.7738361022361673 1104 44-45 0.8254352199358479 1104 46-47 1.0296959581277676 1104 48-49 1.0202147029332664 1104 50-51 0.9468465186305224 1104 52-53 0.6994224799990363 1104 54-55 1.155916287616769 1104 56-57 1.3931628465632429 1104 58-59 1.0831562345244592 1104 60-61 1.4823071843724662 1104 62-63 1.2119720067561808 1104 64-65 2.2208626586544504 1104 66-67 1.7014411708659445 1104 68-69 2.273748605250251 1104 70-71 1.7346248500674584 1104 72-73 1.703248735046536 1104 74-75 0.8699098633854057 1104 76-77 1.635760516599852 1104 78-79 1.9604013718034352 1104 80-81 1.610639781096694 1104 82-83 1.579007976865558 1104 84-85 1.320445673383663 1104 86-87 0.0715062006929692 1104 88-89 0.8094837988088912 1104 90-91 1.1274485861026484 1104 92-93 0.2369048111956893 1104 94-95 -0.7652770483370475 1104 96-97 -0.029484594473245807 1104 98-99 0.31894162478844734 1104 100-101 1.3590431054606675 1105 1 0.7560911016813634 1105 2 0.702378069327267 1105 3 0.7856904454785862 1105 4 0.29234792976040325 1105 5 0.22331434208007295 1105 6 0.3738276134698495 1105 7 0.3467240832608809 1105 8 -0.22103254814548023 1105 9 0.5906355042752409 1105 10-11 0.36369242339929997 1105 12-13 0.3836663899090311 1105 14-15 0.3804574759509691 1105 16-17 1.0273774211920568 1105 18-19 1.3582259522497253 1105 20-21 0.6847500743446986 1105 22-23 1.376478758974578 1105 24-25 0.9314976602053235 1105 26-27 1.22324885436106 1105 28-29 0.9117241803484433 1105 30-31 0.8620999012874861 1105 32-33 1.3564402710435957 1105 34-35 1.2361534886572016 1105 36-37 1.1430510761710337 1105 38-39 1.261299399431131 1105 40-41 1.6080657039640442 1105 42-43 0.9909580397185636 1105 44-45 1.6659026938868422 1105 46-47 2.3315827505806013 1105 48-49 1.8106962574249579 1105 50-51 1.8073083603652407 1105 52-53 1.9280904608494964 1105 54-55 2.089878551767711 1105 56-57 2.295303088748547 1105 58-59 2.3291432804270187 1105 60-61 2.5415862606889945 1105 62-63 3.072574653897835 1105 64-65 3.4415047313100438 1105 66-67 3.125265445718103 1105 68-69 3.1981361017301175 1105 70-71 3.1232477731877104 1105 72-73 2.6614295291890286 1105 74-75 2.740932110639701 1105 76-77 2.4108942817364323 1105 78-79 2.6638651848138544 1105 80-81 3.011794385433504 1105 82-83 2.7447359408193748 1105 84-85 2.891270793068255 1105 86-87 3.2841223651536815 1105 88-89 3.6222126075951486 1105 90-91 3.477772438538583 1105 92-93 3.7442266923559266 1105 94-95 4.269220276360219 1105 96-97 4.049225627999295 1105 98-99 3.635049763340966 1105 100-101 4.172335135874633 1106 1 4.3072432286805906E-4 1106 2 -0.31696155331423626 1106 3 -0.5218567243327357 1106 4 0.2527252882509643 1106 5 0.16576717226875104 1106 6 0.014393651205693914 1106 7 0.13776181910994012 1106 8 0.2860429235526354 1106 9 0.26988078729410603 1106 10-11 0.037984876229486986 1106 12-13 -0.585437383675874 1106 14-15 -0.5704859202754378 1106 16-17 -0.027339559940017466 1106 18-19 -0.08351933076914975 1106 20-21 -0.36477822754209654 1106 22-23 0.16374290991797835 1106 24-25 0.37560143379023714 1106 26-27 0.7569752694554026 1106 28-29 0.5921487086503276 1106 30-31 7.159940069101367E-5 1106 32-33 0.2587987616096399 1106 34-35 0.3653987716760696 1106 36-37 -0.006005527602546579 1106 38-39 0.3877144937707513 1106 40-41 0.42103740207724627 1106 42-43 -0.20526837537577336 1106 44-45 0.025808354264206912 1106 46-47 -0.3203040418722338 1106 48-49 0.4651773895004325 1106 50-51 0.839383832063362 1106 52-53 0.8837508382079875 1106 54-55 1.2309162876167719 1106 56-57 0.4707747868617531 1106 58-59 0.32513384646475885 1106 60-61 0.6793221097455913 1106 62-63 0.8254048425770755 1106 64-65 0.7766462407440038 1106 66-67 1.0969635589256477 1106 68-69 1.1278530828621953 1106 70-71 1.7062666411122365 1106 72-73 0.9847785857928031 1106 74-75 0.7296113559227173 1106 76-77 0.6906112628685079 1106 78-79 1.1419312225497045 1106 80-81 1.4419830646787872 1106 82-83 0.8980378276118266 1106 84-85 1.0808934345776926 1106 86-87 0.8237450066631169 1106 88-89 1.605939022689487 1106 90-91 0.24050828759518694 1106 92-93 0.6484719753747932 1106 94-95 -0.7833740632624249 1106 96-97 -0.8238875795478755 1106 98-99 0.6543893859824763 1106 100-101 1.2890804188935014 1107 1 -0.1722965484044039 1107 2 0.06258390123121416 1107 3 0.3054160029399924 1107 4 0.13454347006914702 1107 5 0.2430398995414791 1107 6 0.023484560296601842 1107 7 0.026398182746305565 1107 8 -0.24804798553827112 1107 9 0.17897169638501254 1107 10-11 -0.022242396497780703 1107 12-13 0.09978988905138664 1107 14-15 -0.3386677384572536 1107 16-17 0.2726604400599797 1107 18-19 -0.12783751258732678 1107 20-21 -0.13750550026936992 1107 22-23 0.35010654628161575 1107 24-25 0.3631014337902343 1107 26-27 -0.09643382145369372 1107 28-29 0.06373961774123416 1107 30-31 0.38075341758250403 1107 32-33 0.05198057979145432 1107 34-35 0.9153987716760668 1107 36-37 0.7258126542156305 1107 38-39 0.5558963119525657 1107 40-41 1.2744464929863426 1107 42-43 1.3220043518969504 1107 44-45 2.114444717900561 1107 46-47 2.7796959581277676 1107 48-49 3.8231319349549793 1107 50-51 3.3371111047906297 1107 52-53 3.004205383662537 1107 54-55 2.8945526512531288 1107 56-57 1.643502059589025 1107 58-59 1.8467247555556625 1107 60-61 1.4202312006546904 1107 62-63 2.070859388031625 1107 64-65 1.9732371498349117 1107 66-67 1.9492362861983743 1107 68-69 1.9528530828621946 1107 70-71 1.5880848229304156 1107 72-73 0.9995513130655311 1107 74-75 1.1568840831954468 1107 76-77 1.3247021719594159 1107 78-79 1.393067586186067 1107 80-81 1.7056194283151527 1107 82-83 1.5798560094300065 1107 84-85 1.3104388891231444 1107 86-87 1.771472279390391 1107 88-89 0.9388935681440351 1107 90-91 1.1177810148679121 1107 92-93 0.9689265208293385 1107 94-95 1.172307754919398 1107 96-97 0.9306578749975785 1107 98-99 1.002116658709749 1107 100-101 2.147034964348048 1108 1 -0.2682133434737395 1108 2 0.13346217549931794 1108 3 -0.20151774128189004 1108 4 0.139165966217071 1108 5 0.13441124006536143 1108 6 0.10168178679891327 1108 7 -0.20588224868667027 1108 8 0.14833105914585332 1108 9 -0.001305653383859351 1108 10-11 -0.25798970004169064 1108 12-13 -0.1475136548623226 1108 14-15 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-2.949943636429417 2316 74-75 -2.653721977410612 2316 76-77 -2.5704998482426014 2316 78-79 -2.5816798885614105 2316 80-81 -3.033016935321214 2316 82-83 -3.199184394610395 2316 84-85 -3.7552176765334195 2316 86-87 -3.2095883266702145 2316 88-89 -3.078783199532733 2316 90-91 -2.512269490182593 2316 92-93 -3.7015280246252082 2316 94-95 -3.826429618817979 2316 96-97 -3.412776468436764 2316 98-99 -3.22894394735086 2316 100-101 -2.3845306922176093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 4.0 6 4.0 7 10.0 8 17.0 9 49.0 10 90.0 11 166.0 12 259.0 13 347.0 14 487.0 15 741.0 16 1002.0 17 1404.0 18 1315.0 19 718.0 20 597.0 21 624.0 22 589.0 23 623.0 24 641.0 25 638.0 26 672.0 27 725.0 28 805.0 29 941.0 30 1152.0 31 1409.0 32 1752.0 33 2293.0 34 3144.0 35 4851.0 36 6869.0 37 11722.0 38 12524.0 39 1650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35336688706865 22.773775453139905 16.957794664298167 17.915062995493273 2 15.420652924351513 24.612880954729263 39.298747410987275 20.667718709931947 3 18.902258605385146 29.246802774764113 32.85498241115165 18.995956208699084 4 11.902883256073906 21.581681296643325 38.07245947989611 28.44297596738666 5 10.580401183821111 40.23840841828346 35.46366326866163 13.717527129233806 6 26.054508991682283 40.84722359207022 17.330768977874214 15.767498438373279 7 21.92523917546109 38.33711411381793 22.645231285136603 17.092415425584377 8 23.552618601439985 35.23358648124404 21.29401321629352 19.919781701022455 9 24.20521418943354 18.946641680639118 23.50001643817602 33.34812769175132 10-11 22.240852155044877 29.750632869776773 27.69092941447217 20.317585560706185 12-13 25.174865409115192 28.491349196564336 28.332716886532694 18.001068507787778 14-15 21.96880034191406 29.703784068119806 29.500772594272938 18.8266429956932 16-17 17.61679324062202 32.66676529572279 30.79445704704606 18.921984416609135 18-19 16.802281618831575 30.31774994246638 35.40043396784693 17.479534470855114 20-21 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33.885656047604954 17.484465923661112 46-47 18.73294539237926 30.937469178419963 32.743202814215735 17.58638261498504 48-49 18.665548870697307 30.884867015155997 32.59772495643883 17.85185915770786 50-51 18.379524607949502 31.185685636321793 32.656902390110794 17.77788736561791 52-53 18.330210079889536 31.858007035539337 31.566229411184537 18.245553473386593 54-55 18.0902127099977 31.22678107637177 31.80540487227537 18.877601341355163 56-57 18.76664365322024 31.43061445901963 31.150343557878816 18.65239832988132 58-59 18.51596146891541 30.538843409935236 31.843212677121347 19.10198244402801 60-61 18.280895551829566 31.007331426504912 32.200743005556106 18.511030016109412 62-63 18.18719794851563 30.80842949666305 32.433343196238944 18.571029358582372 64-65 18.076240260380708 31.28267087483973 31.803761054673373 18.837327810106192 66-67 17.82391425847388 30.68596508531413 31.594174310418516 19.895946345793472 68-69 17.54528717493507 30.48377552026827 32.29690633527304 19.67403096952362 70-71 18.2365124765756 30.349804385705365 31.816089686688365 19.597593451030672 72-73 18.18884176611763 29.584607291974883 32.085675773416185 20.140875168491306 74-75 17.597889338199032 30.327612848078378 31.534996876746558 20.539500936976033 76-77 17.755695827990927 29.31337738764507 32.296084426472035 20.63484235789197 78-79 17.630765690239013 29.61419600881086 31.975539994082258 20.77949830686787 80-81 17.908570864976824 29.312555478844065 31.507873886313575 21.270999769865536 82-83 17.58638261498504 29.522142223098925 31.567873228786535 21.3236019331295 84-85 17.518452155902224 29.525093288182404 31.34236351980011 21.614091036115266 86-87 17.36775487391919 29.757208140184765 30.521583325114243 22.3534536607818 88-89 17.9414472170168 29.415294078968994 30.078574481375547 22.564684222638657 90-91 17.833216883810778 28.611236693929555 30.945707122600798 22.60983929965887 92-93 17.508589088159386 28.907829631943187 30.97332040175563 22.610260878141798 94-95 17.39980931715817 28.904066804747348 30.25117532958543 23.444948548509057 96-97 17.601998882204033 29.437485616595982 30.20432652792846 22.756188973271527 98-99 17.163099582470327 29.911726994772657 29.442417069401976 23.48275635335503 100-101 16.591176252990163 30.184382937796446 29.17492128994073 24.04951951927266 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 620.0 1 398.0 2 171.5 3 178.5 4 150.5 5 135.5 6 192.0 7 294.5 8 408.0 9 411.0 10 324.0 11 260.0 12 240.5 13 230.0 14 223.5 15 214.0 16 222.0 17 243.0 18 247.5 19 271.0 20 292.0 21 302.5 22 349.0 23 406.0 24 439.5 25 519.5 26 672.5 27 837.5 28 970.0 29 1109.0 30 1273.0 31 1457.0 32 1675.0 33 1888.0 34 2048.0 35 2191.5 36 2294.0 37 2259.5 38 2173.0 39 2127.5 40 2076.5 41 2018.5 42 1900.5 43 1837.5 44 1848.5 45 1809.5 46 1757.0 47 1930.5 48 2693.0 49 2631.5 50 1829.0 51 1459.0 52 1125.5 53 954.0 54 849.5 55 702.5 56 591.5 57 522.5 58 458.0 59 376.0 60 290.5 61 221.5 62 161.0 63 99.0 64 69.5 65 51.0 66 35.5 67 26.0 68 22.5 69 19.0 70 12.0 71 7.5 72 7.0 73 6.5 74 5.5 75 5.5 76 4.0 77 2.0 78 2.5 79 3.0 80 2.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05917743367195976 2 0.0 3 0.0 4 0.0 5 0.02301344642798435 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002465726402998323 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001643817601998882 24-25 0.0 26-27 0.002465726402998323 28-29 0.0041095440049972055 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012328632014991616 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.001643817601998882 86-87 0.0 88-89 0.0 90-91 0.010684814412992735 92-93 0.001643817601998882 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.01561626721898938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 60834.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.95052109017983 #Duplication Level Percentage of deduplicated Percentage of total 1 84.09597084430047 54.62077127921886 2 9.53634338935007 12.387809448663576 3 2.9130390767361813 5.6761021797021405 4 1.252783964365256 3.254758851957787 5 0.6251265438347844 2.0301147384686193 6 0.3897550111358575 1.5188874642469672 7 0.2657420530471755 1.2082059374691783 8 0.17209961530674225 0.894236775487392 9 0.11642032800161976 0.6805404872275372 >10 0.5466693662684754 6.1560969194858135 >50 0.043024903826685564 1.885458789492718 >100 0.03290139704393602 3.9763947792352963 >500 0.007592630087062158 3.9977644080612813 >1k 0.0025308766956873858 1.7128579412828353 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1042 1.7128579412828353 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 955 1.5698458099089325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 889 1.4613538481770063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.9665647499753427 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 474 0.7791695433474701 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 343 0.5638294374856166 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 227 0.37314659565374625 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.28766808034980434 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 160 0.26301081631982115 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 154 0.2531479107078279 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.23999736989183681 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 146 0.23999736989183681 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 141 0.2317782818818424 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.22027155866785023 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 108 0.17753230101587927 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 106 0.17424466581188153 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 105 0.17260084820988264 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 97 0.15945030739389157 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 92 0.15123121938389716 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 91 0.14958740178189828 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 82 0.13479304336390835 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 0.11999868494591841 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 70 0.11506723213992175 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 65 0.10684814412992734 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 63 0.10356050892592956 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 63 0.10356050892592956 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 63 0.10356050892592956 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.1019166913239307 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0016438176019988823 0.0 22-23 0.0 0.0 0.0 0.008219088009994411 0.0 24-25 0.0 0.0 0.0 0.01479435841798994 0.0 26-27 0.0 0.0 0.0 0.023835355228983793 0.0 28-29 0.0 0.0 0.0 0.05178025446296479 0.0 30-31 0.0 0.0 0.0 0.11342341453792287 0.0 32-33 0.0 0.0 0.0 0.17753230101587927 0.0 34-35 0.0 0.0 0.0 0.2556136371108262 0.0 36-37 0.0 0.0 0.0 0.2934214419568005 0.0 38-39 0.0 0.0 0.0 0.3197225235887826 0.0 40-41 0.0 0.0 0.0 0.3468455140217641 0.0 42-43 0.0 0.0 0.0 0.3821875924647401 0.0 44-45 0.0 0.0 0.0 0.41506394450471773 0.0 46-47 0.0 0.0 0.0 0.46684419896768253 0.0 48-49 0.0 0.0 0.0 0.5136930006246507 0.0 50-51 0.0 0.0 0.0 0.5605418022816189 0.0 52-53 0.0 0.0 0.0 0.689581484038531 0.0 54-55 0.0 0.0 0.0 0.8399907946214288 0.0 56-57 0.0 0.0 0.0 1.0446460860702897 0.0 58-59 0.0 0.0 0.0 1.2986159055791169 0.0 60-61 0.0 0.0 0.0 1.6742282276358615 0.0 62-63 0.0 0.0 0.0 1.904362691915705 0.0 64-65 0.0 0.0 0.0 2.163263964230529 0.0 66-67 0.0 0.0 0.0 2.667094059243186 0.0 68-69 0.0 0.0 0.0 3.2580464871617845 0.0 70-71 0.0 0.0 0.0 3.6763980668705 0.0 72-73 0.0 0.0 0.0 4.110365913798205 0.0 74-75 0.0 0.0 0.0 4.983233060459611 0.0 76-77 0.0 0.0 0.0 5.532268139527238 0.0 78-79 0.0 0.0 0.0 5.903770917578985 0.0 80-81 0.0 0.0 0.0 6.281848966038728 0.0 82-83 0.0 0.0 0.0 7.02978597494822 0.0 84-85 0.0 0.0 0.0 7.814708879902685 0.0 86-87 0.0 0.0 0.0 8.733602919420061 0.0 88-89 0.0 0.0 0.0 10.231942663642041 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCCAC 50 0.0016047602 37.995396 9 GTATAGC 55 0.0025311585 34.626694 1 CATGGGG 70 2.0222568E-4 33.95238 4 ATTATAT 65 0.00578132 29.251284 3 TATATGT 70 0.00830179 27.161905 3 TAACAAA 70 0.008335227 27.139568 6 CCAGTTT 35 0.008367555 27.139568 20-21 GAGTACT 625 0.0 24.697006 12-13 GTACTTT 635 0.0 23.934107 14-15 TCTACTG 100 0.001629079 23.766666 2 AGTACTT 600 0.0 23.747122 12-13 TACTTTT 675 0.0 20.756742 14-15 ACTTTTT 735 0.0 20.677767 16-17 GAGCTAT 60 0.004742814 19.789267 64-65 GGTATCA 1085 0.0 18.869156 1 GTATCAA 1355 0.0 18.623028 1 TATCAAC 1460 0.0 17.25525 2 CGAGACA 195 2.910383E-10 17.049215 94-95 ATCAACG 1480 0.0 16.700901 3 TCAACGC 1455 0.0 16.66117 4 >>END_MODULE