FastQCFastQC Report
Thu 26 May 2016
SRR937322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117200
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.787542662116041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.6962457337883958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.45819112627986347No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4340.3703071672354949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.20477815699658702No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.19197952218430034No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC1660.1416382252559727No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1520.12969283276450513No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1440.12286689419795221No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1210.10324232081911262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG752.3483153E-950.6816865
AGGTAAG601.4777233E-647.5140882
GTAAGGT601.4777233E-647.5140884
ATACACC405.3717254E-447.4938138
AAGGTGT751.3501995E-744.3275576
GTCCTGG459.555566E-442.2527771
CTATCCC459.5756794E-442.2347454
GGTGTGC951.9052095E-839.994798
GTATTCA608.210122E-539.6119731
GACTGAT500.00161093137.9950527
AGGTGTG905.645725E-736.9396367
GACTATG550.002571865534.5409557
TATATGG702.0385816E-433.9386332
TAATCGG352.0499337E-433.92415614-15
GGTAAGG851.6164917E-533.5393523
GTCCAAG753.048754E-431.6895791
TAAAGAG600.003930900731.6760585
ACTGTGC600.003939127531.6625448
AATCGGA300.0039471131.66254416-17
AAGGTAA1004.8915517E-528.5206221
Produced by FastQC (version 0.11.5)