##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937321_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 141957 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39187923103475 33.0 31.0 34.0 31.0 34.0 2 32.598399515346195 34.0 31.0 34.0 31.0 34.0 3 32.651598723557136 34.0 31.0 34.0 31.0 34.0 4 36.21976373127074 37.0 35.0 37.0 35.0 37.0 5 36.162760554252344 37.0 35.0 37.0 35.0 37.0 6 36.26325577463598 37.0 37.0 37.0 35.0 37.0 7 36.22170093760787 37.0 37.0 37.0 35.0 37.0 8 36.205195939615514 37.0 37.0 37.0 35.0 37.0 9 37.942961601048204 39.0 38.0 39.0 35.0 39.0 10-11 37.89124171404016 39.0 38.0 39.0 35.0 39.0 12-13 37.82736673781497 39.0 38.0 39.0 35.0 39.0 14-15 39.051582521467765 40.0 38.0 41.0 36.0 41.0 16-17 39.03724367237966 40.0 38.0 41.0 36.0 41.0 18-19 39.093954507350816 40.0 38.5 41.0 36.0 41.0 20-21 39.032097747909575 40.0 38.0 41.0 36.0 41.0 22-23 38.91749262100495 40.0 38.0 41.0 35.0 41.0 24-25 38.76717245363032 40.0 38.0 41.0 35.0 41.0 26-27 38.70938382749706 40.0 38.0 41.0 35.0 41.0 28-29 38.61141049754503 40.0 38.0 41.0 35.0 41.0 30-31 38.63935910169981 40.0 38.0 41.0 35.0 41.0 32-33 38.50384623512754 40.0 38.0 41.0 35.0 41.0 34-35 38.341684453743035 40.0 38.0 41.0 34.5 41.0 36-37 38.1828546672584 40.0 38.0 41.0 34.0 41.0 38-39 38.01282782814515 40.0 37.0 41.0 34.0 41.0 40-41 37.845016448642895 40.0 37.0 41.0 33.5 41.0 42-43 37.59041118085054 40.0 37.0 41.0 33.0 41.0 44-45 37.18349570644632 40.0 37.0 41.0 33.0 41.0 46-47 36.66946328817882 40.0 37.0 41.0 31.5 41.0 48-49 36.22190874701494 40.0 36.0 41.0 30.0 41.0 50-51 35.926858133096644 40.0 36.0 41.0 29.0 41.0 52-53 35.691191698894734 39.5 35.5 41.0 28.0 41.0 54-55 35.464274392949974 39.0 35.0 41.0 27.5 41.0 56-57 35.19036046126644 39.0 35.0 40.5 26.5 41.0 58-59 34.93175398183958 39.0 35.0 40.0 26.0 41.0 60-61 34.5901188388033 38.0 35.0 40.0 25.0 41.0 62-63 34.23770226195256 38.0 34.0 40.0 23.5 41.0 64-65 33.908673048881 37.5 34.0 40.0 22.5 41.0 66-67 33.49673140458026 37.0 34.0 40.0 21.0 41.0 68-69 33.07181047782075 36.5 33.0 39.0 18.5 41.0 70-71 32.624594067217544 36.0 33.0 39.0 15.0 40.0 72-73 32.17082285480815 35.5 32.5 38.5 9.0 40.0 74-75 31.723433856731262 35.0 32.0 37.5 7.0 40.0 76-77 30.417950506139185 34.0 30.5 36.0 3.5 39.0 78-79 30.997358354994823 35.0 31.5 36.5 2.0 39.0 80-81 30.805430517691978 35.0 32.0 36.0 2.0 38.5 82-83 30.47604556309305 35.0 31.0 36.0 2.0 37.0 84-85 30.11806744295808 34.5 31.0 35.0 2.0 37.0 86-87 29.780437033749656 34.0 31.0 35.0 2.0 36.5 88-89 29.441936642786196 34.0 30.5 35.0 2.0 36.0 90-91 29.028635431856124 34.0 30.0 35.0 2.0 36.0 92-93 28.720196961051585 34.0 29.5 35.0 2.0 35.0 94-95 28.3432130856527 34.0 29.0 35.0 2.0 35.0 96-97 27.901019322752667 34.0 29.0 35.0 2.0 35.0 98-99 27.516029501891417 34.0 29.0 35.0 2.0 35.0 100-101 25.796625034341382 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1102435300497504 1101 2 0.12488871001923485 1101 3 0.15769111865743213 1101 4 0.07985121716576771 1101 5 0.011039540603952958 1101 6 -0.09418371356873934 1101 7 0.15002232957621686 1101 8 0.10773045738197595 1101 9 0.21010095914986238 1101 10-11 -0.034108287409907234 1101 12-13 0.08083443142036373 1101 14-15 0.06149487038705814 1101 16-17 0.1498755028263261 1101 18-19 0.10099609527147635 1101 20-21 -0.11371611361210654 1101 22-23 -0.056294953974401096 1101 24-25 -0.05175803667761869 1101 26-27 -1.7636934715170582E-4 1101 28-29 -0.08399539990999472 1101 30-31 0.3369150294198917 1101 32-33 0.35381927897172716 1101 34-35 0.4420015746809369 1101 36-37 0.5042866847176839 1101 38-39 0.3176746902280172 1101 40-41 0.4039785050044742 1101 42-43 0.42677905402963034 1101 44-45 0.7318547160129114 1101 46-47 1.2855191390041014 1101 48-49 1.4379985558109496 1101 50-51 1.4091889124487906 1101 52-53 1.399477932188816 1101 54-55 1.166101140312037 1101 56-57 1.423780605705609 1101 58-59 1.257306118887037 1101 60-61 1.3339678125097052 1101 62-63 1.0663667745542043 1101 64-65 0.9460459315080385 1101 66-67 1.3989878242706126 1101 68-69 1.474575884896261 1101 70-71 1.3301306039748866 1101 72-73 1.2355774863465179 1101 74-75 1.3390924048997555 1101 76-77 1.4358867374639779 1101 78-79 1.5533562124203932 1101 80-81 1.4626409202688713 1101 82-83 1.2797865321589974 1101 84-85 1.4916223914837836 1101 86-87 1.770896677493937 1101 88-89 1.6326105973507623 1101 90-91 1.6026905603325083 1101 92-93 1.852453054598275 1101 94-95 1.752462263378849 1101 96-97 1.653888283480537 1101 98-99 1.7582381766956487 1101 100-101 1.4958813571687024 1102 1 -0.17400925420303537 1102 2 -0.0552459701154433 1102 3 -0.04316358219726624 1102 4 -0.10947797216341826 1102 5 -0.18049115092673418 1102 6 -0.02593714532216751 1102 7 -0.1405759610220727 1102 8 -0.07162722197570304 1102 9 -0.12452737547847192 1102 10-11 -0.053468556770177145 1102 12-13 -0.12183327124733268 1102 14-15 0.034235093127279015 1102 16-17 -0.22166036870954997 1102 18-19 -0.225279481004101 1102 20-21 -0.05518205507804197 1102 22-23 0.05598165830220836 1102 24-25 0.09528936036976887 1102 26-27 -0.05637942555021169 1102 28-29 -0.42529299120758424 1102 30-31 -0.1502644577595973 1102 32-33 -0.1582889831365364 1102 34-35 -0.2377446050652452 1102 36-37 0.07182100225200116 1102 38-39 -0.09834447579114425 1102 40-41 0.0630694140953878 1102 42-43 -0.3212136939631165 1102 44-45 0.13246336662157177 1102 46-47 0.20102030450526343 1102 48-49 0.01336763118003148 1102 50-51 -0.4155974123375401 1102 52-53 -0.1850856523747737 1102 54-55 -0.45951398530309007 1102 56-57 -0.32997489804989755 1102 58-59 -0.1186327570518344 1102 60-61 -0.3040793255374368 1102 62-63 0.1578586160460489 1102 64-65 0.45951394497605236 1102 66-67 0.12360579888858325 1102 68-69 -0.4266155162951364 1102 70-71 0.007545781390064121 1102 72-73 0.14764689841592826 1102 74-75 -0.09292002711267955 1102 76-77 -0.16467011309287471 1102 78-79 0.0011676602318395624 1102 80-81 0.1974894051173557 1102 82-83 0.5063470087194766 1102 84-85 0.30501270487409826 1102 86-87 0.5144216710189298 1102 88-89 0.2358222005623638 1102 90-91 0.484262691904636 1102 92-93 0.5002774524226759 1102 94-95 0.6845394454560285 1102 96-97 0.513639643231901 1102 98-99 0.2178988863563589 1102 100-101 0.4372825485698968 1103 1 -0.008043162007432159 1103 2 0.1988523905402957 1103 3 0.07936689279222264 1103 4 0.16402630134085427 1103 5 -0.2161503915040086 1103 6 -0.17593013957745995 1103 7 0.046197192964193334 1103 8 0.06197232965663346 1103 9 -0.2558150313562919 1103 10-11 -0.19782192653338626 1103 12-13 -0.17290515018806474 1103 14-15 0.07336217733632822 1103 16-17 -0.1638629748465874 1103 18-19 -0.05493479836433579 1103 20-21 0.12983966273055358 1103 22-23 -0.0318703803695044 1103 24-25 -0.15856379922109198 1103 26-27 -0.019108863689488942 1103 28-29 0.0015320052895404501 1103 30-31 0.4088640275983977 1103 32-33 0.09577434879565061 1103 34-35 0.3240460632828075 1103 36-37 0.24798045300161675 1103 38-39 0.3047310461368298 1103 40-41 0.2675320734760831 1103 42-43 0.42699984113566103 1103 44-45 0.3439317707129206 1103 46-47 0.5689269879857193 1103 48-49 0.7466589300451005 1103 50-51 1.012103302248427 1103 52-53 0.8942894351970381 1103 54-55 1.0906471468252548 1103 56-57 0.7771779672996928 1103 58-59 0.8044441660250925 1103 60-61 0.8452831516938986 1103 62-63 0.6972659300435211 1103 64-65 0.5150695005316095 1103 66-67 0.7300580897671054 1103 68-69 0.9764249769092856 1103 70-71 0.36007485523061433 1103 72-73 0.37638446321906827 1103 74-75 0.576548937438254 1103 76-77 0.35140807101809557 1103 78-79 0.7498015400132623 1103 80-81 0.9629721009279208 1103 82-83 0.859821858095966 1103 84-85 0.9400134054485676 1103 86-87 0.8156336648538769 1103 88-89 0.5932552957003807 1103 90-91 0.27727796442810515 1103 92-93 0.4756522597646935 1103 94-95 0.7671722043182996 1103 96-97 1.3046582785128287 1103 98-99 1.006430482265003 1103 100-101 1.2260243168198635 1104 1 0.25817209102179106 1104 2 0.30707287046426757 1104 3 0.37266696406805977 1104 4 -0.00988674213741092 1104 5 0.07406106014721558 1104 6 -0.027795190658473246 1104 7 0.02683392377911531 1104 8 0.05341922915770425 1104 9 -4.0994701321750426E-4 1104 10-11 -0.30829947464457774 1104 12-13 0.05737520143939889 1104 14-15 -0.02773071883853362 1104 16-17 0.2248279256048349 1104 18-19 -0.10292719560833774 1104 20-21 -0.5174637347510256 1104 22-23 -0.02456460702666874 1104 24-25 0.18243168664253773 1104 26-27 0.16389184906888232 1104 28-29 -0.31148771435883305 1104 30-31 -0.09665984980716047 1104 32-33 0.29303022905224196 1104 34-35 0.08444045625024899 1104 36-37 0.22445942662955076 1104 38-39 -0.41764956683537235 1104 40-41 -0.1820996680302187 1104 42-43 -0.27857155349489204 1104 44-45 0.5799735858709241 1104 46-47 0.27432019302254673 1104 48-49 0.32524054087902243 1104 50-51 0.24131451631352974 1104 52-53 0.5205070640875178 1104 54-55 -0.6471765642700191 1104 56-57 0.36716371213219645 1104 58-59 0.25734271674972575 1104 60-61 0.09326366963164645 1104 62-63 -0.18857393690824154 1104 64-65 0.14912747154610173 1104 66-67 0.05365039197666022 1104 68-69 0.2561232425305704 1104 70-71 0.15925518309946796 1104 72-73 0.21435072598932337 1104 74-75 -0.48106569786704156 1104 76-77 -0.2912030004953259 1104 78-79 -0.372020745565262 1104 80-81 -0.2964719475396471 1104 82-83 0.015915942201448985 1104 84-85 -0.3002455634276515 1104 86-87 -0.4410781431765365 1104 88-89 -0.5217957853164918 1104 90-91 -0.9096429193922795 1104 92-93 -1.0339105817653618 1104 94-95 -0.18762703540610204 1104 96-97 -0.41594043852209595 1104 98-99 -0.3419301734727007 1104 100-101 0.08258169386904157 1105 1 0.13518780851515544 1105 2 0.34616248058878085 1105 3 0.23906827045711765 1105 4 0.309566207444135 1105 5 0.31421211138216165 1105 6 0.2686938887454886 1105 7 0.05612561596119292 1105 8 0.24407038245429646 1105 9 0.4100819901168151 1105 10-11 0.19027166232215365 1105 12-13 0.28318659151900505 1105 14-15 -0.0488574794019101 1105 16-17 0.5289716284013153 1105 18-19 0.4970932167207067 1105 20-21 0.33587368104812043 1105 22-23 0.15276750156692742 1105 24-25 -0.06562914209802528 1105 26-27 0.26335092234915436 1105 28-29 0.3706923223833627 1105 30-31 -0.03710687981328675 1105 32-33 0.1561145319712054 1105 34-35 0.4197129608430288 1105 36-37 -0.5517193853728912 1105 38-39 -0.9607195811240032 1105 40-41 -1.2064924012410785 1105 42-43 -0.8324090713556771 1105 44-45 -1.3957357425634527 1105 46-47 -0.8734650692758805 1105 48-49 -0.4469068361366908 1105 50-51 -0.667493403670143 1105 52-53 -0.7337795250123023 1105 54-55 -0.5210283707611367 1105 56-57 -0.4729567206091829 1105 58-59 -0.23311451942091566 1105 60-61 -0.354940045412242 1105 62-63 -0.022621701758211543 1105 64-65 0.18408358503864974 1105 66-67 0.1419337151038249 1105 68-69 0.41273683714171483 1105 70-71 0.8740439154938358 1105 72-73 0.029312963180583296 1105 74-75 -0.12078809019200776 1105 76-77 -0.8186065522405812 1105 78-79 -0.1467196637118242 1105 80-81 -0.32402859175275367 1105 82-83 -0.24281032846603168 1105 84-85 -0.6854772433623282 1105 86-87 -0.16112305030043927 1105 88-89 -0.14891356445509274 1105 90-91 -0.8649909496025252 1105 92-93 -0.4975256052395416 1105 94-95 -0.6647141960511327 1105 96-97 -0.060153543237344564 1105 98-99 -0.897094492721525 1105 100-101 0.38891972203805736 1106 1 0.15016656997278943 1106 2 0.0161826013131261 1106 3 0.11007207103838113 1106 4 0.21164534895989817 1106 5 0.27799480755922445 1106 6 0.3348687154836938 1106 7 0.3246255041793873 1106 8 0.28185263150415096 1106 9 -0.007311258262355125 1106 10-11 0.33938858608696165 1106 12-13 -0.22195537136943955 1106 14-15 -0.16265135375916628 1106 16-17 0.38700873995956186 1106 18-19 0.4001783944537678 1106 20-21 0.33553833564234736 1106 22-23 0.14572524804579956 1106 24-25 0.5211135361939512 1106 26-27 0.5498476806768977 1106 28-29 0.6995543836397928 1106 30-31 0.3131055056103733 1106 32-33 0.15276107791352445 1106 34-35 -0.6760840434046784 1106 36-37 -0.349729669298668 1106 38-39 -0.367269994716672 1106 40-41 -0.925025823999853 1106 42-43 -0.5251208978703232 1106 44-45 -0.28786630370809974 1106 46-47 -0.4323740788891115 1106 48-49 -0.7228961050837057 1106 50-51 -0.7098587065988298 1106 52-53 -0.9689684362575477 1106 54-55 -0.7879633137524209 1106 56-57 -1.3876672057422041 1106 58-59 -1.124127262546338 1106 60-61 -0.32788884934695517 1106 62-63 -0.4877457795583453 1106 64-65 -0.9214384359763272 1106 66-67 -1.3162598909771006 1106 68-69 -1.0352846249642482 1106 70-71 -1.2018559280116428 1106 72-73 -0.8346485838795523 1106 74-75 -0.6356550698477186 1106 76-77 -0.7620449604677226 1106 78-79 -0.1575624984983186 1106 80-81 -1.1318756742477234 1106 82-83 -1.2125174601449942 1106 84-85 -0.696208296346903 1106 86-87 -0.2050532984560398 1106 88-89 -0.2666198018796351 1106 90-91 -0.020926563284618283 1106 92-93 0.3986291007743219 1106 94-95 -0.19366568274909568 1106 96-97 0.06370069329294736 1106 98-99 -0.30621588775841246 1106 100-101 0.41988328117063034 1107 1 0.31964941687499504 1107 2 0.3318508040781083 1107 3 0.1379778570086856 1107 4 -0.1370489674763533 1107 5 -0.03767339520575774 1107 6 0.08627680560145734 1107 7 0.4134631897912655 1107 8 0.015083558283464527 1107 9 -0.024720372445656835 1107 10-11 0.39904040380330486 1107 12-13 0.7390021299106451 1107 14-15 0.13355960374211406 1107 16-17 0.16709066520277105 1107 18-19 0.4477974420728188 1107 20-21 0.6550467841830496 1107 22-23 0.3715316996587035 1107 24-25 0.831917427642999 1107 26-27 0.819688950518163 1107 28-29 0.8010648803115785 1107 30-31 0.5940834882012567 1107 32-33 0.46881330525607723 1107 34-35 0.6398401757453485 1107 36-37 0.5330148263490457 1107 38-39 0.5775584743053486 1107 40-41 0.6777647545971774 1107 42-43 0.9029384979310109 1107 44-45 0.9019698458054677 1107 46-47 0.4392337040089913 1107 48-49 -0.4287332786628184 1107 50-51 0.02298819560291321 1107 52-53 0.11026863491499483 1107 54-55 -0.04794795276931296 1107 56-57 -0.6466277792189032 1107 58-59 -0.7788891673082432 1107 60-61 -0.7494198273295467 1107 62-63 -0.5150115245660274 1107 64-65 -0.43654340269419833 1107 66-67 -0.23971099184755218 1107 68-69 0.09413165767784193 1107 70-71 0.5382874411639804 1107 72-73 0.04067143660175532 1107 74-75 -0.22346868991940383 1107 76-77 -0.15515811970991322 1107 78-79 -0.0068968558971924665 1107 80-81 0.5075252474112624 1107 82-83 0.3069397851187041 1107 84-85 0.37483625050409586 1107 86-87 0.37635991198942875 1107 88-89 0.07439402300515496 1107 90-91 0.24405039524073047 1107 92-93 0.8263044719980819 1107 94-95 1.0411013423405073 1107 96-97 0.8193330537640193 1107 98-99 1.155381654484291 1107 100-101 2.7519365325787213 1108 1 0.0708014906077139 1108 2 0.0697540298845567 1108 3 0.21027048373679946 1108 4 0.17890725372180327 1108 5 0.24228052184493976 1108 6 -0.10064715753217968 1108 7 0.14704613910002706 1108 8 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2316 90-91 0.7269725963792482 2316 92-93 0.7467266731365996 2316 94-95 -0.04333888536347885 2316 96-97 0.10787669702777336 2316 98-99 0.24753061084102512 2316 100-101 -0.9027124237780164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 2.0 5 4.0 6 4.0 7 12.0 8 35.0 9 58.0 10 119.0 11 168.0 12 326.0 13 458.0 14 648.0 15 963.0 16 1491.0 17 2308.0 18 2029.0 19 1065.0 20 849.0 21 812.0 22 758.0 23 771.0 24 866.0 25 936.0 26 1089.0 27 1272.0 28 1488.0 29 1775.0 30 2204.0 31 2764.0 32 3678.0 33 5092.0 34 7464.0 35 11214.0 36 18801.0 37 32649.0 38 33419.0 39 4364.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.992472299754716 21.09306718542953 16.0844681270969 19.829992387718853 2 15.556823545158041 22.21729115154589 40.33474925505611 21.89113604823996 3 19.329797051219735 26.748945103094602 31.765957296927944 22.155300548757722 4 12.205808801256719 19.12057876680967 38.930802989637705 29.742809442295908 5 12.298598733294352 39.32494029293449 34.56669226379603 13.809768709975131 6 28.817177032481666 39.306973238375 17.532774009030906 14.343075720112427 7 26.3474150623076 35.63191670717189 21.046514085251168 16.974154145269342 8 26.150172235254338 35.59669477376952 19.52069993026057 18.73243306071557 9 26.63905267087921 17.252407419148053 21.32688067513402 34.78165923483872 10-11 24.320392795001304 28.49700965785414 27.203660263319172 19.978937283825385 12-13 25.81964325969342 26.330027051397654 28.027431807935077 19.82289788097385 14-15 22.462435808026374 27.320949301549057 27.681621899589317 22.534992990835253 16-17 20.231478546320364 30.827292771754827 28.228266305994072 20.71296237593074 18-19 19.98245947716562 28.43678015173609 32.29745627196968 19.28330409912861 20-21 21.20430834689378 27.06946469705615 32.49152912501673 19.23469783103334 22-23 20.895427760303477 27.29747912183129 32.081406366124355 19.725686751740877 24-25 20.724937833287544 27.484731291870073 32.490120247680636 19.300210627161746 26-27 20.526999848543017 27.511984473898327 32.507951491499036 19.453064186059617 28-29 19.830716829339597 28.357925065955627 32.610786078343665 19.200572026361115 30-31 20.286072543094036 27.915847756715063 32.29745627196968 19.50062342822122 32-33 20.191325542241664 28.21452975196714 31.76067400691759 19.833470698873604 34-35 20.55728143029227 28.046168910303827 32.25061110054453 19.145938558859374 36-37 21.048979620589332 27.681621899589317 31.856829885106052 19.412568594715303 38-39 20.321646695830427 28.029614601604713 32.37564896412294 19.27308973844192 40-41 20.328691082510904 28.544559267947335 31.727565389519363 19.399184260022402 42-43 20.396013794609676 28.827070688568018 31.017088266479732 19.759827250342575 44-45 20.79996055143459 28.417408088364787 30.87519460118205 19.907436759018577 46-47 20.954584839070986 28.998569989503864 30.19717238318646 19.849672788238692 48-49 21.098290327352647 29.426164261008616 29.551554343921044 19.92399106771769 50-51 20.830251414160625 29.595229541339986 29.66743450481484 19.907084539684554 52-53 20.804539402776896 29.834386469142064 29.13523109110505 20.225843036975984 54-55 20.624203103756773 29.750910486978448 29.078875997661264 20.546010411603515 56-57 21.157110956134602 29.49696034714738 29.29196869474559 20.053960001972428 58-59 21.057785103939924 29.013010982198832 29.43884415703347 20.49035975682777 60-61 20.59426446036476 29.365934754890567 29.952732165374023 20.087068619370655 62-63 20.4283691540396 29.098600280366586 30.267616249991196 20.205414315602614 64-65 20.342779855871846 29.215889318596478 30.036912586205684 20.404418239325995 66-67 20.177941207548763 28.914741788006225 29.780144691702414 21.12717231274259 68-69 20.280789253083682 28.823869199828117 29.69455539353466 21.20078615355354 70-71 20.420972548025105 29.114097931063633 29.717801869580224 20.747127651331038 72-73 20.324607624897855 28.557088111809293 29.723998985601174 21.39430527769168 74-75 20.064174362659116 28.851342307881968 29.146854329127837 21.937629000331086 76-77 19.974710651817098 28.370562916939633 29.62094155272371 22.03378487851955 78-79 19.950055298435444 27.927470994737842 29.513162436512463 22.60931127031425 80-81 19.88947357298337 28.234254034672475 29.620237114055666 22.256035278288497 82-83 19.948294201765325 28.655508358164795 29.296899765421923 22.099297674647957 84-85 19.651863957846093 28.94946321395362 28.94911099213841 22.44956183606188 86-87 19.339659192572398 28.62803525011095 28.321604429510344 23.710701127806306 88-89 19.49111350620258 27.950717470783403 28.803440478454746 23.754728544559267 90-91 19.61815523891717 27.78272892192261 29.151593074660514 23.44752276449971 92-93 19.572614049423763 27.56945814195948 28.70854349234974 24.14938431626701 94-95 19.236811147037482 28.34414646688786 28.44769895109082 23.971343434983833 96-97 19.53584536162359 29.228216995287305 27.966919560148497 23.269018082940608 98-99 19.298097311157605 29.818184379776973 26.651380347570036 24.232337961495382 100-101 18.957372683432023 30.02582086029611 25.584844247020737 25.431962209251125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 93.0 1 62.5 2 35.5 3 40.5 4 45.0 5 52.0 6 71.0 7 127.5 8 193.5 9 248.0 10 274.5 11 278.0 12 307.5 13 348.0 14 374.0 15 394.0 16 420.5 17 423.0 18 434.0 19 452.0 20 487.5 21 561.5 22 619.0 23 712.0 24 838.5 25 996.0 26 1178.0 27 1417.0 28 1694.5 29 1990.5 30 2387.5 31 2726.0 32 3008.5 33 3334.0 34 3638.0 35 3920.0 36 4364.0 37 4645.5 38 4721.5 39 4893.5 40 4944.0 41 5074.0 42 5117.5 43 5119.5 44 5227.0 45 5186.5 46 5127.0 47 5413.5 48 5993.0 49 5641.5 50 4769.0 51 4293.0 52 3857.5 53 3599.0 54 3439.5 55 3141.0 56 2696.5 57 2273.0 58 2017.5 59 1652.5 60 1226.0 61 959.5 62 746.0 63 516.5 64 341.0 65 244.5 66 161.0 67 127.5 68 89.5 69 48.0 70 41.0 71 32.5 72 22.0 73 16.0 74 9.0 75 8.0 76 6.5 77 3.0 78 2.5 79 7.0 80 5.5 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05705953211183668 2 0.0 3 0.0 4 0.0 5 0.009862141352663131 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035221933402368325 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0017610966701184162 24-25 0.0 26-27 0.0017610966701184162 28-29 0.0038744126742605153 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012327676690828914 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.044386680473665E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 7.044386680473665E-4 86-87 0.0 88-89 0.0 90-91 0.010214360686686814 92-93 7.044386680473665E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.012327676690828914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 141957.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.64259429563495 #Duplication Level Percentage of deduplicated Percentage of total 1 88.69484804314324 64.43023862511048 2 6.71798849037529 9.760242247781546 3 1.8061646805215394 3.9361346435469393 4 0.8210020462401636 2.3855887424364135 5 0.497999241610746 1.8087978433931655 6 0.32499944599373654 1.416528174097748 7 0.2049996488633504 1.0424194426189592 8 0.1359997670001728 0.790350071879555 9 0.11199980811590252 0.7322360959937222 >10 0.6319989172249488 8.150485116114117 >50 0.03399994174944326 1.6482576406829659 >100 0.01399997601447664 1.5421996347157059 >500 0.0019999965734966625 0.8499168971348922 >1k 0.0019999965734966625 1.506604824493817 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1065 0.7502271814704453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 0.7107786160597928 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 611 0.4304120261769409 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 559 0.3937812154384778 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 246 0.17329191233965213 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 244 0.1718830350035574 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 183 0.12891227625266807 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 175 0.12327676690828913 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 161 0.113414625555626 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 145 0.10214360686686813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.5221933402368325E-4 0.0 0.0 0.0 0.0 14-15 7.044386680473665E-4 0.0 0.0 0.0 0.0 16-17 7.044386680473665E-4 0.0 0.0 0.0 0.0 18-19 7.044386680473665E-4 0.0 0.0 0.0 0.0 20-21 7.044386680473665E-4 0.0 0.0 0.0 0.0 22-23 7.044386680473665E-4 0.0 0.0 7.044386680473665E-4 0.0 24-25 7.044386680473665E-4 0.0 0.0 0.001408877336094733 0.0 26-27 7.044386680473665E-4 0.0 0.0 0.0024655353381657827 0.0 28-29 7.044386680473665E-4 0.0 0.0 0.005635509344378932 0.0 30-31 7.044386680473665E-4 0.0 0.0 0.012327676690828914 7.044386680473665E-4 32-33 7.044386680473665E-4 0.0 0.0 0.01866762470325521 0.001408877336094733 34-35 7.044386680473665E-4 0.0 0.0 0.029938643392013076 0.001408877336094733 36-37 7.044386680473665E-4 0.0 0.0 0.041561881414794624 0.001408877336094733 38-39 7.044386680473665E-4 0.0 0.0 0.051776242101481434 0.001408877336094733 40-41 7.044386680473665E-4 0.0 0.0 0.06551279612840508 0.001408877336094733 42-43 0.0010566580020710497 0.0 0.0 0.11024465154941285 0.001408877336094733 44-45 0.001408877336094733 0.0 0.0 0.16025979698077586 0.001408877336094733 46-47 0.001408877336094733 0.0 0.0 0.21309269708432835 0.001408877336094733 48-49 0.001408877336094733 0.0 0.0 0.34094831533492537 0.001408877336094733 50-51 0.001408877336094733 0.0 0.0 0.4592940115668829 0.001408877336094733 52-53 0.001408877336094733 0.0 0.0 0.6001817451763563 0.001408877336094733 54-55 0.001408877336094733 0.0 0.0 0.8756172643828766 0.001408877336094733 56-57 0.001408877336094733 0.0 0.0 1.1771170143071492 0.001408877336094733 58-59 0.001408877336094733 0.0 0.0 1.5860436611086457 0.001408877336094733 60-61 0.001408877336094733 0.0 0.0 2.0664708327169494 0.001408877336094733 62-63 0.001408877336094733 0.0 0.0 2.774079474770529 0.001408877336094733 64-65 0.001408877336094733 0.0 0.0 3.234430144339483 0.001408877336094733 66-67 0.001408877336094733 0.0 0.0 3.8624372169037104 0.001408877336094733 68-69 0.001408877336094733 0.0 0.0 4.647181893108476 0.001408877336094733 70-71 0.001408877336094733 0.0 0.0 5.270610114330395 0.001408877336094733 72-73 0.001408877336094733 0.0 0.0 6.298738350345527 0.001408877336094733 74-75 0.001408877336094733 0.0 0.0 7.493818550687884 0.001408877336094733 76-77 0.001408877336094733 0.0 0.0 8.50504025866988 0.001408877336094733 78-79 0.001408877336094733 0.0 0.0 9.376783110378495 0.001408877336094733 80-81 0.001408877336094733 0.0 0.0 10.475355213198362 0.001408877336094733 82-83 0.001408877336094733 0.0 0.0 11.59012940538332 0.001408877336094733 84-85 0.001408877336094733 0.0 0.0 12.800707256422719 0.001408877336094733 86-87 0.001408877336094733 0.0 0.0 14.737913593552978 0.001408877336094733 88-89 0.001408877336094733 0.0 0.0 17.36406094803356 0.001408877336094733 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAAACG 15 6.610456E-4 94.983444 5 AGGTAAG 60 2.3350367E-8 55.407005 2 GTAAGGT 70 1.2823875E-9 54.276253 4 GGTAAGG 70 7.866038E-8 47.491722 3 TAAGGTG 70 7.866038E-8 47.491722 5 GGTGTGC 85 7.1559043E-9 44.69809 8 AAGGTAA 80 2.2407767E-7 41.569897 1 ATATTCG 75 6.8877725E-6 37.993374 3 TATTCGT 80 1.0722542E-5 35.618793 4 TCGTAGC 85 1.6241234E-5 33.523567 7 CATCAAT 60 0.003935961 31.672306 1 AGGTGTG 105 1.8887586E-6 31.661148 7 ATTCGTA 90 2.401062E-5 31.661146 5 TTCGTAG 95 3.4736862E-5 29.994772 6 GGTATCA 1245 0.0 29.764334 1 AAGGTGT 130 3.0098818E-7 29.225676 6 GTGTGCA 115 3.8384824E-6 28.908005 9 GTAGCTT 85 6.385843E-4 27.936306 9 CGTAGCT 85 6.385843E-4 27.936306 8 GTACACT 120 5.3454587E-6 27.703503 6 >>END_MODULE