##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937318_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 774880 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.540486268841626 33.0 31.0 34.0 31.0 34.0 2 32.756367437538714 34.0 31.0 34.0 31.0 34.0 3 32.827933357423085 34.0 31.0 34.0 31.0 34.0 4 36.34457077224861 37.0 37.0 37.0 35.0 37.0 5 36.257284998967584 37.0 37.0 37.0 35.0 37.0 6 36.35011485649391 37.0 37.0 37.0 35.0 37.0 7 36.32330554408424 37.0 37.0 37.0 35.0 37.0 8 36.305824127606854 37.0 37.0 37.0 35.0 37.0 9 38.07434828618625 39.0 38.0 39.0 35.0 39.0 10-11 38.00618224757382 39.0 38.0 39.0 35.0 39.0 12-13 37.94031398410076 39.0 38.0 39.0 35.0 39.0 14-15 39.25388834400165 40.0 39.0 41.0 36.0 41.0 16-17 39.24152449411521 40.0 39.0 41.0 36.0 41.0 18-19 39.254822682221764 40.0 39.0 41.0 36.0 41.0 20-21 39.178065635969446 40.0 39.0 41.0 36.0 41.0 22-23 39.05652939810035 40.0 38.5 41.0 36.0 41.0 24-25 38.91969853396655 40.0 38.0 41.0 35.0 41.0 26-27 38.72094905017551 40.0 38.0 41.0 35.0 41.0 28-29 38.70649648977906 40.0 38.0 41.0 35.0 41.0 30-31 38.823622367334295 40.0 38.0 41.0 35.0 41.0 32-33 38.77494128123064 40.0 38.0 41.0 35.0 41.0 34-35 38.660712626471195 40.0 38.0 41.0 35.0 41.0 36-37 38.51904552963039 40.0 38.0 41.0 35.0 41.0 38-39 38.370750954986576 40.0 38.0 41.0 35.0 41.0 40-41 38.225700108403885 40.0 38.0 41.0 34.0 41.0 42-43 38.01368469956638 40.0 38.0 41.0 34.0 41.0 44-45 37.697762234152385 40.0 38.0 41.0 33.0 41.0 46-47 37.308692958909766 40.0 37.0 41.0 33.0 41.0 48-49 36.9351196314268 40.0 37.0 41.0 32.0 41.0 50-51 36.70199192132976 40.0 37.0 41.0 31.0 41.0 52-53 36.47581173859178 40.0 36.5 41.0 31.0 41.0 54-55 36.2853074024365 39.5 36.0 41.0 31.0 41.0 56-57 36.03925833677472 39.0 35.5 41.0 30.0 41.0 58-59 35.8029004491018 39.0 35.0 40.5 29.5 41.0 60-61 35.46883517447863 38.5 35.0 40.0 29.0 41.0 62-63 35.11754400681396 38.0 35.0 40.0 28.0 41.0 64-65 34.784407908321285 37.5 34.5 40.0 28.0 41.0 66-67 34.40120599318604 37.0 34.0 40.0 27.0 41.0 68-69 33.96650707206277 36.5 34.0 39.0 26.0 41.0 70-71 33.57527423601074 36.0 34.0 39.0 26.0 40.0 72-73 33.14339704212265 35.5 33.0 38.5 25.5 40.0 74-75 32.70692494321702 35.0 33.0 37.5 25.0 39.5 76-77 31.419500438777618 34.0 31.5 36.0 23.0 39.0 78-79 31.984896371051 35.0 32.5 36.0 24.0 39.0 80-81 31.802220343795167 35.0 33.0 36.0 24.0 37.5 82-83 31.4857139169936 35.0 32.0 36.0 22.5 37.0 84-85 31.180299530249847 35.0 32.0 35.0 20.5 37.0 86-87 30.892918903572166 34.5 32.0 35.0 20.0 36.0 88-89 30.575523306834608 34.0 31.5 35.0 18.5 36.0 90-91 30.21499651558951 34.0 31.0 35.0 15.0 35.5 92-93 29.87952844311377 34.0 31.0 35.0 7.5 35.0 94-95 29.560452586206896 34.0 30.5 35.0 2.0 35.0 96-97 29.216764531282262 34.0 30.0 35.0 2.0 35.0 98-99 28.69884304666529 34.0 29.5 35.0 2.0 35.0 100-101 27.225522016312205 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07043189829985153 1101 2 0.13964529365883038 1101 3 0.10300115312547575 1101 4 0.0748697472388713 1101 5 -0.046038052834617815 1101 6 -0.08216528494354947 1101 7 -0.06669977726915022 1101 8 0.08824772614765664 1101 9 0.06852004524363764 1101 10-11 0.02540046670122109 1101 12-13 0.06464010024058098 1101 14-15 0.054564076865375455 1101 16-17 -0.042426780721392277 1101 18-19 0.06872606604760989 1101 20-21 -0.00657780241942163 1101 22-23 -0.09034955602584915 1101 24-25 0.009582751762422959 1101 26-27 0.017403208340489584 1101 28-29 -0.08961054615708264 1101 30-31 -0.07363699017674463 1101 32-33 -0.06248891910760079 1101 34-35 0.030248544671749755 1101 36-37 0.13831491891184555 1101 38-39 0.08583499840535325 1101 40-41 0.11568276586856996 1101 42-43 0.1076058203881729 1101 44-45 0.07601550055014172 1101 46-47 0.09783835203895563 1101 48-49 0.13633533499783823 1101 50-51 0.03585896512195319 1101 52-53 0.2556895083908728 1101 54-55 0.29303713359448835 1101 56-57 0.21386586469547808 1101 58-59 0.20419137223291273 1101 60-61 0.253727614483239 1101 62-63 0.17478796148621 1101 64-65 0.2814948329057003 1101 66-67 0.26321590892280966 1101 68-69 0.0831608842549798 1101 70-71 0.14038449688567312 1101 72-73 0.13632450997725698 1101 74-75 0.14929124404482508 1101 76-77 0.2548810744335164 1101 78-79 0.30662254581964987 1101 80-81 0.17561260532290035 1101 82-83 0.09575934094180383 1101 84-85 0.10830172484499201 1101 86-87 -0.039547844471957916 1101 88-89 0.16233025274739177 1101 90-91 0.19332692365200188 1101 92-93 0.3247054528307629 1101 94-95 -0.23371008975421148 1101 96-97 0.025266133953692815 1101 98-99 -0.1409544509049887 1101 100-101 0.08412599457427561 1102 1 0.1398570528925589 1102 2 0.14450211052809436 1102 3 -0.04332594954662028 1102 4 0.060455650074359824 1102 5 -0.0702897396206339 1102 6 0.02343385863398595 1102 7 -0.013583272825052006 1102 8 0.011764129173307936 1102 9 0.06146723722031311 1102 10-11 0.11961679788880275 1102 12-13 -0.026839341394506278 1102 14-15 0.012104961177918483 1102 16-17 0.012774436300013292 1102 18-19 -0.0785398615790669 1102 20-21 0.07749457091316003 1102 22-23 0.0011474929789798693 1102 24-25 0.06189847303071616 1102 26-27 0.10940594100427603 1102 28-29 -0.09550478925147132 1102 30-31 -0.013078202690657292 1102 32-33 -0.03969371394652654 1102 34-35 0.035899101768933406 1102 36-37 0.03919035733535736 1102 38-39 0.08071759246392674 1102 40-41 0.07300602309111781 1102 42-43 0.19259588983163667 1102 44-45 0.1440912263259193 1102 46-47 0.0837876709858989 1102 48-49 0.11008767677801501 1102 50-51 0.04091791459584471 1102 52-53 0.16271766609656169 1102 54-55 0.02774055505857831 1102 56-57 0.005704496673274662 1102 58-59 -0.1898298692979452 1102 60-61 -0.17433782858860525 1102 62-63 -0.08967402772400135 1102 64-65 0.05248633911053702 1102 66-67 -0.009127843278108116 1102 68-69 -0.07179101241089114 1102 70-71 -0.17036233730924266 1102 72-73 -0.19886487489167592 1102 74-75 -0.4568009058854514 1102 76-77 -0.37039395268183384 1102 78-79 -0.07271626223184313 1102 80-81 -0.021442033866879484 1102 82-83 0.05158315455722828 1102 84-85 -0.003586771203913486 1102 86-87 0.003909961227197556 1102 88-89 0.04680055263611038 1102 90-91 0.010007441449534582 1102 92-93 -0.12393596252004713 1102 94-95 -0.021775110249187435 1102 96-97 0.23394656723584717 1102 98-99 -0.1300871823010432 1102 100-101 -0.00100845530106497 1103 1 0.08940664070037485 1103 2 -0.007015453866422661 1103 3 0.08521802735150885 1103 4 0.12366995285115223 1103 5 -0.0079398106638493 1103 6 -0.006149490181989847 1103 7 0.023306998590101102 1103 8 0.0014606516833026717 1103 9 -0.006491831980909524 1103 10-11 -0.025067612771039194 1103 12-13 0.055367297916539826 1103 14-15 0.11714186295071727 1103 16-17 0.036319996851297276 1103 18-19 0.0735147293341285 1103 20-21 -0.05856733488513299 1103 22-23 -0.008125282873045592 1103 24-25 -0.04804554173352926 1103 26-27 0.0865049007476344 1103 28-29 -0.03696757163275066 1103 30-31 0.12338669417724901 1103 32-33 0.16306697394286118 1103 34-35 0.14061794582597997 1103 36-37 0.2423858863020456 1103 38-39 0.3068199123830979 1103 40-41 0.4113380381880134 1103 42-43 0.5602900731355049 1103 44-45 0.5237818035011443 1103 46-47 0.6669365396913136 1103 48-49 0.6766528657812643 1103 50-51 0.5418937634427863 1103 52-53 0.6182824817469523 1103 54-55 0.6957272275997965 1103 56-57 0.7102111842901735 1103 58-59 0.6368786624343983 1103 60-61 0.5669467706940594 1103 62-63 0.6579297015580181 1103 64-65 0.5912314311128242 1103 66-67 0.5923493777733313 1103 68-69 0.5996819088895151 1103 70-71 0.5501144750004343 1103 72-73 0.4746743156580777 1103 74-75 0.524699547977157 1103 76-77 0.21573023757595777 1103 78-79 0.5677104217046036 1103 80-81 0.40047767226371533 1103 82-83 0.35487131396297755 1103 84-85 0.45033113363180277 1103 86-87 0.5030606276886083 1103 88-89 0.5475207166990401 1103 90-91 0.5064720905951567 1103 92-93 0.41838871645841635 1103 94-95 0.3624903666427457 1103 96-97 0.34761128220482007 1103 98-99 0.03548464035103649 1103 100-101 0.5199360212882915 1104 1 0.043894626937373005 1104 2 0.010946497739837469 1104 3 -0.05459711888040886 1104 4 0.03182614908941872 1104 5 -0.0738605964174468 1104 6 0.0020713644636032313 1104 7 -0.0015951478381026618 1104 8 -0.014923326887050337 1104 9 -0.07228849668472748 1104 10-11 0.08008504649839665 1104 12-13 0.0012933371657339876 1104 14-15 -0.05037633184812762 1104 16-17 -0.14033648996493042 1104 18-19 -0.03380235087693251 1104 20-21 -0.09987799544004616 1104 22-23 -0.1509419454985661 1104 24-25 -0.12809303487319568 1104 26-27 -0.05888895747340683 1104 28-29 -0.12160754361411108 1104 30-31 -0.1065730482667604 1104 32-33 -0.2717776534438272 1104 34-35 -0.19210584731330016 1104 36-37 -0.24596309205662692 1104 38-39 -0.38297608649308046 1104 40-41 -0.33993781074223506 1104 42-43 -0.1844496960185893 1104 44-45 -0.27596908843849377 1104 46-47 -0.5984260862097628 1104 48-49 -0.7184763933026659 1104 50-51 -0.6620231697473713 1104 52-53 -0.7393256575149465 1104 54-55 -0.7130399521005444 1104 56-57 -0.6929748779743363 1104 58-59 -0.7558985508652185 1104 60-61 -0.9133102849622077 1104 62-63 -0.8896625425120774 1104 64-65 -0.8390741183489325 1104 66-67 -0.6793030101617177 1104 68-69 -0.8162424369252008 1104 70-71 -0.6865112413415915 1104 72-73 -0.6456326578281306 1104 74-75 -0.8712639200227059 1104 76-77 -0.6435862499330405 1104 78-79 -0.5503070476115184 1104 80-81 -0.4510533464525821 1104 82-83 -0.4785298529673234 1104 84-85 -0.5259785839879996 1104 86-87 -0.5635995923808998 1104 88-89 -0.43559400963809836 1104 90-91 -0.303691980113431 1104 92-93 -0.256357845677158 1104 94-95 -0.6513178596000984 1104 96-97 -0.5654520253036353 1104 98-99 -0.34135504528594396 1104 100-101 0.11271602490608501 1105 1 0.012627873802919964 1105 2 -0.011107112290424936 1105 3 -0.0034730376006848473 1105 4 0.08746817243572025 1105 5 -0.06728039754153059 1105 6 0.07410768093571818 1105 7 0.08059681065735447 1105 8 0.01881640506279325 1105 9 0.03814384226900813 1105 10-11 -0.041620530674101985 1105 12-13 -0.03373260317148663 1105 14-15 -0.04199760292465271 1105 16-17 0.10497479408176247 1105 18-19 0.07934723840106273 1105 20-21 -0.0798498934080456 1105 22-23 -0.08276425418858224 1105 24-25 -0.09353185891124838 1105 26-27 -0.014403440828360203 1105 28-29 -0.15346443942042498 1105 30-31 -0.12293707766581008 1105 32-33 0.09399839497813645 1105 34-35 0.09077785350814338 1105 36-37 0.19738753483485993 1105 38-39 0.04490761432836621 1105 40-41 0.10085336954310975 1105 42-43 -0.013483418456978313 1105 44-45 -0.07233095614277119 1105 46-47 -0.03214852460146034 1105 48-49 -0.1525360560782758 1105 50-51 0.07228941131617717 1105 52-53 -0.06838748198533295 1105 54-55 0.1902112368315798 1105 56-57 0.008953353759345362 1105 58-59 0.0753768490483182 1105 60-61 0.14914756198980683 1105 62-63 0.2635281189665193 1105 64-65 0.1949768976476136 1105 66-67 0.342984062903561 1105 68-69 0.2687208142637232 1105 70-71 0.11298292208253002 1105 72-73 0.29754935687139294 1105 74-75 0.33339885559338 1105 76-77 0.1522913981430527 1105 78-79 0.34965404188264415 1105 80-81 0.35400718100094153 1105 82-83 0.4083183960599115 1105 84-85 0.37551082370763567 1105 86-87 0.29668575132856745 1105 88-89 0.41126288616821327 1105 90-91 0.3481519455242683 1105 92-93 0.39917176954117295 1105 94-95 0.44011318233678054 1105 96-97 0.44763271313129493 1105 98-99 0.37250574157095073 1105 100-101 0.2661163707772509 1106 1 -0.1746390482915885 1106 2 -0.048259091856202474 1106 3 -0.21675528536569288 1106 4 -0.14394945208535148 1106 5 -0.14904284964880787 1106 6 -0.15248235737846016 1106 7 -0.15536742944481574 1106 8 -0.06373787335355274 1106 9 -0.27651771584082496 1106 10-11 -0.07296791253872925 1106 12-13 -0.2511783247551449 1106 14-15 -0.09478176639846936 1106 16-17 -0.046021374500618606 1106 18-19 -0.14247907066663146 1106 20-21 -0.29370685381673667 1106 22-23 -0.37857523758577116 1106 24-25 -0.08911295725096124 1106 26-27 -0.11937789804419907 1106 28-29 -0.29064834746640145 1106 30-31 -0.3724900789429526 1106 32-33 -0.19688921677154525 1106 34-35 -0.3237623763769122 1106 36-37 -0.33839790577817297 1106 38-39 -0.17299787737022854 1106 40-41 -0.13643909533556098 1106 42-43 -0.27018201360384353 1106 44-45 -0.2766604580584797 1106 46-47 -0.2759863279220198 1106 48-49 -0.42337896795567076 1106 50-51 -0.2082853013274999 1106 52-53 -0.08030319079759352 1106 54-55 -0.16517829062691192 1106 56-57 -0.11535699106823927 1106 58-59 -0.09411868096446341 1106 60-61 -0.032289219619386245 1106 62-63 0.005188399937146926 1106 64-65 -0.2132734216371901 1106 66-67 0.001445110157717977 1106 68-69 -0.04793946670690019 1106 70-71 0.14595865643757122 1106 72-73 0.2818660873950236 1106 74-75 0.006406518428626384 1106 76-77 -0.09494998116729292 1106 78-79 -0.01865374866652303 1106 80-81 0.0892498374504953 1106 82-83 0.1300872338632324 1106 84-85 0.029316698547994235 1106 86-87 0.2637164026695622 1106 88-89 0.1127571390417792 1106 90-91 0.42469728396615736 1106 92-93 0.4087950134747622 1106 94-95 0.23777601758582279 1106 96-97 0.6007425471031986 1106 98-99 0.4715670442529465 1106 100-101 0.7841048765243741 1107 1 -0.07384722198252547 1107 2 0.04824154671340608 1107 3 0.01185263289740135 1107 4 0.06815782760813249 1107 5 -0.0777784818327163 1107 6 0.003992738406985552 1107 7 -0.12400089049206997 1107 8 -0.050302368883563986 1107 9 0.0014388614260951726 1107 10-11 -0.0051262778005423115 1107 12-13 0.03684210947218958 1107 14-15 0.08478400627075189 1107 16-17 0.11083686304727536 1107 18-19 0.04597559089148007 1107 20-21 0.025016006975086214 1107 22-23 0.08114379178843478 1107 24-25 0.03512714492017466 1107 26-27 0.08642031396090744 1107 28-29 -0.04505447773460247 1107 30-31 -0.017343207934011673 1107 32-33 -0.14713187322109178 1107 34-35 -0.017478851472695567 1107 36-37 -0.17242089428391694 1107 38-39 -0.039383573411093664 1107 40-41 0.008669461497127884 1107 42-43 -0.01411560236502396 1107 44-45 0.1804276645468832 1107 46-47 0.1648054983870466 1107 48-49 -0.06276594113575129 1107 50-51 0.1894158480977879 1107 52-53 0.03456270958555052 1107 54-55 -0.009520563934707127 1107 56-57 0.20387672540685742 1107 58-59 0.34309715556172193 1107 60-61 0.3780556079668145 1107 62-63 0.3428384637941093 1107 64-65 0.3072374340460797 1107 66-67 0.23173885217558876 1107 68-69 0.47778211694571837 1107 70-71 0.337120853117014 1107 72-73 0.37854552545376663 1107 74-75 0.590008050995678 1107 76-77 0.4546285628940119 1107 78-79 0.6305161108481627 1107 80-81 0.5299650354070735 1107 82-83 0.6336632236461206 1107 84-85 0.665127681945183 1107 86-87 0.7257087398343138 1107 88-89 0.6340598210341106 1107 90-91 0.6057189953326976 1107 92-93 0.5888460990430886 1107 94-95 0.776626592298463 1107 96-97 1.0174219851619455 1107 98-99 0.9196130212644391 1107 100-101 0.8385110067925723 1108 1 0.08496996693339298 1108 2 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-0.3392823385498147 2316 64-65 -0.411624409541929 2316 66-67 -0.5176368521291153 2316 68-69 -0.5580438916186239 2316 70-71 -0.6983242674599524 2316 72-73 -0.484999662736449 2316 74-75 -0.533823366628841 2316 76-77 -0.701891608392895 2316 78-79 -0.6080910561565673 2316 80-81 -0.6362215771689925 2316 82-83 -0.6835443490381259 2316 84-85 -0.4014172808675269 2316 86-87 -0.5818436604361388 2316 88-89 -0.53109005500826 2316 90-91 -0.7117500152600478 2316 92-93 -0.6519066618313758 2316 94-95 -0.5509325692972098 2316 96-97 -0.6910274175876587 2316 98-99 -0.5577883760761075 2316 100-101 -0.9986875507913808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 2.0 5 11.0 6 21.0 7 52.0 8 90.0 9 247.0 10 408.0 11 816.0 12 1348.0 13 2006.0 14 2570.0 15 3627.0 16 5564.0 17 8889.0 18 8244.0 19 4046.0 20 3068.0 21 2972.0 22 2996.0 23 2960.0 24 3235.0 25 3855.0 26 4491.0 27 5534.0 28 6763.0 29 8588.0 30 10981.0 31 14377.0 32 19327.0 33 26972.0 34 40060.0 35 62975.0 36 110304.0 37 194180.0 38 191029.0 39 22270.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.328156006667264 19.983964489478147 15.521909387745616 21.165970116108973 2 15.621773693991328 21.14443526739624 40.050201321494946 23.18358971711749 3 19.17367850505885 25.480332438571136 30.723595911625022 24.62239314474499 4 12.914257691513525 17.658347098905637 37.808047697708034 31.61934751187281 5 13.320340481552947 38.215892150725686 34.212717076350216 14.251050291371154 6 31.33852983687797 37.29532314680983 17.264866818087963 14.101280198224242 7 27.749716085071235 33.965646293619656 20.823611397893867 17.46102622341524 8 26.27129361965724 35.170219905017554 19.69556576502168 18.86292071030353 9 26.88584038818914 16.65651455709271 20.516854222589302 35.94079083212884 10-11 24.792935680363414 27.329715568862273 27.195243134420817 20.6821056163535 12-13 25.817232180537687 24.945375890021307 27.882752719590215 21.354639209850795 14-15 22.957877348750774 26.010027359074954 26.725686557918642 24.306408734255626 16-17 21.517331715878587 29.33641338013628 27.39256400991121 21.75369089407392 18-19 21.36550175511047 27.630600867231053 30.486526946107784 20.51737043155069 20-21 22.36352725583316 26.482100454263886 30.510917819533347 20.643454470369605 22-23 22.091168488255494 26.342962007967756 30.448731598597444 21.117137905179302 24-25 21.899009843491392 26.382105558620555 30.68091407996748 21.037970517920574 26-27 21.45546080106547 26.716227796218195 30.777951857606716 21.050359545109618 28-29 21.443325179208983 27.083673447000667 30.696051627157555 20.776949746632795 30-31 21.57177406449548 26.73176095272018 30.468165229017284 21.228299753767054 32-33 21.63889892628536 26.746205864133803 30.22035670039232 21.394538509188518 34-35 22.00514918438984 26.747431860417098 30.317468511253352 20.929950443939706 36-37 22.290899611423203 26.46277676901813 30.307828706159285 20.93849491339938 38-39 21.67883202258936 26.98450594803311 30.227984276234455 21.108677753143077 40-41 21.81021577534586 27.033540677266153 30.102661057195952 21.05358249019203 42-43 21.77268793652986 27.190288458994345 29.671607578574566 21.36541602590123 44-45 22.098324901920297 26.995663844724344 29.627039025397483 21.278972227957876 46-47 22.40192029733636 27.592014247367334 28.932608920090853 21.07345653520545 48-49 22.411613676707155 27.595129307201958 28.57941137944674 21.41384563664415 50-51 22.33978164360933 27.936325624612845 28.526287941358664 21.19760479041916 52-53 22.407806633160384 27.989061525050023 28.15089313886869 21.452238702920905 54-55 22.247509291761304 27.9920761924427 28.133969130704113 21.626445385091884 56-57 22.314487404501342 28.113256246128433 28.14545478009498 21.42680156927524 58-59 22.391467065868266 27.60240295271526 28.097834503406975 21.9082954780095 60-61 22.181757175304565 27.882446314268016 28.570810964278344 21.364985546149082 62-63 21.963594362998144 27.712936196572375 28.92918903572166 21.394280404707825 64-65 21.885453231468098 27.880252426182118 28.630045942597565 21.60424839975222 66-67 21.93900991121206 27.76720266363824 28.302640408837497 21.991147016312205 68-69 22.051866095395418 27.737585174478628 28.37342556266777 21.837123167458188 70-71 22.056705554408424 27.78701218253149 28.333096737559366 21.823185525500723 72-73 22.004245818707414 27.523293929382614 28.364520958083833 22.10793929382614 74-75 21.933718769357835 28.158682634730535 27.8404398100351 22.067158785876522 76-77 21.907263060086724 27.710419677885607 28.237791658063184 22.144525603964485 78-79 22.02050640099112 27.51735752632666 28.132807660540987 22.329328412141237 80-81 22.049220524468303 27.959877658476152 28.027178401817054 21.96372341523849 82-83 21.871328048627042 27.98248755448442 27.874212375504513 22.271972021384027 84-85 21.714071856287426 27.906579083212886 28.05182737972331 22.32752168077638 86-87 21.670645777410698 27.860636485649394 27.85799091472228 22.610726822217632 88-89 21.63283347098906 27.539425459425974 27.80591833574231 23.021822733842658 90-91 21.643377356518105 27.29831096036623 27.99924885682979 23.05906282628588 92-93 21.598362030916473 27.219233876483813 28.070288644945812 23.112115447653906 94-95 21.275939500309725 27.542393660953955 27.979751703489576 23.201915135246747 96-97 21.214704212265126 27.51006607474706 27.974331509395 23.300898203592816 98-99 21.087992463227582 27.98162266452006 27.46637328317422 23.464011589078133 100-101 20.747693707588905 28.529296931719543 27.163236134011804 23.559773226679745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 135.0 1 99.0 2 82.0 3 109.0 4 102.5 5 100.5 6 178.0 7 377.5 8 672.5 9 903.5 10 919.0 11 947.0 12 1031.0 13 1125.0 14 1251.0 15 1326.0 16 1407.5 17 1468.5 18 1536.5 19 1622.0 20 1759.5 21 1957.5 22 2216.0 23 2537.5 24 2890.5 25 3584.5 26 4630.5 27 5955.5 28 7216.0 29 8421.0 30 9969.0 31 11777.0 32 13436.5 33 14810.5 34 16347.0 35 17888.0 36 20112.0 37 21801.5 38 22996.0 39 24195.5 40 24696.5 41 25584.5 42 26211.0 43 26962.0 44 28469.5 45 29088.0 46 29005.0 47 30402.5 48 32544.0 49 31136.5 50 28309.5 51 26683.5 52 25550.5 53 25176.0 54 24328.5 55 22299.5 56 19217.0 57 16884.0 58 15508.5 59 13665.5 60 10892.5 61 8511.0 62 6810.5 63 4961.0 64 3431.5 65 2399.5 66 1729.0 67 1341.5 68 967.0 69 671.5 70 447.5 71 292.5 72 228.0 73 197.0 74 150.0 75 90.0 76 68.0 77 45.5 78 29.0 79 25.5 80 16.5 81 11.0 82 5.0 83 3.0 84 3.5 85 1.0 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045039231881065456 2 0.0 3 0.0 4 0.0 5 0.012259962832954779 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005033037373528805 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.03365682428247E-4 24-25 3.2263060086723104E-4 26-27 0.0015486268841627092 28-29 0.0021293619657237247 30-31 5.162089613875697E-4 32-33 0.0 34-35 0.0 36-37 1.2905224034689242E-4 38-39 2.5810448069378485E-4 40-41 0.0 42-43 0.011679227751393764 44-45 0.0 46-47 0.0 48-49 6.452612017344621E-5 50-51 0.0 52-53 6.452612017344621E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.2263060086723104E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007872186661160437 92-93 0.0023229403262440638 94-95 0.0 96-97 0.0 98-99 0.0016131530043361553 100-101 0.01258259343382201 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 774880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.02813930532172 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06174436694431 43.17626148502248 2 12.72968690623984 14.264413425918713 3 4.1944529424570645 7.050221813088029 4 1.9201908717253944 4.303388866153502 5 1.0797613942370168 3.0248510906409987 6 0.6498185981046023 2.184487616267612 7 0.4355879910050227 1.7083629247828247 8 0.3034695248991826 1.3602266252776962 9 0.24471078862650375 1.2339621139212802 >10 1.2515404916632353 12.6556355361393 >50 0.0877263991160142 3.369466689964705 >100 0.039685185236317144 3.8987740814657883 >500 6.962313194980031E-4 0.27462015931723704 >1k 9.283084259973375E-4 1.4953275720398795 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4199 0.5418903572166013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3429 0.4425201321494941 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2206 0.2846892422052447 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1666 0.21500103241792276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 793 0.10233842659508569 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.452612017344621E-5 0.0 20-21 0.0 0.0 0.0 1.2905224034689242E-4 0.0 22-23 0.0 0.0 0.0 3.2263060086723104E-4 0.0 24-25 0.0 0.0 0.0 5.807350815610159E-4 0.0 26-27 0.0 0.0 0.0 7.743134420813546E-4 0.0 28-29 0.0 0.0 0.0 0.0014841007639892628 0.0 30-31 0.0 0.0 0.0 0.0038070410902333266 0.0 32-33 0.0 0.0 0.0 0.005807350815610159 0.0 34-35 0.0 0.0 0.0 0.008194817262027669 0.0 36-37 1.2905224034689242E-4 0.0 0.0 0.011033966549659302 0.0 38-39 1.2905224034689242E-4 0.0 0.0 0.013744063596944044 0.0 40-41 1.2905224034689242E-4 0.0 0.0 0.021551724137931036 0.0 42-43 1.2905224034689242E-4 0.0 0.0 0.03161779888498864 0.0 44-45 1.2905224034689242E-4 0.0 0.0 0.05065300433615527 0.0 46-47 1.9357836052033865E-4 0.0 0.0 0.09859591162502582 0.0 48-49 2.5810448069378485E-4 0.0 0.0 0.13731158372909352 0.0 50-51 2.5810448069378485E-4 0.0 0.0 0.1849963865372703 0.0 52-53 2.5810448069378485E-4 0.0 0.0 0.2443604170968408 0.0 54-55 2.5810448069378485E-4 0.0 0.0 0.3175975634937023 0.0 56-57 2.5810448069378485E-4 0.0 0.0 0.40735339665496595 0.0 58-59 2.5810448069378485E-4 0.0 0.0 0.5419548833367748 0.0 60-61 2.5810448069378485E-4 0.0 0.0 0.7143686764402231 0.0 62-63 2.5810448069378485E-4 0.0 0.0 0.937370947759653 0.0 64-65 2.5810448069378485E-4 0.0 0.0 1.1229480693784843 0.0 66-67 2.5810448069378485E-4 0.0 0.0 1.3953773487507743 0.0 68-69 2.5810448069378485E-4 0.0 0.0 1.7063932479867852 0.0 70-71 3.2263060086723104E-4 0.0 0.0 1.9768867437538715 0.0 72-73 3.8715672104067724E-4 0.0 0.0 2.4577999174065663 0.0 74-75 3.8715672104067724E-4 0.0 0.0 2.8638627916580632 0.0 76-77 3.8715672104067724E-4 0.0 0.0 3.2275965310757795 0.0 78-79 3.8715672104067724E-4 0.0 0.0 3.709542122651249 0.0 80-81 3.8715672104067724E-4 0.0 0.0 4.078502477803015 0.0 82-83 3.8715672104067724E-4 0.0 0.0 4.507665703076606 0.0 84-85 4.5168284121412344E-4 0.0 0.0 5.263718253148875 0.0 86-87 5.162089613875697E-4 0.0 0.0 6.101009188519512 0.0 88-89 5.162089613875697E-4 0.0 0.0 7.156850092917613 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4180 0.0 29.56112 1 GTATCAA 5875 0.0 26.937677 1 AGGACCG 355 0.0 25.42452 5 GTAAGGT 345 0.0 24.784544 4 GAGTACT 3755 0.0 24.159885 12-13 GGTGTGC 610 0.0 24.138113 8 TTAGTCG 50 0.0016528424 23.752623 38-39 TAAGGTG 360 0.0 23.751856 5 GTACTTT 3820 0.0 23.43794 14-15 GGACCGA 345 0.0 23.404604 6 GTACATG 3540 0.0 22.822826 1 ATACGTC 125 2.2537883E-4 22.801783 5 ATCAACG 6895 0.0 22.597849 3 CAACGCA 6920 0.0 22.516209 5 TCAACGC 6935 0.0 22.467508 4 TACATGG 3555 0.0 22.44901 2 ACATGGG 3475 0.0 22.282316 3 TATCAAC 7005 0.0 22.242992 2 AACGCAG 7045 0.0 22.113848 6 ACCGAGT 390 0.0 21.92196 8 >>END_MODULE