##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937311_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88833 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41587022840611 33.0 31.0 34.0 31.0 34.0 2 32.616077358639245 34.0 31.0 34.0 31.0 34.0 3 32.6538898832641 34.0 31.0 34.0 31.0 34.0 4 36.260061013362154 37.0 35.0 37.0 35.0 37.0 5 36.22148300744093 37.0 35.0 37.0 35.0 37.0 6 36.299539585514395 37.0 37.0 37.0 35.0 37.0 7 36.24122792205599 37.0 37.0 37.0 35.0 37.0 8 36.22619972307589 37.0 37.0 37.0 35.0 37.0 9 37.94879155268875 39.0 38.0 39.0 35.0 39.0 10-11 37.89508403408644 39.0 38.0 39.0 35.0 39.0 12-13 37.800760978465206 39.0 37.5 39.0 35.0 39.0 14-15 39.03390069005887 40.0 38.0 41.0 36.0 41.0 16-17 39.001238278567655 40.0 38.0 41.0 36.0 41.0 18-19 39.030512309614664 40.0 38.0 41.0 36.0 41.0 20-21 38.989992457757815 40.0 38.0 41.0 36.0 41.0 22-23 38.93214233449281 40.0 38.0 41.0 35.0 41.0 24-25 38.942949129265024 40.0 38.0 41.0 35.0 41.0 26-27 38.789413843954385 40.0 38.0 41.0 35.0 41.0 28-29 38.61803609019171 40.0 38.0 41.0 35.0 41.0 30-31 38.59055193452883 40.0 38.0 41.0 35.0 41.0 32-33 38.4041910100976 40.0 38.0 41.0 35.0 41.0 34-35 38.23908344871838 40.0 38.0 41.0 34.0 41.0 36-37 38.05416905879572 40.0 37.5 41.0 34.0 41.0 38-39 37.868100818389564 40.0 37.0 41.0 34.0 41.0 40-41 37.58772640797902 40.0 37.0 41.0 33.0 41.0 42-43 37.28081343644817 40.0 37.0 41.0 33.0 41.0 44-45 36.79015118255603 40.0 36.0 41.0 31.5 41.0 46-47 36.20758051624959 40.0 36.0 41.0 30.5 41.0 48-49 35.68643972397645 40.0 35.5 41.0 28.0 41.0 50-51 35.3008116353157 39.0 35.0 41.0 26.0 41.0 52-53 35.00732835770491 39.0 35.0 41.0 25.0 41.0 54-55 34.74835365235892 39.0 35.0 40.0 23.5 41.0 56-57 34.42989654745421 38.5 35.0 40.0 21.5 41.0 58-59 34.09276957887272 38.0 34.5 40.0 19.0 41.0 60-61 33.711120867245285 38.0 34.0 40.0 15.0 41.0 62-63 33.304261929688295 37.5 34.0 40.0 7.5 41.0 64-65 32.88968063670033 37.0 33.0 40.0 2.0 41.0 66-67 32.42689090765819 36.5 33.0 39.0 2.0 41.0 68-69 31.924656377697474 36.0 32.0 39.0 2.0 40.0 70-71 31.45453829095044 35.5 32.0 39.0 2.0 40.0 72-73 30.985585311764773 35.0 31.0 38.0 2.0 40.0 74-75 30.520639852307138 35.0 31.0 37.0 2.0 39.5 76-77 29.254550673736112 34.0 29.5 36.0 2.0 38.5 78-79 29.83191494151948 34.5 30.5 36.0 2.0 39.0 80-81 29.626518298380105 34.5 30.5 36.0 2.0 38.0 82-83 29.28420181689237 34.0 30.0 35.5 2.0 37.0 84-85 28.91328672903088 34.0 30.0 35.0 2.0 37.0 86-87 28.589685139531483 34.0 29.0 35.0 2.0 36.0 88-89 28.216220323528418 34.0 29.0 35.0 2.0 36.0 90-91 27.775950378800673 34.0 28.5 35.0 2.0 35.5 92-93 27.373982641585897 34.0 27.0 35.0 2.0 35.0 94-95 27.020037598640144 33.5 26.5 35.0 2.0 35.0 96-97 26.444277464455777 33.0 25.0 35.0 2.0 35.0 98-99 25.61586910269832 33.0 22.5 34.5 2.0 35.0 100-101 23.037953238098453 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.11387257455002242 1101 2 -0.01806156676011028 1101 3 -0.05410926314048936 1101 4 -0.09899363979995712 1101 5 -0.1567989008766304 1101 6 -0.07646419059692988 1101 7 -0.12603213130773128 1101 8 -0.2973618718555002 1101 9 -0.30004304303606233 1101 10-11 -0.23366144022502766 1101 12-13 -0.0695152549953022 1101 14-15 0.16406657545203984 1101 16-17 0.13375737127719134 1101 18-19 -0.06480774666980693 1101 20-21 -0.22178418529597366 1101 22-23 -0.28774369758024676 1101 24-25 0.03268465116864405 1101 26-27 -0.22318043368934326 1101 28-29 -0.4342383269694494 1101 30-31 0.3563048099560433 1101 32-33 0.45777523006464804 1101 34-35 0.5611039593900173 1101 36-37 0.45913936046422066 1101 38-39 0.15950859678207507 1101 40-41 0.4413139032833726 1101 42-43 0.5773427636983115 1101 44-45 0.7760932697606293 1101 46-47 0.36343228759830026 1101 48-49 0.5399115703732207 1101 50-51 1.3160515911042978 1101 52-53 1.148703438465489 1101 54-55 1.0908129960386574 1101 56-57 1.1892485566553077 1101 58-59 0.642692275636513 1101 60-61 0.5495736199906816 1101 62-63 0.7804090724581698 1101 64-65 0.44612894407644177 1101 66-67 0.3425304970210199 1101 68-69 0.430747467677282 1101 70-71 0.6114694738329831 1101 72-73 0.7841271289409697 1101 74-75 0.952135962990706 1101 76-77 0.7521096902291404 1101 78-79 1.1453208057799529 1101 80-81 0.9487192780130158 1101 82-83 0.9389745591379253 1101 84-85 1.3050020088862162 1101 86-87 1.5926321837048363 1101 88-89 1.2615823153232135 1101 90-91 1.2187199325640847 1101 92-93 0.8224001010050479 1101 94-95 0.7256980244109776 1101 96-97 1.0509503897795192 1101 98-99 1.624534258940031 1101 100-101 1.973734972806266 1102 1 0.21053218735473678 1102 2 0.09503367133512342 1102 3 0.13993835590712678 1102 4 0.21469683639051595 1102 5 -0.31096556754329896 1102 6 -0.010988000120740082 1102 7 -0.17484165511725536 1102 8 0.06335241385878732 1102 9 -0.41968590017891927 1102 10-11 -0.03604239260597808 1102 12-13 -0.20284858832863506 1102 14-15 -0.03265961502414427 1102 16-17 0.6289954665152848 1102 18-19 0.1691208247587639 1102 20-21 0.20381105279926715 1102 22-23 0.1164229690864147 1102 24-25 -0.4589820154980231 1102 26-27 -0.4705018622607824 1102 28-29 -0.5919764222075514 1102 30-31 -0.3338737614725247 1102 32-33 -0.06276048422106584 1102 34-35 0.1962230070090598 1102 36-37 0.22967507474993454 1102 38-39 0.24730621582969547 1102 40-41 0.454409141378612 1102 42-43 0.18716419226974068 1102 44-45 0.5740099364272879 1102 46-47 0.07265847807448722 1102 48-49 0.19229252275417252 1102 50-51 -0.1526984088957022 1102 52-53 0.09513200989405846 1102 54-55 0.1780153769910342 1102 56-57 0.21097474713149467 1102 58-59 -0.9013553434111046 1102 60-61 -0.764712094295021 1102 62-63 -0.25381711801802 1102 64-65 -0.3211329606854676 1102 66-67 -0.07740997916945602 1102 68-69 0.5170569914868004 1102 70-71 -0.2557924309289241 1102 72-73 0.12400808132192154 1102 74-75 0.28011215346689156 1102 76-77 0.6181811188005675 1102 78-79 0.9682374724466243 1102 80-81 1.1252073732511079 1102 82-83 1.4961174162807822 1102 84-85 1.573454389838595 1102 86-87 1.5057274218000707 1102 88-89 1.5758680296089302 1102 90-91 1.8458032658974162 1102 92-93 1.6241858152907618 1102 94-95 1.3236146910776405 1102 96-97 0.5801170564461877 1102 98-99 0.8310818779876499 1102 100-101 1.822246877568169 1103 1 0.4158893302118827 1103 2 0.34860509990655686 1103 3 0.1506526416214129 1103 4 -0.38351744932376874 1103 5 0.008677289599553717 1103 6 0.30329771416497664 1103 7 0.1073012020255959 1103 8 -0.40807615756978066 1103 9 -0.1536144716074901 1103 10-11 0.2746718931083052 1103 12-13 0.18822284024279412 1103 14-15 0.23251895640441944 1103 16-17 0.2254240379438599 1103 18-19 0.6137636819016237 1103 20-21 0.42702533851355184 1103 22-23 0.8557086833721215 1103 24-25 0.2803036987876908 1103 26-27 0.3982481377392233 1103 28-29 0.6383807206495931 1103 30-31 0.7705905242417614 1103 32-33 0.7952752300646395 1103 34-35 0.7837230070090655 1103 36-37 0.35913936046421924 1103 38-39 0.8053419301154108 1103 40-41 1.5972662842357508 1103 42-43 1.3076999065554489 1103 44-45 1.1927599364272936 1103 46-47 2.384265620931629 1103 48-49 2.6637210941827405 1103 50-51 1.9892658768185854 1103 52-53 2.6254891527512 1103 54-55 3.1110510912767495 1103 56-57 3.2672247471315004 1103 58-59 3.191501799446037 1103 60-61 2.5049307628478346 1103 62-63 3.2185043105534135 1103 64-65 2.759224182171671 1103 66-67 2.407411449401973 1103 68-69 2.708128420058234 1103 70-71 2.972778997642507 1103 72-73 3.206150938464784 1103 74-75 2.989040724895464 1103 76-77 3.1110382616577077 1103 78-79 3.2655589010180535 1103 80-81 3.2832430875368246 1103 82-83 3.2336174162807794 1103 84-85 3.996668675552879 1103 86-87 3.591441707514356 1103 88-89 3.0901537438946427 1103 90-91 3.162767551611701 1103 92-93 3.4224001010050458 1103 94-95 3.403078976791928 1103 96-97 4.205117056446188 1103 98-99 3.922153306559082 1103 100-101 3.6981397347110274 1104 1 0.0813655206880668 1104 2 0.0033670046684619592 1104 3 0.33160502257379676 1104 4 -0.07280316360948547 1104 5 0.3432010991233696 1104 6 -0.11932133345407436 1104 7 -0.13317498845059106 1104 8 0.055019080525454456 1104 9 -0.007185900178917848 1104 10-11 0.08062427406068906 1104 12-13 0.03881807833803208 1104 14-15 0.24025705164251576 1104 16-17 -0.012671200151380901 1104 18-19 0.23995415809209675 1104 20-21 0.024644386132600005 1104 22-23 -0.054410364246919585 1104 24-25 0.09935131783530693 1104 26-27 -0.1434185289274481 1104 28-29 -0.4461430888742086 1104 30-31 -0.45679042813918613 1104 32-33 -0.7231771508877358 1104 34-35 -0.9079436596575974 1104 36-37 -0.518241591916734 1104 38-39 -0.07144378417030595 1104 40-41 -0.3372575252880523 1104 42-43 0.2725808589364078 1104 44-45 0.2344266030939579 1104 46-47 -1.2877581885921785 1104 48-49 -1.6077074772458317 1104 50-51 -1.4172817422290365 1104 52-53 -1.9465346567726058 1104 54-55 -0.8615679563422987 1104 56-57 -1.3119419195351654 1104 58-59 -1.8513553434111003 1104 60-61 -1.8147120942950252 1104 62-63 -1.2600671180180179 1104 64-65 -1.6502996273521333 1104 66-67 -1.483659979169456 1104 68-69 -0.5871096751798639 1104 70-71 -1.2849590975955891 1104 72-73 -1.5384919186780763 1104 74-75 -0.6907211798664399 1104 76-77 -0.41931888119943395 1104 78-79 -0.5296791942200443 1104 80-81 -0.19770929341555643 1104 82-83 -0.27054925038588706 1104 84-85 -0.7723789434947399 1104 86-87 -0.6776059115332629 1104 88-89 -0.25746530372440546 1104 90-91 0.5270532658974147 1104 92-93 0.2366858152907625 1104 94-95 -0.6493019755890224 1104 96-97 0.23428372311285628 1104 98-99 0.4685818779876527 1104 100-101 -0.029836455765160252 1105 1 0.06312652697737775 1105 2 0.11814687888229258 1105 3 -0.03128805918721156 1105 4 0.06021570431504131 1105 5 -0.20459764301499916 1105 6 -0.229384226535835 1105 7 -0.19323788153235455 1105 8 -0.07957211444309564 1105 9 0.15602164699089371 1105 10-11 -0.13132541147390242 1105 12-13 -0.095301418517316 1105 14-15 -0.07357942634490655 1105 16-17 0.16107093821340612 1105 18-19 -0.3896055903355773 1105 20-21 0.04461293959171542 1105 22-23 -0.10032231393245894 1105 24-25 -0.22643484568670402 1105 26-27 -0.4177895981098345 1105 28-29 0.2950518796792494 1105 30-31 0.3811026536218165 1105 32-33 0.3982064969110084 1105 34-35 -0.015805294877729636 1105 36-37 0.3564439426744599 1105 38-39 -0.4572928407740804 1105 40-41 0.40959782062389394 1105 42-43 0.604027399816907 1105 44-45 0.895590125106537 1105 46-47 1.3652292327914708 1105 48-49 1.0734245982258699 1105 50-51 1.865108194877891 1105 52-53 0.8729621985733047 1105 54-55 1.2151616034061234 1105 56-57 1.051422860339052 1105 58-59 1.4708144679096549 1105 60-61 2.0050992264596914 1105 62-63 2.318234768774431 1105 64-65 1.5243859072390578 1105 66-67 1.2135098321512992 1105 68-69 1.0130475575245406 1105 70-71 0.3991603992597561 1105 72-73 1.0648099681143748 1105 74-75 1.3539329081838751 1105 76-77 0.12973772257415206 1105 78-79 0.9101006799937927 1105 80-81 1.0288630336284683 1105 82-83 0.5508343974128564 1105 84-85 0.06708646531029316 1105 86-87 0.8269538368944112 1105 88-89 1.2626604824391165 1105 90-91 0.15771364325590653 1105 92-93 -0.15576701489791844 1105 94-95 -0.9415975730732988 1105 96-97 1.1874283772009058 1105 98-99 1.4322611232706706 1105 100-101 1.3917044247379806 1106 1 -0.47391903215745756 1106 2 -0.11655169451853453 1106 3 -0.1716470099465326 1106 4 -0.8325592611704593 1106 5 -0.46736800656768906 1106 6 -0.6518416586573252 1106 7 -0.10350019170262215 1106 8 -0.45555002516559995 1106 9 0.018423855918641152 1106 10-11 -0.8982375145572021 1106 12-13 -0.8150437102798591 1106 14-15 -0.5751901028290263 1106 16-17 -0.20271185055788266 1106 18-19 -0.273866980119287 1106 20-21 -0.215396264273906 1106 22-23 -1.177174591889198 1106 24-25 -1.1778844545224132 1106 26-27 -0.9127274720168757 1106 28-29 -1.0563056905002242 1106 30-31 -0.8078066883017954 1106 32-33 -1.2101690208064397 1106 34-35 -1.4449355295763056 1106 36-37 -0.9143798032988428 1106 38-39 -0.9897364670971314 1106 40-41 -0.5913225659384622 1106 42-43 -1.2282321491936727 1106 44-45 -0.09382542942636718 1106 46-47 0.04171335612326743 1106 48-49 0.9947315471444185 1106 50-51 -0.1302898723103283 1106 52-53 -0.39694116083764186 1106 54-55 -1.0940882815455524 1106 56-57 -0.3592996431124007 1106 58-59 -0.7348919287769533 1106 60-61 -0.8110535577096627 1106 62-63 -0.9295793131399677 1106 64-65 -0.6122914972708351 1106 66-67 -0.04097705234018889 1106 68-69 0.37406918660875377 1106 70-71 0.7042685446808328 1106 72-73 1.097483691078022 1106 74-75 0.8962706900522583 1106 76-77 0.47580307002008126 1106 78-79 0.1875972285441847 1106 80-81 -0.3853109194318165 1106 82-83 -0.05754112030458458 1106 84-85 -0.5116065857711618 1106 86-87 0.45511766570250956 1106 88-89 0.6685509564381924 1106 90-91 0.11790692443400275 1106 92-93 0.014734595778563175 1106 94-95 0.24846225205325467 1106 96-97 0.7981048613242372 1106 98-99 0.6094355365242379 1106 100-101 0.449228584885244 1107 1 0.09446075878330618 1107 2 0.027176528477980355 1107 3 0.1149383559071282 1107 4 -0.09780316360948405 1107 5 -0.4734655675433004 1107 6 -0.19670228583502336 1107 7 0.1965869163113112 1107 8 0.41335241385878874 1107 9 0.1499569569639334 1107 10-11 0.2389576073940205 1107 12-13 -0.08856287404292118 1107 14-15 0.14323324211871125 1107 16-17 0.2968526093724293 1107 18-19 0.47090653904447777 1107 20-21 0.31095390994212835 1107 22-23 -0.447862745199302 1107 24-25 0.3695894130734061 1107 26-27 0.0678909948820774 1107 28-29 -0.15626213649325393 1107 30-31 0.002733381384615541 1107 32-33 0.4292038014932231 1107 34-35 0.0605087212947808 1107 36-37 -0.02478921096435016 1107 38-39 -0.18572949845601983 1107 40-41 -0.1259480014785268 1107 42-43 0.4505570494126019 1107 44-45 -0.03938292071556759 1107 46-47 0.9021227637887819 1107 48-49 1.3690782370398864 1107 50-51 1.71248016253287 1107 52-53 1.411203438465492 1107 54-55 0.6914082341338954 1107 56-57 0.47258188998863915 1107 58-59 0.2897160851603289 1107 60-61 1.1745736199906816 1107 62-63 0.8702900248391217 1107 64-65 1.1967241821716712 1107 66-67 0.8895543065448273 1107 68-69 1.4491998486296644 1107 70-71 0.9102789976425072 1107 72-73 1.3847223670362077 1107 74-75 1.1586835820383214 1107 76-77 0.9592525473719995 1107 78-79 0.6137731867323382 1107 80-81 1.104671658965394 1107 82-83 0.903260273423637 1107 84-85 0.9073829612671638 1107 86-87 1.037870278942929 1107 88-89 1.786582315323212 1107 90-91 1.1627675516117009 1107 92-93 1.3688286724336187 1107 94-95 0.9120075482204975 1107 96-97 1.1336884850176183 1107 98-99 1.3328675922733666 1107 100-101 1.7070683061396004 1108 1 0.25914329846585105 1108 2 0.2255892268906834 1108 3 0.33716057812934963 1108 4 0.27719683639051595 1108 5 0.19320109912336392 1108 6 0.4084564443237042 1108 7 0.22238056710496323 1108 8 0.34390796941434587 1108 9 0.4039252109321936 1108 10-11 0.1556242740606848 1108 12-13 0.4332625227824778 1108 14-15 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1.069086449259153 2316 58-59 1.0725808268016621 2316 60-61 1.3086921610241262 2316 62-63 1.1835499032585801 2316 64-65 1.1137074648464491 2316 66-67 1.0014464038092648 2316 68-69 1.4790250765931852 2316 70-71 1.1067075690710766 2316 72-73 1.2439548898325619 2316 74-75 0.989420664105193 2316 76-77 1.1423832464601453 2316 78-79 1.1215619405317305 2316 80-81 0.566297798783026 2316 82-83 1.1259046503233314 2316 84-85 0.9902097089875319 2316 86-87 0.7498763579702832 2316 88-89 0.6524637742897816 2316 90-91 0.0020532658974161677 2316 92-93 0.4026432620992715 2316 94-95 -0.018273606794696917 2316 96-97 0.28889365219086827 2316 98-99 0.8765606013919083 2316 100-101 0.9008373031000865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 7.0 7 15.0 8 15.0 9 43.0 10 78.0 11 141.0 12 239.0 13 326.0 14 508.0 15 779.0 16 1197.0 17 1710.0 18 1432.0 19 856.0 20 711.0 21 660.0 22 657.0 23 700.0 24 726.0 25 810.0 26 867.0 27 867.0 28 994.0 29 1212.0 30 1353.0 31 1847.0 32 2366.0 33 3323.0 34 4893.0 35 7607.0 36 12776.0 37 20585.0 38 17092.0 39 1440.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.442364443243 21.053817215201278 15.63042058074835 19.87339776080737 2 15.571915842085712 22.314905496831134 39.93786093005978 22.17531773102338 3 19.310391408598157 26.718674366507944 31.447772787139915 22.523161437753988 4 12.448076728242881 18.8432226762577 38.28194477277588 30.42675582272354 5 12.686726779462587 38.94723807594025 34.06054056488017 14.305494579716996 6 29.32468789751556 38.73110218049599 17.258226109666452 14.685983812321998 7 26.547566782614567 35.86617585807076 20.75242308601533 16.833834273299335 8 26.45075591278016 35.176116983553406 19.65148086859613 18.7216462350703 9 26.929181723008345 17.100627019238345 20.82334267670798 35.14684858104533 10-11 24.196526065763848 28.367273423164814 26.92524174574764 20.510958765323696 12-13 25.99770345270126 26.29602944984183 27.86477389140934 19.841493206047573 14-15 22.507964382605564 27.287719653732285 27.430121689011965 22.774194274650185 16-17 20.284128645886103 30.882104623281887 27.878716242837687 20.955050487994324 18-19 20.086004075062196 28.324496527191474 32.176668580369906 19.412830817376424 20-21 21.34341967512073 27.071020904393638 32.33257910911485 19.252980311370774 22-23 20.866026499757968 27.65588589568957 32.08001711114363 19.398070493408834 24-25 20.795879886302874 27.78206174541975 32.22638111051698 19.19567725776039 26-27 20.199365093659942 28.01467939481268 32.31324297550432 19.472712536023053 28-29 19.832938381956446 28.585098418881117 32.07042705407551 19.511536145086936 30-31 20.24810599664539 28.051512388414217 32.0798577105355 19.620523904404894 32-33 20.08262695169588 28.542883838213278 31.775916607567005 19.598572602523838 34-35 20.159175081332386 28.448324383956415 31.89411592538809 19.49838460932311 36-37 20.665743586279874 28.162394605608277 31.708374140240675 19.463487667871174 38-39 20.133846656085012 28.814179415307372 31.595240507469068 19.45673342113854 40-41 19.8754967185618 29.120371933853413 31.408373014532888 19.595758333051908 42-43 20.367482732026954 29.25225595442494 30.610613540792947 19.769647772755164 44-45 20.236286064863283 29.143448943523243 30.483604066056536 20.136660925556942 46-47 20.59426114169284 29.79748516879988 29.764839642925487 19.84341404658179 48-49 20.952799072416784 29.685477243817047 29.149640336361486 20.21208334740468 50-51 20.555424222980196 29.69842288338793 29.36859050127768 20.377562392354193 52-53 20.549232830141953 30.039512343385905 28.963335697319692 20.447919129152456 54-55 20.26724302905452 30.02656670381502 28.872153366429142 20.834036900701317 56-57 20.431033512320873 29.576846442200534 29.091103531345336 20.90101651413326 58-59 20.570621278128623 28.98359843751759 29.089977823556563 21.35580246079723 60-61 20.02634156225727 28.79785665237018 30.093546317246968 21.082255468125584 62-63 19.766303063050895 28.146071842671077 30.55621221843234 21.53141287584569 64-65 19.205137730347957 28.126371956367567 30.785856607341866 21.88263370594261 66-67 19.210766269291817 27.79879098983486 30.615311877342876 22.375130863530444 68-69 19.188252113516373 27.430121689011965 30.355273378136506 23.02635281933516 70-71 18.983373295959836 27.89785327524681 30.25227111546385 22.866502313329505 72-73 18.636092443123616 27.31192237119089 30.602366237772 23.4496189479135 74-75 18.151475240057184 27.16220323528418 29.920187317776055 24.76613420688258 76-77 17.973050555536794 26.219422962187476 30.743642565262906 25.06388391701282 78-79 18.26573458061756 26.695597356838114 30.329944952889125 24.708723109655196 80-81 17.78899733207254 26.028615491990585 29.69223149054968 26.490155685387183 82-83 17.188995080656962 25.72073441176139 29.91455877883219 27.17571172874945 84-85 16.855222721286008 25.70103452545788 30.20949421949051 27.234248533765605 86-87 17.04096450643342 25.72748865849403 29.193542940123603 28.03800389494895 88-89 15.932142334492813 25.11172649803564 28.968401382369162 29.987729785102385 90-91 16.266620132172974 25.42359523997163 29.455771590691597 28.8540130371638 92-93 15.90877110034166 26.20466838902867 28.471882158918856 29.414678351710823 94-95 14.985422084135399 26.996724190334675 26.910607544493597 31.107246181036324 96-97 15.62988979320748 28.46127002352729 25.82204811275089 30.086792070514335 98-99 15.957470759740186 28.969527090157936 24.825233865793116 30.24776828430876 100-101 15.560684530511146 26.42141409592434 24.08860616978158 33.929295203782935 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 54.0 1 40.0 2 29.5 3 26.5 4 20.5 5 26.5 6 38.0 7 54.5 8 91.5 9 123.0 10 132.5 11 141.0 12 159.0 13 178.0 14 199.0 15 212.0 16 221.5 17 248.0 18 274.5 19 294.5 20 308.5 21 368.0 22 417.0 23 462.0 24 534.5 25 625.5 26 731.0 27 836.5 28 972.0 29 1142.0 30 1335.5 31 1557.0 32 1729.0 33 1847.5 34 2012.0 35 2202.5 36 2450.5 37 2635.5 38 2719.5 39 2782.5 40 2861.0 41 3000.5 42 3134.0 43 3219.5 44 3360.0 45 3407.5 46 3386.0 47 3553.5 48 3783.0 49 3652.0 50 3326.0 51 3042.5 52 2819.5 53 2705.0 54 2445.0 55 2161.0 56 1862.0 57 1541.5 58 1308.0 59 1106.5 60 859.5 61 610.5 62 440.5 63 317.0 64 226.5 65 148.5 66 102.0 67 82.5 68 53.0 69 29.5 70 22.0 71 17.0 72 11.5 73 9.0 74 7.0 75 5.5 76 4.5 77 2.0 78 0.5 79 0.5 80 1.0 81 1.0 82 0.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05741109722738172 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004502831155088763 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0022514155775443813 24-25 5.628538943860953E-4 26-27 0.0011257077887721907 28-29 0.003939977260702667 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01407134735965238 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011257077887721905 92-93 0.0016885616831582857 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.014634201254038475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 88833.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.57740929609491 #Duplication Level Percentage of deduplicated Percentage of total 1 90.88992941109902 72.32785113640202 2 5.630136792519557 8.960633998626637 3 1.5051420972966856 3.593259261760832 4 0.6337440409670255 2.0172683574797654 5 0.37487091708986997 1.4915628201231526 6 0.24189783706553875 1.1549761912802674 7 0.1513629740702494 0.8431551337903708 8 0.10892475704120749 0.6934359978836693 9 0.08770564852668657 0.6281449461348824 >10 0.3550664158096504 4.9902626276271205 >50 0.012731465108712566 0.720452984814202 >100 0.005658428937205585 1.1031936329967467 >500 0.0028292144686027925 1.4758029110803417 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.7789897898303558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 619 0.696813121249986 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.3928720182814945 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 253 0.2848040705593642 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 190 0.2138844798667162 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 188 0.2116330642891718 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.10356511656704151 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 89 0.10018799320072495 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 89 0.10018799320072495 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0011257077887721905 0.0 20-21 0.0 0.0 0.0 0.0011257077887721905 0.0 22-23 0.0 0.0 0.0 0.0011257077887721905 0.0 24-25 0.0 0.0 0.0 0.002251415577544381 0.0 26-27 0.0 0.0 0.0 0.005065685049474857 0.0 28-29 0.0 0.0 0.0 0.010131370098949714 0.0 30-31 0.0 0.0 0.0 0.029268402508076954 0.0 32-33 0.0 0.0 0.0 0.041651188184571045 0.0 34-35 0.0 0.0 0.0 0.05009399660036248 0.0 36-37 0.0 0.0 0.0 0.06191392838247048 0.0 38-39 0.0 0.0 0.0 0.10018799320072497 0.0 40-41 0.0 0.0 0.0 0.18011324620355046 0.0 42-43 0.0 0.0 0.0 0.37429783976675335 0.0 44-45 0.0 0.0 0.0 0.5825537806896086 0.0 46-47 0.0 0.0 0.0 1.0598538831290174 0.0 48-49 0.0 0.0 0.0 1.5281483232582487 0.0 50-51 0.0 0.0 0.0 1.884997692299033 0.0 52-53 0.0 0.0 0.0 2.305449551405446 0.0 54-55 0.0 0.0 0.0 2.7388470500827395 0.0 56-57 0.0 0.0 0.0 3.842040683079486 0.0 58-59 0.0 0.0 0.0 5.146736010266455 0.0 60-61 0.0 0.0 0.0 6.538110837188882 0.0 62-63 0.0 0.0 0.0 8.150124390710658 0.0 64-65 0.0 0.0 0.0 9.820111895354204 0.0 66-67 0.0 0.0 0.0 11.780532009500973 0.0 68-69 0.0 0.0 0.0 13.792734681931265 0.0 70-71 0.0 0.0 0.0 15.851654227595603 0.0 72-73 0.0 0.0 0.0 17.94772213028942 0.0 74-75 0.0 0.0 0.0 20.037598640144992 0.0 76-77 0.0 0.0 0.0 22.90083640088706 0.0 78-79 0.0 0.0 0.0 25.09596658899283 0.0 80-81 0.0 0.0 0.0 27.713800051782556 0.0 82-83 0.0 0.0 0.0 30.941767136086817 0.0 84-85 0.0 0.0 0.0 34.862044510485966 0.0 86-87 0.0 0.0 0.0 37.72190514786172 0.0 88-89 0.0 0.0 0.0 40.85700133959227 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGG 25 5.2485655E-5 76.0 2 CTTGGTA 20 0.0020677606 71.25 2 TAAGGGT 30 1.2946523E-4 63.33333 3 TCCGTTC 25 0.0050060716 57.0 8 AGCAGTA 25 0.0050060716 57.0 5 AAGGGTA 35 2.7739216E-4 54.285717 4 GCTAAGG 35 2.7739216E-4 54.285717 1 AGGGTAG 45 1.5045658E-5 52.777775 5 GGGTAGT 45 9.5770956E-4 42.22222 6 ACAGGTG 45 9.5770956E-4 42.22222 5 TAGTTTA 50 0.0016077975 38.0 9 CATGGGC 65 1.3183383E-4 36.538464 4 GGTAGTT 55 0.0025668812 34.545452 7 ATGGGAC 75 3.0557942E-4 31.666668 5 ATGGGAG 120 1.4794205E-7 31.666664 5 GGTGTGC 60 0.0039315205 31.666664 8 GATCAGG 60 0.0039315205 31.666664 1 TTGGTAC 30 0.0039420426 31.666664 46-47 AGTAGAT 60 0.0039315205 31.666664 2 GGTATCA 620 0.0 31.411291 1 >>END_MODULE