FastQCFastQC Report
Thu 26 May 2016
SRR937317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870075
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.4013447116627876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.306755164784645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.20205154728040686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12740.14642415883688187No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11290.1297589288279746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.034.207071
GTATCAA50250.032.1726261
ACGCGTG650.005844974429.2290489
TGGACCG908.918792E-426.4025445
ATCAACG60400.026.2059973
CAACGCA61150.025.8801085
TCAACGC61350.025.80024
TATCAAC61700.025.7308852
AACGCAG62750.025.2828396
TAGAGTG2052.6528141E-823.1827245
GAGTACT36300.022.76725612-13
ACGCAGA69100.022.6844887
CGCAGAG69450.022.5701668
GTACATG37400.022.5032541
TACATGG36450.022.4297452
ACCGTGG1503.0321373E-522.1666418
GTACTTT37650.021.95090114-15
ACATGGG36450.021.7777183
AGTACTT38700.021.41669812-13
GCAGAGT72300.021.350759
Produced by FastQC (version 0.11.5)