##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937314_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174035 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00903266584308 33.0 31.0 34.0 31.0 34.0 2 32.16802367339903 33.0 31.0 34.0 31.0 34.0 3 32.14623495273939 34.0 31.0 34.0 30.0 34.0 4 35.77816531157526 37.0 35.0 37.0 35.0 37.0 5 35.78893326055104 37.0 35.0 37.0 35.0 37.0 6 35.867808199500104 37.0 35.0 37.0 35.0 37.0 7 35.85472462435717 37.0 35.0 37.0 35.0 37.0 8 35.852414744160654 37.0 35.0 37.0 35.0 37.0 9 37.50026718763467 39.0 37.0 39.0 35.0 39.0 10-11 37.41722067400235 39.0 37.0 39.0 35.0 39.0 12-13 37.350739793719654 39.0 37.0 39.0 35.0 39.0 14-15 38.54355733042204 40.0 38.0 41.0 34.5 41.0 16-17 38.51084839256471 40.0 38.0 41.0 34.5 41.0 18-19 38.566888844198004 40.0 38.0 41.0 35.0 41.0 20-21 38.56838854253455 40.0 38.0 41.0 35.0 41.0 22-23 38.45595426207372 40.0 38.0 41.0 34.5 41.0 24-25 38.30320337863074 40.0 38.0 41.0 34.0 41.0 26-27 38.12961473266871 40.0 38.0 41.0 34.0 41.0 28-29 38.16230068664349 40.0 38.0 41.0 34.0 41.0 30-31 37.98020513115178 40.0 37.5 41.0 34.0 41.0 32-33 37.71915132013675 40.0 37.0 41.0 34.0 41.0 34-35 37.56044473812739 40.0 36.5 41.0 33.0 41.0 36-37 37.48283678570402 40.0 37.0 41.0 33.0 41.0 38-39 37.350354813686906 40.0 36.0 41.0 33.0 41.0 40-41 37.19476254776338 40.0 36.0 41.0 33.0 41.0 42-43 36.8979343235556 40.0 36.0 41.0 32.5 41.0 44-45 36.55890481799638 40.0 36.0 41.0 31.0 41.0 46-47 36.03082138650271 40.0 36.0 41.0 30.0 41.0 48-49 35.57296233516247 39.5 35.0 41.0 27.5 41.0 50-51 34.66174907346223 38.5 34.5 40.0 25.5 40.5 52-53 34.74509437756773 38.5 35.0 40.0 25.0 40.5 54-55 35.06423420576321 39.0 35.0 40.5 24.0 41.0 56-57 34.88559485161031 39.0 35.0 41.0 23.0 41.0 58-59 34.67609676214555 39.0 35.0 41.0 21.5 41.0 60-61 34.378955382537995 39.0 35.0 40.0 18.5 41.0 62-63 34.0593300198236 38.5 35.0 40.0 14.0 41.0 64-65 33.71375010773695 38.0 34.0 40.0 8.0 41.0 66-67 33.316956359353 37.5 34.0 40.0 2.0 41.0 68-69 32.826494670612234 37.0 33.5 39.5 2.0 41.0 70-71 32.38716350159451 36.5 33.0 39.0 2.0 40.5 72-73 31.979521360645847 36.0 33.0 39.0 2.0 40.0 74-75 31.481417530956417 35.5 32.0 38.0 2.0 40.0 76-77 31.04792139512167 35.0 32.0 37.0 2.0 39.0 78-79 30.564274427557674 35.0 31.0 37.0 2.0 39.0 80-81 30.041560605625307 35.0 31.0 36.0 2.0 39.0 82-83 29.56669060821099 35.0 30.5 36.0 2.0 37.5 84-85 29.140448760306835 34.0 30.0 35.5 2.0 37.0 86-87 28.768029419369668 34.0 29.0 35.0 2.0 36.5 88-89 28.329080357399373 34.0 29.0 35.0 2.0 36.0 90-91 27.92541155514695 34.0 29.0 35.0 2.0 36.0 92-93 27.54876605280547 34.0 28.0 35.0 2.0 35.0 94-95 27.08700548740196 34.0 26.5 35.0 2.0 35.0 96-97 26.544054356882235 33.5 25.5 35.0 2.0 35.0 98-99 25.93203953227799 33.0 24.5 35.0 2.0 35.0 100-101 24.13444709397535 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.021592394614938115 1101 2 -0.08673358095462902 1101 3 -0.2255601072515141 1101 4 -0.05001283758310393 1101 5 -0.34631469823521144 1101 6 -0.13627647031699297 1101 7 -0.29043717822933246 1101 8 0.18450683267067802 1101 9 0.026481108701446487 1101 10-11 -0.0016610802027017257 1101 12-13 -0.066939301701467 1101 14-15 -0.1503136355477679 1101 16-17 -0.049651046400370547 1101 18-19 -0.05245821817857177 1101 20-21 0.18995719790861187 1101 22-23 0.0658355452068875 1101 24-25 0.1034207491839183 1101 26-27 -0.018203453895971222 1101 28-29 -0.06336619600735816 1101 30-31 0.02612038323085386 1101 32-33 -0.33586086491854417 1101 34-35 0.13362354037199964 1101 36-37 0.27114597303044263 1101 38-39 0.13905240880289682 1101 40-41 0.3061418613909339 1101 42-43 0.3770669135365452 1101 44-45 0.33398352509128415 1101 46-47 -0.2627459221080244 1101 48-49 -0.3195580293572817 1101 50-51 -0.12846936059512615 1101 52-53 -0.28829350345730376 1101 54-55 0.3424393138269366 1101 56-57 0.37227635484335053 1101 58-59 0.6714815004608852 1101 60-61 0.6108990472023308 1101 62-63 0.75398477120536 1101 64-65 0.49478621147449786 1101 66-67 0.6177312880241175 1101 68-69 0.7366703408317576 1101 70-71 0.6799656073276097 1101 72-73 0.7507232605026068 1101 74-75 0.8031924444772294 1101 76-77 0.6442795395061047 1101 78-79 0.7409367216079588 1101 80-81 0.5987116848806799 1101 82-83 0.5574529134040063 1101 84-85 0.8518912973770014 1101 86-87 0.38981025280814663 1101 88-89 0.7583301413929604 1101 90-91 0.6544838656181682 1101 92-93 0.44574800600908304 1101 94-95 0.5975746147446337 1101 96-97 0.8264125790845718 1101 98-99 1.0962168649927158 1101 100-101 1.2089874473324826 1102 1 0.25148661545890505 1102 2 0.30852911751424017 1102 3 0.26466095109184096 1102 4 0.09064349572663843 1102 5 0.27366312515403735 1102 6 0.23315283583819735 1102 7 0.2339217053906424 1102 8 0.41764435124949273 1102 9 0.30335845317026866 1102 10-11 0.6579279447341904 1102 12-13 0.5654479868539894 1102 14-15 0.5481552346246943 1102 16-17 0.7894505649427259 1102 18-19 0.977492002407935 1102 20-21 0.6163497239189439 1102 22-23 0.6060365502320124 1102 24-25 0.7211573193587384 1102 26-27 0.7023004741951908 1102 28-29 0.8179417521843035 1102 30-31 0.3541100970582036 1102 32-33 0.637833899985786 1102 34-35 0.7262462784756636 1102 36-37 0.6996264033510684 1102 38-39 0.8756697678988488 1102 40-41 0.7879995064236098 1102 42-43 1.1220948702213818 1102 44-45 0.9638228626240206 1102 46-47 0.7505010639962038 1102 48-49 0.6774221768380144 1102 50-51 0.6125125442341854 1102 52-53 0.8358247403438597 1102 54-55 0.7546765563777171 1102 56-57 0.8498166384208616 1102 58-59 0.7876373973160611 1102 60-61 0.8353546585926139 1102 62-63 0.8754371032883128 1102 64-65 0.8304433705091085 1102 66-67 0.8908574823761626 1102 68-69 0.693602890906071 1102 70-71 0.523962587533795 1102 72-73 0.8317504622389826 1102 74-75 1.0791732876602858 1102 76-77 1.0158439342497765 1102 78-79 1.2036729379006879 1102 80-81 1.0245615916917323 1102 82-83 1.2074871220056984 1102 84-85 1.153976842473373 1102 86-87 0.9884018021039225 1102 88-89 1.2802944228943645 1102 90-91 1.2624414941363469 1102 92-93 1.5067336855806523 1102 94-95 1.3635523909496037 1102 96-97 1.3377486019217635 1102 98-99 1.4090840176574453 1102 100-101 1.498061927715618 1103 1 -0.08401903780742614 1103 2 -0.20139027109547314 1103 3 -0.2160237222749899 1103 4 -0.4042528610572802 1103 5 -0.2965054259347397 1103 6 -0.06974184590384169 1103 7 -0.12626464301806806 1103 8 -0.255120514747162 1103 9 -0.08993614481968137 1103 10-11 -0.051727101329468894 1103 12-13 0.09330864430791763 1103 14-15 -0.15035031395152743 1103 16-17 -0.1852144266820659 1103 18-19 -0.2598012346104994 1103 20-21 -0.08333358847637129 1103 22-23 -0.2284352881264411 1103 24-25 -0.3372335935390893 1103 26-27 0.05768416347492433 1103 28-29 -0.026541078636469706 1103 30-31 -0.16578102521985016 1103 32-33 -0.09290311843967203 1103 34-35 0.04585212018420748 1103 36-37 0.49198664204453024 1103 38-39 0.4300955426057129 1103 40-41 0.27793616890266293 1103 42-43 0.4090504816116649 1103 44-45 0.391311870161708 1103 46-47 0.25133858493422423 1103 48-49 0.3788721349619735 1103 50-51 0.6797759445691938 1103 52-53 0.4072757453689846 1103 54-55 0.4169331518551047 1103 56-57 0.5535043478011019 1103 58-59 0.3742030380195729 1103 60-61 0.3504823805356736 1103 62-63 -0.07021563959275312 1103 64-65 0.6403261175777857 1103 66-67 0.5736071683058128 1103 68-69 0.99914099810875 1103 70-71 1.0012317458252582 1103 72-73 0.9665389882021422 1103 74-75 0.9640272449467169 1103 76-77 1.0696023094591567 1103 78-79 1.1296177662088986 1103 80-81 1.256868961875984 1103 82-83 1.1513863054227862 1103 84-85 0.9712795016023534 1103 86-87 0.6472559687705903 1103 88-89 0.8627902352896761 1103 90-91 1.0697200745383562 1103 92-93 0.4884783463846709 1103 94-95 0.36004527202163317 1103 96-97 0.18648446875593194 1103 98-99 0.7479920997344998 1103 100-101 0.8589287618864745 1104 1 -0.08124302257899885 1104 2 -0.30119462811747155 1104 3 -0.1679590014628083 1104 4 -0.12403395242783688 1104 5 -0.07582543439497869 1104 6 -0.07526743812042724 1104 7 0.02702427407161423 1104 8 -0.04163172456103581 1104 9 0.05750992117016551 1104 10-11 0.11767592320521914 1104 12-13 0.039123470873065 1104 14-15 0.3685133704816437 1104 16-17 0.2921544396461897 1104 18-19 0.1964038203810432 1104 20-21 0.13579976600755828 1104 22-23 0.23334823556221806 1104 24-25 -0.05833659692317639 1104 26-27 0.09640296635139833 1104 28-29 0.03854986891006007 1104 30-31 0.06285687664139772 1104 32-33 -0.2879115786765638 1104 34-35 -0.4893239584960014 1104 36-37 -0.31722179704176057 1104 38-39 -0.15848578142135494 1104 40-41 -0.24127121265401996 1104 42-43 0.05805640377747778 1104 44-45 0.09011421787744212 1104 46-47 -0.019388995438021084 1104 48-49 -0.18635100378592284 1104 50-51 -0.4216940215898646 1104 52-53 -0.7534158789964991 1104 54-55 -0.8520627237794116 1104 56-57 -1.102891062520392 1104 58-59 -1.0258207563966693 1104 60-61 -0.9204038292782073 1104 62-63 -1.441553943315263 1104 64-65 -1.1684725287843136 1104 66-67 -1.3272890880393646 1104 68-69 -1.1788941118743246 1104 70-71 -1.2858478861544356 1104 72-73 -1.3395629576523476 1104 74-75 -1.054413427642114 1104 76-77 -1.138149382588221 1104 78-79 -1.1207920265153 1104 80-81 -1.0452672479378897 1104 82-83 -1.1982726412777218 1104 84-85 -1.0408424315617744 1104 86-87 -0.582664187743795 1104 88-89 -0.5513383603110036 1104 90-91 -0.4192155219083453 1104 92-93 -0.28297628559502286 1104 94-95 -0.6961624267939364 1104 96-97 -1.123517117538487 1104 98-99 -0.6542657259846223 1104 100-101 -0.18136311628611423 1105 1 -0.324926523683132 1105 2 -0.24667628804462538 1105 3 -0.30306468272696563 1105 4 -0.3624979175544567 1105 5 -0.11244822254491282 1105 6 -0.4015744730224853 1105 7 -0.02792424753783962 1105 8 -0.3521367576850096 1105 9 -0.09073063634510703 1105 10-11 -0.1512592340978287 1105 12-13 -0.18339509862992998 1105 14-15 -0.17378781395152032 1105 16-17 -0.3638867430662458 1105 18-19 -0.6220452317856342 1105 20-21 -0.29524213367410823 1105 22-23 -0.1997011779569533 1105 24-25 0.11853971154565812 1105 26-27 -0.721314777203041 1105 28-29 -0.794726106885058 1105 30-31 -0.2710070139204177 1105 32-33 -0.25250763821368594 1105 34-35 -0.5146616510022284 1105 36-37 -0.32867896530010654 1105 38-39 0.06767499175825975 1105 40-41 -0.18617929719902548 1105 42-43 0.1346860748319969 1105 44-45 0.1907910368283794 1105 46-47 0.5959713532958091 1105 48-49 0.6305493948489769 1105 50-51 0.9762095603883978 1105 52-53 1.0342178357644656 1105 54-55 1.394201866544364 1105 56-57 1.4175138816993993 1105 58-59 1.43868926683313 1105 60-61 1.6928199511571407 1105 62-63 1.6357783575823817 1105 64-65 1.4115125582557582 1105 66-67 1.3161477756504496 1105 68-69 1.3732582297471723 1105 70-71 1.2422716469552029 1105 72-73 1.3671392706880106 1105 74-75 1.1611935585060387 1105 76-77 1.5344681286681983 1105 78-79 1.0420030063218917 1105 80-81 1.1018548375821986 1105 82-83 1.3725639184171357 1105 84-85 1.6106244874780593 1105 86-87 1.3415091467366906 1105 88-89 1.4206998398094512 1105 90-91 1.7163920378151936 1105 92-93 1.8468381627688508 1105 94-95 1.8273828426431002 1105 96-97 1.5012355280779666 1105 98-99 0.7097682296780015 1105 100-101 1.7222762195135957 1106 1 0.4555885203321104 1106 2 0.15120178316809074 1106 3 0.38935418470175165 1106 4 0.2538139994078392 1106 5 0.023867636080758814 1106 6 0.002084704483749533 1106 7 0.060993108919916494 1106 8 0.20866339998152483 1106 9 -0.0038169587731715637 1106 10-11 -0.1679456090814 1106 12-13 -0.46658718902541096 1106 14-15 0.08299513178491225 1106 16-17 0.06963053455825019 1106 18-19 0.27514304833524506 1106 20-21 0.3720322060972805 1106 22-23 0.09490531264874846 1106 24-25 -0.45218029896543754 1106 26-27 0.13611197355243831 1106 28-29 -0.08928332669848515 1106 30-31 0.07743602904371727 1106 32-33 0.4226007575293167 1106 34-35 0.18084485274234652 1106 36-37 0.31556949088174235 1106 38-39 0.29522393407858516 1106 40-41 0.0955839402205001 1106 42-43 0.5258140475031325 1106 44-45 0.8874964631849664 1106 46-47 1.128276569430355 1106 48-49 0.8230339566673948 1106 50-51 0.6708127662746151 1106 52-53 1.3295141174620042 1106 54-55 1.785937512320217 1106 56-57 1.7816414602041988 1106 58-59 2.105126003135851 1106 60-61 1.9810540859620218 1106 62-63 1.97114821699639 1106 64-65 2.441682706725075 1106 66-67 2.4800388543523297 1106 68-69 2.0851390601242556 1106 70-71 2.2757593621043313 1106 72-73 2.30205255409361 1106 74-75 2.531069396109512 1106 76-77 2.678371689304118 1106 78-79 2.8291696072941726 1106 80-81 2.3213069463721148 1106 82-83 1.9190098519344154 1106 84-85 2.291349753540338 1106 86-87 2.308169243964386 1106 88-89 2.530425894204406 1106 90-91 2.106238969887194 1106 92-93 2.5460728231288563 1106 94-95 2.1727875200836486 1106 96-97 2.147421968755932 1106 98-99 2.570545394308141 1106 100-101 2.560963645607405 1107 1 0.2322525139167091 1107 2 0.314593924306827 1107 3 0.17657685243765542 1107 4 0.3685560469886937 1107 5 0.10058509130663396 1107 6 -0.013886960846370755 1107 7 0.2627439776715903 1107 8 0.1103608070919222 1107 9 0.38150782069755707 1107 10-11 -0.1272479346627975 1107 12-13 0.22163120177918216 1107 14-15 0.4152926458185888 1107 16-17 0.178291320444373 1107 18-19 -0.016356550702447237 1107 20-21 0.2120148310638612 1107 22-23 0.4681343957815969 1107 24-25 0.4122276133574658 1107 26-27 0.659067065773776 1107 28-29 0.24608823170836303 1107 30-31 0.022437365584742963 1107 32-33 0.11111987006607649 1107 34-35 0.14382266616122052 1107 36-37 0.028714371929588367 1107 38-39 -0.31460632521037724 1107 40-41 -0.3202139747754913 1107 42-43 0.047699906899019595 1107 44-45 0.3919404621157341 1107 46-47 -0.015902794376117413 1107 48-49 0.09241380162863067 1107 50-51 -0.15894531979862592 1107 52-53 -0.4774225304930795 1107 54-55 -0.538975612512715 1107 56-57 -0.4291644740379823 1107 58-59 -0.4746654964631887 1107 60-61 -0.3316296884298495 1107 62-63 -0.2658873349950568 1107 64-65 -0.08668537667508502 1107 66-67 0.32369696715638696 1107 68-69 0.45927030845358274 1107 70-71 0.5287999929516971 1107 72-73 0.4733637008458196 1107 74-75 0.31792451506165875 1107 76-77 0.6008523094591531 1107 78-79 0.9881845765537243 1107 80-81 1.109598846933455 1107 82-83 1.1518352996756605 1107 84-85 1.251721311947179 1107 86-87 1.080355825092429 1107 88-89 0.8886523042551921 1107 90-91 0.7591058504004273 1107 92-93 1.0489129728214515 1107 94-95 1.6822435478837008 1107 96-97 1.614375991744435 1107 98-99 1.3588038813436931 1107 100-101 1.731773589472681 1108 1 0.4513914651511506 1108 2 0.2568506066165597 1108 3 0.26825882210371077 1108 4 0.21862065965277822 1108 5 0.2988779567082531 1108 6 0.4971652053782094 1108 7 0.3381707613211873 1108 8 0.5291188836749328 1108 9 0.5490246539968879 1108 10-11 0.38606567480466225 1108 12-13 0.38326488198050157 1108 14-15 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83.0 5 83.0 6 98.0 7 113.0 8 124.0 9 157.0 10 198.0 11 316.0 12 547.0 13 807.0 14 973.0 15 1413.0 16 2064.0 17 3032.0 18 2482.0 19 1302.0 20 1216.0 21 1129.0 22 1121.0 23 1236.0 24 1227.0 25 1421.0 26 1523.0 27 1725.0 28 2021.0 29 2361.0 30 2946.0 31 3792.0 32 4674.0 33 6476.0 34 9161.0 35 13945.0 36 21671.0 37 37286.0 38 38748.0 39 5744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.453822785102005 24.670121575115438 16.63313599234463 20.24291964743793 2 13.946332778261144 23.893841003509056 40.02742709635784 22.132399121871956 3 16.703636059850087 28.860324831333145 34.42301820046437 20.013020908352395 4 10.393426753941181 20.67276666359362 42.64082234719277 26.292984235272428 5 9.579482333417843 40.55074100537027 37.80740774886487 12.062368912347017 6 22.7141870105942 43.35386918470751 19.277406725011513 14.65453707968678 7 21.27588529582784 37.58167865099972 24.88557792503123 16.25685812814121 8 21.96825323714498 35.13619326719904 22.715574568044815 20.17997892761116 9 23.069306360710854 18.561140310983436 25.66563235793606 32.703920970369644 10-11 20.404391124469896 29.1163912856966 30.607162496365202 19.8720550934683 12-13 22.40678473549665 29.2977476336335 29.86199074180696 18.4334768890629 14-15 20.443816278828773 30.305184861080726 29.72256445094359 19.52843440914691 16-17 16.367723976729394 33.111393213986545 31.01778687415621 19.503095935127853 18-19 15.927152985063886 32.04744785025316 34.75834237636473 17.26705678831823 20-21 16.87203354949385 30.38566375094624 34.19279445754072 18.549508242019186 22-23 17.196913219560354 30.004576632692125 34.16499480451446 18.63351534323306 24-25 17.649056560330635 31.02299022598805 33.96095000172686 17.367003211954458 26-27 16.863058883126 30.369509029053 35.41622293730608 17.35120915051492 28-29 16.093208019689403 30.667395903798273 35.56988442550987 17.669511651002463 30-31 16.90633087767481 30.180129605799156 34.73418144236112 18.179358074164917 32-33 16.56111462951235 31.01531464102712 34.669238298117335 17.754332431343197 34-35 17.495458510506605 30.500384335243975 34.14854629152476 17.855610862724664 36-37 17.751981716969944 29.852688054434612 34.45251650711231 17.942813721483137 38-39 16.569382562543893 31.526094014437188 33.93747337639162 17.967050046627293 40-41 16.161462080874948 31.360483522809034 34.969060296445534 17.508994099870485 42-43 16.526442134713413 31.183383752759635 33.37871561428707 18.911458498239877 44-45 16.75023891492326 31.134068692358174 33.20572014139157 18.909972251326984 46-47 17.81872403876877 31.54697993338956 32.499215585806276 18.135080442035388 48-49 17.240793625204397 31.3914925957486 32.612100135878954 18.755613643168054 50-51 16.479204511758727 31.55352243644184 33.01886520861702 18.948407843182412 52-53 17.1013532968615 32.525770227469046 31.88511373452347 18.487762741145982 54-55 17.28627852882246 31.085477073305544 32.07002372124087 19.558220676631123 56-57 17.631619088185438 31.79752547767951 31.364383340864155 19.2064720932709 58-59 17.889880532667163 30.640737468305613 32.20638981865486 19.26299218037236 60-61 17.805400302766923 31.318820475332554 32.331017492560335 18.544761729340188 62-63 17.19524866235512 31.09409133689655 32.753375412662365 18.957284588085965 64-65 17.24181595798259 31.27505575940715 32.569825167278225 18.913303115332038 66-67 17.22874575490704 30.777643469751915 31.682783629770334 20.310827145570716 68-69 16.772860440085417 30.310408140947054 32.54680871882537 20.36992270014217 70-71 17.560967185795572 30.611240469604912 32.532717785177915 19.2950745594216 72-73 17.560310141774163 29.2217099241914 33.364704392523905 19.85327554151053 74-75 16.529280044209326 31.250179888210273 32.23913331299397 19.98140675458643 76-77 16.872324931423258 30.037332228046086 32.85293635099778 20.237406489532873 78-79 16.966051203822296 29.877818872594876 32.71547195878366 20.440657964799172 80-81 17.17102233479162 29.936104075523833 31.94422058484918 20.948653004835368 82-83 16.85421715180365 30.158693612538922 32.530751564199825 20.456337671457597 84-85 16.848736351968135 29.855015741641388 32.07122013548747 21.225027770903 86-87 16.715879265091864 30.162430354100472 32.11798360731225 21.003706773495416 88-89 17.42361233023763 28.992347562818193 31.52176728417623 22.06227282276794 90-91 16.963011143857067 28.01908730889667 32.69133357892798 22.32656796831829 92-93 17.25914328801501 28.37239688259063 33.18751654828646 21.1809432811079 94-95 16.99854088542641 27.338108791812843 33.18348869402051 22.47986162874024 96-97 16.845916302843147 27.493546149706305 32.6131690203674 23.047368527083147 98-99 16.60665509609047 28.582201460425466 31.773895974187827 23.037247469296236 100-101 16.484784648236722 28.149983596083782 32.600337287540505 22.76489446813899 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 407.0 1 267.5 2 143.5 3 163.5 4 154.5 5 161.0 6 230.0 7 383.0 8 586.0 9 669.0 10 555.5 11 424.0 12 403.5 13 419.5 14 402.5 15 404.0 16 421.5 17 445.5 18 479.0 19 507.0 20 587.5 21 707.5 22 813.0 23 892.5 24 986.0 25 1308.5 26 1993.0 27 2764.5 28 2993.0 29 3168.5 30 3930.5 31 4777.5 32 5611.0 33 6852.0 34 8470.5 35 9431.0 36 10269.0 37 11155.0 38 10534.5 39 9227.0 40 8075.0 41 7159.5 42 6381.5 43 5820.0 44 5379.0 45 4954.0 46 4549.5 47 4758.0 48 6801.5 49 6244.0 50 3223.0 51 1686.5 52 961.0 53 720.5 54 602.0 55 490.5 56 412.0 57 341.5 58 281.5 59 263.5 60 230.0 61 186.5 62 164.0 63 119.5 64 74.0 65 54.0 66 45.5 67 41.5 68 30.0 69 18.0 70 13.0 71 9.0 72 9.5 73 9.0 74 6.5 75 5.5 76 4.0 77 3.0 78 2.5 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 1.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32292354986066024 2 0.2781049788835579 3 0.26891142586261385 4 0.27753038181974893 5 0.2798287700749849 6 0.2039819576521964 7 0.1930646134398253 8 0.19995977820553337 9 0.1884678369293533 10-11 0.21058982388599995 12-13 0.2016835693969604 14-15 0.19363921050363433 16-17 0.19507570316315684 18-19 0.1887551354612578 20-21 0.18473295601459477 22-23 0.18760594133363978 24-25 0.17755049271698223 26-27 0.18272186629126325 28-29 0.19392650903553882 30-31 0.20369465912029192 32-33 0.19823598701410639 34-35 0.2059930473755279 36-37 0.18502025454649926 38-39 0.18214726922745425 40-41 0.17812508978079122 42-43 0.18703134426983078 44-45 0.19134082224839832 46-47 0.19450110609934781 48-49 0.2016835693969604 50-51 0.2042692561841009 52-53 0.2212198695664665 54-55 0.2016835693969604 56-57 0.1749648059298417 58-59 0.1755394029936507 60-61 0.1746775073979372 62-63 0.18128537363174074 64-65 0.17094262648317868 66-67 0.1752521044617462 68-69 0.17008073088746517 70-71 0.18071077656793172 72-73 0.1769758956531732 74-75 0.18157267216364523 76-77 0.1864567472060218 78-79 0.18243456775935876 80-81 0.18099807509983623 82-83 0.1746775073979372 84-85 0.16778234263222913 86-87 0.17180452207889219 88-89 0.17209182061079667 90-91 0.1764012985893642 92-93 0.17295371620651018 94-95 0.17209182061079667 96-97 0.1732410147384147 98-99 0.18300916482316776 100-101 0.17008073088746517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 174035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.974229321688167 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69495046933551 22.82644295687649 2 9.425666901643602 5.839055362427098 3 4.134975698438022 3.8423305656908093 4 2.3874893332838645 2.9580256844887525 5 1.6955440952769636 2.625908581607148 6 1.2002374503765816 2.2305858017065536 7 0.8644677772418655 1.874335622144971 8 0.6437131302637925 1.595081449133795 9 0.6047564278558972 1.6858677852156174 >10 4.650688235075872 28.789036688022524 >50 0.4730456720958706 10.036486913551872 >100 0.2114792416428598 11.420116643203954 >500 0.0074203242681705195 1.878357801591634 >1k 0.005565243201127889 2.3983681443387823 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1713 0.9842847703048238 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.8366133249059098 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1005 0.5774700491280489 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 976 0.5608067342775879 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.5366736575976097 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 706 0.40566552704915676 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 653 0.37521188266727956 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 412 0.2367339902893096 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 406 0.2332864079064556 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 400 0.22983882552360158 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 385 0.2212198695664665 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 366 0.21030252535409544 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 340 0.19536300169506132 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 337 0.19363921050363433 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 337 0.19363921050363433 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 334 0.19191541931220732 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 307 0.1764012985893642 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 303 0.1741029103341282 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 303 0.1741029103341282 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.16778234263222913 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 292 0.16778234263222913 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.16548395437699315 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 279 0.1603125808027121 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 277 0.15916338667509408 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 272 0.15629040135604907 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 262 0.15054443071795903 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 259 0.14882063952653202 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 249 0.14307466888844197 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 247 0.14192547476082396 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 242 0.13905248944177895 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 240 0.13790329531416093 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 236 0.13560490705892492 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 225 0.12928433935702588 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 219 0.12583675697417188 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 214 0.12296377165512684 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 204 0.11721780101703681 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 199 0.11434481569799178 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 185 0.10630045680466572 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 184 0.10572585974085671 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 178 0.1022782773580027 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 178 0.1022782773580027 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 175 0.1005544861665757 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 175 0.1005544861665757 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.8729853190450196E-4 0.0 18-19 0.0 0.0 0.0 5.745970638090039E-4 0.0 20-21 0.0 0.0 0.0 0.0011491941276180078 0.0 22-23 0.0 0.0 0.0 0.0022983882552360156 0.0 24-25 0.0 0.0 0.0 0.0037348809147585253 0.0 26-27 0.0 0.0 0.0 0.008618955957135058 0.0 28-29 0.0 0.0 0.0 0.019249001637601633 0.0 30-31 0.0 0.0 0.0 0.051139138679001356 0.0 32-33 0.0 0.0 0.0 0.0873387536989686 0.0 34-35 0.0 0.0 0.0 0.12210187605941333 0.0 36-37 0.0 0.0 0.0 0.1485333409946275 0.0 38-39 0.0 0.0 0.0 0.1706553279512742 0.0 40-41 0.0 0.0 0.0 0.19134082224839832 0.0 42-43 0.0 0.0 0.0 0.22064527250265753 0.0 44-45 0.0 0.0 0.0 0.24305455799120868 0.0 46-47 0.0 0.0 0.0 0.2801160686068894 0.0 48-49 0.0 0.0 0.0 0.3318298043496998 0.0 50-51 0.0 0.0 0.0 0.3654437325825265 0.0 52-53 0.0 0.0 0.0 0.4341080817077025 0.0 54-55 0.0 0.0 0.0 0.591834975723274 0.0 56-57 0.0 0.0 0.0 0.9012554945844227 0.0 58-59 0.0 0.0 0.0 1.1922889074036833 0.0 60-61 0.0 0.0 0.0 1.4379291521820323 0.0 62-63 0.0 0.0 0.0 1.7516591490217484 0.0 64-65 0.0 0.0 0.0 2.1679547217513715 0.0 66-67 0.0 0.0 0.0 2.8419570775993335 0.0 68-69 0.0 0.0 0.0 3.6030108886143593 0.0 70-71 0.0 0.0 0.0 4.289654379866119 0.0 72-73 0.0 0.0 0.0 5.007326112563565 0.0 74-75 0.0 0.0 0.0 5.793662194386187 0.0 76-77 0.0 0.0 0.0 6.715603183267733 0.0 78-79 0.0 0.0 0.0 7.435286005688511 0.0 80-81 0.0 0.0 0.0 7.999827620880858 0.0 82-83 0.0 0.0 0.0 8.661476139856926 0.0 84-85 0.0 0.0 0.0 9.666159105926969 0.0 86-87 0.0 0.0 0.0 10.926250466860115 0.0 88-89 0.0 0.0 0.0 12.504668601143448 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 30 1.2897328E-4 63.416405 1 GGTACTC 35 2.779171E-4 54.294273 8 TTAGGCA 80 2.234865E-7 41.59302 4 TAGGCAT 80 2.234865E-7 41.59302 5 CTTTAGG 70 4.2759075E-6 40.73244 2 CATTCCG 70 4.2843894E-6 40.720707 9 CCTTTAG 75 6.826671E-6 38.04984 1 GGATGAC 50 0.0016107892 38.005993 8 GGCATTC 75 6.8809513E-6 38.005993 7 GTGGTAC 50 0.0016107892 38.005993 6 TGGTACT 50 0.0016107892 38.005993 7 GCCATTG 55 0.0025571226 34.590763 1 ACTGCAC 55 0.0025716312 34.550903 8 TAGACAC 70 2.037373E-4 33.953487 5 GACACCA 35 2.0484926E-4 33.93392 12-13 AATAGGT 30 0.003955282 31.653423 58-59 GGTATAT 65 0.005793345 29.26911 1 TATACGT 65 0.0058097034 29.252234 5 CTGCACT 65 0.0058260984 29.23538 9 ATACGTC 65 0.0058260984 29.23538 6 >>END_MODULE