FastQCFastQC Report
Thu 26 May 2016
SRR937321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141957
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.8347598216361293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.6375169945828666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.44168304486569876No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.1824496150242679No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2500.17610966701184164No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2420.17047415766746268No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2330.1641342096550364No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1940.1366611016011891No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.11059687088343653No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1490.1049613615390576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG608.2102204E-539.618992
ATAGGAG500.001603916938.0342253
CCTTATA550.002560682434.5765722
AATAGAC851.6200056E-533.5358967
TAACACT1001.2795063E-633.279954
GTATCAA11050.031.849871
GTATCTA600.003915231731.7064041
GGTATCA7550.030.8664971
CATGGGT953.448206E-530.0270234
CTACACT804.4504268E-429.7142414
TAGACGA804.468907E-429.6932419
TTATCCG650.00582149229.2364229
TACATGG6800.027.9663432
ATAGACG856.372029E-427.9465798
AAGGTAC700.00837369827.1385176
GTACATG7100.026.7941441
AATACCT908.880257E-426.4033245
CGAAATC458.920743E-426.39399512-13
CATGGGA4350.026.2305034
ACATGGG6950.025.994623
Produced by FastQC (version 0.11.5)