FastQCFastQC Report
Thu 26 May 2016
SRR937311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88833
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8941.0063827631623383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.7508470951110511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.4446545765650152No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.2116330642891718No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1300.14634201254038476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.1429648891740682No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1180.13283351907511848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.11257077887721904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATAC250.004999082557.0199851
CTTGTAC250.00501027456.987813
TAAGCAC250.00501027456.987813
ATATAGA502.7999338E-547.5166551
GGTATCA3700.043.6639561
TTCAGAC459.5870637E-442.2131924
CTTAGGA459.5870637E-442.2131922
GTATCAA6450.040.5180781
CATGGGC905.6286626E-736.9365434
TATCAAC8150.032.048362
ATCTTGA753.0494476E-431.677771
TCTTGAT600.003935590431.6598972
CAACGCA8450.030.9280035
ATCAACG8450.030.910553
TCAACGC8450.030.910554
CGGTGGT404.4766875E-429.6895392-93
AACGCAG8900.029.3642276
TCAGACA650.005805413229.241025
GTGGTCG350.00835844627.15237494-95
GCCACGT350.00838150527.13705342-43