##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937314_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174035 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29649783089609 33.0 31.0 34.0 31.0 34.0 2 32.52017122992501 34.0 31.0 34.0 31.0 34.0 3 32.56066883098227 34.0 31.0 34.0 31.0 34.0 4 36.16760421754245 37.0 35.0 37.0 35.0 37.0 5 36.06513057718275 37.0 35.0 37.0 35.0 37.0 6 36.18967449076335 37.0 36.0 37.0 35.0 37.0 7 36.16022639124314 37.0 36.0 37.0 35.0 37.0 8 36.158945039790844 37.0 36.0 37.0 35.0 37.0 9 37.860493578877815 39.0 38.0 39.0 35.0 39.0 10-11 37.80793231246588 39.0 37.0 39.0 35.0 39.0 12-13 37.752475076852356 39.0 37.0 39.0 35.0 39.0 14-15 38.92258166460769 40.0 38.0 41.0 36.0 41.0 16-17 38.89490619702933 40.0 38.0 41.0 35.5 41.0 18-19 39.00792656649524 40.0 38.0 41.0 36.0 41.0 20-21 39.00002585686787 40.0 38.0 41.0 35.0 41.0 22-23 38.86465653460511 40.0 38.0 41.0 35.0 41.0 24-25 38.70220070675439 40.0 38.0 41.0 35.0 41.0 26-27 38.45959433447295 40.0 38.0 41.0 34.0 41.0 28-29 38.45786767029621 40.0 38.0 41.0 34.0 41.0 30-31 38.461682994799894 40.0 38.0 41.0 35.0 41.0 32-33 38.29651794179332 40.0 37.0 41.0 34.0 41.0 34-35 38.11911684431293 40.0 37.0 41.0 34.0 41.0 36-37 37.91578705432815 40.0 37.0 41.0 34.0 41.0 38-39 37.728514379291525 40.0 37.0 41.0 33.0 41.0 40-41 37.55938460654466 40.0 37.0 41.0 33.0 41.0 42-43 37.26338667509409 40.0 36.0 41.0 33.0 41.0 44-45 36.8294021317551 40.0 36.0 41.0 32.5 41.0 46-47 36.27003476312236 40.0 36.0 41.0 30.5 41.0 48-49 35.74305743097653 40.0 36.0 41.0 28.5 41.0 50-51 35.45942195535381 40.0 36.0 41.0 27.0 41.0 52-53 35.26610739219123 39.5 35.0 41.0 26.0 41.0 54-55 35.059304162955726 39.0 35.0 41.0 24.0 41.0 56-57 34.81155227396788 39.0 35.0 41.0 22.5 41.0 58-59 34.58317579797168 39.0 35.0 40.0 21.5 41.0 60-61 34.24054644180768 38.5 35.0 40.0 18.5 41.0 62-63 33.88342000172379 38.0 34.0 40.0 12.0 41.0 64-65 33.567607090527765 38.0 34.0 40.0 7.5 41.0 66-67 33.140247651334505 37.0 34.0 40.0 2.0 41.0 68-69 32.67554227597897 37.0 33.0 39.0 2.0 40.5 70-71 32.268848220185596 36.0 33.0 39.0 2.0 40.0 72-73 31.796983365415002 36.0 32.5 38.5 2.0 40.0 74-75 31.331674663142472 35.0 32.0 37.5 2.0 40.0 76-77 29.983853822506966 34.0 30.0 36.0 2.0 39.0 78-79 30.48531617203436 35.0 31.0 36.0 2.0 39.0 80-81 30.237558537075877 35.0 31.0 36.0 2.0 38.5 82-83 29.874441922601775 35.0 31.0 36.0 2.0 37.0 84-85 29.502370212888213 34.0 31.0 35.0 2.0 37.0 86-87 29.204407159479416 34.0 31.0 35.0 2.0 36.5 88-89 28.796586893440974 34.0 30.0 35.0 2.0 36.0 90-91 28.3836929353291 34.0 29.0 35.0 2.0 36.0 92-93 28.0331571235671 34.0 29.0 35.0 2.0 35.0 94-95 27.645729307323236 34.0 29.0 35.0 2.0 35.0 96-97 27.251799925302382 34.0 27.5 35.0 2.0 35.0 98-99 26.72828741345132 33.5 26.5 35.0 2.0 35.0 100-101 25.265357543022958 32.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04408213487737811 1101 2 -0.18717587520979606 1101 3 0.08612362762463022 1101 4 0.046590359710847906 1101 5 -0.15837081362035832 1101 6 -0.07057035726850103 1101 7 -0.17608639963950878 1101 8 -0.11894759916176412 1101 9 -0.19746548435011135 1101 10-11 -0.21164726314894722 1101 12-13 -0.1531535819072971 1101 14-15 0.15798879398867882 1101 16-17 -0.024330248124527998 1101 18-19 0.015878443462348457 1101 20-21 -0.2995522594624802 1101 22-23 -0.43713427252174597 1101 24-25 -0.21729223995814095 1101 26-27 -0.33652493143384277 1101 28-29 -0.3245473312646041 1101 30-31 -0.2621971263789291 1101 32-33 -0.31064589835790457 1101 34-35 -0.0968462556042482 1101 36-37 -0.2801859110006717 1101 38-39 0.1050574769675876 1101 40-41 -0.04808999599910635 1101 42-43 0.14887296500945268 1101 44-45 0.10414663210652719 1101 46-47 0.2789146669837166 1101 48-49 0.5295068074707459 1101 50-51 0.6005767753875375 1101 52-53 0.6548213882520528 1101 54-55 0.23315789948144783 1101 56-57 0.30990720734804 1101 58-59 0.3800396514680031 1101 60-61 0.47881917369683435 1101 62-63 0.8120252885005712 1101 64-65 1.1151561982635485 1101 66-67 0.8548684851749471 1101 68-69 0.7263984144318911 1101 70-71 0.5504294053762635 1101 72-73 0.5309696282532563 1101 74-75 0.681013440696038 1101 76-77 0.7201125474281689 1101 78-79 0.8701716419210506 1101 80-81 0.8592651954055519 1101 82-83 0.8369572621557531 1101 84-85 1.2469203750119675 1101 86-87 0.9155655943293795 1101 88-89 0.8793960059966182 1101 90-91 0.6447413228325445 1101 92-93 0.42098620246558127 1101 94-95 0.760114094746843 1101 96-97 0.817712948999219 1101 98-99 0.43321075133697207 1101 100-101 0.3607549201814777 1102 1 0.3071340398931923 1102 2 0.19008602112634065 1102 3 0.32537151023014843 1102 4 0.2266219731772381 1102 5 -0.05739645827556217 1102 6 0.10186458740793114 1102 7 0.21825620540448654 1102 8 0.07835346012527822 1102 9 0.29336896960982983 1102 10-11 0.23184956370833731 1102 12-13 0.2181395032367277 1102 14-15 0.4732151605633348 1102 16-17 0.2696867853998981 1102 18-19 0.31490880654536824 1102 20-21 0.401440969593601 1102 22-23 0.3566114514030403 1102 24-25 0.3643719495339184 1102 26-27 0.46828550572849537 1102 28-29 -0.03299567627605171 1102 30-31 0.48290160672319615 1102 32-33 0.45245363687695317 1102 34-35 0.7088017024961104 1102 36-37 0.42061858094260884 1102 38-39 0.5418659323902446 1102 40-41 0.651534888989211 1102 42-43 1.1111963188679468 1102 44-45 1.044552888741812 1102 46-47 1.3039459265680264 1102 48-49 1.2635644194744273 1102 50-51 1.0627939646200844 1102 52-53 1.1930878378710403 1102 54-55 1.2023701579569277 1102 56-57 1.248815363150527 1102 58-59 1.1402019653849962 1102 60-61 1.3445563100829858 1102 62-63 1.4634750254482185 1102 64-65 1.1930810337083742 1102 66-67 1.3224528860525737 1102 68-69 1.3287411138444511 1102 70-71 1.3740191852616022 1102 72-73 1.1830091686783852 1102 74-75 1.0273696348121604 1102 76-77 0.9594076143118819 1102 78-79 0.9441212019276541 1102 80-81 0.9975977030140193 1102 82-83 1.469957946327785 1102 84-85 1.1750823114547444 1102 86-87 1.3352107685573245 1102 88-89 1.338675973911311 1102 90-91 0.9028629167174635 1102 92-93 0.9574124652348281 1102 94-95 0.4327495726056192 1102 96-97 0.5218439325554982 1102 98-99 0.6728714963767217 1102 100-101 0.22541861423861675 1103 1 0.07226176183623778 1103 2 0.214452645916964 1103 3 0.0752668201128941 1103 4 0.2353374254385443 1103 5 0.10798775445476139 1103 6 0.24178292911647503 1103 7 0.16355561913983507 1103 8 0.17998139144856395 1103 9 0.2869828724574006 1103 10-11 0.19790379318907725 1103 12-13 0.10377863640255924 1103 14-15 0.38114020243938285 1103 16-17 0.42105560868297687 1103 18-19 0.17869387773464496 1103 20-21 0.3553773180023114 1103 22-23 0.38152769930923114 1103 24-25 0.23909713797613108 1103 26-27 0.34048504509197386 1103 28-29 0.5193493823504198 1103 30-31 0.1787506435741122 1103 32-33 0.4108916603275361 1103 34-35 0.24422622092157553 1103 36-37 0.25205440590073636 1103 38-39 0.5523872891741632 1103 40-41 0.5187747340478452 1103 42-43 0.5538392208779968 1103 44-45 0.13128865088587105 1103 46-47 0.14074712003536405 1103 48-49 0.09651825113271428 1103 50-51 -0.22769493822748643 1103 52-53 0.17785542581074054 1103 54-55 -0.017832417419960223 1103 56-57 0.10164723082222338 1103 58-59 0.5149794988858432 1103 60-61 0.49404071125551496 1103 62-63 0.7102427180780282 1103 64-65 0.6816174658691807 1103 66-67 0.3122848584143867 1103 68-69 0.3803009965915223 1103 70-71 0.23319789129175206 1103 72-73 0.6019790179246165 1103 74-75 0.6153224195692779 1103 76-77 0.7431832633906126 1103 78-79 0.8692546818271545 1103 80-81 0.9297977658280878 1103 82-83 0.862742179996129 1103 84-85 0.9815888022420118 1103 86-87 0.9963681178035557 1103 88-89 0.8681724144473257 1103 90-91 1.158018904992172 1103 92-93 1.092200991197977 1103 94-95 1.3605395642304074 1103 96-97 1.493145754163539 1103 98-99 1.5492978992242925 1103 100-101 1.4016513403692699 1104 1 0.0488374809563723 1104 2 0.13595751055316896 1104 3 0.054856498623891525 1104 4 0.07313882137763272 1104 5 -0.2404946905537031 1104 6 0.06395932505556345 1104 7 -0.001471876630048996 1104 8 0.014557322074615797 1104 9 0.04576036822728469 1104 10-11 0.12522507068484856 1104 12-13 0.11087730391525241 1104 14-15 0.17708986402990234 1104 16-17 0.059843150984164595 1104 18-19 0.21098818822533616 1104 20-21 0.1159526141106042 1104 22-23 0.2544126400875797 1104 24-25 0.28561522173247766 1104 26-27 0.11906478282463695 1104 28-29 -0.03183505081371152 1104 30-31 0.2557123610022032 1104 32-33 0.15190265863548547 1104 34-35 0.46195834867114627 1104 36-37 0.7148690209599522 1104 38-39 0.5991829744533561 1104 40-41 0.3223253897161982 1104 42-43 0.8526574315378994 1104 44-45 0.4911131009704732 1104 46-47 -0.013797613467168901 1104 48-49 0.04373430443220627 1104 50-51 0.33578327666253927 1104 52-53 -0.10847071632124283 1104 54-55 -0.04023882604939644 1104 56-57 0.34416520882560064 1104 58-59 0.2652306621684133 1104 60-61 0.21721911650086412 1104 62-63 -0.46164550104210633 1104 64-65 -0.7481096914912229 1104 66-67 -0.7006830984384038 1104 68-69 -0.7014989188061165 1104 70-71 -0.9269781873884497 1104 72-73 -0.8326656521261455 1104 74-75 -1.1446580161329223 1104 76-77 -0.9833263601288458 1104 78-79 -1.5136371328936562 1104 80-81 -1.3907526783343478 1104 82-83 -1.2977511575673226 1104 84-85 -1.1177396664551118 1104 86-87 -1.0154894329578639 1104 88-89 -0.6226535161787332 1104 90-91 -1.0090443352785599 1104 92-93 -1.5108552693773198 1104 94-95 -1.3770609010826185 1104 96-97 -1.2453932686790985 1104 98-99 -0.8285442116048074 1104 100-101 -0.4122366672449438 1105 1 -0.007717051723084012 1105 2 0.17711154422204345 1105 3 -0.11647046802269756 1105 4 0.01420395086227444 1105 5 -0.2482092794435431 1105 6 0.18016569747805988 1105 7 -0.05378195148163911 1105 8 -0.06993033171527685 1105 9 0.17716648827661174 1105 10-11 0.05357116607043366 1105 12-13 -0.030093185631336894 1105 14-15 0.3195229708009606 1105 16-17 0.0932395776095376 1105 18-19 0.09063773366685268 1105 20-21 0.2395581089627612 1105 22-23 0.15342035467647008 1105 24-25 -0.25238414733460246 1105 26-27 0.051554960346216205 1105 28-29 0.09420813941256512 1105 30-31 -0.05279949766882197 1105 32-33 0.024239117954657274 1105 34-35 -0.21159122258125507 1105 36-37 0.05983159516627268 1105 38-39 0.07692824962614253 1105 40-41 0.016523674725810622 1105 42-43 -0.05972892883951175 1105 44-45 0.35356972433219624 1105 46-47 -0.2500102952188783 1105 48-49 -0.5210223562119225 1105 50-51 -0.5752408421822821 1105 52-53 -0.3943550261666573 1105 54-55 -0.0955601716572474 1105 56-57 -0.1487941533585726 1105 58-59 -0.07484218190511882 1105 60-61 0.2629760926114457 1105 62-63 0.6344570542362291 1105 64-65 0.41025447151889693 1105 66-67 -0.06814416700934345 1105 68-69 -0.1375309243124363 1105 70-71 -0.07095994769129987 1105 72-73 -0.6162413210584354 1105 74-75 -0.17762426122168407 1105 76-77 -0.24344279593142204 1105 78-79 -0.018942705178496055 1105 80-81 -0.19480498840919935 1105 82-83 -0.042051252207262024 1105 84-85 -0.12743309041335138 1105 86-87 -0.29737305451282836 1105 88-89 0.07765334665071322 1105 90-91 -0.3886795413355131 1105 92-93 -0.04674674891501951 1105 94-95 0.36292303880668086 1105 96-97 0.2557252033160822 1105 98-99 0.26570856306610224 1105 100-101 0.16440734319412798 1106 1 0.12725207191375887 1106 2 0.1903489637464162 1106 3 0.21528619995785903 1106 4 0.15285196032226622 1106 5 0.02429104902839896 1106 6 0.03320734772112388 1106 7 0.1870536811553336 1106 8 0.21111026741755268 1106 9 0.2060720197442265 1106 10-11 0.23436212652241295 1106 12-13 0.2950940433793008 1106 14-15 0.2607429156176764 1106 16-17 0.21254058930313846 1106 18-19 0.022019943626119698 1106 20-21 0.14413700792479034 1106 22-23 0.14412459853404158 1106 24-25 0.33993289379008473 1106 26-27 0.2589685722237576 1106 28-29 0.11431062266048997 1106 30-31 0.17790277535706167 1106 32-33 0.3067249936608718 1106 34-35 0.15659298448746028 1106 36-37 0.0735781462108065 1106 38-39 0.22171868452299748 1106 40-41 0.4042519627300081 1106 42-43 0.07436974413381137 1106 44-45 0.36941849584710695 1106 46-47 0.3429636898028079 1106 48-49 0.774388890667602 1106 50-51 0.6791606819275557 1106 52-53 -0.09176666721251792 1106 54-55 0.21563415234748362 1106 56-57 0.5429020757834593 1106 58-59 0.3830148671028937 1106 60-61 0.18087452908498136 1106 62-63 0.2535445591633021 1106 64-65 0.11839072168313436 1106 66-67 0.8455334049260159 1106 68-69 0.7957564229481093 1106 70-71 0.6385394610591959 1106 72-73 0.39558366908740794 1106 74-75 0.09127929941423929 1106 76-77 0.31579711997975934 1106 78-79 0.3443636934550618 1106 80-81 0.30534282396762435 1106 82-83 0.39163026139148016 1106 84-85 0.23897736813348658 1106 86-87 0.22220387361750937 1106 88-89 0.3988167942922871 1106 90-91 0.5018900290231798 1106 92-93 0.6689936268568921 1106 94-95 0.5915230913621912 1106 96-97 0.6208710448612145 1106 98-99 0.7599931511622771 1106 100-101 0.5548126775785747 1107 1 0.019460037698308952 1107 2 0.004502933273286658 1107 3 -0.12660099597906083 1107 4 -0.1667818274350168 1107 5 0.012262179742116075 1107 6 -0.11274293295249294 1107 7 0.02813895247316367 1107 8 -0.14922119475833284 1107 9 -0.08729873673799915 1107 10-11 0.041833390890232636 1107 12-13 -0.006763748654911694 1107 14-15 0.13293617945087277 1107 16-17 0.3128479937404478 1107 18-19 0.10281384899901269 1107 20-21 0.016296858232195177 1107 22-23 -0.38715046161030386 1107 24-25 -0.5188949597250172 1107 26-27 -0.2857721388160641 1107 28-29 -0.46628280155763235 1107 30-31 -0.5211415978051903 1107 32-33 -0.2766083396724568 1107 34-35 -0.12942679631980525 1107 36-37 -0.4164980366279991 1107 38-39 -0.0722535154235402 1107 40-41 -0.07075471997514882 1107 42-43 0.15198936455615808 1107 44-45 -0.2778349123325228 1107 46-47 0.029845539575596547 1107 48-49 0.40246293504075936 1107 50-51 0.27679500430125614 1107 52-53 0.12074335684522453 1107 54-55 0.1252171515455629 1107 56-57 0.1212233802475069 1107 58-59 -0.6866189206543893 1107 60-61 -0.3483623059858587 1107 62-63 -0.5385898968644938 1107 64-65 -0.18009230424576117 1107 66-67 -0.41589402089596206 1107 68-69 -0.6868685436383686 1107 70-71 -0.7421074248002029 1107 72-73 -0.451181901615616 1107 74-75 -0.10836651721233181 1107 76-77 -0.04911199523008136 1107 78-79 -0.1333494848395098 1107 80-81 0.0805700359430297 1107 82-83 0.35654605930647065 1107 84-85 0.6100011585638541 1107 86-87 0.6205599281483813 1107 88-89 0.3830790236427255 1107 90-91 0.08492264062435595 1107 92-93 0.13925558889912537 1107 94-95 0.2680467481384561 1107 96-97 0.5717197484164132 1107 98-99 0.8640428704886638 1107 100-101 0.3994063691049021 1108 1 0.09845752120507711 1108 2 0.2791407622877671 1108 3 0.2995025203101349 1108 4 0.3240023662669458 1108 5 0.22630013118060077 1108 6 0.16097671609872322 1108 7 0.32301074734495927 1108 8 0.24688844273061505 1108 9 0.11772744839428384 1108 10-11 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-0.0888727850193689 2316 82-83 -0.07171226915641071 2316 84-85 -0.5243257457805832 2316 86-87 -0.03607361948457921 2316 88-89 -0.29240315052442867 2316 90-91 -0.0878320837083919 2316 92-93 0.13263178215842686 2316 94-95 0.08891173937164965 2316 96-97 -0.5663086949890008 2316 98-99 -0.5393761826966106 2316 100-101 -0.9612988714951385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 7.0 7 15.0 8 39.0 9 79.0 10 161.0 11 323.0 12 495.0 13 741.0 14 973.0 15 1558.0 16 2229.0 17 3496.0 18 2958.0 19 1485.0 20 1058.0 21 1006.0 22 1019.0 23 1005.0 24 1107.0 25 1174.0 26 1321.0 27 1526.0 28 1909.0 29 2315.0 30 2846.0 31 3443.0 32 4520.0 33 6078.0 34 8834.0 35 13462.0 36 21363.0 37 39027.0 38 41582.0 39 4878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.95734480003219 24.78140647186309 16.97297545888832 18.288273269216397 2 15.102709225155857 24.875456086419398 39.6041026230356 20.417732065389146 3 18.20495877266067 28.920619415634786 34.505128278794494 18.369293532910046 4 11.198896773637488 21.919728790185882 42.42077742982733 24.460597006349296 5 9.977184825897808 41.05294614584471 37.271489078025596 11.698379950231887 6 24.684115264170998 42.972965208147784 18.278507196828226 14.06441233085299 7 22.45123107420921 38.04981756543224 24.04976010572586 15.449191254632687 8 23.579739707530095 35.29117706208521 21.751371850489846 19.377711379894848 9 24.288792484270406 18.812882466170596 25.406383773378916 31.49194127618008 10-11 21.48562070847818 29.683109719309336 29.813830551325882 19.017439020886602 12-13 24.00257437235308 29.082075357854997 29.251305861868833 17.66404440792309 14-15 21.877783204527827 30.187031344269833 29.516476570804723 18.41870888039762 16-17 17.291062142672452 32.942224265234 31.221306059126036 18.545407532967506 18-19 16.35475622719568 31.62237480966472 35.41902490878272 16.60384405435688 20-21 17.732927284741574 29.9169707242796 35.17941793317436 17.170684057804465 22-23 17.979297089353487 29.72760991418319 34.65597140780367 17.637121588659653 24-25 18.494193629943748 30.21697034470474 35.072945935540965 16.215890089810554 26-27 18.12247712352578 29.481849654518555 36.51846637840633 15.877206843549338 28-29 16.765068392790962 30.468334755489668 36.39341153756647 16.373185314152895 30-31 17.70688486157877 29.382764287438086 35.75220933840514 17.158141512578002 32-33 17.737524061252046 30.815927830608786 34.98376763294739 16.46278047519177 34-35 18.31959088689057 29.898583618237712 35.417013819059385 16.364811675812337 36-37 18.88499439767863 28.98928376475996 35.43281523831413 16.69290659924728 38-39 17.55394029936507 31.00008618955957 35.17711954491913 16.268853966156232 40-41 17.31088574137386 30.66969287786939 35.985003016634586 16.03441836412216 42-43 17.221127157166418 30.930160390315663 34.54425818760667 17.304454264911243 44-45 17.896400149395237 30.29764127905306 33.94949291809119 17.85646565346051 46-47 18.977217226419974 31.061568075387136 32.991926911253486 16.969287786939407 48-49 18.43910707616284 31.136840290746115 33.32232022294367 17.10173241014738 50-51 17.53354210359985 31.15235441146896 33.364553107133624 17.949550377797568 52-53 18.365898715484576 32.41541188672361 31.960042405385142 17.25864699240668 54-55 18.363834860803863 31.27072140661361 31.818599706955496 18.54684402562703 56-57 18.675553767920245 31.55801993851811 31.782112793403627 17.984313500158013 58-59 19.12948544832936 30.549889390065214 31.9271985520154 18.393426609590023 60-61 18.581032550923666 31.697647025023702 32.30901830091648 17.41230212313615 62-63 18.217312609532566 30.852127445628753 33.02927572040107 17.901284224437614 64-65 18.15037205159882 31.587037090240464 32.29034389634269 17.972246961818026 66-67 18.202085787341627 30.96934524664579 31.5571580429224 19.271410923090183 68-69 17.953285258712327 30.26690033613928 32.33458787025598 19.445226534892406 70-71 18.704858218174504 30.80098830694975 32.40411411497687 18.09003935989887 72-73 18.69106788864309 29.176315109029794 33.64495647427241 18.487660528054704 74-75 17.66828511506306 31.186830235297496 32.04355445743673 19.101330192202717 76-77 18.048093774240815 30.090499037549918 32.6486051656276 19.212802022581666 78-79 18.02999396673083 30.29850317464878 32.05533369724481 19.616169161375584 80-81 18.290861033700118 30.275806590628324 31.48159852903152 19.951733846640042 82-83 17.5749130921941 30.642399517338465 31.946447553653 19.836239836814435 84-85 17.18964576091016 30.22265636222599 31.99298991582153 20.594707961042317 86-87 17.13649553250783 30.67974832648605 31.682132904300857 20.50162323670526 88-89 17.936334645329964 29.76901198034878 30.617404545062776 21.677248829258485 90-91 17.58259755260126 28.517453303183803 31.75182376890212 22.148125375312823 92-93 18.200910749428985 29.003203424647694 31.673968942582565 21.121916883340756 94-95 17.912201568649984 28.766914701065875 31.311805096675954 22.00907863360818 96-97 18.10009480851553 28.793058867469185 30.871376447266353 22.23546987674893 98-99 17.857686683157933 30.111502802653543 29.623085475905224 22.4077250382833 100-101 17.207177439050668 30.17455141005933 29.826023244788598 22.792247906101398 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 426.0 1 273.5 2 128.5 3 152.0 4 150.5 5 148.0 6 233.0 7 386.5 8 569.0 9 664.0 10 598.5 11 485.5 12 420.0 13 391.5 14 408.0 15 429.5 16 437.0 17 461.0 18 496.0 19 560.0 20 607.0 21 662.0 22 800.0 23 959.0 24 1059.0 25 1255.5 26 1831.5 27 2596.5 28 2919.0 29 3174.5 30 3875.0 31 4551.0 32 5584.5 33 7008.5 34 8418.5 35 9273.5 36 10073.5 37 10803.5 38 10283.5 39 9219.0 40 8310.5 41 7349.5 42 6343.5 43 5828.0 44 5516.5 45 5090.5 46 4569.5 47 4914.0 48 7057.0 49 6263.0 50 3196.5 51 1843.0 52 1069.5 53 771.0 54 614.5 55 492.0 56 408.5 57 325.0 58 276.0 59 255.0 60 220.5 61 187.5 62 156.5 63 118.5 64 87.5 65 59.5 66 35.5 67 29.0 68 20.0 69 12.5 70 10.5 71 9.0 72 7.5 73 5.0 74 5.0 75 6.0 76 6.0 77 4.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04711695923233832 2 0.0 3 0.0 4 0.0 5 0.01608871778665211 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006895164765708047 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.618955957135059E-4 24-25 0.001149194127618008 26-27 0.0014364926595225099 28-29 0.002011089723331514 30-31 5.74597063809004E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012641135403798087 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.87298531904502E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007757060361421553 92-93 0.0014364926595225099 94-95 0.0 96-97 0.0 98-99 0.002011089723331514 100-101 0.010055448616657569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 174035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.8279081793892 #Duplication Level Percentage of deduplicated Percentage of total 1 72.53375970410896 21.635303243600426 2 10.103831557858642 6.027523199356452 3 4.332415095066556 3.8768063895193494 4 2.567856523665504 3.063751544229609 5 1.6162277744601339 2.4104346826787713 6 1.1693090096511336 2.0926825063923924 7 0.943923253260388 1.9708679288648834 8 0.726242992814625 1.732984744447956 9 0.583691317832444 1.5669261930071539 >10 4.702278900425728 28.131123049961214 >50 0.46040338271271986 9.68023673399029 >100 0.2446494962532026 12.59286925043813 >500 0.007705495944982759 1.5284281897319505 >1k 0.007705495944982759 3.690062343781423 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1924 1.1055247507685237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1832 1.0526618208980951 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1538 0.8837302841382481 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1128 0.6481454879765565 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 845 0.4855345189186083 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 744 0.4275002154738989 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 565 0.3246473410520872 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 506 0.290746114287356 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.2786795759473669 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 484 0.2781049788835579 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 457 0.2625908581607148 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 454 0.2608670669692878 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 437 0.25109891688453473 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 404 0.23213721377883756 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.2194960783750395 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 372 0.21375010773694944 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 362 0.20800413709885943 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 355 0.2039819576521964 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 341 0.19593759875887032 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 319 0.18329646335507227 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 319 0.18329646335507227 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 315 0.18099807509983623 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 243 0.13962708650558797 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 242 0.13905248944177895 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 239 0.13732869825035193 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 236 0.13560490705892492 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 235 0.13503030999511592 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 230 0.13215732467607091 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 227 0.1304335334846439 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 223 0.12813514522940786 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 211 0.12123998046369984 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 204 0.11721780101703681 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 201 0.11549400982560978 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 199 0.11434481569799178 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 198 0.11377021863418278 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 195 0.11204642744275578 No Hit GCTAGAAACCCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATC 193 0.11089723331513775 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 189 0.10859884505990175 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 186 0.10687505386847472 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 185 0.10630045680466572 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 185 0.10630045680466572 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 185 0.10630045680466572 No Hit CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG 181 0.10400206854942971 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 181 0.10400206854942971 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 181 0.10400206854942971 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 180 0.10342747148562072 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 180 0.10342747148562072 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 180 0.10342747148562072 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 177 0.10170368029419369 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 177 0.10170368029419369 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 175 0.1005544861665757 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.8729853190450196E-4 0.0 18-19 0.0 0.0 0.0 5.745970638090039E-4 0.0 20-21 0.0 0.0 0.0 0.0011491941276180078 0.0 22-23 0.0 0.0 0.0 0.0022983882552360156 0.0 24-25 0.0 0.0 0.0 0.0037348809147585253 0.0 26-27 0.0 0.0 0.0 0.008618955957135058 0.0 28-29 0.0 0.0 0.0 0.019536300169506134 0.0 30-31 0.0 0.0 0.0 0.05171373574281036 0.0 32-33 0.0 0.0 0.0 0.08791335076277759 0.0 34-35 0.0 0.0 0.0 0.12325107018703135 0.0 36-37 0.0 0.0 0.0 0.15025713218605452 0.0 38-39 0.0 0.0 0.0 0.17237911914270118 0.0 40-41 0.0 0.0 0.0 0.19249001637601632 0.0 42-43 0.0 0.0 0.0 0.2212198695664665 0.0 44-45 0.0 0.0 0.0 0.24391645358692216 0.0 46-47 0.0 0.0 0.0 0.28270175539403 0.0 48-49 0.0 0.0 0.0 0.33585198379636283 0.0 50-51 0.0 0.0 0.0 0.369178613497285 0.0 52-53 0.0 0.0 0.0 0.4404286494096015 0.0 54-55 0.0 0.0 0.0 0.6004539316804092 0.0 56-57 0.0 0.0 0.0 0.9133220329244118 0.0 58-59 0.0 0.0 0.0 1.2086649237222398 0.0 60-61 0.0 0.0 0.0 1.453155974372971 0.0 62-63 0.0 0.0 0.0 1.7637256873617377 0.0 64-65 0.0 0.0 0.0 2.1803085586232656 0.0 66-67 0.0 0.0 0.0 2.860631482173126 0.0 68-69 0.0 0.0 0.0 3.627431263826242 0.0 70-71 0.0 0.0 0.0 4.31780963599276 0.0 72-73 0.0 0.0 0.0 5.037779756945442 0.0 74-75 0.0 0.0 0.0 5.827276122619013 0.0 76-77 0.0 0.0 0.0 6.76386933662769 0.0 78-79 0.0 0.0 0.0 7.493607607665124 0.0 80-81 0.0 0.0 0.0 8.060447611112707 0.0 82-83 0.0 0.0 0.0 8.722096130088776 0.0 84-85 0.0 0.0 0.0 9.742005918349758 0.0 86-87 0.0 0.0 0.0 11.019909788260982 0.0 88-89 0.0 0.0 0.0 12.6164277300543 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGATC 15 6.608806E-4 94.998566 8 CAGATCA 25 0.0050144363 56.999138 9 GGGCCTC 25 0.0050144363 56.999138 9 GACTGGT 25 0.0050144363 56.999138 7 GCCTATC 35 2.7815645E-4 54.284893 7 GTATGGT 40 5.3682906E-4 47.512936 1 CTATACA 50 2.8125029E-5 47.51293 4 ACAATCA 40 5.3759065E-4 47.499283 8 CGGAGCT 20 5.383372E-4 47.499283 68-69 ACACGGA 55 4.9463306E-5 43.181164 6 CTACACG 45 9.589671E-4 42.23372 4 CGGAGGT 45 9.603252E-4 42.221584 9 TACAATC 45 9.603252E-4 42.221584 7 CCTTGGC 60 8.2485036E-5 39.594112 2 TACACGG 50 0.001609893 38.010345 5 CGAGCCG 25 0.0016143878 37.999424 78-79 TTACGAG 25 0.0016143878 37.999424 74-75 CTTTACG 25 0.0016143878 37.999424 72-73 CACGGAG 55 0.0025738291 34.544933 7 GCCGACT 55 0.0025738291 34.544933 8 >>END_MODULE