FastQCFastQC Report
Thu 26 May 2016
SRR937317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870075
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.5219090308306755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36420.41858460477545045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.2895152716719823No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17710.20354567135017096No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.10688733729850874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62750.030.3693161
GTGTACG1004.9353883E-528.5138341
GGTATCA42550.027.8102041
TAAGGTG4150.026.3274825
ATCAACG74900.025.4326823
GGACCGA3000.025.3310666
CAACGCA75800.025.1307135
TCAACGC75850.025.1141454
TATCAAC76450.024.9170422
AACGCAG77900.024.449036
GTAAGGT4100.023.1726534
GAGTACT42200.022.96134812-13
AGTACTT43000.022.5341612-13
GTACATG40850.022.4527551
AGGACCG3600.022.4324155
GTACTTT42700.022.35748114-15
ACGCAGA84650.022.1640857
TACATGG41100.022.076042
CGCAGAG85150.021.9223738
ATACCGT1302.9424936E-421.9211166
Produced by FastQC (version 0.11.5)