##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937323_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124245 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00596402269709 33.0 31.0 34.0 31.0 34.0 2 32.136721799670006 33.0 31.0 34.0 31.0 34.0 3 32.14373214213852 34.0 31.0 34.0 30.0 34.0 4 35.757076743530924 37.0 35.0 37.0 35.0 37.0 5 35.7610769045032 37.0 35.0 37.0 35.0 37.0 6 35.817232081773916 37.0 36.0 37.0 35.0 37.0 7 35.783967161656406 37.0 35.0 37.0 35.0 37.0 8 35.77239325526178 37.0 35.0 37.0 35.0 37.0 9 37.417747193046 39.0 37.0 39.0 35.0 39.0 10-11 37.34160730814117 39.0 37.0 39.0 35.0 39.0 12-13 37.23029095738259 39.0 37.0 39.0 34.5 39.0 14-15 38.36224797778583 40.0 38.0 41.0 34.0 41.0 16-17 38.29882892671738 40.0 38.0 41.0 34.0 41.0 18-19 38.29714676646948 40.0 38.0 41.0 34.0 41.0 20-21 38.2213569962574 40.0 38.0 41.0 34.0 41.0 22-23 38.277415590164594 40.0 38.0 41.0 34.0 41.0 24-25 38.25146283552658 40.0 38.0 41.0 34.0 41.0 26-27 38.15225964827559 40.0 38.0 41.0 34.0 41.0 28-29 37.999573423477806 40.0 38.0 41.0 34.0 41.0 30-31 37.695247293653665 40.0 37.5 41.0 33.0 41.0 32-33 37.39863575999034 40.0 36.5 41.0 33.0 41.0 34-35 37.37211155378486 40.0 37.0 41.0 33.0 41.0 36-37 37.21271278522275 40.0 36.5 41.0 32.5 41.0 38-39 37.05918145599421 40.0 36.0 41.0 32.0 41.0 40-41 36.80307859471206 40.0 36.0 41.0 31.5 41.0 42-43 36.47428467946396 39.0 36.0 41.0 30.5 41.0 44-45 36.09810857579782 39.0 35.0 41.0 30.0 41.0 46-47 35.50963419051068 39.0 35.0 41.0 27.0 41.0 48-49 35.05131796048131 39.0 35.0 40.0 24.5 41.0 50-51 34.061141293412206 38.0 34.0 39.5 22.5 40.5 52-53 34.13040363797336 38.0 34.5 39.5 22.5 40.5 54-55 34.406241699867195 39.0 35.0 40.0 21.0 41.0 56-57 34.14675842086201 38.0 34.0 40.0 18.5 41.0 58-59 33.87836935087931 38.0 34.0 40.0 14.0 41.0 60-61 33.545502837136304 38.0 34.0 40.0 9.5 41.0 62-63 33.169841844742244 38.0 33.0 40.0 7.5 41.0 64-65 32.768228097710164 37.0 33.0 40.0 2.0 41.0 66-67 32.30591573101533 36.5 32.0 39.5 2.0 41.0 68-69 31.810217714998593 36.0 31.5 39.0 2.0 40.5 70-71 31.326214334580868 36.0 31.0 39.0 2.0 40.0 72-73 30.854686305283913 35.0 31.0 38.5 2.0 40.0 74-75 30.315417119401182 35.0 30.0 37.5 2.0 39.5 76-77 29.81570686949173 35.0 29.5 37.0 2.0 39.0 78-79 29.281769890136424 34.0 29.0 36.5 2.0 39.0 80-81 28.72776369270393 34.0 28.5 36.0 2.0 38.5 82-83 28.19766590204837 34.0 27.5 35.5 2.0 37.0 84-85 27.656384562759065 34.0 26.0 35.0 2.0 37.0 86-87 27.107553623888286 33.5 25.5 35.0 2.0 36.0 88-89 26.55041651575516 33.0 25.0 35.0 2.0 36.0 90-91 26.00522355024347 32.5 23.5 35.0 2.0 35.5 92-93 25.38109783089863 32.0 19.5 35.0 2.0 35.0 94-95 24.65137430077669 32.0 14.0 34.0 2.0 35.0 96-97 23.808764940239044 31.0 2.0 34.0 2.0 35.0 98-99 22.637848605577688 31.0 2.0 34.0 2.0 35.0 100-101 19.60560183508391 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.08359983784986369 1101 2 -0.10430984294223933 1101 3 -0.08417629487771094 1101 4 -0.1126941323900823 1101 5 -0.09363585980631939 1101 6 0.21523724289175306 1101 7 0.0683231250753451 1101 8 0.17288616150986513 1101 9 -0.03301346380129644 1101 10-11 -0.05488935295813491 1101 12-13 0.4014773086989649 1101 14-15 0.36338320095493515 1101 16-17 0.23650431693858565 1101 18-19 -0.07302348512477153 1101 20-21 -0.04725667160445113 1101 22-23 -0.14176848981750823 1101 24-25 -0.13902306615489124 1101 26-27 -0.07712167477678378 1101 28-29 -0.23442424804564865 1101 30-31 0.03195946954078721 1101 32-33 -0.2863304351881055 1101 34-35 -0.17846149298090808 1101 36-37 0.14232221839007053 1101 38-39 0.10110289631764857 1101 40-41 0.41405218912018427 1101 42-43 0.4372309790152187 1101 44-45 0.7185294267504645 1101 46-47 0.8025947927438679 1101 48-49 0.9323795641419821 1101 50-51 0.9063343692414847 1101 52-53 1.0406824725881663 1101 54-55 1.3038518152528908 1101 56-57 1.4107594558293215 1101 58-59 1.0333911599227292 1101 60-61 1.2542796625398012 1101 62-63 1.4914946576935009 1101 64-65 1.2817503339357579 1101 66-67 1.462831336375313 1101 68-69 1.0012816862607785 1101 70-71 1.5524804339211578 1101 72-73 1.4755064146404955 1101 74-75 0.928390233091207 1101 76-77 0.6133128080514361 1101 78-79 0.9570184714690342 1101 80-81 1.1807987931098474 1101 82-83 1.4161088612639823 1101 84-85 1.2818770326172988 1101 86-87 1.2625776947265415 1101 88-89 0.7740045350635647 1101 90-91 0.015818233668955628 1101 92-93 -0.03608438325302643 1101 94-95 -0.21337788348086306 1101 96-97 -0.09501447481452274 1101 98-99 1.0047998808687275 1101 100-101 0.8813066026839174 1102 1 -0.11244599169601699 1102 2 0.12254482412981815 1102 3 0.14655493041873058 1102 4 0.29335633887777846 1102 5 0.0816833861651034 1102 6 0.22219224441197127 1102 7 0.23056848840766975 1102 8 -0.10455378984312347 1102 9 0.28425622303360853 1102 10-11 -0.08732702398275904 1102 12-13 0.41591938835843223 1102 14-15 0.504136469425589 1102 16-17 0.4738294917637589 1102 18-19 0.6376408505395617 1102 20-21 0.21034442295316325 1102 22-23 0.3847023219793897 1102 24-25 0.38271606427989013 1102 26-27 0.38475883601677197 1102 28-29 0.4552990721124459 1102 30-31 0.40967686084513133 1102 32-33 0.6744289141582058 1102 34-35 0.9501110214611757 1102 36-37 1.083717779350856 1102 38-39 0.7323167911184996 1102 40-41 1.119452766803363 1102 42-43 1.462466612946507 1102 44-45 1.2666063498273843 1102 46-47 1.8687433181315214 1102 48-49 2.1155279375077427 1102 50-51 1.815577300223545 1102 52-53 2.064682928653845 1102 54-55 2.5699281302422534 1102 56-57 2.7357062481674177 1102 58-59 2.5303317193632893 1102 60-61 2.4197364883227195 1102 62-63 2.430704903968966 1102 64-65 2.4209453780221075 1102 66-67 2.4584417042682922 1102 68-69 2.7711585485289447 1102 70-71 3.0865903457484585 1102 72-73 2.9011677098670106 1102 74-75 2.787541950938575 1102 76-77 3.2748740728735726 1102 78-79 2.9203431902285395 1102 80-81 3.101519376873906 1102 82-83 3.2383382622977344 1102 84-85 2.576381441252142 1102 86-87 2.520938898739921 1102 88-89 3.2172433614545675 1102 90-91 3.5594295136932814 1102 92-93 3.4922676994468844 1102 94-95 3.1239206875133583 1102 96-97 3.29454162126331 1102 98-99 3.5368384944290803 1102 100-101 4.532568384380486 1103 1 0.3904567659237195 1103 2 0.1884506164507087 1103 3 0.7288876919967748 1103 4 0.35695311945568875 1103 5 0.42143797120270676 1103 6 0.415093682596428 1103 7 0.5698612710966771 1103 8 0.3418771377198766 1103 9 0.15976750420526287 1103 10-11 0.21597816139789217 1103 12-13 0.4796151265824733 1103 14-15 0.17147384902712304 1103 16-17 0.2376835622215978 1103 18-19 0.5236746280827731 1103 20-21 0.45787048179997925 1103 22-23 0.1865231426681362 1103 24-25 -0.11728393572010987 1103 26-27 0.7751137641075516 1103 28-29 0.6010966707402403 1103 30-31 0.33420516273192646 1103 32-33 0.24940582404077105 1103 34-35 0.04682562760974207 1103 36-37 -0.02810436077317746 1103 38-39 0.3891976461125566 1103 40-41 0.9361502202932712 1103 42-43 0.5816116188839615 1103 44-45 0.21145395505234887 1103 46-47 0.3911407320383731 1103 48-49 1.1685362499500158 1103 50-51 0.20596521419636105 1103 52-53 -0.06488561600904319 1103 54-55 0.557030650363636 1103 56-57 0.8410301695290769 1103 58-59 0.7444760655831004 1103 60-61 0.6320995969122336 1103 62-63 0.9684737389076119 1103 64-65 0.840558783484564 1103 66-67 0.6783255939635033 1103 68-69 0.9763637207152485 1103 70-71 1.5303824027480637 1103 72-73 1.512627005288568 1103 74-75 1.506733137110892 1103 76-77 0.9433989442286297 1103 78-79 0.988294107236058 1103 80-81 0.2935961680073014 1103 82-83 0.7446055798858033 1103 84-85 0.21225028938514257 1103 86-87 0.41680246913179175 1103 88-89 0.5662010075820234 1103 90-91 0.8267800876476272 1103 92-93 0.1681814083794606 1103 94-95 0.5843046431803351 1103 96-97 -0.26503088170541744 1103 98-99 0.6390287569966979 1103 100-101 1.7026765780735822 1104 1 -0.2685998378498624 1104 2 0.0837336353186302 1104 3 0.32473674860055013 1104 4 -0.22965065412921604 1104 5 -0.28950542502371235 1104 6 -0.20584971362998772 1104 7 0.24854051637969832 1104 8 -0.27189644718578165 1104 9 -0.039100420323038065 1104 10-11 -0.08260674426248471 1104 12-13 -0.19047921304016313 1104 14-15 0.15892667921579573 1104 16-17 -0.24599568306141606 1104 18-19 -0.23802348512477067 1104 20-21 -0.11486536725661978 1104 22-23 0.1352967275737953 1104 24-25 -0.47228393572011385 1104 26-27 -0.7323390660811313 1104 28-29 -0.6483372915239158 1104 30-31 -0.46532313915486867 1104 32-33 -0.7686130438837537 1104 34-35 -0.33128757993742397 1104 36-37 -0.2587647381316671 1104 38-39 0.11882028762199326 1104 40-41 0.26850871085930805 1104 42-43 0.0039701094500017575 1104 44-45 0.4685294267504645 1104 46-47 1.0644426188308174 1104 48-49 1.5654230424028484 1104 50-51 1.3756821953284373 1104 52-53 1.522095516066436 1104 54-55 1.5436344239485464 1104 56-57 1.7242377166988803 1104 58-59 1.8558911599227272 1104 60-61 2.2313448799311075 1104 62-63 1.9929077011717666 1104 64-65 1.6727285948053172 1104 66-67 1.6922878581144438 1104 68-69 1.1547599471303442 1104 70-71 1.8180239121820243 1104 72-73 1.0644194581187563 1104 74-75 1.1701293635259873 1104 76-77 1.6039649819644808 1104 78-79 1.533105427990776 1104 80-81 1.6334074887620211 1104 82-83 2.3968697308291986 1104 84-85 1.3597031195738225 1104 86-87 1.176708129509148 1104 88-89 1.565634969846176 1104 90-91 2.1931008423646077 1104 92-93 1.904785181964364 1104 94-95 2.1583612469539197 1104 96-97 2.551289873011566 1104 98-99 2.721104228694813 1104 100-101 1.9673935592056573 1105 1 0.4000958143240503 1105 2 0.5750379831447177 1105 3 0.6603889225135973 1105 4 0.5938276067403478 1105 5 0.49875544454150855 1105 6 0.7098024602830577 1105 7 0.5954970381188289 1105 8 0.716364422379435 1105 9 0.5756821883726104 1105 10-11 0.725001951389693 1105 12-13 0.8525642652207068 1105 14-15 0.6215353748679746 1105 16-17 1.005526056069023 1105 18-19 0.9432808627013216 1105 20-21 1.094047676221642 1105 22-23 0.49138368409553834 1105 24-25 0.9859769338451088 1105 26-27 1.3250522382667 1105 28-29 0.7655757519543513 1105 30-31 0.9477203391060058 1105 32-33 0.6511695648119016 1105 34-35 0.7726254635408409 1105 36-37 0.5934091749118195 1105 38-39 0.5222985484915554 1105 40-41 0.3787261021636539 1105 42-43 0.2035353268413047 1105 44-45 0.4793989919678552 1105 46-47 1.071616531874298 1105 48-49 1.0628143467506774 1105 50-51 0.5476387170675636 1105 52-53 0.6792694291099082 1105 54-55 0.7821126848181095 1105 56-57 1.405324673220619 1105 58-59 1.623065072966206 1105 60-61 1.6374318364528406 1105 62-63 1.537690309867422 1105 64-65 1.5833807687183672 1105 66-67 1.525331336375313 1105 68-69 1.6208469036520796 1105 70-71 2.006284781747244 1105 72-73 2.19289771898832 1105 74-75 2.0642597983085977 1105 76-77 2.0480954167470884 1105 78-79 1.625496732338604 1105 80-81 2.0557987931098474 1105 82-83 1.6253479916987672 1105 84-85 1.271007467399908 1105 86-87 0.7788820425526275 1105 88-89 1.1136784481070414 1105 90-91 1.3500573641037406 1105 92-93 1.2084808341382782 1105 94-95 1.623578638258266 1105 96-97 1.6278116121420005 1105 98-99 1.3852346634774229 1105 100-101 0.4030457331187023 1106 1 -0.00944729547698131 1106 2 -1.9306731443423857 1106 3 -1.1539073191960583 1106 4 -0.24202353548514566 1106 5 -0.3735732216338761 1106 6 -0.8883920865113453 1106 7 -0.605866263281321 1106 8 -0.7110489895586625 1106 9 -0.49469364066202104 1106 10-11 -1.4177762357879047 1106 12-13 -1.126157179141856 1106 14-15 -0.8623445072248757 1106 16-17 -0.5908261915359887 1106 18-19 -0.782769247836633 1106 20-21 -0.020543333358318705 1106 22-23 -0.6503812385278991 1106 24-25 -0.9986398679235009 1106 26-27 -0.059711947437058654 1106 28-29 -0.603252545761201 1106 30-31 0.14696499643834926 1106 32-33 -0.15649439981596203 1106 34-35 0.20464462345240264 1106 36-37 0.6509810245801972 1106 38-39 -0.1271119157678342 1106 40-41 0.04655955831694314 1106 42-43 0.0606650247042424 1106 44-45 -0.5293519291817432 1106 46-47 -0.32208280489799535 1106 48-49 -0.2033057711564723 1106 50-51 -0.21304661823088367 1106 52-53 -1.0173960093572987 1106 54-55 -1.3058571014751763 1106 56-57 -1.5161012663519635 1106 58-59 -1.7324986705857448 1106 60-61 -1.493739865831607 1106 62-63 -1.1958211123875557 1106 64-65 -1.6673561509573922 1106 66-67 -0.9894070571397897 1106 68-69 -1.347189205412029 1106 70-71 -3.372399816631532 1106 72-73 -2.3844788469659903 1106 74-75 -2.9354638568129943 1106 76-77 -3.095696034984673 1106 78-79 -1.5791827076024418 1106 80-81 -1.1171009858142504 1106 82-83 -1.1914353539165639 1106 84-85 -1.1775002702566866 1106 86-87 -2.468630853541697 1106 88-89 -2.123517572526705 1106 90-91 -1.0864754288218315 1106 92-93 -1.7286046485441098 1106 94-95 -1.9268082445715073 1106 96-97 -1.8350660591918277 1106 98-99 -2.118726279779761 1106 100-101 -2.0553183052011192 1107 1 0.10989331283506587 1107 2 -0.0936636249553402 1107 3 -0.10375640208438242 1107 4 -0.014719147279897982 1107 5 0.16090553388040263 1107 6 -0.18790450815053816 1107 7 -0.23461016855181072 1107 8 -0.529019734857016 1107 9 -0.12622370799427074 1107 10-11 -0.13637386755015513 1107 12-13 -0.17178058290318177 1107 14-15 -0.6627856495513242 1107 16-17 -0.9064751351162101 1107 18-19 -0.49254403306997574 1107 20-21 -0.8469886549278556 1107 22-23 -0.7165525874946965 1107 24-25 0.053948940992214034 1107 26-27 0.18129107090516783 1107 28-29 -0.0247756476882941 1107 30-31 -0.5149806734014462 1107 32-33 -0.38635276991114864 1107 34-35 -0.4869040182935933 1107 36-37 -0.5091072038850868 1107 38-39 -0.6273440959396552 1107 40-41 -0.7652584124283663 1107 42-43 -1.5889750960294506 1107 44-45 -1.2797582444824087 1107 46-47 -1.268913545552742 1107 48-49 -0.9371112041724956 1107 50-51 -1.3030164348085478 1107 52-53 -0.8958496894130192 1107 54-55 -1.2665025623528194 1107 56-57 -1.275214338095637 1107 58-59 -2.1318485661046687 1107 60-61 -1.9146825173291688 1107 62-63 -1.8748320248556318 1107 64-65 -2.930764555879609 1107 66-67 -1.558945018597882 1107 68-69 -1.7822948473902045 1107 70-71 -1.141496635763179 1107 72-73 -0.747840815853845 1107 74-75 -0.9779528282548355 1107 76-77 -0.7886377577615491 1107 78-79 -1.2460041610503225 1107 80-81 -1.3002911413749665 1107 82-83 -0.9232672554721688 1107 84-85 -1.1699544146727554 1107 86-87 -1.0222644732305746 1107 88-89 -1.4688855780990302 1107 90-91 -2.0639539521559414 1107 92-93 -1.7032970098164597 1107 94-95 -0.6796524516762155 1107 96-97 -0.5359019078103522 1107 98-99 -1.1398546754147745 1107 100-101 -0.16931876956146397 1108 1 0.019177939927914167 1108 2 -0.08848858690359052 1108 3 0.20918119304499783 1108 4 0.5003493458707879 1108 5 0.15938346386517566 1108 6 -0.20251638029665742 1108 7 -0.008126150286969391 1108 8 -0.2730075582968965 1108 9 -0.2702115314341498 1108 10-11 -0.6520511887069276 1108 12-13 -0.3299236574846134 1108 14-15 -0.7082955430064217 1108 16-17 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-0.2796017160090436 2316 96-97 -0.08333975661806647 2316 98-99 0.01184496943555402 2316 100-101 1.9527639295760295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 546.0 3 83.0 4 95.0 5 87.0 6 91.0 7 113.0 8 134.0 9 166.0 10 199.0 11 319.0 12 470.0 13 615.0 14 805.0 15 1052.0 16 1492.0 17 2172.0 18 1775.0 19 1099.0 20 985.0 21 987.0 22 1061.0 23 1123.0 24 1238.0 25 1430.0 26 1534.0 27 1727.0 28 1946.0 29 2286.0 30 2836.0 31 3440.0 32 4442.0 33 6105.0 34 8339.0 35 12018.0 36 17154.0 37 23702.0 38 18463.0 39 2115.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80307615335577 19.974822669281238 16.04247867593063 21.179622501432362 2 14.235926230698484 20.668957839198413 42.492376204075704 22.602739726027394 3 17.94052632428159 26.49047227197186 32.1740323023057 23.394969101440857 4 11.362279555318908 17.701728060409508 39.89221808090098 31.043774303370604 5 11.79327481605783 37.94936749709565 36.086549632115656 14.170808054730863 6 28.21644030067426 38.430331967609774 18.860696196406103 14.492531535309869 7 25.398835352378494 34.56761247237591 22.60739115706612 17.426161018179474 8 25.021575190547246 34.5606323345566 21.37274670323023 19.04504577166593 9 25.551943599712835 16.57323083624396 22.160021295302933 35.71480426874027 10-11 23.090667817543068 27.431865024785523 28.87946855324349 20.597998604427918 12-13 24.812054334849805 25.424289355660957 29.4005904559094 20.36306585357984 14-15 21.723805567766156 26.35756141037171 28.804700701314307 23.11393232054782 16-17 19.62657525960278 29.734852303659643 29.650972880330677 20.987599556406895 18-19 19.320747717815554 27.61725428211993 33.40859972258959 19.65339827747492 20-21 20.182753726046844 26.483563455706822 33.4473191818827 19.886363636363637 22-23 19.853385914402537 26.339728546197954 33.69180396616102 20.115081573238495 24-25 19.830155126597337 26.65499957659754 33.586299502804536 19.928545794000588 26-27 19.431294908912026 26.840912573285696 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52-53 19.791074273499103 28.664771008530458 30.799604735112023 20.74454998285841 54-55 19.42290242574557 28.178170306293225 31.204403847317163 21.194523420644042 56-57 19.836616856043808 28.252945493254195 30.874253042265103 21.03618460843689 58-59 20.012982614585688 27.659016869335222 31.184078960100635 21.143921555978455 60-61 19.720984617865856 27.575751466645166 31.75695018446465 20.946313731024333 62-63 19.530516053335052 27.457795231778466 31.94216525480108 21.0695234600854 64-65 19.560282715715882 27.27327707510997 31.863577168246493 21.302863040927654 66-67 19.21719951452983 27.25769837141694 31.47814376207123 22.046958351982003 68-69 19.178198180762532 27.157118895555126 31.951567640797368 21.713115282884978 70-71 19.41300517319936 27.101032623816074 31.6577220987948 21.828240104189767 72-73 19.244850889501784 26.741100949984677 31.66481185787326 22.34923630264028 74-75 19.055011431313332 26.984350995754085 31.591150107055157 22.36948746587743 76-77 19.09865879525457 26.316808206914903 31.9884993507698 22.59603364706072 78-79 19.057623291261745 25.9962095245776 32.07145449413283 22.874712690027824 80-81 18.951821026484726 26.19761927804123 31.95385335010807 22.89670634536598 82-83 19.05397450061692 26.41266743546527 32.025838084562466 22.50751997935534 84-85 18.837412763624194 26.41422713913068 31.206331415577765 23.54202868166736 86-87 18.926950028626493 26.41096354355662 31.25125996887373 23.410826458943156 88-89 18.778729375332656 25.187093756552315 31.738197771003694 24.295979097111335 90-91 18.724018499030294 25.60027095353873 31.953163745448826 23.72254680198215 92-93 18.67565584148515 25.455036652930218 31.87071072007484 23.998596785509793 94-95 18.24856661317506 25.52557516914368 32.02803067568766 24.197827541993597 96-97 18.267707010221983 26.452549446561424 31.67079981451239 23.608943728704208 98-99 18.79780803800046 26.744275132360475 29.85237724649895 24.605539583140118 100-101 19.2669945972099 26.223691637771147 29.64680267720345 24.8625110878155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 187.0 1 109.0 2 37.0 3 38.5 4 27.5 5 30.5 6 49.5 7 122.5 8 220.5 9 279.5 10 269.0 11 246.0 12 256.0 13 260.0 14 289.0 15 327.0 16 354.0 17 352.0 18 361.5 19 403.5 20 434.5 21 493.5 22 558.5 23 631.0 24 748.5 25 904.0 26 1165.0 27 1441.5 28 1620.5 29 1858.0 30 2069.0 31 2359.5 32 2798.0 33 3076.0 34 3414.0 35 3697.0 36 3977.5 37 4301.0 38 4349.0 39 4374.0 40 4348.5 41 4386.0 42 4459.0 43 4521.0 44 4624.0 45 4676.5 46 4664.0 47 4758.5 48 5144.0 49 4933.5 50 4202.5 51 3670.5 52 3260.0 53 2996.0 54 2738.0 55 2381.0 56 2001.5 57 1687.0 58 1457.0 59 1203.5 60 970.5 61 771.5 62 563.0 63 408.5 64 291.0 65 205.5 66 147.5 67 106.0 68 81.5 69 50.0 70 25.5 71 27.5 72 27.0 73 19.5 74 14.5 75 7.5 76 4.5 77 3.5 78 3.0 79 1.5 80 0.0 81 0.5 82 1.5 83 2.5 84 1.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26077508149221296 2 0.2342146565254135 3 0.2342146565254135 4 0.2342146565254135 5 0.23582437925067407 6 0.20684937019598373 7 0.2084590929212443 8 0.2092639542838746 9 0.22053201336069864 10-11 0.22656847358042578 12-13 0.21972715199806833 14-15 0.2120809690530806 16-17 0.20725180087729891 18-19 0.19397158839389916 20-21 0.2036299247454626 22-23 0.19799589520705058 24-25 0.2008129099762566 26-27 0.19638617248179 28-29 0.20604450883335346 30-31 0.2193247213167532 32-33 0.2112761076904503 34-35 0.20966638496518974 36-37 0.20242263270151714 38-39 0.19638617248179 40-41 0.1915570043060083 42-43 0.19839832588836576 44-45 0.2012153406575717 46-47 0.20483721678940803 48-49 0.2092639542838746 50-51 0.21610527586623207 52-53 0.22334902812990465 54-55 0.2112761076904503 56-57 0.19477644975652944 58-59 0.18753269749285684 60-61 0.19115457362469315 62-63 0.19034971226206285 64-65 0.18793512817417202 66-67 0.19437401907521432 68-69 0.190752142943378 70-71 0.19356915771258398 72-73 0.19558131111915975 74-75 0.19598374180047487 76-77 0.20282506338283232 78-79 0.2012153406575717 80-81 0.20041047929494146 82-83 0.19477644975652944 84-85 0.18391082136102055 86-87 0.18833755885548714 88-89 0.19558131111915975 90-91 0.19195943498732343 92-93 0.19638617248179 94-95 0.1915570043060083 96-97 0.199203187250996 98-99 0.19839832588836576 100-101 0.18914242021811742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 124245.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.56609923940601 #Duplication Level Percentage of deduplicated Percentage of total 1 90.62902087743328 70.29739627349188 2 5.612626073963392 8.706990220934443 3 1.4641182086083095 3.4069781480140047 4 0.7139003029925705 2.2149784699585497 5 0.43062300253185576 1.6700873274578456 6 0.23243265678827874 1.0817336713751058 7 0.20441622047897728 1.1099038190671657 8 0.13385630681110697 0.8306169262344562 9 0.07263520524633711 0.5070626584570808 >10 0.4793923546258249 6.338283230713509 >50 0.014527041049267421 0.6865467423236347 >100 0.009338812103100487 1.1936094007807156 >500 0.0020752915784667744 1.1227816008692504 >1k 0.0010376457892333872 0.8330315103223469 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.8330315103223469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 877 0.7058634150267616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 518 0.4169181858424886 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 257 0.20684937019598373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 226 0.18189866795444487 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 185 0.14889935208660307 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 171 0.13763129300977905 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 171 0.13763129300977905 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 145 0.11670489758139159 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.0243068131514347E-4 0.0 24-25 0.0 0.0 0.0 0.0032194454505211478 0.0 26-27 0.0 0.0 0.0 0.004426737494466578 0.0 28-29 0.0 0.0 0.0 0.007646182944987725 0.0 30-31 0.0 0.0 0.0 0.01408507384603002 0.0 32-33 0.0 0.0 0.0 0.020523964747072316 0.0 34-35 0.0 0.0 0.0 0.025755563604169182 0.0 36-37 0.0 0.0 0.0 0.034206607911787194 0.0 38-39 0.0 0.0 0.0 0.051108696527023226 0.0 40-41 0.0 0.0 0.0 0.06438890901042295 0.0 42-43 0.0 0.0 0.0 0.11227816008692502 0.0 44-45 8.048613626302869E-4 0.0 0.0 0.18028894522918426 0.0 46-47 0.0012072920439454304 0.0 0.0 0.24870216105275866 0.0 48-49 0.0016097227252605739 0.0 0.0 0.4036379733590889 0.0 50-51 0.0016097227252605739 0.0 0.0 0.535635236830456 0.0 52-53 0.0016097227252605739 0.0 0.0 0.7026439695762405 0.0 54-55 0.0016097227252605739 0.0 0.0 1.0362590043864945 0.0 56-57 0.0016097227252605739 0.0 0.0 1.380337236910942 0.0 58-59 0.0016097227252605739 0.0 0.0 1.8479616885991388 0.0 60-61 0.0016097227252605739 0.0 0.0 2.349792748199123 0.0 62-63 0.0016097227252605739 0.0 0.0 3.091874924544247 0.0 64-65 0.0020121534065757176 0.0 0.0 3.6375709284075817 0.0 66-67 0.002414584087890861 0.0 0.0 4.353495110467222 4.0243068131514347E-4 68-69 0.002414584087890861 0.0 0.0 5.183709606020363 8.048613626302869E-4 70-71 0.002414584087890861 0.0 0.0 5.905267817618416 8.048613626302869E-4 72-73 0.002414584087890861 0.0 0.0 7.0694997786631255 8.048613626302869E-4 74-75 0.002414584087890861 0.0 0.0 8.336753994124512 8.048613626302869E-4 76-77 0.002414584087890861 0.0 0.0 9.501388385850536 8.048613626302869E-4 78-79 0.002414584087890861 0.0 0.0 10.491770292567105 8.048613626302869E-4 80-81 0.002414584087890861 0.0 0.0 11.747354018270354 8.048613626302869E-4 82-83 0.002414584087890861 0.0 0.0 12.93492695883134 8.048613626302869E-4 84-85 0.002414584087890861 0.0 0.0 14.161535675479898 8.048613626302869E-4 86-87 0.002414584087890861 0.0 0.0 16.126604692341743 8.048613626302869E-4 88-89 0.002414584087890861 0.0 0.0 18.663930138033724 8.048613626302869E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 460 0.0 47.535713 1 GTATCAA 900 0.0 44.36667 1 TATACAG 80 2.2347194E-7 41.576973 5 TACCTGG 65 1.3186554E-4 36.55118 2 ATGGGAC 80 1.0673288E-5 35.637405 5 TAGGTTG 55 0.0025664077 34.557484 5 ATCAACG 1145 0.0 34.4443 3 TATCAAC 1150 0.0 34.294548 2 CAACGCA 1155 0.0 34.146088 5 TCAACGC 1160 0.0 33.9989 4 AACGCAG 1165 0.0 33.852985 6 ATGTAAG 60 0.003923018 31.690477 1 AGGGTAG 60 0.00393078 31.67769 5 GCACCCG 60 0.00393078 31.67769 9 CGCAGAG 1300 0.0 29.971972 8 GTACATG 620 0.0 29.901499 1 ACGCAGA 1310 0.0 29.743176 7 GTCCTGG 80 4.4511547E-4 29.709822 1 GCAGAGT 1315 0.0 29.630085 9 TCCATAT 65 0.005802881 29.252747 1 >>END_MODULE