FastQCFastQC Report
Thu 26 May 2016
SRR937319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802207
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.41173911471727376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23950.29855137140413884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15960.19895114353277896No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12630.15744066057763145No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9370.11680277035727687No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8140.10147006944591609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46550.031.858731
GGTATCA30650.030.7063561
GTACATG34350.028.920991
TACATGG34850.027.2665862
ATCGACG350.00841544727.12769564-65
ATCAACG55400.026.5861533
CCATGCG908.935209E-426.3939239
CAACGCA55950.026.326455
TATCAAC56150.026.3156572
TCAACGC56450.026.0916374
ACATGGG34650.026.0527733
AACGCAG57550.025.5913286
GAGTACT30150.024.41888612-13
ACGCAGA63200.023.0779767
CGCAGAG63100.022.8886768
GTACTTT32150.022.52977214-15
AGTACTT32650.021.82174712-13
CATGGGA21100.021.6168484
GCAGAGT66000.021.2351119
AGAGTAC50850.020.92697110-11
Produced by FastQC (version 0.11.5)