FastQCFastQC Report
Thu 26 May 2016
SRR937316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157602
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43750.3779364582991391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.26917714378516966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20460.1767446842697231No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16840.14547314189160004No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14730.1272458064170587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42800.032.8647581
GTATCAA64250.030.9161661
TAGCGCG705.805887E-727.14525628-29
ATCAACG77300.025.4431953
TATCAAC78050.025.3215352
TCAACGC78750.024.9154724
CAACGCA78700.024.8687825
ACGCAGT1151.280612E-424.7858756
AACGCAG80350.024.4182746
GTACATG54450.023.7385121
GAGTACT41450.023.43693412-13
TACATGG53400.023.3982892
ACATGGG52500.022.7125173
GTACTTT43350.022.08000414-15
ACGCAGA89500.021.7626447
CGCAGAG89150.021.7415078
AGTACTT43100.020.66598112-13
GGCGACA1856.5446784E-620.5432476
GCAGAGT94100.020.244439
GCGACAG1908.241914E-620.0026347
Produced by FastQC (version 0.11.5)