##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937318_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 774880 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10687074127607 33.0 31.0 34.0 31.0 34.0 2 32.238186557918645 34.0 31.0 34.0 31.0 34.0 3 32.278765744373324 34.0 31.0 34.0 31.0 34.0 4 35.86334658269668 37.0 35.0 37.0 35.0 37.0 5 35.865952147429276 37.0 35.0 37.0 35.0 37.0 6 35.92479480693785 37.0 36.0 37.0 35.0 37.0 7 35.87770751600248 37.0 36.0 37.0 35.0 37.0 8 35.87260866198637 37.0 36.0 37.0 35.0 37.0 9 37.556448740450136 39.0 38.0 39.0 35.0 39.0 10-11 37.463190429485856 39.0 37.0 39.0 35.0 39.0 12-13 37.34706018996489 39.0 37.0 39.0 35.0 39.0 14-15 38.61528043051827 40.0 38.0 41.0 35.0 41.0 16-17 38.58510672620277 40.0 38.0 41.0 35.0 41.0 18-19 38.566472873219084 40.0 38.0 41.0 34.5 41.0 20-21 38.508585200289076 40.0 38.0 41.0 34.0 41.0 22-23 38.36318462213504 40.0 38.0 41.0 34.0 41.0 24-25 38.22835406772661 40.0 38.0 41.0 34.0 41.0 26-27 38.07371786599215 40.0 38.0 41.0 34.0 41.0 28-29 38.122103422465415 40.0 38.0 41.0 34.0 41.0 30-31 37.96513202044187 40.0 38.0 41.0 34.0 41.0 32-33 37.771194894693366 40.0 37.0 41.0 33.0 41.0 34-35 37.655331793309934 40.0 37.0 41.0 33.0 41.0 36-37 37.71531269357836 40.0 38.0 41.0 33.0 41.0 38-39 37.63814332541813 40.0 37.5 41.0 33.0 41.0 40-41 37.46862417406566 40.0 37.0 41.0 33.0 41.0 42-43 37.24045077947553 40.0 37.0 41.0 32.5 41.0 44-45 37.00532469543671 40.0 37.0 41.0 31.5 41.0 46-47 36.63894344930828 40.0 36.5 41.0 30.5 41.0 48-49 36.31820088271732 40.0 36.0 41.0 30.0 41.0 50-51 35.45000580735082 38.5 35.0 40.0 29.0 40.5 52-53 35.518464794548834 38.5 35.5 40.0 29.0 40.5 54-55 35.83495831612637 39.0 36.0 40.5 29.0 41.0 56-57 35.65137956844931 39.0 35.0 41.0 28.0 41.0 58-59 35.436638576295685 39.0 35.0 40.5 28.0 41.0 60-61 35.15512982655379 38.5 35.0 40.0 27.0 41.0 62-63 34.84143222176337 38.0 34.5 40.0 26.0 41.0 64-65 34.48582361139789 38.0 34.0 40.0 26.0 41.0 66-67 34.11585858455503 37.0 34.0 40.0 25.5 41.0 68-69 33.6579250980797 36.5 34.0 39.0 24.5 41.0 70-71 33.2105267912451 36.0 33.0 39.0 23.0 40.5 72-73 32.81773629465208 35.5 33.0 38.5 22.0 40.0 74-75 32.36782275965311 35.0 32.5 37.5 20.5 39.5 76-77 31.944625619450754 35.0 32.0 37.0 20.0 39.0 78-79 31.483143196365887 35.0 32.0 36.5 18.0 39.0 80-81 31.014317700805286 35.0 31.0 36.0 15.5 38.5 82-83 30.562776171794344 34.5 31.0 35.5 9.5 37.0 84-85 30.148955322114393 34.0 30.5 35.0 7.0 37.0 86-87 29.749299891596117 34.0 30.0 35.0 3.5 36.0 88-89 29.386586955399544 34.0 30.0 35.0 2.0 36.0 90-91 29.020567700805287 34.0 29.0 35.0 2.0 35.5 92-93 28.61675291658063 34.0 29.0 35.0 2.0 35.0 94-95 28.143146035515176 34.0 29.0 35.0 2.0 35.0 96-97 27.5967627245509 34.0 27.5 35.0 2.0 35.0 98-99 26.91709813132356 33.0 26.5 35.0 2.0 35.0 100-101 25.07764557092711 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02401625979398858 1101 2 0.07778584416670498 1101 3 0.18507670107396734 1101 4 0.07946947088970546 1101 5 0.14867812795612423 1101 6 0.18578246407368226 1101 7 0.03754443530104368 1101 8 0.19379413168021387 1101 9 0.18993681400387175 1101 10-11 0.17020514634572237 1101 12-13 -0.01989312730526649 1101 14-15 0.16177103259676784 1101 16-17 0.24199131777918836 1101 18-19 0.2126515426551734 1101 20-21 0.18177572864817648 1101 22-23 0.24279251540257718 1101 24-25 0.21305812961580983 1101 26-27 0.15480338753111766 1101 28-29 0.1416186598320337 1101 30-31 0.11685949935009177 1101 32-33 0.25020492904258873 1101 34-35 0.17978676053457576 1101 36-37 0.3879683858272429 1101 38-39 0.1668648033865736 1101 40-41 0.07623307091944298 1101 42-43 0.3650363306662925 1101 44-45 0.40181613288317664 1101 46-47 0.7439988462159945 1101 48-49 0.6224173455685573 1101 50-51 0.6744830569264408 1101 52-53 0.666985494265596 1101 54-55 0.7728030766074667 1101 56-57 0.7534477659389935 1101 58-59 0.7825838292461711 1101 60-61 0.8811536156719484 1101 62-63 0.8583992287282811 1101 64-65 0.9078098398134458 1101 66-67 0.9356937095290974 1101 68-69 1.0079053826324298 1101 70-71 1.170670125487277 1101 72-73 0.9593345410064984 1101 74-75 0.9192238693385875 1101 76-77 1.0711980839363058 1101 78-79 0.9244752982824167 1101 80-81 0.706003464378238 1101 82-83 0.5698465452229264 1101 84-85 0.5794036980414283 1101 86-87 0.6345809481660254 1101 88-89 0.6547181702167215 1101 90-91 0.7548994326166323 1101 92-93 0.9790587314132964 1101 94-95 1.0480264877679737 1101 96-97 1.0101859896557848 1101 98-99 1.0595881443126522 1101 100-101 1.5713075767512343 1102 1 0.046739626886520114 1102 2 0.019226549588367448 1102 3 0.23004874057081537 1102 4 0.08218100582977428 1102 5 0.04853022605030333 1102 6 -0.00412217441179763 1102 7 0.09056656424333198 1102 8 0.04215101193601356 1102 9 0.11704934622535745 1102 10-11 0.22278568245491215 1102 12-13 0.1826867045091305 1102 14-15 0.04865213355038378 1102 16-17 0.298575065472626 1102 18-19 0.2607175491628624 1102 20-21 0.2724635429397253 1102 22-23 0.23676234341378688 1102 24-25 0.21972427979962816 1102 26-27 0.09637086601491518 1102 28-29 0.14285188000867777 1102 30-31 0.31169842713170937 1102 32-33 0.3703195882343451 1102 34-35 0.19045119223911655 1102 36-37 0.33915864402171536 1102 38-39 0.4535275729554087 1102 40-41 0.3766619864463223 1102 42-43 0.14047897994157665 1102 44-45 0.2918454315464274 1102 46-47 0.3157721196373231 1102 48-49 0.292944721507034 1102 50-51 0.19305742341328624 1102 52-53 0.35596680228187694 1102 54-55 0.18849969458388216 1102 56-57 0.1771353125985229 1102 58-59 0.4288693785256825 1102 60-61 0.35958529203441003 1102 62-63 0.19431826299926058 1102 64-65 0.5396918563502808 1102 66-67 0.4284211615314035 1102 68-69 0.36679710435147683 1102 70-71 0.3296308680956983 1102 72-73 0.3949500242231494 1102 74-75 0.4610857430444426 1102 76-77 0.47070366899398763 1102 78-79 0.3598858317152853 1102 80-81 0.3167009979378861 1102 82-83 0.32095651381137813 1102 84-85 0.4138577991499872 1102 86-87 0.3106912689018735 1102 88-89 0.2591728347611131 1102 90-91 0.3045958815623031 1102 92-93 0.44952131223112346 1102 94-95 0.48353914394534314 1102 96-97 0.5678768887585122 1102 98-99 0.9420117635427161 1102 100-101 1.5033487639105303 1103 1 0.048586927332955554 1103 2 -0.13510620159760123 1103 3 -0.04794431966639934 1103 4 -0.06928789883076547 1103 5 -0.10704202126854767 1103 6 0.020220963415567894 1103 7 -0.04661407307532528 1103 8 0.02367800747170179 1103 9 0.009523720239997147 1103 10-11 0.0556027802468293 1103 12-13 0.13633521136307536 1103 14-15 0.027039691521821396 1103 16-17 -0.02324891369038795 1103 18-19 0.07885090868397526 1103 20-21 0.01860836492472373 1103 22-23 -0.13151841086516924 1103 24-25 -0.12587307594195352 1103 26-27 0.052176768529115236 1103 28-29 -0.13175844399437153 1103 30-31 -0.19270926004459454 1103 32-33 -0.0027647665110421826 1103 34-35 0.07831748944568773 1103 36-37 0.2040575467889525 1103 38-39 0.01601197972246382 1103 40-41 0.16867214659878016 1103 42-43 0.2200699373705035 1103 44-45 0.07841410635607815 1103 46-47 0.2709706601991897 1103 48-49 0.17645287599372494 1103 50-51 0.12079683372807892 1103 52-53 0.18373744329867492 1103 54-55 0.3074121406821675 1103 56-57 0.2536124894138396 1103 58-59 0.3734932741708974 1103 60-61 0.31539429107714056 1103 62-63 0.21678844342956438 1103 64-65 0.3532587210178306 1103 66-67 0.4943325406544332 1103 68-69 0.5243130868622785 1103 70-71 0.5981910177199623 1103 72-73 0.4888336726061482 1103 74-75 0.5211331818614653 1103 76-77 0.5164101994841808 1103 78-79 0.28064522437102823 1103 80-81 0.10190056477778242 1103 82-83 0.15648174697997774 1103 84-85 0.26417514908779083 1103 86-87 0.3858749815157161 1103 88-89 0.3565936034433541 1103 90-91 0.39702814278898657 1103 92-93 0.3946229579917997 1103 94-95 0.48092523150809185 1103 96-97 0.544132265659119 1103 98-99 0.44601734437184604 1103 100-101 0.5747936395097106 1104 1 0.11773389820203306 1104 2 0.161561388760596 1104 3 0.10974810644790267 1104 4 -0.030506324937370266 1104 5 0.02147907161248952 1104 6 -0.020250196240660046 1104 7 0.0847839340601908 1104 8 0.10263631434511922 1104 9 0.26518421741696585 1104 10-11 0.13873742368264175 1104 12-13 -0.0373760465123496 1104 14-15 0.12051823686159935 1104 16-17 0.08257390302996015 1104 18-19 0.11706191061988136 1104 20-21 0.05129838559231814 1104 22-23 0.11258632035983851 1104 24-25 0.040959566547087434 1104 26-27 0.055944660241678434 1104 28-29 -0.07466324218701459 1104 30-31 0.010923349345496547 1104 32-33 -0.04994519810590958 1104 34-35 0.07986948365722668 1104 36-37 0.2780668876195804 1104 38-39 0.22082325799608782 1104 40-41 0.281472202020268 1104 42-43 0.23161848698236298 1104 44-45 0.23711056592340896 1104 46-47 0.3706287981140335 1104 48-49 0.515888346518345 1104 50-51 0.29499302965844976 1104 52-53 0.2856947071703999 1104 54-55 0.373741371285611 1104 56-57 0.2934091618150916 1104 58-59 0.27277577752889925 1104 60-61 0.1548874923225867 1104 62-63 0.06815059978744387 1104 64-65 0.1544273832430818 1104 66-67 0.24870804820368875 1104 68-69 0.16162945506111726 1104 70-71 -0.021797474623021174 1104 72-73 -0.05288014081585857 1104 74-75 -0.06866791656172211 1104 76-77 0.07559236684938853 1104 78-79 0.18786955055729493 1104 80-81 0.17370314880484372 1104 82-83 0.15353170714577757 1104 84-85 0.48990034315923126 1104 86-87 0.318763980734424 1104 88-89 0.11503864572148714 1104 90-91 0.18818629802304798 1104 92-93 0.4003767865007717 1104 94-95 0.3828460288078368 1104 96-97 0.5552762191358518 1104 98-99 0.4617167328110767 1104 100-101 0.761056037182005 1105 1 0.1436050611938171 1105 2 0.08789083104334594 1105 3 0.1763715392191969 1105 4 0.22820962837002412 1105 5 0.1741811900733623 1105 6 0.06898456381122031 1105 7 0.14217260677960297 1105 8 0.2001633355297301 1105 9 0.31946437305899167 1105 10-11 0.12335912709812646 1105 12-13 0.08295901617679391 1105 14-15 0.17333708683998594 1105 16-17 0.12856612092880226 1105 18-19 0.13880639829822172 1105 20-21 0.1347722290856197 1105 22-23 0.041795140074491144 1105 24-25 0.22328122672866613 1105 26-27 0.10812797195806212 1105 28-29 0.0923579424217138 1105 30-31 0.03666264895639415 1105 32-33 0.11956625887636108 1105 34-35 0.08631781915224934 1105 36-37 0.09918448381499445 1105 38-39 0.2631946371573548 1105 40-41 0.17171863522391106 1105 42-43 0.20890772174241334 1105 44-45 0.22370589666270746 1105 46-47 0.36474250325886715 1105 48-49 0.4703219824889402 1105 50-51 0.3009484987462159 1105 52-53 0.5002969553329635 1105 54-55 0.5570725429241747 1105 56-57 0.35125179043593135 1105 58-59 0.5134718432444245 1105 60-61 0.5071334931872613 1105 62-63 0.6171000161298537 1105 64-65 0.7152682825080987 1105 66-67 0.5861442782080033 1105 68-69 0.5363786984679493 1105 70-71 0.5092790493717914 1105 72-73 0.5862767982243469 1105 74-75 0.5214942105459315 1105 76-77 0.5126416534901175 1105 78-79 0.34136068760874494 1105 80-81 0.516165319146392 1105 82-83 0.6049865277251136 1105 84-85 0.7347274075777364 1105 86-87 0.8140385159700507 1105 88-89 0.7999500162359681 1105 90-91 0.9192520135440141 1105 92-93 0.9286387839067345 1105 94-95 1.0431227257382254 1105 96-97 1.1829156484484322 1105 98-99 1.0491594478997044 1105 100-101 1.294759020320786 1106 1 -0.11781256332725576 1106 2 -0.052402910974535644 1106 3 -0.044381972913626555 1106 4 0.026664289928135076 1106 5 -0.049892884000712456 1106 6 0.05761802485847056 1106 7 0.05138078047053796 1106 8 -0.10032197736937576 1106 9 -0.08864546091291459 1106 10-11 -0.2288183952518068 1106 12-13 -0.18218785483215072 1106 14-15 -0.16921719157635806 1106 16-17 -0.23951178456290023 1106 18-19 -0.05480151996486882 1106 20-21 -7.003124214008949E-4 1106 22-23 8.377353553612465E-5 1106 24-25 0.14507560731615143 1106 26-27 0.0101713946911417 1106 28-29 0.0923643281177533 1106 30-31 0.1306153948056945 1106 32-33 0.19807200600279629 1106 34-35 -0.0024625129039392846 1106 36-37 -0.06418716369458366 1106 38-39 0.015487102036026101 1106 40-41 -0.02462874664071535 1106 42-43 0.09282215341546163 1106 44-45 0.1506918481314159 1106 46-47 0.25566204348874777 1106 48-49 0.2422823911734895 1106 50-51 0.2774491373158199 1106 52-53 0.14413475865352154 1106 54-55 0.026778800906292588 1106 56-57 -0.07932292220774428 1106 58-59 0.15229048947686152 1106 60-61 0.08019224159083649 1106 62-63 0.20280244269434888 1106 64-65 0.3842018712692763 1106 66-67 0.38025028076229006 1106 68-69 0.31882442005159817 1106 70-71 0.1716928424752382 1106 72-73 -0.0015839936019617085 1106 74-75 -0.07217756467756686 1106 76-77 0.15239899704056015 1106 78-79 0.03529427636992111 1106 80-81 -0.125871717890643 1106 82-83 -0.02281040714078486 1106 84-85 -0.019340280800296483 1106 86-87 0.09804873308371498 1106 88-89 0.0014825832857745525 1106 90-91 0.18725329068322694 1106 92-93 0.30709344546484374 1106 94-95 0.32550459036147217 1106 96-97 0.08977388599632619 1106 98-99 0.3546128323186082 1106 100-101 0.4551613191713635 1107 1 0.03789624893327925 1107 2 0.051454049434148885 1107 3 -0.007038422466628447 1107 4 0.1844165249217511 1107 5 0.0732233356672296 1107 6 -0.005268309751997435 1107 7 0.12060172555354853 1107 8 0.1485158259511863 1107 9 0.06670575236933729 1107 10-11 0.11728633016326029 1107 12-13 0.13832300085111626 1107 14-15 0.1430688876062689 1107 16-17 0.0859799140322437 1107 18-19 0.023691455769480285 1107 20-21 -0.007852291987177296 1107 22-23 0.08432387570667288 1107 24-25 0.208396169257405 1107 26-27 0.19556092214963172 1107 28-29 0.06536560525696444 1107 30-31 0.2689040282667392 1107 32-33 0.1344704734357478 1107 34-35 0.15894234022504605 1107 36-37 0.17749866006020198 1107 38-39 0.2072367827512238 1107 40-41 0.0429830030399998 1107 42-43 0.11034450335160528 1107 44-45 0.05508520700752939 1107 46-47 0.037194610538556105 1107 48-49 0.26677792118626087 1107 50-51 0.24156152556614074 1107 52-53 0.42663795150154016 1107 54-55 0.48933307932264114 1107 56-57 0.45738205863516157 1107 58-59 0.6103492378804347 1107 60-61 0.6466545659841927 1107 62-63 0.29830691268158205 1107 64-65 0.30563226718243186 1107 66-67 0.4547841249513027 1107 68-69 0.6116468977016609 1107 70-71 0.406635371210875 1107 72-73 0.394610131557684 1107 74-75 0.25454018755743135 1107 76-77 0.2624117684326457 1107 78-79 0.4462265879918874 1107 80-81 0.35767872910807696 1107 82-83 0.4494501292576807 1107 84-85 0.41309905508731504 1107 86-87 0.4180998186520455 1107 88-89 0.4113676407570388 1107 90-91 0.5316466495593453 1107 92-93 0.5305928068952426 1107 94-95 0.8193487793780747 1107 96-97 0.8234903610921123 1107 98-99 0.8642935475165636 1107 100-101 0.6947015490564219 1108 1 0.0257304646055303 1108 2 -0.08202618140478535 1108 3 0.029506624511959956 1108 4 -0.0464929995691179 1108 5 -0.08739096299257199 1108 6 -0.019397178927370362 1108 7 -0.02328151954588975 1108 8 0.003279979144295453 1108 9 0.07942287928305802 1108 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-1.0153913705348359 2316 52-53 -1.170454678653968 2316 54-55 -1.4267183067490308 2316 56-57 -1.3251534383222392 2316 58-59 -1.0698941698274709 2316 60-61 -1.289239055832347 2316 62-63 -1.1799261276609911 2316 64-65 -1.1587513253350323 2316 66-67 -1.2034962446678108 2316 68-69 -1.5103206479372773 2316 70-71 -1.8847078787327831 2316 72-73 -1.8342787573312087 2316 74-75 -1.9941593842253091 2316 76-77 -2.4227831831111892 2316 78-79 -2.447201403894521 2316 80-81 -2.0708608246444555 2316 82-83 -1.8405363480918808 2316 84-85 -2.0632137688928545 2316 86-87 -2.343935340239099 2316 88-89 -2.415409460888217 2316 90-91 -2.7530028884167663 2316 92-93 -2.662668405635749 2316 94-95 -3.108478581451312 2316 96-97 -3.1627052665842434 2316 98-99 -3.253324212231014 2316 100-101 -3.215731175757643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2416.0 3 403.0 4 352.0 5 421.0 6 473.0 7 528.0 8 618.0 9 752.0 10 957.0 11 1370.0 12 1923.0 13 2747.0 14 3083.0 15 4074.0 16 5662.0 17 8006.0 18 6617.0 19 4016.0 20 3758.0 21 3977.0 22 4091.0 23 4543.0 24 4975.0 25 5671.0 26 6442.0 27 7754.0 28 9168.0 29 11124.0 30 13951.0 31 17977.0 32 23446.0 33 32009.0 34 46159.0 35 69630.0 36 112025.0 37 174324.0 38 157285.0 39 22152.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0501957992168 19.215896954594 15.247095375254863 22.486811870934336 2 14.691477041129925 20.246378405657385 41.29995665085825 23.76218790235444 3 18.44990088350836 25.25335520969737 30.727406356912535 25.56933754988173 4 12.09402959193973 16.73119576385456 38.634252010279795 32.54052263392591 5 12.938611247703097 37.62247987784363 35.17029426227386 14.268614612179404 6 30.604383028083504 36.90930181132573 18.16239730712879 14.323917853461976 7 27.11003361779157 33.306309800879234 21.90923196276183 17.674424618567365 8 25.563289494271096 34.559758145958085 20.6834083523952 19.193544007375618 9 25.931117056008606 16.626088215785124 21.137343079057512 36.30545164914876 10-11 24.036790266512167 26.851217203277606 28.331402085747392 20.780590444462838 12-13 25.128844399414728 24.446200229789486 28.625084296015924 21.799871074779862 14-15 22.364292645964483 25.595304671988433 27.193063555412746 24.847339126634335 16-17 21.05580295953781 28.95583451001081 27.896372212826044 22.091990317625335 18-19 21.110435873233428 27.439066964920674 30.462832464862487 20.98766469698341 20-21 21.985527220823013 26.379141821341207 30.44914228678703 21.186188671048754 22-23 21.67078905483315 26.205653080671667 30.444881752323127 21.678676112172056 24-25 21.323817927360995 26.475194978554782 30.564104553108827 21.636882540975392 26-27 21.107485413534224 26.670769278504856 30.541208624276173 21.68053668368475 28-29 21.125016809243533 26.832595450642888 30.593469738189565 21.44891800192401 30-31 21.236074201953976 26.634272819557864 30.36169896352621 21.767954014961948 32-33 21.06875786364806 26.653968451011657 30.249360886650965 22.027912798689318 34-35 21.56015565087958 26.65948072404299 30.22738529541466 21.55297832966277 36-37 21.584576876433488 26.36141142034127 30.335233017265228 21.718778685960007 38-39 21.103891696279483 26.823232652987343 30.179861905257514 21.893013745475656 40-41 21.420597309965487 26.820705521571536 30.194722712607685 21.563974455855288 42-43 21.110943745567596 26.846675850940656 29.879619639175694 22.162760764316058 44-45 21.365718367861493 26.868092616716392 29.780289047423558 21.985899967998552 46-47 21.592643667459942 27.17176477890078 29.327770698413254 21.907820855226028 48-49 21.464825085572485 27.142157008538497 29.152189065084226 22.240828840804788 50-51 21.47805362514128 27.435748887227884 29.04361083882715 22.042586648803688 52-53 21.668487156653324 27.572436423509995 28.582210158916165 22.176866260920512 54-55 21.58574614277004 27.31881541999727 28.758645348209445 22.336793089023242 56-57 21.637844605858614 27.553795326630993 28.59314773361189 22.215212333898506 58-59 21.77373897214724 27.194322918282705 28.717537000837755 22.3144011087323 60-61 21.44647189128708 27.22354265901424 29.272284552440876 22.05770089725781 62-63 21.233718421867493 27.21361262433602 29.41256706080546 22.140101892991026 64-65 21.29737123118014 27.219663925079956 29.12336881623957 22.35959602750033 66-67 21.247578472988458 27.11095425455105 28.90616465672653 22.735302615733964 68-69 21.34792242478146 27.090179510979308 29.17977728538445 22.38212077885478 70-71 21.503240299303457 26.965689997899055 29.01149053767959 22.519579165117896 72-73 21.27508834938716 26.92091639016072 29.067158462633397 22.73683679781872 74-75 21.21533582345237 27.478127420141004 28.697587584054446 22.60894917235218 76-77 21.228123933347813 26.955011843071546 28.864901066393607 22.951963157187038 78-79 21.273484554156287 26.922641815580757 28.893459976301312 22.910413653961644 80-81 21.36806284617891 27.252263900166014 28.703888064294247 22.675785189360834 82-83 21.18943776369876 27.139794037814248 28.854615726223237 22.816152472263752 84-85 21.189241458301957 26.95052055896016 28.75087179971133 23.10936618302655 86-87 21.232219707086518 26.945570940831683 28.88613243805419 22.936076914027606 88-89 21.18637932594728 26.444417152534484 28.749369772342376 23.619833749175857 90-91 21.068012752391073 26.32249173240664 29.080920282041934 23.528575233160353 92-93 21.025653621809457 26.404782712116827 29.212408989368306 23.357154676705417 94-95 20.78250465868921 26.399238838375318 29.158837547968503 23.659418954966974 96-97 20.62861804088661 26.529240363310123 29.262856891697282 23.579284704105984 98-99 20.531406275049257 26.95959538498932 28.778139205353497 23.730859134607925 100-101 20.63494115457009 26.652442240469938 28.916608062547834 23.796008542412146 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 970.0 1 546.0 2 112.5 3 109.0 4 113.5 5 116.5 6 162.0 7 313.5 8 540.5 9 707.5 10 733.5 11 748.0 12 824.0 13 890.5 14 979.5 15 1079.0 16 1164.0 17 1248.5 18 1279.5 19 1389.5 20 1597.5 21 1761.5 22 2031.5 23 2460.0 24 2917.0 25 3602.0 26 4727.5 27 6102.5 28 7281.5 29 8477.5 30 9944.0 31 11735.0 32 13624.0 33 15129.0 34 16653.5 35 18420.5 36 20734.5 37 22796.0 38 23716.0 39 24332.5 40 24945.0 41 25871.5 42 26745.0 43 27743.0 44 29166.0 45 29857.5 46 29980.0 47 30865.5 48 32467.5 49 31781.0 50 28915.5 51 26617.0 52 25230.5 53 24543.5 54 23651.5 55 21421.5 56 18575.5 57 16207.0 58 14576.5 59 12887.5 60 10532.5 61 8333.5 62 6400.0 63 4549.0 64 3163.0 65 2313.5 66 1685.0 67 1221.0 68 881.0 69 608.0 70 418.0 71 289.0 72 211.0 73 167.5 74 131.5 75 88.5 76 59.0 77 43.0 78 28.5 79 21.0 80 16.0 81 10.5 82 10.0 83 9.0 84 6.5 85 6.0 86 5.5 87 4.0 88 4.0 89 4.5 90 2.0 91 1.5 92 2.0 93 1.5 94 1.5 95 2.0 96 2.5 97 6.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3103706380342763 2 0.26907392112327067 3 0.2648151971918233 4 0.2712678092091679 5 0.2710097047284741 6 0.20377348750774313 7 0.19099731571340078 8 0.1962884575676234 9 0.19112636795374768 10-11 0.21061325624612842 12-13 0.2017731777823663 14-15 0.19557867024571546 16-17 0.1956431963658889 18-19 0.19228783811686972 20-21 0.18428659921536236 22-23 0.18854532314680983 24-25 0.18035050588478216 26-27 0.17925356184183358 28-29 0.19306215155895107 30-31 0.2045478009498245 32-33 0.19912760685525502 34-35 0.2079031591988437 36-37 0.18299607681189345 38-39 0.18396396861449515 40-41 0.18009240140408836 42-43 0.18796458806524882 44-45 0.19067468511253355 46-47 0.19428814784224652 48-49 0.2011924427008053 50-51 0.20467685319017137 52-53 0.2240992153623787 54-55 0.20125696882097874 56-57 0.17673704315506916 58-59 0.1786083006400991 60-61 0.17505936403055958 62-63 0.18344775965310758 64-65 0.17576915135246748 66-67 0.17415599834813134 68-69 0.1721556886227545 70-71 0.18293155069172 72-73 0.17918903572166014 74-75 0.18364133801362792 76-77 0.18480280817674996 78-79 0.18318965517241378 80-81 0.18531901713813753 82-83 0.17918903572166014 84-85 0.17247831922362172 86-87 0.17305905430518273 88-89 0.17422052446830477 90-91 0.17783398719801777 92-93 0.17344621102622343 94-95 0.1710587445798059 96-97 0.17112327069997935 98-99 0.18118934544703696 100-101 0.17138137518067315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 774880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.31763633858809 #Duplication Level Percentage of deduplicated Percentage of total 1 78.94089214439406 46.825871324648716 2 11.914994826695233 14.135386602121326 3 3.874482284111018 6.894753933875986 4 1.6914811457584085 4.013386539107023 5 0.910949209149033 2.7017676955613377 6 0.5500964575060857 1.9578252970494938 7 0.3920617329916497 1.6279322709912711 8 0.2706040766734731 1.2841275369485186 9 0.21312109301482285 1.1377655542382101 >10 1.136022314056317 12.20904782324156 >50 0.07651895397538193 3.1750342590146716 >100 0.027031776120800755 2.5465289312880492 >500 8.719927768394095E-4 0.3934945173691095 >1k 8.719927768394095E-4 1.097077714544701 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3254 0.41993599008878796 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2558 0.3301156308073508 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.21048420400578155 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1041 0.13434338220111502 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 967 0.12479351641544498 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 812 0.10479041916167664 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.452612017344621E-5 0.0 18-19 0.0 0.0 0.0 1.9357836052033865E-4 0.0 20-21 0.0 0.0 0.0 2.5810448069378485E-4 0.0 22-23 0.0 0.0 0.0 2.5810448069378485E-4 0.0 24-25 2.5810448069378485E-4 0.0 0.0 4.5168284121412344E-4 0.0 26-27 2.5810448069378485E-4 0.0 0.0 6.452612017344621E-4 0.0 28-29 3.8715672104067724E-4 0.0 0.0 0.0014195746438158167 0.0 30-31 3.8715672104067724E-4 0.0 0.0 0.003548936609539542 0.0 32-33 3.8715672104067724E-4 0.0 0.0 0.005678298575263266 0.0 34-35 3.8715672104067724E-4 0.0 0.0 0.007807660540986992 0.0 36-37 3.8715672104067724E-4 0.0 0.0 0.010646809828618625 0.0 38-39 3.8715672104067724E-4 0.0 0.0 0.013163328515383026 0.0 40-41 3.8715672104067724E-4 0.0 0.0 0.02077741069584968 0.0 42-43 3.8715672104067724E-4 0.0 0.0 0.030391802601693167 0.0 44-45 3.8715672104067724E-4 0.0 0.0 0.04903985133181912 0.0 46-47 4.5168284121412344E-4 0.0 0.0 0.09640202353912863 0.0 48-49 5.162089613875697E-4 0.0 0.0 0.1346014866818088 0.0 50-51 5.162089613875697E-4 0.0 0.0 0.18093124096634317 0.0 52-53 5.162089613875697E-4 0.0 0.0 0.23906927524261823 0.0 54-55 5.162089613875697E-4 0.0 0.0 0.3117902126780921 0.0 56-57 5.162089613875697E-4 0.0 0.0 0.400707206277101 0.0 58-59 5.162089613875697E-4 0.0 0.0 0.5351796407185629 0.0 60-61 5.162089613875697E-4 0.0 0.0 0.7061738591781954 0.0 62-63 5.162089613875697E-4 0.0 0.0 0.9285308692958909 0.0 64-65 5.162089613875697E-4 0.0 0.0 1.1118495767086516 0.0 66-67 5.162089613875697E-4 0.0 0.0 1.3815042329134835 0.0 68-69 5.162089613875697E-4 0.0 0.0 1.689745508982036 0.0 70-71 5.162089613875697E-4 0.0 0.0 1.9568191203799299 0.0 72-73 5.162089613875697E-4 0.0 0.0 2.4339897790625646 0.0 74-75 5.162089613875697E-4 0.0 0.0 2.835213194301053 0.0 76-77 5.162089613875697E-4 0.0 0.0 3.195720627710097 0.0 78-79 5.162089613875697E-4 0.0 0.0 3.671342659508569 0.0 80-81 7.097873219079082E-4 0.0 0.0 4.035399029527152 0.0 82-83 7.743134420813545E-4 0.0 0.0 4.460174478628949 0.0 84-85 7.743134420813545E-4 0.0 0.0 5.206677162915549 0.0 86-87 7.743134420813545E-4 0.0 0.0 6.03280507949618 0.0 88-89 7.743134420813545E-4 0.0 0.0 7.072708032211439 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4690 0.0 32.237595 1 GGTATCA 3075 0.0 31.233063 1 TATCAAC 5500 0.0 27.30991 2 ATCAACG 5470 0.0 27.285894 3 ATACCGT 105 6.8976195E-5 27.145943 6 CAACGCA 5530 0.0 27.077553 5 TCAACGC 5575 0.0 26.858988 4 AACGCAG 5600 0.0 26.721786 6 GTACATG 3475 0.0 24.35418 1 GAGTACT 3110 0.0 24.133015 12-13 TACATGG 3415 0.0 24.079712 2 CGCAGAG 6215 0.0 23.924683 8 ACGCAGA 6240 0.0 23.90496 7 TACCGTT 100 0.001652064 23.7527 7 ACATGGG 3365 0.0 23.589964 3 GCAGAGT 6515 0.0 22.604258 9 GTACTTT 3350 0.0 22.404808 14-15 AGAGTAC 5015 0.0 20.508312 10-11 AGTACTT 3405 0.0 20.437859 12-13 TGGACCG 140 4.8249404E-4 20.372637 5 >>END_MODULE