##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937313_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 200394 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2272672834516 33.0 31.0 34.0 31.0 34.0 2 32.44581674102019 34.0 31.0 34.0 31.0 34.0 3 32.49132209547192 34.0 31.0 34.0 31.0 34.0 4 36.10534247532361 37.0 35.0 37.0 35.0 37.0 5 36.03457688353943 37.0 35.0 37.0 35.0 37.0 6 36.14222481711029 37.0 35.0 37.0 35.0 37.0 7 36.10389532620737 37.0 35.0 37.0 35.0 37.0 8 36.10893539726738 37.0 35.0 37.0 35.0 37.0 9 37.78958950866792 39.0 37.0 39.0 35.0 39.0 10-11 37.74673642923441 39.0 37.0 39.0 35.0 39.0 12-13 37.71158068604848 39.0 37.0 39.0 35.0 39.0 14-15 38.85579408565127 40.0 38.0 41.0 35.0 41.0 16-17 38.83043154984681 40.0 38.0 41.0 35.5 41.0 18-19 38.92605068016009 40.0 38.0 41.0 35.5 41.0 20-21 38.90061079673045 40.0 38.0 41.0 35.0 41.0 22-23 38.782695589688316 40.0 38.0 41.0 35.0 41.0 24-25 38.63354691258221 40.0 38.0 41.0 34.5 41.0 26-27 38.50618531492959 40.0 38.0 41.0 34.0 41.0 28-29 38.42984071379382 40.0 38.0 41.0 34.0 41.0 30-31 38.39768905256645 40.0 38.0 41.0 34.0 41.0 32-33 38.21204976196892 40.0 37.0 41.0 34.0 41.0 34-35 38.050203598910144 40.0 37.0 41.0 34.0 41.0 36-37 37.843221353932755 40.0 37.0 41.0 33.5 41.0 38-39 37.657572082996495 40.0 37.0 41.0 33.0 41.0 40-41 37.48524905935308 40.0 36.0 41.0 33.0 41.0 42-43 37.18903759593601 40.0 36.0 41.0 33.0 41.0 44-45 36.762822240186836 40.0 36.0 41.0 32.0 41.0 46-47 36.19462658562632 40.0 36.0 41.0 30.5 41.0 48-49 35.68114813816781 40.0 36.0 41.0 27.5 41.0 50-51 35.406491711328684 39.5 35.0 41.0 26.5 41.0 52-53 35.219495094663515 39.0 35.0 41.0 25.5 41.0 54-55 35.00703114863718 39.0 35.0 41.0 24.0 41.0 56-57 34.75325109534218 39.0 35.0 40.0 22.5 41.0 58-59 34.532665648672115 39.0 35.0 40.0 22.0 41.0 60-61 34.19235356347994 38.5 35.0 40.0 19.0 41.0 62-63 33.87475173907402 38.0 34.0 40.0 16.0 41.0 64-65 33.541405930317275 38.0 34.0 40.0 10.5 41.0 66-67 33.08731798357236 37.0 33.5 39.5 6.0 41.0 68-69 32.647132648682096 37.0 33.0 39.0 2.0 40.5 70-71 32.22292833118756 36.0 33.0 39.0 2.0 40.0 72-73 31.758326097587755 36.0 32.0 38.5 2.0 40.0 74-75 31.28667025958861 35.0 32.0 37.5 2.0 39.5 76-77 29.89644400530954 34.0 30.0 36.0 2.0 38.5 78-79 30.440851522500672 35.0 31.0 36.0 2.0 39.0 80-81 30.24802888310029 35.0 31.0 36.0 2.0 38.5 82-83 29.88129884128267 35.0 31.0 36.0 2.0 37.0 84-85 29.495710949429622 34.0 31.0 35.0 2.0 37.0 86-87 29.15667884267992 34.0 30.0 35.0 2.0 36.5 88-89 28.712062237392338 34.0 29.5 35.0 2.0 36.0 90-91 28.296875155942793 34.0 29.0 35.0 2.0 36.0 92-93 27.96726199387207 34.0 29.0 35.0 2.0 35.0 94-95 27.55599219537511 34.0 28.5 35.0 2.0 35.0 96-97 27.111924508717827 33.5 27.0 35.0 2.0 35.0 98-99 26.720400810403504 33.5 26.5 35.0 2.0 35.0 100-101 24.940387436749603 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.010534243737687632 1101 2 0.1686546222925287 1101 3 0.21255290291313855 1101 4 0.09920021378933797 1101 5 0.23242641925729401 1101 6 -0.007309073263428445 1101 7 -0.022140285015289862 1101 8 0.09744684927439806 1101 9 0.04681988277362592 1101 10-11 0.1425838852641661 1101 12-13 -0.03212399208366179 1101 14-15 -0.25570380810032134 1101 16-17 -0.1946056830550944 1101 18-19 -0.11818095436327525 1101 20-21 -0.1651560145123412 1101 22-23 0.23795721735982056 1101 24-25 0.30509980090853617 1101 26-27 -0.0525949265791823 1101 28-29 0.020497658376321226 1101 30-31 -0.03312941332134045 1101 32-33 0.01057784456209987 1101 34-35 -0.2484126648595364 1101 36-37 -0.13339217225645683 1101 38-39 -0.2284147633042224 1101 40-41 -0.5046474558837559 1101 42-43 -0.5039564954145348 1101 44-45 -0.3482011460391945 1101 46-47 -0.999553947816743 1101 48-49 -0.3732808456886616 1101 50-51 -0.6061890560323135 1101 52-53 -0.324317566721362 1101 54-55 -0.6514103016957407 1101 56-57 -0.5925457546891195 1101 58-59 -0.33682764216986527 1101 60-61 -0.18178945446478423 1101 62-63 -0.2426371939990517 1101 64-65 -0.11995675544779516 1101 66-67 0.10915865995671226 1101 68-69 -0.3082613743008551 1101 70-71 -0.016645550391245933 1101 72-73 -0.14853801711781855 1101 74-75 -0.4684134937317097 1101 76-77 -0.3283823823698562 1101 78-79 -0.17515412229828442 1101 80-81 0.14647492829289988 1101 82-83 0.22711578027567114 1101 84-85 -0.10503669280749861 1101 86-87 0.5374162070335196 1101 88-89 0.4250985532062117 1101 90-91 0.27784300721367217 1101 92-93 0.031169542772179426 1101 94-95 0.39558881592252604 1101 96-97 0.7654397230936354 1101 98-99 0.5448888415929147 1101 100-101 -0.1054936931596373 1102 1 0.14073849301889396 1102 2 0.15523092638871816 1102 3 0.157047133897791 1102 4 -0.0950793127406655 1102 5 -0.162013668864887 1102 6 0.03479166980068271 1102 7 0.0395078983983268 1102 8 0.24452873568640143 1102 9 0.22000236289012776 1102 10-11 0.0726929177872293 1102 12-13 0.13934085239563387 1102 14-15 0.2952931673673618 1102 16-17 0.26927729368214415 1102 18-19 -0.055225874269176245 1102 20-21 0.13824938483794114 1102 22-23 0.33885897606935345 1102 24-25 0.5131764223939115 1102 26-27 0.4140512031396426 1102 28-29 0.3918990250168477 1102 30-31 0.5521642650327223 1102 32-33 0.5844828518433829 1102 34-35 0.7150222330158229 1102 36-37 0.8189714437399616 1102 38-39 0.7102824474048575 1102 40-41 0.6258835175675728 1102 42-43 0.8733259062881658 1102 44-45 1.0035314255600696 1102 46-47 0.9125665853971014 1102 48-49 0.8795383171506543 1102 50-51 0.8472574172136405 1102 52-53 1.2505209381888278 1102 54-55 1.171906602079325 1102 56-57 1.331458203340837 1102 58-59 1.2971199326032945 1102 60-61 1.4632480721398977 1102 62-63 1.23522957721935 1102 64-65 1.3665355338617857 1102 66-67 1.3276437800045002 1102 68-69 1.4664296379839783 1102 70-71 1.1608776936997174 1102 72-73 1.2406184744246893 1102 74-75 1.3550060947825315 1102 76-77 1.3215560067866363 1102 78-79 1.3350956816672124 1102 80-81 1.659105151212131 1102 82-83 1.6926323778635108 1102 84-85 1.5867130551481416 1102 86-87 1.8232065061261622 1102 88-89 1.97670230213269 1102 90-91 1.823369686575905 1102 92-93 1.710419757476636 1102 94-95 1.9497970232336819 1102 96-97 2.2128651366330843 1102 98-99 2.3556875421642154 1102 100-101 2.7903186364770463 1103 1 0.2504234175032991 1103 2 0.2422217383292491 1103 3 0.02005435027067648 1103 4 0.053546421712589165 1103 5 0.006762702443133151 1103 6 0.05397618023090445 1103 7 0.11068578396163531 1103 8 0.05696219126430435 1103 9 0.13452974961673192 1103 10-11 -2.49627265809238E-4 1103 12-13 -0.020503778701808528 1103 14-15 0.08986314252410921 1103 16-17 0.08394861352914518 1103 18-19 -0.08633908851263783 1103 20-21 -0.09373925824734641 1103 22-23 0.1447267153335261 1103 24-25 0.08344733213010613 1103 26-27 0.3997663338624662 1103 28-29 -0.07583432408997481 1103 30-31 -0.22666179277712928 1103 32-33 0.02048190543381878 1103 34-35 0.48459122232927854 1103 36-37 0.41290259244455996 1103 38-39 0.3760224213786003 1103 40-41 0.5113975362591603 1103 42-43 0.2326657856209451 1103 44-45 0.5042510911620326 1103 46-47 0.6144771496938048 1103 48-49 0.777306367941307 1103 50-51 0.6773276092559115 1103 52-53 0.9652573236929953 1103 54-55 1.3020674917043138 1103 56-57 0.9988445356094573 1103 58-59 1.1226256702112423 1103 60-61 1.0773495745650692 1103 62-63 1.0759468767974099 1103 64-65 1.2629726962104613 1103 66-67 1.0027985574010856 1103 68-69 1.1032844035504468 1103 70-71 1.5133561037316525 1103 72-73 1.4084878699055885 1103 74-75 1.227930894656346 1103 76-77 1.068421025123321 1103 78-79 1.124697006640595 1103 80-81 1.287757700602878 1103 82-83 1.2112155238866222 1103 84-85 1.1463072820498326 1103 86-87 1.0925191761841297 1103 88-89 1.304554346771674 1103 90-91 1.2408940369051784 1103 92-93 1.5886089605209186 1103 94-95 1.453503794005396 1103 96-97 2.08596738829916 1103 98-99 2.062980810826229 1103 100-101 1.5875010963532254 1104 1 0.17194153922657307 1104 2 0.17256137817424388 1104 3 0.19137871771996373 1104 4 0.13774974972717757 1104 5 0.07017733658947378 1104 6 0.07538032885182844 1104 7 -0.1404653443720818 1104 8 -0.0679751235294006 1104 9 0.10583132244554605 1104 10-11 0.05848071237402763 1104 12-13 -0.15601779739339605 1104 14-15 -0.2323388178132504 1104 16-17 -0.29914917539267094 1104 18-19 -0.26245032626052733 1104 20-21 -0.5115190622136154 1104 22-23 -0.1268711875613988 1104 24-25 0.031885900627941055 1104 26-27 6.211321300710893E-4 1104 28-29 -0.3304844266657696 1104 30-31 0.051124849575273856 1104 32-33 -0.33901205399175893 1104 34-35 -0.37946166119021285 1104 36-37 -0.5189414923514235 1104 38-39 -0.7540892722617016 1104 40-41 -0.539970140969011 1104 42-43 -0.45217579176678413 1104 44-45 -0.26774571759110444 1104 46-47 -0.7942873818767495 1104 48-49 -0.6756227407890307 1104 50-51 -1.255325684566749 1104 52-53 -1.0251917303302633 1104 54-55 -0.9607886742405256 1104 56-57 -1.097542517046115 1104 58-59 -1.2468751276004753 1104 60-61 -1.201679374602925 1104 62-63 -1.3935653183245407 1104 64-65 -1.22894661083307 1104 66-67 -1.6668504054436823 1104 68-69 -1.6198521362261715 1104 70-71 -1.2685449676155045 1104 72-73 -1.1163126771653005 1104 74-75 -1.0999697207984056 1104 76-77 -0.9772707916079284 1104 78-79 -1.1554260843099406 1104 80-81 -1.0692060559505734 1104 82-83 -0.8610776149326185 1104 84-85 -0.8812400167876433 1104 86-87 -0.7381395883474404 1104 88-89 -0.34023708245102924 1104 90-91 -0.3683149897645315 1104 92-93 -0.6895082038282929 1104 94-95 -1.2650738216773014 1104 96-97 -0.6746207129089541 1104 98-99 -0.3909626585150079 1104 100-101 0.11780654426751624 1105 1 0.16749232989931073 1105 2 0.11220222513216527 1105 3 0.007165370091975376 1105 4 0.056216648681882475 1105 5 -0.15257795689752385 1105 6 0.09757277055646796 1105 7 0.18971423214895822 1105 8 0.045613726644816666 1105 9 -0.2893174312397946 1105 10-11 0.02311485871549479 1105 12-13 -0.41151230288790686 1105 14-15 -0.06529244954737123 1105 16-17 0.09791595459928715 1105 18-19 -0.04500192100724831 1105 20-21 0.07864443282792877 1105 22-23 -0.05959699833598364 1105 24-25 0.2514919043802877 1105 26-27 -0.06018026159815548 1105 28-29 -0.3343841854435752 1105 30-31 -0.16696681485785092 1105 32-33 -0.15734494061732818 1105 34-35 0.016223141811664732 1105 36-37 0.010340201564410734 1105 38-39 -0.15360682787682123 1105 40-41 0.05720219888625877 1105 42-43 -0.4691417526351884 1105 44-45 -0.4028301453584646 1105 46-47 -0.5225773845569961 1105 48-49 -0.591261979599004 1105 50-51 -1.1658992573354325 1105 52-53 -1.0932565922975641 1105 54-55 -1.0333027455350887 1105 56-57 -1.045599338273675 1105 58-59 -0.7955216030762173 1105 60-61 -1.1515453622737937 1105 62-63 -0.6987783979278603 1105 64-65 -0.8765209876757396 1105 66-67 -0.41899460270983013 1105 68-69 -0.513794778949304 1105 70-71 -0.6116567338980019 1105 72-73 -0.9667817203172717 1105 74-75 -0.8096320097289897 1105 76-77 -0.5901493764377364 1105 78-79 -0.7175542000965933 1105 80-81 -0.7407418372424495 1105 82-83 -0.8954651785884735 1105 84-85 -1.1340408744665496 1105 86-87 -0.7808761201083634 1105 88-89 -0.7777605346086247 1105 90-91 -0.7010542017720347 1105 92-93 -0.4492106964576159 1105 94-95 -0.5818253628190853 1105 96-97 -0.4552157276503124 1105 98-99 -0.483337356987267 1105 100-101 -0.4831315420364213 1106 1 -0.27319711763945165 1106 2 -0.37274433515685956 1106 3 -0.12282108181228324 1106 4 0.1119896428110394 1106 5 -0.04118250303631754 1106 6 -0.16187326286216575 1106 7 -0.10548372058323707 1106 8 -0.08354478607534332 1106 9 -0.19199694350835017 1106 10-11 -0.18921390840779395 1106 12-13 -0.3683465645166848 1106 14-15 -0.23167059195291273 1106 16-17 -0.01603858401278302 1106 18-19 -0.3561188862338014 1106 20-21 -0.3896680765804703 1106 22-23 -0.10799380700677119 1106 24-25 -0.7911845972003277 1106 26-27 -0.31947576061967453 1106 28-29 -0.4448011657235753 1106 30-31 -0.5235828016108286 1106 32-33 0.0811015444044969 1106 34-35 -0.6884660046583022 1106 36-37 -0.4013337409314417 1106 38-39 -0.15537560704286335 1106 40-41 -0.23028086599407516 1106 42-43 -0.6734921967116563 1106 44-45 -0.383599376127691 1106 46-47 -0.20491217305616516 1106 48-49 0.26716041991928563 1106 50-51 -0.03666547741672588 1106 52-53 -0.31774101198746507 1106 54-55 -0.5751889415308682 1106 56-57 -0.8581840138720409 1106 58-59 -1.2958226718704395 1106 60-61 -1.4155450612050018 1106 62-63 -1.2665188766272522 1106 64-65 -1.6155319766867322 1106 66-67 -1.3114377759749232 1106 68-69 -1.3815126774891198 1106 70-71 -1.3730220808797888 1106 72-73 -1.078741678167642 1106 74-75 -0.8899797441863129 1106 76-77 -0.5297098159981743 1106 78-79 -0.5772937755895917 1106 80-81 -0.41701761625795797 1106 82-83 -0.9476629807862764 1106 84-85 -1.1455567558454405 1106 86-87 -0.997194048755059 1106 88-89 -1.0941462037340237 1106 90-91 -1.4987359720565436 1106 92-93 -1.760290028084892 1106 94-95 -2.5099451881991826 1106 96-97 -2.812509119190281 1106 98-99 -1.7189613220632864 1106 100-101 -1.1591062522577111 1107 1 0.02874720405741016 1107 2 0.2389657841537911 1107 3 0.10404590890799881 1107 4 0.035940150986029096 1107 5 0.25837560566893814 1107 6 0.009447992109109293 1107 7 -0.225280450587654 1107 8 -0.12304986782523031 1107 9 0.026208977835004532 1107 10-11 0.024727761941299775 1107 12-13 -0.19150166836113414 1107 14-15 0.0550726692048471 1107 16-17 0.10612226441850225 1107 18-19 0.05375738420367071 1107 20-21 0.03490107940715603 1107 22-23 0.133632352957882 1107 24-25 -0.001237624155692174 1107 26-27 0.3211561439318018 1107 28-29 0.2295470446166874 1107 30-31 0.20368189127471936 1107 32-33 0.17884789880131535 1107 34-35 -0.06789596488808769 1107 36-37 -0.10226171265040307 1107 38-39 -0.4137273525134688 1107 40-41 -0.41927777275500944 1107 42-43 -0.18931190506340556 1107 44-45 -0.21101873096640134 1107 46-47 -0.8844526061096403 1107 48-49 -1.004182929616725 1107 50-51 -0.7810534579735062 1107 52-53 -1.1752310694333232 1107 54-55 -1.1604739614159811 1107 56-57 -1.0035220607125197 1107 58-59 -0.444121390385682 1107 60-61 -0.42433869797035584 1107 62-63 -0.37727892965420295 1107 64-65 -0.44940294442866247 1107 66-67 -0.34946252188742477 1107 68-69 -0.47760193953065766 1107 70-71 -0.36807289837868495 1107 72-73 0.04041430945940405 1107 74-75 -0.44945831245851764 1107 76-77 -0.15390336438527186 1107 78-79 -0.14669280342874558 1107 80-81 -0.03301408112760029 1107 82-83 -0.14605007756046717 1107 84-85 -0.11798273905357348 1107 86-87 -0.2127265277652235 1107 88-89 -0.1569168621520518 1107 90-91 0.06284157986568317 1107 92-93 0.3632848724895652 1107 94-95 0.17379321215432952 1107 96-97 -0.11246493006080271 1107 98-99 -0.10866525489581136 1107 100-101 0.48609922719434806 1108 1 0.17902805785295328 1108 2 0.18382214749836123 1108 3 0.22877709170369798 1108 4 0.03276249089936556 1108 5 -0.26189401397477496 1108 6 -0.015652312817870495 1108 7 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-1.906252056349036 2316 76-77 -1.661527997816357 2316 78-79 -1.608862891405284 2316 80-81 -1.8056436021442188 2316 82-83 -1.2643296474529429 2316 84-85 -1.2070345474602178 2316 86-87 -1.3657079549401985 2316 88-89 -1.67822673507483 2316 90-91 -1.613991264709096 2316 92-93 -1.770439467686387 2316 94-95 -1.8728343538280754 2316 96-97 -1.6339703064048905 2316 98-99 -1.3588118824617936 2316 100-101 -1.801779560684441 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 3.0 6 11.0 7 21.0 8 46.0 9 118.0 10 188.0 11 333.0 12 593.0 13 857.0 14 1123.0 15 1856.0 16 2519.0 17 3892.0 18 3303.0 19 1694.0 20 1212.0 21 1124.0 22 1099.0 23 1199.0 24 1307.0 25 1428.0 26 1641.0 27 1851.0 28 2217.0 29 2829.0 30 3429.0 31 4263.0 32 5564.0 33 7487.0 34 10608.0 35 15766.0 36 24977.0 37 44793.0 38 46006.0 39 5036.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.817870462363395 24.992386306335092 16.917377694124227 18.272365537177294 2 15.299360260287234 24.730780362685508 39.38790582552372 20.58195355150354 3 18.339371438266614 28.681497450023453 34.64075770731659 18.338373404393344 4 11.411519306965278 21.849456570556004 41.87650328852161 24.862520833957106 5 10.049260592025473 40.90625514690836 37.49095391863767 11.553530342428491 6 24.572092976835634 43.022745191971815 18.283481541363514 14.121680289829037 7 22.446779843707894 38.17579368643772 24.175374512210944 15.202051957643443 8 23.48174097028853 35.35984111300737 21.746160064672594 19.412257852031498 9 24.195335189676335 18.88679301775502 25.371518109324633 31.54635368324401 10-11 21.425791191353035 29.582472529117638 29.90583550405701 19.085900775472318 12-13 24.037487461761494 28.846182636598183 29.35419962372809 17.762130277912238 14-15 21.69650787947743 30.13937543040211 29.66980049302873 18.494316197091727 16-17 17.145473417367786 32.8929508867531 31.27488846971466 18.686687226164455 18-19 16.411918520514586 31.665119714163097 35.31817319879837 16.604788566523947 20-21 17.73581045340679 30.004141840574068 34.97410102098865 17.28594668503049 22-23 17.977693497679525 29.731773042566996 34.641948201008034 17.64858525874545 24-25 18.399049869381994 30.35876912175295 34.951981975463276 16.29019903340178 26-27 18.071600736567376 29.44473000014971 36.43139094461273 16.052278318670187 28-29 16.851061706215535 30.35831025276343 36.3108516106495 16.479776430371533 30-31 17.812659061648553 29.56850005489186 35.52850883758995 17.090332045869637 32-33 17.659211353633342 30.669830434044933 35.119314949549384 16.551643262772338 34-35 18.388275098056827 29.59594598640678 35.41398445063225 16.60179446490414 36-37 18.887541543159976 28.953960697426073 35.44567202610857 16.71282573330539 38-39 17.51150234038943 31.013154086449696 35.20240126949909 16.272942303661786 40-41 17.23180334740561 30.571025080591234 35.87532560855116 16.321845963452 42-43 17.29032483461309 30.818237852702712 34.46011873420059 17.431318578483605 44-45 17.981326786231126 30.129145583201094 33.977065181592266 17.912462448975518 46-47 19.035749573340517 31.07652923740232 32.95632603770572 16.931395151551442 48-49 18.521512620138328 31.044841661926004 33.07484256015649 17.358803157779175 50-51 17.559657474774692 31.157369981137162 33.44386558479795 17.8391069592902 52-53 18.30718484585367 32.25894986875855 32.06607982274918 17.3677854626386 54-55 18.15373713783846 31.146391608531193 31.96827250316876 18.73159875046159 56-57 18.66797409104065 31.642913460482845 31.679341696857193 18.00977075161931 58-59 19.25831112707965 30.251903749613263 31.979999401179676 18.509785722127408 60-61 18.58139465253451 31.546852700180644 32.34203618870824 17.529716458576605 62-63 18.207131950058386 30.75890495723425 33.06161861133567 17.972344481371696 64-65 18.149495493877062 31.503687735161733 32.29388105432298 18.05293571663822 66-67 18.272752677225863 30.988203239617953 31.355978721917822 19.383065361238362 68-69 17.858568619818957 30.26163457987764 32.40715789893909 19.472638901364313 70-71 18.65250456600497 30.956266155673323 32.35700669680729 18.034222581514417 72-73 18.755302054951745 29.18824914917612 33.39047077257803 18.665978023294112 74-75 17.59384013493418 31.45528309230815 31.88793077637055 19.062945996387118 76-77 18.149495493877062 30.20749124225276 32.37971196742417 19.263301296446002 78-79 18.04819505574019 30.39112947493438 32.06682834815414 19.493847121171292 80-81 18.19116340808607 30.27261295248361 31.56955797079753 19.966665668632793 82-83 17.618042456360968 30.656356976755788 32.045869636815475 19.679730930067766 84-85 17.452174629036808 30.209515920165874 31.891731394452332 20.446578056344986 86-87 17.188139365450063 30.801071888379894 31.42858568619819 20.582203059971853 88-89 17.936165753465673 29.88437777578171 30.724971805543078 21.454484665209534 90-91 17.59257179788543 28.763072592659135 31.581836738258684 22.06251887119675 92-93 18.204024601719127 29.138316054742567 31.633668924735208 21.0239904188031 94-95 18.151242053155283 28.6864876193898 31.326037705719735 21.83623262173518 96-97 18.22384901743565 28.786041498248448 30.9702386298991 22.019870854416798 98-99 17.960368074892465 29.828487878878608 29.86316955597473 22.3479744902542 100-101 17.34982631957199 29.727052223908007 30.061436956000957 22.861684500519043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 625.0 1 391.5 2 170.0 3 185.5 4 172.5 5 177.0 6 263.5 7 453.0 8 666.0 9 742.0 10 636.0 11 529.5 12 509.5 13 469.5 14 465.5 15 494.5 16 482.0 17 495.5 18 548.0 19 592.0 20 645.5 21 764.5 22 871.5 23 993.5 24 1124.0 25 1389.5 26 2097.5 27 3039.0 28 3393.0 29 3601.0 30 4432.0 31 5242.0 32 6395.0 33 7947.5 34 9569.5 35 10695.0 36 11696.5 37 12616.0 38 12002.5 39 10594.5 40 9429.0 41 8347.0 42 7324.0 43 6878.0 44 6376.5 45 5656.5 46 5198.5 47 5685.0 48 8116.0 49 7296.5 50 3869.0 51 2264.0 52 1261.0 53 893.0 54 704.5 55 569.5 56 475.5 57 367.5 58 337.0 59 304.5 60 249.0 61 235.5 62 203.0 63 132.0 64 89.5 65 68.5 66 37.0 67 33.0 68 37.0 69 23.5 70 15.5 71 12.0 72 7.5 73 8.5 74 7.5 75 6.0 76 6.0 77 3.0 78 1.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04840464285357845 2 0.0 3 0.0 4 0.0 5 0.01546952503567971 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003493118556443806 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0019960677465393176 24-25 2.495084683174147E-4 26-27 0.002495084683174147 28-29 0.0044911524297134645 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015220016567362297 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.001746559278221903 86-87 0.0 88-89 0.0 90-91 0.011227881074283661 92-93 7.48525404952244E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.012974440352505565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 200394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.832699581823807 #Duplication Level Percentage of deduplicated Percentage of total 1 72.72711538794371 20.969190694332166 2 10.020941864691324 5.7786161262313245 3 4.146835355405944 3.5869337405311534 4 2.50610083248239 2.890306096988932 5 1.6286193945897298 2.3478746868668723 6 1.1526679243323699 1.9940716787927784 7 0.9276726838470725 1.8723115462538797 8 0.7320998978867754 1.6886733135722625 9 0.5728724969279496 1.4865714542351567 >10 4.7854756918603645 27.729872151860835 >50 0.5053739247823603 10.020759104564009 >100 0.27172502120147457 13.160074652933721 >500 0.01384586095294138 2.3304090940846534 >1k 0.008653663095588363 4.144335658752258 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2095 1.0454404822499677 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2093 1.0444424483766979 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1816 0.9062147569288501 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1240 0.6187810014271884 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1061 0.529456969769554 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 748 0.3732646686028524 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 673 0.33583839835524015 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 570 0.28443965388185277 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 557 0.27795243370559997 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 554 0.2764553828956955 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 547 0.2729622643392517 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 512 0.25549667155703265 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 509 0.2539996207471282 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 462 0.2305458247252912 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 451 0.22505663842230808 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 436 0.2175713843727856 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 411 0.20509596095691487 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 405 0.20210185933710592 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 370 0.18463626655488688 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 364 0.1816421649350779 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 357 0.17814904637863407 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 329 0.16417657215285889 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 293 0.14621196243400503 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 292 0.14571294549737018 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 274 0.13673064063794324 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 273 0.13623162370130842 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 263 0.13124145433496012 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 256 0.12774833577851633 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 255 0.1272493188418815 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 246 0.12275816641216804 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 238 0.1187660309190894 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 234 0.11676996317255008 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 230 0.11477389542601076 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 226 0.11277782767947143 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 225 0.1122788107428366 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 224 0.11177979380620179 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 220 0.10978372605966247 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 220 0.10978372605966247 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 218 0.1087856921863928 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 218 0.1087856921863928 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 215 0.10728864137648832 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 211 0.10529257362994901 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 209 0.10429453975667935 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 205 0.10229847201014002 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 205 0.10229847201014002 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 204 0.10179945507350519 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 204 0.10179945507350519 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 203 0.10130043813687037 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 201 0.10030240426360071 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 201 0.10030240426360071 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 201 0.10030240426360071 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.485254049522441E-4 0.0 24-25 0.0 0.0 0.0 0.003493118556443806 0.0 26-27 0.0 0.0 0.0 0.005489186302983123 0.0 28-29 0.0 0.0 0.0 0.01596854197231454 0.0 30-31 0.0 0.0 0.0 0.04091938880405601 0.0 32-33 0.0 0.0 0.0 0.07360499815363733 0.0 34-35 0.0 0.0 0.0 0.11477389542601076 0.0 36-37 0.0 0.0 0.0 0.15195065720530554 0.0 38-39 0.0 0.0 0.0 0.18239069034003014 0.0 40-41 0.0 0.0 0.0 0.20309989321037558 0.0 42-43 0.0 0.0 0.0 0.22655368923221253 0.0 44-45 0.0 0.0 0.0 0.2559956884936675 0.0 46-47 0.0 0.0 0.0 0.29841212810762796 0.0 48-49 0.0 0.0 0.0 0.34032955078495364 0.0 50-51 0.0 0.0 0.0 0.3767577871592962 0.0 52-53 0.0 0.0 0.0 0.4448735990099504 0.0 54-55 0.0 0.0 0.0 0.6130423066558879 0.0 56-57 0.0 0.0 0.0 0.9354072477219877 0.0 58-59 0.0 0.0 0.0 1.2041278680998433 0.0 60-61 0.0 0.0 0.0 1.442158946874657 0.0 62-63 0.0 0.0 0.0 1.7500523967783468 0.0 64-65 0.0 0.0 0.0 2.1604938271604937 0.0 66-67 0.0 0.0 0.0 2.7795243370559994 0.0 68-69 0.0 0.0 0.0 3.527051708134974 0.0 70-71 0.0 0.0 0.0 4.2281705041069095 0.0 72-73 0.0 0.0 0.0 4.9020928770322465 0.0 74-75 0.0 0.0 0.0 5.672575027196423 0.0 76-77 0.0 0.0 0.0 6.567312394582672 0.0 78-79 0.0 0.0 0.0 7.247222970747627 0.0 80-81 0.0 0.0 0.0 7.782917652225116 0.0 82-83 0.0 0.0 0.0 8.403694721398844 0.0 84-85 0.0 0.0 0.0 9.345339680828767 0.0 86-87 0.0 0.0 0.0 10.511542261744363 0.0 88-89 0.0 0.0 0.0 12.035290477758815 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGCAA 20 0.0020701666 71.26217 9 CGATAAC 20 0.0020701666 71.26217 5 GAGTTAG 25 0.004992066 57.066715 1 AGGGTCT 25 0.0050118514 57.009735 5 TGGTACT 40 5.372939E-4 47.508114 2 GACATAT 75 1.3428507E-7 44.385227 1 TGTAGAT 65 2.575598E-6 43.85364 5 CTGGTCG 55 4.943597E-5 43.189194 9 GTGGTAC 45 9.550856E-4 42.27164 1 GTAGGGG 80 2.2515087E-7 41.559227 6 GAGTACC 50 0.001603387 38.044476 1 CATTCCG 75 6.885646E-6 38.00649 9 CATGTAG 90 5.6591307E-7 36.950752 3 CCTGGTC 65 1.3229423E-4 36.5447 8 CATGGGG 170 0.0 36.320663 6 TAGATAT 80 1.0719199E-5 35.631084 7 AATAACC 80 1.0719199E-5 35.631084 2 AGGGGTG 95 8.640782E-7 35.005978 8 GTCTAGA 55 0.0025598311 34.585888 1 GGCATTC 85 1.6236121E-5 33.535137 7 >>END_MODULE