FastQCFastQC Report
Thu 26 May 2016
SRR937321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141957
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.7502271814704453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.7107786160597928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.4304120261769409No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5590.3937812154384778No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.17329191233965213No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.1718830350035574No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC1830.12891227625266807No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1750.12327676690828913No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1610.113414625555626No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1450.10214360686686813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACG156.610456E-494.9834445
AGGTAAG602.3350367E-855.4070052
GTAAGGT701.2823875E-954.2762534
GGTAAGG707.866038E-847.4917223
TAAGGTG707.866038E-847.4917225
GGTGTGC857.1559043E-944.698098
AAGGTAA802.2407767E-741.5698971
ATATTCG756.8877725E-637.9933743
TATTCGT801.0722542E-535.6187934
TCGTAGC851.6241234E-533.5235677
CATCAAT600.00393596131.6723061
AGGTGTG1051.8887586E-631.6611487
ATTCGTA902.401062E-531.6611465
TTCGTAG953.4736862E-529.9947726
GGTATCA12450.029.7643341
AAGGTGT1303.0098818E-729.2256766
GTGTGCA1153.8384824E-628.9080059
GTAGCTT856.385843E-427.9363069
CGTAGCT856.385843E-427.9363068
GTACACT1205.3454587E-627.7035036
Produced by FastQC (version 0.11.5)