FastQCFastQC Report
Thu 26 May 2016
SRR937319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802207
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.5100927815389295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.4124870513470962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22400.2792296751337248No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16220.2021922022620097No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.10358922323041311No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG600.003951262731.6650052
CCATGCG650.00584275329.2310569
GGTATCA45400.025.2323071
GTATCAA62650.025.1132351
GTACATG37500.025.0974561
TACATGG37800.023.7487532
TAGGCCC1401.779335E-523.7487535
ACATGGG37400.023.3677583
CCTAGCC2704.0017767E-1122.8691713
TAAGGTG4050.022.2827825
GAGTACT38450.022.17512112-13
AGTACTT39400.021.3993212-13
GTACTTT40000.021.19708614-15
TATCAAC74300.021.1597232
CCCATAC1353.7959105E-421.1100043
ATCAACG74650.021.0605143
TCAACGC75050.020.8849814
GGACCGA2056.827395E-720.8525646
TAGACTG2751.2587407E-920.7261855
CAACGCA75550.020.6838915
Produced by FastQC (version 0.11.5)