##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937312_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 219089 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29262080706928 33.0 31.0 34.0 31.0 34.0 2 32.49955953973043 34.0 31.0 34.0 31.0 34.0 3 32.516004911246114 34.0 31.0 34.0 31.0 34.0 4 36.16528442778962 37.0 35.0 37.0 35.0 37.0 5 36.10532249451136 37.0 35.0 37.0 35.0 37.0 6 36.17682311754584 37.0 36.0 37.0 35.0 37.0 7 36.12823099288417 37.0 35.0 37.0 35.0 37.0 8 36.13838668303749 37.0 35.0 37.0 35.0 37.0 9 37.83980939252998 39.0 38.0 39.0 35.0 39.0 10-11 37.78219810214114 39.0 37.0 39.0 35.0 39.0 12-13 37.72467581667724 39.0 37.0 39.0 35.0 39.0 14-15 38.861843816896325 40.0 38.0 41.0 35.0 41.0 16-17 38.83912930361633 40.0 38.0 41.0 35.5 41.0 18-19 38.92621491722542 40.0 38.0 41.0 35.5 41.0 20-21 38.90787305615526 40.0 38.0 41.0 35.0 41.0 22-23 38.776088712806214 40.0 38.0 41.0 35.0 41.0 24-25 38.689710574241516 40.0 38.0 41.0 34.5 41.0 26-27 38.586275440574376 40.0 38.0 41.0 34.5 41.0 28-29 38.47609419003236 40.0 38.0 41.0 34.0 41.0 30-31 38.45663634413412 40.0 38.0 41.0 35.0 41.0 32-33 38.287154991806986 40.0 37.0 41.0 34.0 41.0 34-35 38.112032096545235 40.0 37.0 41.0 34.0 41.0 36-37 37.897705955114134 40.0 37.0 41.0 33.5 41.0 38-39 37.7307829238346 40.0 37.0 41.0 33.0 41.0 40-41 37.547006011255704 40.0 37.0 41.0 33.0 41.0 42-43 37.266802532304226 40.0 36.5 41.0 33.0 41.0 44-45 36.86906919105934 40.0 36.0 41.0 32.5 41.0 46-47 36.32204035802802 40.0 36.0 41.0 30.5 41.0 48-49 35.793481188010354 40.0 36.0 41.0 28.5 41.0 50-51 35.50568946866342 39.5 35.5 41.0 27.0 41.0 52-53 35.303353888145914 39.0 35.0 41.0 26.5 41.0 54-55 35.08832255384798 39.0 35.0 41.0 25.5 41.0 56-57 34.829621751890784 39.0 35.0 40.0 23.5 41.0 58-59 34.61236301229181 39.0 35.0 40.0 22.5 41.0 60-61 34.28278233959715 38.5 35.0 40.0 21.0 41.0 62-63 33.95296660261355 38.0 34.0 40.0 18.5 41.0 64-65 33.589173806078804 38.0 34.0 40.0 15.0 41.0 66-67 33.15666692531346 37.0 33.5 39.5 8.0 41.0 68-69 32.6861595059542 37.0 33.0 39.0 4.5 40.5 70-71 32.26658800761334 36.0 32.5 39.0 2.0 40.0 72-73 31.792620807069273 36.0 32.0 38.5 2.0 40.0 74-75 31.297349478978862 35.0 31.5 37.5 2.0 39.5 76-77 29.964425416155077 34.0 30.0 36.0 2.0 38.5 78-79 30.464751767546524 35.0 31.0 36.0 2.0 39.0 80-81 30.211713048121997 35.0 31.0 36.0 2.0 38.5 82-83 29.833291493411352 34.5 31.0 36.0 2.0 37.0 84-85 29.45603841361273 34.0 30.5 35.0 2.0 37.0 86-87 29.106406528853572 34.0 30.0 35.0 2.0 36.5 88-89 28.601931178653423 34.0 29.0 35.0 2.0 36.0 90-91 28.142442112566126 34.0 29.0 35.0 2.0 36.0 92-93 27.874984595301456 34.0 29.0 35.0 2.0 35.0 94-95 27.46297166904774 34.0 27.5 35.0 2.0 35.0 96-97 27.007442181031454 33.5 26.5 35.0 2.0 35.0 98-99 26.3888123091529 33.0 25.0 35.0 2.0 35.0 100-101 24.11331468033539 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15099984332155003 1101 2 -0.13256411193943762 1101 3 -0.23761701389293677 1101 4 -0.07078201552565844 1101 5 -0.12990467506639902 1101 6 -0.03556836951404563 1101 7 -0.11892085749763481 1101 8 0.003447542806334525 1101 9 0.02978041035403578 1101 10-11 -0.08742706299022984 1101 12-13 -0.3173782420368454 1101 14-15 -0.10391342216909294 1101 16-17 -0.17071560713078782 1101 18-19 -0.15078792162307053 1101 20-21 -0.07048393905586892 1101 22-23 -0.30254680649709087 1101 24-25 -0.34935900257190866 1101 26-27 -0.08540151166364751 1101 28-29 -0.2116985100114377 1101 30-31 -0.26424228321292986 1101 32-33 0.1465284242126188 1101 34-35 -0.031935856710575194 1101 36-37 0.003242545012376752 1101 38-39 0.36435331364331347 1101 40-41 0.11286862602574388 1101 42-43 0.28635564658442547 1101 44-45 0.2539573332724032 1101 46-47 0.7797074578851237 1101 48-49 0.6649927226527197 1101 50-51 1.134646757057979 1101 52-53 1.0878761085811277 1101 54-55 0.8669360428978266 1101 56-57 0.9188372049148867 1101 58-59 0.973319510113086 1101 60-61 0.8355039653856764 1101 62-63 0.933780716953045 1101 64-65 0.8325041681944612 1101 66-67 1.175491754697525 1101 68-69 1.1484498153199283 1101 70-71 0.92539518842975 1101 72-73 1.1823621172500474 1101 74-75 1.189740588380925 1101 76-77 1.042282737806385 1101 78-79 0.4473857759800559 1101 80-81 0.7237963637920153 1101 82-83 0.6437944989721167 1101 84-85 0.2958431937937611 1101 86-87 0.3732994978674604 1101 88-89 -0.13814425696544674 1101 90-91 0.007013367049747643 1101 92-93 0.4234666962984903 1101 94-95 0.31760996495615856 1101 96-97 0.3371375703877426 1101 98-99 -0.05696955934342185 1101 100-101 1.2076213635365747 1102 1 -0.057563574418260544 1102 2 0.08064595287184773 1102 3 0.034749233406593305 1102 4 -0.052418818168945336 1102 5 -0.031003925288793255 1102 6 -0.10902115894091224 1102 7 -0.09588743520763643 1102 8 -0.22459922298347124 1102 9 -0.28846206009964703 1102 10-11 -0.17778161884230315 1102 12-13 -0.28967459481356883 1102 14-15 -0.2864175522999872 1102 16-17 -0.017487750986425965 1102 18-19 -0.01579618188485199 1102 20-21 -0.12415975555096992 1102 22-23 -0.2549232202726657 1102 24-25 0.07725174847650607 1102 26-27 -0.19448462260532295 1102 28-29 -0.3183511977735449 1102 30-31 0.2316752412501515 1102 32-33 0.19837745204334567 1102 34-35 0.05957831281541104 1102 36-37 -0.15265401109385834 1102 38-39 0.1912531738542711 1102 40-41 0.07186913434954079 1102 42-43 -0.32576152205199804 1102 44-45 0.026164729383729934 1102 46-47 0.23697648825746853 1102 48-49 0.08297785418150028 1102 50-51 -0.014736900331783431 1102 52-53 0.35333232919366253 1102 54-55 0.32318842566565564 1102 56-57 0.496181848452828 1102 58-59 0.7705777386046435 1102 60-61 0.7602243872944356 1102 62-63 0.6644802975859108 1102 64-65 0.36873812422444985 1102 66-67 0.3113571759708762 1102 68-69 0.5916287452983298 1102 70-71 0.7557103491871437 1102 72-73 0.7947175626687759 1102 74-75 0.8917992997800859 1102 76-77 0.9153765235479021 1102 78-79 0.15135070163769626 1102 80-81 0.23949086118685514 1102 82-83 0.521717360835126 1102 84-85 0.311633001901523 1102 86-87 0.5647310647755823 1102 88-89 0.36369841681775483 1102 90-91 0.442805716776526 1102 92-93 0.38546949207940173 1102 94-95 0.4255811175803821 1102 96-97 0.5944288399264401 1102 98-99 1.0810403529707244 1102 100-101 1.6948179577670963 1103 1 0.20449499577984653 1103 2 0.12661925175212474 1103 3 0.11173028422485487 1103 4 0.1816466426407075 1103 5 0.07827686713153525 1103 6 0.1723095904130929 1103 7 0.247351152217 1103 8 -0.09066296114023231 1103 9 0.10947075643782256 1103 10-11 0.2163828945082571 1103 12-13 0.09097785996679164 1103 14-15 0.18160570351397354 1103 16-17 0.2317585883762021 1103 18-19 0.3114540334467577 1103 20-21 0.04153791886762548 1103 22-23 0.13924129307788746 1103 24-25 0.30744124029391173 1103 26-27 0.09185258669700858 1103 28-29 0.1912310589017352 1103 30-31 0.3357880750141433 1103 32-33 0.33285204291328796 1103 34-35 0.3142617753477097 1103 36-37 0.7344583920069212 1103 38-39 0.44453698091714244 1103 40-41 0.5788674116966632 1103 42-43 0.2602096235121678 1103 44-45 0.5527581833027639 1103 46-47 1.0739166260697033 1103 48-49 1.2428615751117391 1103 50-51 1.0239151237853577 1103 52-53 0.856508470451196 1103 54-55 0.814036832211734 1103 56-57 0.7363842601668651 1103 58-59 0.707647075383278 1103 60-61 0.8370977723073523 1103 62-63 1.0423872743300961 1103 64-65 0.6487230510117001 1103 66-67 0.6009243594334137 1103 68-69 0.5735408900011763 1103 70-71 0.7080682303240984 1103 72-73 0.8213648494904895 1103 74-75 0.8042670000384824 1103 76-77 1.0630401755720236 1103 78-79 0.5805066017238296 1103 80-81 0.9988039533487836 1103 82-83 0.9238491437808634 1103 84-85 0.5104379114622475 1103 86-87 0.6821944584017672 1103 88-89 0.5681019482561709 1103 90-91 0.7668797908505951 1103 92-93 0.6971081656366813 1103 94-95 0.7934751744279254 1103 96-97 0.7988862042675215 1103 98-99 0.39574750198019615 1103 100-101 0.951170240282174 1104 1 -0.16015878843915488 1104 2 -0.02825192377122221 1104 3 0.035119978266720864 1104 4 -0.11311986299536869 1104 5 -0.12345421177345628 1104 6 -0.10534741441782103 1104 7 -0.31867139206642037 1104 8 -0.27552608513379084 1104 9 -0.09149542713705472 1104 10-11 -0.12737716990398695 1104 12-13 -0.14628059413949046 1104 14-15 -0.27310444141356527 1104 16-17 -0.11868424576228875 1104 18-19 -0.054387351416366414 1104 20-21 0.04407415075169041 1104 22-23 -0.14727239452275143 1104 24-25 -0.1387745407044818 1104 26-27 -0.08477784808560074 1104 28-29 -0.026844625478297246 1104 30-31 -0.32488020195847866 1104 32-33 -0.35293700700619013 1104 34-35 -0.3802430555701619 1104 36-37 -0.2131865355293101 1104 38-39 -0.7414734860715768 1104 40-41 -0.5237493354853058 1104 42-43 -0.5596172685973286 1104 44-45 -0.7818231371481161 1104 46-47 -0.5701856283586366 1104 48-49 -0.5038775553230437 1104 50-51 -0.7977837490004731 1104 52-53 -0.47076608671466147 1104 54-55 -0.4231209777721645 1104 56-57 -0.9592276560972195 1104 58-59 -0.7905614592382975 1104 60-61 -0.8691824209293557 1104 62-63 -0.8750040300177346 1104 64-65 -1.0103147911783168 1104 66-67 -1.0138703265569262 1104 68-69 -1.3334639409167046 1104 70-71 -1.2299148614633388 1104 72-73 -0.8958452954370522 1104 74-75 -0.8021339661450924 1104 76-77 -0.8066659435906196 1104 78-79 -0.7357574884533022 1104 80-81 -0.9655478985030648 1104 82-83 -0.9997015808568221 1104 84-85 -1.347955808344512 1104 86-87 -1.2088280858816454 1104 88-89 -0.9684721258179039 1104 90-91 -1.0557450078611552 1104 92-93 -0.8230407876644463 1104 94-95 -0.7861141975527524 1104 96-97 -1.068867418920881 1104 98-99 -0.353085026200155 1104 100-101 0.23502692305189754 1105 1 -0.2627715665571415 1105 2 -0.1323139772515276 1105 3 -0.06739073820602925 1105 4 -0.06704923633686377 1105 5 -0.022596893953497954 1105 6 -0.17960746748988043 1105 7 -0.23100043881065346 1105 8 -0.4046953033415903 1105 9 -0.08379997908643588 1105 10-11 0.012222748446703235 1105 12-13 -0.07109178009580575 1105 14-15 0.10238035140128687 1105 16-17 -0.09577140640731585 1105 18-19 -0.25815342612549586 1105 20-21 -0.28299259756429507 1105 22-23 -0.23275922857051512 1105 24-25 -0.6572170799982118 1105 26-27 -0.25527182644852786 1105 28-29 -0.34834901412904173 1105 30-31 -0.653192102346317 1105 32-33 -0.6048761782656413 1105 34-35 -0.25039002433930335 1105 36-37 -0.045764612687918316 1105 38-39 -0.13348205924977918 1105 40-41 -0.14772361595071004 1105 42-43 0.12076648318874561 1105 44-45 0.10133669138831891 1105 46-47 0.6381119833988578 1105 48-49 0.7622277722948354 1105 50-51 0.8552270695339246 1105 52-53 0.3633225027933946 1105 54-55 0.3898714696660832 1105 56-57 0.5583065345539282 1105 58-59 1.0026718536827062 1105 60-61 0.9975046580663545 1105 62-63 0.9050633306681277 1105 64-65 0.7257640004118073 1105 66-67 1.368432184159552 1105 68-69 0.8219759447742518 1105 70-71 0.7924774635009335 1105 72-73 0.6831606241383703 1105 74-75 0.3392826494907517 1105 76-77 0.4022746565318549 1105 78-79 0.29503450991370883 1105 80-81 -0.26766057455940384 1105 82-83 -0.23870954166514835 1105 84-85 -0.23594315270050714 1105 86-87 -0.522096099762031 1105 88-89 0.2942039305201227 1105 90-91 0.1564207402507023 1105 92-93 -0.3780418082809014 1105 94-95 0.2369415281055467 1105 96-97 0.1633228239858333 1105 98-99 0.1786765577965781 1105 100-101 0.8896809340745584 1106 1 -0.22654425242357945 1106 2 0.102918312006409 1106 3 -0.06122438686414711 1106 4 -0.05149321363347781 1106 5 -0.19749704110505917 1106 6 -0.09033551738126988 1106 7 -0.0036162330787448127 1106 8 -0.03317263499319267 1106 9 0.025722829406312542 1106 10-11 -0.22108255601689564 1106 12-13 -0.11103291946935911 1106 14-15 -0.12871767957060598 1106 16-17 0.1679802578068248 1106 18-19 -0.22651583941117792 1106 20-21 0.10559264523579515 1106 22-23 0.11844250921940613 1106 24-25 0.17629143377097023 1106 26-27 -0.2797270152930409 1106 28-29 -0.3020663429777599 1106 30-31 -0.2550770438360459 1106 32-33 -0.10834475089544071 1106 34-35 -0.023321143391925148 1106 36-37 -0.22294210550551696 1106 38-39 -0.4066584309125929 1106 40-41 -0.3704830581762053 1106 42-43 -0.12788462745521656 1106 44-45 0.12358460674112592 1106 46-47 0.0011208825650044218 1106 48-49 0.5469660527236826 1106 50-51 0.6549958313586046 1106 52-53 0.6239559552494285 1106 54-55 0.6213198615096829 1106 56-57 0.42716646027742655 1106 58-59 0.37780323900959445 1106 60-61 0.2914924655080213 1106 62-63 0.28741214995199016 1106 64-65 0.6738405192261894 1106 66-67 0.5730429332310862 1106 68-69 0.7008211553411385 1106 70-71 0.7796892916839688 1106 72-73 0.5418250484954648 1106 74-75 0.3231310133054812 1106 76-77 0.9972925249363094 1106 78-79 1.0156917869090165 1106 80-81 0.49241230050190765 1106 82-83 0.2466103378107114 1106 84-85 0.6875730690078505 1106 86-87 0.5823464760911001 1106 88-89 0.8805328493064728 1106 90-91 0.5771340749854801 1106 92-93 0.8859901446306004 1106 94-95 0.6282595857048747 1106 96-97 0.09552799531229894 1106 98-99 -0.21211595849520393 1106 100-101 1.0034781452020205 1107 1 -0.03503801549229735 1107 2 -0.09298622328812911 1107 3 0.08536152416044018 1107 4 0.04678351864714614 1107 5 0.06640086069030815 1107 6 -9.995883308704379E-4 1107 7 -0.1278501360277673 1107 8 0.07519855254736996 1107 9 0.17951906561656727 1107 10-11 0.04025568033756599 1107 12-13 -0.015604265637072956 1107 14-15 0.05056705617096924 1107 16-17 0.15788580254689322 1107 18-19 -0.012116820015400265 1107 20-21 0.17499202514782297 1107 22-23 0.2783314702115476 1107 24-25 -0.2936054585788739 1107 26-27 -0.25989862103246253 1107 28-29 -0.18215863514013364 1107 30-31 -0.2509671584802149 1107 32-33 -0.64641526787576 1107 34-35 -1.2766198671643707 1107 36-37 -0.7924135886694117 1107 38-39 -0.9170773508058758 1107 40-41 -1.0384836350022155 1107 42-43 -0.8852211333316262 1107 44-45 -1.1354463255539216 1107 46-47 -0.8438571259431811 1107 48-49 -1.4799645118447842 1107 50-51 -1.7866726378893603 1107 52-53 -1.8205245408209478 1107 54-55 -1.9977586589315877 1107 56-57 -2.748116544986111 1107 58-59 -2.3864551790450577 1107 60-61 -1.774013338803755 1107 62-63 -1.726936397167485 1107 64-65 -1.941957303255606 1107 66-67 -1.84382201737818 1107 68-69 -1.8745267428490706 1107 70-71 -2.0023786295792796 1107 72-73 -1.8540578558235232 1107 74-75 -2.0224238212175543 1107 76-77 -1.6119799532524546 1107 78-79 -1.7403468604339807 1107 80-81 -1.917963357440268 1107 82-83 -1.9765131750597185 1107 84-85 -1.9385355184894415 1107 86-87 -1.6561710810507293 1107 88-89 -1.784655700697133 1107 90-91 -1.5692715779094684 1107 92-93 -1.708789579934976 1107 94-95 -2.4486745840261825 1107 96-97 -2.126838433413635 1107 98-99 -1.8540512097750366 1107 100-101 -1.410142159073704 1108 1 0.06471579350064616 1108 2 0.15991697254984416 1108 3 -0.028048918054345506 1108 4 -0.12248441149034761 1108 5 0.05601903237369754 1108 6 0.06066429126779127 1108 7 -0.0412884489226073 1108 8 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56-57 -0.21220248447250611 2316 58-59 -0.2961297277356323 2316 60-61 -0.13951041482715 2316 62-63 -0.2339285151435888 2316 64-65 -0.29048260200973886 2316 66-67 -0.26493821366600656 2316 68-69 -0.9776287006421036 2316 70-71 -0.5481452199683012 2316 72-73 -0.25648602770249695 2316 74-75 -0.0020195496691179926 2316 76-77 -0.1761167445059506 2316 78-79 0.011305821996735688 2316 80-81 0.22041701377763445 2316 82-83 0.3514807227282297 2316 84-85 0.5517390810528902 2316 86-87 0.072789000312099 2316 88-89 -0.0026826521336928977 2316 90-91 0.3901741378291561 2316 92-93 0.5810019278199157 2316 94-95 3.465194571639074E-4 2316 96-97 0.19143006391664485 2316 98-99 0.44374945129793275 2316 100-101 -1.310282001433226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 2.0 6 6.0 7 28.0 8 53.0 9 110.0 10 202.0 11 359.0 12 611.0 13 934.0 14 1159.0 15 1832.0 16 2652.0 17 4106.0 18 3651.0 19 1803.0 20 1307.0 21 1213.0 22 1248.0 23 1301.0 24 1498.0 25 1616.0 26 1718.0 27 2179.0 28 2527.0 29 3220.0 30 3971.0 31 4828.0 32 6269.0 33 8367.0 34 11664.0 35 17678.0 36 27728.0 37 49209.0 38 48783.0 39 5255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.93870550782624 24.753474648652844 16.896178457406723 18.411641386114194 2 15.118513480822863 24.663036482890515 39.57569754757199 20.642752488714635 3 18.017335420765075 28.92933921830854 34.52067424653908 18.532651114387306 4 11.354746244676821 21.78429770549868 41.95737805184195 24.903577997982556 5 10.185683899179303 40.83676431655727 37.3636355337271 11.61391625053633 6 24.613285012027074 42.921369854260135 18.420367978310185 14.044977155402599 7 22.379033178297405 38.08954351884394 24.1536544509309 15.377768851927756 8 23.39232001606653 35.219477016189764 21.895667970550782 19.492534997192923 9 24.134484159405538 18.945725253207602 25.394702609441826 31.525087977945038 10-11 21.40504864472576 29.429887922004124 29.87103385161704 19.294029581653078 12-13 24.02215639107354 28.877482551956145 29.32663559720466 17.773725459765654 14-15 21.789546713892527 30.157607182469228 29.424799967136643 18.628046136501606 16-17 17.216519314068712 32.87408313516425 31.10950344380594 18.799894106961098 18-19 16.586866524563078 31.575067666564728 35.059496369055495 16.778569439816696 20-21 17.8543879427995 29.960883476578008 34.89837463313996 17.28635394748253 22-23 18.01901519024319 29.756307967574674 34.445747827357046 17.778929014825092 24-25 18.633879157870716 30.391282022023162 34.70302961145661 16.27180920864951 26-27 18.09953511936957 29.398622018042936 36.25019113280706 16.25165172978043 28-29 16.791732851326433 30.200516696792096 36.23176498511987 16.775985466761608 30-31 17.927417624800878 29.509925190219498 35.419623988424796 17.143033196554825 32-33 17.69166868259018 30.71628424977977 34.868934542583155 16.723112525046897 34-35 18.49750557992414 29.687706822341603 35.05105231207409 16.763735285660168 36-37 18.758814910835323 28.996891674159812 35.28292155242846 16.961371862576396 38-39 17.604033064188528 30.777903043968436 34.97893550109773 16.639128390745313 40-41 17.39064946209075 30.491946195381786 35.674086786648346 16.443317555879116 42-43 17.432099328981604 30.83466471903958 34.129273748117036 17.603962203861776 44-45 17.92928428466122 30.148547276557192 33.95226130079854 17.96990713798305 46-47 19.000725732464886 30.960705466728133 32.819767309175724 17.218801491631254 48-49 18.654747613983357 30.983070806841056 32.867464820232875 17.49471675894271 50-51 17.647394437876844 31.120229678349894 33.17213552483238 18.060240358940888 52-53 18.292109599295266 32.1590769048195 32.04998881732995 17.49882467855529 54-55 18.366965023346676 30.94358913500906 31.856231942270036 18.833213899374226 56-57 18.778213420116938 31.39477563912383 31.612495378590438 18.2145155621688 58-59 19.008941571690045 30.250948244777238 32.092893755505756 18.647216428026965 60-61 18.610016933757514 31.26971230869647 32.32727339117893 17.792997366367093 62-63 18.307171971208046 30.811451054137816 32.90306679020855 17.97831018444559 64-65 18.21771061075636 31.40778405123032 32.156110073988195 18.218395264025123 66-67 18.15632003432395 31.011826244129097 31.335895458010217 19.495958263536735 68-69 17.927417624800878 30.25642547092734 32.24602786995239 19.570129034319386 70-71 18.8583178525622 30.681595150829118 32.15154571886311 18.308541277745576 72-73 18.78414708177955 29.12857331951855 33.18080779957004 18.90647179913186 74-75 17.714718675971866 31.225209846226875 31.75535969400563 19.30471178379563 76-77 18.17959824546189 30.14893490773156 32.21613134388308 19.455335502923468 78-79 18.041526502928036 30.298645755834386 31.881107677701753 19.778720063535822 80-81 18.300097220764165 30.365285340660648 31.093756418624395 20.240861019950795 82-83 17.68170780690864 30.599576425911053 31.771023515664936 19.947692251515374 84-85 17.387037658297903 30.285478242810555 31.55847979241265 20.769004306478887 86-87 17.387682631259445 30.607880815558975 31.34616525704166 20.658271296139922 88-89 18.158145776373985 29.5338880546262 30.71993573387984 21.588030435119972 90-91 17.67966897407983 28.988504015720533 31.38378602908117 21.94804098111847 92-93 18.190241664745795 29.17527255465357 31.472787896907356 21.16169788369328 94-95 18.16476409130536 28.55574675132024 31.186869263176153 22.092619894198247 96-97 18.377006604621865 28.695644235904133 30.75165800199919 22.175691157474816 98-99 18.060240358940888 29.684968209266554 29.674470192478857 22.5803212393137 100-101 17.603721134051206 29.355283398684456 30.173960278080823 22.867035189183515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 509.0 1 330.0 2 161.5 3 183.5 4 174.5 5 178.0 6 293.5 7 467.0 8 641.5 9 768.0 10 706.5 11 554.0 12 501.5 13 486.5 14 464.5 15 475.0 16 516.0 17 541.5 18 565.5 19 626.5 20 702.5 21 799.5 22 938.5 23 1053.0 24 1179.5 25 1480.0 26 2178.0 27 3213.5 28 3569.5 29 3788.0 30 4744.5 31 5576.5 32 6750.5 33 8494.0 34 10491.5 35 11683.0 36 12706.5 37 13966.0 38 13452.5 39 11803.0 40 10412.0 41 9231.0 42 8077.5 43 7571.5 44 7051.0 45 6392.0 46 5832.5 47 6289.0 48 9058.5 49 8153.0 50 4281.5 51 2463.5 52 1410.0 53 984.0 54 784.5 55 619.0 56 494.5 57 418.0 58 356.5 59 327.5 60 311.0 61 271.0 62 221.5 63 164.0 64 114.5 65 81.0 66 52.5 67 44.5 68 47.5 69 35.0 70 18.0 71 11.5 72 8.5 73 10.0 74 11.5 75 8.5 76 5.5 77 2.0 78 1.5 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06663958482625781 2 0.0 3 0.0 4 0.0 5 0.0031950485875603066 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2821775625430762E-4 12-13 0.004564355125086152 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.5643551250861523E-4 24-25 6.846532687629229E-4 26-27 2.2821775625430762E-4 28-29 0.002282177562543076 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00867227473766369 44-45 2.2821775625430762E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.5643551250861523E-4 84-85 2.2821775625430762E-4 86-87 0.0 88-89 0.0 90-91 0.0057054439063576904 92-93 0.0020539598062887686 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.007302968200137844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 219089.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.71019996439803 #Duplication Level Percentage of deduplicated Percentage of total 1 72.33898863449186 20.045278402840854 2 10.03129632679954 5.559384542354933 3 4.266183495305551 3.5465039321919405 4 2.4295832647010376 2.6929695238008295 5 1.6669411958491187 2.309563693293593 6 1.2057321693295997 2.004664770937838 7 0.9108878273760501 1.7668618689208497 8 0.7198155163893922 1.5956985517301188 9 0.6358095865590513 1.585656970454929 >10 4.92011200790644 27.776383113711777 >50 0.5452149563498601 10.214113898917791 >100 0.30637456761653764 14.41697209809712 >500 0.014824575852413112 2.548279466335599 >1k 0.008235875473562838 3.937669166411824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2325 1.0612125665825305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2236 1.0205898059692635 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1773 0.8092601636777749 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.5878889401110964 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1005 0.45871769007115826 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 847 0.3866008790947971 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 816 0.37245137820703 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 592 0.2702098234051002 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 571 0.26062467764241926 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 562 0.25651675802984175 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 0.25651675802984175 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 561 0.2560603225173331 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 554 0.2528652739297728 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 518 0.2364335954794627 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 497 0.22684844971678175 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 474 0.2163504329290836 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 470 0.21452469087904916 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 466 0.21269894882901466 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 456 0.20813459370392853 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 387 0.1766405433408341 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 366 0.16705539757815316 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 343 0.156557380790455 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 340 0.1551880742529292 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 335 0.1529058966903861 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 305 0.13921283131512766 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 294 0.13419204067753288 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 290 0.1323662986274984 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 281 0.12825837901492088 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 275 0.1255197659398692 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 269 0.1227811528648175 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 268 0.12232471735230888 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 263 0.1200425397897658 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 262 0.11958610427725719 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 261 0.11912966876474858 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 261 0.11912966876474858 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 250 0.11410887812715381 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 249 0.1136524426146452 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 248 0.11319600710213656 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 246 0.11228313607711934 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 246 0.11228313607711934 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 244 0.11137026505210212 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 242 0.11045739402708488 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 242 0.11045739402708488 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 240 0.10954452300206766 No Hit GCTAGAAACCCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATC 236 0.10771878095203319 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 232 0.10589303890199872 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 225 0.10269799031443842 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 222 0.10132868377691258 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 221 0.10087224826440397 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.2821775625430762E-4 0.0 22-23 0.0 0.0 0.0 6.846532687629229E-4 0.0 24-25 0.0 0.0 0.0 0.002282177562543076 0.0 26-27 0.0 0.0 0.0 0.006618314931374921 0.0 28-29 0.0 0.0 0.0 0.017572767231581687 0.0 30-31 0.0 0.0 0.0 0.041079196125775375 0.0 32-33 0.0 0.0 0.0 0.07713760161395597 0.0 34-35 0.0 0.0 0.0 0.12027075754602012 0.0 36-37 0.0 0.0 0.0 0.16294747796557563 0.0 38-39 0.0 0.0 0.0 0.19033360871609256 0.0 40-41 0.0 0.0 0.0 0.22068657029791547 0.0 42-43 0.0 0.0 0.0 0.24761626553592375 0.0 44-45 0.0 0.0 0.0 0.2768281383364751 0.0 46-47 0.0 0.0 0.0 0.324982084906134 0.0 48-49 0.0 0.0 0.0 0.38637266133854276 0.0 50-51 0.0 0.0 0.0 0.4354394789332189 0.0 52-53 0.0 0.0 0.0 0.5144028225972094 0.0 54-55 0.0 2.2821775625430762E-4 0.0 0.6985745518944356 0.0 56-57 0.0 4.5643551250861523E-4 0.0 1.0285774274381643 0.0 58-59 0.0 4.5643551250861523E-4 0.0 1.3188704133936437 0.0 60-61 0.0 4.5643551250861523E-4 0.0 1.6050554797365453 0.0 62-63 0.0 4.5643551250861523E-4 0.0 1.9439588477741923 0.0 64-65 0.0 4.5643551250861523E-4 0.0 2.3700413987009847 0.0 66-67 0.0 4.5643551250861523E-4 0.0 2.9880550826376497 0.0 68-69 0.0 4.5643551250861523E-4 0.0 3.73455536334549 0.0 70-71 0.0 4.5643551250861523E-4 0.0 4.420349720889684 0.0 72-73 0.0 4.5643551250861523E-4 0.0 5.095417843889926 0.0 74-75 0.0 4.5643551250861523E-4 0.0 5.881856231942271 0.0 76-77 0.0 4.5643551250861523E-4 0.0 6.759125286983828 0.0 78-79 0.0 4.5643551250861523E-4 0.0 7.501061212566583 0.0 80-81 0.0 4.5643551250861523E-4 0.0 8.104697177859226 0.0 82-83 0.0 4.5643551250861523E-4 0.0 8.776113816759398 0.0 84-85 0.0 4.5643551250861523E-4 0.0 9.72344572297103 0.0 86-87 0.0 4.5643551250861523E-4 0.0 10.90743944241838 0.0 88-89 0.0 4.5643551250861523E-4 0.0 12.343613782526734 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAGTT 35 2.780574E-4 54.295105 7 GACATAT 60 1.4826055E-6 47.519066 1 GATCAGT 40 5.380042E-4 47.497375 6 GCAGATC 40 5.380042E-4 47.497375 3 TAAGACC 40 5.380042E-4 47.497375 3 CATATAG 100 3.0009687E-8 38.015255 1 GGGCAGT 50 0.0016133994 37.997902 6 TACAATC 65 1.3233283E-4 36.544785 7 AGATCAG 65 1.3251077E-4 36.536446 5 CCTTTAG 105 4.6118657E-8 36.205006 1 CATTCCG 105 4.6211426E-8 36.19674 9 GGCATTC 105 4.6211426E-8 36.19674 7 TGAGCAG 60 0.003945102 31.664915 5 TTAGGCA 125 2.1414962E-7 30.39832 4 ACAATCA 80 4.4773633E-4 29.692636 8 CAGTCCC 65 0.0058289515 29.235828 9 AAGACCC 65 0.00583546 29.229156 4 CTTTAGG 130 3.019395E-7 29.229156 2 CAGATCA 85 6.392573E-4 27.939632 4 CATGGGT 120 5.357939E-6 27.7068 4 >>END_MODULE