FastQCFastQC Report
Thu 26 May 2016
SRR937322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117200
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.8959044368600683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.7372013651877133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.4453924914675768No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2120.18088737201365188No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.17832764505119453No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1790.15273037542662116No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1560.13310580204778155No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1420.12116040955631399No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1210.10324232081911262No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1190.10153583617747441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAT452.0300831E-763.3404545
TTAGGCA405.365264E-447.505344
TTAGGGC500.001609000438.0042723
GTATAGC651.3167129E-436.5581931
ATGGGAT1750.035.2896845
GCATTCC550.002568788434.549348
CAGTGCA702.0407706E-433.932399
GGTATCA8300.033.2106931
ATGGGGA856.3683867E-427.9443195
ACAGTGC700.00835675127.145918
CTACACT700.00835675127.145914
GTACATG5300.026.9013121
TACATGG5550.026.5345152
GTATCAA11500.024.7959941
GAGATGG1351.3282108E-524.63249
GAGTACT7650.024.21840912-13
CATGGGA3700.023.1107084
ACATGGG5400.022.8729443
ATTAGGG1050.002183108622.6215922
CAGAGTG1050.002183108622.6215924