##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937312_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 219089 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00787351259078 33.0 31.0 34.0 31.0 34.0 2 32.14407843387847 33.0 31.0 34.0 31.0 34.0 3 32.13834560384136 34.0 31.0 34.0 30.0 34.0 4 35.77253992669646 37.0 35.0 37.0 35.0 37.0 5 35.782175280365514 37.0 35.0 37.0 35.0 37.0 6 35.846340984713976 37.0 35.0 37.0 35.0 37.0 7 35.76640999776347 37.0 35.0 37.0 35.0 37.0 8 35.789733852452656 37.0 35.0 37.0 35.0 37.0 9 37.44092583379357 39.0 37.0 39.0 35.0 39.0 10-11 37.369329359301474 39.0 37.0 39.0 35.0 39.0 12-13 37.279537083103214 39.0 37.0 39.0 35.0 39.0 14-15 38.46193099607922 40.0 38.0 41.0 34.0 41.0 16-17 38.39909808342728 40.0 38.0 41.0 34.0 41.0 18-19 38.46618725723336 40.0 38.0 41.0 34.0 41.0 20-21 38.410785114725066 40.0 38.0 41.0 34.0 41.0 22-23 38.29362268301923 40.0 38.0 41.0 34.0 41.0 24-25 38.22832730077731 40.0 38.0 41.0 34.0 41.0 26-27 38.20875306382338 40.0 38.0 41.0 34.0 41.0 28-29 38.14596807690025 40.0 38.0 41.0 34.0 41.0 30-31 37.92023104765643 40.0 37.5 41.0 34.0 41.0 32-33 37.65014446183971 40.0 37.0 41.0 33.5 41.0 34-35 37.61944232709082 40.0 37.0 41.0 33.0 41.0 36-37 37.44651488664424 40.0 36.5 41.0 33.0 41.0 38-39 37.306692713919915 40.0 36.0 41.0 33.0 41.0 40-41 37.12882435905043 40.0 36.0 41.0 33.0 41.0 42-43 36.807877164074874 40.0 36.0 41.0 31.5 41.0 44-45 36.474268447982325 40.0 36.0 41.0 31.0 41.0 46-47 36.00026473259726 39.5 35.5 41.0 29.5 41.0 48-49 35.560721898406584 39.0 35.0 41.0 27.5 41.0 50-51 34.54405059131221 38.0 34.0 40.0 25.0 40.5 52-53 34.66305245813345 38.5 35.0 40.0 25.0 40.5 54-55 35.06964521267612 39.0 35.0 40.5 25.5 41.0 56-57 34.86135314872038 39.0 35.0 41.0 23.5 41.0 58-59 34.643763493374834 39.0 35.0 40.0 22.5 41.0 60-61 34.325586861960204 39.0 35.0 40.0 20.0 41.0 62-63 34.038351993938534 38.0 34.5 40.0 18.0 41.0 64-65 33.66237465139738 38.0 34.0 40.0 13.0 41.0 66-67 33.28052298381023 37.5 34.0 40.0 8.0 41.0 68-69 32.80093249775206 37.0 33.0 39.0 4.0 41.0 70-71 32.32001378435248 36.0 32.5 39.0 2.0 40.5 72-73 31.846361524312037 36.0 32.0 39.0 2.0 40.0 74-75 31.336675962736606 35.0 31.5 38.0 2.0 40.0 76-77 30.863407108526673 35.0 31.0 37.0 2.0 39.0 78-79 30.35155119608926 35.0 31.0 37.0 2.0 39.0 80-81 29.833832369493678 35.0 30.0 36.0 2.0 38.5 82-83 29.343255480649418 34.0 29.5 36.0 2.0 37.0 84-85 28.824254070263684 34.0 29.0 35.0 2.0 37.0 86-87 28.427182560511937 34.0 29.0 35.0 2.0 36.0 88-89 27.945211763256026 34.0 28.5 35.0 2.0 36.0 90-91 27.4885822656546 34.0 26.5 35.0 2.0 36.0 92-93 27.065802025660805 34.0 26.0 35.0 2.0 35.0 94-95 26.581092615329844 33.5 25.0 35.0 2.0 35.0 96-97 25.916136821109227 33.0 24.0 35.0 2.0 35.0 98-99 25.153830178603215 32.5 18.0 35.0 2.0 35.0 100-101 22.701488892641805 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.21389636107393883 1101 2 -0.08693890358789957 1101 3 0.10256134569338826 1101 4 0.12855958840787451 1101 5 -0.014045312012711975 1101 6 0.07717939696644294 1101 7 -0.13640737245547285 1101 8 -0.04590193860541092 1101 9 -0.11191175079625992 1101 10-11 -0.16448849116400766 1101 12-13 -0.09828804328687113 1101 14-15 -0.03157423087235145 1101 16-17 -0.0276463173417838 1101 18-19 -0.34457357730318705 1101 20-21 -0.3369315354824778 1101 22-23 -0.09888154598538534 1101 24-25 -0.03277300489879309 1101 26-27 0.010044004690108466 1101 28-29 -0.10948696341723974 1101 30-31 -0.3106023739343442 1101 32-33 -0.2816922089174554 1101 34-35 -0.4781091615156967 1101 36-37 -0.28918328323238995 1101 38-39 -0.5944631070868169 1101 40-41 -0.40201135766075424 1101 42-43 -0.7254829023002358 1101 44-45 -0.29283835157556837 1101 46-47 -0.2726747673424512 1101 48-49 -0.5525915912300974 1101 50-51 -0.4210201924685464 1101 52-53 -0.17505685509537017 1101 54-55 -0.41339996501927345 1101 56-57 -0.46086591711427616 1101 58-59 -0.3363018383484899 1101 60-61 -0.21939392345999664 1101 62-63 -0.335499402838046 1101 64-65 -0.3840134240233297 1101 66-67 -0.3387968836290369 1101 68-69 0.024943686529447007 1101 70-71 0.20631296393694498 1101 72-73 0.24236009673677827 1101 74-75 -0.14069493336591066 1101 76-77 0.05306440073850993 1101 78-79 0.037164602773192 1101 80-81 0.3657869083405103 1101 82-83 0.4562730038318854 1101 84-85 0.0040468922894518755 1101 86-87 -0.15360825645728227 1101 88-89 -0.1673433210871238 1101 90-91 0.3323234321768105 1101 92-93 0.11900761999355325 1101 94-95 -0.015466075858018513 1101 96-97 -0.30345210384879095 1101 98-99 -0.2933454377942617 1101 100-101 0.34166575312168845 1102 1 -0.3295717963261957 1102 2 -0.23257367293597397 1102 3 -0.14156116034295962 1102 4 -0.3148258481151913 1102 5 -0.0937250680172852 1102 6 -0.2049720835266342 1102 7 -0.11759939177177614 1102 8 -0.16688300354378072 1102 9 -0.16801799047093624 1102 10-11 -0.028146516326032156 1102 12-13 -0.02988672164498496 1102 14-15 7.837561653900593E-4 1102 16-17 -0.2853664850886375 1102 18-19 -0.21895405766918685 1102 20-21 0.08642276620769707 1102 22-23 -0.18429582988422766 1102 24-25 0.15589455133453356 1102 26-27 -0.39188127171095033 1102 28-29 -0.34995589212481804 1102 30-31 -0.3314913051835475 1102 32-33 -0.6462080304957993 1102 34-35 -0.5008089963104609 1102 36-37 -0.06266148884936484 1102 38-39 -0.030191916338317526 1102 40-41 -0.4420577422077159 1102 42-43 -0.26937348560179686 1102 44-45 -0.22839242452003106 1102 46-47 -0.4507628344411927 1102 48-49 -0.33196317592955893 1102 50-51 -0.23894496054580827 1102 52-53 -0.03005749050012696 1102 54-55 -0.03587423112678323 1102 56-57 0.03365371689258723 1102 58-59 -0.05648547032205897 1102 60-61 0.23112393141355625 1102 62-63 0.3358216036430832 1102 64-65 0.2909929300241956 1102 66-67 -0.2234073805155603 1102 68-69 -0.4010475448849604 1102 70-71 0.04638158765025224 1102 72-73 -0.2805938999591149 1102 74-75 -0.23033465887106175 1102 76-77 -0.5279814754846441 1102 78-79 -0.3032376084353494 1102 80-81 -0.029117145541810885 1102 82-83 -0.5237594018105085 1102 84-85 -0.18118947827860055 1102 86-87 -0.47958677977663555 1102 88-89 -0.5112720286738579 1102 90-91 -0.3953262056424762 1102 92-93 -0.19200712139671694 1102 94-95 -0.5890141759978071 1102 96-97 -0.4897051220213662 1102 98-99 -0.8375060681157755 1102 100-101 -0.2666707572354099 1103 1 0.4466986601768177 1103 2 0.4230034157806486 1103 3 0.40333548048391066 1103 4 0.21553590899075203 1103 5 0.4263007717759919 1103 6 -0.008051325559364386 1103 7 0.3510698588742187 1103 8 0.4792194943029031 1103 9 0.20267537731545815 1103 10-11 0.3361945689452881 1103 12-13 0.4813643550130706 1103 14-15 0.27770020750044466 1103 16-17 0.3796851944979167 1103 18-19 0.05903905171927448 1103 20-21 0.4086665215909875 1103 22-23 0.43618220629148396 1103 24-25 0.5118915366919836 1103 26-27 0.40538401683340197 1103 28-29 0.7242258477545889 1103 30-31 0.5795767396054217 1103 32-33 0.3923363278159968 1103 34-35 0.5113034067903186 1103 36-37 0.4335702079637258 1103 38-39 0.027463122421373498 1103 40-41 0.5455068572754911 1103 42-43 0.47195941706831235 1103 44-45 0.6967260078658484 1103 46-47 1.1203504299860327 1103 48-49 0.9643331203667458 1103 50-51 1.0537445312715903 1103 52-53 0.9993891072604271 1103 54-55 1.0944552262375637 1103 56-57 0.8763001423878478 1103 58-59 1.148025726921695 1103 60-61 1.1302949047124358 1103 62-63 0.9705115261237083 1103 64-65 0.6909713968631266 1103 66-67 1.1725874515257857 1103 68-69 1.06110792453795 1103 70-71 1.4471675480292348 1103 72-73 1.1883983481029006 1103 74-75 1.142888855340825 1103 76-77 1.0313962161604877 1103 78-79 0.6721069221417437 1103 80-81 0.8762984444668049 1103 82-83 0.8969985654590893 1103 84-85 0.5576671108686888 1103 86-87 1.150581178879694 1103 88-89 0.67509748037007 1103 90-91 0.8622642336340078 1103 92-93 0.9707835762777073 1103 94-95 0.25282475595740195 1103 96-97 0.8637078840079191 1103 98-99 1.1570568087145396 1103 100-101 1.5796363056414222 1104 1 -0.11457348153010471 1104 2 -0.30618337971049314 1104 3 -0.0062055823180173775 1104 4 -0.07407761757929876 1104 5 -0.27249149875540724 1104 6 -0.11066807274132628 1104 7 -0.37489632470065715 1104 8 -0.09280143967455246 1104 9 -0.3533230123785813 1104 10-11 -0.4000373151126837 1104 12-13 -0.2011034388677757 1104 14-15 -0.11787145111469499 1104 16-17 -0.2668123900431425 1104 18-19 -0.05070329932742368 1104 20-21 -0.13573766520450192 1104 22-23 -0.3378516101336402 1104 24-25 -0.2422348723257315 1104 26-27 -0.21953546786868827 1104 28-29 0.11460426965475534 1104 30-31 -2.1392062002689727E-4 1104 32-33 -0.15540924384261956 1104 34-35 -0.022211085963306232 1104 36-37 -0.07806425258377914 1104 38-39 -0.13394992105688885 1104 40-41 0.04725806500496077 1104 42-43 -0.0023360740750035802 1104 44-45 -0.08469508714220808 1104 46-47 -0.47508032685777124 1104 48-49 -0.656600857088975 1104 50-51 -0.5056958874079598 1104 52-53 -0.9833604901630864 1104 54-55 -0.9151462230378016 1104 56-57 -0.7829792456958842 1104 58-59 -0.7914871555259637 1104 60-61 -0.09432264762251208 1104 62-63 -0.21245948836905626 1104 64-65 -0.5738031603027238 1104 66-67 -0.2008425001650309 1104 68-69 -0.401553106057861 1104 70-71 -0.13167705744580616 1104 72-73 -0.284065420013043 1104 74-75 -0.03579471953839786 1104 76-77 -0.22558848593291003 1104 78-79 0.08205056143320988 1104 80-81 -0.05862506600018591 1104 82-83 -0.5400384715779474 1104 84-85 -0.3990357876820383 1104 86-87 0.17727200013573352 1104 88-89 -0.16197981431592012 1104 90-91 -0.13622610453024464 1104 92-93 0.0026676342487199634 1104 94-95 0.012687879950963321 1104 96-97 0.3033012817534875 1104 98-99 0.7018434431750897 1104 100-101 0.7957997516961726 1105 1 -0.3218198583416978 1105 2 0.003532888915756871 1105 3 -0.3781590422077912 1105 4 -0.3717097874098698 1105 5 -0.18128921257455488 1105 6 -0.11375033442738669 1105 7 -0.5587501724246877 1105 8 -0.27306271748634714 1105 9 -0.08416604156821705 1105 10-11 -0.13094158128946276 1105 12-13 -0.1466025203129675 1105 14-15 -0.02915946907754119 1105 16-17 -0.33256050190270514 1105 18-19 -0.43417951896408624 1105 20-21 -0.5941373386072399 1105 22-23 -0.6528240534894252 1105 24-25 -0.49687867718386514 1105 26-27 -0.44548974425162413 1105 28-29 -0.5954794208943426 1105 30-31 -0.5407936307649521 1105 32-33 -0.48142475721269307 1105 34-35 -0.547675466449121 1105 36-37 -0.2345961978787372 1105 38-39 -0.12013077429224239 1105 40-41 -0.6468835965586237 1105 42-43 -0.35038669665103583 1105 44-45 -0.07534624043880456 1105 46-47 0.06815298606323239 1105 48-49 0.13165184754462445 1105 50-51 0.22846414525176328 1105 52-53 -0.024052468117766068 1105 54-55 0.021391845955868405 1105 56-57 0.15913138912754476 1105 58-59 0.01582045827276346 1105 60-61 0.14806355364305546 1105 62-63 -0.0019063142518831455 1105 64-65 0.36005068742129964 1105 66-67 -0.21082412906889658 1105 68-69 -0.20222671291641348 1105 70-71 -0.43901528973810855 1105 72-73 -0.151977238751563 1105 74-75 -0.15630911023037797 1105 76-77 -0.19887895337524242 1105 78-79 -0.11708191458230388 1105 80-81 -0.0462041637752435 1105 82-83 -0.21280842462958915 1105 84-85 -0.18675777176042274 1105 86-87 -0.041711465877735776 1105 88-89 0.009800140776953015 1105 90-91 0.02824623676389848 1105 92-93 -0.13250280869882047 1105 94-95 0.2634533422902159 1105 96-97 -0.46587610138592694 1105 98-99 -1.2008918089171736 1105 100-101 -0.1767864903940506 1106 1 -0.2969442364511501 1106 2 -0.008519525070646239 1106 3 -0.26975738850447684 1106 4 0.1288858260720147 1106 5 0.2020055810628918 1106 6 -0.06063562627799257 1106 7 -0.016882048256789517 1106 8 -0.2828728218938892 1106 9 -0.2475042799537519 1106 10-11 -0.5394273216019769 1106 12-13 -0.4528879943512294 1106 14-15 -0.281033899741125 1106 16-17 -0.1652982217541492 1106 18-19 -0.5915966586179238 1106 20-21 -0.26788129488220136 1106 22-23 -0.5130300656819813 1106 24-25 -0.3355725318541758 1106 26-27 -0.7447680008559274 1106 28-29 -0.5070483368778085 1106 30-31 -0.6179080586256447 1106 32-33 -0.37410922221164356 1106 34-35 -0.14945529967734927 1106 36-37 -0.4674109971219593 1106 38-39 -0.02073339499155935 1106 40-41 0.4291096765126383 1106 42-43 0.3809215508438797 1106 44-45 0.3272884733163721 1106 46-47 0.5029858639274352 1106 48-49 0.7861960280505471 1106 50-51 0.7133285555944155 1106 52-53 0.5990643421968542 1106 54-55 0.8292189078295991 1106 56-57 0.4868239124265443 1106 58-59 0.4127092537320891 1106 60-61 0.38364620377269176 1106 62-63 0.11914336691972238 1106 64-65 0.2236552000693237 1106 66-67 0.27540668867337814 1106 68-69 0.1608522584240717 1106 70-71 -0.056736542628591735 1106 72-73 -0.189711104633421 1106 74-75 0.3511185402496153 1106 76-77 0.09486360145623252 1106 78-79 -0.1860329341324416 1106 80-81 -0.1334790569151778 1106 82-83 -0.04551110839387107 1106 84-85 -0.7797085110218624 1106 86-87 -0.06480024732262635 1106 88-89 0.23956680043640333 1106 90-91 -0.2209654512747825 1106 92-93 -0.16603234412030687 1106 94-95 0.1872498526959312 1106 96-97 0.040290249955955204 1106 98-99 -0.020631140428630346 1106 100-101 0.3627692520206338 1107 1 0.18542651846989244 1107 2 0.30202918067598006 1107 3 0.2614272679235299 1107 4 0.3351011263820567 1107 5 0.48789497467454623 1107 6 0.11976670986737048 1107 7 0.1511906318210805 1107 8 0.36951740090515983 1107 9 0.3317011422107896 1107 10-11 0.27580809551533036 1107 12-13 0.214355498330292 1107 14-15 0.366669611687243 1107 16-17 0.026424324932698084 1107 18-19 0.12345129004455657 1107 20-21 0.13431064397424564 1107 22-23 0.21456384880355728 1107 24-25 -0.0683218288474734 1107 26-27 0.25220366256609594 1107 28-29 -0.15882568203607406 1107 30-31 0.07756385715775593 1107 32-33 0.10811732620568648 1107 34-35 -0.025834274369103127 1107 36-37 0.2501966169814338 1107 38-39 0.37619500647934245 1107 40-41 0.43228221959433455 1107 42-43 0.3633644090167891 1107 44-45 -0.1648883238571841 1107 46-47 -0.21227839449062458 1107 48-49 -0.22689071216143475 1107 50-51 -0.40207269900216147 1107 52-53 -0.36282908919690016 1107 54-55 -0.11901095733731637 1107 56-57 -0.3907087142949166 1107 58-59 -0.721438846347219 1107 60-61 -0.5612308601828957 1107 62-63 -0.37429523716131996 1107 64-65 -0.1759770733462105 1107 66-67 -0.4071226934017531 1107 68-69 -0.6938236374588307 1107 70-71 -0.4792615985086073 1107 72-73 -0.8454180770178752 1107 74-75 -0.8628478596350213 1107 76-77 -0.8799363120198649 1107 78-79 -0.677128182528147 1107 80-81 -1.1453400418455963 1107 82-83 -1.014434606843647 1107 84-85 -0.9722241934791391 1107 86-87 -0.7739357293328659 1107 88-89 -0.8689846452337946 1107 90-91 -0.8113468774771064 1107 92-93 -0.613757486524225 1107 94-95 -0.6757179171504895 1107 96-97 -0.5933170757344328 1107 98-99 -0.6822145278394025 1107 100-101 -0.5839103932313634 1108 1 0.23747501345317445 1108 2 0.299962105239338 1108 3 0.3269465915950249 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0.4494823628440443 2316 72-73 0.5305036112607979 2316 74-75 0.1710321301946287 2316 76-77 0.025670095302785967 2316 78-79 0.2571459084965184 2316 80-81 0.356439770002865 2316 82-83 0.4432948617553869 2316 84-85 0.04779868981605517 2316 86-87 -0.18348314860568493 2316 88-89 0.020857714288197826 2316 90-91 -0.12859833946540888 2316 92-93 0.1725379622426182 2316 94-95 0.5771424947488235 2316 96-97 0.8036445311814084 2316 98-99 0.2336893623207814 2316 100-101 -0.670485526717254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 916.0 3 163.0 4 98.0 5 97.0 6 123.0 7 143.0 8 170.0 9 159.0 10 278.0 11 391.0 12 669.0 13 968.0 14 1157.0 15 1820.0 16 2447.0 17 3643.0 18 2950.0 19 1526.0 20 1513.0 21 1438.0 22 1498.0 23 1610.0 24 1746.0 25 1961.0 26 2129.0 27 2410.0 28 2906.0 29 3486.0 30 4298.0 31 5219.0 32 6875.0 33 9002.0 34 12474.0 35 18730.0 36 28439.0 37 46211.0 38 43941.0 39 5485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.45509782031807 24.54978862026683 16.6761223257261 20.318991233688987 2 14.102036335693507 23.91596684779626 39.892420001097754 22.08957681541248 3 16.814394043339462 28.846628797233887 34.201860541697535 20.137116617729113 4 10.527687951956018 20.701815370964127 42.55030118966487 26.220195487414983 5 9.60300036589828 40.490303695572635 37.869099890230515 12.037596048298573 6 23.149105549709475 43.001326806057556 19.218556984032574 14.631010660200394 7 21.40569525273599 37.42816880787673 25.02882398155265 16.137311957834633 8 22.2304057237748 35.18026322410646 22.651728980727093 19.937602071391648 9 23.087796665995718 18.56941572581383 25.758476824827536 32.584310783362916 10-11 20.617488413800046 29.19974013972065 30.406855187369324 19.77591625910998 12-13 22.6198128378332 28.959811145092996 29.836376071973486 18.58399994510032 14-15 20.558845257454433 30.388611761046423 29.446072141675987 19.606470839823157 16-17 16.659918611593387 32.91060771382757 31.06369935241546 19.36577432216359 18-19 16.177610858113965 32.118032126952464 34.212025077595534 17.492331937338033 20-21 17.31715293993875 30.509540592401834 33.69865770737609 18.474648760283323 22-23 17.47646536818319 30.12753071623958 33.69683551831081 18.699168397266412 24-25 17.807401512136888 31.069930613682416 33.57346396439663 17.549203909784065 26-27 17.332433699667213 30.254680191667717 34.89976327436158 17.513122834303488 28-29 16.37513581517699 30.87047521015612 34.96403042259965 17.79035855206725 30-31 17.242657150699973 30.221657974197093 34.339143562997535 18.196541312105406 32-33 16.7879772171009 31.209826840817072 34.13912208065512 17.863073861426905 34-35 17.867371869366693 30.442749558485765 33.788649652736474 17.901228919411068 36-37 18.053986304440308 29.82260727959709 34.03030954526534 18.093096870697266 38-39 16.850360564552833 31.496851778579288 33.52484155972472 18.12794609714316 40-41 16.5006357657089 31.274184253135378 34.44683809471537 17.77834188644035 42-43 16.719079845665107 31.152640137593174 33.05438086503473 19.073899151706993 44-45 17.097831631777723 30.949630446369547 32.94391614758354 19.008621774269187 46-47 18.070409421475127 31.486357574079964 32.20834124480417 18.234891759640746 48-49 17.561242324691843 31.38571912261276 32.225084416951105 18.82795413574429 50-51 16.722487600431926 31.515721371181755 32.788850454803345 18.97294057358297 52-53 17.25276460211009 32.521971522252954 31.633958214637918 18.591305660999033 54-55 17.514652928069108 31.036068394057388 31.841350311360422 19.607928366513082 56-57 17.90548112504174 31.795831179702045 31.158661257049808 19.14002643820641 58-59 18.12866433125097 30.57046949172696 31.96142905488837 19.339437122133702 60-61 17.95415881610478 31.327400442680226 32.143706440814384 18.574734300400607 62-63 17.479551166103757 31.04609762846121 32.50249822208909 18.97185298334595 64-65 17.511198304305776 31.194051350999104 32.25866196555106 19.036088379144058 66-67 17.436907056668748 30.70123087546961 31.528064555222475 20.33379751263917 68-69 17.07902679959755 30.388045367236806 32.184898929845424 20.348028903320223 70-71 17.859666373209244 30.72682162743103 32.116895144232416 19.29661685512731 72-73 17.69981523150943 29.304557012970385 33.09208239577045 19.90354535974974 74-75 16.72120725652829 31.38610850032816 31.863367246832187 20.029316996311366 76-77 17.092382851810513 30.198250782207747 32.47237114156588 20.23699522441586 78-79 17.177011935881513 29.974179708315322 32.31744550637731 20.531362849425847 80-81 17.511028579400055 29.97944076126356 31.810790970400642 20.698739688935746 82-83 17.112848528365916 30.185477803719618 32.34233471986243 20.359338948052034 84-85 16.974919049448438 29.89727969558934 31.85724347364762 21.2705577813146 86-87 17.04584436790757 30.12015679386729 31.918849568450952 20.915149269774187 88-89 17.80959132801244 29.219932764653418 31.152148558099114 21.818327349235027 90-91 17.182228829550002 28.269197781462633 32.36663045342787 22.181942935559494 92-93 17.63949871935602 28.600210391511162 32.633781558726675 21.126509330406147 94-95 17.253239298765568 27.75826347058904 32.78929579268488 22.19920143796051 96-97 17.137218040813902 27.667048596579097 32.36615872275319 22.829574639853814 98-99 16.91131736280425 28.393356235505074 31.581896610875027 23.11342979081565 100-101 16.943075674254292 27.638309305223686 32.69713148959036 22.721483530931668 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 532.0 1 346.0 2 176.5 3 209.5 4 185.5 5 176.5 6 279.5 7 452.0 8 654.0 9 779.5 10 662.0 11 499.0 12 488.0 13 483.5 14 477.0 15 485.0 16 501.5 17 508.0 18 526.0 19 577.5 20 648.0 21 759.0 22 858.0 23 997.0 24 1149.5 25 1466.5 26 2259.0 27 3298.5 28 3658.0 29 3869.5 30 4854.0 31 5794.0 32 6905.5 33 8609.0 34 10708.5 35 11941.0 36 12907.5 37 14218.0 38 13488.0 39 11801.5 40 10346.5 41 9043.5 42 8187.0 43 7449.0 44 6709.5 45 6253.0 46 5839.0 47 6106.5 48 8683.0 49 8101.0 50 4282.0 51 2284.0 52 1334.0 53 960.5 54 797.0 55 653.0 56 508.5 57 427.0 58 401.5 59 356.5 60 307.5 61 273.0 62 230.0 63 159.0 64 101.0 65 80.5 66 70.5 67 54.5 68 40.0 69 30.0 70 24.5 71 17.0 72 10.5 73 11.5 74 10.0 75 6.5 76 5.0 77 2.5 78 1.0 79 1.5 80 1.5 81 1.5 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.239628644067023 2 0.2104167712664716 3 0.20220093204131656 4 0.20813459370392853 5 0.20493954511636822 6 0.23689003099197128 7 0.2378029020169885 8 0.2245662721542387 9 0.2250227076667473 10-11 0.23278211137939378 12-13 0.23209745811063084 14-15 0.21794795722286375 16-17 0.23164102259812222 18-19 0.2225123123479499 20-21 0.21475290863530344 22-23 0.21680686844159225 24-25 0.20904746472894578 26-27 0.21977369927289825 28-29 0.22890240952307053 30-31 0.23232567586688513 32-33 0.23004349830434206 34-35 0.23871577304200578 36-37 0.2181761749791181 38-39 0.2161222151728293 40-41 0.20767815819141994 42-43 0.2161222151728293 44-45 0.23483607118568256 46-47 0.23940042631076872 48-49 0.24236725714207466 50-51 0.24328012816709194 52-53 0.2590271533486391 54-55 0.24282369265458328 56-57 0.21269894882901466 58-59 0.1948979638411787 60-61 0.192159350766127 62-63 0.1978647946724847 64-65 0.19056182647234687 66-67 0.19330043954739856 68-69 0.1958108348661959 70-71 0.19695192364746747 72-73 0.19946231896626485 74-75 0.2028855853100795 76-77 0.21771973946660947 78-79 0.21110142453523453 80-81 0.20653706941014838 82-83 0.19969053672251913 84-85 0.19946231896626485 86-87 0.20904746472894578 88-89 0.2072217226789113 90-91 0.21749152171035516 92-93 0.20493954511636822 94-95 0.20357023857884238 96-97 0.21110142453523453 98-99 0.2181761749791181 100-101 0.1923875685223813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 219089.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.069008485136177 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52206886805784 21.3721364377034 2 9.57809286039537 5.568513252605105 3 4.0149481055788465 3.5013168164535875 4 2.361549452792564 2.745916043251829 5 1.6376968612118643 2.380311197732428 6 1.2215993844897703 2.130640972390216 7 0.8934319405844207 1.8179826463218145 8 0.6437734545511642 1.497108481028258 9 0.5950979006704665 1.5569015331668865 >10 4.682274247491638 27.780947468836864 >50 0.5793961090960479 11.534581836605215 >100 0.2590795609779076 14.013026669526996 >500 0.0031403583148837282 0.7732017581895942 >1k 0.00785089578720932 3.327414886187805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1937 0.8841155877291876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1762 0.80423937304018 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1354 0.618013683936665 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1187 0.5417889533477263 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1050 0.479257288134046 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 869 0.3966424603699867 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 825 0.37655929781960756 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 493 0.2250227076667473 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 474 0.2163504329290836 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 466 0.21269894882901466 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 457 0.20859102921643716 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 456 0.20813459370392853 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 448 0.20448310960385963 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 434 0.198093012428739 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 426 0.1944415283286701 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 424 0.19352865730365287 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 422 0.19261578627863563 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 409 0.18668212461602363 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 394 0.1798355919283944 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 378 0.17253262372825653 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 351 0.16020886489052394 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 346 0.15792668732798087 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 340 0.1551880742529292 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 333 0.15199302566536885 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 320 0.14605936400275688 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 320 0.14605936400275688 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 320 0.14605936400275688 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 306 0.13966926682763625 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 306 0.13966926682763625 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 301 0.1373870892650932 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 295 0.1346484761900415 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 281 0.12825837901492088 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 279 0.12734550798990366 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 271 0.12369402388983472 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 261 0.11912966876474858 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 254 0.11593462017718828 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 254 0.11593462017718828 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 252 0.11502174915217103 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 244 0.11137026505210212 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 243 0.11091382953959349 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 241 0.11000095851457627 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 237 0.1081752164645418 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 237 0.1081752164645418 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 231 0.10543660338949011 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 231 0.10543660338949011 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 229 0.10452373236447289 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 224 0.10224155480192981 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.2821775625430762E-4 0.0 22-23 0.0 0.0 0.0 6.846532687629229E-4 0.0 24-25 0.0 0.0 0.0 0.002282177562543076 0.0 26-27 0.0 0.0 0.0 0.006618314931374921 0.0 28-29 0.0 0.0 0.0 0.01734454947532738 0.0 30-31 0.0 0.0 0.0 0.04016632510075814 0.0 32-33 0.0 0.0 0.0 0.0750836418076672 0.0 34-35 0.0 0.0 0.0 0.1170757089584598 0.0 36-37 0.0 0.0 0.0 0.16020886489052394 0.0 38-39 0.0 0.0 0.0 0.18759499564104087 0.0 40-41 0.0 0.0 0.0 0.21749152171035513 0.0 42-43 0.0 0.0 0.0 0.24396478143585484 0.0 44-45 0.0 0.0 0.0 0.27363308974891487 0.0 46-47 0.0 0.0 0.0 0.3213306008060651 0.0 48-49 0.0 4.5643551250861523E-4 0.0 0.38158008845720237 0.0 50-51 0.0 4.5643551250861523E-4 0.0 0.428821164001844 0.0 52-53 0.0 4.5643551250861523E-4 0.0 0.5070998543970715 0.0 54-55 0.0 4.5643551250861523E-4 0.0 0.6899022771567719 0.0 56-57 0.0 4.5643551250861523E-4 0.0 1.0173947573817035 0.0 58-59 0.0 4.5643551250861523E-4 0.0 1.3031233882120965 0.0 60-61 0.0 4.5643551250861523E-4 0.0 1.5881673657737267 0.0 62-63 0.0 4.5643551250861523E-4 0.0 1.9277553870801363 0.0 64-65 0.0 4.5643551250861523E-4 0.0 2.3492735828818425 0.0 66-67 0.0 4.5643551250861523E-4 0.0 2.9615818229121498 0.0 68-69 0.0 4.5643551250861523E-4 0.0 3.6984969578573095 0.0 70-71 0.0 4.5643551250861523E-4 0.0 4.376531911688857 0.0 72-73 0.0 4.5643551250861523E-4 0.0 5.046122808538995 0.0 74-75 0.0 4.5643551250861523E-4 0.0 5.8227478330724045 0.0 76-77 0.0 4.5643551250861523E-4 0.0 6.687921347032485 0.0 78-79 0.0 4.5643551250861523E-4 0.0 7.4202721268525575 0.0 80-81 0.0 4.5643551250861523E-4 0.0 8.017746212726335 0.0 82-83 0.0 4.5643551250861523E-4 0.0 8.684142060988913 0.0 84-85 0.0 4.5643551250861523E-4 0.0 9.619834861631574 0.0 86-87 0.0 4.5643551250861523E-4 0.0 10.787396902628611 0.0 88-89 0.0 4.5643551250861523E-4 0.0 12.206683128774152 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 20 0.0020708502 71.258415 5 CCGCCAC 25 0.0016155989 37.99579 84-85 GCACATG 55 0.0025735795 34.549534 6 CCTTTAG 105 1.8838637E-6 31.68492 1 GCTGGGG 60 0.0039417283 31.670406 9 CGGGACT 30 0.00396374 31.641422 58-59 CTTTAGG 110 2.7224542E-6 30.223923 2 TACACGG 130 3.0147567E-7 29.23422 5 ACACGGA 135 4.1944077E-7 28.151472 6 ACGGAGG 135 4.1944077E-7 28.151472 8 CGGAGGT 135 4.1944077E-7 28.151472 9 CATTCCG 120 5.3506174E-6 27.711607 9 GGCTTAT 70 0.0083534345 27.1585 1 GTATAGG 140 5.740949E-7 27.1585 1 TGGGTAT 70 0.008372129 27.14606 6 CTACACG 140 5.7639954E-7 27.14606 4 AGTACCT 35 0.008381145 27.14606 12-13 TATAAGG 70 0.008381489 27.139847 2 GTTATGG 70 0.008381489 27.139847 3 TACAGCG 35 0.008399889 27.133635 78-79 >>END_MODULE