##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937315_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179392 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32936808776311 33.0 31.0 34.0 31.0 34.0 2 32.53684110774171 34.0 31.0 34.0 31.0 34.0 3 32.5550581965751 34.0 31.0 34.0 31.0 34.0 4 36.18510301462719 37.0 35.0 37.0 35.0 37.0 5 36.13934289154477 37.0 35.0 37.0 35.0 37.0 6 36.217551507313594 37.0 36.0 37.0 35.0 37.0 7 36.170793569389936 37.0 36.0 37.0 35.0 37.0 8 36.16404856403853 37.0 36.0 37.0 35.0 37.0 9 37.85658223332144 39.0 38.0 39.0 35.0 39.0 10-11 37.80561563503389 39.0 37.5 39.0 35.0 39.0 12-13 37.74461793168034 39.0 37.0 39.0 35.0 39.0 14-15 38.9180398234035 40.0 38.0 41.0 36.0 41.0 16-17 38.86555141812344 40.0 38.0 41.0 35.5 41.0 18-19 38.95966096592936 40.0 38.0 41.0 35.5 41.0 20-21 38.928079290046384 40.0 38.0 41.0 35.0 41.0 22-23 38.861738539065286 40.0 38.0 41.0 35.0 41.0 24-25 38.85208091776668 40.0 38.0 41.0 35.0 41.0 26-27 38.673527805030325 40.0 38.0 41.0 35.0 41.0 28-29 38.536230712629326 40.0 38.0 41.0 34.5 41.0 30-31 38.48750222975384 40.0 38.0 41.0 35.0 41.0 32-33 38.26216051997859 40.0 37.0 41.0 34.0 41.0 34-35 38.08251482786301 40.0 37.0 41.0 34.0 41.0 36-37 37.87142403228684 40.0 37.0 41.0 33.5 41.0 38-39 37.69062165536924 40.0 37.0 41.0 33.0 41.0 40-41 37.500039020692114 40.0 37.0 41.0 33.0 41.0 42-43 37.22191067606136 40.0 36.0 41.0 33.0 41.0 44-45 36.78322054495184 40.0 36.0 41.0 32.5 41.0 46-47 36.22768295130218 40.0 36.0 41.0 30.5 41.0 48-49 35.6819980824117 40.0 36.0 41.0 28.5 41.0 50-51 35.37826101498395 39.0 35.0 41.0 26.5 41.0 52-53 35.179361398501605 39.0 35.0 41.0 26.0 41.0 54-55 34.976236398501605 39.0 35.0 40.5 24.0 41.0 56-57 34.69513133250089 39.0 35.0 40.0 23.0 41.0 58-59 34.44709351587585 39.0 35.0 40.0 22.0 41.0 60-61 34.07805810738495 38.0 34.5 40.0 18.5 41.0 62-63 33.72957545486978 38.0 34.0 40.0 14.0 41.0 64-65 33.34316468961826 38.0 34.0 40.0 8.0 41.0 66-67 32.88246967534784 37.0 33.0 39.5 4.0 41.0 68-69 32.393077729218696 36.5 33.0 39.0 2.0 40.0 70-71 31.95061931412772 36.0 32.0 39.0 2.0 40.0 72-73 31.468780659115232 35.5 31.5 38.0 2.0 40.0 74-75 30.953127787192294 35.0 31.0 37.0 2.0 39.5 76-77 29.60477891990724 34.0 29.5 36.0 2.0 38.5 78-79 30.165955003567607 34.5 31.0 36.0 2.0 39.0 80-81 29.914023479307886 34.5 31.0 36.0 2.0 38.0 82-83 29.510265229218696 34.0 30.5 35.5 2.0 37.0 84-85 29.057212696218336 34.0 29.5 35.0 2.0 37.0 86-87 28.744227724759185 34.0 29.0 35.0 2.0 36.0 88-89 28.283212183374957 34.0 29.0 35.0 2.0 36.0 90-91 27.778147854976808 34.0 28.5 35.0 2.0 35.5 92-93 27.394033736175526 34.0 27.0 35.0 2.0 35.0 94-95 26.929447801462718 33.0 26.0 35.0 2.0 35.0 96-97 26.293956809668213 33.0 25.0 35.0 2.0 35.0 98-99 25.471448002140562 32.5 22.0 34.5 2.0 35.0 100-101 22.682864899215126 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31522497839767283 1101 2 0.22278592620398285 1101 3 0.19964148580627494 1101 4 0.05598520475255242 1101 5 0.10736082072546793 1101 6 0.24657152481569256 1101 7 0.173951884429016 1101 8 0.31247988424637185 1101 9 0.3192252385707093 1101 10-11 0.33860065693114905 1101 12-13 0.3532094887983064 1101 14-15 0.6359145882624873 1101 16-17 0.3738414156200207 1101 18-19 0.2690219766093733 1101 20-21 0.37236673709812607 1101 22-23 0.2067956695138804 1101 24-25 0.19917002060879696 1101 26-27 0.2859600681457408 1101 28-29 0.23627836543554537 1101 30-31 0.22835178096360664 1101 32-33 0.49386529448060656 1101 34-35 0.5559020175860141 1101 36-37 0.5705660356965794 1101 38-39 0.24142976557521934 1101 40-41 0.437788009833902 1101 42-43 0.5244048866873925 1101 44-45 0.6476097003536907 1101 46-47 0.6476708105657067 1101 48-49 0.9972399690867562 1101 50-51 1.2900120124273187 1101 52-53 0.9236185664895729 1101 54-55 1.25855290778631 1101 56-57 0.8850309505683711 1101 58-59 0.9618131593905019 1101 60-61 0.4060727765248586 1101 62-63 0.42350245883515925 1101 64-65 0.3016032701324036 1101 66-67 0.2844399833000253 1101 68-69 0.4804269202266518 1101 70-71 0.3229264352677603 1101 72-73 0.190529356707291 1101 74-75 -0.3156615877054172 1101 76-77 -0.4218954523307836 1101 78-79 -0.2147080986070904 1101 80-81 0.07338687205820804 1101 82-83 -0.2581531093907685 1101 84-85 -0.19539897154828267 1101 86-87 0.03670717224384745 1101 88-89 0.42945502316356965 1101 90-91 0.631457753288629 1101 92-93 0.6507032871048075 1101 94-95 0.7697582120778783 1101 96-97 0.6654984005859816 1101 98-99 1.6991421208780508 1101 100-101 1.9830768598070527 1102 1 0.1868548803584531 1102 2 0.1344949279865162 1102 3 0.036344917178823266 1102 4 0.13656118514471416 1102 5 -0.06902621136009657 1102 6 0.10853676545740143 1102 7 0.1343184174058436 1102 8 0.16993309280252333 1102 9 0.21561561290226194 1102 10-11 0.26158973892758297 1102 12-13 0.12493408772878922 1102 14-15 0.40905964173842335 1102 16-17 0.30124504752732406 1102 18-19 0.2017035719747824 1102 20-21 0.09885960697335605 1102 22-23 0.22127873546752852 1102 24-25 0.13809047515425021 1102 26-27 0.01458361983914358 1102 28-29 0.24056758112181598 1102 30-31 0.20607014103489973 1102 32-33 0.01738635063033911 1102 34-35 0.35408606393182396 1102 36-37 0.6355587941636003 1102 38-39 0.17773212742905287 1102 40-41 0.23540108915653235 1102 42-43 0.2060559562061144 1102 44-45 -0.11610743422742331 1102 46-47 -0.05080289709916741 1102 48-49 -0.28389862271360045 1102 50-51 -0.3998414857901551 1102 52-53 -0.4335421955425147 1102 54-55 -0.423167791857189 1102 56-57 -0.21881541752431843 1102 58-59 -0.2087527942636811 1102 60-61 -0.011345895489405677 1102 62-63 0.1051256763039703 1102 64-65 0.19091922378659376 1102 66-67 0.09805406529645921 1102 68-69 0.21279657263307072 1102 70-71 -0.3015722278338444 1102 72-73 -0.19941326806989323 1102 74-75 -0.06451965365907242 1102 76-77 -0.09702914217035641 1102 78-79 0.12239918748916523 1102 80-81 0.5567591982614175 1102 82-83 0.11720896725807606 1102 84-85 0.09288367105421003 1102 86-87 0.4105791642224581 1102 88-89 0.5394149162116975 1102 90-91 0.41859846184478045 1102 92-93 0.6428350830049894 1102 94-95 0.6399119553933872 1102 96-97 0.1069951697481919 1102 98-99 1.0719696609850047 1102 100-101 1.8962202421599947 1103 1 0.2591237879214816 1103 2 0.29058354525160723 1103 3 0.15264743818722337 1103 4 0.26530068094302806 1103 5 0.09280724929689654 1103 6 0.10621438195855148 1103 7 0.2333864082385375 1103 8 0.19801559853208772 1103 9 0.4913680957135682 1103 10-11 0.30738041883590483 1103 12-13 0.12499520308401912 1103 14-15 0.19743244540534732 1103 16-17 0.09180272514382892 1103 18-19 0.5838731670855566 1103 20-21 0.4901048323362218 1103 22-23 0.47370043141863505 1103 24-25 0.30925930632308507 1103 26-27 0.5240106633838337 1103 28-29 0.5672902701974465 1103 30-31 0.3902565428683644 1103 32-33 0.4427492230520329 1103 34-35 0.8181043985384022 1103 36-37 0.874122583315625 1103 38-39 0.4122928608133236 1103 40-41 0.7299159860243734 1103 42-43 1.1620834581159585 1103 44-45 1.1906305336870204 1103 46-47 1.4477303343752368 1103 48-49 1.24896615956294 1103 50-51 0.699104274332079 1103 52-53 0.6128596379181417 1103 54-55 0.33204993159583296 1103 56-57 0.41459940294932807 1103 58-59 0.28955125462860565 1103 60-61 0.6889596812867538 1103 62-63 0.9593655540732584 1103 64-65 0.6211866034657376 1103 66-67 0.7324161737762083 1103 68-69 1.0840876345123682 1103 70-71 1.0306050066963337 1103 72-73 1.0400978090882411 1103 74-75 1.1316300789612477 1103 76-77 1.2566759762406434 1103 78-79 0.9717651156786218 1103 80-81 1.3209761577724919 1103 82-83 1.4833052239425655 1103 84-85 1.5999730522612374 1103 86-87 1.3596089579581339 1103 88-89 1.168383594592143 1103 90-91 1.760966681860058 1103 92-93 1.8021169775810009 1103 94-95 1.7460082120778786 1103 96-97 1.1278049482050285 1103 98-99 1.1296778351637649 1103 100-101 1.6600857883784812 1104 1 0.10129338303224955 1104 2 0.1612328959009517 1104 3 0.13794919525368954 1104 4 -0.07734255817079827 1104 5 0.05396844104632237 1104 6 -0.15349532010409206 1104 7 0.03271413933097733 1104 8 -0.0707086184274246 1104 9 -0.08384962773945404 1104 10-11 -0.06728726642000993 1104 12-13 0.1088913069801194 1104 14-15 0.11494199467959731 1104 16-17 -0.22549292038711144 1104 18-19 0.0840565131512605 1104 20-21 0.04270987435302942 1104 22-23 0.12502205097554508 1104 24-25 0.03381240028794252 1104 26-27 -0.030870925615403166 1104 28-29 -0.12841637609744083 1104 30-31 -0.13350205147846594 1104 32-33 0.009364960256000643 1104 34-35 -0.30634174355480326 1104 36-37 -0.3590936122535098 1104 38-39 -0.4532839153516903 1104 40-41 -0.973155060576083 1104 42-43 -1.189665968927578 1104 44-45 -0.8754657229974683 1104 46-47 -0.8903750896125402 1104 48-49 -1.257160654799172 1104 50-51 -0.8971676889987137 1104 52-53 -0.7918309655959916 1104 54-55 -0.30552073303366 1104 56-57 -0.5263020485403587 1104 58-59 0.02119372980048695 1104 60-61 0.25870758044641917 1104 62-63 0.16394920571573834 1104 64-65 -0.13795778156099203 1104 66-67 -0.08911171010461061 1104 68-69 0.1700158239699654 1104 70-71 0.17971119462605145 1104 72-73 0.07331400465738014 1104 74-75 0.32050708430884 1104 76-77 0.06339866531627436 1104 78-79 -0.23321578577286317 1104 80-81 0.05408540146997254 1104 82-83 -0.04054504343711329 1104 84-85 -0.12636766584418524 1104 86-87 0.17528504657540012 1104 88-89 0.5180245418801448 1104 90-91 0.5362455206683094 1104 92-93 0.4182361525237077 1104 94-95 0.212104468762373 1104 96-97 0.32624650664658716 1104 98-99 1.1548573615197597 1104 100-101 1.4523699747803143 1105 1 -0.043733354935660884 1105 2 0.11915497382302931 1105 3 0.13550458104436558 1105 4 0.008157823800175379 1105 5 0.20137867786832686 1105 6 -0.1509284751843083 1105 7 0.2333864082385375 1105 8 0.19801559853208772 1105 9 0.27993952428499824 1105 10-11 0.3416661331216204 1105 12-13 0.07642377451259108 1105 14-15 0.45743244540534533 1105 16-17 0.22894558228668416 1105 18-19 0.3610160242284124 1105 20-21 0.33296197519337056 1105 22-23 0.5822718599900654 1105 24-25 0.39783073489451226 1105 26-27 0.16686780624097963 1105 28-29 0.47300455591173574 1105 30-31 0.2016851142969358 1105 32-33 0.13132065162346152 1105 34-35 0.22096154139554613 1105 36-37 0.3855511547441921 1105 38-39 0.24943571795617459 1105 40-41 0.27563027173865606 1105 42-43 -0.05505939902689505 1105 44-45 -0.4293694663129841 1105 46-47 -0.8522696656247675 1105 48-49 -1.299605269008488 1105 50-51 -0.8637528685250686 1105 52-53 -0.6414260763675728 1105 54-55 -0.9336643541184557 1105 56-57 -0.8654005970506731 1105 58-59 -0.6647344596571116 1105 60-61 -0.4453260329989561 1105 62-63 -0.2920630173553178 1105 64-65 -0.3702419679628335 1105 66-67 -0.11044096908093337 1105 68-69 -0.21591236548763604 1105 70-71 -0.01510927901795256 1105 72-73 -0.2827593337689045 1105 74-75 -0.1283699210387539 1105 76-77 -0.15760973804507117 1105 78-79 -0.3396634557499496 1105 80-81 -0.3790238422275074 1105 82-83 -0.016694776057434524 1105 84-85 0.3228301951183816 1105 86-87 0.3281803865295636 1105 88-89 0.39409788030642545 1105 90-91 0.275252396145774 1105 92-93 0.6392598347238554 1105 94-95 0.47457964064930636 1105 96-97 0.8306620910621696 1105 98-99 0.3925349780209082 1105 100-101 1.6400857883784816 1106 1 0.047516645064334284 1106 2 0.1686192595373157 1106 3 0.06318315247293782 1106 4 -0.01380646191411472 1106 5 0.04798582072546509 1106 6 -0.0846784751843046 1106 7 0.06874355109567887 1106 8 0.21872988424637185 1106 9 0.019225238570712122 1106 10-11 0.0813089902644748 1106 12-13 -0.22491551120169362 1106 14-15 -0.026585411737514164 1106 16-17 0.07644558228668785 1106 18-19 0.20235530994270334 1106 20-21 -0.02346659623520253 1106 22-23 -0.08799599715278816 1106 24-25 0.2522950206087984 1106 26-27 0.3510642348124051 1106 28-29 0.016486698768872543 1106 30-31 0.13043511429692956 1106 32-33 0.3485527944806037 1106 34-35 0.21319368425267982 1106 36-37 0.5460868690299137 1106 38-39 0.5955964322418907 1106 40-41 0.3414338431672306 1106 42-43 0.06502988668739818 1106 44-45 0.2856305336870193 1106 46-47 0.358087477232381 1106 48-49 0.22744830242007907 1106 50-51 0.7936578457606487 1106 52-53 0.8064310664895729 1106 54-55 0.4955320744529743 1106 56-57 0.7282601172350454 1106 58-59 0.9316048260571748 1106 60-61 1.09565610985819 1106 62-63 1.158919125501825 1106 64-65 1.3099366034657365 1106 66-67 1.3740233166333553 1106 68-69 1.421051920226649 1106 70-71 1.301051435267759 1106 72-73 1.3962585233739553 1106 74-75 1.429130078961247 1106 76-77 1.353104547669215 1106 78-79 1.3649794013929082 1106 80-81 0.964011872058208 1106 82-83 1.7783052239425672 1106 84-85 2.00408019511838 1106 86-87 1.6976446722438467 1106 88-89 1.357580023163571 1106 90-91 1.0423952532886318 1106 92-93 0.9355991204381446 1106 94-95 0.522883212077879 1106 96-97 1.0378942339193138 1106 98-99 1.5241421208780501 1106 100-101 2.0377643598070527 1107 1 -0.05829982892409902 1107 2 0.1953894907511895 1107 3 0.08496783455386492 1107 4 -0.03038883185630681 1107 5 0.12388899991621827 1107 6 0.12699059996020168 1107 7 0.02965395571995799 1107 8 -0.017541792054203142 1107 9 0.2000055853915157 1107 10-11 0.0829708399754594 1107 12-13 -0.038770279987822676 1107 14-15 -0.021328908847337402 1107 16-17 0.10224037997454616 1107 18-19 0.3641869573415448 1107 20-21 0.21135999336016909 1107 22-23 -0.15279007229730013 1107 24-25 -0.0014260776570935718 1107 26-27 0.3845721827892845 1107 28-29 0.1463819299827449 1107 30-31 0.3911648830830643 1107 32-33 0.3604205690470792 1107 34-35 0.33329845303881456 1107 36-37 0.47105074186227824 1107 38-39 0.920831258831484 1107 40-41 0.6012784963464171 1107 42-43 1.0029092219475046 1107 44-45 0.9510929614326855 1107 46-47 0.5861944136485633 1107 48-49 0.7332105856570834 1107 50-51 1.0656773544311662 1107 52-53 1.2363443612872587 1107 54-55 1.123693201620604 1107 56-57 1.0185960998940047 1107 58-59 0.8456041035138213 1107 60-61 0.9013461387599264 1107 62-63 0.7510253393746638 1107 64-65 0.714597008090017 1107 66-67 0.7546952819512711 1107 68-69 1.0319984520185592 1107 70-71 0.7624134294874096 1107 72-73 0.6651349684606593 1107 74-75 0.9396069575739645 1107 76-77 0.7298386517154576 1107 78-79 0.8786354707570716 1107 80-81 0.5530653402663006 1107 82-83 0.5853861487980581 1107 84-85 0.6783215245981502 1107 86-87 0.6510044988334407 1107 88-89 0.9522151387705051 1107 90-91 1.1328576810342952 1107 92-93 0.9189625597445037 1107 94-95 1.4173376918466651 1107 96-97 2.2413805055956146 1107 98-99 2.2051031035370094 1107 100-101 1.9060808337954924 1108 1 0.06133706759954549 1108 2 0.13050306235422227 1108 3 -0.19729219963974032 1108 4 -0.14347195487185616 1108 5 -0.08872192575340421 1108 6 -0.021914390677267193 1108 7 0.004130875039344062 1108 8 -0.22669265096489255 1108 9 -0.13676067692225047 1108 10-11 -0.10513467170735424 1108 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-0.8611429217411164 2316 88-89 -0.7815412174379333 2316 90-91 -0.5354242955835531 2316 92-93 -0.6565296389603539 2316 94-95 -1.006322614989788 2316 96-97 -1.1194130969077527 2316 98-99 -1.5619011122046587 2316 100-101 0.13527375830329547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 0.0 6 5.0 7 17.0 8 56.0 9 94.0 10 140.0 11 295.0 12 486.0 13 794.0 14 1007.0 15 1565.0 16 2256.0 17 3522.0 18 3060.0 19 1486.0 20 1145.0 21 1113.0 22 1078.0 23 1140.0 24 1247.0 25 1421.0 26 1533.0 27 1726.0 28 2165.0 29 2627.0 30 3203.0 31 4129.0 32 5160.0 33 7271.0 34 10107.0 35 15474.0 36 24777.0 37 41611.0 38 34709.0 39 2971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.85243762338691 24.96012581281997 16.92561651627871 18.261820047514416 2 15.083727256510882 24.85339368533714 39.6243979664645 20.43848109168748 3 18.067137887977168 29.041428826257583 34.2702015697467 18.621231716018553 4 11.405748305387085 21.998193899393506 41.96229486264716 24.633762932572246 5 10.112046379396846 40.95824739394615 37.19382351301633 11.735882713640672 6 24.620941848019978 43.13737513378523 18.358120763467713 13.883562254727078 7 22.463097574027827 38.223555119514806 24.009431858722795 15.30391544773457 8 23.385658223332147 35.71173742418837 21.596280770602924 19.306323581876562 9 24.122034427399214 18.90050838387442 25.16667409917945 31.810783089546913 10-11 21.42068765608277 29.61949250802711 29.830204245451302 19.129615590438814 12-13 24.000657819551176 29.15785160594379 29.20356449000867 17.637926084496364 14-15 21.788597038886905 30.26946575098109 29.35861130931145 18.58332590082055 16-17 17.297594095611846 33.13943765608277 30.97629771673207 18.586670531573315 18-19 16.514393061006068 31.727724759186586 35.11890162326079 16.638980556546556 20-21 17.801239743132356 29.999386817695328 34.970901712450946 17.22847172672137 22-23 18.240653769291015 29.7178501648638 34.49597386706654 17.54552219877864 24-25 18.48086024382494 30.35798897380582 34.82532568523505 16.335825097134194 26-27 18.017041226165556 29.58077022774593 36.324234831106786 16.07795371498173 28-29 16.764360751784555 30.38968930214927 36.40770069096615 16.438249255100022 30-31 17.95770133395767 29.55220718988132 35.46276011617082 17.02733135999019 32-33 17.649944395358755 30.877717171660862 34.95845678306943 16.51388164991095 34-35 18.224614252586516 30.035620317516948 35.260490991794505 16.479274438102035 36-37 18.827762665001785 29.209496521584015 35.240423207277914 16.722317606136283 38-39 17.589970567249374 30.975461559043882 35.115835711737425 16.31873216196932 40-41 17.34469764537995 30.72405681412772 35.66686362825544 16.26438191223689 42-43 17.25700842097634 31.000119861629504 34.31220330762348 17.430668409770675 44-45 17.94282911166607 30.361721815911523 33.92877051373529 17.766678558687122 46-47 18.991370852657866 31.18254994648591 32.80999152693543 17.0160876739208 48-49 18.50890786657153 31.168335265786656 33.01429272208348 17.30846414555833 50-51 17.519733321441315 31.389359614698538 33.10905726007849 17.981849803781664 52-53 18.31464056368177 32.37323849447021 31.957946842668573 17.35417409917945 54-55 18.26307750624331 31.25 31.772319835890116 18.71460265786657 56-57 18.668613985016055 31.72187165536925 31.507815287192294 18.101699072422406 58-59 19.069969675347842 30.575499464859078 31.943174723510527 18.411356136282556 60-61 18.453164020692114 31.642157955761686 32.33839859079558 17.566279432750626 62-63 18.1783468605066 30.86592490189083 32.82922315376383 18.126505083838744 64-65 18.083303603282197 31.592824652158402 32.22328754905458 18.100584195504815 66-67 18.143228237602568 30.981593382090615 31.53763824473778 19.337540135569036 68-69 17.8639515697467 30.303190777738138 32.23833838744203 19.594519265073135 70-71 18.59168747770246 30.84223376739208 32.33031573314306 18.235763021762395 72-73 18.458180966821264 29.31847574027827 33.524627631109524 18.69871566179094 74-75 17.484614698537282 31.432003656796294 31.761171066714233 19.322210577952195 76-77 18.016968426685693 30.148780324652158 32.42647386728505 19.407777381377095 78-79 17.9018573849447 30.293993043168033 31.978014627185157 19.826134944702105 80-81 18.177789422047805 30.304026935426332 31.318286211202285 20.19989743132358 82-83 17.554015786657153 30.533970299678913 31.885479843025326 20.0265340706386 84-85 17.226520692115592 30.193375401355688 31.884364966107743 20.695738940420977 86-87 17.04256600071352 30.803213075276492 31.46266277202997 20.691558151980022 88-89 17.72375579735997 29.830761683910094 30.589992864787728 21.855489653942207 90-91 17.43712821885122 28.682131647460878 31.386830793932198 22.493909339755707 92-93 18.077272549904958 29.073643605309073 31.5355842823776 21.31349956240837 94-95 17.982407242240456 28.632268997502674 31.055732697110237 22.32959106314663 96-97 18.21290804495184 28.753233143061006 30.66496833749554 22.368890474491614 98-99 18.08776311095255 29.800938726364613 29.469262843382086 22.64203531930075 100-101 17.46261833346342 29.734174815739884 29.874669669837097 22.9285371809596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 436.0 1 278.5 2 143.0 3 159.0 4 134.5 5 139.0 6 243.5 7 385.5 8 518.0 9 617.5 10 578.5 11 471.0 12 443.0 13 412.0 14 395.5 15 412.0 16 419.5 17 470.5 18 510.0 19 544.0 20 600.5 21 669.5 22 789.5 23 909.5 24 1013.5 25 1268.5 26 1821.0 27 2675.0 28 3027.0 29 3247.0 30 4052.0 31 4703.5 32 5660.5 33 7101.0 34 8662.0 35 9496.0 36 10479.0 37 11427.5 38 10719.0 39 9632.5 40 8723.0 41 7599.5 42 6573.5 43 6109.0 44 5639.0 45 5115.0 46 4741.5 47 5159.0 48 7169.5 49 6337.5 50 3349.0 51 1974.0 52 1153.5 53 804.0 54 618.5 55 520.0 56 430.0 57 334.5 58 293.5 59 274.5 60 238.5 61 195.0 62 161.5 63 126.5 64 80.0 65 55.0 66 41.0 67 31.5 68 27.5 69 19.0 70 10.5 71 8.5 72 6.0 73 5.5 74 7.0 75 6.0 76 2.5 77 1.5 78 2.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043480199785943634 2 0.0 3 0.0 4 0.0 5 0.0011148769175882982 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006410542276132715 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.361576881912237E-4 24-25 5.574384587941491E-4 26-27 0.001951034605779522 28-29 0.00306591152336782 30-31 5.574384587941491E-4 32-33 2.7871922939707455E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01031261148769176 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010591330717088834 92-93 5.574384587941491E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.014493399928647876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179392.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.81961291473421 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98201667476725 21.762954869782376 2 9.870265824204584 5.886550124866215 3 4.144390025049538 3.707523189439886 4 2.4619583504692115 2.9365858009275776 5 1.6151344075971137 2.4081341419907245 6 1.2393913336075073 2.2174901890831253 7 0.8804725763637044 1.8378745986443095 8 0.7757879388342618 1.850695683196575 9 0.6318465622312782 1.6957277916518017 >10 4.647250158896324 27.87916963967178 >50 0.48603581710098326 9.934668212629326 >100 0.252364751187049 13.124888512308242 >500 0.005608105581934422 1.2230199785943632 >1k 0.007477474109245897 3.5347172672136997 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1865 1.0396227256510882 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1808 1.0078487334998216 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1548 0.8629147342133427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1120 0.6243310738494471 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 918 0.5117285051730289 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 704 0.392436674991081 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 572 0.31885479843025333 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 491 0.2737022832679272 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 488 0.27202996789154477 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 478 0.2664555833036033 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 476 0.265340706386015 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 469 0.2614386371744559 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 457 0.2547493756689262 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 435 0.2424857295754549 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 393 0.2190733143061006 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 371 0.2068096682126293 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 351 0.19566089903674633 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 338 0.1884141990724224 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 337 0.18785676061362824 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 311 0.17336336068498037 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.16611666072065645 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 296 0.16500178380306815 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 293 0.1633294684266857 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 259 0.14437656082768463 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 256 0.14270424545130217 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 244 0.13601498394577238 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 242 0.13490010702818409 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 241 0.13434266856938995 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 235 0.13099803781662503 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 225 0.12542365322868357 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 223 0.12430877631109526 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 214 0.11929183018194792 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 207 0.11538976097038886 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 205 0.11427488405280058 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 199 0.11093025330003567 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 197 0.10981537638244739 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 197 0.10981537638244739 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 197 0.10981537638244739 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 193 0.10758562254727078 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 193 0.10758562254727078 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 192 0.10702818408847663 No Hit GCTAGAAACCCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATC 188 0.10479843025330005 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 188 0.10479843025330005 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 187 0.1042409917945059 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 187 0.1042409917945059 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 182 0.10145379950053514 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.574384587941491E-4 0.0 24-25 0.0 0.0 0.0 0.002508473064573671 0.0 26-27 0.0 0.0 0.0 0.005295665358544417 0.0 28-29 0.0 0.0 0.0 0.01839546914020692 0.0 30-31 0.0 0.0 0.0 0.04069300749197288 0.0 32-33 0.0 0.0 0.0 0.07135212272565108 0.0 34-35 0.0 0.0 0.0 0.10786434177666786 0.0 36-37 0.0 0.0 0.0 0.14660631466286123 0.0 38-39 0.0 0.0 0.0 0.17587183374955406 0.0 40-41 0.0 0.0 0.0 0.20569479129504104 0.0 42-43 0.0 0.0 0.0 0.23914109882268997 0.0 44-45 0.0 0.0 0.0 0.26729174099179454 0.0 46-47 0.0 0.0 0.0 0.3107719407777381 0.0 48-49 0.0 0.0 0.0 0.3648434712807706 0.0 50-51 0.0 0.0 0.0 0.3980110595790225 0.0 52-53 0.0 0.0 0.0 0.459886728505173 0.0 54-55 0.0 0.0 0.0 0.6424478237602569 0.0 56-57 0.0 0.0 0.0 1.0106359257937925 0.0 58-59 0.0 0.0 0.0 1.3556903317873705 0.0 60-61 0.0 0.0 0.0 1.6360818765608278 0.0 62-63 0.0 0.0 0.0 2.020993132358188 0.0 64-65 0.0 0.0 0.0 2.4881265608276846 0.0 66-67 0.0 0.0 0.0 3.1676440420977525 0.0 68-69 0.0 0.0 0.0 3.959485372814841 0.0 70-71 0.0 0.0 0.0 4.741292811273635 0.0 72-73 0.0 0.0 0.0 5.438090884766321 0.0 74-75 0.0 0.0 0.0 6.297103549768106 0.0 76-77 0.0 0.0 0.0 7.311084106314663 0.0 78-79 0.0 0.0 0.0 8.155324652158402 0.0 80-81 0.0 0.0 0.0 8.76153897609704 0.0 82-83 0.0 0.0 0.0 9.460009364966108 0.0 84-85 0.0 0.0 0.0 10.485138690688547 0.0 86-87 0.0 0.0 0.0 11.729062611487691 0.0 88-89 0.0 0.0 0.0 13.352602122725651 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 85 1.2732926E-10 50.30563 1 TAGATTC 20 5.384517E-4 47.49763 82-83 CATATAG 115 2.4792826E-9 37.17206 3 GAGACCC 55 0.0025744787 34.54373 7 GGATATG 125 5.5988494E-9 34.198296 9 GTGGTAC 70 2.0437015E-4 33.93634 1 ACTCCCG 70 2.047054E-4 33.926876 6 GAATAAC 75 3.0640548E-4 31.673916 1 ACATATA 135 1.1857992E-8 31.665089 2 TACTCCC 75 3.069073E-4 31.665087 5 GGTACTC 80 4.4800073E-4 29.68602 3 CTCCCGC 80 4.4800073E-4 29.68602 7 TGGTACT 85 6.3878164E-4 27.939781 2 CTTTGCC 35 0.008363263 27.156645 90-91 TACACGG 70 0.008375068 27.141502 5 CGCTGTA 55 9.462031E-5 25.907797 10-11 CTTTACT 95 0.0012228774 24.998753 9 GTATAGG 135 1.3321589E-5 24.63527 1 AGGATAT 180 1.9329309E-7 23.748816 8 CTGGTTA 280 1.8189894E-12 23.748814 9 >>END_MODULE