FastQCFastQC Report
Thu 26 May 2016
SRR937318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774880
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.41993599008878796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.3301156308073508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.21048420400578155No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10410.13434338220111502No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9670.12479351641544498No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8120.10479041916167664No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46900.032.2375951
GGTATCA30750.031.2330631
TATCAAC55000.027.309912
ATCAACG54700.027.2858943
ATACCGT1056.8976195E-527.1459436
CAACGCA55300.027.0775535
TCAACGC55750.026.8589884
AACGCAG56000.026.7217866
GTACATG34750.024.354181
GAGTACT31100.024.13301512-13
TACATGG34150.024.0797122
CGCAGAG62150.023.9246838
ACGCAGA62400.023.904967
TACCGTT1000.00165206423.75277
ACATGGG33650.023.5899643
GCAGAGT65150.022.6042589
GTACTTT33500.022.40480814-15
AGAGTAC50150.020.50831210-11
AGTACTT34050.020.43785912-13
TGGACCG1404.8249404E-420.3726375
Produced by FastQC (version 0.11.5)