FastQCFastQC Report
Thu 26 May 2016
SRR937320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259713
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.6526434949347933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.5205746343078705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.32304890398247293No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4760.18327923515572958No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4590.1767335481858821No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.14439015374663572No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3640.1401547092367344No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3240.12475309283709327No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3020.11628220381729062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.036.6028181
GTATAGG851.625994E-533.5334971
CCAGTAC953.4822224E-529.9978683
GTAAGGT804.48095E-429.6911184
GTATCAA18550.028.682741
CATGGGA8600.028.1720354
GTACATG13900.027.341461
TACATGG14300.026.5715392
GAGTATT908.9224376E-426.3921051
GTATCAC908.9224376E-426.3921051
ACATGGG13700.026.3484943
ATGGGAG3150.025.6380445
GGTCTGC950.001224500724.9982228
CAACGCA22000.024.1847635
ATCAACG21800.024.1840613
TCAACGC22050.024.1299254
AACGCAG22350.024.0185856
TATCAAC22150.023.5874882
GAGTACT12350.023.45987112-13
GTATAAA1703.1301006E-622.3556631
Produced by FastQC (version 0.11.5)