FastQCFastQC Report
Thu 26 May 2016
SRR937311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88833
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.7789897898303558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.696813121249986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.3928720182814945No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2530.2848040705593642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.2138844798667162No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.2116330642891718No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.10356511656704151No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC890.10018799320072495No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA890.10018799320072495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG255.2485655E-576.02
CTTGGTA200.002067760671.252
TAAGGGT301.2946523E-463.333333
TCCGTTC250.005006071657.08
AGCAGTA250.005006071657.05
AAGGGTA352.7739216E-454.2857174
GCTAAGG352.7739216E-454.2857171
AGGGTAG451.5045658E-552.7777755
GGGTAGT459.5770956E-442.222226
ACAGGTG459.5770956E-442.222225
TAGTTTA500.001607797538.09
CATGGGC651.3183383E-436.5384644
GGTAGTT550.002566881234.5454527
ATGGGAC753.0557942E-431.6666685
ATGGGAG1201.4794205E-731.6666645
GGTGTGC600.003931520531.6666648
GATCAGG600.003931520531.6666641
TTGGTAC300.003942042631.66666446-47
AGTAGAT600.003931520531.6666642
GGTATCA6200.031.4112911