##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937323_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124245 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48146001851181 33.0 31.0 34.0 31.0 34.0 2 32.684824339007605 34.0 31.0 34.0 31.0 34.0 3 32.71840315505654 34.0 31.0 34.0 31.0 34.0 4 36.286071874119685 37.0 35.0 37.0 35.0 37.0 5 36.259591935289144 37.0 35.0 37.0 35.0 37.0 6 36.32687029659141 37.0 37.0 37.0 35.0 37.0 7 36.28049418487665 37.0 37.0 37.0 35.0 37.0 8 36.2600989979476 37.0 37.0 37.0 35.0 37.0 9 37.99839832588837 39.0 38.0 39.0 35.0 39.0 10-11 37.93454062537728 39.0 38.0 39.0 35.0 39.0 12-13 37.85165600225361 39.0 38.0 39.0 35.0 39.0 14-15 39.091834681476115 40.0 38.0 41.0 36.0 41.0 16-17 39.06969696969697 40.0 38.0 41.0 36.0 41.0 18-19 39.105046480743695 40.0 38.5 41.0 36.0 41.0 20-21 39.03571572296672 40.0 38.0 41.0 36.0 41.0 22-23 38.98783854481066 40.0 38.0 41.0 35.0 41.0 24-25 38.99214052879391 40.0 38.0 41.0 35.0 41.0 26-27 38.84274618696929 40.0 38.0 41.0 35.0 41.0 28-29 38.691681757817214 40.0 38.0 41.0 35.0 41.0 30-31 38.699605617932306 40.0 38.0 41.0 35.0 41.0 32-33 38.5364441224999 40.0 38.0 41.0 35.0 41.0 34-35 38.391186768079194 40.0 38.0 41.0 35.0 41.0 36-37 38.223312809368586 40.0 38.0 41.0 34.5 41.0 38-39 38.08270352931707 40.0 38.0 41.0 34.0 41.0 40-41 37.86905308060686 40.0 37.0 41.0 34.0 41.0 42-43 37.62678176184152 40.0 37.0 41.0 33.0 41.0 44-45 37.202446778542395 40.0 37.0 41.0 33.0 41.0 46-47 36.684031550565415 40.0 37.0 41.0 31.5 41.0 48-49 36.21126001046319 40.0 36.0 41.0 30.0 41.0 50-51 35.898470763411 40.0 36.0 41.0 29.0 41.0 52-53 35.65311682562678 39.0 35.5 41.0 28.0 41.0 54-55 35.42807758863536 39.0 35.0 41.0 27.5 41.0 56-57 35.112885830415706 39.0 35.0 40.0 26.5 41.0 58-59 34.8533663326492 38.5 35.0 40.0 26.0 41.0 60-61 34.48568151635881 38.0 34.5 40.0 24.5 41.0 62-63 34.150432612982414 38.0 34.0 40.0 23.5 41.0 64-65 33.75198599541229 37.5 34.0 40.0 22.0 41.0 66-67 33.29849893355869 37.0 33.5 39.5 19.5 41.0 68-69 32.82385609078836 36.0 33.0 39.0 17.0 40.5 70-71 32.363612217795485 36.0 32.5 39.0 11.5 40.0 72-73 31.89896575314902 35.0 32.0 38.0 7.5 40.0 74-75 31.430806068654675 35.0 31.5 37.5 6.0 39.5 76-77 30.138407984224717 34.0 30.0 36.0 2.0 38.5 78-79 30.709597971749368 35.0 31.0 36.0 2.0 39.0 80-81 30.513839591130427 35.0 31.0 36.0 2.0 38.5 82-83 30.129341220974688 34.0 31.0 36.0 2.0 37.0 84-85 29.71435470240251 34.0 30.5 35.0 2.0 37.0 86-87 29.38618455471045 34.0 30.0 35.0 2.0 36.5 88-89 29.008732745784542 34.0 29.5 35.0 2.0 36.0 90-91 28.516004668195905 34.0 29.0 35.0 2.0 35.5 92-93 28.07626463841603 34.0 29.0 35.0 2.0 35.0 94-95 27.62930500221337 33.5 27.5 35.0 2.0 35.0 96-97 27.01620588353656 33.0 26.5 35.0 2.0 35.0 98-99 26.16235260976297 33.0 24.5 35.0 2.0 35.0 100-101 23.37090828604773 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1332123546703059 1101 2 0.04825246456934451 1101 3 0.051393396097267896 1101 4 0.0797640709096541 1101 5 0.24914313534481636 1101 6 0.21025392294990297 1101 7 0.23142658027334306 1101 8 0.24464897470561908 1101 9 0.35782921769196463 1101 10-11 0.22181818512745366 1101 12-13 0.3078307883124509 1101 14-15 0.206403664049283 1101 16-17 0.29018686696321794 1101 18-19 0.3925635060059278 1101 20-21 0.41380638724234586 1101 22-23 0.37321459028450477 1101 24-25 0.5251829337679013 1101 26-27 0.18167192001715193 1101 28-29 -0.2168607636415132 1101 30-31 0.2540283353593722 1101 32-33 0.4182953080525422 1101 34-35 0.1409259968143317 1101 36-37 0.5450833336901297 1101 38-39 0.5499558998552629 1101 40-41 0.6621911533058906 1101 42-43 1.001731410299719 1101 44-45 1.1223990727356679 1101 46-47 0.41074050661243433 1101 48-49 0.5046326385398103 1101 50-51 1.0261921257125834 1101 52-53 1.2395123296685568 1101 54-55 1.1938714828844539 1101 56-57 1.3072036944251124 1101 58-59 1.2194993881066196 1101 60-61 0.9230030719114595 1101 62-63 1.0744528340462551 1101 64-65 1.1442910962387671 1101 66-67 1.2026919250518588 1101 68-69 1.015875320234585 1101 70-71 1.4485214820093937 1101 72-73 1.1165383066217949 1101 74-75 1.1655349590735575 1101 76-77 1.1251191450591165 1101 78-79 1.293962889498026 1101 80-81 0.8226213707673757 1101 82-83 0.9728253513614149 1101 84-85 1.1700596361302935 1101 86-87 0.7446147121416367 1101 88-89 1.182654315620507 1101 90-91 1.4335342348348696 1101 92-93 0.7736390573805743 1101 94-95 0.6455308575381515 1101 96-97 0.5024500175227686 1101 98-99 1.1114277650460487 1101 100-101 0.7089708380061204 1102 1 0.380703993466291 1102 2 0.10313236727533592 1102 3 0.28349281841408214 1102 4 0.24162937951408736 1102 5 0.014041280677744794 1102 6 0.13291278582615718 1102 7 0.18441381043448501 1102 8 0.16031483058886664 1102 9 0.30522964335326463 1102 10-11 0.29687899388391514 1102 12-13 0.1256895599755694 1102 14-15 0.3133890699477391 1102 16-17 0.4796061433390193 1102 18-19 0.4134855187757651 1102 20-21 0.2929299810398547 1102 22-23 0.13607944282326656 1102 24-25 0.5647466309402986 1102 26-27 0.4726228169463127 1102 28-29 0.2682790964983468 1102 30-31 0.5720961371228199 1102 32-33 0.5188007808406212 1102 34-35 0.5511190646160955 1102 36-37 0.4527300348150973 1102 38-39 0.9558733519683642 1102 40-41 0.9161437224758586 1102 42-43 1.0933626051917855 1102 44-45 1.2879699149369372 1102 46-47 1.2439534892819424 1102 48-49 1.4956063387526441 1102 50-51 2.115600000446541 1102 52-53 2.3463837191486476 1102 54-55 2.6043305889957367 1102 56-57 2.4109776378729677 1102 58-59 2.394058524622281 1102 60-61 2.147938462607719 1102 62-63 1.987268279470399 1102 64-65 2.0774394696045277 1102 66-67 2.2356996781683094 1102 68-69 2.402752030480862 1102 70-71 2.515202084016085 1102 72-73 2.814660836266061 1102 74-75 2.6772406446922936 1102 76-77 2.613261437549234 1102 78-79 2.7690693048218264 1102 80-81 2.9149176614332326 1102 82-83 2.543230489701333 1102 84-85 2.893360791496658 1102 86-87 3.058634931053156 1102 88-89 3.3467239416466548 1102 90-91 3.4832453932416776 1102 92-93 4.02105468523099 1102 94-95 3.725437364075095 1102 96-97 4.173037582132071 1102 98-99 5.071940078819232 1102 100-101 6.066488320523604 1103 1 -0.048904134255039367 1103 2 0.1631007008285721 1103 3 -0.05678954073620446 1103 4 -0.033340933192071986 1103 5 0.16485273337599438 1103 6 -0.08189127803451157 1103 7 -0.20653486353305794 1103 8 -0.1512698111844557 1103 9 0.11008516518991485 1103 10-11 -0.13144268443776497 1103 12-13 0.1434029786323876 1103 14-15 -0.144191085745625 1103 16-17 -0.09619541359461437 1103 18-19 0.36687646744972824 1103 20-21 0.16098645286991342 1103 22-23 -0.10366194786151794 1103 24-25 -0.2282009874790205 1103 26-27 0.048622289172200794 1103 28-29 0.12040338730188438 1103 30-31 0.31114482428471746 1103 32-33 -0.23885194379323593 1103 34-35 0.4158439623598582 1103 36-37 0.30472238209046054 1103 38-39 0.360610124629666 1103 40-41 0.6318384051516759 1103 42-43 1.0582326408165343 1103 44-45 0.41480063139029966 1103 46-47 -0.4438021513038919 1103 48-49 -0.784179912731723 1103 50-51 -1.4568369965187529 1103 52-53 -1.0115131010123264 1103 54-55 -0.7415263842197319 1103 56-57 -0.7734464286265705 1103 58-59 -0.6786753452813983 1103 60-61 -0.8754485277604047 1103 62-63 -0.8039310872006666 1103 64-65 -0.6289861802173533 1103 66-67 -0.702814637704499 1103 68-69 -0.6114318987974094 1103 70-71 -0.2892246213540197 1103 72-73 -0.5322516851747601 1103 74-75 -0.1449654510987095 1103 76-77 -0.42533204444047357 1103 78-79 -0.7623332548826163 1103 80-81 -0.12779905581178852 1103 82-83 0.05009155316617253 1103 84-85 -0.053432980768807425 1103 86-87 -0.5975202345359705 1103 88-89 -0.3806147573901555 1103 90-91 -0.3135945592586502 1103 92-93 -0.2823494577957959 1103 94-95 -0.2790487158826842 1103 96-97 -0.20412299314170923 1103 98-99 0.1584950332987134 1103 100-101 1.2490651776287613 1104 1 -0.20852677576447576 1104 2 0.12781768196065002 1104 3 0.029436874358133025 1104 4 -0.12088810300339503 1104 5 0.03523009186655912 1104 6 0.0182974012107735 1104 7 -0.14313863711796415 1104 8 -0.0373075470335138 1104 9 0.17348139160500864 1104 10-11 0.14855731556223617 1104 12-13 0.1385916578776758 1104 14-15 -0.22881372725505855 1104 16-17 -0.0024218286889521323 1104 18-19 0.05093307122331936 1104 20-21 0.12174116985104178 1104 22-23 -0.17885062710680444 1104 24-25 0.3117046728983368 1104 26-27 0.04286757219106363 1104 28-29 0.2531392363584857 1104 30-31 -0.02923253420584615 1104 32-33 -0.5822481702083309 1104 34-35 -0.32255226405523274 1104 36-37 -0.15622101413595857 1104 38-39 -0.1708049697099554 1104 40-41 -0.06476536843323544 1104 42-43 0.01238358421276331 1104 44-45 -0.10227484030782108 1104 46-47 -1.0593681890397377 1104 48-49 -0.9285195353732334 1104 50-51 -0.8867426568961108 1104 52-53 -1.1239659312010062 1104 54-55 -0.9969980823329365 1104 56-57 -0.3548615229661962 1104 58-59 -0.3881093075455553 1104 60-61 -0.4038447541754948 1104 62-63 -0.8640254268233036 1104 64-65 -0.7426654255003626 1104 66-67 -0.5332863358177065 1104 68-69 -0.47445076672193665 1104 70-71 -0.36941330059930166 1104 72-73 -0.6821573455521168 1104 74-75 -0.6596824322307846 1104 76-77 -0.0840112897234917 1104 78-79 0.27700636775889365 1104 80-81 0.29729528381085046 1104 82-83 0.056412307883153545 1104 84-85 -0.10852732039144897 1104 86-87 -0.039407026988801164 1104 88-89 -0.05495438003166342 1104 90-91 0.13527336526965072 1104 92-93 0.819726013902315 1104 94-95 0.5258569444946737 1104 96-97 0.08125436534885466 1104 98-99 0.8970799389590915 1104 100-101 1.893970838006119 1105 1 -0.14939634098186616 1105 2 -0.07131275282196015 1105 3 -0.11708486477230196 1105 4 -0.0561054943077437 1105 5 0.020882265779604836 1105 6 0.0635147925151216 1105 7 0.073817884621171 1105 8 -0.10861189485959954 1105 9 -8.664344819422354E-4 1105 10-11 0.20551383730136052 1105 12-13 0.2562003535298416 1105 14-15 0.11401235970146217 1105 16-17 0.08909991044148313 1105 18-19 0.177889592962444 1105 20-21 0.5741324741988691 1105 22-23 0.044410242458418736 1105 24-25 0.27518293376790126 1105 26-27 0.11373713740845659 1105 28-29 0.22335662766283093 1105 30-31 0.4279413788376374 1105 32-33 0.16014313413949566 1105 34-35 0.7333173011621597 1105 36-37 0.659213768472739 1105 38-39 0.3298472042030909 1105 40-41 0.41219115330589773 1105 42-43 0.43107923638667955 1105 44-45 0.8153338553443561 1105 46-47 1.310197028351567 1105 48-49 2.050828290713717 1105 50-51 1.8495616909299741 1105 52-53 1.929729720972908 1105 54-55 2.0308280046235794 1105 56-57 2.1631819552946823 1105 58-59 2.5129776489761824 1105 60-61 2.28985089799842 1105 62-63 2.3788006601332157 1105 64-65 2.93776935710833 1105 66-67 2.645626707660554 1105 68-69 2.7740274941476244 1105 70-71 2.924064960270261 1105 72-73 2.1763209153174436 1105 74-75 2.1601001764648693 1105 76-77 2.5653365363634606 1105 78-79 2.168962889498026 1105 80-81 2.6351213707673686 1105 82-83 2.6766296991874974 1105 84-85 2.694516157869419 1105 86-87 2.546245146924246 1105 88-89 2.242436924316163 1105 90-91 2.3683168435305184 1105 92-93 3.0290738399892696 1105 94-95 2.6265091184077214 1105 96-97 3.2714717566532023 1105 98-99 4.029906025915615 1105 100-101 4.051362142353948 1106 1 0.03520203779484632 1106 2 -0.0567585892257938 1106 3 -0.18598685445542174 1106 4 -0.08834573012203606 1106 5 -0.14087160304869428 1106 6 0.13812790968534472 1106 7 -0.04279965406711739 1106 8 -0.16646008940639234 1106 9 -0.048213523649224044 1106 10-11 -0.15262912511573035 1106 12-13 -0.41200156246131314 1106 14-15 -0.44991542217031366 1106 16-17 -0.2805574219092932 1106 18-19 -0.27982964064108273 1106 20-21 -0.2555469657421767 1106 22-23 -0.27283367795426017 1106 24-25 0.08128094408477438 1106 26-27 0.25329130100462294 1106 28-29 0.37424093127373936 1106 30-31 0.33297085562465867 1106 32-33 0.13554843996116261 1106 34-35 -0.3283997216823451 1106 36-37 0.49699932484708853 1106 38-39 0.05792384384936611 1106 40-41 0.24896344512608692 1106 42-43 -0.09710794121095745 1106 44-45 -0.8147324674264596 1106 46-47 -0.9004698839549903 1106 48-49 -1.4293669930003574 1106 50-51 -0.7281833348622158 1106 52-53 -0.8463388125569367 1106 54-55 -0.9653031670787016 1106 56-57 -2.080369997542469 1106 58-59 -1.9296347312743691 1106 60-61 -1.7976583134975286 1106 62-63 -2.4308898336029685 1106 64-65 -2.3430891543139225 1106 66-67 -2.557947352766856 1106 68-69 -1.911399919264312 1106 70-71 -1.8100912667009972 1106 72-73 -0.9519031082639842 1106 74-75 -1.433580737315534 1106 76-77 -1.5631638320963717 1106 78-79 -1.2781631237665323 1106 80-81 -1.0982979365281302 1106 82-83 -0.8419775226253208 1106 84-85 -1.2015781678490747 1106 86-87 -1.3909324507176173 1106 88-89 -0.7969882783367517 1106 90-91 -0.9684554482896708 1106 92-93 -2.429935003046836 1106 94-95 -2.287363394488377 1106 96-97 -1.9589998719392838 1106 98-99 -3.3645302305324343 1106 100-101 -1.687215602671845 1107 1 -0.37482814562748956 1107 2 -0.2123193043407241 1107 3 -0.441385043450083 1107 4 -0.19979221259243474 1107 5 -0.5172356615580966 1107 6 -0.27909985906320145 1107 7 -0.19464548643303203 1107 8 -0.25840343744447125 1107 9 -0.3026829919566296 1107 10-11 -0.7444563830679058 1107 12-13 -0.2079151914374009 1107 14-15 -0.21669043958382872 1107 16-17 -0.6431067601957992 1107 18-19 0.1714125232781143 1107 20-21 0.05023432053597077 1107 22-23 -0.33111090107940555 1107 24-25 -0.5782268339509784 1107 26-27 -0.09405023602811013 1107 28-29 -0.09871007871000614 1107 30-31 -0.8663558218770788 1107 32-33 -1.2678646085645013 1107 34-35 -1.8323467846031747 1107 36-37 -0.9386867675606183 1107 38-39 -1.4548460656003641 1107 40-41 -0.9281900259674813 1107 42-43 -1.7906301144173753 1107 44-45 -1.3919323745543934 1107 46-47 -1.0097106547931602 1107 48-49 -0.8785195353732362 1107 50-51 -1.1581810130604921 1107 52-53 -1.2491714106530623 1107 54-55 -1.69028575356581 1107 56-57 -1.2224642626922204 1107 58-59 -0.9586572527510313 1107 60-61 -0.9181598226686418 1107 62-63 -1.0272446049054977 1107 64-65 -0.7628709049524218 1107 66-67 -0.1549986645848307 1107 68-69 -0.7711630954890651 1107 70-71 -0.636536588270534 1107 72-73 -0.558938167469929 1107 74-75 -0.4613262678472232 1107 76-77 -0.5523674541070562 1107 78-79 -0.3597744541589165 1107 80-81 -0.4079101956412039 1107 82-83 -0.2892726236236989 1107 84-85 0.24037678919759387 1107 86-87 -0.0021467530161949355 1107 88-89 0.03874425010532079 1107 90-91 0.04431446116005944 1107 92-93 0.014109575546150666 1107 94-95 -0.18044442536833927 1107 96-97 0.24392559822556592 1107 98-99 0.4115319937536128 1107 100-101 0.9469845366362577 1108 1 -0.14408233132002835 1108 2 -0.6266267624837951 1108 3 -0.10500757008630757 1108 4 -0.1964436585589482 1108 5 -0.6636587970223289 1108 6 -0.3850359321225625 1108 7 -0.08536085934018445 1108 8 0.29380356407759933 1108 9 0.0312591693827855 1108 10-11 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0.15430216118131312 2316 100-101 0.36637824541352515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 10.0 7 11.0 8 29.0 9 42.0 10 88.0 11 196.0 12 287.0 13 385.0 14 538.0 15 796.0 16 1275.0 17 2033.0 18 1816.0 19 978.0 20 724.0 21 756.0 22 707.0 23 728.0 24 812.0 25 899.0 26 1076.0 27 1152.0 28 1348.0 29 1612.0 30 2059.0 31 2614.0 32 3518.0 33 4841.0 34 7101.0 35 10910.0 36 18015.0 37 29412.0 38 25070.0 39 2407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99495901177304 21.069881303248458 16.130357056578248 19.80480262840025 2 15.552336110105033 22.20532013360699 40.78313010583927 21.45921365044871 3 19.137188619260332 26.9121493822689 31.682562678578613 22.268099319892148 4 12.240331602881403 19.065555957986238 38.815244074208216 29.878868364924145 5 12.17674755523361 39.59515473459697 34.5309670409272 13.697130669242222 6 28.950863213811424 39.39313453257676 17.61922008933961 14.036782164272205 7 26.318161696647753 35.43241176707313 21.42379975049298 16.82562678578615 8 26.046118556078717 35.813111191597244 19.484888727916616 18.655881524407423 9 26.828443800555352 17.463076984989335 20.964223912431084 34.74425530202422 10-11 24.302386413940198 28.57620024950702 27.241740110266004 19.879673226286773 12-13 25.91184034191749 26.30140735106507 27.975177176524564 19.811575130492876 14-15 22.372731297034086 27.476759628154053 27.454223510000404 22.69628556481146 16-17 20.207654231558614 30.753752666103264 28.231317155619944 20.80727594671818 18-19 20.047889251076505 28.429313050826995 32.170308664332566 19.352489033763934 20-21 21.210596847345357 27.094961950025958 32.39419048730527 19.300250715323415 22-23 20.822014833774 27.403043217552632 32.195648062071655 19.579293886601715 24-25 20.834641233047606 27.446979757736727 32.409352489033765 19.3090265201819 26-27 20.457572891723846 27.74493430186933 32.41483389339397 19.382658913012857 28-29 19.787348781803107 28.449948084770725 32.464323371511824 19.298379761914344 30-31 20.175138537319558 28.00848327290142 32.068220323636055 19.748157866142968 32-33 20.19646744926335 28.363830994530947 31.67262937192391 19.7670721842818 34-35 20.4358324278643 28.314217875970865 32.00651937703731 19.24343031912753 36-37 20.971870095376072 27.75041249144835 31.878143989697776 19.399573423477808 38-39 20.35212684615075 28.35848525091553 32.039116262223835 19.250271640709887 40-41 20.325163990502638 28.714636403879435 31.691013722886236 19.269185882731698 42-43 20.18481178738595 28.8619505853849 31.08717787732071 19.866059749908437 44-45 20.737253008169343 28.489275222342954 30.894603404563565 19.87886836492414 46-47 20.792386011509517 29.228137953237553 30.342066079117874 19.637409956135055 48-49 20.886152360255945 29.525131796048132 29.67926274699183 19.909453096704095 50-51 20.767032878586665 29.53197311763049 29.6172884220693 20.08370558171355 52-53 20.98676003058473 30.03863334540625 29.074811863656485 19.899794760352528 54-55 20.457161253974 29.79838222866111 29.222101493017828 20.522355024347057 56-57 20.95818745221136 29.57141132439937 29.5376071471689 19.932794076220368 58-59 20.948931546541107 29.025715320536037 29.328343192885026 20.697009940037827 60-61 20.449515071029015 29.392732101895447 30.084912873757496 20.07283995331804 62-63 20.476075495995815 29.01726427622842 30.323151837096063 20.183508390679705 64-65 20.156947965712906 29.17783411807316 30.175862207734717 20.489355708479216 66-67 20.092961487383796 28.888888888888886 29.713871785584935 21.30427783814238 68-69 20.096180932834322 28.772988852670128 29.91347740351725 21.21735281097831 70-71 20.35655358364522 29.13034729767798 29.69898185037627 20.814117268300535 72-73 20.256750774679062 28.222866111312328 30.027365286329427 21.493017827679182 74-75 19.994768401142903 28.715038834560747 29.322709163346612 21.967483600949738 76-77 19.828162099078433 27.9882490241056 29.93037949213248 22.25320938468349 78-79 19.86760030584732 27.663889894965592 29.715481508310194 22.753028290876898 80-81 19.65431204475029 28.206366453378408 29.78590687754034 22.35341462433096 82-83 19.81608917863898 28.38906998269548 29.476437683608996 22.31840315505654 84-85 19.48690088132319 28.909010422954644 28.739587106120972 22.86450158960119 86-87 19.03899553301944 28.47116584168377 28.560103022254417 23.929735603042378 88-89 19.098957704535394 28.044186888808404 28.94120487745986 23.91565052919635 90-91 19.450492399698966 27.793719338208366 29.02159958466981 23.73418867742286 92-93 19.434741315475996 27.57356492064003 28.556710988055762 24.43498277582821 94-95 19.112640347700108 28.51905509276027 28.254658135136225 24.113646424403395 96-97 19.68932351402471 29.037385810294175 27.662682602921645 23.61060807275947 98-99 19.31989214857741 29.90381906716568 25.987766107288017 24.788522676968892 100-101 19.47631715572441 29.704875015595384 25.120436590185573 25.698371238494634 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 77.0 1 52.5 2 36.0 3 38.0 4 36.0 5 41.5 6 52.0 7 104.0 8 169.5 9 214.0 10 229.0 11 223.0 12 244.5 13 279.0 14 300.0 15 325.0 16 343.0 17 358.0 18 376.5 19 400.5 20 444.0 21 475.0 22 520.0 23 615.5 24 717.0 25 835.5 26 1027.5 27 1294.5 28 1464.5 29 1698.5 30 2095.0 31 2444.5 32 2737.0 33 2960.0 34 3257.5 35 3505.0 36 3804.5 37 4123.5 38 4240.5 39 4293.5 40 4328.5 41 4429.0 42 4456.0 43 4451.0 44 4583.0 45 4682.0 46 4554.5 47 4686.0 48 5127.5 49 4899.0 50 4252.5 51 3804.0 52 3397.0 53 3148.5 54 2987.0 55 2679.0 56 2268.5 57 1930.0 58 1715.0 59 1468.5 60 1100.0 61 819.0 62 632.5 63 438.0 64 298.5 65 202.0 66 128.0 67 95.0 68 79.5 69 47.5 70 41.5 71 31.5 72 16.5 73 16.5 74 13.5 75 8.0 76 6.5 77 6.0 78 3.0 79 0.0 80 0.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05070626584570808 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003621876131836291 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.0243068131514347E-4 22-23 0.0012072920439454304 24-25 0.0 26-27 0.0020121534065757176 28-29 0.0032194454505211478 30-31 4.0243068131514347E-4 32-33 4.0243068131514347E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008451044307618014 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.005231598857096865 92-93 8.048613626302869E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.007646182944987726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 124245.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.66135458167331 #Duplication Level Percentage of deduplicated Percentage of total 1 89.5281523883445 66.8429313050827 2 6.258961008160581 9.346050142862893 3 1.6978752304259244 3.802969938428106 4 0.7502991494453608 2.2407340335627186 5 0.4387525198624452 1.6378928729526339 6 0.2608798766749674 1.1686586985391767 7 0.18973081939997627 0.9915891987605134 8 0.15954637085907097 0.9529558533542598 9 0.1099576339704408 0.7388627308946034 >10 0.5616463460647025 7.417602318000724 >50 0.03018444854090532 1.4873837981407703 >100 0.009702144173862424 1.1058795122540144 >500 0.004312064077272189 2.2664895971668884 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 924 0.7436918990703851 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.6712543764336593 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.4386494426335064 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 513 0.4128938790293372 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 237 0.190752142943378 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.16177713388868767 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 188 0.15131393617449396 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 150 0.12072920439454304 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 129 0.10382711577930702 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 127 0.10221739305404645 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.0243068131514347E-4 0.0 24-25 0.0 0.0 0.0 0.0032194454505211478 0.0 26-27 0.0 0.0 0.0 0.004426737494466578 0.0 28-29 0.0 0.0 0.0 0.007646182944987725 0.0 30-31 0.0 0.0 0.0 0.014487504527345164 8.048613626302869E-4 32-33 0.0 0.0 0.0 0.021328826109702603 8.048613626302869E-4 34-35 0.0 0.0 0.0 0.02656042496679947 8.048613626302869E-4 36-37 0.0 0.0 0.0 0.035413899955732624 8.048613626302869E-4 38-39 0.0 0.0 0.0 0.053523280614914084 8.048613626302869E-4 40-41 0.0 0.0 0.0 0.06680349309831382 8.048613626302869E-4 42-43 0.0 0.0 0.0 0.11509517485613104 8.048613626302869E-4 44-45 0.0 0.0 0.0 0.18471568272365085 8.048613626302869E-4 46-47 0.0 0.0 0.0 0.2543361905911707 8.048613626302869E-4 48-49 0.0 0.0 0.0 0.41329630971065234 8.048613626302869E-4 50-51 0.0 0.0 0.0 0.5485130186325405 8.048613626302869E-4 52-53 0.0 0.0 0.0 0.7167290434222705 8.048613626302869E-4 54-55 0.0 0.0 0.0 1.0559781077709365 8.048613626302869E-4 56-57 0.0 0.0 0.0 1.4020684937019599 8.048613626302869E-4 58-59 0.0 0.0 0.0 1.8713026681154172 8.048613626302869E-4 60-61 0.0 0.0 0.0 2.3771580345285526 8.048613626302869E-4 62-63 0.0 0.0 0.0 3.1264839631373498 8.048613626302869E-4 64-65 0.0 0.0 0.0 3.6790212885830416 8.048613626302869E-4 66-67 0.0 0.0 0.0 4.4005795001810935 8.048613626302869E-4 68-69 0.0 0.0 0.0 5.240854762767113 8.048613626302869E-4 70-71 0.0 0.0 0.0 5.967242142540947 8.048613626302869E-4 72-73 0.0 0.0 0.0 7.138315425168015 8.048613626302869E-4 74-75 0.0 0.0 0.0 8.417642561068856 8.048613626302869E-4 76-77 0.0 0.0 0.0 9.597166888003542 8.048613626302869E-4 78-79 0.0 0.0 0.0 10.60002414584088 8.048613626302869E-4 80-81 0.0 0.0 0.0 11.875729405609883 8.048613626302869E-4 82-83 0.0 0.0 0.0 13.089460340456355 8.048613626302869E-4 84-85 0.0 0.0 0.0 14.324520101412531 8.048613626302869E-4 86-87 0.0 0.0 0.0 16.32258843414222 8.048613626302869E-4 88-89 0.0 0.0 0.0 18.914242021811745 8.048613626302869E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTACGC 20 0.0020697447 71.2497 3 CCAACGA 20 5.3800887E-4 47.4998 82-83 GAGTTAG 50 0.0016103046 37.99984 7 TAGGCCT 115 2.4647306E-9 37.173756 4 CTGTTAC 55 0.002565791 34.559223 1 GTCGCTC 30 0.003945126 31.666533 60-61 GTAGGCC 75 3.062855E-4 31.666533 3 TAATACT 95 3.4661956E-5 29.999874 4 TTGTAGG 80 4.4604213E-4 29.699331 1 TGTAGGC 80 4.4709624E-4 29.687376 2 GTCCCAG 85 6.359959E-4 27.95231 1 TTATATG 85 6.374966E-4 27.941057 2 TAGATTA 85 6.374966E-4 27.941057 5 ACCTGTG 70 0.00836346 27.142742 7 TCGTGAC 35 0.0083793085 27.142742 76-77 CTAGTCG 35 0.0083793085 27.142742 58-59 TTGAACG 35 0.0083793085 27.142742 74-75 ACCGTTA 35 0.0083793085 27.142742 48-49 ACGCTTT 45 8.927956E-4 26.388777 78-79 GCCTAAA 90 8.902727E-4 26.388777 7 >>END_MODULE