##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937319_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 802207 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56656324365158 34.0 31.0 34.0 31.0 34.0 2 32.768006262722714 34.0 31.0 34.0 31.0 34.0 3 32.83356166176561 34.0 31.0 34.0 31.0 34.0 4 36.362157148965295 37.0 37.0 37.0 35.0 37.0 5 36.320528242710424 37.0 37.0 37.0 35.0 37.0 6 36.37358188098583 37.0 37.0 37.0 35.0 37.0 7 36.32687199189237 37.0 37.0 37.0 35.0 37.0 8 36.309686901261145 37.0 37.0 37.0 35.0 37.0 9 38.07392605649165 39.0 38.0 39.0 35.0 39.0 10-11 38.004285053608356 39.0 38.0 39.0 35.0 39.0 12-13 37.92165488458714 39.0 38.0 39.0 35.0 39.0 14-15 39.23643087133371 40.0 38.5 41.0 36.0 41.0 16-17 39.20188367840221 40.0 38.5 41.0 36.0 41.0 18-19 39.19647235688544 40.0 38.5 41.0 36.0 41.0 20-21 39.10045038250726 40.0 38.5 41.0 36.0 41.0 22-23 39.04666189649305 40.0 38.5 41.0 36.0 41.0 24-25 39.0706544570167 40.0 38.0 41.0 36.0 41.0 26-27 38.93713343314132 40.0 38.0 41.0 35.0 41.0 28-29 38.7924008391849 40.0 38.0 41.0 35.0 41.0 30-31 38.8461918183212 40.0 38.0 41.0 35.0 41.0 32-33 38.74236263208873 40.0 38.0 41.0 35.0 41.0 34-35 38.61678282538048 40.0 38.0 41.0 35.0 41.0 36-37 38.45780453174804 40.0 38.0 41.0 35.0 41.0 38-39 38.34357653323893 40.0 38.0 41.0 35.0 41.0 40-41 38.16289561173114 40.0 38.0 41.0 34.0 41.0 42-43 37.948846120764344 40.0 38.0 41.0 34.0 41.0 44-45 37.62402970804294 40.0 37.5 41.0 33.0 41.0 46-47 37.223179927375355 40.0 37.0 41.0 33.0 41.0 48-49 36.86633936128705 40.0 37.0 41.0 31.5 41.0 50-51 36.60448737046673 40.0 37.0 41.0 31.0 41.0 52-53 36.37716075775953 39.5 36.0 41.0 31.0 41.0 54-55 36.16305704138708 39.0 36.0 41.0 30.0 41.0 56-57 35.864925137776154 39.0 35.0 40.0 29.5 41.0 58-59 35.62001141849922 39.0 35.0 40.0 29.0 41.0 60-61 35.277546817716626 38.0 35.0 40.0 28.0 41.0 62-63 34.91985485043138 38.0 34.5 40.0 28.0 41.0 64-65 34.52101016321224 37.0 34.0 40.0 27.0 41.0 66-67 34.10335362319202 37.0 34.0 39.0 26.0 41.0 68-69 33.65480543051856 36.0 33.5 39.0 26.0 40.0 70-71 33.2101222003797 35.5 33.0 39.0 25.0 40.0 72-73 32.750672208046055 35.0 33.0 38.0 24.5 40.0 74-75 32.28386064943338 35.0 32.0 37.0 23.5 39.0 76-77 30.98978131579505 34.0 30.5 36.0 21.5 38.5 78-79 31.592933619377543 35.0 32.0 36.0 22.0 38.5 80-81 31.412006502062436 35.0 32.0 36.0 21.5 37.5 82-83 31.03675048958685 34.5 31.5 35.5 20.0 37.0 84-85 30.652308568736 34.0 31.0 35.0 18.5 36.5 86-87 30.337189777700765 34.0 31.0 35.0 17.5 36.0 88-89 29.960864215844538 34.0 30.5 35.0 9.5 36.0 90-91 29.492151028350538 34.0 30.0 35.0 4.5 35.0 92-93 29.05258617788177 34.0 29.0 35.0 2.0 35.0 94-95 28.64533094326028 34.0 29.0 35.0 2.0 35.0 96-97 28.084505620120492 34.0 29.0 35.0 2.0 35.0 98-99 27.247808857314883 33.0 27.5 35.0 2.0 35.0 100-101 24.357088631737195 31.0 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10201445489450833 1101 2 0.12829222506173465 1101 3 0.01666143127980746 1101 4 0.05625818360380208 1101 5 0.05500782503376911 1101 6 0.11333141115058964 1101 7 0.13378040061493124 1101 8 0.09866322361965274 1101 9 0.10659560742228535 1101 10-11 0.0990257764902438 1101 12-13 0.15367115413067722 1101 14-15 0.1767858380200522 1101 16-17 0.1312720712069151 1101 18-19 0.14653894702509263 1101 20-21 0.13850553426227918 1101 22-23 0.21176253146245472 1101 24-25 0.11392810790482599 1101 26-27 -0.03908473168685589 1101 28-29 -0.09052655352015648 1101 30-31 0.15626664100233967 1101 32-33 0.16829230947271157 1101 34-35 0.15993258583328185 1101 36-37 0.23456087284335325 1101 38-39 0.07845826091328689 1101 40-41 0.033355409687970905 1101 42-43 0.08901022412491244 1101 44-45 0.003423395013690822 1101 46-47 0.013859704613004453 1101 48-49 0.009180483076519863 1101 50-51 0.0012269670503499697 1101 52-53 -0.2397135277123681 1101 54-55 -0.3295198606692935 1101 56-57 -0.19725867561721344 1101 58-59 -0.047692448108122676 1101 60-61 -0.02737546341507624 1101 62-63 0.18013195584324393 1101 64-65 0.1291731865787895 1101 66-67 -0.148430755984009 1101 68-69 -0.043520844382015866 1101 70-71 0.0022972055261902824 1101 72-73 -0.020924451579340086 1101 74-75 0.0669590750372393 1101 76-77 -0.02002302430098979 1101 78-79 0.11933224534260134 1101 80-81 0.019649571492539764 1101 82-83 -0.1963389501937165 1101 84-85 0.01693465520268944 1101 86-87 0.010032347334419711 1101 88-89 0.33835687027901784 1101 90-91 0.4725292733307178 1101 92-93 0.3019887919149049 1101 94-95 0.37705600284681395 1101 96-97 0.6354438014345298 1101 98-99 0.6735323136274118 1101 100-101 1.3303751281082583 1102 1 0.18158904525169817 1102 2 0.17658181895540537 1102 3 0.15749641006090087 1102 4 0.09274291777803256 1102 5 0.12867906457167066 1102 6 0.08555342871513005 1102 7 0.07020103600335403 1102 8 0.09924158622722246 1102 9 0.20986464549843475 1102 10-11 0.11438461917561682 1102 12-13 0.15568713462106842 1102 14-15 0.16736257973877855 1102 16-17 0.1149204341091874 1102 18-19 0.20740976955250545 1102 20-21 0.22629692586902195 1102 22-23 0.24438384025651771 1102 24-25 0.08869197387205219 1102 26-27 0.24791205600617872 1102 28-29 0.32436573104897803 1102 30-31 0.2751312083723718 1102 32-33 0.36514189098868854 1102 34-35 0.39745174179225984 1102 36-37 0.4550901667252347 1102 38-39 0.3035674867178457 1102 40-41 0.48110568965967815 1102 42-43 0.469684256012215 1102 44-45 0.47674227542156444 1102 46-47 0.5368919824038798 1102 48-49 0.3264455278719893 1102 50-51 0.5194306538356841 1102 52-53 0.5276207732424893 1102 54-55 0.6782871135733117 1102 56-57 0.6030367682116164 1102 58-59 0.745572390392411 1102 60-61 0.646487888288334 1102 62-63 0.6048103567627265 1102 64-65 0.6548854567072837 1102 66-67 0.7585633793454747 1102 68-69 0.686776146658886 1102 70-71 0.7823306179392517 1102 72-73 0.8006285809563138 1102 74-75 0.5569408768762045 1102 76-77 0.7573626293592746 1102 78-79 0.6255514852930908 1102 80-81 0.5695572913440081 1102 82-83 0.7157996652725096 1102 84-85 0.9279441742407641 1102 86-87 0.7431327392948113 1102 88-89 1.0342053687465445 1102 90-91 1.3428609292896958 1102 92-93 1.1941444267139154 1102 94-95 1.1009642973770504 1102 96-97 1.3713678554248645 1102 98-99 1.5763351619711585 1102 100-101 1.899834819549966 1103 1 0.23648843679496423 1103 2 0.21023509836490462 1103 3 0.16732602403998698 1103 4 0.09019483518751059 1103 5 0.07105703317856893 1103 6 0.0438319767614459 1103 7 0.10931773093167152 1103 8 0.1579109611762135 1103 9 0.049893118734502195 1103 10-11 0.1418354484359483 1103 12-13 0.13963693467366056 1103 14-15 0.2805754307802317 1103 16-17 0.22159305536981577 1103 18-19 0.3077733927264532 1103 20-21 0.3569020274749448 1103 22-23 0.24523962422263423 1103 24-25 0.16971147894554406 1103 26-27 0.2591335941049948 1103 28-29 0.10333656865179108 1103 30-31 0.0959583830837829 1103 32-33 0.041595476893526495 1103 34-35 0.14126742746223186 1103 36-37 0.09768304478905776 1103 38-39 0.0802611455286737 1103 40-41 0.054777921000187746 1103 42-43 0.06069433045974648 1103 44-45 0.0552828407150443 1103 46-47 0.3096388335722722 1103 48-49 0.5109480170131704 1103 50-51 0.3782773064168694 1103 52-53 0.5049838704776803 1103 54-55 0.5246585895569567 1103 56-57 0.5667401931610669 1103 58-59 0.4499744070954961 1103 60-61 0.5591276474446474 1103 62-63 0.5153270915898531 1103 64-65 0.4533035598819595 1103 66-67 0.5312920946947202 1103 68-69 0.5860747438532741 1103 70-71 0.6090916059786764 1103 72-73 0.6443046208188434 1103 74-75 0.8587435772996841 1103 76-77 0.657861591083627 1103 78-79 0.6492742702294869 1103 80-81 0.813802569230095 1103 82-83 0.5240371810279996 1103 84-85 0.620582845247938 1103 86-87 0.6768168495968645 1103 88-89 0.7568735557993804 1103 90-91 0.7376947145071888 1103 92-93 0.8487930905574359 1103 94-95 1.0601371680051876 1103 96-97 1.5066259281313634 1103 98-99 1.4377786371568249 1103 100-101 1.774485705031335 1104 1 0.17254464065302244 1104 2 0.21386691546420877 1104 3 0.12074747359146443 1104 4 0.0982473476904886 1104 5 0.09085341326906615 1104 6 0.05820047719805643 1104 7 0.1703838577872716 1104 8 0.09714748574555898 1104 9 0.18389823900123048 1104 10-11 0.05119134666568215 1104 12-13 0.11347991316060302 1104 14-15 0.021374331312102868 1104 16-17 0.18795905521104572 1104 18-19 0.17366904248432746 1104 20-21 0.27658280464411433 1104 22-23 0.19224337511570866 1104 24-25 0.055281952074068386 1104 26-27 0.16199240969601192 1104 28-29 0.17537256928685707 1104 30-31 0.2829275027154452 1104 32-33 0.17722548800728788 1104 34-35 0.06437660028115744 1104 36-37 0.06866819998473517 1104 38-39 0.1519473605004933 1104 40-41 0.214647334352577 1104 42-43 0.19871578398043255 1104 44-45 0.2717654357773611 1104 46-47 0.15491201369453478 1104 48-49 0.1161367524263639 1104 50-51 0.32067678645179143 1104 52-53 0.39010780871694806 1104 54-55 0.2116872471738489 1104 56-57 0.1882224905334553 1104 58-59 0.18029710297546586 1104 60-61 0.1879480014972046 1104 62-63 0.2082376059980362 1104 64-65 0.2832705163001563 1104 66-67 0.40742579716356175 1104 68-69 0.47368439297608234 1104 70-71 0.45409190366861196 1104 72-73 0.5161143771100214 1104 74-75 0.5280497613117419 1104 76-77 0.5388005051107747 1104 78-79 0.5638546266635522 1104 80-81 0.6794228558062656 1104 82-83 0.5799929641509642 1104 84-85 0.4330530762553195 1104 86-87 0.6433366558999545 1104 88-89 0.7483397779157563 1104 90-91 1.0476147351470217 1104 92-93 1.109522331646584 1104 94-95 0.9652509847869588 1104 96-97 1.0664938530341175 1104 98-99 1.089352424566524 1104 100-101 1.8899323391505725 1105 1 0.18946193388610766 1105 2 0.06849315047322335 1105 3 -0.06645089422890749 1105 4 0.0339996934308715 1105 5 0.047612563210037706 1105 6 0.028953806803066584 1105 7 -0.12215534145049389 1105 8 -0.030510243287771743 1105 9 0.1286218766076459 1105 10-11 0.05173570860358012 1105 12-13 0.08193928473001932 1105 14-15 0.06160856876523724 1105 16-17 -0.0020135519266304414 1105 18-19 0.046355666068080836 1105 20-21 0.19341711666383077 1105 22-23 -0.03966063594499758 1105 24-25 0.04426072648944768 1105 26-27 0.08389038811312588 1105 28-29 0.1280758667868085 1105 30-31 -0.04766136615094041 1105 32-33 -0.03631391318163679 1105 34-35 -0.1673384052077651 1105 36-37 -0.29394805138749547 1105 38-39 -0.149308749816619 1105 40-41 -0.13798786935918628 1105 42-43 -0.38043806655499424 1105 44-45 -0.06446706178886785 1105 46-47 -0.303676994984194 1105 48-49 -0.04017732822982367 1105 50-51 -0.022204564653243608 1105 52-53 -0.14283288495939672 1105 54-55 0.0556463184056426 1105 56-57 0.20629582469530305 1105 58-59 0.1053965250844584 1105 60-61 0.006761564329906378 1105 62-63 0.1145186754070977 1105 64-65 0.06369717696006916 1105 66-67 0.3112821581072467 1105 68-69 0.4278693129205706 1105 70-71 0.4415806410078247 1105 72-73 0.32610811144586904 1105 74-75 0.35321701829717966 1105 76-77 0.34495457831030407 1105 78-79 0.5715799057704132 1105 80-81 0.5643721867783249 1105 82-83 0.5633915432433234 1105 84-85 0.49800755957105025 1105 86-87 0.5376178133390077 1105 88-89 0.7447072461731814 1105 90-91 0.7270082084998251 1105 92-93 0.7961051367999623 1105 94-95 0.9338443431829475 1105 96-97 0.9297502636888559 1105 98-99 0.8450899087729091 1105 100-101 1.3611937830495755 1106 1 0.010944864958077005 1106 2 0.015797541115098568 1106 3 -0.02342860587661022 1106 4 0.037679976065078336 1106 5 -0.07562299464569833 1106 6 -0.06524741193115347 1106 7 -0.07839448075313982 1106 8 -0.022209840217755072 1106 9 0.003618057955009135 1106 10-11 -0.14023649519925385 1106 12-13 -0.28512218569384373 1106 14-15 -0.10448934328535131 1106 16-17 -0.08352516332616489 1106 18-19 -0.1551631693237283 1106 20-21 -0.2127051269428435 1106 22-23 0.008475951419484318 1106 24-25 -0.07913835954973791 1106 26-27 -0.03959675156330178 1106 28-29 0.0728581496761862 1106 30-31 0.07768837750516155 1106 32-33 0.15729087693623 1106 34-35 -0.24535213079310125 1106 36-37 0.06211929347188061 1106 38-39 0.2849106906294665 1106 40-41 0.06990047862878868 1106 42-43 0.2840443308041145 1106 44-45 0.34403622404941103 1106 46-47 0.5166765134141471 1106 48-49 0.5887044444875684 1106 50-51 0.575395704179904 1106 52-53 0.6406710747587567 1106 54-55 0.6926613719835828 1106 56-57 0.5383236280805193 1106 58-59 0.521711426455397 1106 60-61 0.5332576225818784 1106 62-63 0.886978193196633 1106 64-65 0.52464628734878 1106 66-67 0.44446867547807045 1106 68-69 0.6895755048882819 1106 70-71 0.8337958009689288 1106 72-73 0.7391326820046871 1106 74-75 0.6730269302615213 1106 76-77 0.5718103985658765 1106 78-79 0.4526508895811183 1106 80-81 0.3343410445429491 1106 82-83 0.4386006706317822 1106 84-85 0.6920202716410451 1106 86-87 0.6818290054954055 1106 88-89 0.5839662298904464 1106 90-91 0.3062214402659862 1106 92-93 0.34793907842219696 1106 94-95 0.22064582176524894 1106 96-97 0.43513770867779655 1106 98-99 0.544871931095404 1106 100-101 1.0397485892507063 1107 1 0.02996124040483039 1107 2 0.19156601758845682 1107 3 0.17169401470152224 1107 4 0.1066375586547963 1107 5 0.07785063934396419 1107 6 0.030558556863240938 1107 7 0.05082525253627779 1107 8 0.12223903744033038 1107 9 0.11678111366362032 1107 10-11 0.1216397674749885 1107 12-13 0.1812160009528938 1107 14-15 0.18979371107218412 1107 16-17 0.3403713359127991 1107 18-19 0.12600285030485026 1107 20-21 0.30476384583933225 1107 22-23 0.21290409852618097 1107 24-25 0.09982519732311346 1107 26-27 0.1270065834799894 1107 28-29 0.12700985424682898 1107 30-31 0.24302191349968183 1107 32-33 0.2557219415197096 1107 34-35 0.2299232034395473 1107 36-37 0.2798819138811268 1107 38-39 0.16431672444434042 1107 40-41 0.15203631651672112 1107 42-43 0.0449811743982238 1107 44-45 0.1074704446587944 1107 46-47 0.21500688071645158 1107 48-49 0.30429373468783893 1107 50-51 0.24850530799837856 1107 52-53 0.13015802527776543 1107 54-55 0.09287078855155784 1107 56-57 -0.025991746515479974 1107 58-59 0.06564457359375808 1107 60-61 0.09028880496217795 1107 62-63 -0.15622558474581183 1107 64-65 -0.11733582357867078 1107 66-67 -0.24228783765163087 1107 68-69 -0.25373108138944644 1107 70-71 -0.0785131222456954 1107 72-73 0.011961093415351343 1107 74-75 -0.08094237923378955 1107 76-77 -0.10171613843985128 1107 78-79 0.09831045677280414 1107 80-81 -0.0014168344387677223 1107 82-83 0.16750543464759815 1107 84-85 0.34083375367703184 1107 86-87 0.45074352258121664 1107 88-89 0.451003597659291 1107 90-91 0.654874693294822 1107 92-93 0.60134579199649 1107 94-95 0.34161133347421213 1107 96-97 0.4416999203869665 1107 98-99 0.734843579638671 1107 100-101 1.1991025644711506 1108 1 0.2293084510053518 1108 2 0.21843411859600792 1108 3 0.12451722855739433 1108 4 -0.03890467492804106 1108 5 0.05056415707071693 1108 6 0.03485699452443214 1108 7 0.12695918039130305 1108 8 0.17934808020691406 1108 9 0.17141998758757637 1108 10-11 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-0.8445177454541977 2316 72-73 -0.8137014123636561 2316 74-75 -0.8139502386882533 2316 76-77 -1.0243661615558928 2316 78-79 -1.0456322154417101 2316 80-81 -1.2073271441937372 2316 82-83 -0.9711649305858749 2316 84-85 -0.8184820114639777 2316 86-87 -0.5023475546263647 2316 88-89 -0.7782742571719616 2316 90-91 -0.5564817560810482 2316 92-93 -0.42302591396745015 2316 94-95 -0.1733937520551443 2316 96-97 -0.17358070836938921 2316 98-99 -0.15643704911768452 2316 100-101 0.4970332163435529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 4.0 5 11.0 6 24.0 7 44.0 8 110.0 9 245.0 10 476.0 11 796.0 12 1392.0 13 2124.0 14 2555.0 15 3814.0 16 5807.0 17 9126.0 18 8193.0 19 4311.0 20 3471.0 21 3241.0 22 3303.0 23 3391.0 24 3839.0 25 4498.0 26 5368.0 27 6663.0 28 8228.0 29 10307.0 30 13275.0 31 17236.0 32 23011.0 33 32607.0 34 48253.0 35 75317.0 36 125671.0 37 200531.0 38 161276.0 39 13687.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.181662291340594 20.010351127711715 15.47194283184406 21.33604374910363 2 15.499634757157137 21.321456084846037 39.98760916772001 23.19129999027681 3 19.188937518620506 25.506633574626004 30.695568600124407 24.608860306629087 4 12.899413742338325 17.49673089364715 37.70548000703061 31.89837535698392 5 13.167739337798537 38.39526902791438 34.2405849890863 14.19640664520078 6 31.263127846054694 37.331262379909425 17.302392025998277 14.103217748037602 7 27.789710137159112 33.869811657091 20.887127636632442 17.453350569117447 8 26.38471117803759 35.123976729198326 19.65826775383411 18.833044338929977 9 26.805674844522677 16.758268127802424 20.526123556638122 35.90993347103677 10-11 24.720427520577605 27.39617081376752 27.266341480440836 20.61706018521404 12-13 25.76271334344758 24.931217735913506 27.939561364251176 21.36650755638774 14-15 22.948503316475673 25.98955132528138 26.67977217850256 24.38217317974039 16-17 21.524805941608587 29.342738220933 27.317637467636157 21.81481836982225 18-19 21.389616395768172 27.68792842745077 30.39203098452145 20.530424192259602 20-21 22.406506071706154 26.567789136061936 30.395117454464543 20.630587337767363 22-23 22.08383923251251 26.44414997718779 30.297107212397872 21.174903577901823 24-25 21.88449336516227 26.564032884362476 30.52517747957193 21.026296270903323 26-27 21.556552557209514 26.838389743986095 30.599563939899 21.005493758905384 28-29 21.47391726301893 27.10702302571093 30.621683598894744 20.797376112375392 30-31 21.63564380132734 26.901075038269568 30.288866672317766 21.174414488085326 32-33 21.71505003714766 26.86517394577938 30.059311596551503 21.360464420521463 34-35 22.00311141638006 26.85397908519871 30.149637188406487 20.993272310014746 36-37 22.258358664508453 26.564481838529108 30.193410300215717 20.98374919674672 38-39 21.742371499572116 27.0303556881622 30.083999673400395 21.14327313886529 40-41 21.96440569578675 27.097058489891012 29.907679688658913 21.030856125663327 42-43 21.784962269161195 27.32023153383059 29.463839357509826 21.430966839498385 44-45 22.079650264831898 27.063401341549003 29.506037718444244 21.350910675174863 46-47 22.40543899517207 27.719404093955802 28.741085530293304 21.134071380578828 48-49 22.414912859149823 27.642615933294024 28.5203195683907 21.422151639165453 50-51 22.321732420684437 27.975634717722482 28.386875208019873 21.315757653573204 52-53 22.381567351070235 28.061772086257037 28.049244147707515 21.507416414965213 54-55 22.24070595245367 28.127465853576446 27.946278205001953 21.685549988967935 56-57 22.26133653782627 28.222578461668874 28.077728067693254 21.438356932811605 58-59 22.344357503736568 27.685746945613786 28.084459497361653 21.88543605328799 60-61 22.138425618325446 27.90271089631479 28.597357041262416 21.361506444097348 62-63 21.920464418784675 27.745644204052073 28.830339301452117 21.503552075711134 64-65 21.87776970283231 27.940107727805913 28.51826274265869 21.66385982670308 66-67 21.91117753896438 27.82573575149556 28.22039697983189 22.04268972970817 68-69 21.98559723363172 27.787902623637038 28.393045685215913 21.83345445751533 70-71 22.038950046559055 27.795381989935265 28.24651243382319 21.919155529682488 72-73 21.967202897015103 27.526193429360323 28.35135657344444 22.155247100180127 74-75 21.914231613536156 28.15769495903177 27.780111617076393 22.14796181035568 76-77 21.943338814046747 27.697900915848408 28.13108088062059 22.22767938948426 78-79 21.983976704267103 27.60066915397148 28.043759279088814 22.371594862672602 80-81 22.076658518312605 27.933002329822603 27.924276402474675 22.06606274939012 82-83 21.82508097987361 28.052973957421134 27.819243323562354 22.3027017391429 84-85 21.73661021195914 27.909335065223228 27.856605499955435 22.497449222862194 86-87 21.600908493693023 27.849856707807337 27.91062655898041 22.638608239519225 88-89 21.677135701882435 27.491345749912426 27.786593734534854 23.044924813670285 90-91 21.601634160283098 27.29700937946665 27.9374336527559 23.163922807494348 92-93 21.604907492127918 27.223130832383863 27.985158351637118 23.186803323851098 94-95 21.306720085962848 27.533043217025032 27.92639555625917 23.233841140752947 96-97 21.221891606530484 27.492654639014617 27.89417195312432 23.391281801330578 98-99 21.145569671535963 27.99931688156434 27.325360709595508 23.52975273730419 100-101 21.049016087689377 28.191045051514518 27.248399210077494 23.51153965071861 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 82.0 2 73.0 3 103.5 4 107.5 5 112.0 6 180.0 7 397.0 8 642.0 9 806.5 10 868.5 11 894.0 12 1002.5 13 1114.0 14 1240.0 15 1361.5 16 1400.0 17 1456.0 18 1589.5 19 1668.5 20 1729.0 21 1925.5 22 2185.0 23 2532.5 24 3021.0 25 3702.5 26 4722.5 27 6173.5 28 7401.5 29 8669.5 30 10336.0 31 12191.0 32 13910.5 33 15251.0 34 17019.5 35 18719.5 36 20913.0 37 22767.5 38 24052.0 39 25017.0 40 25467.0 41 26585.5 42 27185.0 43 28009.5 44 29656.0 45 30077.0 46 30074.0 47 31491.5 48 33317.5 49 32113.0 50 29504.5 51 27997.5 52 26871.0 53 26152.0 54 25198.0 55 23193.5 56 19877.5 57 17360.0 58 16039.0 59 14115.5 60 11187.0 61 8664.0 62 6951.0 63 5180.0 64 3550.0 65 2424.5 66 1748.0 67 1362.0 68 1005.0 69 727.0 70 505.5 71 327.5 72 257.0 73 195.5 74 144.0 75 106.5 76 67.5 77 46.5 78 34.0 79 20.5 80 10.5 81 6.0 82 3.0 83 2.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045125509999289465 2 1.2465610497041288E-4 3 0.0 4 0.0 5 4.986244198816515E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0047369319888756894 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.1164026242603215E-4 22-23 6.232805248520643E-4 24-25 2.4931220994082575E-4 26-27 0.002991746519289909 28-29 0.004799260041360896 30-31 3.739683149112386E-4 32-33 3.739683149112386E-4 34-35 0.0 36-37 6.232805248520644E-5 38-39 1.869841574556193E-4 40-41 0.0 42-43 0.009723176187692204 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.4931220994082575E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.869841574556193E-4 84-85 6.232805248520644E-5 86-87 0.0 88-89 0.0 90-91 0.010284128660059061 92-93 6.232805248520643E-4 94-95 0.0 96-97 0.0 98-99 4.3629636739644505E-4 100-101 0.012839578811952527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 802207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.874821160093305 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95594138587401 42.99899462142333 2 12.671781949974243 14.160671004690183 3 4.300127165284725 7.208065089778288 4 1.9362485669455647 4.327501695982814 5 1.0748937815868522 3.0029748906130878 6 0.6693696115402394 2.24405444008872 7 0.44420865243942886 1.737405530897339 8 0.3037769054553391 1.3578784211906914 9 0.23163765262038538 1.1648441172697064 >10 1.288451783291562 13.04187624059569 >50 0.08215051749250345 3.1871824995936064 >100 0.0396115045628229 3.814019487035563 >500 9.002614661527244E-4 0.33815484824408465 >1k 9.002614661527244E-4 1.416377112596923 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4092 0.5100927815389295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3309 0.4124870513470962 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2240 0.2792296751337248 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1622 0.2021922022620097 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 831 0.10358922323041311 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2465610497041288E-4 0.0 14-15 0.0 0.0 0.0 1.2465610497041288E-4 0.0 16-17 0.0 0.0 0.0 1.869841574556193E-4 0.0 18-19 0.0 0.0 0.0 2.4931220994082575E-4 0.0 20-21 0.0 0.0 0.0 4.986244198816515E-4 0.0 22-23 0.0 0.0 0.0 7.479366298224772E-4 0.0 24-25 0.0 0.0 0.0 9.97248839763303E-4 0.0 26-27 0.0 0.0 0.0 0.0014958732596449544 0.0 28-29 6.232805248520644E-5 0.0 0.0 0.002493122099408257 0.0 30-31 1.2465610497041288E-4 0.0 0.0 0.0047369319888756894 0.0 32-33 1.2465610497041288E-4 0.0 0.0 0.00735471019325436 0.0 34-35 1.2465610497041288E-4 0.0 0.0 0.009785504240177411 0.0 36-37 1.2465610497041288E-4 0.0 0.0 0.012901906864437733 0.0 38-39 1.2465610497041288E-4 0.0 0.0 0.01626762169863888 0.0 40-41 1.2465610497041288E-4 0.0 0.0 0.022438098894674317 0.0 42-43 1.2465610497041288E-4 0.0 0.0 0.03222360313485173 0.0 44-45 1.2465610497041288E-4 0.0 0.0 0.05553429476431893 0.0 46-47 1.2465610497041288E-4 0.0 0.0 0.11194118226343076 0.0 48-49 1.2465610497041288E-4 0.0 0.0 0.15550849095059005 0.0 50-51 1.2465610497041288E-4 0.0 0.0 0.20873664777295636 0.0 52-53 1.2465610497041288E-4 0.0 0.0 0.27742216161165384 0.0 54-55 1.2465610497041288E-4 0.0 0.0 0.3653047156157949 0.0 56-57 1.2465610497041288E-4 0.0 0.0 0.47263362199532044 0.0 58-59 1.2465610497041288E-4 0.0 0.0 0.6290147056807034 0.0 60-61 1.2465610497041288E-4 0.0 0.0 0.821546059807506 0.0 62-63 1.2465610497041288E-4 0.0 0.0 1.059452236143539 0.0 64-65 1.2465610497041288E-4 0.0 0.0 1.2711183023833001 0.0 66-67 1.2465610497041288E-4 0.0 0.0 1.5601334817572021 0.0 68-69 1.2465610497041288E-4 0.0 0.0 1.9092952317793288 0.0 70-71 1.2465610497041288E-4 0.0 0.0 2.210090413072935 0.0 72-73 1.2465610497041288E-4 0.0 0.0 2.708216208534705 0.0 74-75 1.2465610497041288E-4 0.0 0.0 3.1277463298126293 0.0 76-77 1.2465610497041288E-4 0.0 0.0 3.525336976615761 0.0 78-79 1.2465610497041288E-4 0.0 0.0 4.0410392828783595 0.0 80-81 1.2465610497041288E-4 0.0 0.0 4.42797183270652 0.0 82-83 1.2465610497041288E-4 0.0 0.0 4.851179309081072 0.0 84-85 1.869841574556193E-4 0.0 0.0 5.6012350926880465 0.0 86-87 2.4931220994082575E-4 0.0 0.0 6.4296995663214105 0.0 88-89 2.4931220994082575E-4 0.0 0.0 7.425577188930039 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTACG 60 0.0039512627 31.665005 2 CCATGCG 65 0.005842753 29.231056 9 GGTATCA 4540 0.0 25.232307 1 GTATCAA 6265 0.0 25.113235 1 GTACATG 3750 0.0 25.097456 1 TACATGG 3780 0.0 23.748753 2 TAGGCCC 140 1.779335E-5 23.748753 5 ACATGGG 3740 0.0 23.367758 3 CCTAGCC 270 4.0017767E-11 22.869171 3 TAAGGTG 405 0.0 22.282782 5 GAGTACT 3845 0.0 22.175121 12-13 AGTACTT 3940 0.0 21.39932 12-13 GTACTTT 4000 0.0 21.197086 14-15 TATCAAC 7430 0.0 21.159723 2 CCCATAC 135 3.7959105E-4 21.110004 3 ATCAACG 7465 0.0 21.060514 3 TCAACGC 7505 0.0 20.884981 4 GGACCGA 205 6.827395E-7 20.852564 6 TAGACTG 275 1.2587407E-9 20.726185 5 CAACGCA 7555 0.0 20.683891 5 >>END_MODULE