##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937317_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 870075 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47757032439732 33.0 31.0 34.0 31.0 34.0 2 32.69531132373646 34.0 31.0 34.0 31.0 34.0 3 32.76843835301555 34.0 31.0 34.0 31.0 34.0 4 36.299639686233945 37.0 37.0 37.0 35.0 37.0 5 36.23401890641611 37.0 35.0 37.0 35.0 37.0 6 36.31818176593972 37.0 37.0 37.0 35.0 37.0 7 36.286197166910895 37.0 37.0 37.0 35.0 37.0 8 36.26772174812516 37.0 37.0 37.0 35.0 37.0 9 38.03003074447605 39.0 38.0 39.0 35.0 39.0 10-11 37.96476912909807 39.0 38.0 39.0 35.0 39.0 12-13 37.90520644772002 39.0 38.0 39.0 35.0 39.0 14-15 39.20619659224779 40.0 38.5 41.0 36.0 41.0 16-17 39.18803723816912 40.0 38.0 41.0 36.0 41.0 18-19 39.20004022641726 40.0 38.5 41.0 36.0 41.0 20-21 39.100354567135014 40.0 38.5 41.0 36.0 41.0 22-23 38.9893032209867 40.0 38.0 41.0 35.5 41.0 24-25 38.855442346924114 40.0 38.0 41.0 35.0 41.0 26-27 38.81290176134242 40.0 38.0 41.0 35.0 41.0 28-29 38.72074706203489 40.0 38.0 41.0 35.0 41.0 30-31 38.79860414332098 40.0 38.0 41.0 35.0 41.0 32-33 38.72073901675143 40.0 38.0 41.0 35.0 41.0 34-35 38.60993822371634 40.0 38.0 41.0 35.0 41.0 36-37 38.46860672930495 40.0 38.0 41.0 35.0 41.0 38-39 38.339281671120304 40.0 38.0 41.0 34.5 41.0 40-41 38.1816987041347 40.0 38.0 41.0 34.0 41.0 42-43 37.96445651236962 40.0 38.0 41.0 34.0 41.0 44-45 37.649581932592014 40.0 37.5 41.0 33.0 41.0 46-47 37.25092836824412 40.0 37.0 41.0 32.5 41.0 48-49 36.912555814153954 40.0 37.0 41.0 31.5 41.0 50-51 36.67711461655604 40.0 37.0 41.0 31.0 41.0 52-53 36.44623681866506 40.0 36.0 41.0 31.0 41.0 54-55 36.22785966727007 39.0 36.0 41.0 30.5 41.0 56-57 35.98097347929776 39.0 35.5 41.0 30.0 41.0 58-59 35.739797718587475 39.0 35.0 40.0 29.0 41.0 60-61 35.419464988650404 38.5 35.0 40.0 28.5 41.0 62-63 35.05753297129558 38.0 35.0 40.0 28.0 41.0 64-65 34.705835703818636 37.5 34.0 40.0 28.0 41.0 66-67 34.301488952101835 37.0 34.0 39.5 26.5 41.0 68-69 33.8871775421659 36.0 34.0 39.0 26.0 40.5 70-71 33.4801844668563 36.0 33.5 39.0 26.0 40.0 72-73 33.04479613826395 35.0 33.0 38.0 25.0 40.0 74-75 32.60349222768152 35.0 33.0 37.0 24.5 39.5 76-77 31.288760164353647 34.0 30.5 36.0 22.5 38.5 78-79 31.87878631152487 35.0 32.0 36.0 24.0 39.0 80-81 31.717954774013734 35.0 32.0 36.0 23.0 37.5 82-83 31.41024509381375 35.0 32.0 35.5 22.0 37.0 84-85 31.084761083814612 35.0 32.0 35.0 20.5 37.0 86-87 30.752025974772287 34.0 31.5 35.0 19.5 36.0 88-89 30.4037031290406 34.0 31.0 35.0 17.5 36.0 90-91 30.03233054621728 34.0 31.0 35.0 12.5 35.5 92-93 29.72895325115651 34.0 31.0 35.0 6.0 35.0 94-95 29.378181191276614 34.0 30.0 35.0 2.0 35.0 96-97 28.968507312587995 34.0 30.0 35.0 2.0 35.0 98-99 28.572035169381948 34.0 29.5 35.0 2.0 35.0 100-101 26.7991736344568 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0021418002671111935 1101 2 0.07405788368802035 1101 3 -0.012572704257692635 1101 4 0.0245306365650535 1101 5 -0.01909222429716806 1101 6 -0.03788606510483561 1101 7 -0.08283591744201857 1101 8 -0.07143449149534575 1101 9 -0.026486087378202683 1101 10-11 -0.05938975201815566 1101 12-13 -0.043296157428208915 1101 14-15 -0.06733799057158762 1101 16-17 0.014095555836966867 1101 18-19 -0.03878277797092977 1101 20-21 0.014983900114906135 1101 22-23 -0.08245454688452014 1101 24-25 -0.037123085672739364 1101 26-27 -0.20429920988568995 1101 28-29 -0.16568486005085958 1101 30-31 -0.004217768919993148 1101 32-33 -0.09355088019579227 1101 34-35 -0.035546089314543394 1101 36-37 0.018674547593072077 1101 38-39 -0.13517880829176931 1101 40-41 -0.22574733624151122 1101 42-43 -0.060369961958762985 1101 44-45 -0.014160267208517041 1101 46-47 0.0949199490338728 1101 48-49 0.25822683100860644 1101 50-51 0.19045474015582187 1101 52-53 0.2566379929219167 1101 54-55 0.1554487372045159 1101 56-57 0.22049087965168468 1101 58-59 0.09290003701840988 1101 60-61 0.1576655088890746 1101 62-63 0.13378268124345993 1101 64-65 0.07984064793915024 1101 66-67 0.25240262609077746 1101 68-69 0.36047880879313965 1101 70-71 0.020827297377145726 1101 72-73 -0.08480504614401951 1101 74-75 -0.13281145970302077 1101 76-77 -0.22988735773498092 1101 78-79 -0.05820780031930184 1101 80-81 -0.032018925344381444 1101 82-83 -0.003955592334765612 1101 84-85 -0.04990523255524337 1101 86-87 -0.09605193696225811 1101 88-89 -0.006387217749807661 1101 90-91 -0.002970388864454776 1101 92-93 -0.15119682254637823 1101 94-95 -0.2284666891420244 1101 96-97 -0.3272622537010612 1101 98-99 0.08162940957111076 1101 100-101 0.5070118113580655 1102 1 0.060951242806218886 1102 2 0.13184006812853255 1102 3 0.10626577891120093 1102 4 0.0922603203147645 1102 5 -0.042623803273052374 1102 6 0.02612251705142654 1102 7 0.019409313338883294 1102 8 0.023933094610661954 1102 9 -0.0067920975966018204 1102 10-11 -0.0043298557192130716 1102 12-13 0.04173924938442042 1102 14-15 -0.1323651619817383 1102 16-17 -0.07053160256991475 1102 18-19 -0.07457995951279628 1102 20-21 -0.17691127188039246 1102 22-23 -0.09907272220106478 1102 24-25 -0.028278155050593057 1102 26-27 -0.2011177438756846 1102 28-29 -0.07744414790526122 1102 30-31 0.02860274806823071 1102 32-33 0.08047671923856825 1102 34-35 0.15945171638414735 1102 36-37 0.08637334858348567 1102 38-39 -0.029373120446031464 1102 40-41 0.02378400434114525 1102 42-43 0.014613919408098752 1102 44-45 0.10053721720111497 1102 46-47 0.0489013868701349 1102 48-49 0.24683786536431995 1102 50-51 0.1933525724028442 1102 52-53 0.28181963230881024 1102 54-55 0.13041771899452215 1102 56-57 0.05534261531693829 1102 58-59 0.11112492852240052 1102 60-61 0.10836091277597149 1102 62-63 0.08619722539766883 1102 64-65 -0.013342931101753663 1102 66-67 0.14061028826589705 1102 68-69 0.22270323434673145 1102 70-71 0.2133433753750822 1102 72-73 0.08710329165919006 1102 74-75 0.1616318108353667 1102 76-77 0.150078942131195 1102 78-79 0.34107972900577366 1102 80-81 0.30579457529765364 1102 82-83 0.18829256418122498 1102 84-85 0.08933727295759653 1102 86-87 0.15971772099763726 1102 88-89 0.1054281466938427 1102 90-91 0.019741442483173444 1102 92-93 0.2315275244458057 1102 94-95 0.09588599196910153 1102 96-97 0.08915193286331302 1102 98-99 0.22586571144195844 1102 100-101 0.20723124148988248 1103 1 0.001356238747973748 1103 2 -0.015743220060876695 1103 3 0.048021012415674136 1103 4 0.029119294874931256 1103 5 -0.08837432130352596 1103 6 0.01657292893352036 1103 7 -0.06558095377715034 1103 8 -0.05657584012032402 1103 9 -0.014360584018582756 1103 10-11 -0.09208525368776321 1103 12-13 -1.0367737319683101E-4 1103 14-15 -0.0623612135883036 1103 16-17 -0.09679595159031606 1103 18-19 -0.03820671894892058 1103 20-21 -0.023735891023633826 1103 22-23 -0.009554671012558913 1103 24-25 -0.0924241690250156 1103 26-27 -0.07147579222687028 1103 28-29 -0.2361114992112121 1103 30-31 -0.2106727185986088 1103 32-33 -0.04006917597019566 1103 34-35 -0.05942675156388333 1103 36-37 0.03677393161493825 1103 38-39 0.08822748669008007 1103 40-41 0.03563106780983816 1103 42-43 0.11030376776844975 1103 44-45 -0.023281638794266257 1103 46-47 -0.03940232932763621 1103 48-49 0.10840399028295877 1103 50-51 0.09696563486843246 1103 52-53 0.18019871950804145 1103 54-55 0.10837419627431188 1103 56-57 -0.0028798669231306917 1103 58-59 0.15379708067301578 1103 60-61 0.04359125351165005 1103 62-63 0.06561190902715452 1103 64-65 -0.005754671326343441 1103 66-67 0.18207971834763725 1103 68-69 0.276684803070431 1103 70-71 0.21486623745186506 1103 72-73 0.18844769137470507 1103 74-75 0.15533635173890303 1103 76-77 0.14555335509799505 1103 78-79 0.1768475910919669 1103 80-81 0.2411222134867188 1103 82-83 0.03003480376773382 1103 84-85 0.167601775143023 1103 86-87 0.2590850992330118 1103 88-89 0.1988870586070064 1103 90-91 0.05966007670312479 1103 92-93 0.24324276555831048 1103 94-95 0.32665577803397383 1103 96-97 0.11672540342581073 1103 98-99 -0.016054990505811162 1103 100-101 -0.04132294973915762 1104 1 0.12142533290676027 1104 2 0.13877548994157962 1104 3 0.15939110999917716 1104 4 0.10054658106989933 1104 5 -0.013907671830430957 1104 6 0.047123253112275165 1104 7 0.07005531825711131 1104 8 0.03481647915226915 1104 9 0.11867491070638891 1104 10-11 -0.004260850014738082 1104 12-13 0.10740723854424772 1104 14-15 0.04123541627213001 1104 16-17 -0.005629297918794407 1104 18-19 0.11609569694753219 1104 20-21 0.09375454255420834 1104 22-23 -0.027488713680597243 1104 24-25 0.05980902076200323 1104 26-27 0.09070842587556172 1104 28-29 0.05629901425674433 1104 30-31 0.13497051083358969 1104 32-33 0.037414720052687755 1104 34-35 -0.02930676643831731 1104 36-37 -0.22549897831675025 1104 38-39 -0.20875519178817825 1104 40-41 -0.19309151494420718 1104 42-43 -0.22539105736694864 1104 44-45 -0.08736392202033727 1104 46-47 -0.18143895762865014 1104 48-49 -0.11112852955462671 1104 50-51 -0.172186327297716 1104 52-53 -0.16596256617111038 1104 54-55 -0.14486834101673907 1104 56-57 -0.2156922750824819 1104 58-59 -0.11383819873248768 1104 60-61 -0.21473250840139002 1104 62-63 -0.2868455218936816 1104 64-65 -0.4265992754444028 1104 66-67 -0.2886111182794693 1104 68-69 -0.3454531423379805 1104 70-71 -0.21786142240335238 1104 72-73 -0.18787293970927266 1104 74-75 -0.22684521235612465 1104 76-77 -0.11209603403047197 1104 78-79 -0.22477157833866457 1104 80-81 -0.25285678836658576 1104 82-83 -0.09043576523946228 1104 84-85 -0.12995518855255384 1104 86-87 -0.08995885832686312 1104 88-89 -0.1096472995430453 1104 90-91 -0.04627680290390401 1104 92-93 -0.2688509613878125 1104 94-95 -0.04087738956134501 1104 96-97 -0.023474786703079786 1104 98-99 0.10790289924342389 1104 100-101 0.3490466770204357 1105 1 0.02586448082870163 1105 2 0.059265583789340326 1105 3 -0.0016955874535256044 1105 4 0.04879065972325236 1105 5 0.10971440473742433 1105 6 0.09710090840796681 1105 7 0.13752701496498787 1105 8 0.08105139647686599 1105 9 0.0018772792450434395 1105 10-11 -0.08097301298212045 1105 12-13 0.00793140806607795 1105 14-15 0.11867843727614513 1105 16-17 0.133294245951312 1105 18-19 0.1375605785205991 1105 20-21 0.06919398840553725 1105 22-23 0.1487411760855224 1105 24-25 -0.13287681269546425 1105 26-27 0.0048907959038615445 1105 28-29 0.17109958777081857 1105 30-31 0.06608364951971879 1105 32-33 0.01800741333438083 1105 34-35 -0.00919187741701677 1105 36-37 -0.03560157775068973 1105 38-39 -0.008299918437955967 1105 40-41 0.026007621060564645 1105 42-43 -0.32542550545274906 1105 44-45 -0.046603457250490976 1105 46-47 -0.10675178348892445 1105 48-49 -0.14996176357815472 1105 50-51 -0.08915247507069068 1105 52-53 -0.01482857242762492 1105 54-55 -4.005173909433779E-4 1105 56-57 -0.015618723764141862 1105 58-59 0.21673308087424914 1105 60-61 -0.022204949932749685 1105 62-63 0.07830884277188943 1105 64-65 -0.09334197617432949 1105 66-67 0.01892184015938625 1105 68-69 0.04866089013631836 1105 70-71 0.016069011083907014 1105 72-73 0.0020942880577479173 1105 74-75 -0.011135023170957936 1105 76-77 0.0903516421202788 1105 78-79 -0.0030795256051625586 1105 80-81 -0.18885783111945642 1105 82-83 -0.05419079279792882 1105 84-85 -0.059597300871931935 1105 86-87 -0.08713783940834574 1105 88-89 0.022107318362511563 1105 90-91 0.19915184300701583 1105 92-93 0.17845743277277037 1105 94-95 0.16936765736253534 1105 96-97 -0.021077386137015708 1105 98-99 0.1501297352332891 1105 100-101 0.4019477644627081 1106 1 -0.10565304117237417 1106 2 0.008040147710737244 1106 3 -0.03947592369930675 1106 4 0.0022828371197149977 1106 5 0.05358801291330906 1106 6 -0.10309702238722451 1106 7 -0.05915981278001681 1106 8 -0.09965311098299878 1106 9 -0.13433025631941575 1106 10-11 -0.056451477165317954 1106 12-13 -0.10789654985588726 1106 14-15 -0.051843674195950484 1106 16-17 -0.01097542095718751 1106 18-19 0.0071559243869288025 1106 20-21 -0.0015978136321237457 1106 22-23 0.08849155459859759 1106 24-25 0.13264025193564066 1106 26-27 0.19276635184493784 1106 28-29 0.21675674166792902 1106 30-31 0.18171347950426053 1106 32-33 0.1612125122209207 1106 34-35 0.06365316090743534 1106 36-37 0.1916255379796823 1106 38-39 0.12205150729462133 1106 40-41 -0.042262731902880546 1106 42-43 -0.10327283062702719 1106 44-45 0.010906873002170414 1106 46-47 0.20253282680032925 1106 48-49 0.1448486644570437 1106 50-51 -0.07920172119882096 1106 52-53 0.013889135675704267 1106 54-55 0.11229977911096967 1106 56-57 0.22380952435117507 1106 58-59 0.2974529654277589 1106 60-61 0.17302499675999172 1106 62-63 0.3907526572373996 1106 64-65 0.32498298518579816 1106 66-67 0.32698909720130587 1106 68-69 0.5054709712011984 1106 70-71 0.30632939653639113 1106 72-73 0.2122380441691547 1106 74-75 -0.11113147461089312 1106 76-77 0.18634906349996072 1106 78-79 0.5514943711869407 1106 80-81 0.5262006806933073 1106 82-83 0.3507984826649633 1106 84-85 0.46244911429382185 1106 86-87 0.4045723299762578 1106 88-89 0.3697508773316933 1106 90-91 0.32492266911180323 1106 92-93 0.5191162426645768 1106 94-95 -0.09900008386069103 1106 96-97 0.13254076514204272 1106 98-99 0.6596507190518572 1106 100-101 1.2252629869968565 1107 1 0.029672933343562136 1107 2 0.0756885309209494 1107 3 0.022223744058607053 1107 4 -0.01287373044225859 1107 5 -0.04065920927510547 1107 6 -0.009740647111875944 1107 7 0.02197784254011026 1107 8 0.027560315004905078 1107 9 0.07109027940573753 1107 10-11 0.109089055152225 1107 12-13 0.1390347746095486 1107 14-15 0.16173443920447994 1107 16-17 0.15842179697171588 1107 18-19 0.11659500725272665 1107 20-21 0.14767502649007014 1107 22-23 0.14111724068459353 1107 24-25 0.14966978862222646 1107 26-27 0.1363496983624799 1107 28-29 0.09374441341761752 1107 30-31 0.09295372825987869 1107 32-33 0.17498656326207396 1107 34-35 0.2144874171354516 1107 36-37 -0.005980615192434868 1107 38-39 0.1520859485072492 1107 40-41 0.0340142095227165 1107 42-43 0.29034763451189605 1107 44-45 0.2756928628523596 1107 46-47 0.4147892738789096 1107 48-49 0.43382017439387965 1107 50-51 0.37131976500644015 1107 52-53 0.4107760394958788 1107 54-55 0.48210591037701533 1107 56-57 0.5096655041002265 1107 58-59 0.6059926823526354 1107 60-61 0.6628143333711236 1107 62-63 0.5315880087690061 1107 64-65 0.4256048742193741 1107 66-67 0.5226991581732037 1107 68-69 0.4654936074752243 1107 70-71 0.5741639813554826 1107 72-73 0.6543500328753566 1107 74-75 0.6993603166972733 1107 76-77 0.48994287625172817 1107 78-79 0.5811734472375178 1107 80-81 0.5411759146623183 1107 82-83 0.6487137548479041 1107 84-85 0.561540012321057 1107 86-87 0.6143546079643052 1107 88-89 0.7790402687080054 1107 90-91 0.5675774415929098 1107 92-93 0.7875617634814311 1107 94-95 0.6542523592747962 1107 96-97 0.6894490882322586 1107 98-99 0.7915556953811311 1107 100-101 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66-67 -0.13026887060768644 2316 68-69 0.07173003158600011 2316 70-71 0.1829849153766574 2316 72-73 -0.07940641567202533 2316 74-75 -0.1462071554721618 2316 76-77 0.1344667019373098 2316 78-79 0.025157631326578667 2316 80-81 0.0825399521317749 2316 82-83 -0.19944501517653634 2316 84-85 -0.5519442396474439 2316 86-87 -0.5943950526384647 2316 88-89 -0.3337380370210212 2316 90-91 -0.2716653631858037 2316 92-93 -0.25042187757205525 2316 94-95 -0.2599734756423935 2316 96-97 -0.17519420527846563 2316 98-99 -0.17772037032617405 2316 100-101 -0.8996004410263829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 2.0 5 14.0 6 26.0 7 52.0 8 116.0 9 262.0 10 472.0 11 855.0 12 1576.0 13 2290.0 14 2892.0 15 4181.0 16 6201.0 17 9797.0 18 8912.0 19 4633.0 20 3400.0 21 3325.0 22 3347.0 23 3586.0 24 3845.0 25 4484.0 26 5259.0 27 6485.0 28 8189.0 29 10473.0 30 13297.0 31 17130.0 32 23180.0 33 31868.0 34 46668.0 35 72670.0 36 125548.0 37 216586.0 38 205323.0 39 23128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15206879381978 19.948298840978687 15.543005911825144 21.356626453376386 2 15.562106714938368 21.189322759532224 39.92517886389104 23.323391661638365 3 19.213171278338073 25.430911128351003 30.65103583024452 24.704881763066403 4 12.865097836393414 17.58400137919145 37.671235238341524 31.879665546073614 5 13.30087880128975 38.289872231833414 34.15875898774046 14.250489979136372 6 31.38959285119099 37.200241358503575 17.27759101226906 14.132574778036378 7 27.775766456914635 33.88880268942332 20.84762807803925 17.48780277562279 8 26.352900612016207 35.119041461942935 19.64727178691492 18.880786139125938 9 26.826423009510673 16.662931356492255 20.42674482084878 36.08390081314829 10-11 24.78521966497141 27.31569117604804 27.25523661753297 20.643852541447576 12-13 25.801944965811956 24.83222055052578 27.917017703763385 21.448816779898877 14-15 22.971180645346664 25.905927649915238 26.71982300376404 24.403068700974053 16-17 21.572967847599344 29.26121311381203 27.355400396517542 21.810418642071085 18-19 21.47688417665144 27.60342499209838 30.383415222825615 20.53627560842456 20-21 22.432031721403327 26.518805850070397 30.385081745826508 20.66408068269977 22-23 22.127823415999334 26.341117212170317 30.32339444928123 21.207664922549114 24-25 21.96909574984671 26.479514110573287 30.464057962852607 21.087332176727394 26-27 21.573794985992386 26.731527907477908 30.59636520364916 21.09831190288054 28-29 21.50508653020598 27.05699603352938 30.57814383886773 20.85977359739691 30-31 21.613653995345228 26.750280148263077 30.324282389449188 21.311783466942504 32-33 21.79415567623481 26.678792058155903 30.103324426055224 21.42372783955406 34-35 22.092865557566878 26.75051001350458 30.17613424130104 20.980490187627503 36-37 22.293480447087894 26.438640347096516 30.230324971985173 21.037554233830416 38-39 21.793925810993304 26.89038301295865 30.13780421228055 21.17788696376749 40-41 22.01402177973163 26.985259891388676 29.923972071373157 21.07674625750654 42-43 21.83002944559175 27.230073932978087 29.49151607149939 21.448380549930768 44-45 22.171422003850243 26.9823865758699 29.46837916271586 21.377812257564003 46-47 22.4793264948424 27.527741861333794 28.76102634830331 21.2319052955205 48-49 22.407321207941845 27.524236416400882 28.604488118840333 21.46395425681694 50-51 22.36715225698934 27.829439990805387 28.43174438985145 21.37166336235382 52-53 22.590983535902076 27.929431370858833 28.02258425997759 21.4570008332615 54-55 22.332787403384767 28.001321725138638 27.99120765451254 21.674683216964056 56-57 22.37945004740971 28.09838232336293 28.03649110708847 21.485676522138895 58-59 22.45352412148378 27.540671781168292 28.077636985317355 21.92816711203057 60-61 22.251300175272245 27.831968508461912 28.540240783840474 21.376490532425365 62-63 22.022871591529466 27.567853345975923 28.84423756572709 21.56503749676752 64-65 21.95758986294285 27.790880096543404 28.54972272505244 21.70180731546131 66-67 21.982070511162828 27.72088613050599 28.251759905755254 22.04528345257593 68-69 22.014711375456137 27.652328822227968 28.404275493491944 21.92868430882395 70-71 22.13406890210614 27.723012383989886 28.258885728241818 21.884032985662156 72-73 22.06407493606873 27.416831882308994 28.337959371318565 22.18113381030371 74-75 22.060282159583945 28.129241732034593 27.75025141510789 22.060224693273568 76-77 22.02361865356435 27.666695399821855 28.141999252937964 22.167686693675833 78-79 22.1799844840962 27.480332155273974 27.956383070424963 22.383300290204865 80-81 22.205224954874552 27.87732544741858 27.82187042603823 22.095579171668632 82-83 21.896163825428342 27.958187392429938 27.77780012585158 22.36784865629014 84-85 21.9349633302416 27.795860759508216 27.894589242388196 22.37458666786198 86-87 21.799614975720484 27.77450219808637 27.819153521248168 22.606729304944974 88-89 21.673878688618796 27.47895296382496 27.788006781024627 23.05916156653162 90-91 21.720284012731604 27.169034014168453 27.959816589479296 23.150865383620644 92-93 21.711041627277915 27.121375205228563 28.006882232564983 23.16070093492854 94-95 21.40097118064535 27.342585409303794 27.976036548573397 23.28040686147746 96-97 21.357066919518434 27.335344654196476 27.932764416860618 23.374824009424476 98-99 21.13603046179432 27.871266048213165 27.53537488499535 23.457328604997162 100-101 20.843331875342344 28.278166287991347 27.24697773412107 23.63152410254524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 141.0 1 103.0 2 80.5 3 107.0 4 107.0 5 105.5 6 183.0 7 414.0 8 697.0 9 886.0 10 973.5 11 989.5 12 1071.0 13 1205.5 14 1346.0 15 1464.0 16 1490.0 17 1593.0 18 1736.0 19 1782.5 20 1894.0 21 2103.5 22 2414.5 23 2831.5 24 3223.0 25 3912.5 26 5029.5 27 6523.0 28 7945.5 29 9291.0 30 11032.0 31 13047.0 32 14869.0 33 16357.0 34 18038.5 35 19969.0 36 22301.5 37 24266.5 38 26022.5 39 26997.5 40 27441.5 41 28611.5 42 29235.0 43 30180.5 44 32034.5 45 32639.5 46 32668.0 47 34338.5 48 36492.5 49 35060.0 50 32033.0 51 30412.5 52 29044.5 53 28372.0 54 27524.0 55 25209.0 56 21941.0 57 19246.5 58 17634.0 59 15578.5 60 12420.5 61 9797.5 62 7797.0 63 5699.5 64 3961.0 65 2846.5 66 2032.0 67 1540.0 68 1161.5 69 781.5 70 535.0 71 356.5 72 264.5 73 225.5 74 156.5 75 101.5 76 75.0 77 50.5 78 36.0 79 27.0 80 19.0 81 8.0 82 4.5 83 5.0 84 1.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05309887078700112 2 0.0 3 0.0 4 0.0 5 0.016090566905151853 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003907709105536879 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.619946556331351E-4 24-25 5.746631037554233E-5 26-27 0.0014366577593885586 28-29 0.0036778438640347093 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.019021348734304513 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.746631037554233E-5 82-83 2.873315518777117E-4 84-85 0.001379191449013016 86-87 0.0 88-89 0.0 90-91 0.013102318765623651 92-93 9.769272763842198E-4 94-95 0.0 96-97 0.0 98-99 1.72398931126627E-4 100-101 0.01798695514754475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 870075.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.85572447324093 #Duplication Level Percentage of deduplicated Percentage of total 1 76.30349855948455 41.856836933234206 2 12.98878745197535 14.250186914140977 3 4.377780652687438 7.204389878643208 4 2.0170912797341036 4.425960139138837 5 1.0964496844814386 3.007327089534287 6 0.7122708313197873 2.344327948392271 7 0.46097610780765014 1.7701024851050424 8 0.3433161820991031 1.5066286313946728 9 0.23961562398692277 1.1829859784019303 >10 1.3247856262879936 13.112681784967172 >50 0.0904277377859563 3.4113380166942084 >100 0.04331011165902762 4.1453785449086125 >500 8.450753453609611E-4 0.33632163330733233 >1k 8.450753453609611E-4 1.4455340221372694 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4541 0.5219090308306755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3642 0.41858460477545045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2519 0.2895152716719823 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1771 0.20354567135017096 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 930 0.10688733729850874 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1493262075108468E-4 0.0 14-15 0.0 0.0 0.0 1.1493262075108468E-4 0.0 16-17 0.0 0.0 0.0 1.1493262075108468E-4 0.0 18-19 0.0 0.0 0.0 1.1493262075108468E-4 0.0 20-21 0.0 0.0 0.0 1.1493262075108468E-4 0.0 22-23 0.0 0.0 0.0 2.873315518777117E-4 0.0 24-25 0.0 0.0 0.0 4.597304830043387E-4 0.0 26-27 0.0 0.0 0.0 5.746631037554234E-4 0.0 28-29 0.0 0.0 0.0 0.001379191449013016 0.0 30-31 0.0 0.0 0.0 0.0033330460017814553 0.0 32-33 0.0 0.0 0.0 0.005804097347929776 0.0 34-35 0.0 0.0 0.0 0.007757951900698215 0.0 36-37 0.0 0.0 0.0 0.01057380110909979 0.0 38-39 0.0 0.0 0.0 0.015056173318392093 0.0 40-41 0.0 0.0 0.0 0.023043990460592478 0.0 42-43 5.746631037554234E-5 0.0 0.0 0.03413498836307215 0.0 44-45 1.1493262075108468E-4 0.0 0.0 0.05453552854638968 0.0 46-47 1.72398931126627E-4 0.0 0.0 0.10171536936470994 0.0 48-49 2.2986524150216936E-4 0.0 0.0 0.1407349941097032 0.0 50-51 2.2986524150216936E-4 0.0 0.0 0.18653564347901042 0.0 52-53 2.2986524150216936E-4 0.0 0.0 0.24888659023647386 0.0 54-55 2.2986524150216936E-4 0.0 0.0 0.3282475648650979 0.0 56-57 2.2986524150216936E-4 0.0 0.0 0.42392897164037585 0.0 58-59 2.2986524150216936E-4 0.0 0.0 0.5611010545067954 0.0 60-61 2.2986524150216936E-4 0.0 0.0 0.7343619802890555 0.0 62-63 2.2986524150216936E-4 0.0 0.0 0.959687383271557 0.0 64-65 2.2986524150216936E-4 0.0 0.0 1.1523144556503748 0.0 66-67 2.2986524150216936E-4 0.0 0.0 1.423268109071057 0.0 68-69 2.2986524150216936E-4 0.0 0.0 1.747607964830618 0.0 70-71 2.2986524150216936E-4 0.0 0.0 2.0173548257334137 0.0 72-73 3.44797862253254E-4 0.0 0.0 2.477774904462259 0.0 74-75 4.597304830043387E-4 0.0 0.0 2.8659598310490475 0.0 76-77 4.597304830043387E-4 0.0 0.0 3.2309858345544926 0.0 78-79 4.597304830043387E-4 0.0 0.0 3.693359767836106 0.0 80-81 4.597304830043387E-4 0.0 0.0 4.049076229060713 0.0 82-83 5.746631037554234E-4 0.0 0.0 4.478694365428268 0.0 84-85 6.321294141309658E-4 0.0 0.0 5.20639025371376 0.0 86-87 6.89595724506508E-4 0.0 0.0 6.022756658908715 0.0 88-89 6.89595724506508E-4 0.0 0.0 7.049219894836652 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6275 0.0 30.369316 1 GTGTACG 100 4.9353883E-5 28.513834 1 GGTATCA 4255 0.0 27.810204 1 TAAGGTG 415 0.0 26.327482 5 ATCAACG 7490 0.0 25.432682 3 GGACCGA 300 0.0 25.331066 6 CAACGCA 7580 0.0 25.130713 5 TCAACGC 7585 0.0 25.114145 4 TATCAAC 7645 0.0 24.917042 2 AACGCAG 7790 0.0 24.44903 6 GTAAGGT 410 0.0 23.172653 4 GAGTACT 4220 0.0 22.961348 12-13 AGTACTT 4300 0.0 22.53416 12-13 GTACATG 4085 0.0 22.452755 1 AGGACCG 360 0.0 22.432415 5 GTACTTT 4270 0.0 22.357481 14-15 ACGCAGA 8465 0.0 22.164085 7 TACATGG 4110 0.0 22.07604 2 CGCAGAG 8515 0.0 21.922373 8 ATACCGT 130 2.9424936E-4 21.921116 6 >>END_MODULE