##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937317_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 870075 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04827629802028 33.0 31.0 34.0 31.0 34.0 2 32.16116426744821 33.0 31.0 34.0 31.0 34.0 3 32.2119667844726 34.0 31.0 34.0 30.0 34.0 4 35.80829698589202 37.0 35.0 37.0 35.0 37.0 5 35.797292187455106 37.0 35.0 37.0 35.0 37.0 6 35.858879981610784 37.0 36.0 37.0 35.0 37.0 7 35.8176134241301 37.0 35.0 37.0 35.0 37.0 8 35.81527569462403 37.0 35.0 37.0 35.0 37.0 9 37.48829698589202 39.0 37.0 39.0 35.0 39.0 10-11 37.40530816308939 39.0 37.0 39.0 35.0 39.0 12-13 37.32125563888171 39.0 37.0 39.0 35.0 39.0 14-15 38.56707008016551 40.0 38.0 41.0 34.5 41.0 16-17 38.52334453926386 40.0 38.0 41.0 34.0 41.0 18-19 38.48251989770996 40.0 38.0 41.0 34.0 41.0 20-21 38.41744619716691 40.0 38.0 41.0 34.0 41.0 22-23 38.293301152199525 40.0 38.0 41.0 34.0 41.0 24-25 38.158498405309885 40.0 38.0 41.0 34.0 41.0 26-27 37.99594862511853 40.0 38.0 41.0 33.5 41.0 28-29 37.8260598224291 40.0 38.0 41.0 33.0 41.0 30-31 37.80354911932879 40.0 37.5 41.0 33.0 41.0 32-33 37.654881475734854 40.0 37.0 41.0 33.0 41.0 34-35 37.46346694250496 40.0 37.0 41.0 33.0 41.0 36-37 37.52506680458581 40.0 37.0 41.0 33.0 41.0 38-39 37.50651380628106 40.0 37.0 41.0 33.0 41.0 40-41 37.362113036232515 40.0 37.0 41.0 32.5 41.0 42-43 37.1380105163348 40.0 37.0 41.0 32.0 41.0 44-45 36.90538286929288 40.0 37.0 41.0 31.0 41.0 46-47 36.56580869465276 40.0 36.5 41.0 30.5 41.0 48-49 36.261463666925266 39.5 36.0 41.0 30.0 41.0 50-51 35.34987845875355 38.5 35.0 40.0 28.0 40.5 52-53 35.46154584374911 38.5 35.0 40.0 28.5 40.5 54-55 35.81806970663449 39.0 35.5 40.5 28.5 41.0 56-57 35.61145475964716 39.0 35.0 41.0 28.0 41.0 58-59 35.389227365457 39.0 35.0 40.5 27.5 41.0 60-61 35.111219722437724 38.5 35.0 40.0 27.0 41.0 62-63 34.80549952590294 38.0 34.5 40.0 26.0 41.0 64-65 34.457483550268655 38.0 34.0 40.0 26.0 41.0 66-67 34.0692118495532 37.0 34.0 40.0 25.5 41.0 68-69 33.59718874809643 36.5 33.5 39.0 24.0 41.0 70-71 33.161599862080855 36.0 33.0 39.0 23.0 40.5 72-73 32.7658908714766 35.5 33.0 38.5 22.0 40.0 74-75 32.31602045800649 35.0 32.0 37.5 20.5 39.5 76-77 31.87811050771485 35.0 32.0 37.0 19.5 39.0 78-79 31.421457345631122 35.0 31.5 36.5 18.0 39.0 80-81 30.993979829325056 35.0 31.0 36.0 16.0 38.5 82-83 30.579174209119905 34.5 31.0 35.5 10.0 37.0 84-85 30.14021147602218 34.0 30.5 35.0 7.0 37.0 86-87 29.74681550441054 34.0 30.0 35.0 3.5 36.0 88-89 29.40399103525558 34.0 30.0 35.0 2.0 36.0 90-91 29.02216647990116 34.0 29.0 35.0 2.0 35.5 92-93 28.627638996638222 34.0 29.0 35.0 2.0 35.0 94-95 28.154224635807257 34.0 29.0 35.0 2.0 35.0 96-97 27.596437088756716 34.0 27.5 35.0 2.0 35.0 98-99 26.961973393098297 33.0 26.5 35.0 2.0 35.0 100-101 25.189618136367553 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10626609884564076 1101 2 -0.2677564089056794 1101 3 -0.06065894995885657 1101 4 -0.1176991374628571 1101 5 -0.10188688813414615 1101 6 -0.32020486866502296 1101 7 -0.1263951501652869 1101 8 -0.19484115823775738 1101 9 -0.1432815349333012 1101 10-11 -0.18790758707369548 1101 12-13 -0.13665326022464086 1101 14-15 -0.13480843676042298 1101 16-17 -0.1307698496697114 1101 18-19 -0.032390091374843166 1101 20-21 -0.11164168992084456 1101 22-23 -0.10493599825927191 1101 24-25 -0.03160460422088818 1101 26-27 -0.018548614701487054 1101 28-29 -0.09406735589956128 1101 30-31 -0.08139774798323884 1101 32-33 0.06564292569823493 1101 34-35 -0.05071814330824509 1101 36-37 -0.0208072406004689 1101 38-39 0.13770619235885562 1101 40-41 0.12799697693072432 1101 42-43 0.06760917244314868 1101 44-45 0.12772053909669268 1101 46-47 0.37927808333321167 1101 48-49 0.3722992441348367 1101 50-51 0.3420675540516456 1101 52-53 0.15063769379131742 1101 54-55 0.3368722644408706 1101 56-57 0.38892006187655426 1101 58-59 0.22752885011174584 1101 60-61 0.14574828093824266 1101 62-63 0.15678777239237007 1101 64-65 0.07969778896670476 1101 66-67 0.08316965203152193 1101 68-69 0.11777715253731458 1101 70-71 0.09828377526292087 1101 72-73 0.05689409334571849 1101 74-75 0.12666709222362016 1101 76-77 0.12548581559675398 1101 78-79 0.10428361401972452 1101 80-81 0.020159896698064728 1101 82-83 0.13060081447741823 1101 84-85 0.07218480721644482 1101 86-87 0.20961713582848063 1101 88-89 0.08875976832558408 1101 90-91 0.12252961622658987 1101 92-93 0.1909356489433094 1101 94-95 0.16753426119832326 1101 96-97 0.1484008475770331 1101 98-99 0.12197718620658904 1101 100-101 0.4446232344506278 1102 1 -0.10945298288358885 1102 2 -0.18834112248163493 1102 3 -0.1580956809375138 1102 4 -0.1391041404698612 1102 5 -0.1665673057557413 1102 6 -0.1589529791277826 1102 7 -0.08073959587393631 1102 8 -0.06483882848574041 1102 9 0.014162022760565662 1102 10-11 -0.2219953807985391 1102 12-13 -0.07056713525241065 1102 14-15 0.17646864660677863 1102 16-17 0.10582161476906293 1102 18-19 -0.0674184547511345 1102 20-21 0.014685049672479522 1102 22-23 0.05808650281079508 1102 24-25 0.22894212897916333 1102 26-27 0.1261736842761323 1102 28-29 0.04162498680154414 1102 30-31 0.04169063630664738 1102 32-33 -0.12271400314680392 1102 34-35 -0.007234405519120912 1102 36-37 0.07090100943068478 1102 38-39 0.34384454712049006 1102 40-41 0.2243874867129776 1102 42-43 0.053524196092844534 1102 44-45 -0.07319158590804875 1102 46-47 0.26877848697629503 1102 48-49 0.3279743575070668 1102 50-51 0.4178018322890509 1102 52-53 0.4942498388427552 1102 54-55 0.6599519798860385 1102 56-57 0.630712562242266 1102 58-59 0.42828276870044846 1102 60-61 0.5106695299020458 1102 62-63 0.6577581411975331 1102 64-65 0.6456140587628241 1102 66-67 0.6153998748750098 1102 68-69 0.6384485545604122 1102 70-71 0.4658284333582685 1102 72-73 0.5261885035662885 1102 74-75 0.3164985373742226 1102 76-77 0.29369689112948194 1102 78-79 0.18069026952525746 1102 80-81 0.22113839254514644 1102 82-83 0.269573773099939 1102 84-85 0.27478383522223027 1102 86-87 0.2808679959404152 1102 88-89 0.412551412642884 1102 90-91 0.22541038168558103 1102 92-93 0.024533111139017905 1102 94-95 -0.15520601479602192 1102 96-97 0.0895030911824044 1102 98-99 0.09187760285293578 1102 100-101 0.49714099203722384 1103 1 -0.082795991559383 1103 2 -0.11710808754808255 1103 3 -0.0721067025419444 1103 4 -0.03486027664361302 1103 5 -0.11530786868741671 1103 6 -0.2089249511839526 1103 7 0.011228467963235289 1103 8 0.05733224805722159 1103 9 -0.06913338549013304 1103 10-11 -0.0010199957987921948 1103 12-13 0.032760249335545666 1103 14-15 0.038675365403953776 1103 16-17 0.04098635818940011 1103 18-19 0.0039802997159128495 1103 20-21 -0.005783195891233106 1103 22-23 -0.028473705794944237 1103 24-25 -0.08461806016708806 1103 26-27 -0.15650994269672935 1103 28-29 -0.24799312383256478 1103 30-31 -0.2296060499931798 1103 32-33 -0.1579626734672459 1103 34-35 0.08874670747585611 1103 36-37 0.1627507470100369 1103 38-39 -0.05396236828031675 1103 40-41 -0.06790912865387355 1103 42-43 -0.21441761124187764 1103 44-45 -0.26057267115756844 1103 46-47 -0.12935202092268838 1103 48-49 0.01373528322643125 1103 50-51 0.17976926212346456 1103 52-53 0.12649653747739364 1103 54-55 0.2837623615870868 1103 56-57 0.3869165303462836 1103 58-59 0.21821254504813226 1103 60-61 0.4405375255054551 1103 62-63 0.21987628792585667 1103 64-65 0.36512119380478936 1103 66-67 0.49579399861101336 1103 68-69 0.18393448629024078 1103 70-71 0.17753953148243085 1103 72-73 0.37074923067329024 1103 74-75 0.40486251522052896 1103 76-77 0.28187054260246924 1103 78-79 0.47015772603800343 1103 80-81 0.4520538109273353 1103 82-83 0.5108306553254529 1103 84-85 0.7227905870377711 1103 86-87 0.6458228970014623 1103 88-89 0.5319592415098455 1103 90-91 0.6136617455761737 1103 92-93 0.47963096412947337 1103 94-95 0.5838466093162964 1103 96-97 0.562629534558738 1103 98-99 0.8254985181646042 1103 100-101 0.9732802039780672 1104 1 -0.15336709796558523 1104 2 -0.13991305848895053 1104 3 -0.012852613571219251 1104 4 -0.18023990466273432 1104 5 -0.07877253290300246 1104 6 -0.09573678355864246 1104 7 -0.13034633306118337 1104 8 -0.09704776381833824 1104 9 -0.08681702181014828 1104 10-11 -0.07854924985776535 1104 12-13 0.04688196781284404 1104 14-15 0.06173411643106874 1104 16-17 0.17543582408915626 1104 18-19 0.08463700910660776 1104 20-21 -0.0778728593570932 1104 22-23 0.12745710108328012 1104 24-25 0.029011692305488168 1104 26-27 0.23049044415859044 1104 28-29 0.17307039841011118 1104 30-31 -0.024972707863135213 1104 32-33 -0.09843793571916848 1104 34-35 -0.03332413802792189 1104 36-37 -0.014271546652139477 1104 38-39 0.13180465485303472 1104 40-41 -0.044430159788227286 1104 42-43 0.09336627758370497 1104 44-45 -0.03799554000461569 1104 46-47 0.011828427589570367 1104 48-49 -0.03680931309454394 1104 50-51 -0.1427398694613231 1104 52-53 -0.24709099078703645 1104 54-55 -0.3829535366669674 1104 56-57 -0.2506648892028096 1104 58-59 -0.3217133976492974 1104 60-61 -0.22042464964103914 1104 62-63 -0.060398514298107386 1104 64-65 -0.06395870531294179 1104 66-67 0.0476847164825287 1104 68-69 -0.10102493888474129 1104 70-71 -0.22727531776394727 1104 72-73 -0.049205456273796244 1104 74-75 -0.012193755734138279 1104 76-77 -0.19049104416408014 1104 78-79 -0.11749836558373161 1104 80-81 -0.18389546904195697 1104 82-83 -0.08769524598745804 1104 84-85 0.019391283377338198 1104 86-87 0.16294386875802402 1104 88-89 0.09856395634649928 1104 90-91 0.28068952718585294 1104 92-93 0.2005677087869735 1104 94-95 0.12783865629073077 1104 96-97 0.050515565183285815 1104 98-99 0.051502992491204935 1104 100-101 -0.006267820060937623 1105 1 0.19087169244108537 1105 2 0.06330271687229327 1105 3 0.10210157978495715 1105 4 0.185291526887994 1105 5 0.2301709349951082 1105 6 0.1172734205229915 1105 7 -0.0128783604706868 1105 8 0.1153014094276017 1105 9 0.16553848243534475 1105 10-11 0.20215609797479317 1105 12-13 0.2995866080631018 1105 14-15 0.2479586062269874 1105 16-17 0.23092051796670177 1105 18-19 0.08207202325825591 1105 20-21 0.11474780204615342 1105 22-23 0.12801106354417868 1105 24-25 0.23016989281196487 1105 26-27 0.16901923158596333 1105 28-29 0.19383700797364867 1105 30-31 -0.06185078025998081 1105 32-33 -0.09252124422505403 1105 34-35 0.12777186971824506 1105 36-37 0.12460544575066024 1105 38-39 0.06113487234149062 1105 40-41 0.13604987894259324 1105 42-43 0.06348954586911049 1105 44-45 0.002029411581851548 1105 46-47 0.036240017042871386 1105 48-49 0.04051732924121865 1105 50-51 0.12567589100344634 1105 52-53 0.13394207937533764 1105 54-55 0.08146717687392879 1105 56-57 0.12614596287525615 1105 58-59 0.2240885910293855 1105 60-61 0.39999003082969153 1105 62-63 0.4495634997045741 1105 64-65 0.3942566976365569 1105 66-67 0.3808628782581778 1105 68-69 0.2905753143552374 1105 70-71 0.6560806706168094 1105 72-73 0.47753836169135155 1105 74-75 0.3086715429400826 1105 76-77 0.30891775220118234 1105 78-79 0.4617322317646959 1105 80-81 0.37684954064074816 1105 82-83 0.43619496703205485 1105 84-85 0.39088595788948055 1105 86-87 0.5792862268691543 1105 88-89 0.623948471466413 1105 90-91 0.632721033218921 1105 92-93 0.5309993339917973 1105 94-95 0.36971077009975417 1105 96-97 0.21487483078733405 1105 98-99 0.2993662801420349 1105 100-101 0.573675014737212 1106 1 0.16754976707125024 1106 2 0.13376726281431672 1106 3 -0.16045835145183318 1106 4 0.14677190729363332 1106 5 -0.06067102002491964 1106 6 -0.02193461972556321 1106 7 0.058942523988200435 1106 8 0.012468081351393323 1106 9 0.13647774685827585 1106 10-11 -0.0025090756680796744 1106 12-13 -0.1036274016520693 1106 14-15 0.10433458010958674 1106 16-17 -0.007523990439437966 1106 18-19 0.2713472890059663 1106 20-21 0.04666670561994124 1106 22-23 0.1686036336479546 1106 24-25 -0.056640503680476684 1106 26-27 0.09910569816641868 1106 28-29 0.21137182329081128 1106 30-31 0.22525927241643018 1106 32-33 0.28122000266009906 1106 34-35 0.2743254293182815 1106 36-37 -0.19413312000020966 1106 38-39 -0.19841962565553928 1106 40-41 0.07244982531990729 1106 42-43 0.24495305090018604 1106 44-45 0.620338404421652 1106 46-47 0.4082947121821334 1106 48-49 0.2914350263045904 1106 50-51 0.320649552802017 1106 52-53 0.5077079690387762 1106 54-55 0.6367231857689859 1106 56-57 0.3213709810123362 1106 58-59 0.40950184056413264 1106 60-61 0.46403069732812696 1106 62-63 0.5429596294083794 1106 64-65 0.4533185372101016 1106 66-67 0.4261996760375766 1106 68-69 0.41831756272289766 1106 70-71 0.5481127732569391 1106 72-73 0.7171657234552242 1106 74-75 0.7547048442049444 1106 76-77 0.5655545452491566 1106 78-79 0.4811172268755719 1106 80-81 0.4945227498007654 1106 82-83 0.54075131816602 1106 84-85 0.48104402652651856 1106 86-87 0.6659962779684179 1106 88-89 0.7126890874964396 1106 90-91 0.6761573799368996 1106 92-93 0.8236293690042551 1106 94-95 0.8803544788970292 1106 96-97 0.794113191510295 1106 98-99 0.7830714894282202 1106 100-101 0.817708505258615 1107 1 -0.06697420182213065 1107 2 0.023392303888201127 1107 3 0.009503556323608109 1107 4 0.052865446219506396 1107 5 0.10703699638029462 1107 6 0.25347581486655457 1107 7 0.06095058055453961 1107 8 0.22468193329068953 1107 9 0.24244471737026885 1107 10-11 0.13709101682422187 1107 12-13 0.35256045508221945 1107 14-15 0.4685254550137472 1107 16-17 0.45830983983719165 1107 18-19 0.4081275832775333 1107 20-21 0.48066857980607836 1107 22-23 0.3511761366600368 1107 24-25 0.42294668856964535 1107 26-27 0.3021835012308287 1107 28-29 0.2291023542696493 1107 30-31 0.2738152971946235 1107 32-33 -0.022560011700541338 1107 34-35 0.12873402623439034 1107 36-37 0.28044366686587807 1107 38-39 0.07959763146089216 1107 40-41 0.24834097648396636 1107 42-43 0.3414322583871936 1107 44-45 0.2891015632771783 1107 46-47 0.27491107601763787 1107 48-49 0.02472008514673263 1107 50-51 0.26940881724481613 1107 52-53 0.2917919909935236 1107 54-55 0.2310142201006684 1107 56-57 0.47563475445487313 1107 58-59 0.33034059970687224 1107 60-61 0.32144772808181443 1107 62-63 0.4925391545334321 1107 64-65 0.4256463007218869 1107 66-67 0.34441944261620705 1107 68-69 0.43576453177448826 1107 70-71 0.45010987678746517 1107 72-73 0.4184119355303295 1107 74-75 0.4957908985563435 1107 76-77 0.37687633327140446 1107 78-79 0.4814433839421035 1107 80-81 0.6844252677820002 1107 82-83 0.51221135784035 1107 84-85 0.50843878610738 1107 86-87 0.7474235802356972 1107 88-89 0.9172625884517878 1107 90-91 0.7105231383129258 1107 92-93 0.9600287410302037 1107 94-95 0.6377386505432732 1107 96-97 0.7361095648384364 1107 98-99 0.8320765886748944 1107 100-101 1.1921940168262388 1108 1 -0.0033900317502144617 1108 2 0.017869402701549575 1108 3 6.536062293420741E-4 1108 4 -0.0639498001953882 1108 5 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-1.429043115355249 2316 64-65 -1.048235385403558 2316 66-67 -1.4502859605876992 2316 68-69 -1.1291994921190778 2316 70-71 -1.1685586678772104 2316 72-73 -1.1749290908068915 2316 74-75 -1.0246764514300821 2316 76-77 -1.2253670824223235 2316 78-79 -1.1618603086997616 2316 80-81 -1.1388964129130876 2316 82-83 -1.1397418732212863 2316 84-85 -1.2979775185355749 2316 86-87 -1.6011984670083983 2316 88-89 -1.3238655679424518 2316 90-91 -1.5619042306797404 2316 92-93 -1.2785098144951839 2316 94-95 -1.7215981672682936 2316 96-97 -1.9199334028509476 2316 98-99 -2.569454092834743 2316 100-101 -3.1159141830108545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2834.0 3 428.0 4 344.0 5 419.0 6 531.0 7 521.0 8 765.0 9 986.0 10 1196.0 11 1547.0 12 2273.0 13 3093.0 14 3556.0 15 4651.0 16 6274.0 17 8813.0 18 7234.0 19 4471.0 20 4400.0 21 4482.0 22 4742.0 23 5184.0 24 5769.0 25 6383.0 26 7573.0 27 8933.0 28 10595.0 29 12931.0 30 16374.0 31 20876.0 32 27408.0 33 36999.0 34 52448.0 35 79019.0 36 125085.0 37 192500.0 38 173591.0 39 24845.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97271345030588 19.240527632948588 15.30875611097214 22.478002805773393 2 14.761526662663973 20.152832213001687 41.26976810114446 23.815873023189884 3 18.358456645000693 25.143594707970312 30.674411100354952 25.823537546674043 4 12.03370238777107 16.660693109357588 38.52575678824681 32.77984771462454 5 12.924027148799851 37.66100874615411 35.14064138463488 14.274322720411151 6 30.703945693873862 36.855998700854464 18.109259521596567 14.330796083675107 7 27.25139799932044 33.1979198461193 21.79312489561797 17.7575572589423 8 25.60212656175137 34.7106153023993 20.60083661961449 19.086421516234843 9 25.955384486037065 16.639773893394054 21.09395425118887 36.31088736938001 10-11 24.13162958359802 26.805837840452863 28.219050904581916 20.843481671367204 12-13 25.13856509679976 24.433011896095756 28.550284271536906 21.878138735567575 14-15 22.344030629843136 25.60429619366961 27.070603769304164 24.98106940718309 16-17 21.14409151534278 28.961353380341066 27.753948856634103 22.140606247682054 18-19 21.112111124738934 27.510490066179404 30.30881994745034 21.06857886163132 20-21 22.048692789945186 26.407767996632426 30.225197963100637 21.318341250321758 22-23 21.6892848649156 26.274501448535947 30.30892310810725 21.727290578441202 24-25 21.45506639535031 26.419487792491353 30.431250208727036 21.6941956034313 26-27 21.18545391724972 26.654919236417037 30.40977800811955 21.7498488382137 28-29 21.238329583559114 26.85579272453585 30.435075543506112 21.47080214839892 30-31 21.285769405748447 26.537918881408835 30.252235857803473 21.924075855039245 32-33 21.090443145498 26.61418411126148 30.197068732183478 22.098304011057042 34-35 21.60462937422944 26.72192140452146 29.926296944021036 21.747152277228064 36-37 21.66185366359268 26.390247694509146 30.17773081540814 21.770167826490038 38-39 21.297874398583087 26.854402406358606 29.92476674581916 21.922956449239145 40-41 21.49510162388625 26.78318279862129 30.00348938042193 21.718226197070535 42-43 21.279360739953407 26.760550404834753 29.69406215488859 22.266026700323245 44-45 21.499452351089595 26.798517028944197 29.630224691537183 22.071805928429026 46-47 21.73046825966237 27.10340268764762 29.146374186237466 22.019754866452544 48-49 21.568520184226923 27.174968356872288 29.01666173352513 22.239849725375656 50-51 21.576643529569626 27.41512766483094 28.922273520683735 22.0859552849157 52-53 21.817039409386172 27.4686226324795 28.489492116567796 22.22484584156654 54-55 21.68170592923983 27.28451274632216 28.649104094580334 22.384677229857676 56-57 21.723412071012458 27.45359052338121 28.478796330309038 22.344201075297295 58-59 21.841916643398513 27.14365902031958 28.56991528473024 22.444509051551673 60-61 21.634045444208066 27.16761042228626 29.09246885894332 22.105875274562354 62-63 21.373981193388435 27.166640949404545 29.246745230983233 22.212632626223776 64-65 21.40847405810694 27.216001372559518 28.994398644482327 22.381125924851215 66-67 21.372508261410076 27.103183126051928 28.71913163496746 22.805176977570536 68-69 21.426729031797464 27.050933058816213 28.990573837505213 22.53176407188111 70-71 21.46320061171095 26.990363710063754 28.96788747835059 22.57854819987471 72-73 21.391034249241606 26.79538444883114 28.9658774453461 22.847703856581155 74-75 21.256199146557528 27.4487528580995 28.615312331040325 22.679735664302648 76-77 21.339972233626796 26.900214380355553 28.762784077004177 22.997029309013474 78-79 21.316523626773844 26.98430064000875 28.75893579471352 22.940239938503883 80-81 21.437972619586358 27.158288914145167 28.64027193870325 22.763466527565228 82-83 21.352714198270554 27.076280629058513 28.724130167272534 22.846875005398395 84-85 21.29162595779788 26.909996793187048 28.60638357282718 23.191993676187888 86-87 21.295288191431723 26.985592875373737 28.797552349110372 22.921566584084164 88-89 21.27961315761593 26.541692193596234 28.610755397506114 23.567939251281718 90-91 21.18938196172541 26.30010743785474 28.964174714909678 23.54633588551017 92-93 21.13528266903136 26.37914157060104 29.072011792751606 23.413563967616 94-95 20.91041420990566 26.26884028596698 29.117569833431606 23.703175670695757 96-97 20.69297079937264 26.407521366733995 29.261773870747092 23.63773396314627 98-99 20.65619758477555 26.839013250741477 28.811567466041126 23.693221698441842 100-101 20.569674729017613 26.615775847030466 28.94276711491196 23.87178230903996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1122.0 1 644.5 2 158.5 3 146.0 4 135.0 5 140.0 6 185.5 7 355.0 8 632.0 9 789.0 10 821.0 11 858.0 12 901.0 13 970.5 14 1085.0 15 1173.0 16 1213.0 17 1279.5 18 1402.0 19 1580.0 20 1715.5 21 1877.0 22 2213.5 23 2620.5 24 3108.5 25 3925.0 26 5186.0 27 6726.5 28 7943.0 29 9149.5 30 10891.5 31 13050.5 32 15068.5 33 16787.5 34 18621.5 35 20336.5 36 22606.5 37 25177.0 38 26376.0 39 26981.5 40 27931.5 41 29173.0 42 29993.0 43 30936.5 44 32698.0 45 33640.5 46 33855.0 47 34744.5 48 36538.0 49 35581.5 50 32384.5 51 30294.0 52 28718.5 53 27798.5 54 26762.5 55 24060.5 56 20915.0 57 18538.0 58 16940.0 59 14935.5 60 12092.0 61 9696.5 62 7525.0 63 5397.5 64 3789.5 65 2766.5 66 1964.5 67 1438.5 68 1050.5 69 725.5 70 518.0 71 343.0 72 245.0 73 198.0 74 150.0 75 103.5 76 70.0 77 44.0 78 30.0 79 23.5 80 18.5 81 11.5 82 7.0 83 7.0 84 4.5 85 3.0 86 3.5 87 3.0 88 4.5 89 2.5 90 1.0 91 2.0 92 1.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2962962962962963 2 0.26733327586702293 3 0.2706663218688044 4 0.271470850214062 5 0.26032238600120683 6 0.20825790880096545 7 0.21492400080452834 8 0.20906243714622305 9 0.20825790880096545 10-11 0.21969370456569837 12-13 0.21733758584030113 14-15 0.2036031376605465 16-17 0.21251041576875557 18-19 0.201534350487027 20-21 0.20624658793782147 22-23 0.20509726173031062 24-25 0.1972243772088613 26-27 0.2068787173519524 28-29 0.20693618366232797 30-31 0.2114760221819958 32-33 0.21262534838950667 34-35 0.20613165531707037 36-37 0.20314340717754215 38-39 0.1964198488636037 40-41 0.19837370341637214 42-43 0.19492572479383963 44-45 0.20894750452547195 46-47 0.19906329914087867 48-49 0.20664885211045025 50-51 0.21199321897537568 52-53 0.21762491739217882 54-55 0.21871677728931413 56-57 0.19124788092980488 58-59 0.1813062092348361 60-61 0.189351492687412 62-63 0.1904433525845473 64-65 0.18670804241013705 66-67 0.198890900209752 68-69 0.18860443065252996 70-71 0.1943510616900842 72-73 0.2060741890066948 74-75 0.19647731517397926 76-77 0.20210901359078243 78-79 0.19854610234749875 80-81 0.18894922851478319 82-83 0.20245381145303568 84-85 0.18521391834037296 86-87 0.18969629054966525 88-89 0.19664971410510587 90-91 0.19147774617130706 92-93 0.2005574232106428 94-95 0.1980289055541189 96-97 0.19182254403356033 98-99 0.19636238255322816 100-101 0.1855587162026262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 870075.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.99312772509882 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12567726246313 45.307523800918744 2 12.315948525063709 14.284807517395242 3 3.9893396957860885 6.940628595495886 4 1.7827016302347058 4.135377733517728 5 0.9784721510779267 2.83723302164572 6 0.5889106424912067 2.049166206519754 7 0.3929531291264731 1.5952006705186164 8 0.2907339057193292 1.348845482271832 9 0.21347640485600214 1.1142147971798138 >10 1.203488496367861 12.532141084888515 >50 0.08529571847302403 3.427607653322953 >100 0.031213149538288788 2.9156067929976732 >500 7.952394676655401E-4 0.32156071148201076 >1k 9.94049334581925E-4 1.1900859318455772 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3492 0.4013447116627876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2669 0.306755164784645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1758 0.20205154728040686 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1274 0.14642415883688187 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1129 0.1297589288279746 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.1493262075108468E-4 0.0 0.0 0.0 0.0 3 1.1493262075108468E-4 0.0 0.0 0.0 0.0 4 1.1493262075108468E-4 0.0 0.0 0.0 0.0 5 1.1493262075108468E-4 0.0 0.0 0.0 0.0 6 1.1493262075108468E-4 0.0 0.0 0.0 0.0 7 1.1493262075108468E-4 0.0 0.0 0.0 0.0 8 1.1493262075108468E-4 0.0 0.0 0.0 0.0 9 1.1493262075108468E-4 0.0 0.0 0.0 0.0 10-11 1.1493262075108468E-4 0.0 0.0 0.0 0.0 12-13 1.1493262075108468E-4 0.0 0.0 1.1493262075108468E-4 0.0 14-15 1.1493262075108468E-4 0.0 0.0 1.1493262075108468E-4 0.0 16-17 1.1493262075108468E-4 0.0 0.0 1.1493262075108468E-4 0.0 18-19 1.1493262075108468E-4 0.0 0.0 1.1493262075108468E-4 0.0 20-21 1.1493262075108468E-4 0.0 0.0 1.1493262075108468E-4 0.0 22-23 1.1493262075108468E-4 0.0 0.0 4.022641726287964E-4 0.0 24-25 1.1493262075108468E-4 0.0 0.0 5.746631037554234E-4 0.0 26-27 1.1493262075108468E-4 0.0 0.0 6.321294141309658E-4 0.0 28-29 3.44797862253254E-4 0.0 0.0 0.0013217251386374738 0.0 30-31 3.44797862253254E-4 0.0 0.0 0.0031031807602792862 0.0 32-33 3.44797862253254E-4 0.0 0.0 0.00540183317530098 0.0 34-35 3.44797862253254E-4 0.0 0.0 0.007298221417693877 0.0 36-37 3.44797862253254E-4 0.0 0.0 0.010229003246846537 0.0 38-39 4.597304830043387E-4 0.0 0.0 0.014826308076889925 0.0 40-41 4.597304830043387E-4 0.0 0.0 0.02264172628796368 0.0 42-43 5.17196793379881E-4 0.0 0.0 0.033732724190443354 0.0 44-45 8.045283452575927E-4 0.0 0.0 0.053903399132258716 0.0 46-47 9.194609660086774E-4 0.0 0.0 0.10010631267419476 0.0 48-49 9.194609660086774E-4 0.0 0.0 0.13895353848806136 0.0 50-51 9.194609660086774E-4 0.0 0.0 0.18509898571962186 0.0 52-53 9.769272763842198E-4 0.0 0.0 0.24681780306295434 0.0 54-55 0.001034393586759762 0.0 0.0 0.3254891819670718 0.0 56-57 0.0012642588282619314 0.0 0.0 0.420768324569721 0.0 58-59 0.0012642588282619314 0.0 0.0 0.5575381432635118 0.0 60-61 0.0012642588282619314 0.0 0.0 0.7299945407005144 0.0 62-63 0.0012642588282619314 0.0 0.0 0.9548602132000115 0.0 64-65 0.0012642588282619314 0.0 0.0 1.145303565784559 0.0 66-67 0.001379191449013016 0.0 0.0 1.4141884320317213 0.0 68-69 0.001379191449013016 0.0 0.0 1.737206562652645 0.0 70-71 0.001379191449013016 0.0 0.0 2.0060914288998077 0.0 72-73 0.0014366577593885586 0.0 0.0 2.4607648765910985 0.0 74-75 0.0014941240697641008 0.0 0.0 2.84452489727897 0.0 76-77 0.0014941240697641008 0.0 0.0 3.207309714679769 0.0 78-79 0.0014941240697641008 0.0 0.0 3.664971410510588 0.0 80-81 0.0016090566905151854 0.0 0.0 4.0144240439042616 0.0 82-83 0.0016090566905151854 0.0 0.0 4.440479269028532 0.0 84-85 0.0016090566905151854 0.0 0.0 5.157888687756802 0.0 86-87 0.0016090566905151854 0.0 0.0 5.960463178461627 0.0 88-89 0.0016090566905151854 0.0 0.0 6.967330402551505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3350 0.0 34.20707 1 GTATCAA 5025 0.0 32.172626 1 ACGCGTG 65 0.0058449744 29.229048 9 TGGACCG 90 8.918792E-4 26.402544 5 ATCAACG 6040 0.0 26.205997 3 CAACGCA 6115 0.0 25.880108 5 TCAACGC 6135 0.0 25.8002 4 TATCAAC 6170 0.0 25.730885 2 AACGCAG 6275 0.0 25.282839 6 TAGAGTG 205 2.6528141E-8 23.182724 5 GAGTACT 3630 0.0 22.767256 12-13 ACGCAGA 6910 0.0 22.684488 7 CGCAGAG 6945 0.0 22.570166 8 GTACATG 3740 0.0 22.503254 1 TACATGG 3645 0.0 22.429745 2 ACCGTGG 150 3.0321373E-5 22.166641 8 GTACTTT 3765 0.0 21.950901 14-15 ACATGGG 3645 0.0 21.777718 3 AGTACTT 3870 0.0 21.416698 12-13 GCAGAGT 7230 0.0 21.35075 9 >>END_MODULE