FastQCFastQC Report
Thu 26 May 2016
SRR937316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157602
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58340.5039728680496405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46810.4043704140110332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.26943630021371767No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21610.18667901403072903No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.10504473903811501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.030.192381
ACCGTCG650.005842608429.2322858
GTATCAA80250.028.7223011
CCGTCGT700.00838947427.1442649
ATCAACG97950.023.4693343
TATCAAC98200.023.457952
TCAACGC99200.023.1736014
CAACGCA99050.023.1607445
GTACATG51400.022.652991
AACGCAG101400.022.623986
GTAAGGT4450.022.4139864
GAGTACT54750.021.90752812-13
TACATGG51350.021.7363822
GGTGTGC8150.021.5655358
AGAGTAC85850.021.41345610-11
TAAGGTG5200.021.0079825
AGTACTT58300.020.81797612-13
GTACTTT57400.020.7692914-15
ACATGGG52450.020.5560763
ACGCAGA111200.020.5046627
Produced by FastQC (version 0.11.5)