##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937315_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179392 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98147632001427 33.0 31.0 34.0 31.0 34.0 2 32.130763913663934 33.0 31.0 34.0 31.0 34.0 3 32.11042298430253 34.0 31.0 34.0 30.0 34.0 4 35.74528964502319 37.0 35.0 37.0 35.0 37.0 5 35.75728014627185 37.0 35.0 37.0 35.0 37.0 6 35.83653117195862 37.0 35.0 37.0 35.0 37.0 7 35.79662415269354 37.0 35.0 37.0 35.0 37.0 8 35.81131823046736 37.0 35.0 37.0 35.0 37.0 9 37.43138490010703 39.0 37.0 39.0 35.0 39.0 10-11 37.35571262932572 39.0 37.0 39.0 35.0 39.0 12-13 37.26311095255084 39.0 37.0 39.0 35.0 39.0 14-15 38.391410988226895 40.0 38.0 41.0 34.0 41.0 16-17 38.326633852122725 40.0 38.0 41.0 34.0 41.0 18-19 38.35169071084552 40.0 38.0 41.0 34.0 41.0 20-21 38.304497970924004 40.0 38.0 41.0 34.0 41.0 22-23 38.350651088119875 40.0 38.0 41.0 34.0 41.0 24-25 38.32309690510168 40.0 38.0 41.0 34.0 41.0 26-27 38.218005819657506 40.0 38.0 41.0 34.0 41.0 28-29 38.10472317606136 40.0 38.0 41.0 34.0 41.0 30-31 37.83974759186586 40.0 37.5 41.0 34.0 41.0 32-33 37.53921579557617 40.0 36.5 41.0 33.0 41.0 34-35 37.46543881555476 40.0 36.0 41.0 33.0 41.0 36-37 37.28708916785587 40.0 36.0 41.0 33.0 41.0 38-39 37.11974335533357 40.0 36.0 41.0 33.0 41.0 40-41 36.90904276667856 39.5 36.0 41.0 32.5 41.0 42-43 36.592858098465925 39.0 35.0 41.0 31.0 41.0 44-45 36.22507692650731 39.0 35.0 41.0 30.5 41.0 46-47 35.67195582857653 39.0 35.0 41.0 29.0 41.0 48-49 35.213426462718516 39.0 35.0 40.0 26.5 41.0 50-51 34.218234369425616 38.0 34.0 39.5 23.5 40.5 52-53 34.30655770602925 38.0 34.5 39.5 23.5 40.5 54-55 34.60814863539065 39.0 35.0 40.0 23.0 41.0 56-57 34.369815822333216 39.0 35.0 40.0 20.5 41.0 58-59 34.132324741348555 38.0 34.5 40.0 18.5 41.0 60-61 33.81237457634677 38.0 34.0 40.0 12.5 41.0 62-63 33.454763869068856 38.0 34.0 40.0 8.0 41.0 64-65 33.06828899839458 37.5 33.0 40.0 4.0 41.0 66-67 32.60809010435248 37.0 33.0 39.5 2.0 41.0 68-69 32.107298541740995 36.5 32.0 39.0 2.0 40.5 70-71 31.635089636104176 36.0 31.5 39.0 2.0 40.0 72-73 31.14406996967535 35.5 31.0 38.0 2.0 40.0 74-75 30.567310693899394 35.0 30.5 37.5 2.0 39.5 76-77 30.069222707813058 35.0 30.0 37.0 2.0 39.0 78-79 29.508475851765965 34.5 29.0 36.5 2.0 39.0 80-81 28.908465817873708 34.0 29.0 36.0 2.0 38.0 82-83 28.359472551730292 34.0 28.5 35.5 2.0 37.0 84-85 27.796900084730645 34.0 26.5 35.0 2.0 36.5 86-87 27.26926786032822 34.0 26.0 35.0 2.0 36.0 88-89 26.69973298697824 33.0 25.5 35.0 2.0 36.0 90-91 26.13609023813771 33.0 24.5 35.0 2.0 35.0 92-93 25.52531328041384 32.0 21.0 35.0 2.0 35.0 94-95 24.78428246521584 32.0 16.5 34.0 2.0 35.0 96-97 23.956809668212628 31.5 2.0 34.0 2.0 35.0 98-99 22.833075053514094 31.0 2.0 34.0 2.0 35.0 100-101 19.713086425258652 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13450158940781165 1101 2 0.34311542826022645 1101 3 0.2873979522612089 1101 4 0.09067091387230874 1101 5 -0.08565174421944022 1101 6 0.1935009234296956 1101 7 -0.02318053143849852 1101 8 0.09144445823246627 1101 9 0.07582912948974752 1101 10-11 0.1148407231236348 1101 12-13 0.3035417561945408 1101 14-15 0.41356029366306046 1101 16-17 0.5322126501489564 1101 18-19 0.5910637571695077 1101 20-21 0.3965572742568213 1101 22-23 0.35999110043207594 1101 24-25 0.4081077041479588 1101 26-27 0.49466057536457697 1101 28-29 0.10533419114466369 1101 30-31 0.19375429032788816 1101 32-33 0.2827174392833598 1101 34-35 0.46026740785831066 1101 36-37 0.006128552748791094 1101 38-39 -0.30743761668122005 1101 40-41 0.0941732582910717 1101 42-43 0.05537645022646842 1101 44-45 -0.20770470987314127 1101 46-47 -0.3419807355231228 1101 48-49 -0.3067021951833695 1101 50-51 -0.35749907901418965 1101 52-53 -0.2950017587020639 1101 54-55 -0.1544242135870988 1101 56-57 0.07374985185206384 1101 58-59 -0.11522864330746074 1101 60-61 0.4280402720619705 1101 62-63 0.8981381013171728 1101 64-65 0.3315518804656463 1101 66-67 0.8472713419228839 1101 68-69 0.3473806373542594 1101 70-71 0.9191460176313413 1101 72-73 0.9856782720396993 1101 74-75 0.5558027506565502 1101 76-77 0.8003110711224863 1101 78-79 0.8171189085733985 1101 80-81 0.9809264646042379 1101 82-83 0.8207723598000207 1101 84-85 0.8211723284579975 1101 86-87 0.7354247520656827 1101 88-89 1.0929427520326378 1101 90-91 1.326360956185603 1101 92-93 1.5036197113868894 1101 94-95 1.8131304311058294 1101 96-97 1.6984258331237179 1101 98-99 2.222880033584122 1101 100-101 2.6392952643583563 1102 1 0.08241825607447595 1102 2 0.011313957671994501 1102 3 -0.2760211653858491 1102 4 0.03683290139458961 1102 5 -0.1326938565189053 1102 6 -0.09075709796067599 1102 7 -0.23911747439750286 1102 8 -0.2662315667229649 1102 9 0.23725961077317237 1102 10-11 -0.22998158079792574 1102 12-13 0.04925254050826311 1102 14-15 0.11917805213007426 1102 16-17 0.3109977437318463 1102 18-19 0.263147647543839 1102 20-21 0.40736386962223037 1102 22-23 0.027785775120129586 1102 24-25 0.20015037348842668 1102 26-27 -0.0523119068101181 1102 28-29 0.49877224818565935 1102 30-31 0.4666486753546266 1102 32-33 0.32974562555786946 1102 34-35 0.358092162760272 1102 36-37 0.553295999272855 1102 38-39 0.054820070484559835 1102 40-41 0.4664437573998086 1102 42-43 0.39039483257941043 1102 44-45 0.5973309407507514 1102 46-47 0.9337684846194776 1102 48-49 0.457001013372782 1102 50-51 0.9463110814136115 1102 52-53 1.0067334240073862 1102 54-55 1.0036500956820547 1102 56-57 1.208205622796811 1102 58-59 1.4129437051773834 1102 60-61 1.289337063505819 1102 62-63 0.9578250442761771 1102 64-65 0.8098829856349923 1102 66-67 0.819112919462988 1102 68-69 1.1873009804915107 1102 70-71 1.137325050608169 1102 72-73 1.1868536285459328 1102 74-75 0.73395838791145 1102 76-77 0.5964062137249826 1102 78-79 0.8465585253291934 1102 80-81 0.8178248603261622 1102 82-83 1.1504988526698945 1102 84-85 1.249202631488302 1102 86-87 1.0186800996592638 1102 88-89 0.9108042716404796 1102 90-91 0.5870422173264274 1102 92-93 0.918865923508104 1102 94-95 1.1375789605175974 1102 96-97 0.9773919650310248 1102 98-99 0.7252196057766334 1102 100-101 0.9292490299555034 1103 1 0.43098968464590115 1103 2 0.34677614254594147 1103 3 0.39355866654692306 1103 4 0.30421258053897304 1103 5 0.2908661129234176 1103 6 0.4590366377154069 1103 7 0.41286113522816237 1103 8 0.4856706487086626 1103 9 0.47261484377546026 1103 10-11 0.3626234612188739 1103 12-13 0.43395842286120256 1103 14-15 0.4520275555678168 1103 16-17 0.41422157872037957 1103 18-19 0.5494119714552284 1103 20-21 0.5295334647330066 1103 22-23 0.3701845528130292 1103 24-25 0.4057118708146277 1103 26-27 0.7371456944121917 1103 28-29 -0.003534856474388448 1103 30-31 0.41036143318503093 1103 32-33 0.3810061297595482 1103 34-35 0.604478717382122 1103 36-37 0.45349462417736675 1103 38-39 0.36412488331878023 1103 40-41 0.7055423059101216 1103 42-43 0.23711752165503697 1103 44-45 0.46110481393638736 1103 46-47 0.1821412882863953 1103 48-49 0.23365494767377726 1103 50-51 -0.10857050758561826 1103 52-53 0.5515309793931777 1103 54-55 0.5878823340319386 1103 56-57 0.5079611613758814 1103 58-59 0.9482683805020642 1103 60-61 0.09554027206197446 1103 62-63 -0.048439279635204 1103 64-65 -0.0351743100105395 1103 66-67 -0.10246080093426002 1103 68-69 0.40293123259235486 1103 70-71 0.4021073271551501 1103 72-73 0.21886279584921908 1103 74-75 0.2584962030374989 1103 76-77 0.37778130921772757 1103 78-79 0.41893438476387956 1103 80-81 0.14949789317566697 1103 82-83 0.4632872407523969 1103 84-85 0.46426756655323587 1103 86-87 0.8531033234942527 1103 88-89 0.3049219186993035 1103 90-91 0.4568817895189383 1103 92-93 0.6991256637678411 1103 94-95 0.5244697168201142 1103 96-97 0.39937821407609775 1103 98-99 -0.3960485378444467 1103 100-101 -0.5849755689749792 1104 1 0.24284606356111027 1104 2 0.26799844965059805 1104 3 -0.09420298356766921 1104 4 -0.03803340876584116 1104 5 0.18816175845435623 1104 6 0.20870814268103288 1104 7 0.022914611163997733 1104 8 -0.02558985549302406 1104 9 -0.2814569667669389 1104 10-11 0.13900237642131685 1104 12-13 0.02786216617671755 1104 14-15 0.29030104678248847 1104 16-17 0.09709400041634098 1104 18-19 -0.00957962518343436 1104 20-21 -0.19958800203551874 1104 22-23 0.13206384998643728 1104 24-25 -0.16081219335649877 1104 26-27 0.13752766538239314 1104 28-29 -0.019944329354451895 1104 30-31 0.1618358411300278 1104 32-33 0.4527402779642884 1104 34-35 0.5479317349527904 1104 36-37 0.49179867306964553 1104 38-39 0.46391097957546634 1104 40-41 0.23917103012708196 1104 42-43 0.08023440477192167 1104 44-45 0.7443897642801574 1104 46-47 1.1797577894323084 1104 48-49 1.957001013372782 1104 50-51 1.312621241841427 1104 52-53 0.6564660443282477 1104 54-55 1.04108325076227 1104 56-57 1.3472430559518855 1104 58-59 1.5626763254982379 1104 60-61 1.2465563148427208 1104 62-63 1.393653921281519 1104 64-65 1.2991877984692124 1104 66-67 1.1720540959335821 1104 68-69 1.283557664983494 1104 70-71 1.4875924302873074 1104 72-73 1.6895274253373742 1104 74-75 1.7820867301574417 1104 76-77 1.254160224420172 1104 78-79 1.437467616238287 1104 80-81 1.5611403683475515 1104 82-83 1.8563812056110685 1104 84-85 1.6743363213278748 1104 86-87 2.0668084419052555 1104 88-89 2.293157212816947 1104 90-91 2.7260796504815055 1104 92-93 2.782502287144464 1104 94-95 3.3381137198758815 1104 96-97 2.9666967778652484 1104 98-99 1.9364495523006973 1104 100-101 2.0281795112389247 1105 1 0.11670397036019153 1105 2 0.16391899968879642 1105 3 0.4335586665469222 1105 4 0.23564115196754187 1105 5 0.22800897006627707 1105 6 0.24760806628683696 1105 7 0.33857542094245474 1105 8 0.2342420772800864 1105 9 0.24975770091831606 1105 10-11 0.2540520326474507 1105 12-13 0.26252985143263174 1105 14-15 0.32917041271067404 1105 16-17 0.03993586443466768 1105 18-19 0.28369768574093257 1105 20-21 0.07524775044728926 1105 22-23 0.2101845528130255 1105 24-25 0.39428329938606055 1105 26-27 0.6200028372693325 1105 28-29 0.4107508578113297 1105 30-31 0.1846471474707414 1105 32-33 0.6181489869024119 1105 34-35 0.32447871738212086 1105 36-37 -0.20936251867978228 1105 38-39 0.3269820261759193 1105 40-41 0.05411373448154677 1105 42-43 0.3571175216550344 1105 44-45 0.03539052822210209 1105 46-47 -0.19500156885646192 1105 48-49 -0.02063076661193719 1105 50-51 0.3514294924143826 1105 52-53 0.6086738365360347 1105 54-55 0.5650251911748043 1105 56-57 0.07367544709016016 1105 58-59 0.05398266621634917 1105 60-61 0.18696884349053988 1105 62-63 -0.014153565349488417 1105 64-65 0.4819685471323183 1105 66-67 0.21753919906574026 1105 68-69 0.10007408973520882 1105 70-71 -0.10360695855913704 1105 72-73 0.396005652992077 1105 74-75 0.32706763160893004 1105 76-77 0.6720670235034412 1105 78-79 0.4675058133353076 1105 80-81 0.7923550360328093 1105 82-83 0.36900152646668616 1105 84-85 0.8414104236960931 1105 86-87 0.46738903777996654 1105 88-89 0.5192076329850188 1105 90-91 1.1968817895189403 1105 92-93 1.0905542351964144 1105 94-95 1.4587554311058284 1105 96-97 1.373663928361811 1105 98-99 2.238237176441263 1105 100-101 2.589310145310737 1106 1 0.2949182560744745 1106 2 -0.34125957173977284 1106 3 0.07177295226120606 1106 4 0.3479625805389759 1106 5 0.19247325578056262 1106 6 0.39350092342969845 1106 7 0.15286113522816436 1106 8 0.456027791565802 1106 9 0.16957912948974752 1106 10-11 -0.19193011020969664 1106 12-13 -0.020416577138789194 1106 14-15 0.2765811269963834 1106 16-17 0.5619001501489507 1106 18-19 0.7176262571695133 1106 20-21 0.22676560759015274 1106 22-23 0.2407202670987374 1106 24-25 0.07008687081462739 1106 26-27 0.010806408697902725 1106 28-29 0.37512585781132657 1106 30-31 0.2750042903278853 1106 32-33 0.4832382726166955 1106 34-35 -0.043378425475019355 1106 36-37 0.017066052748788252 1106 38-39 0.5191248833187743 1106 40-41 0.7056315916244102 1106 42-43 0.7163139502264713 1106 44-45 1.2183369567935287 1106 46-47 0.9512484311435401 1106 48-49 0.8901728048166291 1106 50-51 1.2050009209858104 1106 52-53 1.3138524079646103 1106 54-55 1.2710966197462312 1106 56-57 1.0982290185187296 1106 58-59 1.0155005233592007 1106 60-61 1.5717902720619676 1106 62-63 1.7439714346505113 1106 64-65 1.7013435471323177 1106 66-67 1.975396341922881 1106 68-69 1.9562348040209265 1106 70-71 1.1842501842980084 1106 72-73 1.221094938706365 1106 74-75 1.3677819173232173 1106 76-77 1.970102737789155 1106 78-79 2.0254522419067342 1106 80-81 1.4621764646042372 1106 82-83 1.010876526466685 1106 84-85 0.9503389951246639 1106 86-87 1.0885497520656813 1106 88-89 2.1861719186993014 1106 90-91 2.0737567895189386 1106 92-93 2.289036378053556 1106 94-95 1.5693804311058308 1106 96-97 1.2932174997903836 1106 98-99 1.4510050335841207 1106 100-101 0.7200244310250206 1107 1 0.17490380520742121 1107 2 0.276189850225542 1107 3 0.15002439734791295 1107 4 0.10190044181064906 1107 5 0.06563077023142938 1107 6 0.07446190608865777 1107 7 0.21817905430331308 1107 8 0.12979946786637697 1107 9 -0.003289367620077144 1107 10-11 0.22275558343191904 1107 12-13 0.03650177546236222 1107 14-15 0.0818376298865644 1107 16-17 0.45882934090040095 1107 18-19 0.4054152745105455 1107 20-21 0.7577085266652972 1107 22-23 0.6544124636305355 1107 24-25 0.36663672630596267 1107 26-27 0.293627940489813 1107 28-29 0.6699416092564121 1107 30-31 0.8886603596920537 1107 32-33 0.6874470876456016 1107 34-35 0.8492516323284462 1107 36-37 1.2524706770262526 1107 38-39 1.1799630336077982 1107 40-41 1.1729906956706486 1107 42-43 1.0678854820183759 1107 44-45 1.1359850781808092 1107 46-47 1.2056920727620408 1107 48-49 0.9631677470131592 1107 50-51 1.34878704815344 1107 52-53 1.5229925813750143 1107 54-55 1.5688567353531653 1107 56-57 1.3630230936632444 1107 58-59 1.7022237892551573 1107 60-61 1.577968017726711 1107 62-63 1.6999504808932855 1107 64-65 1.5385003390398353 1107 66-67 1.5807431627321265 1107 68-69 1.6551690525758325 1107 70-71 1.2552039415234333 1107 72-73 1.6981361236774575 1107 74-75 1.5557876976700378 1107 76-77 1.595138864956784 1107 78-79 1.6415107679182945 1107 80-81 1.4115623027545254 1107 82-83 1.3491171334031016 1107 84-85 2.107058648303859 1107 86-87 1.3757787983084562 1107 88-89 1.5690837684102839 1107 90-91 1.1843962403859933 1107 92-93 1.0497119560882382 1107 94-95 0.6307785524931155 1107 96-97 0.7668266038366234 1107 98-99 0.9544551780927932 1107 100-101 0.1065851246666405 1108 1 0.3922774110040521 1108 2 0.1426312733306503 1108 3 0.20575182550064852 1108 4 0.043649200257284804 1108 5 -0.007966885064512041 1108 6 -0.07102372445762484 1108 7 -0.1274205549126819 1108 8 0.2474010310024184 1108 9 0.025653073151715944 1108 10-11 0.04922305880438671 1108 12-13 0.13085983131190915 1108 14-15 0.25866739460202126 1108 16-17 0.32677691071233284 1108 18-19 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-0.4288904074547517 2316 64-65 -0.22570062580001604 2316 66-67 -0.24095704153576136 2316 68-69 -0.18819658695651853 2316 70-71 -0.11819342472455219 2316 72-73 -0.3999341966319818 2316 74-75 -0.12451131575949148 2316 76-77 0.1947737904207365 2316 78-79 -0.5651257656120627 2316 80-81 -0.327344212087489 2316 82-83 -0.8621262930821878 2316 84-85 -0.12024371164224945 2316 86-87 0.1416747520656827 2316 88-89 0.4192076329850174 2316 90-91 0.05131787974450219 2316 92-93 -0.03636305803666673 2316 94-95 0.353191521331393 2316 96-97 0.5267466351287275 2316 98-99 0.896282289223219 2316 100-101 2.200287588919757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 863.0 3 115.0 4 78.0 5 94.0 6 96.0 7 102.0 8 149.0 9 161.0 10 218.0 11 369.0 12 600.0 13 897.0 14 940.0 15 1589.0 16 2191.0 17 3185.0 18 2575.0 19 1358.0 20 1383.0 21 1338.0 22 1426.0 23 1524.0 24 1702.0 25 1840.0 26 2048.0 27 2481.0 28 2841.0 29 3355.0 30 4085.0 31 5129.0 32 6579.0 33 8784.0 34 12134.0 35 17403.0 36 24926.0 37 35397.0 38 26869.0 39 2568.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.49689649387686 24.46289772409551 16.656042051109992 20.38416373091763 2 14.038480888123694 23.87111921025859 40.07289232729996 22.01750757431775 3 16.735052099637763 28.75374536022539 34.51824605339654 19.992956486740308 4 10.3790038571189 20.69092738554419 42.62116384370284 26.30890491363408 5 9.449087402521172 40.50255750901417 37.94001732956927 12.108337758895379 6 22.87221557823205 43.022878921661764 19.482290351063472 14.622615149042705 7 21.13642586911208 37.50048897159494 25.059375122242898 16.303710037050077 8 21.942918457334144 35.20736348547254 22.892973504641297 19.956744552552017 9 23.16953207091596 18.56122426166469 25.72548011357285 32.5437635538465 10-11 20.55386008451271 28.951192791825964 30.790685715563303 19.704261408098017 12-13 22.392752047708605 29.02282870229349 30.092862991457054 18.491556258540854 14-15 20.442765453900254 30.277992774255562 29.71692182169232 19.562319950151863 16-17 16.492175123010252 32.94439461632816 31.167905850007127 19.395524410654463 18-19 16.089812178897994 31.93042545404767 34.64423504038307 17.335527326671265 20-21 16.978269736878865 30.460922963451363 34.34012974458979 18.220677555079988 22-23 17.20773778259558 30.1367298816535 34.07965758859225 18.57587474715868 24-25 17.604970762684637 31.03960797132424 33.937915755183234 17.41750551080789 26-27 17.067296946940235 30.272171561396448 35.33871977113227 17.321811720531045 28-29 16.13884790772532 30.7255342453505 35.50943311874106 17.62618472818312 30-31 16.937458081824282 30.162921976302258 34.78649675832774 18.113123183545717 32-33 16.54320642682501 31.075375480265453 34.66433810688089 17.71707998602864 34-35 17.63930065357651 30.591236653934175 34.03767216110563 17.731790531383687 36-37 17.733787823754575 29.834595289318543 34.484646978290634 17.946969908636248 38-39 16.61777770328031 31.499578158087356 33.950172368516625 17.93247177011571 40-41 16.230909126464205 31.319788020415746 34.964618852997134 17.48468400012292 42-43 16.487673498580723 31.13894414519121 33.48857870856708 18.884803647660984 44-45 16.924955229051246 31.06028223159083 33.252312583639956 18.76244995571797 46-47 17.772680026376673 31.230930001229424 32.70064712259565 18.295742849798263 48-49 17.298987392703864 31.327677932761087 32.60405489985694 18.76927977467811 50-51 16.702716902250764 31.447822174527857 33.01642661122196 18.83303431199942 52-53 17.11632144843527 32.19543143661279 32.099845441358546 18.588401673593392 54-55 17.51087688511718 30.981448518871428 32.074027088124424 19.433647507886963 56-57 17.75544014236983 31.696285141964736 31.39707381942834 19.15120089623709 58-59 17.9320651414844 30.4156540685494 32.157322830246656 19.494957959719546 60-61 17.845739554554875 31.066068121314377 32.547439933627395 18.540752390503354 62-63 17.306026896709707 30.7029796458842 32.91578435699879 19.075209100407307 64-65 17.20388957017512 31.07270456977007 32.57892134542702 19.14448451462779 66-67 17.20251979942173 30.746302012766613 31.69723296970374 20.353945218107917 68-69 16.805039528452102 30.094421320222363 32.72760287174903 20.3729362795765 70-71 17.514806123589228 30.324896636495698 32.80981115208404 19.350486087831044 72-73 17.543413642052567 29.177375737529054 33.28267477203647 19.996535848381907 74-75 16.493840265944073 31.12411654607928 32.22756096879627 20.154482219180377 76-77 16.93778219857839 29.92841655863025 32.76576959184585 20.368031650945507 78-79 16.80771584155926 29.784891859194833 32.70906616971163 20.698326129534273 80-81 17.25444289541839 29.651525014597784 32.097806560482326 20.996225529501498 82-83 16.931410564426912 29.85171361843355 32.618254757567975 20.598621059571567 84-85 16.776765089535857 29.671268920402333 32.097158483071844 21.45480750698997 86-87 16.742272303197225 29.97335180643467 32.21414644774552 21.070229442622583 88-89 17.573045258867538 28.761352430279107 31.61388188164699 22.05172042920637 90-91 16.989038248801585 27.748259640418805 32.87407953694703 22.38862257383259 92-93 17.298747014206093 28.04928131416838 33.15090307170096 21.50106859992457 94-95 17.01278892408776 27.358743567824877 33.03526033198684 22.593207176100524 96-97 16.895655817851654 27.264422405363874 32.65092890068445 23.188992876100016 98-99 16.72054110987872 28.167440093202323 31.82037878809044 23.29164000882852 100-101 16.84591618385693 27.399755289490297 32.843167380871236 22.911161145781538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 474.0 1 297.0 2 136.0 3 163.0 4 139.0 5 128.5 6 211.0 7 390.0 8 598.0 9 688.5 10 584.0 11 431.0 12 385.5 13 367.5 14 368.0 15 411.0 16 448.5 17 450.0 18 456.0 19 500.5 20 599.0 21 673.5 22 743.0 23 882.0 24 1008.0 25 1268.5 26 1914.5 27 2738.5 28 3015.0 29 3260.5 30 4049.5 31 4807.0 32 5763.0 33 7219.5 34 8977.5 35 9899.5 36 10732.5 37 11729.0 38 11024.5 39 9576.5 40 8375.5 41 7444.0 42 6624.0 43 6042.0 44 5450.0 45 4957.0 46 4720.5 47 4920.5 48 6644.5 49 6245.0 50 3499.0 51 1830.5 52 1065.0 53 779.0 54 613.5 55 494.5 56 415.5 57 346.0 58 293.5 59 284.0 60 233.5 61 195.0 62 165.0 63 123.5 64 95.0 65 66.5 66 43.0 67 33.5 68 26.5 69 19.0 70 13.5 71 12.0 72 13.5 73 10.0 74 6.5 75 6.0 76 4.0 77 3.0 78 2.5 79 1.5 80 1.0 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3132804138423118 2 0.2776043524794863 3 0.2809489832322512 4 0.2798341063146629 5 0.2826212986086336 6 0.25084730645736714 7 0.24806011416339635 8 0.25363449875133787 9 0.265340706386015 10-11 0.26980021405636817 12-13 0.2633896717802355 14-15 0.25001114876917585 16-17 0.24722395647520512 18-19 0.2332879950053514 20-21 0.23691134498751337 22-23 0.23746878344630754 24-25 0.23719006421691044 26-27 0.23635390652871924 28-29 0.2497324295397788 30-31 0.2631109525508384 32-33 0.25335577952194077 34-35 0.25279834106314664 36-37 0.2436006064930432 38-39 0.23133696039957188 40-41 0.2299433642525865 42-43 0.23858366036389583 44-45 0.23719006421691044 46-47 0.2486175526221905 48-49 0.2497324295397788 50-51 0.26478326792722084 52-53 0.27621075633250086 54-55 0.25502809489832323 56-57 0.23496031038173384 58-59 0.2213030681412772 60-61 0.22436897966464503 62-63 0.2291072065643953 64-65 0.22325410274705673 66-67 0.22827104887620406 68-69 0.22687745272921866 70-71 0.22966464502318945 72-73 0.23189439885836602 74-75 0.22687745272921866 76-77 0.24527292186942562 78-79 0.24276444880485196 80-81 0.23774750267570463 82-83 0.23077952194077772 84-85 0.21489252586514448 86-87 0.2190733143061006 88-89 0.2288284873349982 90-91 0.22632001427042456 92-93 0.23384543346414555 94-95 0.227992329646807 96-97 0.23245183731716018 98-99 0.23774750267570463 100-101 0.22576257581163037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179392.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.507491972886193 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24221605028505 22.344363182304676 2 9.631267358573307 5.87651623260792 3 4.251936851337524 3.891477880841955 4 2.3625931881303903 2.8830717088833393 5 1.6901768747259172 2.5781528719229394 6 1.1950007308872972 2.187388512308241 7 0.9227452126882035 1.970544951837317 8 0.6577985674608975 1.6054227613271492 9 0.6011548019295425 1.6505752764894757 >10 4.728840812746674 28.81789600428113 >50 0.4823856161379915 10.038351765965038 >100 0.22109340739657943 11.913017302889761 >500 0.00730887297178775 1.8044282911166605 >1k 0.005481654728840813 2.438793257224402 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1733 0.9660408490902603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1594 0.8885569033178737 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1048 0.5841955048162683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 0.5479620049946486 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 961 0.5356983589011773 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 659 0.3673519443453443 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 634 0.3534159828754906 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 457 0.2547493756689262 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.23189439885836602 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 378 0.21071173742418836 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 368 0.20513735283624687 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 366 0.20402247591865857 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 354 0.1973332144131288 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 352 0.1962183374955405 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 350 0.1951034605779522 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 335 0.18674188369603997 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 326 0.18172493756689262 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 312 0.17392079914377453 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 303 0.16890385301462718 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 301 0.16778897609703888 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.16778897609703888 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 296 0.16500178380306815 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 295 0.164444345344274 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 284 0.15831252229753834 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 279 0.1555253300035676 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 278 0.15496789154477347 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 267 0.14883606849803782 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 267 0.14883606849803782 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 266 0.14827863003924366 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 262 0.14604887620406706 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 243 0.13545754548697825 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 240 0.1337852301105958 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 222 0.12375133785230111 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 217 0.12096414555833035 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 214 0.11929183018194792 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 211 0.11761951480556547 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 208 0.11594719942918301 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 206 0.11483232251159473 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 199 0.11093025330003567 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 195 0.10870049946485909 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 194 0.10814306100606494 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 193 0.10758562254727078 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 191 0.10647074562968248 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 190 0.10591330717088833 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 190 0.10591330717088833 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 183 0.10201123795932929 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 183 0.10201123795932929 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 183 0.10201123795932929 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 182 0.10145379950053514 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.361576881912237E-4 0.0 24-25 0.0 0.0 0.0 0.00306591152336782 0.0 26-27 0.0 0.0 0.0 0.005853103817338566 0.0 28-29 0.0 0.0 0.0 0.018674188369603995 0.0 30-31 0.0 0.0 0.0 0.040135569033178733 0.0 32-33 0.0 0.0 0.0 0.07163084195504817 0.0 34-35 0.0 0.0 0.0 0.10730690331787371 0.0 36-37 0.0 0.0 0.0 0.14409784159828753 0.0 38-39 0.0 0.0 0.0 0.17252720299678914 0.0 40-41 0.0 0.0 0.0 0.20235016054227611 0.0 42-43 0.0 0.0 0.0 0.23468159115233678 0.0 44-45 0.0 0.0 0.0 0.2619960756332501 0.0 46-47 0.0 0.0 0.0 0.30519755618979666 0.0 48-49 0.0 0.0 0.0 0.3581542097752408 0.0 50-51 0.0 0.0 0.0 0.39187923653228685 0.0 52-53 0.0 0.0 0.0 0.452082590082055 0.0 54-55 0.0 0.0 0.0 0.6326926507313593 0.0 56-57 0.0 0.0 0.0 0.9983722797003212 0.0 58-59 0.0 0.0 0.0 1.338131020335355 0.0 60-61 0.0 0.0 0.0 1.6148992151266501 0.0 62-63 0.0 0.0 0.0 1.9950722440242596 0.0 64-65 0.0 0.0 0.0 2.4574674455940064 0.0 66-67 0.0 0.0 0.0 3.1311318230467355 0.0 68-69 0.0 0.0 0.0 3.913217980734927 0.0 70-71 0.0 0.0 0.0 4.681646896182661 0.0 72-73 0.0 0.0 0.0 5.370919550481627 0.0 74-75 0.0 0.0 0.0 6.221013200142703 0.0 76-77 0.0 0.0 0.0 7.218270602925437 0.0 78-79 0.0 0.0 0.0 8.044673118087763 0.0 80-81 0.0 0.0 0.0 8.639181234391723 0.0 82-83 0.0 0.0 0.0 9.326781573314307 0.0 84-85 0.0 0.0 0.0 10.327104887620408 0.0 86-87 0.0 0.0 0.0 11.549567427755974 0.0 88-89 0.0 0.0 0.0 13.124609793078843 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTCG 30 1.2988377E-4 63.327366 4 TAGTCGA 30 1.2988377E-4 63.327366 5 GTCGAAT 40 5.378394E-4 47.49552 7 AGTCGAA 40 5.378394E-4 47.49552 6 GGTACTC 45 9.607683E-4 42.21824 3 CCTAGTC 45 9.607683E-4 42.21824 3 CATATAG 50 0.0016129115 37.996418 3 TACTCCC 50 0.0016129115 37.996418 5 CAACCGT 90 5.6663157E-7 36.94096 6 ACAACCG 95 8.651732E-7 34.9967 5 AACCGTC 95 8.651732E-7 34.9967 7 GTGGTAC 55 0.0025714776 34.55187 1 ACCGTCT 100 1.2920191E-6 33.246864 8 CCCTATA 90 2.3986071E-5 31.672544 2 GTTATTC 75 3.0698234E-4 31.663683 3 CCGTCTC 95 3.4767734E-5 29.997171 9 GGTTATT 80 4.473759E-4 29.69301 2 ACTTAGA 65 0.005833728 29.228014 7 CTTAGAT 65 0.005833728 29.228014 8 TAGATCA 85 6.389375E-4 27.938545 5 >>END_MODULE