##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937322_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117200 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4644795221843 33.0 31.0 34.0 31.0 34.0 2 32.66536689419795 34.0 31.0 34.0 31.0 34.0 3 32.73034129692833 34.0 31.0 34.0 31.0 34.0 4 36.28851535836178 37.0 35.0 37.0 35.0 37.0 5 36.20517918088737 37.0 35.0 37.0 35.0 37.0 6 36.30975255972696 37.0 37.0 37.0 35.0 37.0 7 36.28028156996587 37.0 37.0 37.0 35.0 37.0 8 36.2660409556314 37.0 37.0 37.0 35.0 37.0 9 38.01709044368601 39.0 38.0 39.0 35.0 39.0 10-11 37.942034982935155 39.0 38.0 39.0 35.0 39.0 12-13 37.8757295221843 39.0 38.0 39.0 35.0 39.0 14-15 39.12290529010239 40.0 38.0 41.0 36.0 41.0 16-17 39.12686006825939 40.0 38.0 41.0 36.0 41.0 18-19 39.177508532423204 40.0 38.5 41.0 36.0 41.0 20-21 39.12694965870307 40.0 39.0 41.0 36.0 41.0 22-23 39.00758105802048 40.0 38.0 41.0 35.5 41.0 24-25 38.86091296928328 40.0 38.0 41.0 35.0 41.0 26-27 38.64761945392492 40.0 38.0 41.0 35.0 41.0 28-29 38.62234641638226 40.0 38.0 41.0 35.0 41.0 30-31 38.682555460750855 40.0 38.0 41.0 35.0 41.0 32-33 38.575375426621164 40.0 38.0 41.0 35.0 41.0 34-35 38.43499146757679 40.0 38.0 41.0 35.0 41.0 36-37 38.27296501706485 40.0 38.0 41.0 34.5 41.0 38-39 38.087768771331056 40.0 38.0 41.0 34.0 41.0 40-41 37.93112201365187 40.0 38.0 41.0 34.0 41.0 42-43 37.69100682593857 40.0 37.0 41.0 33.0 41.0 44-45 37.28009812286689 40.0 37.0 41.0 33.0 41.0 46-47 36.75962030716724 40.0 37.0 41.0 31.5 41.0 48-49 36.2684385665529 40.0 36.5 41.0 30.5 41.0 50-51 35.98226535836177 40.0 36.0 41.0 29.5 41.0 52-53 35.744726962457335 40.0 36.0 41.0 28.0 41.0 54-55 35.52711177474403 39.0 35.0 41.0 28.0 41.0 56-57 35.25547781569966 39.0 35.0 41.0 26.5 41.0 58-59 34.996232935153586 39.0 35.0 40.5 26.0 41.0 60-61 34.65782849829351 38.5 35.0 40.0 25.0 41.0 62-63 34.332781569965874 38.0 34.5 40.0 23.5 41.0 64-65 33.99117747440273 38.0 34.0 40.0 22.0 41.0 66-67 33.59275170648465 37.0 34.0 40.0 21.0 41.0 68-69 33.14269197952218 36.5 34.0 39.0 18.5 41.0 70-71 32.707389078498295 36.0 33.0 39.0 13.0 40.0 72-73 32.25029863481229 35.5 33.0 39.0 7.5 40.0 74-75 31.815524744027304 35.0 32.0 38.0 4.0 40.0 76-77 30.524914675767917 34.0 30.5 36.0 2.0 39.0 78-79 31.055669795221846 35.0 32.0 37.0 2.0 39.0 80-81 30.840529010238907 35.0 32.0 36.0 2.0 38.5 82-83 30.498110068259386 35.0 31.5 36.0 2.0 37.5 84-85 30.15409129692833 35.0 31.0 35.5 2.0 37.0 86-87 29.871220136518772 34.0 31.0 35.0 2.0 36.5 88-89 29.55443686006826 34.0 31.0 35.0 2.0 36.0 90-91 29.152043515358365 34.0 30.5 35.0 2.0 36.0 92-93 28.793579351535836 34.0 30.0 35.0 2.0 35.0 94-95 28.436232935153583 34.0 29.0 35.0 2.0 35.0 96-97 28.08851962457338 34.0 29.0 35.0 2.0 35.0 98-99 27.548421501706486 34.0 29.0 35.0 2.0 35.0 100-101 26.100686860068258 32.5 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.09553704064271784 1101 2 -0.06311987229418747 1101 3 -0.032004853316358606 1101 4 -0.0012992007481713586 1101 5 -0.15232865372693993 1101 6 0.014077397237180378 1101 7 -0.0847749621313838 1101 8 0.053265940780306664 1101 9 0.013929134990519287 1101 10-11 0.05777356169802772 1101 12-13 0.23392120307008923 1101 14-15 0.2763358627149941 1101 16-17 0.04057055232372164 1101 18-19 -0.23898299581536975 1101 20-21 -0.22983414073856778 1101 22-23 -0.2809739548381245 1101 24-25 -0.5433933699152149 1101 26-27 0.09446603441873691 1101 28-29 -0.058951680244483384 1101 30-31 0.06294436610459542 1101 32-33 0.22552437766005085 1101 34-35 0.2846842385667898 1101 36-37 0.3340607231832209 1101 38-39 -0.009576158181232586 1101 40-41 0.41522710728144574 1101 42-43 0.5496427522625567 1101 44-45 0.8213891167125027 1101 46-47 0.8882493589836287 1101 48-49 1.0041457910027063 1101 50-51 1.0933504490991197 1101 52-53 1.2865712834273921 1101 54-55 0.8315742193890046 1101 56-57 1.1429758443572737 1101 58-59 1.5184420282556772 1101 60-61 1.5657734008421116 1101 62-63 1.774917073087387 1101 64-65 1.6761451699975254 1101 66-67 1.4586508583386006 1101 68-69 1.5478924380255066 1101 70-71 1.0782255534106682 1101 72-73 1.5593044126947397 1101 74-75 1.6259038472288516 1101 76-77 1.7177096400835374 1101 78-79 1.810235255857659 1101 80-81 1.8277789253761405 1101 82-83 1.4517929127193803 1101 84-85 1.5598386432733307 1101 86-87 1.357593047380334 1101 88-89 1.2446585306385352 1101 90-91 1.4871350184830554 1101 92-93 1.3169977887264537 1101 94-95 0.8029258749724271 1101 96-97 1.3091485559066207 1101 98-99 1.1984008930387517 1101 100-101 1.3917008053330733 1102 1 -0.1662951962833219 1102 2 -0.4049277818987065 1102 3 -0.17033180793636404 1102 4 -0.281142466649392 1102 5 -0.358133282850325 1102 6 -0.24075219999263453 1102 7 -0.023311503046279825 1102 8 -0.266125347719786 1102 9 -0.05063255627975849 1102 10-11 -0.11781191306049266 1102 12-13 -0.07192671017940455 1102 14-15 -0.06683891403003628 1102 16-17 -0.2470638562784302 1102 18-19 -0.29074169820282947 1102 20-21 -0.05967057230407846 1102 22-23 -0.310714705703802 1102 24-25 -0.08393237119095431 1102 26-27 -0.3226562546281073 1102 28-29 -0.5089129523421718 1102 30-31 -0.2710496196799923 1102 32-33 0.0678676435612715 1102 34-35 0.04217922672061292 1102 36-37 0.24726465976058876 1102 38-39 0.014970497550500284 1102 40-41 -0.27108827453010775 1102 42-43 0.05978035022136652 1102 44-45 0.3260820272282672 1102 46-47 0.09873550806327813 1102 48-49 0.13480689907633092 1102 50-51 0.7093063448527204 1102 52-53 1.2075208004676625 1102 54-55 1.0271387355180366 1102 56-57 0.9079658206649128 1102 58-59 0.6192826515470955 1102 60-61 0.7164021597267478 1102 62-63 1.0629843229506974 1102 64-65 0.8658093763033392 1102 66-67 0.49727624561762696 1102 68-69 0.7690173696821532 1102 70-71 0.7025649009594161 1102 72-73 0.9673939880546776 1102 74-75 0.6191948076808309 1102 76-77 0.22094455898275456 1102 78-79 1.1752001729343924 1102 80-81 1.2175980432912148 1102 82-83 0.9931748153984401 1102 84-85 1.2035191608603562 1102 86-87 1.0634204576227262 1102 88-89 1.1858149002394107 1102 90-91 0.8788905315138571 1102 92-93 0.76486319787535 1102 94-95 1.063257112442816 1102 96-97 1.0106475535373853 1102 98-99 0.7481092947154409 1102 100-101 1.3229701692842326 1103 1 -0.12273392607097122 1103 2 -0.03898194125970633 1103 3 0.05854019673925848 1103 4 -0.15825136982492438 1103 5 -0.0362552388214894 1103 6 0.134155261530843 1103 7 -0.0931731823761055 1103 8 -0.10368622829644636 1103 9 -0.00792848458679174 1103 10-11 -0.00561631594379719 1103 12-13 -0.10327290149052715 1103 14-15 -0.09908126742961088 1103 16-17 -0.26660408616348974 1103 18-19 -0.2927093584405114 1103 20-21 0.1064700861802308 1103 22-23 -0.5287353563954085 1103 24-25 -0.4431569961666071 1103 26-27 -0.42205231930762466 1103 28-29 -0.0879700339708478 1103 30-31 0.0056306842358466724 1103 32-33 0.1863558570816295 1103 34-35 0.1897732263309777 1103 36-37 0.5674867521042444 1103 38-39 0.8564277350334493 1103 40-41 0.5186058614527482 1103 42-43 0.620193364053435 1103 44-45 0.9689419531974934 1103 46-47 1.2097037527544856 1103 48-49 1.0460673705355177 1103 50-51 1.3860645759066799 1103 52-53 1.81977484293796 1103 54-55 1.5133456320697576 1103 56-57 1.3962572681615057 1103 58-59 1.4872545977773726 1103 60-61 1.6401894186396717 1103 62-63 2.2733875958905045 1103 64-65 1.933917695025336 1103 66-67 2.3190373989392654 1103 68-69 2.8465159085483194 1103 70-71 2.5976360094729642 1103 72-73 2.1535619210373405 1103 74-75 2.4280590196426424 1103 76-77 1.3510244342993296 1103 78-79 1.9531857564138413 1103 80-81 1.8763745872226387 1103 82-83 1.8209948037093682 1103 84-85 1.9224359736404075 1103 86-87 2.19260417085086 1103 88-89 2.634675215844318 1103 90-91 2.5710198905631465 1103 92-93 2.8289972325529256 1103 94-95 2.128384718131496 1103 96-97 2.010511181045665 1103 98-99 1.56141535355043 1103 100-101 0.804131283642306 1104 1 -0.15752548551513001 1104 2 0.16076072472073832 1104 3 0.011808338787638206 1104 4 -0.06653752525467382 1104 5 -0.07830361761716631 1104 6 0.07630657393433893 1104 7 0.023193261267749676 1104 8 0.017878362542461446 1104 9 0.12526760393611625 1104 10-11 0.32459469795223583 1104 12-13 0.09739616696032272 1104 14-15 0.1751081304087876 1104 16-17 0.16214012382106802 1104 18-19 0.25572090404018155 1104 20-21 0.07183292714780976 1104 22-23 0.3252790543096893 1104 24-25 0.02025010625233392 1104 26-27 -0.22534619475796092 1104 28-29 -0.5457415213614851 1104 30-31 -0.4020893363508762 1104 32-33 -0.3818119246991216 1104 34-35 -0.23294936759594265 1104 36-37 -0.41666147229102535 1104 38-39 -0.866912460540405 1104 40-41 -0.840761096859147 1104 42-43 -0.8802441037798161 1104 44-45 -0.802828504857068 1104 46-47 -0.46752218651468525 1104 48-49 -0.5267581569992146 1104 50-51 -0.9379446881180229 1104 52-53 -1.3258504787295706 1104 54-55 -0.6930105182133133 1104 56-57 -0.7878137560278944 1104 58-59 -0.6768215730924112 1104 60-61 -0.6341543796104574 1104 62-63 -0.8381667015876246 1104 64-65 -0.5342243533534585 1104 66-67 -0.4690995027591356 1104 68-69 -0.1423157950028937 1104 70-71 -0.30658426844777864 1104 72-73 -0.3883063239446436 1104 74-75 -0.43473048112935686 1104 76-77 -0.24184741335892923 1104 78-79 0.09258359480807243 1104 80-81 0.06577242561687413 1104 82-83 0.2206602694839468 1104 84-85 -0.25136742316865224 1104 86-87 0.14713324959506835 1104 88-89 -0.05330728544235086 1104 90-91 0.340860343471018 1104 92-93 0.6319051069739245 1104 94-95 0.1555077237928444 1104 96-97 0.23398004362929825 1104 98-99 0.5947658424850673 1104 100-101 0.03403349671487277 1105 1 -0.552497991406721 1105 2 -0.46838303018892447 1105 3 -0.4975396580701741 1105 4 -0.5281243280826331 1105 5 -0.32881422248755143 1105 6 -0.2204726876524603 1105 7 -0.690632347530368 1105 8 -0.3893486602383689 1105 9 -0.44676696008588124 1105 10-11 -0.4549811072323706 1105 12-13 -0.23104059659034704 1105 14-15 -0.42485259229349026 1105 16-17 -0.217239294874922 1105 18-19 -0.4362665272245394 1105 20-21 -0.7256533257979854 1105 22-23 -0.4321836322574768 1105 24-25 -0.5839918419016357 1105 26-27 -0.6436494154963768 1105 28-29 -0.27272502489643813 1105 30-31 -0.6764019836407371 1105 32-33 -0.3746241792160063 1105 34-35 -0.7884481892770125 1105 36-37 -1.720353538276882 1105 38-39 -1.2176194518994023 1105 40-41 -1.2031727229392644 1105 42-43 -1.1739990134420282 1105 44-45 -0.635232275477648 1105 46-47 -0.9040893506937877 1105 48-49 -1.0007565859073182 1105 50-51 -0.7549517580316092 1105 52-53 -0.5804066452653132 1105 54-55 -1.0307560013240646 1105 56-57 -0.821710063961909 1105 58-59 -1.0599323350719914 1105 60-61 -0.579048330906609 1105 62-63 -0.7456686654525129 1105 64-65 -0.996753811326748 1105 66-67 -1.2508355593184461 1105 68-69 -1.2258978845551347 1105 70-71 -1.3361207963346615 1105 72-73 -1.0321005109045807 1105 74-75 -0.245806679086936 1105 76-77 -0.579828560256022 1105 78-79 -1.0128759495752746 1105 80-81 -0.7423186977138414 1105 82-83 -0.4572266118986157 1105 84-85 -0.5708489628387241 1105 86-87 -0.24151561136330102 1105 88-89 -0.5691360364242861 1105 90-91 -0.8084719424677083 1105 92-93 -0.5882441467574182 1105 94-95 -0.5001089297813905 1105 96-97 -0.5744252980831916 1105 98-99 0.1939017419351856 1105 100-101 0.47527465932288493 1106 1 -0.03144535982777796 1106 2 0.16056433823212757 1106 3 -0.09227650017543709 1106 4 0.05345461928578743 1106 5 -0.1998668540664994 1106 6 -0.04152531923141112 1106 7 0.1041044945748979 1106 8 -0.03671708129100182 1106 9 0.08481198728254213 1106 10-11 0.16738731382026373 1106 12-13 0.2255383507780735 1106 14-15 0.7988316182328248 1106 16-17 0.14986596828297394 1106 18-19 0.5018913675123002 1106 20-21 0.13487298999148578 1106 22-23 -0.27428889541536705 1106 24-25 2.1868441415762163E-4 1106 26-27 -0.03180731023321215 1106 28-29 -0.03719870910696699 1106 30-31 0.11175591109610394 1106 32-33 -0.4180452318475929 1106 34-35 -0.05423766296122068 1106 36-37 0.012541198565216405 1106 38-39 -0.3044615571625613 1106 40-41 -0.022909565044535896 1106 42-43 -0.017420066073611906 1106 44-45 -0.006284907056596012 1106 46-47 0.5630159124641025 1106 48-49 0.5689802561979533 1106 50-51 0.08846929459996034 1106 52-53 0.6116986178925856 1106 54-55 0.3297703144654065 1106 56-57 0.7217109886696704 1106 58-59 0.45848871755958953 1106 60-61 0.7130569322512841 1106 62-63 1.1622260713895969 1106 64-65 0.14271987288377375 1106 66-67 0.674164440681551 1106 68-69 -0.05747683192355879 1106 70-71 -0.32033132265044983 1106 72-73 -1.142626826694059 1106 74-75 -1.1010698369816758 1106 76-77 -0.4679864549928645 1106 78-79 -0.5589285811542233 1106 80-81 -0.276529224029634 1106 82-83 -0.3664371382144047 1106 84-85 -0.8484805417860919 1106 86-87 -1.340199821889616 1106 88-89 -1.1375570890558642 1106 90-91 -0.7400508898361302 1106 92-93 -0.9829809888626819 1106 94-95 -0.6882668245182337 1106 96-97 -0.41389898229371624 1106 98-99 -0.3310982580648165 1106 100-101 0.15422202774394123 1107 1 -0.06680720193303813 1107 2 -6.198722941874735E-4 1107 3 0.04259192087719299 1107 4 0.005757250864732555 1107 5 -0.004143169855971962 1107 6 -0.08017663502088368 1107 7 -0.23800076858299946 1107 8 -0.15842760760678942 1107 9 0.13086461886148726 1107 10-11 0.20192678750447612 1107 12-13 0.04667321919912837 1107 14-15 -0.0926157501882301 1107 16-17 0.0836653103882341 1107 18-19 -0.047450737750857286 1107 20-21 0.1015670689388557 1107 22-23 -0.6587461322574768 1107 24-25 0.028179210729945225 1107 26-27 -0.05195533654900686 1107 28-29 -0.5725605512122272 1107 30-31 -0.4778822467986359 1107 32-33 -0.21121957395285307 1107 34-35 -0.6266060840138508 1107 36-37 -0.750616696171619 1107 38-39 -0.7119451097941365 1107 40-41 -0.36501482820242614 1107 42-43 -0.010841118705187114 1107 44-45 0.5224979876802465 1107 46-47 1.1974731493062123 1107 48-49 0.6150328877768985 1107 50-51 0.71305811038944 1107 52-53 1.1285571705241608 1107 54-55 0.9568262355180366 1107 56-57 0.7344576991959855 1107 58-59 0.5378472701911647 1107 60-61 1.1711161427775991 1107 62-63 1.750219492442227 1107 64-65 1.1118810570943012 1107 66-67 1.187733519628921 1107 68-69 -0.246210384555134 1107 70-71 -0.13447605949255603 1107 72-73 -0.11836695827300403 1107 74-75 0.3134038472288516 1107 76-77 0.1862076239545054 1107 78-79 0.36870299779314664 1107 80-81 0.6465793286019483 1107 82-83 0.3905529933645404 1107 84-85 0.2818648529507506 1107 86-87 0.9233692570577539 1107 88-89 1.0581665951546633 1107 90-91 0.10986687332176714 1107 92-93 -0.1741816467574182 1107 94-95 0.49471014916597866 1107 96-97 0.9253279913904926 1107 98-99 0.4143787156193959 1107 100-101 0.2755213698492014 1108 1 0.1491602638203844 1108 2 0.025921223596220955 1108 3 -0.04216835309541267 1108 4 0.08067163442637337 1108 5 -0.26998221095185926 1108 6 0.20000315949966563 1108 7 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-1.2736642067446127 2316 58-59 -1.749822903076165 2316 60-61 -1.528605391875864 2316 62-63 -1.5661480818152 2316 64-65 -1.515826244885897 2316 66-67 -1.3536495249255367 2316 68-69 -1.4357988746541466 2316 70-71 -1.235651802066812 2316 72-73 -0.8854616364908274 2316 74-75 -0.9229822913850079 2316 76-77 -1.3695627968375739 2316 78-79 -0.9258514576523993 2316 80-81 -1.163058666447558 2316 82-83 -1.1124637145562524 2316 84-85 -0.8464612609106332 2316 86-87 -0.6625682429422461 2316 88-89 -0.6104596424690989 2316 90-91 -0.30883671578714456 2316 92-93 -0.7268580081435587 2316 94-95 -0.7672793310320394 2316 96-97 -1.4916738650451506 2316 98-99 -1.7863483883410005 2316 100-101 -1.1688629123290184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 7.0 7 10.0 8 33.0 9 51.0 10 78.0 11 130.0 12 256.0 13 424.0 14 518.0 15 757.0 16 1149.0 17 1979.0 18 1762.0 19 918.0 20 715.0 21 663.0 22 650.0 23 685.0 24 713.0 25 745.0 26 919.0 27 938.0 28 1150.0 29 1406.0 30 1697.0 31 2145.0 32 2728.0 33 3800.0 34 5773.0 35 9018.0 36 15147.0 37 27185.0 38 29235.0 39 3813.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03248604448694 21.215794055890335 15.940866180713225 19.810853718909506 2 15.62542662116041 22.102389078498295 40.64675767918089 21.62542662116041 3 19.165529010238906 27.274744027303754 31.755119453924912 21.804607508532424 4 12.255972696245735 18.97013651877133 39.28668941979522 29.487201365187715 5 12.406031076941455 39.516012048501196 34.287884088640105 13.790072785917248 6 29.06313993174062 39.079351535836174 17.487201365187712 14.370307167235493 7 26.139931740614337 35.92576791808874 21.151023890784984 16.783276450511945 8 26.15699658703072 35.52303754266212 19.55631399317406 18.763651877133107 9 26.87713310580205 17.293515358361773 21.006825938566553 34.822525597269625 10-11 24.1339590443686 28.658276450511945 27.207764505119453 20.0 12-13 25.82479702718961 26.36150705445175 27.992542375773816 19.821153542584828 14-15 22.33831058020478 27.429180887372013 27.67320819112628 22.55930034129693 16-17 20.23848122866894 30.918088737201366 27.938139931740615 20.905290102389078 18-19 20.054180887372013 28.428327645051194 32.33703071672355 19.180460750853243 20-21 21.17960750853242 26.94411262798635 32.596416382252556 19.279863481228666 22-23 20.81580586533785 27.480652235125476 32.230347193187534 19.47319470634914 24-25 20.756061245995078 27.577336080785326 32.498005537566286 19.168597135653307 26-27 20.367929076380268 27.845541462415685 32.6242677895671 19.16226167163695 28-29 19.783098399262773 28.410525956517287 32.67645653435271 19.12991910986723 30-31 20.35810885758411 27.896569083353956 32.088584373586805 19.656737685475132 32-33 20.034129692832764 28.377986348122867 31.811433447098974 19.77645051194539 34-35 20.360921501706486 28.14675767918089 32.257252559726965 19.235068259385667 36-37 21.005635689572056 27.767183307096023 32.01464170068985 19.212539302642075 38-39 20.151109859683704 28.500974833510384 32.20320905805912 19.144706248746797 40-41 20.39334470989761 28.62542662116041 31.862627986348123 19.118600682593858 42-43 20.23876062924263 28.979806038988464 31.21338697909778 19.56804635267112 44-45 20.658276450511945 28.728242320819113 30.832764505119453 19.78071672354949 46-47 20.717150170648463 29.444965870307165 30.212030716723547 19.62585324232082 48-49 20.898890784982935 29.687713310580204 29.668941979522184 19.744453924914676 50-51 20.58319112627986 29.931313993174065 29.672354948805463 19.813139931740615 52-53 20.653671730681445 30.292364728518468 29.073502873305774 19.980460667494317 54-55 20.453498293515356 30.09641638225256 28.974829351535835 20.475255972696246 56-57 21.024744027303754 29.62542662116041 29.33319112627986 20.016638225255974 58-59 20.965870307167236 29.29650170648464 29.452645051194537 20.284982935153582 60-61 20.37286689419795 29.54650170648464 29.901023890784984 20.179607508532424 62-63 20.38438566552901 29.196245733788395 30.452645051194537 19.966723549488055 64-65 20.168515358361773 29.386518771331062 29.916808873720136 20.52815699658703 66-67 20.187713310580204 29.04607508532423 29.737627986348127 21.02858361774744 68-69 20.13651877133106 28.82721843003413 30.0 21.036262798634812 70-71 20.387798634812288 29.229948805460747 29.763225255972696 20.619027303754265 72-73 20.113907849829353 28.464590443686006 29.97482935153584 21.446672354948806 74-75 20.115187713310583 28.771757679180887 29.29863481228669 21.81441979522184 76-77 19.66552901023891 28.39675767918089 29.85238907849829 22.085324232081913 78-79 19.705631399317404 27.8080204778157 29.794795221843 22.69155290102389 80-81 19.74744027303754 28.23976109215017 29.865187713310583 22.147610921501705 82-83 19.89086984159489 28.65839871330509 29.420347356430703 22.03038408866932 84-85 19.505546075085327 28.878839590443683 28.88310580204778 22.73250853242321 86-87 19.0712457337884 28.759812286689417 28.42448805460751 23.744453924914676 88-89 19.091723549488055 28.06868600682594 29.01066552901024 23.828924914675767 90-91 19.443294834434827 27.772539348661784 29.192461781985585 23.591704034917804 92-93 19.214147059450926 27.81373322809787 28.614518227777896 24.357601484673307 94-95 19.267064846416382 28.701791808873722 28.257252559726965 23.773890784982935 96-97 19.546075085324233 29.464163822525595 27.716296928327644 23.273464163822524 98-99 19.275761738581387 30.197356632735772 26.06250906577701 24.464372562905826 100-101 19.18651039155534 30.211671139593726 24.91797263336647 25.683845835484465 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 84.0 1 55.5 2 32.0 3 38.0 4 35.5 5 40.5 6 56.5 7 110.5 8 170.5 9 222.5 10 250.0 11 238.5 12 247.5 13 279.0 14 307.5 15 327.0 16 339.0 17 352.5 18 364.0 19 380.5 20 419.0 21 472.0 22 514.0 23 564.0 24 683.0 25 828.5 26 1021.5 27 1252.5 28 1397.0 29 1623.0 30 1995.5 31 2306.5 32 2574.0 33 2810.5 34 3037.0 35 3330.0 36 3742.5 37 3947.5 38 3952.5 39 4027.5 40 4092.5 41 4188.0 42 4135.0 43 4197.5 44 4355.5 45 4256.0 46 4180.5 47 4487.5 48 4970.0 49 4592.0 50 3855.0 51 3460.0 52 3107.0 53 2896.0 54 2783.0 55 2531.0 56 2131.5 57 1867.5 58 1657.0 59 1319.5 60 1012.0 61 815.0 62 606.0 63 410.5 64 265.5 65 180.5 66 128.5 67 101.5 68 74.0 69 35.5 70 22.0 71 19.0 72 14.5 73 13.0 74 10.5 75 12.0 76 10.0 77 8.5 78 7.0 79 1.0 80 1.5 81 1.0 82 0.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03583617747440273 2 0.0 3 0.0 4 0.0 5 0.005972696245733789 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038395904436860067 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002559726962457338 24-25 4.266211604095563E-4 26-27 0.0029863481228668944 28-29 0.0034129692832764505 30-31 8.532423208191126E-4 32-33 0.0 34-35 0.0 36-37 4.266211604095563E-4 38-39 4.266211604095563E-4 40-41 0.0 42-43 0.009812286689419795 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.266211604095563E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.266211604095563E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008959044368600682 92-93 0.0021331058020477816 94-95 0.0 96-97 0.0 98-99 8.532423208191126E-4 100-101 0.01151877133105802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 117200.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.75426621160409 #Duplication Level Percentage of deduplicated Percentage of total 1 89.72857599415606 67.0759385665529 2 6.063096379491394 9.064846416382252 3 1.6618728028124004 3.726962457337884 4 0.794411724421312 2.3754266211604094 5 0.4576998584668767 1.7107508532423206 6 0.27393507738665934 1.228668941979522 7 0.18376478108021732 0.96160409556314 8 0.13696753869332967 0.8191126279863481 9 0.099301465552664 0.6680887372013652 >10 0.5535771355522074 7.248293515358362 >50 0.03310048851755467 1.726962457337884 >100 0.010272565401999726 1.4513651877133107 >500 0.003424188467333242 1.9419795221843001 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.787542662116041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.6962457337883958 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 537 0.45819112627986347 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 434 0.3703071672354949 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 240 0.20477815699658702 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 225 0.19197952218430034 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 166 0.1416382252559727 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 152 0.12969283276450513 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 144 0.12286689419795221 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 121 0.10324232081911262 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0017064846416382253 0.0 26-27 0.0 0.0 0.0 0.002559726962457338 0.0 28-29 0.0 0.0 0.0 0.006825938566552902 0.0 30-31 0.0 0.0 0.0 0.014505119453924914 0.0 32-33 0.0 0.0 0.0 0.0234641638225256 0.0 34-35 0.0 0.0 0.0 0.03370307167235495 0.0 36-37 0.0 0.0 0.0 0.04522184300341297 0.0 38-39 0.0 0.0 0.0 0.05332764505119454 0.0 40-41 0.0 0.0 0.0 0.06655290102389078 0.0 42-43 0.0 0.0 0.0 0.08575085324232082 0.0 44-45 0.0 0.0 0.0 0.1493174061433447 0.0 46-47 0.0 0.0 0.0 0.19752559726962457 0.0 48-49 0.0 0.0 0.0 0.3242320819112628 0.0 50-51 0.0 0.0 0.0 0.4496587030716723 0.0 52-53 0.0 0.0 0.0 0.5951365187713311 0.0 54-55 0.0 0.0 0.0 0.8886518771331058 0.0 56-57 0.0 0.0 0.0 1.2222696245733788 0.0 58-59 0.0 0.0 0.0 1.6377986348122868 0.0 60-61 0.0 0.0 0.0 2.1429180887372015 0.0 62-63 0.0 0.0 0.0 2.8737201365187715 0.0 64-65 0.0 0.0 0.0 3.3732935153583616 0.0 66-67 0.0 0.0 0.0 4.052901023890785 0.0 68-69 0.0 0.0 0.0 4.837883959044369 0.0 70-71 0.0 0.0 0.0 5.485068259385665 0.0 72-73 0.0 0.0 0.0 6.636518771331058 0.0 74-75 0.0 0.0 0.0 7.904436860068259 0.0 76-77 0.0 0.0 0.0 8.982935153583618 0.0 78-79 0.0 0.0 0.0 9.948378839590443 0.0 80-81 0.0 0.0 0.0 11.188139931740615 0.0 82-83 0.0 0.0 0.0 12.309726962457338 0.0 84-85 0.0 0.0 0.0 13.558873720136518 0.0 86-87 0.0 0.0 0.0 15.55588737201365 0.0 88-89 0.0 0.0 0.0 18.24872013651877 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 75 2.3483153E-9 50.681686 5 AGGTAAG 60 1.4777233E-6 47.514088 2 GTAAGGT 60 1.4777233E-6 47.514088 4 ATACACC 40 5.3717254E-4 47.493813 8 AAGGTGT 75 1.3501995E-7 44.327557 6 GTCCTGG 45 9.555566E-4 42.252777 1 CTATCCC 45 9.5756794E-4 42.234745 4 GGTGTGC 95 1.9052095E-8 39.99479 8 GTATTCA 60 8.210122E-5 39.611973 1 GACTGAT 50 0.001610931 37.995052 7 AGGTGTG 90 5.645725E-7 36.939636 7 GACTATG 55 0.0025718655 34.540955 7 TATATGG 70 2.0385816E-4 33.938633 2 TAATCGG 35 2.0499337E-4 33.924156 14-15 GGTAAGG 85 1.6164917E-5 33.539352 3 GTCCAAG 75 3.048754E-4 31.689579 1 TAAAGAG 60 0.0039309007 31.676058 5 ACTGTGC 60 0.0039391275 31.662544 8 AATCGGA 30 0.00394711 31.662544 16-17 AAGGTAA 100 4.8915517E-5 28.520622 1 >>END_MODULE