FastQCFastQC Report
Thu 26 May 2016
SRR937323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124245
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.8330315103223469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.7058634150267616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.4169181858424886No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2570.20684937019598373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.18189866795444487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1850.14889935208660307No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1710.13763129300977905No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1710.13763129300977905No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1450.11670489758139159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.047.5357131
GTATCAA9000.044.366671
TATACAG802.2347194E-741.5769735
TACCTGG651.3186554E-436.551182
ATGGGAC801.0673288E-535.6374055
TAGGTTG550.002566407734.5574845
ATCAACG11450.034.44433
TATCAAC11500.034.2945482
CAACGCA11550.034.1460885
TCAACGC11600.033.99894
AACGCAG11650.033.8529856
ATGTAAG600.00392301831.6904771
AGGGTAG600.0039307831.677695
GCACCCG600.0039307831.677699
CGCAGAG13000.029.9719728
GTACATG6200.029.9014991
ACGCAGA13100.029.7431767
GTCCTGG804.4511547E-429.7098221
GCAGAGT13150.029.6300859
TCCATAT650.00580288129.2527471