FastQCFastQC Report
Thu 26 May 2016
SRR937323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124245
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.7436918990703851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.6712543764336593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.4386494426335064No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5130.4128938790293372No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.190752142943378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.16177713388868767No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC1880.15131393617449396No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1500.12072920439454304No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1290.10382711577930702No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1270.10221739305404645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC200.002069744771.24973
CCAACGA205.3800887E-447.499882-83
GAGTTAG500.001610304637.999847
TAGGCCT1152.4647306E-937.1737564
CTGTTAC550.00256579134.5592231
GTCGCTC300.00394512631.66653360-61
GTAGGCC753.062855E-431.6665333
TAATACT953.4661956E-529.9998744
TTGTAGG804.4604213E-429.6993311
TGTAGGC804.4709624E-429.6873762
GTCCCAG856.359959E-427.952311
TTATATG856.374966E-427.9410572
TAGATTA856.374966E-427.9410575
ACCTGTG700.0083634627.1427427
TCGTGAC350.008379308527.14274276-77
CTAGTCG350.008379308527.14274258-59
TTGAACG350.008379308527.14274274-75
ACCGTTA350.008379308527.14274248-49
ACGCTTT458.927956E-426.38877778-79
GCCTAAA908.902727E-426.3887777
Produced by FastQC (version 0.11.5)