##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937324_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66396 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.947346225676245 33.0 31.0 34.0 31.0 34.0 2 32.0455900957889 33.0 31.0 34.0 30.0 34.0 3 32.03546900415688 34.0 31.0 34.0 30.0 34.0 4 35.65449725887102 37.0 35.0 37.0 35.0 37.0 5 35.65772034459907 37.0 35.0 37.0 35.0 37.0 6 35.73865895535876 37.0 35.0 37.0 35.0 37.0 7 35.642598951744084 37.0 35.0 37.0 35.0 37.0 8 35.68529730706669 37.0 35.0 37.0 35.0 37.0 9 37.31580215675643 39.0 37.0 39.0 35.0 39.0 10-11 37.266100367492015 39.0 37.0 39.0 35.0 39.0 12-13 37.14920326525694 39.0 37.0 39.0 34.0 39.0 14-15 38.286116633532146 40.0 38.0 41.0 34.0 41.0 16-17 38.23109072835713 40.0 38.0 41.0 34.0 41.0 18-19 38.25978974637026 40.0 38.0 41.0 34.0 41.0 20-21 38.22280709681306 40.0 38.0 41.0 34.0 41.0 22-23 38.09661726610037 40.0 38.0 41.0 34.0 41.0 24-25 38.00495511777818 40.0 38.0 41.0 34.0 41.0 26-27 37.93803843605036 40.0 37.5 41.0 34.0 41.0 28-29 37.83148834267125 40.0 37.0 41.0 34.0 41.0 30-31 37.51566359419242 39.5 36.5 41.0 33.0 41.0 32-33 37.14569401771191 39.0 35.0 41.0 33.0 41.0 34-35 37.06136664859329 39.0 35.0 41.0 33.0 41.0 36-37 36.86252936923911 39.0 35.0 41.0 32.5 41.0 38-39 36.661583529128265 39.0 35.0 41.0 32.0 41.0 40-41 36.362085065365385 39.0 35.0 41.0 31.0 41.0 42-43 35.95825049701789 39.0 35.0 41.0 30.0 41.0 44-45 35.54287908910175 39.0 35.0 41.0 29.5 41.0 46-47 34.92147870353636 39.0 35.0 41.0 25.0 41.0 48-49 34.35632417615519 38.5 35.0 40.0 20.5 41.0 50-51 33.276695885294295 37.5 34.0 39.5 12.0 40.5 52-53 33.31550093379119 38.0 33.5 39.5 5.0 40.5 54-55 33.59197090186156 38.0 34.0 40.0 2.0 41.0 56-57 33.31116332309175 38.0 34.0 40.0 2.0 41.0 58-59 32.98231821194048 38.0 34.0 40.0 2.0 41.0 60-61 32.62237182962829 38.0 33.0 40.0 2.0 41.0 62-63 32.264910536779325 37.5 33.0 40.0 2.0 41.0 64-65 31.803489668052293 37.0 32.5 40.0 2.0 41.0 66-67 31.36856888969215 36.5 31.5 39.5 2.0 41.0 68-69 30.857378456533525 36.0 31.0 39.0 2.0 41.0 70-71 30.337437496234713 35.5 30.5 39.0 2.0 40.0 72-73 29.855352732092296 35.0 29.5 38.5 2.0 40.0 74-75 29.345660883185733 35.0 29.0 37.5 2.0 39.5 76-77 28.843906259413217 35.0 29.0 37.0 2.0 39.0 78-79 28.319785830471716 34.5 27.5 36.5 2.0 39.0 80-81 27.82370925959395 34.0 26.0 36.0 2.0 38.5 82-83 27.361173865895537 34.0 26.0 35.5 2.0 37.0 84-85 26.796147358274595 34.0 25.0 35.0 2.0 37.0 86-87 26.336059401168747 34.0 23.5 35.0 2.0 36.0 88-89 25.866121453099584 33.5 20.5 35.0 2.0 36.0 90-91 25.35471263329116 33.0 17.5 35.0 2.0 35.5 92-93 24.878140249412617 33.0 9.0 35.0 2.0 35.0 94-95 24.382869449966865 32.0 2.0 35.0 2.0 35.0 96-97 23.79309747575155 32.0 2.0 35.0 2.0 35.0 98-99 23.04293180312067 31.5 2.0 34.0 2.0 35.0 100-101 20.787479667449844 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43206031303033754 1101 2 0.5098982273069268 1101 3 0.42264594602913874 1101 4 0.42070286004104673 1101 5 0.4659147740647356 1101 6 0.4300564604386281 1101 7 0.42295313249872635 1101 8 0.4501842163681715 1101 9 0.43022559688619566 1101 10-11 0.2985911583515346 1101 12-13 0.3132316434502016 1101 14-15 0.12101303590212353 1101 16-17 0.1226544224940298 1101 18-19 -0.07101745286836092 1101 20-21 -0.06328079649876628 1101 22-23 0.27178250620368516 1101 24-25 0.6240028975980039 1101 26-27 0.7998312824705209 1101 28-29 0.3139716782674711 1101 30-31 0.5171654896783693 1101 32-33 0.5591068462218445 1101 34-35 0.22721532900879993 1101 36-37 0.9442678613426381 1101 38-39 0.666932160687729 1101 40-41 1.4578780127690223 1101 42-43 0.24138479337775465 1101 44-45 0.7442938763916729 1101 46-47 0.606048871539393 1101 48-49 1.128940017459179 1101 50-51 1.566446339928099 1101 52-53 0.7729523812636714 1101 54-55 0.33609671333598357 1101 56-57 0.2743385569464607 1101 58-59 -0.06931710918748735 1101 60-61 0.17785030962248527 1101 62-63 -0.15759876894397706 1101 64-65 0.43465740876021286 1101 66-67 -0.9721691664802243 1101 68-69 -0.4357359737955946 1101 70-71 -0.14763431803211446 1101 72-73 -0.5371472303783946 1101 74-75 -0.3528396847536506 1101 76-77 -0.5367764035846356 1101 78-79 -0.06472935862699813 1101 80-81 -0.2353008029679522 1101 82-83 -0.019001136639392513 1101 84-85 0.49280425523147997 1101 86-87 0.20344070789538193 1101 88-89 0.4538986640652567 1101 90-91 -0.1624537805898001 1101 92-93 -0.8534645335166395 1101 94-95 -0.2849114273111617 1101 96-97 -0.983890902596336 1101 98-99 -0.8749720865012485 1101 100-101 -1.1015587197863077 1102 1 -0.39352108231850025 1102 2 0.06803776219064872 1102 3 0.19008780649425816 1102 4 -0.09092504693570191 1102 5 0.09382175080892807 1102 6 0.3254052976479258 1102 7 -0.3212329140128958 1102 8 -0.28237392316670906 1102 9 0.08138838758387124 1102 10-11 -0.19559488816009463 1102 12-13 0.1271851318222943 1102 14-15 0.09775722194863334 1102 16-17 -0.03432232169200944 1102 18-19 0.4638662680618779 1102 20-21 -0.028397075568534547 1102 22-23 0.3066662271339098 1102 24-25 0.4670261534119504 1102 26-27 0.24750570107516978 1102 28-29 0.3081577247791003 1102 30-31 0.08693293153883275 1102 32-33 -0.022288502615360528 1102 34-35 0.47140137552042916 1102 36-37 0.29891902413333327 1102 38-39 1.0041414630133048 1102 40-41 0.7602035941643663 1102 42-43 1.1890592119824035 1102 44-45 1.1803403880195802 1102 46-47 0.9432581738649759 1102 48-49 1.1173121104824375 1102 50-51 1.6071440143467086 1102 52-53 1.9996965673101883 1102 54-55 2.2605153179871493 1102 56-57 1.9138734406673947 1102 58-59 2.2097526582543807 1102 60-61 2.1778503096224853 1102 62-63 2.801703556637417 1102 64-65 2.7893085715509116 1102 66-67 2.9289936242174477 1102 68-69 2.9886826308555747 1102 70-71 2.863993588944634 1102 72-73 2.8698295138076517 1102 74-75 2.8390207803626275 1102 76-77 3.1143863871130364 1102 78-79 2.9294566878846275 1102 80-81 1.7472573365669284 1102 82-83 1.3589058401047964 1102 84-85 1.3183856505803178 1102 86-87 1.1278593125465477 1102 88-89 1.2155265710420053 1102 90-91 0.24452296359624626 1102 92-93 0.8907215129949897 1102 94-95 0.8255536889679078 1102 96-97 0.8475044462408725 1102 98-99 1.026190704196427 1102 100-101 0.45658081509741066 1103 1 0.3098876465760547 1103 2 0.15437098933304583 1103 3 -0.28856781631555606 1103 4 0.38374841595057063 1103 5 0.4057045160207764 1103 6 0.6425445139588533 1103 7 0.08877027171504182 1103 8 0.32976370028024604 1103 9 0.5125766641050546 1103 10-11 0.09438599747227272 1103 12-13 -0.3137196149123298 1103 14-15 -0.25060212816286054 1103 16-17 0.13045945594417674 1103 18-19 0.10786117089717351 1103 20-21 0.9676207645079202 1103 22-23 0.7642164023489499 1103 24-25 1.1640634264288394 1103 26-27 0.7819115628145781 1103 28-29 0.2010376228358055 1103 30-31 0.011192249793062103 1103 32-33 0.3772814241129012 1103 34-35 0.43803087536115015 1103 36-37 1.0577594191635953 1103 38-39 -0.13762343026480295 1103 40-41 0.5657945466970205 1103 42-43 1.1139556758243927 1103 44-45 0.6930036565764439 1103 46-47 0.3358991423262694 1103 48-49 0.25692663740183264 1103 50-51 0.11996656930050875 1103 52-53 0.44259239400658856 1103 54-55 1.4012448496851349 1103 56-57 1.2040932558951738 1103 58-59 1.5296793865117806 1103 60-61 1.972211571170753 1103 62-63 1.2902349360576117 1103 64-65 2.102863843930649 1103 66-67 1.7592739682760694 1103 68-69 2.2471248098934815 1103 70-71 1.788969695985088 1103 72-73 2.0965736998541615 1103 74-75 2.150027470391297 1103 76-77 2.297884316389876 1103 78-79 2.2911164521407628 1103 80-81 2.182029557019302 1103 82-83 2.4876888920321605 1103 84-85 2.5562002412142775 1103 86-87 2.368859631119342 1103 88-89 2.0501076478180487 1103 90-91 1.4723821544213465 1103 92-93 0.8027954218831717 1103 94-95 1.2573791110768617 1103 96-97 1.9810660900764923 1103 98-99 1.4768915643429708 1103 100-101 1.3990784258014592 1104 1 -0.38855984200842286 1104 2 -0.3152180517628409 1104 3 -0.35797420900961896 1104 4 -0.36255295391244147 1104 5 -0.8205968538422397 1104 6 -0.6062226093288174 1104 7 -1.0235584954082455 1104 8 -0.7495057061124513 1104 9 -0.7193868062145796 1104 10-11 -0.5383080664546682 1104 12-13 -0.7790164185652984 1104 14-15 -0.7399171966560161 1104 16-17 -0.09501999611061507 1104 18-19 -0.5924128017055637 1104 20-21 -0.8480869980491548 1104 22-23 -1.4816283465095026 1104 24-25 -0.26351648224695623 1104 26-27 -0.22210670202560578 1104 28-29 -0.5908345232829149 1104 30-31 -1.0857027273758888 1104 32-33 -1.391668347576605 1104 34-35 -1.564800174867166 1104 36-37 -1.3945693479596812 1104 38-39 -0.6341531106301019 1104 40-41 -0.1845250880061684 1104 42-43 -0.15535939266876397 1104 44-45 -1.1738456584920485 1104 46-47 -1.4646488028792106 1104 48-49 -1.8112925406803555 1104 50-51 -0.5433211019323636 1104 52-53 -1.0109235877285698 1104 54-55 -1.362042821547739 1104 56-57 -0.5932583422783466 1104 58-59 -0.6467589696526019 1104 60-61 0.10079604605660109 1104 62-63 0.9337965798932295 1104 64-65 0.7439597343416082 1104 66-67 0.11434246142675164 1104 68-69 -0.5041080668188478 1104 70-71 -0.5418978839235855 1104 72-73 -0.7500929668125025 1104 74-75 -0.6325296072342717 1104 76-77 -0.5857686516466529 1104 78-79 -1.0121712190921137 1104 80-81 -0.8464635936656286 1104 82-83 0.20394459979472046 1104 84-85 0.4470678211229533 1104 86-87 -0.02903991225965541 1104 88-89 -0.4653261421362913 1104 90-91 -0.3826863387293358 1104 92-93 -0.423231975377103 1104 94-95 -0.413671117233644 1104 96-97 -0.6558288870924578 1104 98-99 -1.0421038694469829 1104 100-101 -0.566674998856076 1105 1 0.23239253894395873 1105 2 0.1590676625228724 1105 3 0.008692457657044628 1105 4 -0.20255295391244488 1105 5 0.4622602890149068 1105 6 0.18520596209975793 1105 7 0.6335843617346129 1105 8 0.657160960554215 1105 9 1.056803669975892 1105 10-11 0.8469300287834258 1105 12-13 0.609078819529941 1105 14-15 1.0429399462011233 1105 16-17 0.339265718175092 1105 18-19 0.5690157697230092 1105 20-21 0.17143681147465628 1105 22-23 0.023609748728596003 1105 24-25 0.34410256537208994 1105 26-27 0.37408377416487326 1105 28-29 0.3415464290980381 1105 30-31 0.6919163202431591 1105 32-33 -0.0440492999575568 1105 34-35 0.2318664917995008 1105 36-37 0.3668592234688859 1105 38-39 -0.3289150153920062 1105 40-41 0.10023681675573926 1105 42-43 0.200354893045521 1105 44-45 0.9504400557936705 1105 46-47 0.2082083399779293 1105 48-49 -0.3012925406803575 1105 50-51 -0.3176068162180812 1105 52-53 -0.40711406391904603 1105 54-55 0.27081432130940897 1105 56-57 0.09626546724546614 1105 58-59 1.0132410303473947 1105 60-61 1.1155579508184985 1105 62-63 0.8309394370360899 1105 64-65 0.5153883057701769 1105 66-67 0.38148531856960943 1105 68-69 0.3901776474668637 1105 70-71 0.017149735124032617 1105 72-73 0.5251451284255921 1105 74-75 0.41127991657524987 1105 76-77 0.7608980150200146 1105 78-79 0.8178287809078846 1105 80-81 0.9278221206200854 1105 82-83 0.7258493616994777 1105 84-85 1.0137344877896197 1105 86-87 1.2619124686927243 1105 88-89 1.8141976673875178 1105 90-91 2.1073136612706627 1105 92-93 2.0481965960514685 1105 94-95 2.2715669780044543 1105 96-97 2.133218731955157 1105 98-99 2.0788485115053987 1105 100-101 2.3976107154296393 1106 1 -0.4354646039131822 1106 2 -0.2587894803342685 1106 3 -0.8734503994858116 1106 4 -0.5489815253410129 1106 5 -0.3127397109850918 1106 6 -0.45407975218596164 1106 7 -0.4092727811225316 1106 8 -0.910696182302928 1106 9 -1.0074820443098176 1106 10-11 -2.0155699712165713 1106 12-13 -1.414135466184348 1106 14-15 -1.653488625227446 1106 16-17 -1.9410914246820496 1106 18-19 -1.6970556588484271 1106 20-21 -2.1035631885253494 1106 22-23 -1.981747394128547 1106 24-25 -1.9148260060564866 1106 26-27 -1.2884162258351353 1106 28-29 -0.8602392851876814 1106 30-31 -0.9884408226139882 1106 32-33 -0.9940492999575525 1106 34-35 -0.42706207962906717 1106 36-37 -1.208140776531117 1106 38-39 -0.8824864439634368 1106 40-41 -0.24083461181569987 1106 42-43 0.059283464474098935 1106 44-45 0.37365434150795096 1106 46-47 0.3867797685493599 1106 48-49 0.7165646021767813 1106 50-51 -0.16403538764664916 1106 52-53 0.1500287932238109 1106 54-55 0.37617146416654634 1106 56-57 0.41055118153118286 1106 58-59 -0.07783039822403026 1106 60-61 -0.563013477752925 1106 62-63 -0.9065605629639144 1106 64-65 -1.8738974085155355 1106 66-67 -1.8363718242875322 1106 68-69 -1.4151794953902765 1106 70-71 -1.1632074077331076 1106 72-73 -1.0194977287172655 1106 74-75 -1.0887200834247501 1106 76-77 -1.0623162706942715 1106 78-79 -0.8857426476635446 1106 80-81 -1.163249307951343 1106 82-83 -0.7598649240148028 1106 84-85 -0.7505512264960963 1106 86-87 -1.0970161027358465 1106 88-89 -0.4125880468981933 1106 90-91 0.07695651841351747 1106 92-93 -0.3875176896628183 1106 94-95 -0.26414730770983397 1106 96-97 0.42786158909801486 1106 98-99 0.4431342257911126 1106 100-101 0.2726107154296393 1107 1 -0.22060285276111102 1107 2 0.2544593675919984 1107 3 0.2538537479796297 1107 4 -0.37674650229953954 1107 5 -0.32253233771320566 1107 6 -0.04106131900623211 1107 7 0.16676408523691322 1107 8 0.5507093476509937 1107 9 0.5074949142155276 1107 10-11 0.5054553744055497 1107 12-13 0.26184379648846345 1107 14-15 -0.17249784181731087 1107 16-17 0.23143161679260515 1107 18-19 -0.05563860815718158 1107 20-21 0.2092248299078321 1107 22-23 -0.04367135726219118 1107 24-25 -0.28953798762330507 1107 26-27 0.19574276033998217 1107 28-29 0.36067085306117264 1107 30-31 0.4502112511187377 1107 32-33 0.5336004696277001 1107 34-35 0.8560600401865983 1107 36-37 -0.09005321892743012 1107 38-39 0.5005780721655952 1107 40-41 -0.34538530305994186 1107 42-43 -1.1063271346042498 1107 44-45 -0.6848134004275366 1107 46-47 -0.24916493191146571 1107 48-49 -0.18032479874486995 1107 50-51 0.21442083355150743 1107 52-53 -0.29812788880384034 1107 54-55 -0.0930105634832259 1107 56-57 -0.6723981272245823 1107 58-59 0.03420877228288788 1107 60-61 0.1489680890673526 1107 62-63 0.7221836766674237 1107 64-65 0.46654037950289506 1107 66-67 0.8888585904590087 1107 68-69 0.7775048364069583 1107 70-71 0.8330483526355543 1107 72-73 0.7820575708219053 1107 74-75 0.7638144787872356 1107 76-77 0.47541414405226945 1107 78-79 0.15169974864982194 1107 80-81 0.8474073740763082 1107 82-83 0.8956650299022435 1107 84-85 0.9314764232734909 1107 86-87 1.0847235286005628 1107 88-89 1.8745663309819882 1107 90-91 2.0524749515932434 1107 92-93 1.5041873794616087 1107 94-95 1.455898775239472 1107 96-97 1.0007302526924846 1107 98-99 1.8290789262519382 1107 100-101 2.643002420498764 1108 1 -0.08528300585022919 1108 2 -1.006065509389959 1108 3 -1.1116465253938017 1108 4 -1.2466207505226095 1108 5 -0.970427362316812 1108 6 -0.07605311780339008 1108 7 -0.2676262920184129 1108 8 -0.591991581818661 1108 9 -0.37249415084735205 1108 10-11 -0.394353264194784 1108 12-13 -0.4699768705427019 1108 14-15 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2.0344524377593416 2312 28-29 1.8203482724160054 2312 30-31 2.1760177027316345 2312 32-33 1.6384391793051165 2312 34-35 0.4367052014769186 2312 36-37 0.6357532326854738 2312 38-39 1.186061943133339 2312 40-41 -0.07119175467283867 2312 42-43 -0.5095529410558584 2312 44-45 0.6377672447337588 2312 46-47 0.37986732615304675 2312 48-49 -0.7932280245513255 2312 50-51 -0.9871920696743004 2312 52-53 -1.3545795017070645 2312 54-55 -1.2865589505799981 2312 56-57 -1.9304626433536143 2312 58-59 -1.8286944535235676 2312 60-61 -2.480064168997167 2312 62-63 -2.8745905168809607 2312 64-65 -2.517330588239041 2312 66-67 -2.1837220547022795 2312 68-69 -2.1337854861736893 2312 70-71 -2.2234032602676734 2312 72-73 -3.3872972678877744 2312 74-75 -4.252314553470828 2312 76-77 -4.129424565625147 2312 78-79 -3.8966873481243702 2312 80-81 -4.870334561407564 2312 82-83 -4.160786583000981 2312 84-85 -3.512071963823285 2312 86-87 -2.931405503657505 2312 88-89 -3.294788507727688 2312 90-91 -3.6813960161486925 2312 92-93 -2.552264233441619 2312 94-95 -2.358617353792784 2312 96-97 -2.4186245860171915 2312 98-99 -3.461243654393222 2312 100-101 -4.195707256920592 2313 1 0.5514401579915749 2313 2 -0.2552180517628422 2313 3 0.2420257909903789 2313 4 -0.26921962057910775 2313 5 -0.38059685384223485 2313 6 0.7804440573378528 2313 7 0.7764415045917517 2313 8 0.15716096055421502 2313 9 -0.5527201395479153 2313 10-11 0.6516919335453295 2313 12-13 0.5043169147680331 2313 14-15 1.0167494700106516 2313 16-17 0.24164667055604383 2313 18-19 0.8309205316277684 2313 20-21 0.4452463352841747 2313 22-23 0.5117049868238368 2313 24-25 0.31315018441971176 2313 26-27 0.8312266313077288 2313 28-29 1.0891654767170778 2313 30-31 1.0776306059574452 2313 32-33 1.108331652423395 2313 34-35 1.3818664917994994 2313 36-37 1.4120973187069765 2313 38-39 0.4591802227032318 2313 40-41 0.7788082453271556 2313 42-43 -0.06869272600209797 2313 44-45 -0.23051232515872044 2313 46-47 0.03201786378745908 2313 48-49 0.032040792652971106 2313 50-51 -0.13665443526569732 2313 52-53 0.019076412271424203 2313 54-55 0.12795717845226307 2313 56-57 -0.6299250089450155 2313 58-59 -1.936758969652601 2313 60-61 -1.4892039539434023 2313 62-63 -1.9928700867734328 2313 64-65 -1.4560402656583946 2313 66-67 -2.4423242052399132 2313 68-69 -3.7907747334855166 2313 70-71 -2.8185645505902563 2313 72-73 -3.5200929668125056 2313 74-75 -3.5625296072342714 2313 76-77 -3.1391019849799875 2313 78-79 -4.482171219092113 2313 80-81 -4.169796926998959 2313 82-83 -3.069388733538613 2313 84-85 -3.1862655122103796 2313 86-87 -2.9523732455929874 2313 88-89 -4.195326142136292 2313 90-91 -3.242686338729335 2313 92-93 -3.2398986420437694 2313 94-95 -2.423671117233642 2313 96-97 -2.7858288870924603 2313 98-99 -2.5687705361136537 2313 100-101 -3.9166749988560774 2314 1 -0.5877755282829327 2314 2 -0.866982757645193 2314 3 -1.773660483519425 2314 4 -1.3378470715595014 2314 5 -1.4041262656069406 2314 6 -1.0685755505052867 2314 7 -1.800029083643544 2314 8 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72-73 -3.712249829557603 2314 74-75 -3.519392352332307 2314 76-77 -4.030278455568222 2314 78-79 -3.170406513209759 2314 80-81 -3.812934181900925 2314 82-83 -3.9762514786366516 2314 84-85 -5.139206688680968 2314 86-87 -5.067079127945931 2314 88-89 -4.219835946057859 2314 90-91 -3.6662157504940396 2314 92-93 -4.1144084459653385 2314 94-95 -3.245239744684625 2314 96-97 -3.6083779067003015 2314 98-99 -4.6619077910156115 2314 100-101 -4.7284397047384275 2315 1 -0.05430696844520355 2315 2 0.3896095344440553 2315 3 0.6604165955880781 2315 4 0.2250332529841117 2315 5 0.7297479737439687 2315 6 -0.23794674725984777 2315 7 0.16954495286761784 2315 8 0.5295747536576627 2315 9 0.43463618229116463 2315 10-11 0.3338758415913077 2315 12-13 0.40144335154963784 2315 14-15 0.2104276309301909 2315 16-17 0.7180834521652386 2315 18-19 0.12344926725995009 2315 20-21 0.2923727720657894 2315 22-23 0.16572797532958106 2315 24-25 0.6470582303967234 2315 26-27 0.6139852519973772 2315 28-29 0.3937631778665107 2315 30-31 -0.4413349112839384 2315 32-33 -0.42385225562257745 2315 34-35 0.2197975262822638 2315 36-37 0.31094789341963036 2315 38-39 0.3373411422434529 2315 40-41 0.5322565211892254 2315 42-43 0.9715371590553801 2315 44-45 1.1171888242665702 2315 46-47 0.09121326608630653 2315 48-49 -0.6030166786113895 2315 50-51 -1.2619417915875388 2315 52-53 -1.529774162441214 2315 54-55 -0.8427324767201512 2315 56-57 -0.5327985721634008 2315 58-59 0.5684134441405035 2315 60-61 0.690681103527865 2315 62-63 -1.8262034201067685 2315 64-65 -0.1491437139342544 2315 66-67 1.3502045303922756 2315 68-69 1.3689953814570153 2315 70-71 0.5969526907890561 2315 72-73 1.8379530101989907 2315 74-75 0.9679301628806734 2315 76-77 -0.44255026084205795 2315 78-79 -0.1597574259886656 2315 80-81 0.6307777856447174 2315 82-83 -0.5578944806650519 2315 84-85 -2.010403443244865 2315 86-87 -2.302373245592989 2315 88-89 -2.157969820297211 2315 90-91 -0.6357897870051978 2315 92-93 -0.14736990641158698 2315 94-95 -0.3725216919462859 2315 96-97 -0.8680127951384371 2315 98-99 -0.4768165131251436 2315 100-101 -0.6011577574767664 2316 1 0.7181068246582463 2316 2 0.6047819482371608 2316 3 1.308692457657049 2316 4 0.9174470460875597 2316 5 0.9394031461577654 2316 6 0.9537773906711848 2316 7 1.2564415045917556 2316 8 1.1171609605542159 2316 9 1.2939465271187487 2316 10-11 -0.2916413997879985 2316 12-13 1.2276502481013623 2316 14-15 1.6400828033439865 2316 16-17 1.404980003889385 2316 18-19 1.2875871982944318 2316 20-21 1.438579668617514 2316 22-23 1.5650383201571643 2316 24-25 1.2698168510863752 2316 26-27 1.7712266313077265 2316 28-29 2.1158321433837486 2316 30-31 1.8276306059574452 2316 32-33 1.241664985756735 2316 34-35 1.3418664917995073 2316 36-37 -0.1945693479596855 2316 38-39 1.6125135560365678 2316 40-41 1.588808245327158 2316 42-43 1.584640607331238 2316 44-45 1.7861543415079453 2316 46-47 1.8153511971207905 2316 48-49 0.32870745931964507 2316 50-51 -1.643321101932365 2316 52-53 -1.3442569210619055 2316 54-55 -2.402042821547738 2316 56-57 -1.9465916756116783 2316 58-59 -1.3667589696526008 2316 60-61 -1.465870620610069 2316 62-63 -1.186203420106768 2316 64-65 -1.4560402656583946 2316 66-67 -1.8656575385732452 2316 68-69 -1.1241080668188523 2316 70-71 -1.6885645505902538 2316 72-73 -3.383426300145839 2316 74-75 -3.2658629405676045 2316 76-77 -3.1391019849799875 2316 78-79 -2.7721712190921153 2316 80-81 -3.5664635936656275 2316 82-83 -2.8427220668719464 2316 84-85 -2.8062655122103806 2316 86-87 -2.362373245592991 2316 88-89 -2.1186594754696237 2316 90-91 -2.402686338729339 2316 92-93 -2.0632319753771036 2316 94-95 -1.347004450566974 2316 96-97 -0.5024955537591289 2316 98-99 0.4445627972196817 2316 100-101 -0.10667499885607512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 361.0 3 68.0 4 44.0 5 41.0 6 36.0 7 51.0 8 71.0 9 94.0 10 109.0 11 156.0 12 274.0 13 418.0 14 512.0 15 789.0 16 1009.0 17 1423.0 18 1204.0 19 723.0 20 738.0 21 777.0 22 793.0 23 773.0 24 773.0 25 829.0 26 891.0 27 850.0 28 960.0 29 1179.0 30 1401.0 31 1675.0 32 2085.0 33 2786.0 34 3723.0 35 5717.0 36 8073.0 37 11940.0 38 11455.0 39 1595.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.138877564886535 21.98668297323006 16.350349534206035 19.52408992767737 2 14.65056603773585 23.603018867924526 40.22339622641509 21.523018867924527 3 18.58934689749898 28.440976257678898 32.47551054291881 20.49416630190331 4 11.277151353227973 19.94747090522121 39.51455871032016 29.26081903123066 5 10.67153703340327 39.623552852032425 35.523992090685425 14.180918023878885 6 25.88666938442723 39.70799172592895 18.133502438435173 16.27183645120865 7 21.99338658291685 37.646650258950004 23.168098567092965 17.19186459104018 8 23.302583304395093 34.60359639454653 21.70217263297752 20.391647668080868 9 24.339175460048608 18.1830533037453 23.40926588469725 34.06850535150884 10-11 22.029983241994657 28.96417410208796 28.073433277473313 20.93240937844407 12-13 24.396687726926185 28.204895944201635 28.577036013798622 18.82138031507356 14-15 21.713962264150943 29.19622641509434 29.446037735849057 19.64377358490566 16-17 17.605761391430384 32.72639429900051 30.071413473442643 19.596430836126462 18-19 16.749947163431056 30.69729174843755 34.71211618006703 17.84064490806437 20-21 18.497245075100007 29.260321533700655 34.36485772511133 17.877575666088006 22-23 17.76940899451333 29.84762609148472 34.154698384187526 18.22826652981442 24-25 17.92749007592109 30.023546103572667 34.332030247686895 17.716933572819343 26-27 17.77740878832249 29.933430947816504 34.848370492248705 17.4407897716123 28-29 16.953771363005014 30.987680415484025 34.611087626064375 17.447460595446586 30-31 17.096591552485563 30.85569773147624 34.42796210319707 17.61974861284113 32-33 17.11809644593411 31.04447866654588 34.13503638614609 17.702388501373918 34-35 17.353032213255222 30.9864790391134 33.93300669631061 17.727482051320766 36-37 17.62015457070402 30.662812462263012 34.06065088757396 17.656382079459 38-39 17.52963994355015 31.08364086425623 33.74614171326798 17.640577478925643 40-41 17.150144892538037 31.381308862593578 34.0248430330838 17.44370321178459 42-43 17.19044815770804 31.586891877613247 32.99219610862051 18.230463856058204 44-45 17.496697240780584 31.367531045936666 32.67806590420111 18.457705809081645 46-47 18.26884602317768 31.375938998150314 32.09240874259182 18.262806236080177 48-49 18.057180820945664 31.282604920842232 31.943951622035833 18.716262636176268 50-51 17.637732259209205 31.608392664346276 32.10745267989943 18.646422396545084 52-53 17.970248433134486 31.89760628256437 31.396964434040626 18.735180850260516 54-55 17.81817907269968 30.952291070525256 31.753354121208915 19.47617573556614 56-57 18.232110915718845 30.980324082809425 31.329011222895613 19.458553778576118 58-59 18.226816569833247 30.42556402324002 31.60642873311703 19.7411906738097 60-61 17.839412093135554 30.39279301030648 32.43371712263653 19.33407777392144 62-63 17.86126600617233 30.05878047492209 32.618258920823685 19.4616945980819 64-65 17.51346783563959 30.498423093754244 32.525766195355295 19.462342875250872 66-67 17.406001705287146 29.82517034007651 32.414037682318586 20.354790272317757 68-69 17.372507299848344 29.833178659544128 32.61051630865343 20.183797731954094 70-71 17.581148988184164 29.55015316824362 32.74103248977621 20.127665353796008 72-73 17.654116049196407 28.817626197842 33.13363012148193 20.394627631479665 74-75 17.4570835691379 29.163554046406336 32.510092435389545 20.86926994906621 76-77 17.539794555184045 28.18337572551003 32.96777942985667 21.309050289449257 78-79 17.43964014279569 28.079881053910654 33.09282474320173 21.387654060091926 80-81 17.688917731930758 28.196169576963133 32.37975218460888 21.73516050649723 82-83 17.32349412590262 28.057586526925775 32.92286333008881 21.696056017082796 84-85 17.648434729463606 27.894184844529285 32.42664091207473 22.030739513932378 86-87 17.68736745983231 27.813624940569177 32.10622835020037 22.392779249398146 88-89 18.039727702223363 27.39279407103289 31.53084481743672 23.03663340930703 90-91 17.686884849948676 26.532214238270633 32.57879958939677 23.202101322383914 92-93 17.93737878364815 26.24687761770721 32.90519277644875 22.910550822195894 94-95 17.60955032863212 26.364521313924794 32.36894332133505 23.65698503610803 96-97 17.17157994837658 26.04416670440309 32.729550634726564 24.05470271249377 98-99 17.19916370415657 26.261802866653085 32.45175071137981 24.087282717810535 100-101 17.33189473048832 25.725086014365907 32.870314480593954 24.07270477455182 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 367.0 1 256.5 2 159.0 3 181.0 4 152.5 5 133.5 6 205.5 7 345.5 8 485.0 9 493.0 10 393.0 11 297.0 12 253.0 13 262.0 14 263.5 15 233.0 16 209.0 17 230.5 18 264.0 19 264.0 20 264.0 21 300.5 22 352.5 23 419.5 24 489.0 25 534.0 26 662.0 27 828.5 28 974.0 29 1157.5 30 1375.0 31 1587.5 32 1810.0 33 2036.0 34 2239.0 35 2397.0 36 2508.5 37 2536.5 38 2459.5 39 2393.5 40 2316.0 41 2239.0 42 2164.0 43 2094.5 44 2031.0 45 1991.0 46 1959.0 47 2122.0 48 2904.5 49 2887.5 50 2088.0 51 1609.0 52 1220.0 53 1037.0 54 945.0 55 825.0 56 683.0 57 572.0 58 480.0 59 421.5 60 345.0 61 258.5 62 186.0 63 128.0 64 77.0 65 52.5 66 43.5 67 29.0 68 20.0 69 17.0 70 14.0 71 13.5 72 10.0 73 6.5 74 3.0 75 1.0 76 1.5 77 2.5 78 3.0 79 2.0 80 2.0 81 2.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2485089463220676 2 0.219892764624375 3 0.21537442014579194 4 0.2213988794505693 5 0.2183866497981806 6 0.2485089463220676 7 0.25152117597445633 8 0.2454967166696789 9 0.23043556840773538 10-11 0.23947225736490152 12-13 0.23721308512560998 14-15 0.219892764624375 16-17 0.24399060184348456 18-19 0.23194168323392977 20-21 0.22742333875534673 22-23 0.21763359238508345 24-25 0.21537442014579194 26-27 0.22441110910295803 28-29 0.24097837219109586 30-31 0.24624977408277607 32-33 0.24399060184348456 34-35 0.2492620037351648 36-37 0.22290499427676363 38-39 0.21462136273269475 40-41 0.21085607566720885 42-43 0.2213988794505693 44-45 0.24624977408277607 46-47 0.25378034821374784 48-49 0.25227423338755345 50-51 0.25829869269233086 52-53 0.2726067835411772 54-55 0.25829869269233086 56-57 0.2221519368636665 58-59 0.19730104223145972 60-61 0.19127658292668231 62-63 0.19956021447075123 64-65 0.19127658292668231 66-67 0.19805409964455692 68-69 0.1920296403397795 70-71 0.19428881257907105 72-73 0.19730104223145972 74-75 0.20106632929694562 76-77 0.2251641665160552 78-79 0.2221519368636665 80-81 0.2063377311886258 82-83 0.19654798481836253 84-85 0.19504186999216822 86-87 0.21462136273269475 88-89 0.2183866497981806 90-91 0.22892945358154107 92-93 0.21160913308030604 94-95 0.20558467377552866 96-97 0.2213988794505693 98-99 0.22817639616844387 100-101 0.19278269775287668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 66396.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.67269112597144 #Duplication Level Percentage of deduplicated Percentage of total 1 84.05168519020512 56.03952045303934 2 9.731634589319599 12.976685342490512 3 2.9185867895545314 5.837701066329297 4 1.2898707870244872 3.439966263027893 5 0.6483238456672992 2.161274775588891 6 0.35917592843589047 1.4368335441894091 7 0.20104816119996385 0.9383095367190795 8 0.13553808620222282 0.7229351165732876 9 0.09487666034155598 0.569311404301464 >10 0.49019607843137253 5.751852521236219 >50 0.04066142586066685 1.9474064702692935 >100 0.029366585343814946 3.430929574070727 >500 0.009035872413481522 4.747273932164588 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 954 1.4368335441894091 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 872 1.3133321284414725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 788 1.186818483041147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 538 0.8102897764925598 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 375 0.564793059822881 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 286 0.4307488402915839 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 219 0.32983914693656247 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 206 0.3102596541960359 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 186 0.2801373576721489 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 153 0.23043556840773538 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 143 0.21537442014579194 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 140 0.21085607566720885 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.19579492740526538 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.1807337791433219 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 115 0.17320320501235015 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 104 0.1566359419242123 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 101 0.15211759744562925 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 94 0.1415747936622688 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 92 0.13856256400988012 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 90 0.1355503343574914 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 86 0.129525875052714 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 83 0.12500753057413097 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 83 0.12500753057413097 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 75 0.11295861196457617 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 74 0.11145249713838182 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 74 0.11145249713838182 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 73 0.1099463823121875 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 68 0.10241580818121573 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.530574130971745E-4 0.0 16-17 0.0 0.0 0.0 0.001506114826194349 0.0 18-19 0.0 0.0 0.0 0.001506114826194349 0.0 20-21 0.0 0.0 0.0 0.003012229652388698 0.0 22-23 0.0 0.0 0.0 0.0067775167178745705 0.0 24-25 0.0 0.0 0.0 0.012801976022651967 0.0 26-27 0.0 0.0 0.0 0.017320320501235012 0.0 28-29 0.0 0.0 0.0 0.05120790409060787 0.0 30-31 0.0 0.0 0.0 0.10919332489909031 0.0 32-33 0.0 0.0 0.0 0.1807337791433219 0.0 34-35 0.0 0.0 0.0 0.23269474064702694 0.0 36-37 0.0 0.0 0.0 0.276372070606663 0.0 38-39 0.0 0.0 0.0 0.3125188264353274 0.0 40-41 0.0 0.0 0.0 0.3343574914151455 0.0 42-43 0.0 0.0 0.0 0.36673896017832397 0.0 44-45 0.0 0.0 0.0 0.39686125670221095 0.0 46-47 0.0 0.0 0.0 0.44430387372733293 0.0 48-49 0.0 0.0 0.0 0.5075606964274957 0.0 50-51 0.0 0.0 0.0 0.5617808301704922 0.0 52-53 0.0 0.0 0.0 0.7086270257244413 0.0 54-55 0.0 0.0 0.0 0.880324115910597 0.0 56-57 0.0 0.0 0.0 1.1107596843183325 0.0 58-59 0.0 0.0 0.0 1.3532441713356227 0.0 60-61 0.0 0.0 0.0 1.7199831315139464 0.0 62-63 0.0 0.0 0.0 1.9895776854027352 0.0 64-65 0.0 0.0 0.0 2.2810109042713416 0.0 66-67 0.0 0.0 0.0 2.7441412133261043 0.0 68-69 0.0 0.0 0.0 3.3029098138442077 0.0 70-71 0.0 0.0 0.0 3.8021868787276345 0.0 72-73 0.0 0.0 0.0 4.279625278631243 0.0 74-75 0.0 0.0 0.0 5.140369901801313 0.0 76-77 0.0 0.0 0.0 5.731519971082595 0.0 78-79 0.0 0.0 0.0 6.15925658172179 0.0 80-81 0.0 0.0 0.0 6.591511536839569 0.0 82-83 0.0 0.0 0.0 7.303903849629496 0.0 84-85 0.0 0.0 0.0 8.111181396469668 0.0 86-87 0.0 0.0 0.0 9.0351828423399 0.0 88-89 0.0 0.0 0.0 10.38692089884933 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCATA 20 1.72851E-5 95.03477 1 GTCTTAC 15 6.5805693E-4 95.03477 1 TACTGAG 20 0.0020624246 71.27607 7 TAGCCCT 30 1.2898812E-4 63.35651 4 CCCATAC 35 2.7637315E-4 54.305584 2 GCCCTGG 40 5.341569E-4 47.517384 6 GTGTATG 45 9.5777353E-4 42.205772 9 AGCCCTG 50 0.0016019462 38.01391 5 CCCTGGC 55 0.0025575687 34.558098 7 ATGGGCA 65 0.0057944916 29.241468 5 AATTATG 65 0.0057944916 29.241468 2 GTATAGC 65 0.0057944916 29.241468 1 CCTGGCT 65 0.0057944916 29.241468 8 GAGTACT 635 0.0 26.918642 12-13 GTACTTT 645 0.0 26.501303 14-15 GCTGGGA 50 0.0016496934 23.740747 84-85 ACTTTTT 735 0.0 23.579248 16-17 TGGGGAT 55 0.0028642782 21.59065 38-39 GGTATCA 1290 0.0 20.627703 1 AGTACTT 645 0.0 19.875977 12-13 >>END_MODULE