##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937320_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 259713 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.998017041888545 33.0 31.0 34.0 31.0 34.0 2 32.10992133624424 33.0 31.0 34.0 30.0 34.0 3 32.14253040856638 34.0 31.0 34.0 30.0 34.0 4 35.738757782629285 37.0 35.0 37.0 35.0 37.0 5 35.749111519253944 37.0 35.0 37.0 35.0 37.0 6 35.80293246776249 37.0 35.0 37.0 35.0 37.0 7 35.67108693057337 37.0 35.0 37.0 35.0 37.0 8 35.698070562505535 37.0 35.0 37.0 35.0 37.0 9 37.372638258385216 39.0 37.0 39.0 35.0 39.0 10-11 37.281880383346234 39.0 37.0 39.0 35.0 39.0 12-13 37.15586628316642 39.0 37.0 39.0 34.0 39.0 14-15 38.3561392768171 40.0 38.0 41.0 34.0 41.0 16-17 38.31405436000509 40.0 38.0 41.0 34.0 41.0 18-19 38.31932171281376 40.0 38.0 41.0 34.0 41.0 20-21 38.23578719586621 40.0 38.0 41.0 34.0 41.0 22-23 38.07469013873006 40.0 38.0 41.0 33.5 41.0 24-25 38.02310820020561 40.0 38.0 41.0 33.5 41.0 26-27 38.01973524621409 40.0 38.0 41.0 33.5 41.0 28-29 37.92983408608734 40.0 38.0 41.0 33.0 41.0 30-31 37.69754883275 40.0 37.5 41.0 33.0 41.0 32-33 37.41894321809074 40.0 36.5 41.0 33.0 41.0 34-35 37.457855401924434 40.0 37.0 41.0 33.0 41.0 36-37 37.360374721327005 40.0 37.0 41.0 33.0 41.0 38-39 37.228240403830384 40.0 37.0 41.0 32.5 41.0 40-41 37.01213262331882 40.0 36.5 41.0 31.5 41.0 42-43 36.7314400896374 40.0 36.0 41.0 31.0 41.0 44-45 36.45838290728612 40.0 36.0 41.0 30.0 41.0 46-47 36.04397931562917 39.0 35.5 41.0 29.5 41.0 48-49 35.64958627407947 39.0 35.0 41.0 27.5 41.0 50-51 34.64858516901349 38.0 34.0 40.0 25.5 40.5 52-53 34.74592916026537 38.5 35.0 40.0 26.0 40.5 54-55 35.12813182243477 39.0 35.0 40.0 26.0 41.0 56-57 34.91820394050356 39.0 35.0 40.0 24.5 41.0 58-59 34.67887244766338 38.5 35.0 40.0 24.0 41.0 60-61 34.35982603874277 38.0 34.0 40.0 23.0 41.0 62-63 34.064694489686694 38.0 34.0 40.0 22.0 41.0 64-65 33.65044106378964 37.5 34.0 40.0 20.5 41.0 66-67 33.293762730398555 37.0 33.0 40.0 18.5 41.0 68-69 32.833254400049285 36.5 33.0 39.0 16.0 41.0 70-71 32.34901410403022 36.0 32.0 39.0 10.0 40.5 72-73 31.90294286385356 35.5 32.0 38.5 7.5 40.0 74-75 31.40373989750224 35.0 31.0 37.5 4.5 40.0 76-77 30.93357282846835 35.0 31.0 37.0 2.0 39.0 78-79 30.433420737506403 35.0 30.5 36.5 2.0 39.0 80-81 29.93257172340237 34.0 30.0 36.0 2.0 38.5 82-83 29.43086791958816 34.0 29.0 35.5 2.0 37.0 84-85 28.906261912187684 34.0 29.0 35.0 2.0 37.0 86-87 28.494264823093182 34.0 29.0 35.0 2.0 36.0 88-89 28.025135437964213 34.0 27.5 35.0 2.0 36.0 90-91 27.521427498816003 33.5 26.5 35.0 2.0 35.5 92-93 27.024760023564472 33.0 26.0 35.0 2.0 35.0 94-95 26.488643233107315 33.0 25.0 35.0 2.0 35.0 96-97 25.80220281618556 32.5 23.5 35.0 2.0 35.0 98-99 24.91887391081694 32.0 13.0 34.5 2.0 35.0 100-101 22.43376342347129 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.32257018789475467 1101 2 -0.1966275478453383 1101 3 -0.32998378426997377 1101 4 -0.37740153077406546 1101 5 -0.5653313379027338 1101 6 -0.3648284209230752 1101 7 -0.3252255778886237 1101 8 -0.495759530355123 1101 9 -0.5704101223250859 1101 10-11 -0.618024834810889 1101 12-13 -0.45648823879659517 1101 14-15 -0.843034251174636 1101 16-17 -0.6935440936995079 1101 18-19 -0.6073734476212493 1101 20-21 -0.6231245320516408 1101 22-23 -0.8326627935012141 1101 24-25 -0.7645488054849423 1101 26-27 -0.9141203702111227 1101 28-29 -1.069168801746784 1101 30-31 -0.9948945450886768 1101 32-33 -0.8051583546213337 1101 34-35 -0.5613766844208641 1101 36-37 -0.39793583761400697 1101 38-39 -0.44062641401413316 1101 40-41 -0.5358102112730307 1101 42-43 -0.2943285935857034 1101 44-45 0.20817317939425095 1101 46-47 -0.1014577485176531 1101 48-49 0.28491337495357527 1101 50-51 0.3262438194120847 1101 52-53 0.3488132227722076 1101 54-55 0.18438129854830265 1101 56-57 0.29050849172025295 1101 58-59 0.15408133221307452 1101 60-61 0.20916340232646746 1101 62-63 0.2541871328819667 1101 64-65 0.28414956951510106 1101 66-67 0.456044565359889 1101 68-69 0.4414629607631788 1101 70-71 0.5114388148199147 1101 72-73 0.7715832772331659 1101 74-75 0.5017834432755066 1101 76-77 0.7043352736484003 1101 78-79 0.625175161557646 1101 80-81 0.3754938286514644 1101 82-83 0.2137030653255998 1101 84-85 0.23110695884427557 1101 86-87 0.37936819235817154 1101 88-89 0.3192239594838817 1101 90-91 0.5196918534097286 1101 92-93 0.39179614706944577 1101 94-95 0.1365069154946994 1101 96-97 0.19958279129987844 1101 98-99 0.4955719182383156 1101 100-101 0.7789783991831598 1102 1 -0.39657683586001724 1102 2 -0.3933621672205092 1102 3 -0.21851508350526672 1102 4 -0.3234396508985711 1102 5 -0.2176485188580486 1102 6 -0.3198633899889032 1102 7 -0.4405370331397478 1102 8 -0.3458632847262564 1102 9 -0.3432649046330454 1102 10-11 -0.3897147821651501 1102 12-13 -0.3299679490529073 1102 14-15 -0.17735148977126158 1102 16-17 -0.29881154941175936 1102 18-19 -0.3763806719996836 1102 20-21 -0.2767866770771903 1102 22-23 -0.6752587662945118 1102 24-25 -0.5276525982836162 1102 26-27 -0.7885151363795089 1102 28-29 -0.8082409686760457 1102 30-31 -0.7942379144158096 1102 32-33 -0.7256037298663145 1102 34-35 -0.4487815762473275 1102 36-37 -0.6830706222276177 1102 38-39 -0.4223949456976328 1102 40-41 -0.5015880808499489 1102 42-43 -0.5755077427998572 1102 44-45 -0.13233089392550568 1102 46-47 -0.018812108122617133 1102 48-49 -0.1429052854622057 1102 50-51 -0.1637554120370197 1102 52-53 -0.12471980570260399 1102 54-55 0.11379470207594977 1102 56-57 0.2675024009543989 1102 58-59 0.40289488176539123 1102 60-61 0.39432892897596616 1102 62-63 0.14564132724849088 1102 64-65 -0.10579154027239213 1102 66-67 -0.021456107122141077 1102 68-69 0.12396978165238792 1102 70-71 0.44712311332508037 1102 72-73 -0.04639264790997899 1102 74-75 -0.04322241692508655 1102 76-77 0.0066423673731783595 1102 78-79 0.20202784956440922 1102 80-81 0.19084707385762556 1102 82-83 0.26033008757514864 1102 84-85 0.1320503550706924 1102 86-87 0.10039382353059878 1102 88-89 -0.18487818093036523 1102 90-91 -0.20795772580865446 1102 92-93 -0.05273849536225228 1102 94-95 -0.1634945255382334 1102 96-97 -0.08387078822592642 1102 98-99 0.38728309681112094 1102 100-101 0.17764976681948141 1103 1 0.025934785687699247 1103 2 -0.1756632336684163 1103 3 -0.17524190877187706 1103 4 -0.15048367605579926 1103 5 -0.2796528940289491 1103 6 -0.385593496633696 1103 7 -0.08402896641839419 1103 8 -0.3946054230910363 1103 9 -0.19259495658819503 1103 10-11 -0.14926837800874182 1103 12-13 -0.34427270705126745 1103 14-15 -0.5166152305423921 1103 16-17 -0.18625344167318048 1103 18-19 -0.3804482137005252 1103 20-21 -0.44379515397082514 1103 22-23 -0.6227908964558466 1103 24-25 -0.3385289997055452 1103 26-27 -0.43111836306805174 1103 28-29 -0.28479278710645417 1103 30-31 -0.4174092297015548 1103 32-33 -0.028379228909244603 1103 34-35 -0.020647176466084716 1103 36-37 0.07484228452655373 1103 38-39 -0.038183160081011636 1103 40-41 -0.047886276091951174 1103 42-43 0.16615208766226885 1103 44-45 0.2779951930310176 1103 46-47 0.5108057480436372 1103 48-49 0.3107110667390458 1103 50-51 0.4974081099755523 1103 52-53 0.7891781981256756 1103 54-55 0.6324575404680814 1103 56-57 0.6503811266858719 1103 58-59 0.7339722676007767 1103 60-61 0.7713626998263905 1103 62-63 1.2060562848640188 1103 64-65 0.9084448556805711 1103 66-67 1.3142015357545347 1103 68-69 1.5758087206734395 1103 70-71 1.6530125035356313 1103 72-73 1.9270999689891148 1103 74-75 1.388911162511608 1103 76-77 1.5627505161289896 1103 78-79 1.5983738982381865 1103 80-81 1.3202051542513935 1103 82-83 1.8837235795084268 1103 84-85 2.0046576834819554 1103 86-87 1.902280615983429 1103 88-89 2.1600767001196743 1103 90-91 1.85561897640628 1103 92-93 1.7047813296309116 1103 94-95 1.3071952474997772 1103 96-97 0.8614566659715059 1103 98-99 1.030364857817407 1103 100-101 0.7605045658350669 1104 1 -0.3193271987047197 1104 2 -0.49840570858480504 1104 3 -0.43694759439060604 1104 4 -0.6107400863122123 1104 5 -0.3775904637056513 1104 6 -0.2563292825868686 1104 7 -0.41150945694236185 1104 8 -0.13947721796283474 1104 9 -0.21176998445887563 1104 10-11 -0.3730420680867823 1104 12-13 -0.6281355833054434 1104 14-15 -0.48952492953904425 1104 16-17 -0.664486440558349 1104 18-19 -0.43875367635381934 1104 20-21 -0.23955881060403783 1104 22-23 -0.2916593468460391 1104 24-25 -0.5689080409541489 1104 26-27 -0.6308953976276968 1104 28-29 -0.6616879933494815 1104 30-31 -0.49109930773946076 1104 32-33 -0.26569806948895547 1104 34-35 -0.17931495795996 1104 36-37 -0.11643419261949361 1104 38-39 -0.2460705625336317 1104 40-41 -0.015612028600308747 1104 42-43 -0.11291703162424227 1104 44-45 0.04747679838218488 1104 46-47 0.17808556967128908 1104 48-49 0.31583927186725447 1104 50-51 0.6159978535653039 1104 52-53 0.616825912729908 1104 54-55 0.5411531926419855 1104 56-57 0.9028616172098438 1104 58-59 1.038598800488181 1104 60-61 0.7524385080761178 1104 62-63 0.875231312734698 1104 64-65 0.952898590351694 1104 66-67 0.966932862398906 1104 68-69 0.9062992446422307 1104 70-71 0.9136869739927143 1104 72-73 1.133705320159688 1104 74-75 1.6818320097802832 1104 76-77 1.466457316574921 1104 78-79 1.5067629729316074 1104 80-81 1.4431148532480478 1104 82-83 1.3229933676912573 1104 84-85 1.515722343459661 1104 86-87 1.2695883082911195 1104 88-89 0.9878358974440893 1104 90-91 1.0407360332624656 1104 92-93 0.8455561345361495 1104 94-95 0.949948870688182 1104 96-97 0.8795726080004904 1104 98-99 1.5579289603815134 1104 100-101 1.2070764721895841 1105 1 -0.16855796793548805 1105 2 -0.04109801627711107 1105 3 0.029975482532471176 1105 4 -0.2538170093891381 1105 5 -0.4006673867825725 1105 6 -0.3486369748945606 1105 7 -0.06497099540390394 1105 8 -0.49293875642437257 1105 9 -0.18100075368964497 1105 10-11 -0.5466959142406225 1105 12-13 -0.2002509679208302 1105 14-15 -0.43933262184673794 1105 16-17 -0.07371720978911611 1105 18-19 -0.1604844455845864 1105 20-21 -0.1951357336809636 1105 22-23 -0.19242857761526722 1105 24-25 -0.35986957941569386 1105 26-27 -0.20724155147384948 1105 28-29 -0.5489956856571752 1105 30-31 -0.0949454615856169 1105 32-33 -0.2406980694889569 1105 34-35 0.0689542728092789 1105 36-37 0.18183503814974245 1105 38-39 -0.1362628702259414 1105 40-41 -0.29484279783107326 1105 42-43 0.07727527606806461 1105 44-45 -0.1873308939255054 1105 46-47 0.1775086465943687 1105 48-49 0.4423777334057206 1105 50-51 0.18407477664221972 1105 52-53 0.4474028358068338 1105 54-55 0.5186531926419917 1105 56-57 0.24882315567138136 1105 58-59 0.2662911081804893 1105 60-61 0.23263081576842382 1105 62-63 0.0894620819654719 1105 64-65 0.1998216672747759 1105 66-67 0.10981747778352258 1105 68-69 0.12533770618069084 1105 70-71 -0.38169764139189866 1105 72-73 -0.23648698753261854 1105 74-75 -0.22605260560433038 1105 76-77 -0.5837349911173852 1105 78-79 -0.45419856552992854 1105 80-81 -0.5595774544442591 1105 82-83 -0.035660478462588685 1105 84-85 -0.43889304115572614 1105 86-87 -0.702719384016568 1105 88-89 -0.9810102564020617 1105 90-91 -0.3425331975067678 1105 92-93 -0.34405925007923344 1105 94-95 -0.2025511293118143 1105 96-97 0.0745726080004907 1105 98-99 -0.5263018088492579 1105 100-101 -0.0425389124258011 1106 1 -0.47605796793548905 1106 2 -0.5498480162771102 1106 3 -0.6850245174675287 1106 4 -0.2463170093891378 1106 5 -0.6819173867825725 1106 6 -0.40488697489456626 1106 7 -0.699970995403902 1106 8 -0.6779387564243748 1106 9 -0.8310007536896507 1106 10-11 -1.172320914240622 1106 12-13 -0.9690009679208273 1106 14-15 -0.7787076218467419 1106 16-17 -0.48059220978911554 1106 18-19 -0.6392344455845844 1106 20-21 -0.4532607336809633 1106 22-23 -0.6068035776152669 1106 24-25 -0.8104945794156961 1106 26-27 -0.847241551473843 1106 28-29 -0.38524568565716777 1106 30-31 -0.6043204615856226 1106 32-33 -0.7438230694889612 1106 34-35 -0.28917072719072223 1106 36-37 -1.0481649618502615 1106 38-39 -0.7006378702259397 1106 40-41 -0.250467797831071 1106 42-43 -0.6583497239319342 1106 44-45 -0.6135808939255085 1106 46-47 -0.5543663534056336 1106 48-49 -0.23512226659428137 1106 50-51 -0.9765502233577763 1106 52-53 -1.3757221641931707 1106 54-55 -1.1307218073580145 1106 56-57 -0.9336768443286232 1106 58-59 -0.7755838918195153 1106 60-61 -1.0211191842315799 1106 62-63 -0.9961629180345284 1106 64-65 -0.9826783327252286 1106 66-67 -1.7614325222164808 1106 68-69 -2.02591229381931 1106 70-71 -1.2704476413919004 1106 72-73 -1.6583619875326185 1106 74-75 -1.4241776056043314 1106 76-77 -1.8068599911173884 1106 78-79 -2.11294856552993 1106 80-81 -2.8145774544442617 1106 82-83 -1.79191047846259 1106 84-85 -1.8126430411557273 1106 86-87 -2.235219384016574 1106 88-89 -2.3503852564020633 1106 90-91 -2.508783197506766 1106 92-93 -1.8390592500792344 1106 94-95 -1.2969261293118173 1106 96-97 -1.4329273919995096 1106 98-99 -1.3769268088492588 1106 100-101 -0.6837889124258005 1107 1 -0.11607474645897753 1107 2 0.037861715266515716 1107 3 -0.03619901411182269 1107 4 -0.2640183516710124 1107 5 0.23590979442548132 1107 6 0.033577790206109626 1107 7 -0.07580992157839717 1107 8 -0.003106541659271045 1107 9 0.1022207228204195 1107 10-11 0.02795173676609153 1107 12-13 0.26157789113957364 1107 14-15 0.12118751238144654 1107 16-17 0.18759151504310267 1107 18-19 -0.10664216370539492 1107 20-21 0.33467970256064916 1107 22-23 0.3723700801028542 1107 24-25 0.5280331051480616 1107 26-27 -0.4624261152322333 1107 28-29 -0.5643983702209283 1107 30-31 -0.9514890857466938 1107 32-33 -0.25311417687150595 1107 34-35 0.08434017884954415 1107 36-37 -0.05999382091066252 1107 38-39 -0.11785682995748914 1107 40-41 0.09921760485348585 1107 42-43 0.1890705780814841 1107 44-45 0.003994609430201024 1107 46-47 0.44309589491651025 1107 48-49 0.32637102199632295 1107 50-51 0.5894103471120218 1107 52-53 0.36423169486723594 1107 54-55 0.5591062127762143 1107 56-57 1.0591083905707066 1107 58-59 1.1441099001268 1107 60-61 1.132781822479835 1107 62-63 1.1150996658580894 1107 64-65 1.228647170630481 1107 66-67 1.4514449945620385 1107 68-69 1.2594484444357228 1107 70-71 1.6127318888094457 1107 72-73 1.129536502400267 1107 74-75 1.2157762534560739 1107 76-77 1.642825411567177 1107 78-79 1.988267877423091 1107 80-81 1.8745165052872856 1107 82-83 1.716436836973653 1107 84-85 1.4559391736093765 1107 86-87 1.7965759179968508 1107 88-89 2.026942763732162 1107 90-91 1.5551010306811541 1107 92-93 1.7257058505919076 1107 94-95 2.214378400889526 1107 96-97 1.9928276415575361 1107 98-99 2.6808122851104734 1107 100-101 2.8281657855607776 1108 1 -0.04738149734725283 1108 2 -0.048745075100640634 1108 3 2.6960017952859516E-4 1108 4 0.214712402375568 1108 5 0.08021496615860002 1108 6 0.3631277309877916 1108 7 -0.11173570128625698 1108 8 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-2.126070542456347 2316 50-51 -2.4867872923232923 2316 52-53 -2.3282006124690326 2316 54-55 -2.2545364625304245 2316 56-57 -2.272469947776898 2316 58-59 -2.9166399263022704 2316 60-61 -2.7648691842315785 2316 62-63 -2.549503435275909 2316 64-65 -2.4141438499666066 2316 66-67 -3.072854936009584 2316 68-69 -2.7407829834744852 2316 70-71 -2.6120424689781068 2316 72-73 -2.2696766427050328 2316 74-75 -2.157690536638814 2316 76-77 -2.4662349911173855 2316 78-79 -2.8601468413920017 2316 80-81 -2.1102671096166716 2316 82-83 -2.4128156508763823 2316 84-85 -2.3982033859833116 2316 86-87 -2.847719384016571 2316 88-89 -2.3039412908848256 2316 90-91 -0.9908952664722825 2316 92-93 -0.9028523535275106 2316 94-95 -0.6310856120704358 2316 96-97 -1.1074963575167516 2316 98-99 -0.877681119194083 2316 100-101 -0.6827113262189037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 986.0 3 198.0 4 133.0 5 168.0 6 188.0 7 214.0 8 231.0 9 324.0 10 417.0 11 585.0 12 795.0 13 1129.0 14 1321.0 15 1814.0 16 2404.0 17 3414.0 18 3048.0 19 1877.0 20 1708.0 21 1690.0 22 1803.0 23 1973.0 24 2242.0 25 2355.0 26 2668.0 27 3167.0 28 3807.0 29 4591.0 30 5539.0 31 6726.0 32 8899.0 33 11776.0 34 16455.0 35 23857.0 36 35780.0 37 52894.0 38 46290.0 39 6246.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06497534779288 19.80085337530767 15.52124565017785 21.612925626721605 2 14.262183149391856 20.496005493170184 41.97028904722043 23.271522310217527 3 18.3231694381121 26.01417159432365 31.329869508700064 24.33278945886419 4 11.541978018586601 17.184697109393987 39.68266215053681 31.59066272148261 5 12.270333866954694 38.2621945339171 35.44544525237719 14.022026346751018 6 29.009371178129545 37.732012854987865 18.867356741345453 14.391259225537137 7 26.321028426803295 34.27073366461928 22.040803728510138 17.36743418006729 8 25.57614708628126 34.45965234432263 20.847767550593698 19.116433018802418 9 25.953129520686662 16.66891696402739 21.78030668338316 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20.44728557760563 26.96695318045739 31.99341841301194 20.592342828925034 36-37 20.41759089593982 26.8388465618671 32.03317581251808 20.710386729674994 38-39 20.285737431719287 27.374587229577507 31.826142641113474 20.513532697589728 40-41 20.358575727181545 27.346076691613302 31.86038885888435 20.434958722320808 42-43 20.039116144860895 27.393259987347047 31.33573533723209 21.23188853055997 44-45 20.356903110315507 27.431308400398148 31.122445042862324 21.089343446424024 46-47 20.423111200274693 27.681048152191945 30.832101975702063 21.063738671831295 48-49 20.47856230685242 27.888484642717977 30.466872176331922 21.16608087409768 50-51 20.447373091257013 28.068649161657707 30.443437936435675 21.04053981064961 52-53 20.634586854903898 28.221115726155727 29.90124490453417 21.243052514406205 54-55 20.30614016474083 27.831748297613768 30.244024768996315 21.618086768649086 56-57 20.574994213409457 28.04123910192115 29.87751716688527 21.50624951778412 58-59 20.795827796304188 27.696396575367565 30.18810790793841 21.31966772038983 60-61 20.646673095467698 27.581870781099326 30.676374156219865 21.095081967213115 62-63 20.46641618318726 27.73500185162326 30.68120448092828 21.1173774842612 64-65 20.26280058322983 27.724246468605106 30.626123450313603 21.386829497851462 66-67 20.217015211508826 27.670789544233337 30.211248454644167 21.900946789613666 68-69 20.047484131775217 27.579948658505778 30.504215132818636 21.868352076900376 70-71 20.37043823425599 27.607928759207432 30.220631207000636 21.80100179953594 72-73 20.189913337332456 27.305973416761987 30.4881694753755 22.015943770530065 74-75 19.932493683337512 27.730244758616703 30.2395509865566 22.09771057148919 76-77 20.061875435182202 27.217669217163877 30.34480896345145 22.375646384202476 78-79 19.910888224515382 27.170990452309766 30.395988041276883 22.522133281897965 80-81 20.105154742571468 27.38632988346571 30.117807257451673 22.390708116511146 82-83 20.077999780119317 27.415649718494862 30.38056717644268 22.12578332494315 84-85 19.85649947441004 27.293452788519957 29.917352183850404 22.932695553219602 86-87 19.81888320956698 27.275725720898837 30.014466197318935 22.89092487221526 88-89 20.024494676747416 26.783675358740933 29.9907421694183 23.20108779509335 90-91 19.768619334828806 26.743407792027433 30.330811086057945 23.157161787085812 92-93 19.97484868707832 26.635703291658793 30.43386014789898 22.95558787336391 94-95 19.576517514268136 26.63565191730172 30.418178346381513 23.369652222048632 96-97 19.45636071847417 26.82955527436578 30.493295166965 23.22078884019505 98-99 19.505378459544378 27.23755296967733 29.50003568280494 23.757032887973352 100-101 19.609102428355698 26.85578950007618 29.737183678271318 23.797924393296803 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 324.0 1 196.0 2 69.5 3 77.5 4 75.5 5 71.5 6 99.5 7 219.5 8 383.0 9 463.5 10 436.0 11 415.5 12 452.0 13 474.0 14 499.5 15 528.0 16 549.0 17 589.0 18 615.5 19 668.5 20 741.5 21 820.0 22 982.5 23 1136.0 24 1329.0 25 1593.5 26 2041.0 27 2616.0 28 2955.5 29 3406.5 30 4059.5 31 4589.5 32 5257.0 33 5931.5 34 6618.0 35 7282.0 36 8058.5 37 8772.5 38 8944.0 39 8920.0 40 8894.0 41 9122.0 42 9228.5 43 9324.5 44 9787.0 45 10023.0 46 9805.5 47 9926.5 48 10843.0 49 10579.5 50 9092.0 51 8050.5 52 7304.0 53 6816.5 54 6327.5 55 5626.5 56 4873.5 57 4178.5 58 3689.0 59 3135.0 60 2486.0 61 2021.5 62 1597.0 63 1124.5 64 781.5 65 571.0 66 399.0 67 284.0 68 197.5 69 140.5 70 94.0 71 61.0 72 56.5 73 45.5 74 30.0 75 25.5 76 22.5 77 14.0 78 8.5 79 7.5 80 5.5 81 3.5 82 2.5 83 1.0 84 1.5 85 2.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19521548786545148 2 0.18558947761567576 3 0.17788866941585518 4 0.1902099625355681 5 0.18404931597571164 6 0.19791077073538868 7 0.20060605360532588 8 0.18905484130559502 9 0.18789972007562195 10-11 0.20195369504029448 12-13 0.20445645770523616 14-15 0.19232768479051876 16-17 0.20079857381032137 18-19 0.19232768479051876 20-21 0.19194264438052774 22-23 0.1888623211005995 24-25 0.18539695741068024 26-27 0.18905484130559502 28-29 0.18828476048561296 30-31 0.18789972007562195 32-33 0.18539695741068024 34-35 0.19444540704546942 36-37 0.18789972007562195 38-39 0.18828476048561296 40-41 0.18982492212557708 42-43 0.18674459884564884 44-45 0.19675564950541558 46-47 0.19791077073538868 48-49 0.20022101319533483 50-51 0.19675564950541558 52-53 0.20657417996018682 54-55 0.1994509323753528 56-57 0.18982492212557708 58-59 0.18231663413075203 60-61 0.17827370982584623 62-63 0.18520443720568472 64-65 0.17981387146581035 66-67 0.18039143208079686 68-69 0.18039143208079686 70-71 0.1846268765906982 72-73 0.18847728069060848 74-75 0.18404931597571164 76-77 0.18578199782067129 78-79 0.18789972007562195 80-81 0.18327923515572958 82-83 0.18539695741068024 84-85 0.18501191700068922 86-87 0.18789972007562195 88-89 0.1821241139257565 90-91 0.1873221594606354 92-93 0.18558947761567576 94-95 0.18539695741068024 96-97 0.19136508376554118 98-99 0.18655207864065332 100-101 0.17846623003084175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 259713.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.51808847151217 #Duplication Level Percentage of deduplicated Percentage of total 1 88.37958247544029 64.09118381029286 2 6.984796906218227 10.130482400013557 3 1.8235590961405792 3.9672305960085996 4 0.7811736866335669 2.2659689007564134 5 0.4757838191048341 1.725146654357915 6 0.31385033562105713 1.3655895843228967 7 0.1960433117550369 0.9951680358270006 8 0.16160795266017694 0.937559984696853 9 0.12240889681314408 0.798917328791621 >10 0.7155241430152108 8.976786190985223 >50 0.03292443429148483 1.579678240848005 >100 0.01115182451808357 1.669791529087506 >500 5.310392627658843E-4 0.3230984992860742 >1k 0.0010620785255317686 1.1733982447254685 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1695 0.6526434949347933 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1352 0.5205746343078705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.32304890398247293 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 476 0.18327923515572958 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 459 0.1767335481858821 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 375 0.14439015374663572 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 364 0.1401547092367344 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 324 0.12475309283709327 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 302 0.11628220381729062 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9252020499551428E-4 0.0 12-13 0.0 0.0 0.0 3.8504040999102855E-4 0.0 14-15 0.0 0.0 0.0 3.8504040999102855E-4 0.0 16-17 1.9252020499551428E-4 0.0 0.0 3.8504040999102855E-4 0.0 18-19 3.8504040999102855E-4 0.0 0.0 3.8504040999102855E-4 0.0 20-21 3.8504040999102855E-4 0.0 0.0 3.8504040999102855E-4 0.0 22-23 3.8504040999102855E-4 0.0 0.0 3.8504040999102855E-4 0.0 24-25 3.8504040999102855E-4 0.0 0.0 3.8504040999102855E-4 0.0 26-27 3.8504040999102855E-4 0.0 0.0 5.775606149865428E-4 0.0 28-29 3.8504040999102855E-4 0.0 0.0 0.0030803232799282284 0.0 30-31 3.8504040999102855E-4 0.0 0.0 0.009240969839784684 0.0 32-33 3.8504040999102855E-4 0.0 0.0 0.015401616399641143 0.0 34-35 3.8504040999102855E-4 0.0 0.0 0.02194730336948863 0.0 36-37 3.8504040999102855E-4 0.0 0.0 0.02926307115931817 0.0 38-39 3.8504040999102855E-4 0.0 0.0 0.03850404099910286 0.0 40-41 3.8504040999102855E-4 0.0 0.0 0.047937531043883055 0.0 42-43 3.8504040999102855E-4 0.0 0.0 0.07046239502835823 0.0 44-45 3.8504040999102855E-4 0.0 0.0 0.09991798639267191 0.0 46-47 3.8504040999102855E-4 0.0 0.0 0.13726690616180168 0.0 48-49 3.8504040999102855E-4 0.0 0.0 0.21774035184992663 0.0 50-51 3.8504040999102855E-4 0.0 0.0 0.3016791612279709 0.0 52-53 3.8504040999102855E-4 0.0 0.0 0.4058325921305441 0.0 54-55 3.8504040999102855E-4 0.0 0.0 0.593347271796175 0.0 56-57 3.8504040999102855E-4 0.0 0.0 0.7979962497064067 0.0 58-59 3.8504040999102855E-4 0.0 0.0 1.0715674610050325 0.0 60-61 3.8504040999102855E-4 0.0 0.0 1.3593851674733264 0.0 62-63 3.8504040999102855E-4 0.0 0.0 1.7617523959139512 0.0 64-65 3.8504040999102855E-4 0.0 0.0 2.068629602676801 0.0 66-67 3.8504040999102855E-4 0.0 0.0 2.458290497587722 0.0 68-69 3.8504040999102855E-4 0.0 0.0 2.9413236919214674 0.0 70-71 3.8504040999102855E-4 0.0 0.0 3.361210259016684 0.0 72-73 3.8504040999102855E-4 0.0 0.0 3.979970197872267 0.0 74-75 3.8504040999102855E-4 0.0 0.0 4.687096910820791 0.0 76-77 3.8504040999102855E-4 0.0 0.0 5.278326460362015 0.0 78-79 3.8504040999102855E-4 0.0 0.0 5.854731954118584 0.0 80-81 7.700808199820571E-4 0.0 0.0 6.588618975561485 0.0 82-83 7.700808199820571E-4 0.0 0.0 7.278803910470404 0.0 84-85 7.700808199820571E-4 0.0 0.0 8.068714311567 0.0 86-87 7.700808199820571E-4 0.0 0.0 9.29487549718343 0.0 88-89 7.700808199820571E-4 0.0 0.0 10.673127644746316 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1220 0.0 36.602818 1 GTATAGG 85 1.625994E-5 33.533497 1 CCAGTAC 95 3.4822224E-5 29.997868 3 GTAAGGT 80 4.48095E-4 29.691118 4 GTATCAA 1855 0.0 28.68274 1 CATGGGA 860 0.0 28.172035 4 GTACATG 1390 0.0 27.34146 1 TACATGG 1430 0.0 26.571539 2 GAGTATT 90 8.9224376E-4 26.392105 1 GTATCAC 90 8.9224376E-4 26.392105 1 ACATGGG 1370 0.0 26.348494 3 ATGGGAG 315 0.0 25.638044 5 GGTCTGC 95 0.0012245007 24.998222 8 CAACGCA 2200 0.0 24.184763 5 ATCAACG 2180 0.0 24.184061 3 TCAACGC 2205 0.0 24.129925 4 AACGCAG 2235 0.0 24.018585 6 TATCAAC 2215 0.0 23.587488 2 GAGTACT 1235 0.0 23.459871 12-13 GTATAAA 170 3.1301006E-6 22.355663 1 >>END_MODULE