FastQCFastQC Report
Thu 26 May 2016
SRR937320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259713
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.7542941631724249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18310.7050089906935733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.4543476837894137No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10140.39043097573090296No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.16017681055626787No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2730.1051160319275508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG704.2874217E-640.7279051
GTCATAG753.0659142E-431.677261
CTGGTCG600.003942630731.6711629
GTGTAGG953.4726138E-530.0100361
GGTAAGG953.4817596E-529.9984823
TAAGGTG953.4817596E-529.9984825
ACTATAC650.005837299429.229293
TAGGACA1004.9402628E-528.4985584
TAGTCGG350.00838160527.1467140-41
GTAAGGT1109.452217E-525.907784
GTACATG15800.025.2616121
GTATCAA28050.025.240171
GTATATG1701.11223926E-725.1554721
GTATACA950.001221613425.0083641
TGTAGGG950.001224360424.9987352
GCTACAC950.001224360424.9987353
AGTACTT17100.024.7257312-13
TACATGG16250.024.2603112
ACATGGG16050.023.9707493
GCCTATG1000.001647001523.7579461
Produced by FastQC (version 0.11.5)