##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937322_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117200 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04836177474403 33.0 31.0 34.0 31.0 34.0 2 32.165912969283276 33.0 31.0 34.0 31.0 34.0 3 32.17669795221843 34.0 31.0 34.0 30.0 34.0 4 35.77243174061434 37.0 35.0 37.0 35.0 37.0 5 35.7869795221843 37.0 35.0 37.0 35.0 37.0 6 35.84985494880546 37.0 36.0 37.0 35.0 37.0 7 35.8091638225256 37.0 36.0 37.0 35.0 37.0 8 35.80264505119454 37.0 36.0 37.0 35.0 37.0 9 37.47519624573379 39.0 37.0 39.0 35.0 39.0 10-11 37.394739761092154 39.0 37.0 39.0 35.0 39.0 12-13 37.26982508532423 39.0 37.0 39.0 35.0 39.0 14-15 38.47240187713311 40.0 38.0 41.0 34.0 41.0 16-17 38.46266638225256 40.0 38.0 41.0 34.5 41.0 18-19 38.49804607508533 40.0 38.0 41.0 34.5 41.0 20-21 38.48175767918089 40.0 38.0 41.0 34.0 41.0 22-23 38.337470136518775 40.0 38.0 41.0 34.0 41.0 24-25 38.188383105802046 40.0 38.0 41.0 34.0 41.0 26-27 38.037952218430036 40.0 38.0 41.0 34.0 41.0 28-29 38.03946245733788 40.0 38.0 41.0 34.0 41.0 30-31 37.82542662116041 40.0 37.5 41.0 33.5 41.0 32-33 37.55074232081911 40.0 37.0 41.0 33.0 41.0 34-35 37.392218430034134 40.0 36.5 41.0 33.0 41.0 36-37 37.36582337883959 40.0 37.0 41.0 33.0 41.0 38-39 37.22904863481229 40.0 37.0 41.0 33.0 41.0 40-41 37.033698805460745 40.0 36.5 41.0 32.5 41.0 42-43 36.706996587030716 40.0 36.0 41.0 31.0 41.0 44-45 36.35766638225256 40.0 36.0 41.0 30.5 41.0 46-47 35.8255204778157 40.0 35.5 41.0 28.0 41.0 48-49 35.356651023890784 39.0 35.0 41.0 25.0 41.0 50-51 34.437197098976114 38.5 34.0 40.0 23.5 40.5 52-53 34.476796075085325 38.5 35.0 40.0 23.0 40.5 54-55 34.75174488054607 39.0 35.0 40.5 22.5 41.0 56-57 34.565810580204776 39.0 35.0 41.0 20.5 41.0 58-59 34.3356271331058 39.0 35.0 40.5 18.5 41.0 60-61 34.03389078498294 38.5 34.5 40.0 14.0 41.0 62-63 33.72054180887372 38.0 34.0 40.0 8.5 41.0 64-65 33.337627986348124 38.0 34.0 40.0 4.5 41.0 66-67 32.93400597269625 37.0 33.5 40.0 2.0 41.0 68-69 32.48972696245734 37.0 33.0 39.5 2.0 41.0 70-71 32.03710324232082 36.0 32.5 39.0 2.0 41.0 72-73 31.632367747440274 36.0 32.0 39.0 2.0 40.0 74-75 31.195883105802046 35.0 31.5 38.5 2.0 40.0 76-77 30.755153583617748 35.0 31.0 37.5 2.0 39.5 78-79 30.311693686006826 35.0 31.0 37.0 2.0 39.0 80-81 29.85233361774744 35.0 30.5 36.5 2.0 39.0 82-83 29.369846416382252 34.5 30.0 36.0 2.0 37.5 84-85 28.95683447098976 34.0 29.0 35.5 2.0 37.0 86-87 28.53168941979522 34.0 29.0 35.0 2.0 37.0 88-89 28.135793515358362 34.0 29.0 35.0 2.0 36.0 90-91 27.750959897610922 34.0 27.5 35.0 2.0 36.0 92-93 27.34329351535836 34.0 26.5 35.0 2.0 35.5 94-95 26.857052047781572 34.0 25.5 35.0 2.0 35.0 96-97 26.309215017064844 33.0 25.0 35.0 2.0 35.0 98-99 25.614961604095562 33.0 22.0 35.0 2.0 35.0 100-101 23.85663395904437 31.0 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19399617776187128 1101 2 0.2474709166404736 1101 3 0.3211837364231158 1101 4 0.37694816046332136 1101 5 0.45016386566688027 1101 6 0.5519194001026619 1101 7 0.4068681277883144 1101 8 0.3076776323323145 1101 9 0.5496391801761504 1101 10-11 0.3898622623589105 1101 12-13 0.1918132945763773 1101 14-15 0.28270416260607334 1101 16-17 0.3743536052640053 1101 18-19 0.7902715506326885 1101 20-21 0.6549653076955195 1101 22-23 0.922452821553243 1101 24-25 0.6139111322998829 1101 26-27 0.6499310949600883 1101 28-29 0.667751241791315 1101 30-31 0.30165019195717235 1101 32-33 0.5257701976836557 1101 34-35 0.7851512099942397 1101 36-37 0.5992162490374753 1101 38-39 0.871615828295667 1101 40-41 1.003263612195319 1101 42-43 1.162532090611812 1101 44-45 1.214520849243641 1101 46-47 1.7986642912818525 1101 48-49 1.7609740602883122 1101 50-51 1.7905734987811996 1101 52-53 1.960926876909646 1101 54-55 1.9227937609559334 1101 56-57 2.0347755559692615 1101 58-59 2.2841307015666032 1101 60-61 2.331070736930471 1101 62-63 2.1086150731293145 1101 64-65 2.2884602062029984 1101 66-67 2.71933423566524 1101 68-69 2.8273744284621216 1101 70-71 2.968098831376217 1101 72-73 2.6239484581189068 1101 74-75 2.6385935230984394 1101 76-77 2.557572571054621 1101 78-79 2.4662108796605473 1101 80-81 2.588582642294732 1101 82-83 2.8233336533483744 1101 84-85 2.9790361639290346 1101 86-87 2.871367532845756 1101 88-89 2.7347906855766553 1101 90-91 2.732596660303898 1101 92-93 2.2655707317651945 1101 94-95 2.550608288854427 1101 96-97 2.2748418129189965 1101 98-99 2.230176319753266 1101 100-101 2.794073011738387 1102 1 0.2304231870565694 1102 2 0.18958409323970216 1102 3 0.20926009873402762 1102 4 0.048467205478807784 1102 5 0.271081489140542 1102 6 0.1049994073926186 1102 7 0.22375349319746363 1102 8 -0.36548165325179127 1102 9 -0.20427917229332593 1102 10-11 0.17931916048174656 1102 12-13 -0.060123100539350105 1102 14-15 0.2997216585054687 1102 16-17 0.3813587993591412 1102 18-19 0.3081547290544151 1102 20-21 0.15820022659473665 1102 22-23 0.5197965430768434 1102 24-25 0.41393846964269443 1102 26-27 0.4627044683881891 1102 28-29 0.6488201318951994 1102 30-31 0.5473901227025735 1102 32-33 0.733499831363261 1102 34-35 0.5958628921520699 1102 36-37 0.42176272252024916 1102 38-39 0.28985439217392184 1102 40-41 0.226905857502409 1102 42-43 0.3198129362469473 1102 44-45 0.6495718789502121 1102 46-47 0.8377794726195589 1102 48-49 0.8710410367781947 1102 50-51 0.8845456146915325 1102 52-53 0.7649295195194483 1102 54-55 1.016731705187759 1102 56-57 0.8413410744675289 1102 58-59 0.840058348732633 1102 60-61 1.058619944147523 1102 62-63 1.0815169320686309 1102 64-65 1.4254089031229924 1102 66-67 1.0689674596492047 1102 68-69 1.1476769617225528 1102 70-71 1.585512718746358 1102 72-73 1.7185812264804845 1102 74-75 2.0193913178861145 1102 76-77 1.5707628389605794 1102 78-79 1.5560254413518173 1102 80-81 1.3108808015803142 1102 82-83 1.415916120999185 1102 84-85 1.6593828925603447 1102 86-87 1.5081795430516998 1102 88-89 1.572110714736489 1102 90-91 1.4130459039707475 1102 92-93 2.0131103709122655 1102 94-95 2.0094314162464464 1102 96-97 1.976329419990261 1102 98-99 2.3592199683772854 1102 100-101 2.260630329033816 1103 1 -0.45630971767750594 1103 2 -0.3204100622249335 1103 3 -0.4123257185268301 1103 4 -0.24204516545437116 1103 5 -0.15642679061787845 1103 6 -0.19007170112092098 1103 7 -0.7485823727678564 1103 8 -0.7307261496476585 1103 9 -0.6420738342843677 1103 10-11 -0.564475891144987 1103 12-13 -0.5072690191054932 1103 14-15 -0.03645601536945264 1103 16-17 -0.47404739584833777 1103 18-19 -0.7531934104351663 1103 20-21 -0.9703405877438556 1103 22-23 -0.6312457323955911 1103 24-25 -0.9027184561317085 1103 26-27 -0.9935199617696142 1103 28-29 -0.6766035969183619 1103 30-31 -0.801158484349834 1103 32-33 -0.6340045520382489 1103 34-35 -1.1004160870024222 1103 36-37 -0.6358643961238215 1103 38-39 0.05679380382403565 1103 40-41 0.6174182284355894 1103 42-43 0.429495383159086 1103 44-45 0.5656053876196125 1103 46-47 0.25909254489697275 1103 48-49 0.25005915483780683 1103 50-51 -0.04432638998409999 1103 52-53 -0.07420938560425583 1103 54-55 0.4336808577301241 1103 56-57 0.6483739986833896 1103 58-59 0.8759827594086502 1103 60-61 1.3044995467191214 1103 62-63 0.8659704680125202 1103 64-65 0.719369549918234 1103 66-67 0.7996317885017241 1103 68-69 1.2725357187846953 1103 70-71 1.7543613452805573 1103 72-73 1.5705001822568398 1103 74-75 0.9715050915077796 1103 76-77 0.9113823597086821 1103 78-79 0.8480379096939181 1103 80-81 0.7232322529732613 1103 82-83 1.0619320960625025 1103 84-85 0.6742669759423805 1103 86-87 0.564735163546537 1103 88-89 0.5297573151650887 1103 90-91 0.5732251363884373 1103 92-93 0.8570612768152479 1103 94-95 0.40668448462750817 1103 96-97 -0.29588955526787686 1103 98-99 -0.0342146702356132 1103 100-101 -0.2600982897076598 1104 1 0.35197643775224563 1104 2 0.1707376475022926 1104 3 0.16621262424208538 1104 4 0.36485138626976976 1104 5 0.3929298743332268 1104 6 0.40519335291922687 1104 7 0.29336307242000004 1104 8 0.04606232178826275 1104 9 0.5649256510475951 1104 10-11 0.5997804135384897 1104 12-13 0.3074247534112331 1104 14-15 0.3808625641467529 1104 16-17 0.46357483781094544 1104 18-19 0.6777896055195427 1104 20-21 0.1543634781336536 1104 22-23 0.3471639433635474 1104 24-25 0.6039207615728728 1104 26-27 0.3102885817198384 1104 28-29 0.7689187911413384 1104 30-31 0.7179356999013251 1104 32-33 0.6114406358155762 1104 34-35 0.46636209107272464 1104 36-37 0.6171206785030492 1104 38-39 0.8904530935821384 1104 40-41 1.0635067513383163 1104 42-43 1.119922557631547 1104 44-45 0.641518923389043 1104 46-47 1.7640590914744365 1104 48-49 1.082020040066844 1104 50-51 1.045689050057078 1104 52-53 0.7792646236597633 1104 54-55 1.1568151860883376 1104 56-57 1.1814414201965135 1104 58-59 0.4338057136031921 1104 60-61 0.7744686666367784 1104 62-63 0.8623163249348096 1104 64-65 1.2358903939738255 1104 66-67 0.4379488240138194 1104 68-69 0.14044616654589248 1104 70-71 0.25348640961405877 1104 72-73 0.4746947267756241 1104 74-75 0.1584839901181745 1104 76-77 0.591696307212537 1104 78-79 1.0329838695498097 1104 80-81 0.6499692576052745 1104 82-83 0.3972383235457748 1104 84-85 -0.07876956548839331 1104 86-87 0.0702421115650651 1104 88-89 0.38855813863395383 1104 90-91 0.473147936182567 1104 92-93 0.10873394794237257 1104 94-95 0.14338031581639044 1104 96-97 0.20259217401673268 1104 98-99 0.7033046298158538 1104 100-101 1.3079150916613536 1105 1 -0.3706472857355827 1105 2 -0.3044391003374578 1105 3 -0.6685870615395331 1105 4 -0.6772538768881233 1105 5 -0.45624530241461514 1105 6 -0.482630684786983 1105 7 -0.8298890878313756 1105 8 -0.542704371063266 1105 9 -0.33499579435695637 1105 10-11 -0.8156555644662191 1105 12-13 -0.5853089465102173 1105 14-15 -0.6861837830645499 1105 16-17 -0.6945555628174915 1105 18-19 -0.4459338823044945 1105 20-21 -0.7741518400124576 1105 22-23 -0.5653655146097449 1105 24-25 -0.8936440459683652 1105 26-27 -0.7499627929855919 1105 28-29 -0.5660772811288908 1105 30-31 -0.5122292647491022 1105 32-33 -1.0828248787170196 1105 34-35 -1.4392545625015174 1105 36-37 -1.4042854487553953 1105 38-39 -1.7733332379182514 1105 40-41 -1.8368467443411802 1105 42-43 -2.0979093355342044 1105 44-45 -2.1242312729974486 1105 46-47 -2.128203280874345 1105 48-49 -1.5067466527846918 1105 50-51 -2.1671939035957166 1105 52-53 -2.1299262640071603 1105 54-55 -2.5189507212172444 1105 56-57 -3.415691337069788 1105 58-59 -3.689171505564129 1105 60-61 -3.14450226816291 1105 62-63 -2.9588934158350284 1105 64-65 -2.738343698720602 1105 66-67 -2.476230280463792 1105 68-69 -2.3011484917416176 1105 70-71 -2.223497093920898 1105 72-73 -2.5786831208284617 1105 74-75 -2.727768955679153 1105 76-77 -2.164116732850303 1105 78-79 -1.9298205295075341 1105 80-81 -2.0493630283334525 1105 82-83 -2.544238502848568 1105 84-85 -2.7547711365803025 1105 86-87 -2.3071341649471115 1105 88-89 -2.3099886013503372 1105 90-91 -1.654542558711384 1105 92-93 -2.0360421714606147 1105 94-95 -1.5365097077499854 1105 96-97 -0.9367244010029019 1105 98-99 -0.6706937990922377 1105 100-101 -0.4967589067675533 1106 1 -0.3074893909987395 1106 2 -0.557070679284827 1106 3 0.08667609635520535 1106 4 0.11485138626976976 1106 5 -0.37729793399356026 1106 6 -0.029999105839614515 1106 7 -0.08252066677874836 1106 8 -0.3190201605369509 1106 9 0.10184631090619689 1106 10-11 -0.26039240657148355 1106 12-13 -0.19188789387863636 1106 14-15 -0.14407851990665677 1106 16-17 0.043602331919352366 1106 18-19 0.14617138085340287 1106 20-21 0.23900605472438485 1106 22-23 0.010950274863937182 1106 24-25 -0.24495983544205302 1106 26-27 -0.19206805614347644 1106 28-29 0.1431332451868954 1106 30-31 0.13250757735615792 1106 32-33 -0.08808803661175801 1106 34-35 0.37258754276164296 1106 36-37 0.04308297229723479 1106 38-39 0.06877202523963888 1106 40-41 0.6671006240798718 1106 42-43 0.8836696118342147 1106 44-45 0.870505569107813 1106 46-47 1.3941651401782806 1106 48-49 0.9143059787942533 1106 50-51 0.6617534648253383 1106 52-53 0.6963895254665218 1106 54-55 0.8757861208880229 1106 56-57 1.5027297155617916 1106 58-59 2.180565336541129 1106 60-61 1.404181942363408 1106 62-63 1.538475005217606 1106 64-65 1.2142878802267703 1106 66-67 1.977717087957263 1106 68-69 1.525167297732068 1106 70-71 1.5317660639738406 1106 72-73 1.5252642475925917 1106 74-75 0.35644157063663684 1106 76-77 -0.035169364429251004 1106 78-79 0.8570215757556205 1106 80-81 1.21379486640339 1106 82-83 0.7583930760987982 1106 84-85 1.5097025476302228 1106 86-87 1.2757605718949954 1106 88-89 1.1373798197022964 1106 90-91 1.807299546551775 1106 92-93 1.195536775907808 1106 94-95 1.0819113448815934 1106 96-97 1.59880191478657 1106 98-99 1.621411464065659 1106 100-101 1.3085042511271858 1107 1 0.04530666163283925 1107 2 -0.29637988981114205 1107 3 -0.09917916680269201 1107 4 -0.38514861373023024 1107 5 -0.0261466182040877 1107 6 -0.5110846321554021 1107 7 -0.07347461414716605 1107 8 -0.5229675289580058 1107 9 -0.4080221101464332 1107 10-11 0.11214048816535893 1107 12-13 -0.7058681570365266 1107 14-15 -0.4816607567487665 1107 16-17 -0.9613318786069627 1107 18-19 -0.3826115138834396 1107 20-21 -1.1187242084335125 1107 22-23 -1.194230646188693 1107 24-25 -1.080897335442053 1107 26-27 -1.3919035824592711 1107 28-29 -1.1891035969183648 1107 30-31 -1.162311501591212 1107 32-33 -1.1012459313486005 1107 34-35 -0.31162298355414464 1107 36-37 -0.8093018961238201 1107 38-39 -0.9900273168656213 1107 40-41 0.19177167671144701 1107 42-43 0.2603143486763244 1107 44-45 0.43629504279202536 1107 46-47 0.24120461386249303 1107 48-49 0.5833026893205684 1107 50-51 0.48412188587796834 1107 52-53 -0.1983473166387384 1107 54-55 0.07274335773012552 1107 56-57 0.5829106366144217 1107 58-59 1.0913383628569235 1107 60-61 1.0069779949949833 1107 62-63 1.151961847322866 1107 64-65 0.4605872223320304 1107 66-67 1.008144719536208 1107 68-69 0.6912857187846981 1107 70-71 1.2344798797633132 1107 72-73 1.3739484581189068 1107 74-75 1.1426257811629519 1107 76-77 0.378893135570749 1107 78-79 0.6234689441766754 1107 80-81 0.34866328745602004 1107 82-83 0.5980312339935345 1107 84-85 0.46693938973548654 1107 86-87 0.9784743876844679 1107 88-89 0.592149556544399 1107 90-91 0.03098375707808998 1107 92-93 -0.8422921714606133 1107 94-95 -0.19687154985524913 1107 96-97 0.045759151628676165 1107 98-99 -0.553341825408026 1107 100-101 0.534244382706131 1108 1 -0.6513971054904495 1108 2 -0.948563108989223 1108 3 -0.9369360161177589 1108 4 -0.7721349151000965 1108 5 -0.9681414812177849 1108 6 -1.0429767554430711 1108 7 -1.0017708470238773 1108 8 -1.0452278029306115 1108 9 -0.6057960827491726 1108 10-11 -0.260933141971627 1108 12-13 -0.07680137621461114 1108 14-15 0.19117742818274053 1108 16-17 -0.20234215257956834 1108 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5 0.599895048462578 2311 6 0.33023481228904217 2311 7 0.597011496963944 2311 8 0.5742546932642156 2311 9 0.49892233429800825 2311 10-11 -0.16945673405685824 2311 12-13 0.22989573185235912 2311 14-15 0.2980267432512278 2311 16-17 0.5615847880597045 2311 18-19 0.16634681944989183 2311 20-21 0.23578968045537607 2311 22-23 -0.15308481285536146 2311 24-25 -0.308848724330943 2311 26-27 0.301499195318506 2311 28-29 0.056729736414972365 2311 30-31 -0.7812351127023263 2311 32-33 -0.5956903757930476 2311 34-35 -1.1866229835541446 2311 36-37 -3.03586439612382 2311 38-39 -2.970409261310067 2311 40-41 -3.467256101066333 2311 42-43 -3.0973245402125613 2311 44-45 -1.9914827349857518 2311 46-47 -2.8872676083597284 2311 48-49 -1.9342320329016545 2311 50-51 -1.3297670030109217 2311 52-53 -2.0094584277498484 2311 54-55 -1.6329858089365459 2311 56-57 -1.6665685300522455 2311 58-59 -1.6880019149208536 2311 60-61 -1.5246192272272374 2311 62-63 -2.0945659304549125 2311 64-65 -3.113197499890191 2311 66-67 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0.6563559540316319 2312 26-27 0.4526687859617837 2312 28-29 1.0312911399237379 2312 30-31 0.9877707352508978 2312 32-33 1.1224382791777145 2312 34-35 1.1686401743405952 2312 36-37 1.2404513933498649 2312 38-39 1.3056141305028035 2312 40-41 0.686837466185132 2312 42-43 1.2915643486763244 2312 44-45 1.5415582006867652 2312 46-47 1.7823230349151231 2312 48-49 2.2432533472153082 2312 50-51 2.3328060964042834 2312 52-53 2.9858632096770563 2312 54-55 2.652101910361708 2312 56-57 2.463256031351264 2312 58-59 3.4897758628569235 2312 60-61 2.3186556265739284 2312 62-63 1.538475005217606 2312 64-65 1.81955103812151 2312 66-67 2.207980245852003 2312 68-69 2.0646409819425884 2312 70-71 2.0317660639738406 2312 72-73 2.6239484581189068 2312 74-75 2.6853889390576917 2312 76-77 1.2016727408339065 2312 78-79 1.2122847336503604 2312 80-81 1.6743211821928625 2312 82-83 1.7978667603093221 2312 84-85 2.042597284472329 2312 86-87 1.8744447824213104 2312 88-89 1.9926429775970291 2312 90-91 1.90598375707809 2312 92-93 1.9586946706446504 2312 94-95 1.9503323975131721 2312 96-97 1.1448545463655186 2312 98-99 3.187200937749868 2312 100-101 3.2163989879692885 2313 1 0.754809212653246 2313 2 0.8672042938623292 2313 3 1.0149789964626166 2313 4 1.1607697536167052 2313 5 0.5781263409795869 2313 6 0.7551781229466386 2313 7 0.5716146715671186 2313 8 0.8826447159399535 2313 9 0.9606003388331601 2313 10-11 0.9058267126551485 2313 12-13 1.4226394960246935 2313 14-15 1.5959859269246977 2313 16-17 1.5520609785358985 2313 18-19 1.4799522616267637 2313 20-21 1.3611865058522028 2313 22-23 1.1401124660562019 2313 24-25 1.0024891441497843 2313 26-27 1.2767826420305184 2313 28-29 1.6233964030816352 2313 30-31 1.6972229371843 2313 32-33 1.9854887625289521 2313 34-35 1.7725606899152453 2313 36-37 2.1274009099986344 2313 38-39 2.3066882443588668 2313 40-41 2.3492971869155284 2313 42-43 1.536462307859999 2313 44-45 2.196499124424676 2313 46-47 2.3961791036584117 2313 48-49 2.214520801565463 2313 50-51 2.2468769879187818 2313 52-53 2.5261424792796348 2313 54-55 2.554089020995427 2313 56-57 3.006220585594015 2313 58-59 2.60202076081611 2313 60-61 3.1220927909133565 2313 62-63 3.183211847322866 2313 64-65 2.7824941100871357 2313 66-67 2.125810535862744 2313 68-69 1.9929438820499996 2313 70-71 2.0739250328245333 2313 72-73 2.955581111180127 2313 74-75 2.2995135362649926 2313 76-77 3.0617694110809524 2313 78-79 2.849234250299123 2313 80-81 3.5591224711294913 2313 82-83 3.0932480707282295 2313 84-85 2.784541430551812 2313 86-87 3.2082256631946713 2313 88-89 3.4891521075648058 2313 90-91 3.3702694713638053 2313 92-93 3.8802843591516307 2313 94-95 3.7626310011651576 2313 96-97 3.0500639985674525 2313 98-99 3.56958547051034 2313 100-101 2.2499650459714395 2314 1 -0.4255548248536485 2314 2 -0.1523765114327631 2314 3 -0.0865102478837727 2314 4 -0.3648783434599565 2314 5 0.23863378720131578 2314 6 -0.011506929452700376 2314 7 0.529143629096076 2314 8 -1.5263459073363848 2314 9 -0.3725491371734577 2314 10-11 -0.253991268591399 2314 12-13 0.2734392753959085 2314 14-15 0.23856728379177383 2314 16-17 0.4989721754470864 2314 18-19 0.1465269996300762 2314 20-21 0.3833872780529717 2314 22-23 0.02979807002752466 2314 24-25 -0.2753652408474565 2314 26-27 -0.30871101489170627 2314 28-29 0.36663964632487733 2314 30-31 -0.028232109699324326 2314 32-33 0.8379432578405925 2314 34-35 0.5431067461755816 2314 36-37 0.6398112795518571 2314 38-39 -0.008397249298056408 2314 40-41 0.4983595145492856 2314 42-43 0.16994272705470337 2314 44-45 -0.21235360585662022 2314 46-47 -0.22585619694831394 2314 48-49 -0.31471251338213335 2314 50-51 0.46047323722932276 2314 52-53 1.0989499806585599 2314 54-55 0.7093565334058027 2314 56-57 0.8487467852630672 2314 58-59 0.8816677547488112 2314 60-61 0.12057596796795167 2314 62-63 0.6696983338093503 2314 64-65 0.257673370980676 2314 66-67 0.908904854671345 2314 68-69 -0.06800482175584222 2314 70-71 -0.4266265391556061 2314 72-73 -0.15307856890812133 2314 74-75 0.1747203757575484 2314 76-77 -0.1973315265914124 2314 78-79 0.6656986739064052 2314 80-81 0.05305517934791126 2314 82-83 -0.13634376600646547 2314 84-85 -0.46380385350775555 2314 86-87 0.03020580660338723 2314 88-89 0.3358150970849394 2314 90-91 1.2640918651861988 2314 92-93 1.1855794501610077 2314 94-95 0.9876723690636702 2314 96-97 1.5235885435205674 2314 98-99 0.27752810702440556 2314 100-101 0.04712445027369583 2315 1 0.013312614013791801 2315 2 -0.0783739374301895 2315 3 -0.24352440489792926 2315 4 0.35294662436501056 2315 5 0.3808474294149633 2315 6 0.09531417736840808 2315 7 0.27399562394807475 2315 8 0.542508661518184 2315 9 0.2973350327107127 2315 10-11 0.08609882149869463 2315 12-13 0.4124354143920428 2315 14-15 0.4301696003940947 2315 16-17 0.2722990737739863 2315 18-19 0.3532515813546553 2315 20-21 0.0873769820426773 2315 22-23 0.408819949049402 2315 24-25 0.3621830217008082 2315 26-27 0.19514998896929825 2315 28-29 -0.30517502548979536 2315 30-31 0.3536855222183064 2315 32-33 -0.07148402658669539 2315 34-35 0.35504368311252676 2315 36-37 -1.5011040952828125E-4 2315 38-39 0.513320897420094 2315 40-41 0.7319502481400164 2315 42-43 0.017754824866798913 2315 44-45 -1.4684668619698797 2315 46-47 -1.4864739575660764 2315 48-49 -0.5703431440127673 2315 50-51 0.04959807635415814 2315 52-53 0.3373669690755463 2315 54-55 -0.9841762851270133 2315 56-57 -1.1451399586236732 2315 58-59 -1.5102241371430765 2315 60-61 -1.6678731954812065 2315 62-63 -2.108454819343798 2315 64-65 -1.9802609919536849 2315 66-67 -2.6488493280828393 2315 68-69 -2.789369043120061 2315 70-71 -2.5731838107128766 2315 72-73 -1.9474801133096626 2315 74-75 -2.253207552170384 2315 76-77 -1.9339788882387765 2315 78-79 -2.736650103442372 2315 80-81 -2.809074807782075 2315 82-83 -2.3744390041017027 2315 84-85 -2.4705606102645135 2315 86-87 -2.918475017077437 2315 88-89 -2.2523444910746484 2315 90-91 -1.814254338160005 2315 92-93 -2.126518361936803 2315 94-95 -2.466960835569534 2315 96-97 -2.296874776942751 2315 98-99 -2.2263328968365954 2315 100-101 -2.8716335934843435 2316 1 -0.31530596212953554 2316 2 -0.28818063238539793 2316 3 -0.5004786717531857 2316 4 0.038118713002440074 2316 5 0.35303036199393034 2316 6 -0.13005121631381655 2316 7 0.06807860367461416 2316 8 -0.02420515272038415 2316 9 -0.7910666646018782 2316 10-11 -0.42545233361681767 2316 12-13 -1.0154288006008798 2316 14-15 -1.3613791973428278 2316 16-17 -1.0377551459336942 2316 18-19 -0.6937191871507693 2316 20-21 -0.3184457430869756 2316 22-23 -1.448794383812455 2316 24-25 -0.54570240969948 2316 26-27 -0.44558551315234496 2316 28-29 -0.861752111769853 2316 30-31 -0.6998269718882426 2316 32-33 -1.4453053372891986 2316 34-35 -1.0331576370194853 2316 36-37 -0.8724980594901552 2316 38-39 -1.0140836287468105 2316 40-41 -0.8469040658628089 2316 42-43 -1.0450693146900107 2316 44-45 -1.22459604631689 2316 46-47 -1.9274216237612691 2316 48-49 -1.4189405037487361 2316 50-51 -1.2448385101616353 2316 52-53 -0.6884463265397258 2316 54-55 -0.9811706274183862 2316 56-57 -1.497921665365773 2316 58-59 -1.1389370084302044 2316 60-61 -1.42417918322284 2316 62-63 -2.094015875449408 2316 64-65 -2.267212901430348 2316 66-67 -1.8029629537311216 2316 68-69 -2.200731607947972 2316 70-71 -1.4334501944941138 2316 72-73 -1.9428832250494104 2316 74-75 -1.4322504564608103 2316 76-77 -1.1218030277955862 2316 78-79 -1.655615214239166 2316 80-81 -1.294282257098434 2316 82-83 -1.0893140630361664 2316 84-85 -1.4161051647199585 2316 86-87 -1.5892081618204834 2316 88-89 -1.8433393048417415 2316 90-91 -2.170748916189236 2316 92-93 -1.856834250668534 2316 94-95 -2.85203862906317 2316 96-97 -3.056422160252513 2316 98-99 -2.471736008576343 2316 100-101 -2.7803441073928816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 502.0 3 94.0 4 76.0 5 75.0 6 85.0 7 99.0 8 127.0 9 148.0 10 178.0 11 282.0 12 384.0 13 631.0 14 674.0 15 965.0 16 1364.0 17 1950.0 18 1707.0 19 957.0 20 827.0 21 886.0 22 816.0 23 971.0 24 953.0 25 1061.0 26 1117.0 27 1214.0 28 1391.0 29 1581.0 30 2075.0 31 2432.0 32 3118.0 33 4317.0 34 6284.0 35 9352.0 36 14966.0 37 24512.0 38 24682.0 39 4345.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93937035484892 20.064486884530844 15.859155170496821 21.136987590123415 2 14.320647759432953 20.65288952350009 42.44294911805193 22.583513599015024 3 17.847340384598944 26.632920916949544 31.952151719066634 23.56758697938488 4 11.337517529158259 17.698464274720386 40.10756917604405 30.8564490200773 5 11.510606824963874 38.196540490624436 36.09740665053483 14.195446033876857 6 28.125026709629996 38.31656680826332 18.9138368704006 14.644569611706082 7 25.077984121151005 34.795019271692404 22.813239780875303 17.313756826281292 8 25.102775142517714 34.35040127176226 21.34987991760895 19.196943668111075 9 25.4958935484698 16.658262898359983 22.28593892881866 35.55990462435156 10-11 23.18768725771363 27.242497937760984 29.06068752698007 20.509127277545318 12-13 24.81857578788112 25.512646271935445 29.317212435144587 20.35156550503885 14-15 21.737142368752803 26.226790025854147 29.11048909211342 22.925578513279632 16-17 19.42624667205121 29.826968030324398 29.598338482839964 21.148446814784425 18-19 19.21002700762367 27.590937061980785 33.511674814536256 19.687361115859286 20-21 19.979574744470654 26.439595938878064 33.76747683998222 19.81335247666906 22-23 19.62249799155599 26.46060885766542 34.08372220228023 19.83317094849837 24-25 19.48979809849375 26.53819036427732 34.1143040273475 19.857707509881422 26-27 19.306761468517124 26.654474601763862 34.068845286114716 19.96991864360429 28-29 19.084693615930263 27.22844201350312 34.00692248525767 19.67994188530895 30-31 19.426532487233725 26.835459265431705 33.76535692156486 19.972651325769718 32-33 18.924982053122758 27.12542303353502 33.72004580726763 20.22954910607459 34-35 19.549582273883036 27.08275464199483 33.60270079699152 19.764962287130615 36-37 19.41434358281915 26.904472969046438 33.4733450698207 20.207838378313706 38-39 19.095299145299148 27.415384615384614 33.437606837606836 20.051709401709402 40-41 19.108704848751596 27.55887342480739 33.532747341027864 19.799674385413148 42-43 18.983744701217013 27.798099275263226 32.704601394776425 20.513554628743332 44-45 19.15538216329164 27.781884686547905 32.52586284254112 20.53687030761934 46-47 19.487556406399563 27.9745829345002 31.995675509366883 20.542185149733353 48-49 19.514477642554358 28.240804047586487 31.487590592096264 20.757127717762888 50-51 19.547214316847423 28.60805579058021 31.504414190361423 20.34031570221094 52-53 19.77496495846296 28.552442651533283 30.936976513623467 20.735615876380294 54-55 19.416264262211016 28.258194094269474 31.34652365283535 20.97901799068416 56-57 19.91735819709258 28.164873387972072 31.0269974617771 20.89077095315825 58-59 19.884275482470386 27.772602177740552 31.415274952564914 20.927847387224148 60-61 19.84846995679727 27.57282715062838 31.83882946673903 20.739873425835317 62-63 19.618468060375037 27.54910172475684 32.12423719252662 20.708193022341497 64-65 19.324650017947796 27.329367724732066 32.27484060646463 21.071141650855512 66-67 19.19544994209872 27.369999871805277 31.680333648122588 21.754216537973413 68-69 19.14380824996902 27.095930567959897 32.214449624593506 21.545811557477577 70-71 19.215186843994665 27.10391808266949 31.87544873329003 21.805446340045815 72-73 19.239348745780095 26.79500876030939 32.03880176060852 21.926840733301994 74-75 18.999615401051237 27.13388316738601 31.848638947053544 22.01786248450921 76-77 19.013434519536457 26.504546542234987 31.919803097118244 22.562215841110316 78-79 19.03874750029911 25.86485377818039 32.24571418804587 22.850684533474627 80-81 18.884235716422378 26.200162386222807 32.16614674586556 22.749455151489254 82-83 18.890218366736462 26.679629075680527 32.21058929105594 22.21956326652707 84-85 18.732827102304533 26.433549699380816 31.390881859010243 23.44274133930441 86-87 18.652121336426994 26.45891286048191 31.459190606212108 23.429775196878992 88-89 18.570329858143907 25.397795248675443 31.88215689625705 24.149717996923602 90-91 18.85810487454707 25.21492787311137 32.21482532303275 23.712141929308814 92-93 18.662029005324197 25.611684171844157 32.04088434618376 23.685402476647894 94-95 18.126153688384495 25.55889792848841 32.11740274834211 24.197545634784987 96-97 18.081410998645573 26.41990711267395 31.876076172734535 23.622605715945944 98-99 18.729169871301853 26.868515954810373 30.09964278999812 24.302671383889656 100-101 18.735392017365072 26.745373515700777 29.574889011378737 24.944345455555414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 153.0 1 89.5 2 29.5 3 45.0 4 43.0 5 32.5 6 64.5 7 142.5 8 237.0 9 298.5 10 293.5 11 272.0 12 260.5 13 272.5 14 287.5 15 311.5 16 342.5 17 347.0 18 364.0 19 414.5 20 463.0 21 482.5 22 550.5 23 664.0 24 769.5 25 883.5 26 1077.5 27 1334.0 28 1531.0 29 1732.5 30 2064.5 31 2364.5 32 2588.0 33 2856.0 34 3195.0 35 3461.5 36 3719.0 37 4043.0 38 4172.5 39 4117.0 40 4129.0 41 4187.0 42 4184.0 43 4265.0 44 4332.0 45 4308.0 46 4295.0 47 4468.5 48 4781.0 49 4505.0 50 3839.5 51 3377.0 52 3028.0 53 2812.0 54 2600.0 55 2279.5 56 1935.5 57 1646.5 58 1416.5 59 1149.5 60 865.5 61 695.5 62 549.5 63 373.5 64 260.0 65 185.5 66 123.0 67 85.0 68 64.0 69 46.5 70 33.0 71 23.5 72 12.0 73 12.0 74 9.5 75 5.5 76 5.5 77 4.0 78 3.5 79 2.0 80 1.5 81 1.5 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23634812286689422 2 0.2073378839590444 3 0.21075085324232082 4 0.21501706484641636 5 0.21075085324232082 6 0.17150170648464164 7 0.1612627986348123 8 0.1680887372013652 9 0.1612627986348123 10-11 0.1838737201365188 12-13 0.17832764505119453 14-15 0.16851535836177475 16-17 0.16936860068259385 18-19 0.16723549488054607 20-21 0.16040955631399317 22-23 0.16552901023890787 24-25 0.1599829351535836 26-27 0.15699658703071673 28-29 0.1621160409556314 30-31 0.16424914675767918 32-33 0.16040955631399317 34-35 0.16851535836177475 36-37 0.15870307167235495 38-39 0.17064846416382254 40-41 0.16254266211604096 42-43 0.16382252559726962 44-45 0.16083617747440274 46-47 0.16382252559726962 48-49 0.16382252559726962 50-51 0.16296928327645052 52-53 0.16723549488054607 54-55 0.16638225255972697 56-57 0.1612627986348123 58-59 0.1689419795221843 60-61 0.16510238907849828 62-63 0.1689419795221843 64-65 0.16552901023890787 66-67 0.16254266211604096 68-69 0.16510238907849828 70-71 0.17406143344709896 72-73 0.16638225255972697 74-75 0.16638225255972697 76-77 0.16040955631399317 78-79 0.15870307167235495 80-81 0.16638225255972697 82-83 0.16638225255972697 84-85 0.16339590443686008 86-87 0.1591296928327645 88-89 0.15358361774744025 90-91 0.15699658703071673 92-93 0.15955631399317405 94-95 0.15699658703071673 96-97 0.15059726962457337 98-99 0.1552901023890785 100-101 0.15656996587030717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 117200.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.72269624573379 #Duplication Level Percentage of deduplicated Percentage of total 1 90.70489949610828 70.49829351535836 2 5.512070347235182 8.56825938566553 3 1.5325334006652689 3.573378839590444 4 0.7146699454391763 2.2218430034129693 5 0.4017960061916106 1.5614334470989761 6 0.2162672492342822 1.0085324232081911 7 0.17674633059248443 0.96160409556314 8 0.14381223172431964 0.89419795221843 9 0.10429131308252189 0.7295221843003413 >10 0.4621751874499127 5.924061433447099 >50 0.017564852729687892 0.8165529010238908 >100 0.00988022966044944 1.1638225255972696 >500 0.0021956065912109865 1.18259385665529 >1k 0.0010978032956054933 0.8959044368600683 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1050 0.8959044368600683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 864 0.7372013651877133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 522 0.4453924914675768 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 212 0.18088737201365188 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.17832764505119453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 179 0.15273037542662116 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 156 0.13310580204778155 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 142 0.12116040955631399 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 121 0.10324232081911262 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 119 0.10153583617747441 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0017064846416382253 0.0 26-27 0.0 0.0 0.0 0.002559726962457338 0.0 28-29 0.0 0.0 0.0 0.006825938566552902 0.0 30-31 0.0 0.0 0.0 0.014505119453924914 0.0 32-33 0.0 0.0 0.0 0.022610921501706488 0.0 34-35 0.0 0.0 0.0 0.032423208191126277 0.0 36-37 0.0 0.0 0.0 0.04436860068259386 0.0 38-39 0.0 0.0 0.0 0.05332764505119454 0.0 40-41 0.0 0.0 0.0 0.06655290102389078 0.0 42-43 0.0 0.0 0.0 0.08532423208191126 0.0 44-45 0.0 0.0 0.0 0.1454778156996587 0.0 46-47 0.0 0.0 0.0 0.1924061433447099 0.0 48-49 0.0 0.0 0.0 0.318259385665529 0.0 50-51 0.0 0.0 0.0 0.4445392491467577 0.0 52-53 0.0 0.0 0.0 0.5878839590443686 0.0 54-55 8.532423208191126E-4 0.0 0.0 0.8805460750853242 0.0 56-57 8.532423208191126E-4 0.0 0.0 1.2141638225255973 0.0 58-59 8.532423208191126E-4 0.0 0.0 1.627986348122867 0.0 60-61 8.532423208191126E-4 0.0 0.0 2.131399317406143 0.0 62-63 8.532423208191126E-4 0.0 0.0 2.8630546075085324 0.0 64-65 8.532423208191126E-4 0.0 0.0 3.3609215017064846 0.0 66-67 8.532423208191126E-4 0.0 0.0 4.041382252559727 0.0 68-69 8.532423208191126E-4 0.0 0.0 4.824232081911262 0.0 70-71 8.532423208191126E-4 0.0 0.0 5.472269624573379 0.0 72-73 8.532423208191126E-4 0.0 0.0 6.622013651877133 0.0 74-75 8.532423208191126E-4 0.0 0.0 7.886092150170649 0.0 76-77 8.532423208191126E-4 0.0 0.0 8.953924914675769 0.0 78-79 8.532423208191126E-4 0.0 0.0 9.906143344709896 0.0 80-81 8.532423208191126E-4 0.0 0.0 11.126279863481228 0.0 82-83 8.532423208191126E-4 0.0 0.0 12.231228668941979 0.0 84-85 8.532423208191126E-4 0.0 0.0 13.456058020477816 0.0 86-87 8.532423208191126E-4 0.0 0.0 15.426621160409557 0.0 88-89 8.532423208191126E-4 0.0 0.0 18.0759385665529 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCAT 45 2.0300831E-7 63.340454 5 TTAGGCA 40 5.365264E-4 47.50534 4 TTAGGGC 50 0.0016090004 38.004272 3 GTATAGC 65 1.3167129E-4 36.558193 1 ATGGGAT 175 0.0 35.289684 5 GCATTCC 55 0.0025687884 34.54934 8 CAGTGCA 70 2.0407706E-4 33.93239 9 GGTATCA 830 0.0 33.210693 1 ATGGGGA 85 6.3683867E-4 27.944319 5 ACAGTGC 70 0.008356751 27.14591 8 CTACACT 70 0.008356751 27.14591 4 GTACATG 530 0.0 26.901312 1 TACATGG 555 0.0 26.534515 2 GTATCAA 1150 0.0 24.795994 1 GAGATGG 135 1.3282108E-5 24.6324 9 GAGTACT 765 0.0 24.218409 12-13 CATGGGA 370 0.0 23.110708 4 ACATGGG 540 0.0 22.872944 3 ATTAGGG 105 0.0021831086 22.621592 2 CAGAGTG 105 0.0021831086 22.621592 4 >>END_MODULE