##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937311_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88833 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04195512928754 33.0 31.0 34.0 31.0 34.0 2 32.18185809327615 33.0 31.0 34.0 31.0 34.0 3 32.21046232818885 34.0 31.0 34.0 30.0 34.0 4 35.79548140893587 37.0 35.0 37.0 35.0 37.0 5 35.80288856618599 37.0 35.0 37.0 35.0 37.0 6 35.87779316245089 37.0 36.0 37.0 35.0 37.0 7 35.82465975482084 37.0 36.0 37.0 35.0 37.0 8 35.81612688978195 37.0 36.0 37.0 35.0 37.0 9 37.47618565172852 39.0 37.0 39.0 35.0 39.0 10-11 37.39881012686727 39.0 37.0 39.0 35.0 39.0 12-13 37.273822791079894 39.0 37.0 39.0 35.0 39.0 14-15 38.431551337903706 40.0 38.0 41.0 34.0 41.0 16-17 38.39084011572276 40.0 38.0 41.0 34.5 41.0 18-19 38.38469375119607 40.0 38.0 41.0 34.0 41.0 20-21 38.309834183242714 40.0 38.0 41.0 34.0 41.0 22-23 38.3409262323686 40.0 38.0 41.0 34.0 41.0 24-25 38.307774137989256 40.0 38.0 41.0 34.0 41.0 26-27 38.20562741323607 40.0 38.0 41.0 34.0 41.0 28-29 38.05092139182511 40.0 38.0 41.0 34.0 41.0 30-31 37.72990893023989 40.0 37.5 41.0 33.5 41.0 32-33 37.40302027399727 40.0 36.5 41.0 33.0 41.0 34-35 37.33653034345345 40.0 36.0 41.0 33.0 41.0 36-37 37.15154841106346 40.0 36.0 41.0 33.0 41.0 38-39 36.95239944615177 40.0 36.0 41.0 32.5 41.0 40-41 36.65414879605552 39.5 35.5 41.0 31.5 41.0 42-43 36.27558452376932 39.0 35.0 41.0 30.5 41.0 44-45 35.828762959710915 39.0 35.0 41.0 29.5 41.0 46-47 35.1733308567762 39.0 35.0 41.0 25.0 41.0 48-49 34.65777920367431 39.0 35.0 40.5 21.5 41.0 50-51 33.600818389562434 38.0 34.0 39.5 16.0 40.5 52-53 33.61949388177817 38.0 34.0 39.5 11.0 40.5 54-55 33.83216822577195 38.5 34.5 40.0 6.5 41.0 56-57 33.55254241104094 38.0 34.0 40.0 2.0 41.0 58-59 33.24323168192001 38.0 34.0 40.0 2.0 41.0 60-61 32.88386635597132 38.0 33.5 40.0 2.0 41.0 62-63 32.50166604752738 37.5 33.0 40.0 2.0 41.0 64-65 32.0848502245787 37.0 32.5 40.0 2.0 41.0 66-67 31.628617743406167 36.5 32.0 39.5 2.0 41.0 68-69 31.119730280413812 36.0 31.0 39.0 2.0 40.5 70-71 30.68009636058672 35.5 31.0 39.0 2.0 40.0 72-73 30.198659282023574 35.0 30.0 38.5 2.0 40.0 74-75 29.702148976168765 35.0 29.5 37.5 2.0 39.5 76-77 29.255327412110365 35.0 29.0 37.0 2.0 39.0 78-79 28.69926716422951 34.0 28.5 36.5 2.0 39.0 80-81 28.14668535341596 34.0 27.5 36.0 2.0 38.5 82-83 27.66918262357457 34.0 26.0 35.5 2.0 37.0 84-85 27.182769916585052 34.0 25.5 35.0 2.0 37.0 86-87 26.660030619251856 34.0 25.0 35.0 2.0 36.0 88-89 26.138805398894554 33.0 23.5 35.0 2.0 36.0 90-91 25.58797406369255 33.0 19.5 35.0 2.0 35.5 92-93 24.919872119595198 32.0 14.5 35.0 2.0 35.0 94-95 24.155758558193465 32.0 4.5 34.5 2.0 35.0 96-97 23.284573300462668 31.0 2.0 34.0 2.0 35.0 98-99 22.10306980513998 31.0 2.0 34.0 2.0 35.0 100-101 19.25042495469026 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.20110060432908838 1101 2 -0.038369572530079665 1101 3 -0.19816395513078078 1101 4 -0.04575114892384846 1101 5 -1.3119140832578395 1101 6 -0.8349773466081984 1101 7 -0.7249236530396388 1101 8 -0.4536017418353282 1101 9 -0.9332353955895698 1101 10-11 -0.8507190015964099 1101 12-13 -0.5043361955921739 1101 14-15 -0.19230919168219174 1101 16-17 -0.35932080699781466 1101 18-19 -0.31845507833323694 1101 20-21 -0.2127675420235846 1101 22-23 -0.4193233584539726 1101 24-25 -0.584636561582478 1101 26-27 -0.28176039588552015 1101 28-29 -0.5618071777244751 1101 30-31 -0.5920256370200789 1101 32-33 -0.09945628582972432 1101 34-35 0.1892716405031365 1101 36-37 -0.08365685928320943 1101 38-39 0.10059298019938723 1101 40-41 0.8562213770131706 1101 42-43 0.9678431444587474 1101 44-45 0.9095333714560425 1101 46-47 0.7270957166663479 1101 48-49 1.1375599160783665 1101 50-51 1.6030118517280556 1101 52-53 1.7735534220370184 1101 54-55 1.794215654610376 1101 56-57 2.0879872486354074 1101 58-59 1.9577474339340526 1101 60-61 1.6117784124010441 1101 62-63 2.040220981240708 1101 64-65 1.8807336335642297 1101 66-67 2.05376530195635 1101 68-69 2.365510950721987 1101 70-71 1.349815398457082 1101 72-73 1.559592508236662 1101 74-75 2.2507989572131493 1101 76-77 2.074846426027989 1101 78-79 1.8159572934177746 1101 80-81 2.640292295894202 1101 82-83 1.7895976445432034 1101 84-85 1.63525982433395 1101 86-87 2.0416322998698 1101 88-89 1.324710537034452 1101 90-91 0.44097070606667543 1101 92-93 0.8261002046954715 1101 94-95 1.3438315648576946 1101 96-97 1.064323693865429 1101 98-99 1.6704924549447817 1101 100-101 2.5290134926837275 1102 1 0.31080415757567437 1102 2 0.2884161417556328 1102 3 0.17743128296445576 1102 4 0.38282027964758214 1102 5 0.49582401198025394 1102 6 0.6084750343441812 1102 7 0.11079063267465017 1102 8 0.3785411153075273 1102 9 0.6548598425056653 1102 10-11 0.6311262364988295 1102 12-13 0.25131856631258387 1102 14-15 0.8573931892701907 1102 16-17 1.0558577644307547 1102 18-19 0.7693425407143835 1102 20-21 0.4422919817859423 1102 22-23 0.20031949868888432 1102 24-25 1.0129824860365702 1102 26-27 0.7852038898287717 1102 28-29 1.2578356794183776 1102 30-31 1.138926743932302 1102 32-33 1.0445913332178876 1102 34-35 1.4282597357412286 1102 36-37 1.4547359978596504 1102 38-39 1.2973191706755856 1102 40-41 1.1100904246322116 1102 42-43 1.7458193349349358 1102 44-45 2.0794738476465184 1102 46-47 1.3997147642853989 1102 48-49 1.4774408684593183 1102 50-51 1.299440423156625 1102 52-53 1.5610534220370198 1102 54-55 1.9454061308008548 1102 56-57 2.0183443914925476 1102 58-59 1.9509021958388146 1102 60-61 2.463564126686755 1102 62-63 1.6690900288597632 1102 64-65 2.0060312526118516 1102 66-67 1.6466224448134916 1102 68-69 2.0771180935791342 1102 70-71 2.2914820651237484 1102 72-73 2.9211996510938008 1102 74-75 2.7874061000702923 1102 76-77 2.1549059498375165 1102 78-79 2.1397668172272972 1102 80-81 1.9530899149418204 1102 82-83 1.7497166921622522 1102 84-85 1.8373431576672843 1102 86-87 1.915144204631705 1102 88-89 2.247924822748736 1102 90-91 2.004661182257152 1102 92-93 2.91419544279071 1102 94-95 2.6482958505719765 1102 96-97 1.1253355986273306 1102 98-99 0.30948055018287945 1102 100-101 1.3421087307789676 1103 1 0.21080415757567295 1103 2 -0.12408385824436863 1103 3 0.2506455686787419 1103 4 0.3256774225047252 1103 5 0.7726097262659692 1103 6 0.3959750343441826 1103 7 0.46436206124607793 1103 8 0.5428268295932455 1103 9 0.7548598425056667 1103 10-11 0.8552333793559654 1103 12-13 0.8289971377411547 1103 14-15 0.5618574749844782 1103 16-17 0.603179193002191 1103 18-19 0.54702111214295 1103 20-21 0.005684838928800673 1103 22-23 0.4324623558317455 1103 24-25 -0.5870175139634313 1103 26-27 0.1789538898287688 1103 28-29 0.27033567941838044 1103 30-31 0.6817838867894395 1103 32-33 0.673162761789321 1103 34-35 1.7934383071698008 1103 36-37 1.1609859978596475 1103 38-39 1.967854884961291 1103 40-41 1.5502689960607867 1103 42-43 1.449390763506365 1103 44-45 2.0464381333608017 1103 46-47 2.3604290499996807 1103 48-49 1.7881551541736158 1103 50-51 2.667297566013765 1103 52-53 2.666410564894157 1103 54-55 2.945406130800855 1103 56-57 2.305844391492549 1103 58-59 3.24643791012452 1103 60-61 3.2671355552581858 1103 62-63 3.0056971717168963 1103 64-65 3.7765669668975583 1103 66-67 3.8341224448134916 1103 68-69 4.328010950721993 1103 70-71 4.166482065123748 1103 72-73 4.589949651093804 1103 74-75 5.043656100070294 1103 76-77 4.343298806980375 1103 78-79 3.9665525315130132 1103 80-81 4.396839914941822 1103 82-83 4.517573835019398 1103 84-85 4.76323601481014 1103 86-87 5.41960849034599 1103 88-89 4.901496251320168 1103 90-91 3.927875467971436 1103 92-93 3.9231240142192796 1103 94-95 4.5206172791434085 1103 96-97 3.492299884341616 1103 98-99 1.678230550182878 1103 100-101 2.142108730778965 1104 1 -0.4225291757576599 1104 2 -0.17408385824436934 1104 3 -0.1434020503688771 1104 4 -0.0671797203524207 1104 5 -0.0166759880197489 1104 6 0.5793083676775126 1104 7 -0.08920936732535267 1104 8 0.18687444864086444 1104 9 -0.09514015749433469 1104 10-11 0.2873762364988295 1104 12-13 0.24923523297925243 1104 14-15 0.8469765226035264 1104 16-17 0.42044109776409755 1104 18-19 0.40892587404771774 1104 20-21 0.9027086484526023 1104 22-23 0.52948616535555 1104 24-25 0.5463158193699087 1104 26-27 0.4289538898287688 1104 28-29 0.14533567941838044 1104 30-31 0.33059341059897207 1104 32-33 0.6195913332178904 1104 34-35 0.5178430690745657 1104 36-37 0.9734859978596475 1104 38-39 1.3160691706755827 1104 40-41 0.9871737579655502 1104 42-43 1.0666526682682687 1104 44-45 0.885723847646517 1104 46-47 1.0080480976187332 1104 48-49 0.5566075351259911 1104 50-51 0.5577737564899579 1104 52-53 -0.5431132446296445 1104 54-55 0.06207279746752192 1104 56-57 0.5141777248258776 1104 58-59 0.11965219583881037 1104 60-61 0.49273079335342373 1104 62-63 -0.19549330447357605 1104 64-65 0.44144791927851657 1104 66-67 0.6049557781468238 1104 68-69 0.7750347602457985 1104 70-71 1.2164820651237456 1104 72-73 0.6191163177604722 1104 74-75 0.3853227667369623 1104 76-77 0.7236559498375144 1104 78-79 0.6064334838939658 1104 80-81 0.7760065816084882 1104 82-83 0.32888335882891795 1104 84-85 0.5935931576672857 1104 86-87 0.11306087129837294 1104 88-89 0.09375815608207105 1104 90-91 0.7754945155904842 1104 92-93 0.27252877612404447 1104 94-95 -1.2371208160946878 1104 96-97 -1.1559144013726694 1104 98-99 -1.0259361164837877 1104 100-101 -1.1495579358876995 1105 1 -0.1335354650658367 1105 2 0.3664821794914772 1105 3 -0.23554041514875124 1105 4 0.33376367587399614 1105 5 0.4663428799047793 1105 6 0.14597503434418257 1105 7 -0.027888612608371943 1105 8 -0.23042114884341203 1105 9 0.5425956915622692 1105 10-11 -0.006963386142686545 1105 12-13 -0.08205407519685082 1105 14-15 0.06034130247774527 1105 16-17 0.19701342480811945 1105 18-19 0.8364415973181494 1105 20-21 0.32424952895574677 1105 22-23 0.364234593028506 1105 24-25 0.5969447501875109 1105 26-27 0.7001803049231086 1105 28-29 0.9708073775315853 1105 30-31 0.7700588194039995 1105 32-33 0.2611007671801602 1105 34-35 0.4637550187601036 1105 36-37 1.3131086393690836 1105 38-39 0.20050313293973687 1105 40-41 1.330255518971839 1105 42-43 1.9583193349349344 1105 44-45 1.6876106400993507 1105 46-47 2.244526085040114 1105 48-49 2.18050690619517 1105 50-51 2.661232875986819 1105 52-53 2.7075156861879606 1105 54-55 3.268519338348021 1105 56-57 3.0534859009265105 1105 58-59 2.9555012524425806 1105 60-61 3.110969787064114 1105 62-63 2.865198519425796 1105 64-65 3.301668045064684 1105 66-67 3.906056407077646 1105 68-69 3.1725190369753555 1105 70-71 2.7655386688973387 1105 72-73 2.3175439907164446 1105 74-75 1.8691277981835022 1105 76-77 1.8929955724790233 1105 78-79 1.0718422889254136 1105 80-81 2.0725474621116327 1105 82-83 2.276839333671685 1105 84-85 1.6006686293654013 1105 86-87 1.0879036385939678 1105 88-89 2.0991040680317568 1105 90-91 2.1964064652760165 1105 92-93 1.1736294050548643 1105 94-95 1.5012439637795225 1105 96-97 1.325689372212235 1105 98-99 1.4600701728243841 1105 100-101 1.6727691081374552 1106 1 0.5217797673317719 1106 2 -0.29481556556144284 1106 3 0.5472483561351922 1106 4 0.08525930403782667 1106 5 0.3784459631997663 1106 6 -0.6101225266314287 1106 7 0.2339613643819618 1106 8 0.39866306652704253 1106 9 0.06583545226176568 1106 10-11 -0.27847742203775994 1106 12-13 -0.8399924092971673 1106 14-15 -0.9113568107298065 1106 16-17 -0.08301418678875194 1106 18-19 0.03961693095828167 1106 20-21 0.43055417690789 1106 22-23 0.9305024255181564 1106 24-25 0.7971288274999822 1106 26-27 1.2917587678775462 1106 28-29 0.5172868989305783 1106 30-31 1.4718535732005975 1106 32-33 0.985444991754477 1106 34-35 0.8454853454973303 1106 36-37 0.9003152661523259 1106 38-39 0.28863014628533534 1106 40-41 0.70140140024197 1106 42-43 0.44612421298371174 1106 44-45 1.1052360427684675 1106 46-47 1.221056227700025 1106 48-49 1.2996969660202993 1106 50-51 0.4207818865712625 1106 52-53 0.4564802513053152 1106 54-55 0.7899183259228053 1106 56-57 1.0040151231998706 1106 58-59 0.7928229275461334 1106 60-61 0.5260641266867552 1106 62-63 0.5732058825182946 1106 64-65 0.9875861306606275 1106 66-67 1.5673541521305658 1106 68-69 1.6766607765059582 1106 70-71 1.6969698700017979 1106 72-73 0.5122057486547789 1106 74-75 0.9156073195824881 1106 76-77 0.7376803400814147 1106 78-79 0.6226936464955912 1106 80-81 0.5812911344540161 1106 82-83 0.5957532775281074 1106 84-85 0.7978614503502115 1106 86-87 0.9951746924365814 1106 88-89 0.690912627626787 1106 90-91 0.8708197188425189 1106 92-93 1.2251710525468091 1106 94-95 1.4319848749622217 1106 96-97 1.8544514522858684 1106 98-99 2.402315916036539 1106 100-101 2.6573526332179895 1107 1 0.35366130043281885 1107 2 9.161417556313722E-4 1107 3 0.2863598543930266 1107 4 0.3613917082190099 1107 5 0.6118954405516845 1107 6 0.03883217720132848 1107 7 -0.08920936732535267 1107 8 -0.11788745612103924 1107 9 -0.04871158606576387 1107 10-11 -0.37690947778688866 1107 12-13 -0.3227885765445535 1107 14-15 -0.22385681072980645 1107 16-17 -0.14682080699780897 1107 18-19 -0.2833360307141888 1107 20-21 0.28247055321450887 1107 22-23 0.11103378440316902 1107 24-25 0.5826253431794299 1107 26-27 -0.419260395885523 1107 28-29 0.14533567941838044 1107 30-31 0.24428388678943946 1107 32-33 0.0035199046464597927 1107 34-35 1.0612954500269467 1107 36-37 1.0092002835739322 1107 38-39 0.6017834563898745 1107 40-41 0.11276899606078672 1107 42-43 -0.19346637935078093 1107 44-45 -0.5696332952106218 1107 46-47 0.11042904999968073 1107 48-49 -0.3011305601121066 1107 50-51 -0.6452024339862348 1107 52-53 -0.7085894351058428 1107 54-55 -0.6081652977705758 1107 56-57 -0.06915560850745095 1107 58-59 -0.1553478041611882 1107 60-61 0.6510641266867552 1107 62-63 1.4074828860026187 1107 64-65 1.0979955383261348 1107 66-67 0.6466224448134916 1107 68-69 0.836939522150562 1107 70-71 0.7468392079808908 1107 72-73 1.170306793950946 1107 74-75 1.1597275286417243 1107 76-77 1.325441664123229 1107 78-79 1.4754811029415826 1107 80-81 1.0932684863703948 1107 82-83 0.9729309778765369 1107 84-85 1.1382360148101398 1107 86-87 1.133894204631705 1107 88-89 0.9104248227487375 1107 90-91 0.936804039400009 1107 92-93 1.1552668713621408 1107 94-95 0.261688707714832 1107 96-97 -0.9898429728012381 1107 98-99 0.30323055018287803 1107 100-101 -0.5096769835067505 1108 1 -0.1225291757576592 1108 2 -0.4018616360221472 1108 3 -0.7156242725911 1108 4 -0.7060686092413064 1108 5 -0.23334265468641036 1108 6 -0.2151360767669317 1108 7 -0.45032047843646694 1108 8 -0.12979221802580554 1108 9 0.1993042869501096 1108 10-11 0.03182068094326951 1108 12-13 0.22979078853480672 1108 14-15 0.005309855936864949 1108 16-17 -0.12400334668035384 1108 18-19 -0.2744074592856194 1108 20-21 -0.4834024626585034 1108 22-23 -1.4455138346444443 1108 24-25 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-0.09317462422891509 2316 34-35 -0.3803838812800464 2316 36-37 -0.4467267680977969 2316 38-39 -0.8089308293244173 2316 40-41 -1.2472234051550188 2316 42-43 -2.0576381118735796 2316 44-45 -1.6993825353322052 2316 46-47 -1.499753320820986 2316 48-49 -2.2568676421789746 2316 50-51 -2.1404531938646514 2316 52-53 -1.5775104077502178 2316 54-55 -1.1317215287736175 2316 56-57 -1.186176885103194 2316 58-59 -1.487794612671827 2316 60-61 -1.1867018307600539 2316 62-63 -0.570670609438114 2316 64-65 -0.08000598143070192 2316 66-67 -0.8108243636971473 2316 68-69 -0.3788659489740631 2316 70-71 -0.5516030412592308 2316 72-73 -0.6241460935870506 2316 74-75 -0.5680460275892791 2316 76-77 -1.1747483054816357 2316 78-79 -1.900658714687598 2316 80-81 -2.189596255270942 2316 82-83 -1.5149109674122165 2316 84-85 -1.5473642891412247 2316 86-87 -1.2450951570704234 2316 88-89 -1.5576602836342417 2316 90-91 -1.4243281794449771 2316 92-93 -0.9953790252943939 2316 94-95 -0.6725818090024909 2316 96-97 -0.358573975840752 2316 98-99 0.7965816140126627 2316 100-101 2.0915768158853467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 402.0 3 85.0 4 61.0 5 78.0 6 70.0 7 80.0 8 100.0 9 103.0 10 160.0 11 230.0 12 388.0 13 534.0 14 639.0 15 865.0 16 1303.0 17 1848.0 18 1529.0 19 924.0 20 877.0 21 888.0 22 864.0 23 951.0 24 952.0 25 998.0 26 1081.0 27 1139.0 28 1287.0 29 1479.0 30 1833.0 31 2234.0 32 3039.0 33 3868.0 34 5723.0 35 8088.0 36 12131.0 37 16776.0 38 13607.0 39 1618.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13393592651523 19.991649457778983 15.853617251768847 21.020797363936943 2 14.583521342839095 21.020214782059384 41.52603555635773 22.870228318743795 3 18.10890385461437 26.83565151668979 31.682967274696267 23.37247735399957 4 11.401915548887109 17.595297993073338 39.3736674074659 31.62911905057365 5 11.938900984849338 38.01766636959489 35.24700201933599 14.796430626219781 6 28.281734619071784 38.259741724468505 18.58343201939886 14.875091637060848 7 25.443246413425967 35.11458991247857 22.263827483533337 17.17833619056212 8 25.16636213936072 33.987503101667 21.118404728068395 19.72773003090388 9 25.749029870950274 16.690731883404027 21.62259723851638 35.93764100712932 10-11 23.32991156032849 27.680827542640557 28.11343741539572 20.87582348163523 12-13 25.113081339604975 25.692305956933208 29.111253990276698 20.083358713185113 14-15 21.68626455742605 26.307419000084597 28.850915038208836 23.155401404280518 16-17 19.245908665395937 30.088650282530484 29.194816326990964 21.470624725082615 18-19 19.114371585007582 27.407340577299355 33.52937527841527 19.948912559277783 20-21 20.14402282711058 26.348733738221313 33.86904862603125 19.638194808636857 22-23 19.763383013877846 26.6622681621582 33.82337184844332 19.750976975520633 24-25 19.393440404222684 26.955133988992152 33.67093747180366 19.980488134981503 26-27 19.08907898520895 27.24191793026836 33.88745721423053 19.781545870292156 28-29 18.83913315626533 27.790089830995925 33.84423654931682 19.526540463421924 30-31 19.072164948453608 27.357373277087234 33.61399986464843 19.956461909810734 32-33 18.680990244177522 27.812552867535107 33.34630350194552 20.160153386341847 34-35 19.25232906995105 27.64149240937493 33.0992984593174 20.006880061356615 36-37 19.152559576843696 27.627527716061216 33.28972447472002 19.930188232375066 38-39 19.137598538417294 28.29786514192915 32.73730983072256 19.82722648893099 40-41 18.995528540093716 28.20628485398682 32.96362498378884 19.834561622130625 42-43 19.03626008007669 28.386059888343766 32.30869001297017 20.268990018609372 44-45 18.926219154599178 28.33378445436911 32.19143321153065 20.548563179501063 46-47 19.376758716750032 28.56409764790138 31.374660944786868 20.68448269056172 48-49 19.76169128137459 28.47524149481484 31.014531982202975 20.748535241607595 50-51 19.52401094098074 28.462904999577027 31.18348701463497 20.82959704480726 52-53 19.34306775177797 28.805644383533828 30.767191738716026 21.084096125972174 54-55 19.31325389530195 28.433589542601606 30.9030107878576 21.350145774238843 56-57 19.304725386263673 28.26604262997632 30.98962445020864 21.43960753355137 58-59 19.622569168400457 27.675817701021106 31.096037934786896 21.60557519579154 60-61 19.10787083241707 26.80108936515723 32.12161194467468 21.969427857751015 62-63 18.864041319890838 26.34988835509845 32.56366014840878 22.222410176601933 64-65 18.775477985329193 26.157116357218975 32.82156529975925 22.24584035769259 66-67 18.650795888333175 25.945452187494716 32.94690130759125 22.456850616580866 68-69 18.51889441456071 25.283331641801144 32.77794693099676 23.419827012641385 70-71 18.311248942768536 25.30025373555117 32.95742881308148 23.431068508598816 72-73 17.943498364723133 25.140408255328744 33.22826209540995 23.687831284538174 74-75 17.86235867941019 25.007189376638756 32.70405142518819 24.426400518762865 76-77 17.802827385148053 24.741593688852298 32.989731415327874 24.46584751067177 78-79 17.871099807704237 24.752581330957362 32.90174870721983 24.47457015411857 80-81 17.525389512622862 24.304572649813633 32.64913695393516 25.520900883628343 82-83 17.747755875321396 23.34284361044702 32.929428481212504 25.979972033019084 84-85 17.65956247180875 23.236919260261615 32.99785746504285 26.10566080288678 86-87 18.06787363483105 22.81699829160065 32.65749130295051 26.45763677061779 88-89 17.326235775750263 22.35505080693365 32.39801960098794 27.92069381632814 90-91 17.5571055614135 22.883386806655878 33.25176067257974 26.307746959350876 92-93 17.020700699797562 23.91266345996605 32.64800915770539 26.418626682531 94-95 17.01993289915137 24.591051340607294 30.725986072344863 27.663029687896472 96-97 17.558857529534496 25.21216905856148 30.079228578678773 27.149744833225252 98-99 18.23779851693123 24.829277920320298 29.466828319282712 27.466095243465755 100-101 19.61342633053853 23.44138525990539 29.337535874780805 27.60765253477528 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 126.0 1 73.5 2 24.0 3 27.5 4 26.5 5 32.0 6 50.5 7 89.0 8 179.5 9 225.0 10 187.0 11 168.0 12 178.0 13 203.0 14 222.5 15 253.0 16 267.5 17 292.5 18 326.5 19 328.5 20 372.5 21 430.5 22 473.0 23 524.5 24 581.5 25 675.5 26 821.5 27 974.5 28 1073.0 29 1161.5 30 1346.5 31 1587.5 32 1751.5 33 1867.0 34 2092.0 35 2343.0 36 2503.5 37 2647.0 38 2742.5 39 2806.5 40 2867.0 41 3027.5 42 3206.0 43 3340.5 44 3449.5 45 3411.5 46 3395.5 47 3512.0 48 3668.5 49 3559.0 50 3213.0 51 2940.5 52 2698.5 53 2487.5 54 2293.5 55 2001.5 56 1667.5 57 1373.0 58 1158.5 59 931.5 60 701.5 61 532.0 62 391.0 63 292.0 64 205.0 65 139.0 66 101.0 67 78.5 68 56.0 69 29.0 70 20.0 71 18.0 72 18.0 73 16.0 74 9.0 75 5.0 76 7.5 77 6.5 78 1.5 79 0.5 80 2.0 81 4.5 82 3.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24315288237479316 2 0.20825594092285524 3 0.20938164871162745 4 0.2138844798667162 5 0.2138844798667162 6 0.189118908513728 7 0.1902446163025002 8 0.19249603188004458 9 0.20825594092285524 10-11 0.20825594092285524 12-13 0.2026274019789943 14-15 0.19756171692951943 16-17 0.1913703240912724 18-19 0.18180180788670874 20-21 0.18743034683056972 22-23 0.18743034683056972 24-25 0.1902446163025002 26-27 0.18517893125302534 28-29 0.18743034683056972 30-31 0.19699886303513334 32-33 0.189118908513728 34-35 0.19249603188004458 36-37 0.18686749293618363 38-39 0.18236466178109487 40-41 0.1795503923091644 42-43 0.189118908513728 44-45 0.18574178514741144 46-47 0.1885560546193419 48-49 0.18743034683056972 50-51 0.19756171692951943 52-53 0.20093884029583603 54-55 0.1896817624081141 56-57 0.18349036956986706 58-59 0.17392185336530344 60-61 0.17729897673162 62-63 0.17898753841477827 64-65 0.17279614557653125 66-67 0.1784246845203922 68-69 0.17561041504846173 70-71 0.1778618306260061 72-73 0.18349036956986706 74-75 0.18067610009793658 76-77 0.18517893125302534 78-79 0.1885560546193419 80-81 0.18517893125302534 82-83 0.17673612283723392 84-85 0.17223329168214516 86-87 0.17279614557653125 88-89 0.18461607735863925 90-91 0.1795503923091644 92-93 0.18517893125302534 94-95 0.18067610009793658 96-97 0.18630463904179753 98-99 0.18630463904179753 100-101 0.1761732689428478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 88833.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.98454403206016 #Duplication Level Percentage of deduplicated Percentage of total 1 91.56253040686117 74.15149775421295 2 5.257085667421915 8.51485371427285 3 1.3789077160450924 3.3501063793860393 4 0.5810316787367426 1.8821834228271026 5 0.38086765543987433 1.542219670617901 6 0.20572413505511464 0.9996285164297052 7 0.14456290571440486 0.8195152702261547 8 0.10425209546711887 0.6754246732633142 9 0.07784156461544878 0.567356725541184 >10 0.28495572761012494 3.9523600463791606 >50 0.012510251456054267 0.70919590692648 >100 0.006950139697807926 1.0784280616437585 >500 0.0027800558791231706 1.7572298582733892 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 1.0063827631623383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 667 0.7508470951110511 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.4446545765650152 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 188 0.2116330642891718 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 130 0.14634201254038476 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.1429648891740682 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 118 0.13283351907511848 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.11257077887721904 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0011257077887721905 0.0 20-21 0.0 0.0 0.0 0.0011257077887721905 0.0 22-23 0.0 0.0 0.0 0.0011257077887721905 0.0 24-25 0.0 0.0 0.0 0.002251415577544381 0.0 26-27 0.0 0.0 0.0 0.005065685049474857 0.0 28-29 0.0 0.0 0.0 0.009005662310177524 0.0 30-31 0.0 0.0 0.0 0.02870554861369086 0.0 32-33 0.0 0.0 0.0 0.041651188184571045 0.0 34-35 0.0 0.0 0.0 0.04896828881159029 0.0 36-37 0.0 0.0 0.0 0.06078822059369829 0.0 38-39 0.0 0.0 0.0 0.09849943151756668 0.0 40-41 0.0 0.0 0.0 0.17786183062600608 0.0 42-43 0.0 0.0 0.0 0.37373498587236725 0.0 44-45 0.0 0.0 0.0 0.5741109722738171 0.0 46-47 0.0 0.0 0.0 1.0407168507198903 0.0 48-49 0.0 0.0 0.0 1.506759875271577 0.0 50-51 0.0 0.0 0.0 1.8568549975797284 0.0 52-53 0.0 0.0 0.0 2.272241171636667 0.0 54-55 0.0 0.0 0.0 2.7050758164195736 0.0 56-57 0.0 0.0 0.0 3.8003894948949153 0.0 58-59 0.0 0.0 0.0 5.0910134747222315 0.0 60-61 0.0 0.0 0.0 6.476196908806411 0.0 62-63 0.0 0.0 0.0 8.088210462328188 0.0 64-65 0.0 0.0 0.0 9.752569428027872 0.0 66-67 0.0 0.0 0.0 11.687098263032883 0.0 68-69 0.0 0.0 0.0 13.686355295892293 0.0 70-71 0.0 0.0 0.0 15.725574955253116 0.0 72-73 0.0 0.0 0.0 17.797440140488334 0.0 74-75 0.0 0.0 0.0 19.874371010773025 0.0 76-77 0.0 0.0 0.0 22.728040255310525 0.0 78-79 0.0 0.0 0.0 24.887710648069973 0.0 80-81 0.0 0.0 0.0 27.466707192147062 0.0 82-83 0.0 0.0 0.0 30.645143133745343 0.0 84-85 0.0 0.0 0.0 34.512512242072205 0.0 86-87 0.0 0.0 0.0 37.330158837369 0.0 88-89 0.0 0.0 0.0 40.40896963966094 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGATAC 25 0.0049990825 57.019985 1 CTTGTAC 25 0.005010274 56.98781 3 TAAGCAC 25 0.005010274 56.98781 3 ATATAGA 50 2.7999338E-5 47.516655 1 GGTATCA 370 0.0 43.663956 1 TTCAGAC 45 9.5870637E-4 42.213192 4 CTTAGGA 45 9.5870637E-4 42.213192 2 GTATCAA 645 0.0 40.518078 1 CATGGGC 90 5.6286626E-7 36.936543 4 TATCAAC 815 0.0 32.04836 2 ATCTTGA 75 3.0494476E-4 31.67777 1 TCTTGAT 60 0.0039355904 31.659897 2 CAACGCA 845 0.0 30.928003 5 ATCAACG 845 0.0 30.91055 3 TCAACGC 845 0.0 30.91055 4 CGGTGGT 40 4.4766875E-4 29.68953 92-93 AACGCAG 890 0.0 29.364227 6 TCAGACA 65 0.0058054132 29.24102 5 GTGGTCG 35 0.008358446 27.152374 94-95 GCCACGT 35 0.008381505 27.137053 42-43 >>END_MODULE