##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937324_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66396 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.291960359057775 33.0 31.0 34.0 31.0 34.0 2 32.48963792999578 34.0 31.0 34.0 31.0 34.0 3 32.486625700343396 34.0 31.0 34.0 31.0 34.0 4 36.153638773420084 37.0 35.0 37.0 35.0 37.0 5 36.10640701247063 37.0 35.0 37.0 35.0 37.0 6 36.18409241520573 37.0 36.0 37.0 35.0 37.0 7 36.136966082294116 37.0 35.0 37.0 35.0 37.0 8 36.135911801915775 37.0 35.0 37.0 35.0 37.0 9 37.82385987107657 39.0 38.0 39.0 35.0 39.0 10-11 37.77509940357853 39.0 37.0 39.0 35.0 39.0 12-13 37.696954635821434 39.0 37.0 39.0 35.0 39.0 14-15 38.81692421230194 40.0 38.0 41.0 35.0 41.0 16-17 38.79135038255317 40.0 38.0 41.0 35.5 41.0 18-19 38.850774143020665 40.0 38.0 41.0 35.0 41.0 20-21 38.841699801192846 40.0 38.0 41.0 35.0 41.0 22-23 38.707309175251524 40.0 38.0 41.0 35.0 41.0 24-25 38.63409693355021 40.0 38.0 41.0 35.0 41.0 26-27 38.47274685222001 40.0 38.0 41.0 34.5 41.0 28-29 38.30436321465149 40.0 38.0 41.0 34.0 41.0 30-31 38.18782004940057 40.0 37.0 41.0 34.0 41.0 32-33 37.93184077354057 40.0 36.0 41.0 34.0 41.0 34-35 37.72487047412495 40.0 35.0 41.0 33.0 41.0 36-37 37.46442556780529 40.0 35.0 41.0 33.0 41.0 38-39 37.236557925176214 40.0 35.0 41.0 33.0 41.0 40-41 36.96901921802518 40.0 35.0 41.0 33.0 41.0 42-43 36.59620308452317 40.0 35.0 41.0 32.5 41.0 44-45 36.09739291523586 39.0 35.0 41.0 30.5 41.0 46-47 35.421599192722454 39.0 35.0 41.0 27.5 41.0 48-49 34.81156997409482 39.0 35.0 41.0 23.0 41.0 50-51 34.40227573950238 39.0 35.0 41.0 18.0 41.0 52-53 34.1118214952708 39.0 35.0 40.5 9.5 41.0 54-55 33.80949153563468 38.5 35.0 40.0 2.0 41.0 56-57 33.47126332911621 38.0 34.0 40.0 2.0 41.0 58-59 33.16183203807458 38.0 34.0 40.0 2.0 41.0 60-61 32.77634947888427 38.0 33.0 40.0 2.0 41.0 62-63 32.347129345141276 37.5 33.0 40.0 2.0 41.0 64-65 31.928128200494008 37.0 32.5 40.0 2.0 41.0 66-67 31.476828423399 36.0 32.0 39.0 2.0 41.0 68-69 31.027192903186936 36.0 31.0 39.0 2.0 40.5 70-71 30.578144767757095 35.0 31.0 39.0 2.0 40.0 72-73 30.06867883607446 35.0 30.5 38.0 2.0 40.0 74-75 29.568309837942046 35.0 29.5 37.0 2.0 39.5 76-77 28.303512259774685 33.5 28.0 36.0 2.0 38.5 78-79 28.7706187119706 34.0 29.0 36.0 2.0 39.0 80-81 28.52464003855654 34.0 29.0 36.0 2.0 38.5 82-83 28.12699560214471 34.0 29.0 35.5 2.0 37.0 84-85 27.69785077414302 34.0 27.5 35.0 2.0 37.0 86-87 27.28202000120489 34.0 26.5 35.0 2.0 36.0 88-89 26.791177179348153 34.0 25.5 35.0 2.0 36.0 90-91 26.293270678956564 34.0 24.5 35.0 2.0 35.5 92-93 25.98511205494307 33.5 23.5 35.0 2.0 35.0 94-95 25.589312609193325 33.0 20.0 35.0 2.0 35.0 96-97 25.174867461895296 33.0 14.0 35.0 2.0 35.0 98-99 24.55851256099765 33.0 2.0 35.0 2.0 35.0 100-101 22.577768239050545 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.19964201411859506 1101 2 -0.017837603136520386 1101 3 -0.16084893402757672 1101 4 0.12230517469290447 1101 5 0.08928698545473424 1101 6 -0.06426268336205254 1101 7 0.019684115251621392 1101 8 -0.014588072342171188 1101 9 0.36909647243015087 1101 10-11 0.2721263341294744 1101 12-13 -0.23273324505358062 1101 14-15 0.2686106835837947 1101 16-17 -0.185746414899711 1101 18-19 0.07182939752120632 1101 20-21 0.2655240966682797 1101 22-23 -0.6138373340668934 1101 24-25 -0.29057556916737326 1101 26-27 -0.04997050536216108 1101 28-29 -0.0926596453062487 1101 30-31 0.3143561157409991 1101 32-33 0.49506291765087695 1101 34-35 0.42180198293524285 1101 36-37 0.6648062277128517 1101 38-39 0.7442476159798375 1101 40-41 1.0638299180416126 1101 42-43 0.7266673908023549 1101 44-45 0.9466845213098622 1101 46-47 0.7266847736967463 1101 48-49 1.3245711888932945 1101 50-51 1.3795757424891377 1101 52-53 1.64825929487494 1101 54-55 1.6091493998280626 1101 56-57 1.219340124737876 1101 58-59 1.7623958649705926 1101 60-61 1.514480175049826 1101 62-63 1.0623247721519 1101 64-65 1.7103829359295588 1101 66-67 1.2721541387728088 1101 68-69 1.6429670215632832 1101 70-71 1.5598457450786114 1101 72-73 1.2565646485526756 1101 74-75 1.3638952908735007 1101 76-77 1.3663061534963994 1101 78-79 1.2683240388352033 1101 80-81 1.461271557745544 1101 82-83 1.0197753808478716 1101 84-85 1.6188189193662517 1101 86-87 1.0442035795155498 1101 88-89 0.5237545970592876 1101 90-91 1.3620289121296274 1101 92-93 1.0753748647115309 1101 94-95 0.8257155161523535 1101 96-97 0.4748652611697004 1101 98-99 0.47220527809594515 1101 100-101 1.7464050584467579 1102 1 -0.2112699210953366 1102 2 -0.23876783569466653 1102 3 -0.06782567821362306 1102 4 0.04090982585569236 1102 5 0.1590544273151977 1102 6 -0.21542547405972812 1102 7 -0.2942693731204713 1102 8 -0.026215979318919835 1102 9 0.09002670498828991 1102 10-11 -0.3906643635449356 1102 12-13 -0.22110533807683197 1102 14-15 -0.027900944323178578 1102 16-17 -0.028769670713657547 1102 18-19 -0.17817060247878658 1102 20-21 -0.2344759033317203 1102 22-23 -0.34639547360178113 1102 24-25 -0.49987789474877786 1102 26-27 -0.8115984123389026 1102 28-29 -0.6217294127481097 1102 30-31 -0.09262062844504726 1102 32-33 0.2334350106741283 1102 34-35 0.2008717503771109 1102 36-37 0.5601550649221494 1102 38-39 -0.2034268026248114 1102 40-41 0.30801596455324187 1102 42-43 0.16852785591863295 1102 44-45 0.5571496375889353 1102 46-47 0.7790103550920975 1102 48-49 -0.04752183436252011 1102 50-51 -0.2599591412317892 1102 52-53 0.7703523181307617 1102 54-55 0.5568238184327114 1102 56-57 0.603061054970432 1102 58-59 0.2972795859008244 1102 60-61 1.3807592448172699 1102 62-63 0.45185965587283405 1102 64-65 0.4371271219760722 1102 66-67 1.2256425108658284 1102 68-69 1.7301763238888732 1102 70-71 1.7982178381018663 1102 72-73 0.9774948811108182 1102 74-75 0.5034301745944276 1102 76-77 0.84886429303128 1102 78-79 0.5939054341840411 1102 80-81 -0.13756565155678047 1102 82-83 0.3918684041036862 1102 84-85 -0.10792526668025815 1102 86-87 0.05583148649229841 1102 88-89 0.1400336668267279 1102 90-91 -0.08564550647502145 1102 92-93 -0.04671815854428374 1102 94-95 0.7036224928965389 1102 96-97 0.9050978193092334 1102 98-99 0.8152285339098988 1102 100-101 0.4673352910048969 1103 1 0.23743985908943444 1103 2 0.24100279189374163 1103 3 0.1669624708784454 1103 4 0.2521235882003907 1103 5 -0.47076398619228854 1103 6 0.10712947974721487 1103 7 -0.17050384269676755 1103 8 0.16301626024782223 1103 9 -0.2142103131703621 1103 10-11 0.05708969825818855 1103 12-13 0.24735595532870747 1103 14-15 -0.04709495515465534 1103 16-17 -0.065166612414842 1103 18-19 -0.008849162529756427 1103 20-21 0.307814635056296 1103 22-23 0.1798071386951321 1103 24-25 0.3308803085006673 1103 26-27 0.5951394022517249 1103 28-29 0.5168497525911775 1103 30-31 0.5642764725425238 1103 32-33 0.1064041091131287 1103 34-35 0.34040663409803784 1103 36-37 0.1994510190476646 1103 38-39 0.4406477434089524 1103 40-41 -0.27863583538304226 1103 42-43 -0.01855401728939654 1103 44-45 -0.18488604256398133 1103 46-47 0.20263252775855278 1103 48-49 1.017228085994283 1103 50-51 1.2870303458660146 1103 52-53 1.4853889540020546 1103 54-55 0.5557884568525893 1103 56-57 0.3732107841835628 1103 58-59 0.2511661739861992 1103 60-61 0.1338653295576293 1103 62-63 -0.11512027404115699 1103 64-65 0.6668977495644697 1103 66-67 0.7116253079349626 1103 68-69 0.8722597899608644 1103 70-71 0.4485841968148314 1103 72-73 1.1209322815568186 1103 74-75 1.0592600567224935 1103 76-77 1.2842736590714203 1103 78-79 1.1721946982808866 1103 80-81 1.0229153933619806 1103 82-83 1.4868827398794124 1103 84-85 1.6151553322365935 1103 86-87 1.3425470009873592 1103 88-89 1.8277526856225244 1103 90-91 1.449158571256742 1103 92-93 1.9576622173716167 1103 94-95 0.9494810465760573 1103 96-97 1.2842790872289562 1103 98-99 2.6075190722979222 1103 100-101 3.1499571450985577 1104 1 -0.10940945597906193 1104 2 -0.41799264189621255 1104 3 -0.263174515422925 1104 4 -0.1984700191055495 1104 5 -0.20683704555301574 1104 6 -0.31387508646282924 1104 7 -0.10310658242279658 1104 8 -0.23241752970651675 1104 9 -0.3636167058644233 1104 10-11 0.11553718684265846 1104 12-13 -0.0445161907900129 1104 14-15 -0.28627303734643306 1104 16-17 0.08433110448014247 1104 18-19 -0.00948843193615545 1104 20-21 -0.6832355932542029 1104 22-23 -0.8843024503459631 1104 24-25 -1.2700329335084604 1104 26-27 -1.5068697301683684 1104 28-29 -1.5045201104225256 1104 30-31 -1.8541710160419456 1104 32-33 -1.6240068497909874 1104 34-35 -1.8395933659019619 1104 36-37 -1.002558113372423 1104 38-39 -1.0478454072759789 1104 40-41 -1.0695034152917273 1104 42-43 -0.6917047022209033 1104 44-45 -0.48680385078315425 1104 46-47 -0.448509024752866 1104 48-49 0.08836963850570356 1104 50-51 0.5110486107061973 1104 52-53 1.0669414654175782 1104 54-55 0.7959711052544165 1104 56-57 0.29375872938904735 1104 58-59 0.5967369502419118 1104 60-61 0.17998405101881332 1104 62-63 0.5105418264154657 1104 64-65 0.6012356491078492 1104 66-67 0.43153398373404883 1104 68-69 0.7025794246640622 1104 70-71 0.8841093109700822 1104 72-73 1.2648592221960868 1104 74-75 0.2909495544393934 1104 76-77 0.8566937503956211 1104 78-79 0.6594093101530341 1104 80-81 -0.1720617755877889 1104 82-83 0.24806995449128522 1104 84-85 0.2865708573507497 1104 86-87 0.6335059050969498 1104 88-89 -0.0448500541035024 1104 90-91 0.8773777493389296 1104 92-93 0.472274089517736 1104 94-95 -0.04978835981663465 1104 96-97 0.17943890458055378 1104 98-99 0.696468843987418 1104 100-101 0.6233818026328031 1105 1 0.00201911544951372 1105 2 -0.08561168951526099 1105 3 0.07968262743421661 1105 4 -0.21656525720078434 1105 5 -0.13064656936253982 1105 6 -0.3948274674152117 1105 7 0.014036274720062636 1105 8 0.04853485124586854 1105 9 -0.31695003919775644 1105 10-11 0.20601337731884684 1105 12-13 0.23072190444808172 1105 14-15 0.5265841055107074 1105 16-17 0.262426342575381 1105 18-19 0.5243210918733681 1105 20-21 0.15057393055531776 1105 22-23 0.3114118353683182 1105 24-25 0.10377659030106656 1105 26-27 0.6340826507840163 1105 28-29 0.6511941752917565 1105 30-31 0.9510670791961502 1105 32-33 1.0731360073518772 1105 34-35 0.9832637769551766 1105 36-37 1.1955371247228115 1105 38-39 0.8864403070097353 1105 40-41 1.078115632327325 1105 42-43 1.3811524406362352 1105 44-45 1.371767577788269 1105 46-47 0.6348243085804626 1105 48-49 1.1850363051723676 1105 50-51 0.1662867059442874 1105 52-53 0.2650367035128198 1105 54-55 1.0769234862068018 1105 56-57 1.2294730151033306 1105 58-59 1.4243559978609568 1105 60-61 1.070460241495006 1105 62-63 1.782922778796415 1105 64-65 1.983140411012613 1105 66-67 2.658200650400719 1105 68-69 2.3878175199021534 1105 70-71 2.130775977636752 1105 72-73 1.911049698386563 1105 74-75 1.7904733639632013 1105 76-77 1.3428842265860972 1105 78-79 1.292742643486367 1105 80-81 1.6755572720312593 1105 82-83 0.8242604306817611 1105 84-85 1.207523238303132 1105 86-87 1.6463630479540896 1105 88-89 1.9980070887536385 1105 90-91 2.23594917791036 1105 92-93 1.8270359942796368 1105 94-95 1.8483068782786027 1105 96-97 1.9670579521995997 1105 98-99 1.3931355106540835 1105 100-101 2.689096088347089 1106 1 -0.03726659883620442 1106 2 -0.2570402609438318 1106 3 -0.5881745154229279 1106 4 -0.7344224000579302 1106 5 -0.18064656936253698 1106 6 -0.4126846102723505 1106 7 -0.17882086813708042 1106 8 -0.13003657732556206 1106 9 0.09376424651652826 1106 10-11 0.07029909160456072 1106 12-13 -0.09963523840906419 1106 14-15 0.037298391224993566 1106 16-17 -0.16435937171033288 1106 18-19 -0.050678908126627675 1106 20-21 -0.017283212301819617 1106 22-23 -0.4207310217745359 1106 24-25 -0.5962234096989363 1106 26-27 -0.3373459206445588 1106 28-29 -0.23094868185109618 1106 30-31 0.2385670791961516 1106 32-33 -0.06079256407669931 1106 34-35 -0.882807651616254 1106 36-37 -2.0991057324200497 1106 38-39 -2.1814168358474078 1106 40-41 -2.0433129391012486 1106 42-43 -2.6367047022209036 1106 44-45 -3.042518136497442 1106 46-47 -3.0276756914195317 1106 48-49 -2.2078208376847783 1106 50-51 -3.0087132940557133 1106 52-53 -2.9563918679157553 1106 54-55 -2.4820050852217754 1106 56-57 -2.6687412706109583 1106 58-59 -3.406001144996189 1106 60-61 -3.4223969013621343 1106 62-63 -3.0992200783464376 1106 64-65 -2.840073874701666 1106 66-67 -2.2650136353135686 1106 68-69 -2.071111051526419 1106 70-71 -1.6924383080775343 1106 72-73 -2.026450301613437 1106 74-75 -1.645240921751089 1106 76-77 -1.4731872019853292 1106 78-79 -2.387614499370777 1106 80-81 -2.431585585111602 1106 82-83 -1.6025252836039527 1106 84-85 -1.403191047411152 1106 86-87 -1.514351237760195 1106 88-89 -1.4216357683892191 1106 90-91 -2.394407964946783 1106 92-93 -1.5122497200060785 1106 94-95 -1.6570502645785403 1106 96-97 -1.2722277620861142 1106 98-99 -1.1372216322030582 1106 100-101 -0.7841181973671958 1107 1 0.21446151176287742 1107 2 0.18996434735110057 1107 3 0.10134161360932836 1107 4 -0.01610442770769538 1107 5 0.15230273939322103 1107 6 -0.27731594667788073 1107 7 0.24399019177075587 1107 8 -0.0044605404591990805 1107 9 0.21530802531837168 1107 10-11 -0.3720972217594962 1107 12-13 -0.052688233800772366 1107 14-15 -0.4759504567012769 1107 16-17 -0.11222803530480974 1107 18-19 -0.048950797527552936 1107 20-21 0.15956010567052914 1107 22-23 0.24343948513790536 1107 24-25 -0.21143078297083662 1107 26-27 -1.10923532156621 1107 28-29 -0.9296814007451104 1107 30-31 -1.4420204784075352 1107 32-33 -1.009813301403888 1107 34-35 -0.7402385271922824 1107 36-37 -0.9565366079960782 1107 38-39 -0.20849056856629744 1107 40-41 0.4347976599771002 1107 42-43 -0.3504143796402559 1107 44-45 -0.434223205621862 1107 46-47 -0.5700144010969552 1107 48-49 -0.34722175934376054 1107 50-51 0.30061850317930805 1107 52-53 -0.1338112227544599 1107 54-55 0.8287668041791463 1107 56-57 0.4663393745503299 1107 58-59 -0.03992971642475851 1107 60-61 1.0729948037069903 1107 62-63 1.5488213963079396 1107 64-65 1.3819883372798927 1107 66-67 1.8291683923362037 1107 68-69 2.0334396397178303 1107 70-71 2.0227114615077184 1107 72-73 2.1472248135939367 1107 74-75 2.142777511428637 1107 76-77 1.59680127727734 1107 78-79 1.8217749015508815 1107 80-81 1.243529622261672 1107 82-83 1.2894678039536487 1107 84-85 2.3980762336948374 1107 86-87 2.463183324451787 1107 88-89 2.4599886555739126 1107 90-91 2.6444745235324802 1107 92-93 2.1346396809155834 1107 94-95 3.178491210075837 1107 96-97 3.3205141733977577 1107 98-99 2.435070994525052 1107 100-101 2.838865673600548 1108 1 -0.09991793055532838 1108 2 0.02019944849926958 1108 3 -0.14114061711784132 1108 4 -0.5349671942467893 1108 5 -0.34469015289764826 1108 6 -0.3330841260108528 1108 7 -0.3973438705583874 1108 8 -0.5555813715144211 1108 9 -0.32542461546893975 1108 10-11 -0.8951972764341818 1108 12-13 -0.485985117343688 1108 14-15 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-2.861799349599284 2316 68-69 -2.710753908669272 2316 70-71 -2.3892240223632513 2316 72-73 -2.041807444470578 2316 74-75 -2.7023837788939424 2316 76-77 -2.089972916271048 2316 78-79 -1.8672573565136368 2316 80-81 -2.0587284422544556 2316 82-83 -0.6985967121753838 2316 84-85 -0.6467624759825839 2316 86-87 -0.7464940949030527 2316 88-89 -0.7048500541035061 2316 90-91 0.07737774933893249 2316 92-93 -0.9743925771489295 2316 94-95 -0.9431216931499726 2316 96-97 -1.2672277620861152 2316 98-99 -1.0768644893459154 2316 100-101 -0.6766181973671941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 5.0 7 10.0 8 23.0 9 36.0 10 87.0 11 142.0 12 249.0 13 409.0 14 489.0 15 813.0 16 1092.0 17 1516.0 18 1422.0 19 815.0 20 639.0 21 619.0 22 678.0 23 639.0 24 655.0 25 663.0 26 671.0 27 755.0 28 899.0 29 1104.0 30 1302.0 31 1547.0 32 1934.0 33 2543.0 34 3471.0 35 5341.0 36 7511.0 37 12876.0 38 13705.0 39 1733.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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34.113075109768864 17.445529790102352 44-45 18.264176519316212 30.294449883274343 33.66593870020333 17.775434897206114 46-47 18.764684619555393 30.7834809325863 32.703024278571 17.748810169287307 48-49 18.839990360865112 30.491294656304596 32.6495572022411 18.019157780589193 50-51 18.510904271341648 31.126122055545512 32.26248569190915 18.100487981203685 52-53 18.82718838484246 31.56967287185975 31.392704379781915 18.210434363515873 54-55 18.364811133200796 30.940869931923608 31.558377010663293 19.1359419242123 56-57 18.824176155190074 31.127628170371707 31.17582384480993 18.87237182962829 58-59 19.041809747575154 30.333152599554193 31.50039158985481 19.12464606301584 60-61 18.41375986505211 30.822639918067352 32.09455388878848 18.669046328092055 62-63 18.116302186878727 30.85351527200434 32.24215314175552 18.78802939936141 64-65 17.89490330742816 31.243599011988678 31.89198144466534 18.969516235917826 66-67 17.99882523043557 30.80682571239231 31.28275799746973 19.911591059702392 68-69 18.079402373636967 30.33917705885897 31.853575516597388 19.72784505090668 70-71 18.434845472618832 30.464184589433096 31.49286101572384 19.60810892222423 72-73 18.265407554671967 29.386559431291044 32.14500873546599 20.203024278571 74-75 17.626061810952468 30.553045364178566 31.099765046087114 20.721127778781852 76-77 18.106512440508464 29.297698656545574 31.853575516597388 20.742213386348578 78-79 17.756340743418278 29.378275799746973 31.80010844026749 21.065275016567263 80-81 17.906952226037713 29.220133742996566 31.230797035966024 21.6421169949997 82-83 17.751822398939694 29.63506837761311 31.515452738116757 21.09765648533044 84-85 17.72785806266991 29.357411270342116 31.066111408152665 21.848619258835313 86-87 17.436291342851977 29.73296584131574 30.483764082173625 22.346978733658656 88-89 17.978492680281942 29.223145972648958 29.965660581962766 22.83270076510633 90-91 18.084801928001205 28.656424160265097 30.622081638801024 22.63669227293267 92-93 17.771184143145465 28.88965870409977 30.434225984275688 22.904931168479077 94-95 17.662208566781132 28.951292246520875 29.99804205072595 23.388457135972047 96-97 17.47093198385445 29.241972407976384 30.053015241882044 23.234080366287127 98-99 17.520633773118863 29.516838363756854 29.00174709319839 23.9607807699259 100-101 17.101348857876623 29.72232054767697 28.881073061252156 24.295257533194256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 551.0 1 352.0 2 161.5 3 167.0 4 145.5 5 134.5 6 203.0 7 331.5 8 402.5 9 410.0 10 354.0 11 274.0 12 249.5 13 244.0 14 247.0 15 251.0 16 247.5 17 251.0 18 276.5 19 294.5 20 303.0 21 349.5 22 396.0 23 423.5 24 494.5 25 559.0 26 658.0 27 833.5 28 1028.5 29 1225.0 30 1386.0 31 1557.0 32 1811.5 33 2059.5 34 2252.0 35 2395.0 36 2431.5 37 2448.0 38 2393.5 39 2290.5 40 2260.5 41 2180.0 42 2063.5 43 2053.0 44 2029.5 45 2016.5 46 1988.0 47 2140.0 48 2989.0 49 2919.5 50 2082.0 51 1700.0 52 1290.5 53 1051.5 54 935.5 55 811.0 56 678.5 57 565.0 58 478.0 59 431.5 60 343.5 61 250.5 62 183.0 63 111.5 64 72.5 65 51.5 66 33.5 67 24.5 68 16.5 69 14.0 70 15.5 71 11.5 72 9.0 73 8.0 74 6.5 75 3.5 76 2.0 77 2.5 78 2.0 79 2.0 80 1.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08434243026688355 2 0.0 3 0.0 4 0.0 5 0.001506114826194349 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006024459304777396 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.001506114826194349 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009789746370263268 44-45 0.001506114826194349 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 7.530574130971745E-4 86-87 0.0 88-89 0.0 90-91 0.009036688957166094 92-93 0.003012229652388698 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.009789746370263268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 66396.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.62286884752093 #Duplication Level Percentage of deduplicated Percentage of total 1 83.55746148647074 53.997228748719806 2 9.832894399515231 12.70859690342792 3 3.041461766145384 5.896439544550876 4 1.3447689188244343 3.4761130188565574 5 0.675880392476752 2.183866497981806 6 0.3962057473139581 1.536237122718236 7 0.2610296688186077 1.1807940237363697 8 0.16780478709767638 0.867522139887945 9 0.10487799193604774 0.6099765046087113 >10 0.5360430698953551 6.104283390565697 >50 0.03728995268837253 1.6763058015543106 >100 0.03495933064534924 4.209590939213205 >500 0.006991866129069849 3.9731309115006925 >1k 0.002330622043023283 1.5799144526778723 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1049 1.5799144526778723 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 997 1.501596481715766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 1.4217723959274655 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 697 1.0497620338574614 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 427 0.6431110307849871 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 409 0.6160009639134888 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 249 0.3750225917223929 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 236 0.3554430989818664 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 187 0.28164347249834326 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 172 0.259051750105428 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 167 0.25152117597445633 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 151 0.22742333875534673 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.19579492740526538 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 126 0.18977046810048798 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 117 0.17621543466473885 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 110 0.16567263088137837 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 107 0.16115428640279533 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 106 0.159648171576601 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 101 0.15211759744562925 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 99 0.14910536779324055 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 90 0.1355503343574914 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.129525875052714 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 78 0.11747695644315921 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 76 0.11446472679077054 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 69 0.10392192300741009 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 69 0.10392192300741009 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 67 0.10090969335502138 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 67 0.10090969335502138 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.530574130971745E-4 0.0 16-17 0.0 0.0 0.0 0.001506114826194349 0.0 18-19 0.0 0.0 0.0 0.001506114826194349 0.0 20-21 0.0 0.0 0.0 0.003012229652388698 0.0 22-23 0.0 0.0 0.0 0.0067775167178745705 0.0 24-25 0.0 0.0 0.0 0.014308090848846315 0.0 26-27 0.0 0.0 0.0 0.018826435327429364 0.0 28-29 0.0 0.0 0.0 0.05196096150370504 0.0 30-31 0.0 0.0 0.0 0.10994638231218748 0.0 32-33 0.0 0.0 0.0 0.1807337791433219 0.0 34-35 0.0 0.0 0.0 0.23269474064702694 0.0 36-37 0.0 0.0 0.0 0.2748659557804687 0.0 38-39 0.0 0.0 0.0 0.30950659678293874 0.0 40-41 0.0 0.0 0.0 0.3313452617627568 0.0 42-43 0.0 0.0 0.0 0.36447978793903246 0.0 44-45 0.0 0.0 0.0 0.3953551418760166 0.0 46-47 0.0 0.0 0.0 0.4427977589011386 0.0 48-49 0.0 0.0 0.0 0.5060545816013012 0.0 50-51 0.0 0.0 0.0 0.5617808301704922 0.0 52-53 0.0 0.0 0.0 0.7116392553768299 0.0 54-55 0.0 0.0 0.0 0.8871016326284715 0.0 56-57 0.0 0.0 0.0 1.1190433158624014 0.0 58-59 0.0 0.0 0.0 1.3660461473582746 0.0 60-61 0.0 0.0 0.0 1.7448340261461532 0.0 62-63 0.0 0.0 0.0 2.0227122115790106 0.0 64-65 0.0 0.0 0.0 2.3209229471654917 0.0 66-67 0.0 0.0 0.0 2.783300198807157 0.0 68-69 0.0 0.0 0.0 3.352611603108621 0.0 70-71 0.0 0.0 0.0 3.8564070124706307 0.0 72-73 0.0 0.0 0.0 4.339116814265919 0.0 74-75 0.0 0.0 0.0 5.206638954153865 0.0 76-77 0.0 0.0 0.0 5.806072654979216 0.0 78-79 0.0 0.0 0.0 6.244352069401771 0.0 80-81 0.0 0.0 0.0 6.678113139345744 0.0 82-83 0.0 0.0 0.0 7.398789083679739 0.0 84-85 0.0 0.0 0.0 8.21435026206398 0.0 86-87 0.0 0.0 0.0 9.177510693415265 0.0 88-89 0.0 0.0 0.0 10.566901620579554 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATAGA 20 0.002065447 71.25 1 GAGTAAT 20 0.002065447 71.25 1 CCCTATG 25 0.005000502 57.0 2 GTATAGC 45 9.55954E-4 42.222225 1 CATGGGC 85 1.6092381E-5 33.52941 4 GGGCTTG 60 0.0039244094 31.666668 7 CGGGGGG 30 0.003938479 31.666668 54-55 CACAGAA 60 0.0039244094 31.666668 2 CAGAACT 70 0.008335699 27.142859 4 GATTTAG 35 0.008365315 27.142859 26-27 GTTACCC 35 0.008365315 27.142859 12-13 ATGGGCT 90 8.8586344E-4 26.388891 5 TGGTTAC 45 8.9057337E-4 26.388891 10-11 GAGTACC 50 0.0016459752 23.75 10-11 GAGTACT 690 0.0 23.405796 12-13 AGTACTT 690 0.0 22.373188 12-13 GTACTTT 725 0.0 22.275864 14-15 AAGAGGC 120 5.809852E-9 21.770834 94-95 ACTTTTT 750 0.0 21.533333 16-17 TACTTTT 775 0.0 19.612902 14-15 >>END_MODULE