FastQCFastQC Report
Thu 26 May 2016
SRR937315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179392
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.9660408490902603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.8885569033178737No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10480.5841955048162683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.5479620049946486No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9610.5356983589011773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6590.3673519443453443No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6340.3534159828754906No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4570.2547493756689262No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.23189439885836602No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3780.21071173742418836No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3680.20513735283624687No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3660.20402247591865857No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC3540.1973332144131288No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3520.1962183374955405No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3500.1951034605779522No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3350.18674188369603997No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3260.18172493756689262No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT3120.17392079914377453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.16890385301462718No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3010.16778897609703888No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.16778897609703888No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2960.16500178380306815No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2950.164444345344274No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA2840.15831252229753834No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT2790.1555253300035676No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2780.15496789154477347No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC2670.14883606849803782No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA2670.14883606849803782No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA2660.14827863003924366No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC2620.14604887620406706No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2430.13545754548697825No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG2400.1337852301105958No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2220.12375133785230111No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2170.12096414555833035No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2140.11929183018194792No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA2110.11761951480556547No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2080.11594719942918301No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA2060.11483232251159473No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT1990.11093025330003567No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT1950.10870049946485909No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1940.10814306100606494No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA1930.10758562254727078No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT1910.10647074562968248No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA1900.10591330717088833No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA1900.10591330717088833No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG1830.10201123795932929No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT1830.10201123795932929No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC1830.10201123795932929No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA1820.10145379950053514No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG301.2988377E-463.3273664
TAGTCGA301.2988377E-463.3273665
GTCGAAT405.378394E-447.495527
AGTCGAA405.378394E-447.495526
GGTACTC459.607683E-442.218243
CCTAGTC459.607683E-442.218243
CATATAG500.001612911537.9964183
TACTCCC500.001612911537.9964185
CAACCGT905.6663157E-736.940966
ACAACCG958.651732E-734.99675
AACCGTC958.651732E-734.99677
GTGGTAC550.002571477634.551871
ACCGTCT1001.2920191E-633.2468648
CCCTATA902.3986071E-531.6725442
GTTATTC753.0698234E-431.6636833
CCGTCTC953.4767734E-529.9971719
GGTTATT804.473759E-429.693012
ACTTAGA650.00583372829.2280147
CTTAGAT650.00583372829.2280148
TAGATCA856.389375E-427.9385455
Produced by FastQC (version 0.11.5)