FastQCFastQC Report
Thu 26 May 2016
SRR937318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774880
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.5418903572166013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34290.4425201321494941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22060.2846892422052447No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16660.21500103241792276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.10233842659508569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41800.029.561121
GTATCAA58750.026.9376771
AGGACCG3550.025.424525
GTAAGGT3450.024.7845444
GAGTACT37550.024.15988512-13
GGTGTGC6100.024.1381138
TTAGTCG500.001652842423.75262338-39
TAAGGTG3600.023.7518565
GTACTTT38200.023.4379414-15
GGACCGA3450.023.4046046
GTACATG35400.022.8228261
ATACGTC1252.2537883E-422.8017835
ATCAACG68950.022.5978493
CAACGCA69200.022.5162095
TCAACGC69350.022.4675084
TACATGG35550.022.449012
ACATGGG34750.022.2823163
TATCAAC70050.022.2429922
AACGCAG70450.022.1138486
ACCGAGT3900.021.921968
Produced by FastQC (version 0.11.5)