##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937320_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 259713 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42580463819678 33.0 31.0 34.0 31.0 34.0 2 32.62556745330422 34.0 31.0 34.0 31.0 34.0 3 32.670613330869074 34.0 31.0 34.0 31.0 34.0 4 36.258215799748186 37.0 35.0 37.0 35.0 37.0 5 36.210397631231395 37.0 35.0 37.0 35.0 37.0 6 36.27568123274538 37.0 37.0 37.0 35.0 37.0 7 36.230970340337215 37.0 37.0 37.0 35.0 37.0 8 36.208780461509434 37.0 36.0 37.0 35.0 37.0 9 37.95453057798416 39.0 38.0 39.0 35.0 39.0 10-11 37.878123159025535 39.0 38.0 39.0 35.0 39.0 12-13 37.79731472818072 39.0 37.5 39.0 35.0 39.0 14-15 39.00199643452581 40.0 38.0 41.0 36.0 41.0 16-17 38.97894213997759 40.0 38.0 41.0 36.0 41.0 18-19 39.01405975057082 40.0 38.0 41.0 36.0 41.0 20-21 38.95056851216535 40.0 38.0 41.0 35.0 41.0 22-23 38.80250699810945 40.0 38.0 41.0 35.0 41.0 24-25 38.730902958265474 40.0 38.0 41.0 35.0 41.0 26-27 38.6782294301787 40.0 38.0 41.0 35.0 41.0 28-29 38.555542079141205 40.0 38.0 41.0 34.0 41.0 30-31 38.603662504379834 40.0 38.0 41.0 35.0 41.0 32-33 38.50067189551544 40.0 38.0 41.0 35.0 41.0 34-35 38.351308944873765 40.0 38.0 41.0 34.5 41.0 36-37 38.1674579247092 40.0 38.0 41.0 34.0 41.0 38-39 38.02655238667298 40.0 37.5 41.0 34.0 41.0 40-41 37.84025443470292 40.0 37.0 41.0 33.0 41.0 42-43 37.60713941928205 40.0 37.0 41.0 33.0 41.0 44-45 37.23022528714388 40.0 37.0 41.0 33.0 41.0 46-47 36.73743131841687 40.0 37.0 41.0 31.5 41.0 48-49 36.29835241208565 40.0 36.0 41.0 30.0 41.0 50-51 36.004312452591904 40.0 36.0 41.0 29.5 41.0 52-53 35.772708720780244 39.0 35.5 41.0 28.5 41.0 54-55 35.56357017168952 39.0 35.0 41.0 28.0 41.0 56-57 35.29051106413618 39.0 35.0 40.0 27.0 41.0 58-59 35.019893112782185 38.5 35.0 40.0 26.5 41.0 60-61 34.67486417699538 38.0 34.5 40.0 26.0 41.0 62-63 34.320725185108174 38.0 34.0 40.0 25.5 41.0 64-65 33.95521402471189 37.5 34.0 40.0 23.5 41.0 66-67 33.5472021038608 37.0 33.5 39.5 22.5 41.0 68-69 33.11746427787596 36.0 33.0 39.0 21.5 40.5 70-71 32.6973601629491 36.0 33.0 39.0 19.5 40.0 72-73 32.207496351742115 35.0 32.0 38.5 18.0 40.0 74-75 31.720886131999553 35.0 32.0 37.5 14.5 39.5 76-77 30.474824132792737 34.0 30.5 36.0 12.0 39.0 78-79 31.04981845344669 35.0 31.0 36.0 9.5 39.0 80-81 30.81836103699083 35.0 31.0 36.0 7.0 38.5 82-83 30.478782348207446 34.5 31.0 36.0 4.0 37.0 84-85 30.10384154817048 34.0 31.0 35.0 2.0 37.0 86-87 29.7252274626222 34.0 30.5 35.0 2.0 36.0 88-89 29.295566644719365 34.0 30.0 35.0 2.0 36.0 90-91 28.821870680327898 34.0 29.0 35.0 2.0 35.5 92-93 28.478784273409495 34.0 29.0 35.0 2.0 35.0 94-95 28.065221995048383 34.0 29.0 35.0 2.0 35.0 96-97 27.60782671641389 34.0 28.0 35.0 2.0 35.0 98-99 26.900632621393612 33.0 26.5 35.0 2.0 35.0 100-101 24.511479979823882 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.19858524997855653 1101 2 0.007280296106600304 1101 3 -0.04443658463075195 1101 4 -0.11922908963347112 1101 5 -0.08731027773543332 1101 6 0.04648288976019188 1101 7 -0.18643351765562244 1101 8 -0.1153038041211829 1101 9 -0.2074389668774188 1101 10-11 -0.09992094107481364 1101 12-13 -0.3472980905200558 1101 14-15 -0.30417398298147447 1101 16-17 -0.28120055081775064 1101 18-19 -0.12959582600571906 1101 20-21 -0.17569235859669874 1101 22-23 0.008453786523709539 1101 24-25 0.048155544512376025 1101 26-27 0.024986469176894843 1101 28-29 -0.28738608981657165 1101 30-31 -0.3876429172611111 1101 32-33 -0.23447477050282828 1101 34-35 -0.16875967013510262 1101 36-37 -0.012264200515481605 1101 38-39 0.05791376643499291 1101 40-41 -0.14615309661704856 1101 42-43 -0.3533615623374686 1101 44-45 -0.6095569700049239 1101 46-47 -0.6774902469886825 1101 48-49 -0.47962703001242346 1101 50-51 -0.6425401620561715 1101 52-53 -0.4579315797718593 1101 54-55 -0.41722451006258865 1101 56-57 -0.3295063878334261 1101 58-59 -0.444912160294507 1101 60-61 -0.46142624026705903 1101 62-63 -0.4121366585365678 1101 64-65 -0.25408540457999607 1101 66-67 -0.20761234402534257 1101 68-69 -0.25701345254404373 1101 70-71 -0.22621456240647575 1101 72-73 -0.2994876184379258 1101 74-75 -0.4392901333294148 1101 76-77 -0.39590694771166923 1101 78-79 -0.3683016303778537 1101 80-81 -0.18661984566406886 1101 82-83 -0.2511470641366458 1101 84-85 -0.6469898700030043 1101 86-87 -0.5253116527684583 1101 88-89 -0.3828778137347406 1101 90-91 -0.3221444732640606 1101 92-93 -0.7997851005907712 1101 94-95 -0.3474394489475934 1101 96-97 -0.35872527910136753 1101 98-99 -0.26507249288094314 1101 100-101 0.07509387910057086 1102 1 -0.22043130923383103 1102 2 -0.24329611706636456 1102 3 -0.3001574853724023 1102 4 -0.12596832031402272 1102 5 -0.3797486975947848 1102 6 -0.13690776466089716 1102 7 -0.30574143757262107 1102 8 -0.289495864990414 1102 9 -0.5853546568439825 1102 10-11 -0.3577121257960272 1102 12-13 -0.20857465356044003 1102 14-15 -0.5626160342438169 1102 16-17 -0.37995934111863505 1102 18-19 -0.35601572378844537 1102 20-21 -0.2328461264174706 1102 22-23 -0.24777387746583912 1102 24-25 -0.43904808304785803 1102 26-27 -0.41789338710408686 1102 28-29 -0.3571344854665668 1102 30-31 -0.4529024953266685 1102 32-33 -0.27191040436518676 1102 34-35 -0.390222414630351 1102 36-37 -0.28996085347632317 1102 38-39 -0.3280116649141931 1102 40-41 -0.22326997399475346 1102 42-43 0.04235328606586819 1102 44-45 0.009871035989775123 1102 46-47 0.17316350161832617 1102 48-49 0.2615946777076701 1102 50-51 0.39220554366568905 1102 52-53 0.4295117395994623 1102 54-55 0.20599101082277826 1102 56-57 0.38732823922725146 1102 58-59 0.6420013393020056 1102 60-61 0.27735772007571313 1102 62-63 0.547240623098908 1102 64-65 0.5178206977141286 1102 66-67 0.386722475173066 1102 68-69 0.41151054545772325 1102 70-71 0.28156701542852147 1102 72-73 0.4932366062863629 1102 74-75 0.46646631289123874 1102 76-77 0.19539689888557277 1102 78-79 0.18318407457545405 1102 80-81 0.41091166492271824 1102 82-83 0.6218042673777866 1102 84-85 0.3539876306694758 1102 86-87 0.24134188448704563 1102 88-89 0.42186462564581007 1102 90-91 0.08198600746452556 1102 92-93 -0.07722476934533873 1102 94-95 -0.14524571648016504 1102 96-97 -0.09113507043980107 1102 98-99 -0.11915589986707076 1102 100-101 0.518667832910662 1103 1 0.04522359916103369 1103 2 0.003627590890978638 1103 3 0.05563277987233306 1103 4 -0.009308489851896695 1103 5 0.05363248917031882 1103 6 -0.010130351480697186 1103 7 -0.04664928553543035 1103 8 -0.019875957962796065 1103 9 0.0734503746025581 1103 10-11 -0.014329571790007378 1103 12-13 -0.15480790485931806 1103 14-15 0.10994685883794375 1103 16-17 0.07123015847118808 1103 18-19 0.09818170579864471 1103 20-21 0.03255242430716976 1103 22-23 0.17075565493777134 1103 24-25 0.03504270174842361 1103 26-27 0.3061119451354486 1103 28-29 0.11912679427091888 1103 30-31 0.16348388695389104 1103 32-33 0.32019036128971834 1103 34-35 0.4237644598569261 1103 36-37 0.28595519793193347 1103 38-39 0.17685913082001292 1103 40-41 0.2689196623191563 1103 42-43 0.4275905023633797 1103 44-45 0.7699886187078491 1103 46-47 1.3518789787854004 1103 48-49 1.24667466130078 1103 50-51 0.8564009223914226 1103 52-53 1.012451991171794 1103 54-55 1.0744111639537621 1103 56-57 0.9392663032141328 1103 58-59 0.6701665020036742 1103 60-61 0.6436757403108828 1103 62-63 0.9223773471896948 1103 64-65 0.6702851494505211 1103 66-67 0.764730405170333 1103 68-69 0.8254085492860028 1103 70-71 0.8756331898884611 1103 72-73 0.873654298383709 1103 74-75 0.9653041581195652 1103 76-77 1.244275761341136 1103 78-79 1.0378976376052584 1103 80-81 0.9216957775833698 1103 82-83 1.0218760475254456 1103 84-85 1.1515505237512365 1103 86-87 1.1359276104919687 1103 88-89 1.0086331517601081 1103 90-91 1.7168472325123787 1103 92-93 1.845850839076867 1103 94-95 1.2144240948405916 1103 96-97 1.5833590504242956 1103 98-99 1.8586435258917469 1103 100-101 2.3302962168319112 1104 1 -0.018544516780998777 1104 2 -5.530111157128204E-4 1104 3 -0.0914129280429421 1104 4 -0.1979929937537932 1104 5 -0.09480675274718209 1104 6 -0.08933882416742733 1104 7 -0.3090796088353187 1104 8 -0.24406770824150215 1104 9 -0.16757526642308562 1104 10-11 -0.25836524626046753 1104 12-13 -0.1749026651714729 1104 14-15 -0.18941211552103 1104 16-17 -0.28035847029135397 1104 18-19 0.16328761438281703 1104 20-21 -0.12996151103285314 1104 22-23 -0.3248965189752724 1104 24-25 -0.26448349669082205 1104 26-27 -0.3109449110518412 1104 28-29 -0.14742838967556793 1104 30-31 -0.09822179866484504 1104 32-33 -0.373343640939936 1104 34-35 -0.42774055686547996 1104 36-37 -0.30788538177821323 1104 38-39 -0.35081645446427245 1104 40-41 -0.18838245585252622 1104 42-43 -0.5487751609588116 1104 44-45 -0.5147661193077582 1104 46-47 -0.533349560790974 1104 48-49 -0.2572551045855036 1104 50-51 -0.11202159711805137 1104 52-53 -0.26383006011025856 1104 54-55 -0.061987944184473065 1104 56-57 -0.24175376367550427 1104 58-59 -0.24711331962397765 1104 60-61 -0.23051600996782895 1104 62-63 -0.08534283118265051 1104 64-65 0.015407780442714625 1104 66-67 0.16604032713242844 1104 68-69 -0.03348219764822602 1104 70-71 0.2006889312485498 1104 72-73 -0.071440461928443 1104 74-75 0.1126564435153341 1104 76-77 0.10065814707134635 1104 78-79 -0.03272666562773807 1104 80-81 0.24521863154100743 1104 82-83 0.2607556461876541 1104 84-85 0.2541146263153422 1104 86-87 0.3266466740371179 1104 88-89 0.2854168752829622 1104 90-91 0.48142359817570224 1104 92-93 0.31129119582157116 1104 94-95 0.38557794099443754 1104 96-97 0.2213467873250785 1104 98-99 0.32110171987167746 1104 100-101 0.8249450462633483 1105 1 0.01645548321900492 1105 2 -0.020937626500327156 1105 3 -0.18987446650447737 1105 4 -0.05568530144609696 1105 5 -0.07172982967026087 1105 6 -0.005492670321274318 1105 7 -0.07215653191224192 1105 8 -0.20176001593381443 1105 9 -0.4648829587307759 1105 10-11 -0.04624986164508016 1105 12-13 -0.1745180497868546 1105 14-15 -0.1592198078287268 1105 16-17 -0.09420462413750386 1105 18-19 -0.2347893086941042 1105 20-21 -0.10284612641746804 1105 22-23 -0.29489651897527125 1105 24-25 -0.23640657361389827 1105 26-27 0.1532858581789327 1105 28-29 -0.014351466598647278 1105 30-31 0.13620127825823403 1105 32-33 -0.22238210247839163 1105 34-35 -0.5017790184039441 1105 36-37 -0.43538538177821096 1105 38-39 -0.45216260831042376 1105 40-41 -0.35742091739098214 1105 42-43 -0.4364674686511165 1105 44-45 -0.09399688853852695 1105 46-47 -0.8756572530986588 1105 48-49 -0.5982166430470457 1105 50-51 -0.9029831355795963 1105 52-53 -0.9167146754948803 1105 54-55 -1.1366033287998576 1105 56-57 -1.0754076098293481 1105 58-59 -1.101536396547047 1105 60-61 -1.6532083176601304 1105 62-63 -1.5434197542595811 1105 64-65 -1.3042076041726673 1105 66-67 -1.31030582671373 1105 68-69 -0.7288668130328446 1105 70-71 -0.7277726072129909 1105 72-73 -0.3660558465438264 1105 74-75 -0.6354204795615921 1105 76-77 -0.45068800677480425 1105 78-79 -0.5619574348585061 1105 80-81 -0.8305505992282249 1105 82-83 -1.108667430735423 1105 84-85 -1.0251161429154294 1105 86-87 -0.6295071721167247 1105 88-89 -0.2978523554862669 1105 90-91 -0.41626870951660777 1105 92-93 -0.5475549580245804 1105 94-95 -0.478075905159411 1105 96-97 -0.29019167421338565 1105 98-99 -0.16255212628216853 1105 100-101 0.15975273857104 1106 1 -0.25604451678099593 1106 2 -0.16593762650032318 1106 3 -0.07362446650447652 1106 4 -0.18068530144609696 1106 5 -0.40922982967025945 1106 6 -0.2604926703212769 1106 7 -0.1696565319122456 1106 8 -0.258010015933813 1106 9 -0.359882958730779 1106 10-11 -0.3893748616450807 1106 12-13 -0.3095180497868597 1106 14-15 -0.8879698078287177 1106 16-17 -0.2654546241375044 1106 18-19 -0.30666430869410277 1106 20-21 -0.254721126417472 1106 22-23 -0.49052151897527807 1106 24-25 -0.307031573613898 1106 26-27 -0.501089141821069 1106 28-29 -0.4462264665986453 1106 30-31 -0.165048721741762 1106 32-33 -0.09425710247839447 1106 34-35 -0.5705290184039384 1106 36-37 -0.29851038177820755 1106 38-39 -0.86716260831043 1106 40-41 -0.4249209173909847 1106 42-43 -0.3814674686511097 1106 44-45 -1.040246888538526 1106 46-47 -0.8725322530986617 1106 48-49 -0.8207166430470494 1106 50-51 -0.7004831355795957 1106 52-53 -1.0229646754948831 1106 54-55 -1.0684783287998627 1106 56-57 -0.7347826098293524 1106 58-59 -0.7615363965470507 1106 60-61 -0.5257083176601327 1106 62-63 -0.44279475425957315 1106 64-65 -0.6285826041726637 1106 66-67 -0.6034308267137263 1106 68-69 -0.7051168130328378 1106 70-71 -0.8971476072129931 1106 72-73 -0.8466808465438298 1106 74-75 -0.8322954795615907 1106 76-77 -0.6750630067748062 1106 78-79 -0.5863324348585088 1106 80-81 -1.257425599228224 1106 82-83 -1.1755424307354225 1106 84-85 -0.9369911429154278 1106 86-87 -1.1913821721167253 1106 88-89 -0.4203523554862656 1106 90-91 -0.8043937095166029 1106 92-93 -1.1550549580245821 1106 94-95 -0.8012009051594084 1106 96-97 -0.12456667421338352 1106 98-99 0.2874478737178343 1106 100-101 0.3547527385710403 1107 1 -0.08968545637831227 1107 2 -0.13942755938623463 1107 3 -0.02215634569910918 1107 4 -0.10132288533871758 1107 5 -0.30226674242193496 1107 6 -0.20495575756959994 1107 7 -0.027122974865264382 1107 8 -0.13095464680629476 1107 9 -0.2783393345697007 1107 10-11 -0.05797804956453945 1107 12-13 -0.14139724441773183 1107 14-15 0.023817104922962073 1107 16-17 -0.07933885232542082 1107 18-19 -0.06801078520417292 1107 20-21 -0.3976951197060572 1107 22-23 -0.26566833105581367 1107 24-25 -0.42378912394946866 1107 26-27 -0.41587521564657237 1107 28-29 -0.5367675739811943 1107 30-31 -0.5322047620102168 1107 32-33 -0.25300290784751667 1107 34-35 -0.2904535150482346 1107 36-37 -0.020301489160758024 1107 38-39 -0.11355522576008781 1107 40-41 -0.4240819912164895 1107 42-43 -0.0848231733490934 1107 44-45 -0.1339968885385261 1107 46-47 0.2565575120020114 1107 48-49 0.40763906165094 1107 50-51 0.9844161932794648 1107 52-53 0.9674296198071417 1107 54-55 0.9364503624753056 1107 56-57 0.8827299740632668 1107 58-59 0.701802529627443 1107 60-61 1.0544091320042952 1107 62-63 0.9326876282907577 1107 64-65 0.6574702481763239 1107 66-67 0.8506169920782227 1107 68-69 1.09677077085977 1107 70-71 1.1570260505051237 1107 72-73 1.064917307818586 1107 74-75 1.6209553593645865 1107 76-77 1.4855703824869373 1107 78-79 1.5508949141347763 1107 80-81 1.036093696073788 1107 82-83 0.8319869316806852 1107 84-85 1.083877145675178 1107 86-87 1.6204257137893165 1107 88-89 1.2145469733727907 1107 90-91 1.213831961624333 1107 92-93 1.4225457131163601 1107 94-95 1.5323771149748175 1107 96-97 2.1537680573302396 1107 98-99 2.2994445180131358 1107 100-101 2.2830245506515787 1108 1 0.07410254204253164 1108 2 -0.18211409708855797 1108 3 -0.27546270179859533 1108 4 -0.32921471321080276 1108 5 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-2.23611122721902 2316 54-55 -3.0905688460412435 2316 56-57 -2.5491145063810805 2316 58-59 -2.7343812241332586 2316 60-61 -2.295104869384275 2316 62-63 -2.362730099087166 2316 64-65 -2.8011041558968053 2316 66-67 -2.8179782405068323 2316 68-69 -2.8178323302742214 2316 70-71 -3.5024277796267853 2316 72-73 -2.7017455017162426 2316 74-75 -2.654385996802972 2316 76-77 -2.7161190412575635 2316 78-79 -2.9976470900309202 2316 80-81 -3.3484816337109855 2316 82-83 -2.31358122383887 2316 84-85 -3.3513230394671503 2316 86-87 -3.248817516944314 2316 88-89 -2.727248907210406 2316 90-91 -2.6460962957235026 2316 92-93 -2.075313578714237 2316 94-95 -2.264024181021476 2316 96-97 -2.0263985707651067 2316 98-99 -1.5129831607649251 2316 100-101 -2.6344713993599953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 2.0 6 7.0 7 27.0 8 44.0 9 101.0 10 168.0 11 321.0 12 564.0 13 832.0 14 1059.0 15 1571.0 16 2505.0 17 3799.0 18 3409.0 19 1866.0 20 1392.0 21 1329.0 22 1401.0 23 1417.0 24 1563.0 25 1779.0 26 2023.0 27 2462.0 28 2884.0 29 3732.0 30 4708.0 31 5882.0 32 7765.0 33 10523.0 34 14960.0 35 22856.0 36 36454.0 37 59259.0 38 54832.0 39 6213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.380794982625645 20.686652952099177 15.859202243641604 20.073349821633574 2 15.499801704188855 21.885311863480073 40.56323711173488 22.051649320596198 3 19.377928713618495 26.599746643410228 31.312641261700414 22.709683381270864 4 12.396761040071155 18.416097769460904 38.86905930777435 30.318081882693587 5 12.450762578404458 39.43691256820081 34.33816819464713 13.774156658747607 6 29.52104823401216 38.614932637180274 17.596346736590004 14.267672392217563 7 26.90007816320323 35.148798866441034 20.977771617131218 16.973351353224523 8 26.307500972227032 35.31128591945725 19.515003099575303 18.866210008740417 9 26.734125746497096 17.27599311547747 20.886131999553353 35.10374913847208 10-11 24.412956634740336 28.035423785917118 27.33907686383982 20.212542715502718 12-13 26.07499451287837 25.77329908856022 28.01974593664204 20.131960461919377 14-15 22.695051845691204 26.837894136989675 27.638778189771017 22.8282758275481 16-17 20.546911398351256 30.225287143885748 28.130089752919567 21.097711704843423 18-19 20.44333552806367 28.116805858774878 31.8284028908834 19.611455722278055 20-21 21.535117610593232 26.60783249202004 32.27350960483303 19.583540292553703 22-23 21.193720664281944 26.81558975622716 31.93632103623671 20.05436854325418 24-25 21.250654571217346 26.950429706752093 32.16524457861015 19.633671143420404 26-27 20.836542015771318 27.257500616067027 32.106333169048796 19.799624199112863 28-29 20.272463511413935 27.733443201792806 32.27148547676301 19.722607810030247 30-31 20.70659037703302 27.300817828979795 31.94172775998029 20.0508640340069 32-33 20.616218672149643 27.66823378113533 31.4377793949475 20.27776815176753 34-35 20.907501742307854 27.526346390053636 31.83514109805824 19.73101076958027 36-37 21.193779287136184 27.06699318093434 31.75486017257511 19.984367359354362 38-39 20.78802673730902 27.64331259241005 31.906496426811238 19.66216424346969 40-41 20.74501468929164 27.88462649155029 31.582169548694132 19.788189270463935 42-43 20.764910537736267 28.063213710166522 30.97899230044996 20.19288345164725 44-45 21.14430159445234 27.630114780546215 30.79225914759754 20.433324477403904 46-47 21.35491869871743 28.39673023683836 30.13480264753786 20.113548416906355 48-49 21.329120991248033 28.65875023583725 29.644646205619278 20.367482567295436 50-51 21.045153688879648 28.85531336513767 29.636175316599477 20.463357629383204 52-53 21.273482652004326 29.19953949166965 29.05649697935798 20.47048087696804 54-55 20.95794203601668 28.983916862074675 29.169891380100342 20.888249721808304 56-57 21.379176244546866 28.76271114653483 29.383781327850357 20.474331281067947 58-59 21.654480137690452 28.288918922040867 29.230342724468933 20.826258215799747 60-61 21.190698963856256 28.739993762345357 29.82041715278018 20.2488901210182 62-63 21.058630103229333 28.445052808292232 30.122866394828137 20.373450693650298 64-65 20.80161562956032 28.56691809805439 29.862964118084196 20.768502154301093 66-67 20.816632205549972 28.695329074786397 29.21686631011925 21.27117240954438 68-69 20.817402286369955 28.35803367563426 29.546268380866568 21.278295657129213 70-71 21.193394246726193 28.658557715632256 29.286366104122628 20.861681933518923 72-73 21.014928016695354 28.145876409729205 29.577456654075846 21.2617389194996 74-75 20.858024049624007 28.656247473172307 28.948493144355503 21.53723533284818 76-77 20.78274864947076 28.173984359658544 29.394754979535104 21.648512011335587 78-79 20.765806871431156 28.05673955481628 29.08710769195227 22.090345881800292 80-81 20.801230589150332 28.515130162910594 29.00682676646914 21.67681248146993 82-83 20.832033821949615 28.678387296746795 28.81064867757871 21.67893020372488 84-85 20.598506813290054 28.563260214159474 28.67761721592681 22.16061575662366 86-87 20.408874411369474 28.562490133339498 28.36688960506405 22.661745850226982 88-89 20.346112824540935 28.200937188357916 28.609272543153402 22.843677443947744 90-91 20.411400934932114 28.0745327264746 28.65039391909064 22.863672419502652 92-93 20.62042636263349 27.966354586007004 28.51235134776105 22.900867703598458 94-95 20.52496409498177 28.31452410930527 28.3089410233604 22.85157077235256 96-97 20.507444756327175 28.561335012109524 28.189963536673172 22.741256694890126 98-99 20.26679450008278 28.973135730594922 27.376758190772122 23.38331157855017 100-101 20.113402260343864 29.19636496659543 26.863243420165965 23.826989352894742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 127.0 1 88.0 2 56.5 3 67.0 4 69.0 5 74.0 6 102.5 7 223.0 8 401.0 9 510.0 10 510.5 11 480.0 12 510.5 13 573.0 14 623.0 15 632.5 16 650.0 17 671.0 18 691.0 19 766.0 20 808.0 21 882.5 22 1019.5 23 1166.0 24 1320.5 25 1562.0 26 1976.5 27 2425.5 28 2819.5 29 3330.5 30 4015.0 31 4619.5 32 5197.0 33 5830.0 34 6493.0 35 7042.0 36 7806.5 37 8432.0 38 8701.5 39 8827.0 40 8714.5 41 8912.5 42 9077.5 43 9193.5 44 9681.5 45 9767.0 46 9479.5 47 10021.0 48 11142.0 49 10552.0 50 9008.0 51 8131.5 52 7376.5 53 6847.0 54 6391.5 55 5874.5 56 5135.5 57 4412.0 58 3943.0 59 3395.5 60 2758.0 61 2179.5 62 1643.5 63 1170.5 64 816.5 65 578.5 66 401.0 67 298.0 68 214.0 69 135.5 70 97.0 71 75.0 72 54.0 73 43.0 74 35.5 75 28.0 76 22.5 77 18.5 78 13.0 79 11.0 80 9.0 81 4.5 82 4.0 83 4.0 84 3.5 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051980455348788854 2 0.0 3 0.0 4 0.0 5 7.700808199820571E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.9252020499551428E-4 12-13 0.0053905657398743995 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015401616399641142 24-25 3.8504040999102855E-4 26-27 3.8504040999102855E-4 28-29 0.0032728434849237423 30-31 3.8504040999102855E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.8504040999102855E-4 40-41 0.0 42-43 0.008663409224798142 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.005775606149865429 92-93 0.0017326818449596288 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.006930727379838515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 259713.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.20237849280132 #Duplication Level Percentage of deduplicated Percentage of total 1 86.99392407873816 60.20186460670861 2 7.725536467133019 10.692509973169567 3 2.0918313960331147 4.342791240342256 4 0.9540689945872864 2.6409537468670323 5 0.5375334699733897 1.8599297320823687 6 0.336844741559873 1.3986274379241708 7 0.24619965146522732 1.19263210258447 8 0.17298030999014333 0.9576519106992001 9 0.12561468518633662 0.7823551489667055 >10 0.7477065246495853 9.33412302127367 >50 0.046479780964955524 2.125997772382176 >100 0.019039910274801057 2.151574850941641 >500 0.0 0.0 >1k 0.0022399894440942422 2.318988456058137 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1959 0.7542941631724249 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1831 0.7050089906935733 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.4543476837894137 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1014 0.39043097573090296 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.16017681055626787 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 273 0.1051160319275508 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9252020499551428E-4 0.0 12-13 0.0 0.0 0.0 3.8504040999102855E-4 0.0 14-15 0.0 0.0 0.0 3.8504040999102855E-4 0.0 16-17 0.0 0.0 0.0 3.8504040999102855E-4 0.0 18-19 0.0 0.0 0.0 3.8504040999102855E-4 0.0 20-21 0.0 0.0 0.0 3.8504040999102855E-4 0.0 22-23 0.0 0.0 0.0 3.8504040999102855E-4 3.8504040999102855E-4 24-25 0.0 0.0 0.0 3.8504040999102855E-4 3.8504040999102855E-4 26-27 0.0 0.0 0.0 5.775606149865428E-4 3.8504040999102855E-4 28-29 0.0 0.0 0.0 0.0030803232799282284 3.8504040999102855E-4 30-31 0.0 0.0 0.0 0.009240969839784684 7.700808199820571E-4 32-33 0.0 0.0 0.0 0.015401616399641143 7.700808199820571E-4 34-35 0.0 0.0 0.0 0.021562262959497598 7.700808199820571E-4 36-37 0.0 0.0 0.0 0.02887803074932714 7.700808199820571E-4 38-39 3.8504040999102855E-4 0.0 0.0 0.03754143997412528 7.700808199820571E-4 40-41 5.775606149865428E-4 0.0 0.0 0.04639736940391894 7.700808199820571E-4 42-43 7.700808199820571E-4 0.0 0.0 0.0687297131833986 7.700808199820571E-4 44-45 7.700808199820571E-4 0.0 0.0 0.09799278434271677 7.700808199820571E-4 46-47 7.700808199820571E-4 0.0 0.0 0.13726690616180168 7.700808199820571E-4 48-49 7.700808199820571E-4 0.0 0.0 0.2194730336948863 7.700808199820571E-4 50-51 7.700808199820571E-4 0.0 0.0 0.30398940368791705 7.700808199820571E-4 52-53 7.700808199820571E-4 0.0 0.0 0.4089129154104723 7.700808199820571E-4 54-55 7.700808199820571E-4 0.0 0.0 0.5983527971260584 7.700808199820571E-4 56-57 7.700808199820571E-4 0.0 0.0 0.8047344568812497 7.700808199820571E-4 58-59 7.700808199820571E-4 0.0 0.0 1.0802308702298307 7.700808199820571E-4 60-61 7.700808199820571E-4 0.0 0.0 1.370551339363066 9.626010249775714E-4 62-63 7.700808199820571E-4 0.0 0.0 1.7754213304686326 0.0011551212299730858 64-65 7.700808199820571E-4 0.0 0.0 2.0838386988714466 0.0011551212299730858 66-67 7.700808199820571E-4 0.0 0.0 2.4771574776772822 0.0011551212299730858 68-69 7.700808199820571E-4 0.0 0.0 2.963848555905942 0.0011551212299730858 70-71 7.700808199820571E-4 0.0 0.0 3.385660325051114 0.0011551212299730858 72-73 7.700808199820571E-4 0.0 0.0 4.009810829646572 0.0023102424599461715 74-75 7.700808199820571E-4 0.0 0.0 4.724253310384925 0.0023102424599461715 76-77 7.700808199820571E-4 0.0 0.0 5.321643506486005 0.0023102424599461715 78-79 7.700808199820571E-4 0.0 0.0 5.906327369057383 0.0023102424599461715 80-81 7.700808199820571E-4 0.0 0.0 6.647915198700103 0.0023102424599461715 82-83 7.700808199820571E-4 0.0 0.0 7.345608421603847 0.0023102424599461715 84-85 7.700808199820571E-4 0.0 0.0 8.147840115820156 0.0023102424599461715 86-87 7.700808199820571E-4 0.0 0.0 9.391905680501168 0.0023102424599461715 88-89 7.700808199820571E-4 0.0 0.0 10.800383500248351 0.0023102424599461715 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 70 4.2874217E-6 40.727905 1 GTCATAG 75 3.0659142E-4 31.67726 1 CTGGTCG 60 0.0039426307 31.671162 9 GTGTAGG 95 3.4726138E-5 30.010036 1 GGTAAGG 95 3.4817596E-5 29.998482 3 TAAGGTG 95 3.4817596E-5 29.998482 5 ACTATAC 65 0.0058372994 29.22929 3 TAGGACA 100 4.9402628E-5 28.498558 4 TAGTCGG 35 0.008381605 27.14671 40-41 GTAAGGT 110 9.452217E-5 25.90778 4 GTACATG 1580 0.0 25.261612 1 GTATCAA 2805 0.0 25.24017 1 GTATATG 170 1.11223926E-7 25.155472 1 GTATACA 95 0.0012216134 25.008364 1 TGTAGGG 95 0.0012243604 24.998735 2 GCTACAC 95 0.0012243604 24.998735 3 AGTACTT 1710 0.0 24.72573 12-13 TACATGG 1625 0.0 24.260311 2 ACATGGG 1605 0.0 23.970749 3 GCCTATG 100 0.0016470015 23.757946 1 >>END_MODULE